| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 654,403 | T→G | 6.1% | K114Q (AAG→CAG) | ctpL ← | methyl‑accepting chemotaxis protein CtpL |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 654,403 | 0 | T | G | 6.1% | 350.0 / 9.9 | 110 | K114Q (AAG→CAG) | ctpL | methyl‑accepting chemotaxis protein CtpL |
| Reads supporting (aligned to +/- strand): ref base T (48/55); new base G (4/3); total (52/58) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.05e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 112 total aligned reads displayed.
AGATGCAGGGTGGCGTCTAGCAAGGGCAGCAAGTAGCGATTGGCCTCGGCGTTGCCACTGTCGCGGGCGTAGCTGGCCATCTGCTCGAAGTTCGCCCCCAG‑CTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCAACTTCTCGGCCAGCTCTACCGGCCAGTCCGTCAGTGCGGCCTGCAACTGTTGGTTCGCCTCCATGGCGGCGGCCTGGCGCAGGGCG > NC_002947/654255‑654548 | aGATGCAGGGTGGCGTCTAGCAAGGGCAGCAAGTAGCGATTGGCCTCGGCGTTGCCACTGTCGCGGGCGTAGCTGGCCATCTGCTCGAAGTTCGCCCCCAG‑CTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCtt > 8:28902/1‑149 (MQ=255)aGATGCAGGGTGGCGTCTAGCAAGGGCAGCAAGTAGCGATTGGCCTCGGCGTTGCCACTGTCGCGGGCGTAGCTGGCCATCTGCTCGAAGTTCGCCCCCAG‑CTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCtt < 8:322812/149‑1 (MQ=255) aTGCAGGGTGGCGTCTAGCAAGGGCAGCAAGTAGCGATTGGCCTCGGCGTTGCCACTGTCGCGGGCGTAGCTGGCCATCTGATCGAAGTTCGCCCCCAG‑CTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTCGGTCGCCGGCCAGCTTGc > 2:221409/1‑149 (MQ=255) tGGCGTCTAGCAAGGGCAGCAAGTAGCGATTGGCCTCGGCGTTGCCACTGTCGCGGGCGTAGCTGGCCATCTGCTCGAAGTTCGCCCCCAG‑CTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGCCGCCGGCAAGCTGGCCGGCGAcc < 4:234309/149‑1 (MQ=255) ggCGTCTAGCAAGGGCAGCAAGTAGCGATTGGCCTCGGCGTTGCCACTGTCGCGGGCGTAGCTGGCCATCTGCTCGAAGTTCGCCCCCAG‑CTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCca < 7:201640/149‑1 (MQ=255) tAGCAAGGGCAGCAAGTAGCGATTGGCCTCGGCGTTGCCACTGTCGCGGGCGTAGCTGGCCATCTGCTCGAAGTTCGCCCCCAG‑CTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGt‑t > 6:31568/1‑149 (MQ=255) aGCAAGGGCAGCAAGTAGCGATTGGCCTCGGCGTTGCCACTGTCGCGGGCGTAGCTGGCCATCTGCTCGAAGTTCGCCCCCAG‑CTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGT‑Tc > 5:278947/1‑149 (MQ=255) cAAGGGCAGCAAGTAGCGATTGGCCTCGGCGTTGCCACTGTCGCGGGCGTAGCTGGCCATCTGCTCGAAGTTCGCCCCCAG‑CTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGT‑TCGt < 2:77671/149‑1 (MQ=255) cAAGGGCAGCAAGTAGCGATTGGCCTCGGCGTTGCCACTGTCGCGGGCGTAGCTGGCCATCTGCTCGAAGTTCGCCCCCAG‑CTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGT‑TCGt > 4:225161/1‑149 (MQ=255) aaGGGCAGCAAGTAGCGATTGGCCTCGGCGTTGCCACTGTCGCGGGCGTAGCTGGCCATCTGCTCGAAGTTCGCCCCCAG‑CTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGT‑TCGtt < 1:167190/149‑1 (MQ=255) aaGGGCAGCAAGTAGCGATTGGCCTCGGCGTTGCCACTGTCGCGGGCGTAGCTGGCCATCTGCTCGAAGTTCGCCCCCAG‑CTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGT‑TCGtt > 5:296985/1‑149 (MQ=255) gcagcaAGTAGCGATTGGCCTCGGCGTTGCCACTGTCGCGGGCGTAGCTGGCCATCTGCTCGAAGTTCGCCCCCAG‑CTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCg < 4:132834/149‑1 (MQ=255) gcagcaAGTAGCGATTGGCCTCGGCGTTGCCACTGTCGCGGGCGTAGCTGGCCATCTGCTCGAAGTTCGCCCCCAG‑CTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCg < 2:277301/149‑1 (MQ=255) cagcaAGTAGCGATTGGCCTCGGCGTTGCCACTGTCGCGGGCGTAGCTGGCCATCTGCTCGGAGTTCGCCCCCAG‑CTCCCGCTCGGCTTGCAGCAGCAGGGCTTGGGGGTCGCCGGCCAGCTTGCCGGCGGCGAGGAGT‑TCGTTGGCgg > 8:126708/1‑149 (MQ=255) aGTAGCGATTGGCCTCGGCGTTGCCACTGTCGCGGGCGTAGCTGGCCATCTGCTCGAAGTTCGCCCCCAG‑CTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCGGTGAAg < 3:357711/149‑1 (MQ=255) gTAGCGATTGGCCTCGGCGTTGCCACTGTCGCGGGCGTAGCTGGCCATCTGCTCGAAGTTCGCCCCCAG‑CTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCg < 5:231674/142‑1 (MQ=255) gTAGCGATTGGCCTCGGCGTTGCCACTGTCGCGGGCGTAGCTGGCCATCTGCTCGAAGTTCGCCCCCAG‑CTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCg > 6:231674/1‑142 (MQ=255) gTAGCGATTGGCCTCGGCGTTGCCACTGTCGCGGGCGTAGCTGGCCATCTGCTCGAAGTTCGCCCCCAG‑CTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCGGTGAAgg < 8:183993/149‑1 (MQ=255) tAGCGATTGGCCTCGGCGTTGCCACTGTCGCGGGCGTAGCTGGCCATCTGCTCGAAGTTCGCCCCCAG‑CTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCGGTGAAGGc < 8:316047/149‑1 (MQ=255) tAGCGATTGGCCTCGGCGTTGCCACTGTCGCGGGCGTAGCTGGCCATCTGCTCGAAGTTCGCCCCCAG‑CTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCGGTGAAGGc < 2:137925/149‑1 (MQ=255) gATTGGCCTCGGCGTTGCCACTGTCGCGGGCGTAGCTGGCCATCTGCTCGAAGTTCGCCCCCAG‑CTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCGGTGAAGGCCTGc < 6:278947/149‑1 (MQ=255) aTTGGCCTCGGCGTTGCCACTGTCGCGGGCGTAGCTGGCCATCTGCTCGAAGTTCGCCCCCAG‑CTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCcagca > 4:307168/1‑125 (MQ=255) aTTGGCCTCGGCGTTGCCACTGTCGCGGGCGTAGCTGGCCATCTGCTCGAAGTTCGCCCCCAG‑CTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCcagca < 3:307168/125‑1 (MQ=255) aTTGGCCTCGGCGTTGCCACTGTCGCGGGCGTAGCTGGCCATCTGCTCGAAGTTCGCCCCCAG‑CTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCGGt < 6:267003/138‑1 (MQ=255) aTTGGCCTCGGCGTTGCCACTGTCGCGGGCGTAGCTGGCCATCTGCTCGAAGTTCGCCCCCAG‑CTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCGGt > 5:267003/1‑138 (MQ=255) aTTGGCCTCGGCGTTGCCACTGTCGCGGGCGTAGCTGGCCATCTGCTCGAAGTTCGCCCCCAG‑CTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCGGTGAAGGCCTGCa < 3:164953/149‑1 (MQ=255) gCCTCGGCGTTGCCACTGTCGCGGGCGTAGCTGGCCATCTGCTCGAAGTTCGCCCCCAG‑CTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCGGTGAAGGCCTGCAGGc > 1:178598/1‑148 (MQ=255) ccTCGGCGTTGCCACTGTCGCGGGCGTAGCTGGCCATCTGCTCGAAGTTCGCCCCCAG‑CTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCGGTGAAGGCCTGCAGGCTa < 3:226298/149‑1 (MQ=255) cTCGGCGTTGCCACTGTCGCGGGCGTAGCTGGCCATCTGCTCGAAGTTCGCCCCCAG‑CTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCGGTGAAGGCCTGCAGGCTAt > 6:217416/1‑149 (MQ=255) tCGGCGTTGCCACTGTCGCGGGCGTAGCTGGCCATCTGCTCGAAGTTCGCCCCCAG‑CTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCGGTGAAGGCCTGCAGGCTATc > 4:35775/1‑149 (MQ=255) cGTTGCCACTGTCGCGGGCGTAGCTGGCCATCTGCTCGAAGTTCGCCCCCAG‑CTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCGGTGAAGGCCTGCAGGCTATCCAgg > 6:102144/1‑149 (MQ=255) cACTGTCGCGGGCGTAGCTGGCCATCTGCTCGAAGTTCGCCCCCAG‑CTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTgggg > 6:78538/1‑149 (MQ=255) aCTGTCGCGGGCGTAGCTGGCCATCTGCTCGAAGTTCGCCCCCAGCCTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTgggg > 1:268874/1‑149 (MQ=255) aCTGTCGCGGGCGTAGCTGGCCATCTGCTCGAAGTTCGCCCCCAG‑CTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTggg > 6:53807/1‑147 (MQ=255) aCTGTCGCGGGCGTAGCTGGCCATCTGCTCGAAGTTCGCCCCCAG‑CTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTggg < 5:53807/147‑1 (MQ=255) aCTGTCGCGGGCGTAGCTGGCCATCTGCTCGAAGTTCGCCCCCAG‑CTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGgc > 4:71037/1‑149 (MQ=255) tGTCGCGGGCGTAGCTGGCCATCTGCTCGAAGTTCGCCCCCAG‑CTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGgcgc < 8:320392/149‑1 (MQ=255) tCGCGGGCGTAGCTGGCCATCTGCTCGAAGTTCGCCCCCAG‑CTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCaa < 5:217416/149‑1 (MQ=255) tCGCGGGCGTAGCTGGCCATCTGCTCGAAGTTCGCCCCCAG‑CTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCaa < 5:39504/149‑1 (MQ=255) gcgGGCGTAGCTGGCCATCTGCTCGAAGTTCGCCCCCAG‑CTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCAACt > 5:261296/1‑149 (MQ=255) gcgGGCGTAGCTGGCCATCTGCTCGAAGTTCGCCCCCAG‑CTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCAACt > 4:133423/1‑149 (MQ=255) gCGTAGCTGGCCATCTGCTCGAAGTTCGCCCCCAG‑CTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCAACTtctc < 7:205884/149‑1 (MQ=255) cGTAGCTGGCCATCTGCTCGAAGTTCGCCCCCAG‑CTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCAACTTCTCg < 6:261296/149‑1 (MQ=255) aGCTGGCCATCTGCTCGAAGTTCGCCCCCAG‑CTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGTCTCGTTGGCGGTGAAGGCCTGCAGGCTATcc < 1:34914/126‑1 (MQ=255) aGCTGGCCATCTGCTCGAAGTTCGCCCCCAG‑CTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCGGTGAAGGCCTGCAGGCTATcc > 2:34914/1‑125 (MQ=255) gCTGGCCATCTGCTCGAAGTTCGCCCCCAG‑CTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCAACTTCTCGGcc < 1:321703/148‑1 (MQ=255) tGGCCATCTGCTCGAAGTTCGCCCCCAG‑CTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCAACTtct > 6:121962/1‑141 (MQ=255) tGGCCATCTGCTCGAAGTTCGCCCCCAG‑CTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCAACTtct < 5:121962/141‑1 (MQ=255) tGGCAATCTGCTCGAAGTTCGCCCCCAG‑CTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCAACTTCTCGGCCAGc > 1:68624/1‑149 (MQ=255) gCCATCTGCTCGAAGTTCGCCCCCAG‑CTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCAACTTCTCGGCCAGctc < 7:28902/149‑1 (MQ=255) ccATCTGCTCGAAGTTCGCCCCCAG‑CTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCAACTTCTCGGCCAGctc > 1:211653/1‑148 (MQ=255) aTCTGCTCGAAGTTCGCCCCCAG‑CTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCAACTTCTCGGCCAGCTCTAc < 5:102144/149‑1 (MQ=255) cTGCTCGAAGTTCGCCCCCAG‑CTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCAACTTCTCGGCCAGCTCTACCg > 8:190440/1‑149 (MQ=255) gCTCGAAGTTCGCCCCCAG‑CTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCGGTGAAGGCCt < 5:321261/102‑1 (MQ=255) gCTCGAAGTTCGCCCCCAG‑CTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCGGTGAAGGCCt > 6:321261/1‑102 (MQ=255) cTCGAAGTTCGCCCCCAG‑CTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCAACTTCTCGGCCAGCTCTACCGGc < 1:304175/148‑1 (MQ=255) cTCGAAGTTCGCCCCCAG‑CTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCAACTTCTCGGCCAGCTCTACCGGc > 7:249010/1‑148 (MQ=255) tCGAAGTTCGCCCCCAG‑CTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCAACTTCTCGGCCAGCTCTACCGGc > 8:344686/1‑147 (MQ=255) aGTTCGCCCCCAG‑CTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCAACTTCTCGGCCAGCTCTACCGGCCAGTcc > 5:158279/1‑149 (MQ=255) aGTTCGCCCCCAG‑CTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCAACTTCTCGGCCAGCTCTACCGGCCAGTcc < 2:211653/149‑1 (MQ=255) gTTCGCCCCCAG‑CTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCCGCTTGCCGGCGGCCGGCAGT‑GCGTTGGCGGGGAAGGCCGGCCGGCTATCCAGGGGGGGGCGCAACTGGGCGGCCAGGGGTCCCGGCCAGTCCg > 7:4445/1‑149 (MQ=255) ttCGCCCCCAG‑CTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCAACTTCTCGGCCAGCTCTACCGGCCAGTCCGt > 5:33740/1‑149 (MQ=255) ttCGCCCCCAG‑CTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCAACTTCTCGGCCAGCTCTACCGGCCAGTCCGt < 6:33740/149‑1 (MQ=255) gCCCCCAG‑CTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCAACTTCTCGGCCAGCTCTACCGGCCAGTCCGTCa < 3:35775/148‑1 (MQ=255) cccAG‑CTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGT‑TCGTTgg < 4:344004/76‑1 (MQ=255) cccAG‑CTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGT‑TCGTTgg > 3:344004/1‑76 (MQ=255) cccAG‑CTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCAACTTCTCGGCCAGCTCTACCGGCCAGTCCGTCAGTGc > 2:46603/1‑149 (MQ=255) ccAG‑CTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCAACTTCTCGGCCAGCTCTACCGGCCAGTCCGTCAGTGCg < 4:106274/149‑1 (MQ=255) cTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCAACTTCTCGGCCAGCTCTACCGGCCAGTCCGTCAGTGCGGCCt < 5:78538/149‑1 (MQ=255) cTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGACCAGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCAACTTCTCGGCCAGCTCTACCGGCCAGTCCGTCAGTGCGGCCt > 5:204825/1‑149 (MQ=255) cGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCAACTTCTCGGCCAGCTCTACCGGCCAGTCCGTCAGTGCGGCCTGCaa < 2:268874/149‑1 (MQ=255) ggCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCGGTGAAGGCCTGCAGTCTATCCAGGCTGGGGCGCAACTTCTCGGCCAGCTCTACCGGCCAGTCCGTCAGTGCGGCCTGCAACTGt < 2:68624/148‑1 (MQ=255) gCTTGCAGCAGGAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGT‑TCGGGGCCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCAACTTCTCGGCCAGCTCTACCGGCCAGTCCGTCAGTGCGGCCTGCAACTGTTg < 7:84828/149‑1 (MQ=255) gCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCAACTTCTCGGCCAGCTCTACCGGCCAGTCCGTCAGTGCGGCCTGCAACTGTTg < 7:190440/149‑1 (MQ=255) gCTTGCAGCAGCAGGGCTTGTGGGTCGCCGACCAGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCAACTTCTCGGCCAGCTCTACCGGCCAGTCCGTCAGTGCGGCCTGCAACTGTTg < 6:204825/149‑1 (MQ=255) gcagcagcaGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCAACTTCTCGGCCAGCTCTACCGGCCAGTCCGTCAGTGCGGCCTGCAACTGTTGGTTc > 8:305089/1‑149 (MQ=255) cagcagcagGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCAACTTCTCGGCCAGCTCTACCGGCCAGTCCGTCAGTGCGGCCTGCAACTGTTGGTTc < 3:83564/148‑1 (MQ=255) agcagcagGGCTTGTGGGTCGCCGGCCAGCTTGCAGGCGGCCAGCAGT‑TCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCACCTTCTCGGCCAGCTCTACCGGCCAGTCCGTCAGTGCGGCCTGCACCTGTTGGTTCGc > 7:249458/1‑149 (MQ=255) gcagcagGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCAACTTCTCGGCCAGCTCTACCGGCCAGTCCGTCAGTGCGGCCTGCAACTGTTg < 3:63357/142‑1 (MQ=255) gcagcagGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCAACTTCTCGGCCAGCTCTACCGGCCAGTCCGTCAGTGCGGCCTGCAACTGTTg > 4:63357/1‑142 (MQ=255) gcagcagGGCTTGTGGGTCGCCGGCCAGCTTGACGGCGGCCAGCAGT‑TCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCAACTTCTCGGCCAGCTCTACCGGCCAGTCCGTCAGTGCGGCCTGCAACTGTTGGTTCGcc < 1:221409/149‑1 (MQ=255) gcagGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCAACTTCTCGGCCAGCTCTACCGGCCAGTCCGTCAGTGCGGCCTGCAACTGTTGGTTCGCCTcc < 2:178598/149‑1 (MQ=255) ttGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCAACTTCTCGGCCAGCTCTACCGGCCAGTCCGTCAGTGCGGCCTGCAACTGtt > 3:294902/1‑131 (MQ=255) ttGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCAACTTCTCGGCCAGCTCTACCGGCCAGTCCGTCAGTGCGGCCTGCAACTGTTGGTTCGCCTCCATggcgg > 5:304504/1‑149 (MQ=255) gtgGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCAACTTCTCGGCCAGCTCTACCGGCCAGTCCGTCAGTGCGGCCTGCAACTGTTGGTTCGCCTCCATggcggcg > 7:202897/1‑149 (MQ=255) ctggccGCCGGCAAGCTGGCCGGCGACCAGCAGT‑TCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCAACTTCTCGGCCAGCTCTACCGGCCAGTCCGTCAGTGCGGCCTGCAACTGTTGGTTc < 4:337292/128‑1 (MQ=255) ctggccGCCGGCAAGCTGGCCGGCGACCAGCAGT‑TCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCAACTTCTCGGCCAGCTCTACCGGCCAGTCCGTCAGTGCGGCCTGCAACTGTTGGTTc > 3:337292/6‑133 (MQ=255) tggccGCCGGCAAGCTGGCCGGCGACCAGCAGT‑TCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCAACTTCTCGGCCAGCTCTACCGGCCAGTCCGTCAGTGCGGCCTGCAACTGTTGGTTCGCCTCCa > 2:45925/5‑139 (MQ=255) ggTCGCCGGTCAGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCAACTTCTCGGCCAGCTCTACCGGCCAGTCCGTCAGTGCGGCCTGCAACTGTTGGTTCGCCTCCATGGCGGCGGcc > 7:96240/1‑149 (MQ=255) gCCGGCCAGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCAACTTCTCGGCCAGCTCTACCGGCCAGTCCGTCAGTGCGGCCTGCAACTGTTGGTTCGCCTCCATGGCGGCGGCCTGgc < 1:185020/149‑1 (MQ=255) gCCGGCCAGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCAACTTCTCGGCCAGCTCTACCGGCCAGTCCGTCAGTGCGGCCTGCAACTGTTGGTTCGCCTCCATGGCGGCGGCCTGgc > 7:109247/1‑149 (MQ=255) ccGGCCAGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCAACTTCTCGGCCAGCTCTACCGGCCAGTCCGTCAGTGCGGCCTGCAACTGTTGGTTCGCCTCCATGGCGGCGGCCTGgcg > 2:55304/1‑149 (MQ=255) gCCAGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCAACTTCTCGGCCAGCTCTACCGGCCAGTCCGTCAGTGCGGCCTGCAACTGTTGGTTCGCCTCCATGGCGGCGGCCTGGCGCAg < 7:305089/149‑1 (MQ=255) ccAGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCAACTTCTCGGCCAGCTCTACCGGCCAGTCCGTCAGTGCGGCCTGCAACTGTTGGTTCGCCTCCATGGCGGCGGCCTGGCGCAgg < 7:306850/149‑1 (MQ=255) ccAGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCAACTTCTCGGCCAGCTCTACCGGCCAGTCCGTCAGTGCGGCCTGCAACTGTTGGTTCGCCTCCATGGCGGCGGCCTGGCGCAgg > 8:306850/1‑149 (MQ=255) ccAGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCAACTTCTCGGCCAGCTCTACCGGCCAGTCCGTCAGTGCGGCCTGCAACTGTTGGTTCGCCTCCATGGCGGCGGCCTGGCGCAg < 2:82623/148‑1 (MQ=255) ccAGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCAACTTCTCGGCCAGCTCTACCGGCCAGTCCGTCAGTGCGGCCTGCAACTGTTGGTTCGCCTCCATGGCGGCGGCCTGGCGCAg > 1:82623/1‑148 (MQ=255) aGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCAACTTCTCGGCCAGCTCTACCGGCCAGTCCGTCAGTGCGGCCTGCAACTGTTGGTTCGCCTCCATGGCGGCGGCCTGGCGCAGGGc < 3:246021/149‑1 (MQ=255) aGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCAACTTCTCGGCCAGCTCTACCGGCCAGTCCGTCAGTGCGGCCTGCAACTGTTGGTTCGCCTCCATGGCGGCGGCCTGGCGCAGGGc < 3:71037/149‑1 (MQ=255) aGCTGGCCGGCGACCAGCAGT‑TCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCAACTTCTCGGCCAGCTCTACCGGCCAGTCCGTCAGTGCGGCCTGCAACTGTTGGTTCGCCTCCATGGCGGCGGCCTGGCGCAGGGc > 3:32819/1‑149 (MQ=255) | AGATGCAGGGTGGCGTCTAGCAAGGGCAGCAAGTAGCGATTGGCCTCGGCGTTGCCACTGTCGCGGGCGTAGCTGGCCATCTGCTCGAAGTTCGCCCCCAG‑CTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGT‑TCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCAACTTCTCGGCCAGCTCTACCGGCCAGTCCGTCAGTGCGGCCTGCAACTGTTGGTTCGCCTCCATGGCGGCGGCCTGGCGCAGGGCG > NC_002947/654255‑654548 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |