Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	689,978	A→G	5.4%	V220A (GTG→GCG)	PP_0591 ←	adenosine deaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	689,978	0	A	G	5.4%	276.3 / 4.2	92	V220A (GTG→GCG)	PP_0591	adenosine deaminase
Reads supporting (aligned to +/- strand):  ref base A (43/44);  new base G (3/2);  total (46/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.26e-01

TTTCACACCACGCTCGAGCATGTCGAGGATGTTGTGCTGGCTCATATGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATGGGCAACGGCGACGAAGCCTTCGCTACGGGCGCGGTCGAACACCCGCTGGAACTTGCTGGGTGGGTGGCC  >  NC_002947/689830‑690126                                                                                                                                                     |                                                                                                                                                      tttCACACCACGCTCGAGCATGTCGAGGATGTTGTGCTGGCTCATATGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCa                                                                                                                                                       >  1:136205/1‑149 (MQ=255)   tCACACCACGCTCGAGCATGTCGAGGATGTTGTGCTGGCTCATATGGTCTAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCTGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCAc                                                                                                                                                      <  1:42537/148‑1 (MQ=255)     acacCACGCTCGAGCATGTCGAGGATGTTGTGCTGGCTCATATGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGc                                                                                                                                                    >  6:372938/1‑148 (MQ=255)     acacCACGCTCGAGCATGTCGAGGATGTTGTGCTGGCTCATATGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGc                                                                                                                                                    >  8:340727/1‑148 (MQ=255)       accacGCTCGAGCATGTCGAGGATGTTGTGCTGGCTCATATGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGTGCGCGCCAt                                                                                                                                                 <  5:141696/149‑1 (MQ=255)       accacGCTCGAGCATGTCGAGGATGTTGTGCTGGCTCATATGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCAt                                                                                                                                                 >  6:101438/1‑149 (MQ=255)       accacGCTCGAGCATGTCGAGGATGTTGTGCTGGCTCATATGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCAt                                                                                                                                                 >  2:32395/1‑149 (MQ=255)         tacGCTCGAGCATGTCGAGGATGTTGTGCTGGCTCATATGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATgg                                                                                                                                               >  4:208124/2‑149 (MQ=255)         cacGCTCGAGCATGTCGAGGATGTTGTGCTGGCTCATATGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATgg                                                                                                                                               >  6:244538/1‑149 (MQ=255)                gAGCATGTCGAGGATGTTGTGCTGGCTCATATGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATAc                                                                                                                                        <  1:338890/149‑1 (MQ=255)                  gCATGTCGAGGATGTTGTGCTGGCTCATATGGTCGAACACGCAGAGATAGGTGTTGGACAGAGGGCACACAGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTAGTCCTCCATGGCGCGCACGCCATTGTCGATAAGt                                                                                                                                      >  7:234296/1‑149 (MQ=255)                   cATGTCGAGGATGTTGTGCTGGCTCATATGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGtt                                                                                                                                     <  3:351560/149‑1 (MQ=255)                    aTGTCGAGGATGTTGTGCTGGCTCATATGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGttt                                                                                                                                    <  2:40638/149‑1 (MQ=255)                      gTCGAGGATGTTGTGCTGGCTCATATGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTg                                                                                                                                   <  2:136205/148‑1 (MQ=255)                      gTCGAGGATGTTGTGCTGGCTCATATGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTAGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTg                                                                                                                                   >  3:287664/1‑148 (MQ=255)                       tCGAGGATGTTGTGCTGGCTCATATGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGa                                                                                                                                  <  3:208124/148‑1 (MQ=255)                       tCGAGGATGTTGTGCTGGCTCATATGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGATCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGa                                                                                                                                  >  6:227273/1‑148 (MQ=255)                          aGGATGTTGTGCTGGCTCATATGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCtt                                                                                                                              <  7:92063/149‑1 (MQ=255)                             aTGTTGTGCTGGCTCATATGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTcag                                                                                                                           <  7:109167/149‑1 (MQ=255)                              tgttgtGCTGGCTCATATGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTcagc                                                                                                                          <  4:72889/149‑1 (MQ=255)                               gttgtGCTGGGTCATTCGGTCGAAGACGCAGAGCTTGGTGTTGGACAGCTGGCACTCCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGGTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTcagc                                                                                                                          <  7:277219/148‑1 (MQ=255)                               gttgtGCTGGCTCATATGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGAt                                                                                                                                          <  5:59440/132‑1 (MQ=255)                               gttgtGCTGGCTCATATGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGAt                                                                                                                                          >  6:59440/1‑132 (MQ=255)                                  gtgCTGGCTCATATGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGt                                                                                                                      >  5:112701/1‑149 (MQ=255)                                     cTGGCTCATATGGTCGAACACGCAGAGCTTGGAGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTc                                                                                                                     >  3:146545/1‑147 (MQ=255)                                        gCTCATATGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAg                                                                                                                  <  3:365119/147‑1 (MQ=255)                                          tCATATGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCt                                                                                                              >  5:63836/1‑149 (MQ=255)                                             tatGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGc                                                                                                               <  3:230036/145‑1 (MQ=255)                                             tatGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGc                                                                                                               >  4:230036/1‑145 (MQ=255)                                             tatGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTcc                                                                                                           <  7:158743/149‑1 (MQ=255)                                                 gTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAg                                                                                                        <  5:227273/148‑1 (MQ=255)                                                 gTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGa                                                                                                       <  6:63836/149‑1 (MQ=255)                                                     aaCACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTa                                                                                                   >  3:127002/1‑149 (MQ=255)                                                        acGCAGAGCTTGGTGTTGGACAGCGGGCTCACCGGGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTc                                                                                                <  8:188396/149‑1 (MQ=255)                                                         cgctgttcTTGGTGTTGTTGAGCGGTCAGAGCGTTAGTGGGATCTGCTCGTCTTTGATGCGCTGCTTCAGGTGGTCGTTGTTGATGTCGCTTACGCCATTGTCTATATGTTTGATCTTCAGCAGGTCGAGGGCTTCCCTGATGTACTc                                                                                                <  8:234296/141‑1 (MQ=255)                                                          gCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCCGATGTACTcgg                                                                                              >  7:355937/1‑149 (MQ=255)                                                          gCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTcgg                                                                                              <  2:182424/149‑1 (MQ=255)                                                          gCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTcgg                                                                                              >  8:288583/1‑149 (MQ=255)                                                           cAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTcggg                                                                                             <  5:101438/149‑1 (MQ=255)                                                            agagCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCGATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTcgggc                                                                                            >  5:342446/1‑149 (MQ=255)                                                              agCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTcgggcgg                                                                                          <  2:372228/149‑1 (MQ=255)                                                                    gtgtTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCcttc                                                                                    >  1:56080/1‑149 (MQ=255)                                                                    gtgtTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCcttc                                                                                    <  2:56080/149‑1 (MQ=255)                                                                         ggACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCcttc                                                                                    >  8:178959/1‑144 (MQ=255)                                                                         ggACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCcttc                                                                                    <  7:178959/144‑1 (MQ=255)                                                                         ggACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGc                                                                              >  1:62157/1‑149 (MQ=255)                                                                          gACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCTGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGc                                                                              <  4:146545/148‑1 (MQ=255)                                                                          gACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGc                                                                              >  2:216728/1‑148 (MQ=255)                                                                                 ggCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATgg                                                                      <  5:372938/149‑1 (MQ=255)                                                                                  gCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATggg                                                                     <  7:288583/149‑1 (MQ=255)                                                                                  gCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATggg                                                                     >  4:278440/1‑149 (MQ=255)                                                                                  gCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATggg                                                                     <  7:340727/149‑1 (MQ=255)                                                                                      acCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATGGGCacc                                                                 >  7:256428/1‑147 (MQ=255)                                                                                      acCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATGGGCAAc                                                                 >  8:163420/1‑149 (MQ=255)                                                                                      acCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATGGGCAAc                                                                 >  8:110921/1‑149 (MQ=255)                                                                                      acCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATGGGCAAc                                                                 <  3:278440/149‑1 (MQ=255)                                                                                          tGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCAGGCGGGCCTTCT‑TCGCCAGCATGGGCAACGGcg                                                             >  1:136818/1‑149 (MQ=255)                                                                                           gAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATGGGCAACGGcga                                                            >  2:361979/1‑149 (MQ=255)                                                                                            aGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATGGGCAACGGcgac                                                           <  6:112701/149‑1 (MQ=255)                                                                                            aGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATGGGCAACGGcgac                                                           <  8:252826/149‑1 (MQ=255)                                                                                                 taTCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATGGGCAACGGCGACGAAGc                                                      <  1:309435/148‑1 (MQ=255)                                                                                                       cTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCTGGCGGGCCTTCT‑TCGCCAGCATGGGCAACGGCGACGAAGCCTTCg                                                 <  2:136818/148‑1 (MQ=255)                                                                                                       cTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATGGGCAACGGCGACGAAGCCTTc                                                  <  4:249609/147‑1 (MQ=255)                                                                                                       cTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATGGGCAACGGCGACGAAGCCTTc                                                  <  3:96938/147‑1 (MQ=255)                                                                                                       cTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCACACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCTCTCGCCAGCATGGGCAACGGCGACGAAGCCTTCg                                                 <  1:50668/149‑1 (MQ=255)                                                                                                        tcgtcgATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGcc                                                                                       >  8:346106/1‑110 (MQ=255)                                                                                                        tcgtcgATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGcc                                                                                       <  7:346106/110‑1 (MQ=255)                                                                                                          gtcgATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATGGGCAACGGCGACGAAGCCTTCGCTAc                                             >  5:303982/1‑149 (MQ=255)                                                                                                           tcgATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATCCGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATTGGCACAGGCGACGAAGCCTTCGCTACg                                            >  4:59304/1‑149 (MQ=255)                                                                                                           tcgATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATGGGCAACGGCGACGAAGCCTTCGCTACg                                            >  8:275048/1‑149 (MQ=255)                                                                                                              atgatgCGTTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATGGGCAACGGCGACGAAGCCTTCGCTACGGgc                                         >  3:9274/1‑149 (MQ=255)                                                                                                              atgatgCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATGGGCAACGGCGACGAAGCCTTCGCGACGGgc                                         >  8:298323/1‑149 (MQ=255)                                                                                                                gatgCGCTGCATCAGGCTCTTGTCCTCGATGGCGCGCACGCCATGGTAGATACGTTTGATCTTCAGCAGGTCGTGGGCTTCCGAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATGGGTAACGGCGACGAAGCCTTCGCTACGGgcg                                        <  6:342446/148‑1 (MQ=255)                                                                                                                gatgCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATGGGCAACGGCGACGAAGCCTTCGCTACGGgcgc                                       >  6:345972/1‑149 (MQ=255)                                                                                                                  tgCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATGGGCAACGGCGACGAAGCCTTCGCTACGGGCGCgg                                     <  5:89736/149‑1 (MQ=255)                                                                                                                   gcgcTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATGGGCAACGGCGACGAAGCCTTCGCTACGGGCGCGGt                                    >  1:47630/1‑149 (MQ=255)                                                                                                                    cgcTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCttct‑tc                                                                                >  3:2735/1‑104 (MQ=255)                                                                                                                    cgcTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCttct‑tc                                                                                <  4:2735/104‑1 (MQ=255)                                                                                                                    cgcTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATGGGCAACGGCGACGAAGCCTTCGCTACGGGCGCGGTc                                   <  1:216728/149‑1 (MQ=255)                                                                                                                      cTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATGGGCAACGGCGACGAAGCCTTCGCTACGGGCGCGGTCGa                                 <  3:167356/149‑1 (MQ=255)                                                                                                                              ggCGGTCGTCCTCGATGGCGCGCACTCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATGGGCAACGGCGACGAAGCCTTCGCTACGGGCGCGGTCGAACACCCGc                         <  4:287664/149‑1 (MQ=255)                                                                                                                               gcgcTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATGGGCAACGGCGACGAAGCCTTCGCTACGGGCGCGGTCGAACACCCGCt                        >  8:240240/1‑149 (MQ=255)                                                                                                                               gcgcTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATGGGCAACGGCGACGAAGCCTTCGCTACGGGCGCGGTCGAACACCCGCt                        >  8:224805/1‑149 (MQ=255)                                                                                                                                cgcTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATGGGCAACGGCGACGAAGCCTTCGCTACGGGCGCGGTCGAACACCCGCTg                       <  7:275048/149‑1 (MQ=255)                                                                                                                                 gcTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGAt                                                                                                      >  8:71597/1‑70 (MQ=255)                                                                                                                                  cTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATGGGCAACGGCGACGAAGCCTTCGCTACGGGCGCGGTCGAACACCCGCTgg                      >  2:70366/1‑148 (MQ=255)                                                                                                                                     atcgaccaTGGCGTGCGCGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATGGGCAACGGCGACGAAGCCTTCGCTACGGGCGCGGTCGAACACCCGCTGGaa                    >  6:315551/8‑147 (MQ=255)                                                                                                                                     atcgaccaTGGCGTGCGCGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATGGGCAACGGCGACGAAGCCTTCGCTACGGGCGCGGTCGAACACCCGCTGGaa                    <  5:315551/140‑1 (MQ=255)                                                                                                                                           gATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATGGGCAACGGCGACGAAGCCTTCGCTACGGGCGCGGTCGAACACCCGCTGGAACTTGCtgg            >  2:60205/1‑149 (MQ=255)                                                                                                                                           caTGGCGTGCGCGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATGGGCAACGGCGACGAAGCCTTCGCTACGGGCGCGGTCGAACACCCGCTGGAACTTGCtgg            >  3:372594/2‑149 (MQ=255)                                                                                                                                           caTGGCGTGCGCGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGAAGTACTCGGGCGGGCCTTCT‑TCGCCAGCATCGGCAACGGCGACGAAGCCTTCGCTACGGGCGCGGTCGAACACCCGCTGGAACTTGCtgg            >  3:213027/2‑149 (MQ=255)                                                                                                                                             tGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATGGGCAACGGCGACGAAGCCTTCGCTACGGGCGCGGTCGAACACCCGCTGGAACTTGCtgggt          <  1:70366/149‑1 (MQ=255)                                                                                                                                                 gcgcACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATGGGCAACGGCGACGAAGCCTTCGCTACGGGCGCGGTCGAACACCCGCTGGAACTTGCTgggtgggt      >  1:199227/1‑149 (MQ=255)                                                                                                                                                     aCGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATGGGCAACGGCGACGAAGCCTTCGCTACGGGCGCGGTCGAACACCCGCTGGAACTTGCTGGGTGGGTGGcc  <  2:199227/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                      TTTCACACCACGCTCGAGCATGTCGAGGATGTTGTGCTGGCTCATATGGTCGAACACGCAGAGCTTGGTGTTGGACAGCGGGCACACCGTGAGCGGGATCTGCTCGTCGATGATGCGCTGCATCAGGCGCTCGTCCTCGATGGCGCGCACGCCATGGTCGATACGTTTGATCTTCAGCAGGTCGAGGGCTTCCCAGATGTACTCGGGCGGGCCTTCT‑TCGCCAGCATGGGCAACGGCGACGAAGCCTTCGCTACGGGCGCGGTCGAACACCCGCTGGAACTTGCTGGGTGGGTGGCC  >  NC_002947/689830‑690126

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑