Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	689,975	C→T	5.4%	R221H (CGC→CAC)	PP_0591 ←	adenosine deaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	689,975	0	C	T	5.4%	284.4 / 2.5	92	R221H (CGC→CAC)	PP_0591	adenosine deaminase
Reads supporting (aligned to +/- strand):  ref base C (43/44);  new base T (3/2);  total (46/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTTTCACACCACGCTCGAGCATGTCGAGGATGTTGTGCTGGCTCATATGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATGGGCAACGGCGACGAAGCCTTCGCTACGGGCGCGGTCGAACACCCGCTGGAACTTGCTGGGTGGGT  >  NC_002947/689829‑690122                                                                                                                                                   |                                                                                                                                                     cTTTCACACCACGCTCGAGCATGTCGAGGATGTTGTGCTGGCTCATATGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGgcgcgc                                                                                                                                                    <  6:238067/149‑1 (MQ=255)  tttCACACCACGCTCGAGCATGTCGAGGATGTTGTGCTGGCTCATATGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCa                                                                                                                                                   >  1:136205/1‑149 (MQ=255)    tCACACCACGCTCGAGCATGTCGAGGATGTTGTGCTGGCTCATATGGTCTAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCTGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCAc                                                                                                                                                  <  1:42537/148‑1 (MQ=255)      acacCACGCTCGAGCATGTCGAGGATGTTGTGCTGGCTCATATGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGc                                                                                                                                                >  8:340727/1‑148 (MQ=255)      acacCACGCTCGAGCATGTCGAGGATGTTGTGCTGGCTCATATGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGc                                                                                                                                                >  6:372938/1‑148 (MQ=255)        accacGCTCGAGCATGTCGAGGATGTTGTGCTGGCTCATATGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGTGCGCGCCAt                                                                                                                                             <  5:141696/149‑1 (MQ=255)        accacGCTCGAGCATGTCGAGGATGTTGTGCTGGCTCATATGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCAt                                                                                                                                             >  6:101438/1‑149 (MQ=255)        accacGCTCGAGCATGTCGAGGATGTTGTGCTGGCTCATATGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCAt                                                                                                                                             >  2:32395/1‑149 (MQ=255)          tacGCTCGAGCATGTCGAGGATGTTGTGCTGGCTCATATGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATgg                                                                                                                                           >  4:208124/2‑149 (MQ=255)          cacGCTCGAGCATGTCGAGGATGTTGTGCTGGCTCATATGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATgg                                                                                                                                           >  6:244538/1‑149 (MQ=255)                 gAGCATGTCGAGGATGTTGTGCTGGCTCATATGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATAc                                                                                                                                    <  1:338890/149‑1 (MQ=255)                   gCATGTCGAGGATGTTGTGCTGGCTCATATGGTCGAACACGCAGAGATAGGTGTTGGACAGAGGGCACACAGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTAGTCCTCCATGGCGCGCACGCCATTGTCGATAAGt                                                                                                                                  >  7:234296/1‑149 (MQ=255)                    cATGTCGAGGATGTTGTGCTGGCTCATATGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGtt                                                                                                                                 <  3:351560/149‑1 (MQ=255)                     aTGTCGAGGATGTTGTGCTGGCTCATATGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGttt                                                                                                                                <  2:40638/149‑1 (MQ=255)                       gTCGAGGATGTTGTGCTGGCTCATATGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTg                                                                                                                               <  2:136205/148‑1 (MQ=255)                       gTCGAGGATGTTGTGCTGGCTCATATGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTAGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTg                                                                                                                               >  3:287664/1‑148 (MQ=255)                        tCGAGGATGTTGTGCTGGCTCATATGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGa                                                                                                                              <  3:208124/148‑1 (MQ=255)                        tCGAGGATGTTGTGCTGGCTCATATGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGATCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGa                                                                                                                              >  6:227273/1‑148 (MQ=255)                           aGGATGTTGTGCTGGCTCATATGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCtt                                                                                                                          <  7:92063/149‑1 (MQ=255)                              aTGTTGTGCTGGCTCATATGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTcag                                                                                                                       <  7:109167/149‑1 (MQ=255)                               tgttgtGCTGGCTCATATGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTcagc                                                                                                                      <  4:72889/149‑1 (MQ=255)                                gttgtGCTGGGTCATTCGGTCGAAGACGCAGAGCTTGGTGTTGGACAGCTGGCACTCCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGGTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTcagc                                                                                                                      <  7:277219/148‑1 (MQ=255)                                gttgtGCTGGCTCATATGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGAt                                                                                                                                      >  6:59440/1‑132 (MQ=255)                                gttgtGCTGGCTCATATGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGAt                                                                                                                                      <  5:59440/132‑1 (MQ=255)                                   gtgCTGGCTCATATGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGt                                                                                                                  >  5:112701/1‑149 (MQ=255)                                      cTGGCTCATATGGTCGAACACGCAGAGCTTGGAGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTc                                                                                                                 >  3:146545/1‑147 (MQ=255)                                         gCTCATATGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAg                                                                                                              <  3:365119/147‑1 (MQ=255)                                           tCATATGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCt                                                                                                          >  5:63836/1‑149 (MQ=255)                                              tatGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGc                                                                                                           <  3:230036/145‑1 (MQ=255)                                              tatGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGc                                                                                                           >  4:230036/1‑145 (MQ=255)                                              tatGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTcc                                                                                                       <  7:158743/149‑1 (MQ=255)                                                  gTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAg                                                                                                    <  5:227273/148‑1 (MQ=255)                                                  gTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGa                                                                                                   <  6:63836/149‑1 (MQ=255)                                                      aaCACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTa                                                                                               >  3:127002/1‑149 (MQ=255)                                                         acGCAGAGCTTGGTGTTGGACAGCGGGCTCACCGGGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTc                                                                                            <  8:188396/149‑1 (MQ=255)                                                          cgctgttcTTGGTGTTGTTGAGCGGTCAGAGCGTTAGTGGGATCTGCTCGTCTTTGATGCGCTGCTTCAGGTGGTCGTTGTTGATGTCGCTTACGCCATTGTCTATATGTTTGATCTTCAGCAGGTCGAGGGCTTCCCTGATGTACTc                                                                                            <  8:234296/141‑1 (MQ=255)                                                           gCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCCGATGTACTcgg                                                                                          >  7:355937/1‑149 (MQ=255)                                                           gCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTcgg                                                                                          >  8:288583/1‑149 (MQ=255)                                                           gCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTcgg                                                                                          <  2:182424/149‑1 (MQ=255)                                                            cAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTcggg                                                                                         <  5:101438/149‑1 (MQ=255)                                                             agagCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCGATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTcgggc                                                                                        >  5:342446/1‑149 (MQ=255)                                                               agCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTcgggcgg                                                                                      <  2:372228/149‑1 (MQ=255)                                                                     gtgtTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCcttc                                                                                >  1:56080/1‑149 (MQ=255)                                                                     gtgtTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCcttc                                                                                <  2:56080/149‑1 (MQ=255)                                                                          ggACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCcttc                                                                                <  7:178959/144‑1 (MQ=255)                                                                          ggACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCcttc                                                                                >  8:178959/1‑144 (MQ=255)                                                                          ggACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGc                                                                          >  1:62157/1‑149 (MQ=255)                                                                           gACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCTGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGc                                                                          <  4:146545/148‑1 (MQ=255)                                                                           gACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGc                                                                          >  2:216728/1‑148 (MQ=255)                                                                                  ggCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATgg                                                                  <  5:372938/149‑1 (MQ=255)                                                                                   gCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATggg                                                                 >  4:278440/1‑149 (MQ=255)                                                                                   gCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATggg                                                                 <  7:288583/149‑1 (MQ=255)                                                                                   gCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATggg                                                                 <  7:340727/149‑1 (MQ=255)                                                                                       acCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATGGGCacc                                                             >  7:256428/1‑147 (MQ=255)                                                                                       acCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATGGGCAAc                                                             <  3:278440/149‑1 (MQ=255)                                                                                       acCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATGGGCAAc                                                             >  8:110921/1‑149 (MQ=255)                                                                                       acCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATGGGCAAc                                                             >  8:163420/1‑149 (MQ=255)                                                                                           tGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCAGGCGGGCCTTCT‑TCGCCAGCATGGGCAACGGcg                                                         >  1:136818/1‑149 (MQ=255)                                                                                            gAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATGGGCAACGGcga                                                        >  2:361979/1‑149 (MQ=255)                                                                                             aGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATGGGCAACGGcgac                                                       <  6:112701/149‑1 (MQ=255)                                                                                             aGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATGGGCAACGGcgac                                                       <  8:252826/149‑1 (MQ=255)                                                                                                  taTCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATGGGCAACGGCGACGAAGc                                                  <  1:309435/148‑1 (MQ=255)                                                                                                        cTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCTGGCGGGCCTTCT‑TCGCCAGCATGGGCAACGGCGACGAAGCCTTCg                                             <  2:136818/148‑1 (MQ=255)                                                                                                        cTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATGGGCAACGGCGACGAAGCCTTc                                              <  4:249609/147‑1 (MQ=255)                                                                                                        cTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATGGGCAACGGCGACGAAGCCTTc                                              <  3:96938/147‑1 (MQ=255)                                                                                                        cTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCACACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCTCTCGCCAGCATGGGCAACGGCGACGAAGCCTTCg                                             <  1:50668/149‑1 (MQ=255)                                                                                                         tcgtcgATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGcc                                                                                   >  8:346106/1‑110 (MQ=255)                                                                                                         tcgtcgATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGcc                                                                                   <  7:346106/110‑1 (MQ=255)                                                                                                           gtcgATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATGGGCAACGGCGACGAAGCCTTCGCTAc                                         >  5:303982/1‑149 (MQ=255)                                                                                                            tcgATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATCCGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATTGGCACAGGCGACGAAGCCTTCGCTACg                                        >  4:59304/1‑149 (MQ=255)                                                                                                            tcgATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATGGGCAACGGCGACGAAGCCTTCGCTACg                                        >  8:275048/1‑149 (MQ=255)                                                                                                               atgatgCGTTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATGGGCAACGGCGACGAAGCCTTCGCTACGGgc                                     >  3:9274/1‑149 (MQ=255)                                                                                                               atgatgCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATGGGCAACGGCGACGAAGCCTTCGCGACGGgc                                     >  8:298323/1‑149 (MQ=255)                                                                                                                 gatgCGCTGCATCAGGCTCTTGTCCTCGATGGCGCGCACGCCATGGTAGATACGTTTGATCTTCAGCAGGTCGTGGGCTTCCGAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATGGGTAACGGCGACGAAGCCTTCGCTACGGgcg                                    <  6:342446/148‑1 (MQ=255)                                                                                                                 gatgCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATGGGCAACGGCGACGAAGCCTTCGCTACGGgcgc                                   >  6:345972/1‑149 (MQ=255)                                                                                                                   tgCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATGGGCAACGGCGACGAAGCCTTCGCTACGGGCGCgg                                 <  5:89736/149‑1 (MQ=255)                                                                                                                    gcgcTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATGGGCAACGGCGACGAAGCCTTCGCTACGGGCGCGGt                                >  1:47630/1‑149 (MQ=255)                                                                                                                     cgcTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCttct‑tc                                                                            >  3:2735/1‑104 (MQ=255)                                                                                                                     cgcTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCttct‑tc                                                                            <  4:2735/104‑1 (MQ=255)                                                                                                                     cgcTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATGGGCAACGGCGACGAAGCCTTCGCTACGGGCGCGGTc                               <  1:216728/149‑1 (MQ=255)                                                                                                                       cTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATGGGCAACGGCGACGAAGCCTTCGCTACGGGCGCGGTCGa                             <  3:167356/149‑1 (MQ=255)                                                                                                                               ggCGGTCGTCCTCGATGGCGCGCACTCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATGGGCAACGGCGACGAAGCCTTCGCTACGGGCGCGGTCGAACACCCGc                     <  4:287664/149‑1 (MQ=255)                                                                                                                                gcgcTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATGGGCAACGGCGACGAAGCCTTCGCTACGGGCGCGGTCGAACACCCGCt                    >  8:240240/1‑149 (MQ=255)                                                                                                                                gcgcTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATGGGCAACGGCGACGAAGCCTTCGCTACGGGCGCGGTCGAACACCCGCt                    >  8:224805/1‑149 (MQ=255)                                                                                                                                 cgcTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATGGGCAACGGCGACGAAGCCTTCGCTACGGGCGCGGTCGAACACCCGCTg                   <  7:275048/149‑1 (MQ=255)                                                                                                                                  gcTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGAt                                                                                                  >  8:71597/1‑70 (MQ=255)                                                                                                                                   cTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATGGGCAACGGCGACGAAGCCTTCGCTACGGGCGCGGTCGAACACCCGCTgg                  >  2:70366/1‑148 (MQ=255)                                                                                                                                      atcgaccaTGGCGTGCGCGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATGGGCAACGGCGACGAAGCCTTCGCTACGGGCGCGGTCGAACACCCGCTGGaa                >  6:315551/8‑147 (MQ=255)                                                                                                                                      atcgaccaTGGCGTGCGCGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATGGGCAACGGCGACGAAGCCTTCGCTACGGGCGCGGTCGAACACCCGCTGGaa                <  5:315551/140‑1 (MQ=255)                                                                                                                                            gATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATGGGCAACGGCGACGAAGCCTTCGCTACGGGCGCGGTCGAACACCCGCTGGAACTTGCtgg        >  2:60205/1‑149 (MQ=255)                                                                                                                                            caTGGCGTGCGCGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATGGGCAACGGCGACGAAGCCTTCGCTACGGGCGCGGTCGAACACCCGCTGGAACTTGCtgg        >  3:372594/2‑149 (MQ=255)                                                                                                                                            caTGGCGTGCGCGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGAAGTACTCGGGCGGGCCTTCT‑TCGCCAGCATCGGCAACGGCGACGAAGCCTTCGCTACGGGCGCGGTCGAACACCCGCTGGAACTTGCtgg        >  3:213027/2‑149 (MQ=255)                                                                                                                                              tGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATGGGCAACGGCGACGAAGCCTTCGCTACGGGCGCGGTCGAACACCCGCTGGAACTTGCtgggt      <  1:70366/149‑1 (MQ=255)                                                                                                                                                  gcgcACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATGGGCAACGGCGACGAAGCCTTCGCTACGGGCGCGGTCGAACACCCGCTGGAACTTGCTgggtgggt  >  1:199227/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                     CTTTCACACCACGCTCGAGCATGTCGAGGATGTTGTGCTGGCTCATATGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATGGGCAACGGCGACGAAGCCTTCGCTACGGGCGCGGTCGAACACCCGCTGGAACTTGCTGGGTGGGT  >  NC_002947/689829‑690122

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑