Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	160,310	C→T	5.0%	E27K (GAG→AAG)	PP_0149 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	160,310	0	C	T	5.0%	304.4 / 3.5	99	E27K (GAG→AAG)	PP_0149	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (48/46);  new base T (2/3);  total (50/49)
Fisher's exact test for biased strand distribution p-value = 6.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.57e-01

CGTCGGCAGGCATGTTGTCGCTCCAGCTGACCCGCACGCGGCGGTCCAGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAG  >  NC_002947/160163‑160456                                                                                                                                                    |                                                                                                                                                    cGTCGGCAGGCATGTTGTCGCTCCAGCTGACCCGCACGCGGCGGTCCAGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCa                                                                                                                                                    <  5:249408/149‑1 (MQ=255)   tCGGCAGGCATGTTGTCGCTCCAGCTGACCCGCACGCGGCGGTCCAGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAg                                                                                                                                                  <  3:199986/149‑1 (MQ=255)         ggcaTGTTGTCGCTCCAGCTGACCCGCACGCGGCGGTCCAGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTTGAGCTGCAg                                                                                                                                            <  2:265208/149‑1 (MQ=255)          gcaTGTTGTCGCTCCAGCTGACCCGCACGCGGCGGTCCAGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAgg                                                                                                                                           >  7:168724/1‑149 (MQ=255)           caTGTTGTCGGTCCAGCTGAACCGCACGCGGCGGTGCAGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTTGAGCTGCAggc                                                                                                                                          <  3:122036/149‑2 (MQ=255)           caTGTTGTCGCTCCAGCTGACCCGCACGCGGCGGTCCAGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTTGAGCTGCAggc                                                                                                                                          <  6:199092/149‑2 (MQ=255)            aTGTTGTCGCTCCAGCTGACCCGCACGCGGCGGTCCAGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTc                                                                                                                                         <  7:312235/149‑1 (MQ=255)            aTGTTGTCGCTCCAGCTGACCCGCACGCGGCGGTCCAGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTc                                                                                                                                         >  6:344142/1‑149 (MQ=255)                tgtCGCTCCAGCTGACCCGCACGCGGCGGTCCAGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGG‑c                                                                                                                                     >  1:332908/1‑148 (MQ=255)                 gtCGCTCCAGCTGACCCGCACGCGGCGGTCCAGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCg                                                                                                                                        >  3:216583/1‑145 (MQ=255)                 gtCGCTCCAGCTGACCCGCACGCGGCGGTCCAGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCg                                                                                                                                        <  4:216583/145‑1 (MQ=255)                       ccAGCTGACCCGCACGCGGCGGTCCAGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGG‑CCAAggcgg                                                                                                                             <  6:24089/149‑1 (MQ=255)                        cAGCTGACCCGCACGCGGCGGTCCAGCTGTTGCTTGAAGCGGGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGG‑CCAAggcgg                                                                                                                             <  8:233748/148‑1 (MQ=255)                        cAGCTGACCCGCACGCGGCGGTCCAGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGG‑CCAAggcggg                                                                                                                            >  8:226994/1‑149 (MQ=255)                         aGCTGACCCGCACGCGGCGGTCCAGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGG‑CCAAggcgggc                                                                                                                           <  4:240654/149‑1 (MQ=255)                              aCCCGCACGCGGCGGTCCAGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTGcc                                                                                                                      >  7:346216/1‑149 (MQ=255)                              aCCCGCACGCGGCGGTCCAGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTGcc                                                                                                                      >  6:91002/1‑149 (MQ=255)                                ccGCACGCGGCGGTCCAGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTGcc                                                                                                                      >  7:11042/1‑147 (MQ=255)                                ccGCACGCGGCGGTCCAGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTGCCGa                                                                                                                    <  5:91002/149‑1 (MQ=255)                                 cGCACGCGGCGGTCCAGCTGTTGCTTGAAGCGCGGAGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTGCCGag                                                                                                                   >  8:279739/1‑149 (MQ=255)                                  gCACGCGGCGGTCCAGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTGCCGagc                                                                                                                  <  3:186719/149‑1 (MQ=255)                                  gCACGCGGCGGTCCAGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTGCCGag                                                                                                                   >  7:10803/1‑148 (MQ=255)                                         gcggTCCAGCTGTTGCTTGAAGCGCGGTGGAAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTGCCGAGCAGCGTCa                                                                                                           <  2:120805/149‑1 (MQ=255)                                          cggTCCAGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGGCGG‑CCAAGGCGGGCGTGCCGAGCAGCGTCAg                                                                                                          >  6:242067/1‑149 (MQ=255)                                           ggTCCAGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTGCCGAGCAGCGTCAgg                                                                                                         <  4:7570/149‑1 (MQ=255)                                             tCCAGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTGCCGAGCAGCGTCAGGgc                                                                                                       >  1:179310/1‑149 (MQ=255)                                               cAGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTGCCGAGCAGCGTCAGGgcgc                                                                                                     >  8:208190/1‑149 (MQ=255)                                                aGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTGCCGAGCAGCGTCAGGgcg                                                                                                      >  7:24693/1‑147 (MQ=255)                                                aGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTGCCGAGCAGCGTCAGGgcg                                                                                                      >  6:165568/1‑147 (MQ=255)                                                 gCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAg                                                                                                   <  2:332908/149‑1 (MQ=255)                                                 gCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCACAg                                                                                                   <  8:346216/149‑1 (MQ=255)                                                  cTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGGTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGc                                                                                                  >  3:201578/1‑149 (MQ=255)                                                   tGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGcc                                                                                                 <  5:95833/149‑1 (MQ=255)                                                     ttGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGcc                                                                                                 <  1:258286/148‑1 (MQ=255)                                                     ttGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGcc                                                                                                 >  6:85788/1‑147 (MQ=255)                                                     ttGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGcc                                                                                                 >  7:193622/1‑147 (MQ=255)                                                     tggCTTGACGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGGTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCaa                                                                                               <  6:62085/147‑1 (MQ=255)                                                       gCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAag                                                                                              >  4:232624/1‑148 (MQ=255)                                                       gCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAag                                                                                              >  7:205294/1‑148 (MQ=255)                                                        cTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAagc                                                                                             <  8:245734/148‑1 (MQ=255)                                                         ttGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCa                                                                                                                                             >  6:343828/1‑100 (MQ=255)                                                         ttGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCa                                                                                                                                             <  5:343828/100‑1 (MQ=255)                                                           gAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAagcagc                                                                                          >  7:321146/1‑148 (MQ=255)                                                            aaGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAagcagc                                                                                          >  7:1975/1‑147 (MQ=255)                                                            aaGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAagcagc                                                                                          >  3:360776/1‑147 (MQ=255)                                                             aGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAg                                                                                       <  7:338070/149‑1 (MQ=255)                                                             aGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAg                                                                                       <  7:342939/149‑1 (MQ=255)                                                              gcgcGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCGGCAGGTCGG‑CCAAGGCGGGCGTGCCGCGCAGCGTCATGGCGCATCCAAGCAGCCAgg                                                                                      >  7:309463/1‑149 (MQ=255)                                                                 cgGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGgcac                                                                                   <  5:324693/149‑1 (MQ=255)                                                                  ggTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGgca                                                                                    <  7:279739/147‑1 (MQ=255)                                                                     ggCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGgcacgcac                                                                               <  8:24693/149‑1 (MQ=255)                                                                     ggCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGGCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGgcacgcac                                                                               <  3:41414/149‑1 (MQ=255)                                                                         gCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACgcgc                                                                           <  8:168724/149‑1 (MQ=255)                                                                             gCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCtt                                                                         <  3:134496/147‑1 (MQ=255)                                                                              cccATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTcac                                                                      <  5:85788/149‑1 (MQ=255)                                                                              cccATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTca                                                                       >  2:369672/1‑148 (MQ=255)                                                                               ccATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTcaca                                                                     <  7:208190/149‑1 (MQ=255)                                                                               ccATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTca                                                                       >  1:15987/1‑147 (MQ=255)                                                                                cATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAg                                                                    >  3:189348/1‑149 (MQ=255)                                                                                   ggCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTgg                                                                 >  4:359114/1‑148 (MQ=255)                                                                                      cTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTGCCGAGCAGCGACAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAg                                                              >  6:191943/1‑149 (MQ=255)                                                                                           cgAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATgg                                                         >  8:324605/1‑149 (MQ=255)                                                                                            gAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGc                                                        <  7:226994/149‑1 (MQ=255)                                                                                            gAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGGCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATgg                                                         <  2:134484/148‑1 (MQ=255)                                                                                                   gCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGc                                                  >  4:239893/1‑148 (MQ=255)                                                                                                    cTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTc                                                     >  7:175530/1‑144 (MQ=255)                                                                                                    cTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTc                                                     <  8:175530/144‑1 (MQ=255)                                                                                                         gAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATAcc                                           <  4:360776/149‑1 (MQ=255)                                                                                                            gCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCaa                                               >  6:81412/1‑142 (MQ=255)                                                                                                            gCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCaa                                               <  5:81412/142‑1 (MQ=255)                                                                                                              tttAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTTCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGcacgcacg                                                                              >  7:173414/1‑109 (MQ=255)                                                                                                              tttAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGcacgcacg                                                                              <  8:173414/109‑1 (MQ=255)                                                                                                              tttAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTc                                      >  8:316068/1‑149 (MQ=255)                                                                                                                  gTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCt                                   <  5:242067/148‑1 (MQ=255)                                                                                                                   tttGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTgg                                 <  3:232624/149‑1 (MQ=255)                                                                                                                       ttgtGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGcgcc                             >  5:29710/1‑149 (MQ=255)                                                                                                                         gtgGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGcgccgc                           >  4:91136/1‑149 (MQ=255)                                                                                                                           gggTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCGGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCcgcgc                         >  1:250065/1‑149 (MQ=255)                                                                                                                            ggTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCcgcgc                         <  4:189112/148‑1 (MQ=255)                                                                                                                             gTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCgcgcgc                       >  3:45078/1‑149 (MQ=255)                                                                                                                                  ggCCGGACGCCTGCAGCTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCg                  <  8:1975/149‑1 (MQ=255)                                                                                                                                   gCCGGACGCCTGCAGCTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCgg                 <  7:324605/149‑1 (MQ=255)                                                                                                                                   gCCGGACGCCTGCAGCTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCgg                 >  1:25743/1‑149 (MQ=255)                                                                                                                                    ccGGACGCCTGCAGCTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTGCCGAGCAGCGTCTGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCgg                 >  3:222455/1‑148 (MQ=255)                                                                                                                                     cGGCCGCCTGCAGCTCAAGCTGCAGGGCGG‑CCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTa               <  2:269555/149‑1 (MQ=255)                                                                                                                                      ggACGCCTGCAGCTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTAc              >  5:363973/1‑149 (MQ=255)                                                                                                                                        cgaccTGCAGCTTGAGCTGCAGGTCGG‑CCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCCAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTActc            >  1:378245/4‑149 (MQ=255)                                                                                                                                        aCGCCTGCAGCTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTActc            <  8:11042/149‑1 (MQ=255)                                                                                                                                            cTGCAGCTTGAGCTGCAGGTCGG‑CCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTActcgctc        >  2:228232/1‑149 (MQ=255)                                                                                                                                            cTGCAGCTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCaa                                                                                               >  7:215626/1‑62 (MQ=255)                                                                                                                                            cTGCAGCTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCaa                                                                                               <  8:215626/62‑1 (MQ=255)                                                                                                                                            cTGCAGCTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTActcgctc        >  5:185754/1‑149 (MQ=255)                                                                                                                                            cTGCAGCTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTActcgctc        <  4:201578/149‑1 (MQ=255)                                                                                                                                            cTGCAGCTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTActcgctc        <  8:321146/149‑1 (MQ=255)                                                                                                                                            cTGCAGCTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTActcgc          >  6:284988/1‑147 (MQ=255)                                                                                                                                            cTGCAGCTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTActcgc          <  5:284988/147‑1 (MQ=255)                                                                                                                                             tGCAGCTCAAGCTGCAGGCCGG‑CCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTActcgctcg       >  3:224505/1‑149 (MQ=255)                                                                                                                                                  cTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAg  >  3:308582/1‑149 (MQ=255)                                                                                                                                                  cTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAg  <  6:29710/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                    CGTCGGCAGGCATGTTGTCGCTCCAGCTGACCCGCACGCGGCGGTCCAGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGG‑CCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAG  >  NC_002947/160163‑160456

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑