Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 704,278 G→T 8.1% R124L (CGG→CTG)  PP_5745 → paraquat‑inducible protein A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947704,2780GT 8.1% 206.1 / 6.2 72R124L (CGG→CTG) PP_5745paraquat‑inducible protein A
Reads supporting (aligned to +/- strand):  ref base G (32/34);  new base T (3/3);  total (35/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.95e-01

GTAGCCCGGACACCATCATGTCCGGTGTCATCACCCTGCTCAAGCACGGCATGGTGCCCATTGCGGCTGTGGTGTTCATTGCCAGTATCCTGGTCCCCACGTTCAAGCTGGTGGGCATCGGCTTGCTGTTGTACTCCGTTCAGCGGCGGCAGCCGCTTTCGGCCCGGCAGCGGATATTGA‑‑TGTACCGCTTCATCGAATTCATTGGGCGCTGGTCCATGCTGGACATCTTCGTCATTGCCATCCTGGTGGCAGTGGTGAATTTTGGCCGAATAGCCAGTGTCGAAGCCAACCTGG  >  NC_002947/704131‑704424
                                                                                                                                                   |                                                                                                                                                    
gTAGCCCGGACACCATCATGTCCGGTGTCATCACCCTGCTCAAGCACGGCATGGTGCCCATTGCGGCTGTGGTGTTCATTGCCAGTATCCTGGTCCCCACGTTCAAGCTGGTGGGCATCGGCTTGCTGTTGTACTCCGTTCAGcggcgg                                                                                                                                                     <  6:352859/149‑1 (MQ=255)
    accGGACATGATGGTGTCCGGTGTCATCACCCTGCTCAAGCACGGCATGGTGCCCATTGCGGCTGTGGTGTTCATTGCCAGTATCCTGGTCCCCACGTTCAAGCTGGTGGGCATCGGCTTGCTGTTGTACTCCGTTCAGCGGCGGCAGc                                                                                                                                                 >  7:297234/2‑149 (MQ=255)
     ccGGACACCATCATGTCCGGTGTCATCACCCTGCTCAAGCACGGCATGGTGCCCATTGCGGCTGTGGTGTTCATTGCCAGTATCCTGGTCCCCACGTTCAAGCTGGTGGGCATCGGCTTGCTGTTGTACTCCGTTCAGCGGCGGCAGcc                                                                                                                                                >  1:49297/1‑149 (MQ=255)
             catcatGTCCGGTGTCATCACCCTGCTCAAGCACGGCATGGTGCCCATTGCGGCTGTGGTGTTCATTGCCAGTATCCTGGTCCCCACGTTCAAGCTGGTGGGCATCGGCTTGCTGTTGTACTCCGTTCAGCGGCTGCCGCCGCtgaac                                                                                                                                         >  1:120506/1‑144 (MQ=255)
             catcatGTCCGGTGTCATCACCCTGCTCAAGCACGGCATGGTGCCCATTGCGGCTGTGGTGTTCATTGCCAGTATCCTGGTCCCCACGTTCAAGCTGGTGGGCATCGGCTTGCTGTTGTACTCCGTTCAGCGGCTGCCGCCGCtgaac                                                                                                                                         <  2:120506/148‑5 (MQ=255)
             catcatGTCCGGTGTCATCACCCTGCTCAAGCACGGCATGGTGCCCATTGCGGCTGTGGTGTTCATTGCCAGTATCCTGGTCCCCACGTTCAAGCTGGTGGGCATCGGCTTGCTGTTGTACTCCGTTCAGCGGCGGCAGCCGCTTTc                                                                                                                                          <  2:342247/147‑1 (MQ=255)
             catcatGTCCGGTGTCATCACCCTGCTCAAGCACGGCATGGTGCCCATTGCGGCTGTGGTGTTCATTGCCAGTATCCTGGTCCCCACGTTCAAGCTGGTGGGCATCGGCTTGCTGTTGTACTCCGTTCAGCGGCGGCAGCCGCTTTc                                                                                                                                          >  1:342247/1‑147 (MQ=255)
             catcatGTCCGGTGTCATCACCCTGCTCAAGCACGGCATGGTGCCCATTGCGGCTGTGGTGTTCATTGCCAGTATCCTGGTCCCCACGTTCAAGCTGGTGGGCATCGGCTTGCTGTTGTACTCCGTTCAGCGGCGGCAGCCGCTTTCgg                                                                                                                                        >  2:328810/1‑149 (MQ=255)
              atggtGTCCGGTGTCATCACCCTGCTCAAGCACGGCATGGTGCCCATTGCGGCTGTGGTGTTCATTGCCAGTATCCTGGTCCCCACGTTCAAGCTGGTGGGCATCGGCTTGCTGTTGTACTCCGTTCAGCGGCGGCAGCCGCTTTCGGc                                                                                                                                       <  8:297234/145‑1 (MQ=255)
              atcatGTCCGGTGTCATCACCCTGCTCAAGCACGGCATGGTGCCCATTGCGGCTGTGGTGTTCATTGCCAGTATCCTGGTCCCCACGTTCAAGCTGGTGGGCATCGGCTTGCTGTTGTACTCCGTTCAGCGGCTGCCGCCGCtgaacgg                                                                                                                                       <  7:334681/149‑7 (MQ=255)
               tcatGTCCGGTGTCATCACCCTGCTCAAGCACGGCATGGTGCCCATTGCGGCTGTGGTGTTCATTGCCAGTATCCTGGTCCCCACGTTCAAGCTGGTGGGCATCGGCTTGCTGTTGTACTCCGTTCAGCGGCGGCAGCCGCTTTCGGcc                                                                                                                                      >  8:2655/1‑149 (MQ=255)
               tcatGTCCGGTGTCATCACCCTGCTCAAGCACGGCATGGTGCCCATTGCGGCTGTGGTGTTCATTGCCAGTATCCTGGTCCCCACGTTCAAGCTGGTGGGCATCGGCTTGCTGTTGTACTCCGTTCAGCGGCGGCAGCCGCTTTCGGcc                                                                                                                                      >  5:309501/1‑149 (MQ=255)
                catGTCCGGTGTCATCACCCTGCTCAAGCACGGCATGGTGCCCATTGCGGCTGTGGTGTTCATTGCCAGTATCCTGGTCCCCACGTTCAAGCTGGTGGGCATCGGCTTGCTGTTGTACTCCGTTCAGCGGCTGCCGCCGCtgaacgga                                                                                                                                      <  6:125953/148‑8 (MQ=255)
                 atGTCCGGTGTCATCACCCTGCTCAAGCACGGCATGGTGCCCATTGCGGCTGTGGTGTTCATTGCCAGTATCCTGGTCCCCACGTTCAAGCTGGTGGGCATCGGCTTGCTGTTGTACTCCGTTCAGCGGCGGGAGCCGCTTTCGGccc                                                                                                                                     <  3:152797/148‑1 (MQ=255)
                 atGTCCGGTGTCATCACCCTGCTCAAGCACGGCATGGTGCCCATTGCGGCTGTGGTGTTCATTGCCAGTATCCTGGTCCCCACGTTCAAGCTGGTGGGCATCGGCTTGCTGTTGTACTCCGTTCAGCGGCGGCAGCCGCTTTCGGCCCg                                                                                                                                    <  2:49297/149‑1 (MQ=255)
                  tGTCCGGTGTCATCACCCTGCTCAAGCACGGCATGGTGCCCATTGCGGCTGTGGTGTTCATTGCCAGTATCCTGGTCCCCACGTTCAAGCTGGTGGGCATCGGCTTGCTGTTGTACTCCGTTCAGCGGCGGCAGCCGCTTTCGGCCCgg                                                                                                                                   <  4:127072/149‑1 (MQ=255)
                      cGGTGTCATCACCCTGCTCAAGCACGGCATGGTGCCCATTGCGGCTGTGGTGTTCATTGCCAGTATCCTGGTCCCCACGTTCAAGCTGGTGGGCATCGGCTTGCTGTTGTACTCCGTTCAGCGGCGGCAGCCGCTTTCGGCCCGGCAGc                                                                                                                               >  1:120498/1‑149 (MQ=255)
                                aCCCTGCTCAAGCACGGCATGGTGCCCATTGCGGCTGTGGTGTTCATTGCCAGTATCCTGGTCCCCAAGTTCAAGCTGGTCGGCATCGGCTTGCTGTTGTAATCCGTTCAGCGGCGGCAGCAGCTTTCGGACAGGCAGCGGATATTGA‑‑t                                                                                                                   >  8:316959/1‑149 (MQ=255)
                                 cccTGCTCAAGCACGGCATGGTGCCCATTGCGGCTGTGGTGTTCATTGCCAGTATCCTGGTCCCCACGTTCAAGCTGGTGGGCATCGGCTTGCTGTTGTACTCCGTTCAGCGGCGGCAGCCGCTTTCGGCCCGGCAGCGGATATTGA‑‑Tg                                                                                                                  <  7:295015/149‑1 (MQ=255)
                                   cttcTCAAGCACGGCATGGTGCCCATTGCGGCTGTGGTGTTCATTGCCAGTATCCTTGTCCCCACGTTCAAGCTGGTGGGCATCGGCTTGCTGTTGTACTCCGTTCAGCGCCGGCAGCCGCTTTCGGACCGGCAGCGGATATTGA‑‑TGt                                                                                                                 >  6:159544/4‑148 (MQ=255)
                                      cTCAAGCACGGCATGGTGCCCATTGCGGCTGTGGTGTTCATTGCCAGTATCCTGGTCCCCACGTTCAAGCTGGTGGGCATCGGCTTGCTGTTGTACTCCGTTCAGCGGCGGCAGCCGCTTTCGGCCCGGCAGCGGATATTGA‑‑TGTAcc                                                                                                              <  1:126323/148‑1 (MQ=255)
                                      cTCAAGCACGGCATGGTGCCCATTGCGGCTGTGGTGTTCATTGCCAGTATCCTGGTCCCCACGGTCAAGCTGGTGGGCATCGGCTTGCTGTTGTCCTCCGTTCAGCGGCGGCCGCCGGTTTCGGCCGGGCAGCGGCTATTGA‑‑TGTACCg                                                                                                             >  8:175604/1‑149 (MQ=255)
                                       tCAAGCACGGCATGGTGCCCATTGCGGCTGTGGTGTTCATTGCCAGTATCCTGGTCCCCACGTTCAAGCTGGTGGGCATCGGCTTGCTGTTGTACTCCGTTCAGCGGCGGCAGCCGCTTTCGGCCCGGCAGCGGATATTGA‑‑TGTCCCGc                                                                                                            >  7:334881/1‑149 (MQ=255)
                                       tCAAGCACGGCATGGTGCCCATTGCGGCTGTGGTGTTCATTGCCAGTATCCTGGTCCCCACGTTCAAGCTGGTGGGCATCGGCTTGCTGTTGTACTCCGTTCAGCGGCGGCAGCCGCTTTCGGCCCGGCAGCGGATATTGA‑‑TGTACCGc                                                                                                            >  4:337332/1‑149 (MQ=255)
                                        cAAGCACGGCATGGTGCCCATTGCGGCTGTGGTGTTCATTGCCAGTATCCTGGTCCCCACGTTCAAGCTGGTGGGCATCGGCTTGCTGTTGTACTCCGTTCAGCGGCGGCAGCCGCTTTCGGCCCGGCAGCGGATATTGA‑‑TGTACCGCt                                                                                                           >  2:153641/1‑149 (MQ=255)
                                        cAAGCACGGCATGGTGCCCATTGCGGCTGTGGTGTTCATTGCCAGTATCCTGGTCCCCACGTTCAAGATGGTGGGCATCGGCTTGCTGTTGTACTCCGTTCAGCGGCGGCAGCCGCTTTCGGCCAGGCAGCGGATATTGA‑‑TGTACCGCt                                                                                                           >  8:147273/1‑149 (MQ=255)
                                            cACGGCATGGTGCCCATTGCGGCTGTGGTGTTCATTGCCAGTATCCTGGTCCCCACGTTCAAGCTGGTGGGCATCGGCTTGCTGTTGTACTCCGTTCAGCGGCGGCAGCCGCTTTCGGCCCGGCAGCGGATATTGA‑‑TGTACCGCTTCAt                                                                                                       >  5:165525/1‑149 (MQ=255)
                                                gCATGGTGCCCATTGCGGCTGTGGTGTTCATTGCCAGTATCCTGGTCCCCACGTTCAAGCTGGTGGGCATCGGCTTGCTGTTGTACTCCGTTCAGCGGCGGCAGCCGCTTTCGGCCCGGCAGCGGATATTGA‑‑TGTACCGCTTCATCGaa                                                                                                   <  1:4995/149‑1 (MQ=255)
                                                   tGGTGCCCATTGCGGCTGTGGTGTTCATTGCCAGTATCCTGGTCCCCACGTTCAAGCTGGTGGGCATCGGCTTGCTGTTGTACTCCGTTCAGCGGCGGCAGCCGCTTTCGGCCCGGCAGCGGATATTGA‑‑TGTACCGCTTCATCGAATTc                                                                                                <  4:297416/149‑1 (MQ=255)
                                                               cGGCTGTGGTGTTCATTGCCAGTATCCTGGTCCCCACGTTCAAGCTGGTGGGCATCGGCTTGCTGTTGTACTCCGTTCAGCGGCGGCAGCCGCTTTCGGCCCGGCAGCGGATATTGA‑‑TGTACCGCTTCATCGAATTCATTGGGCGCTg                                                                                     <  2:120498/148‑1 (MQ=255)
                                                               cGGCTGTGGTGTTCATTGCCAGTATCCTGGTCCCCACGTTCAAGCTGGTGGGCATCGGCTTGCTGTTGTACTCCGTTCAGCGGCGGCAGCCGCTTTCGGCCCGGCAGCGGATATTGA‑‑TGTACCGCTTCATCGAATTCATTGGGCGCTg                                                                                     <  4:261539/148‑1 (MQ=255)
                                                                   tgtgGTGTTCATTGCCAGTATCCTGGTCCCCACGTTCAAGCTGGTGGGCATCGGCTTGCTGTTGTACTCCGTTCAGCGGCGGCAGCCGCTTTCGGCCCGGCAGCGGATATTGA‑‑TGTACCGc                                                                                                            >  8:210827/1‑121 (MQ=255)
                                                                   tgtgGTGTTCATTGCCAGTATCCTGGTCCCCACGTTCAAGCTGGTGGGCATCGGCTTGCTGTTGTACTCCGTTCAGCGGCGGCAGCCGCTTTCGGCCCGGCAGCGGATATTGA‑‑TGTACCGc                                                                                                            <  7:210827/121‑1 (MQ=255)
                                                                    gtggtgTTCATTGCCAGTATCCTGGTCCCCACGTTCAAGCTGGTGGGCATCGGCTTGCTGTTGTACTCCGTTCAGCGGCGGCAGCCGCTTTCGGCCCGGCAGCGGATATTGA‑‑TGTACCGCTTCATCGAATTCATTGGGCGCTGGTCCAt                                                                               <  2:263192/149‑1 (MQ=255)
                                                                         gtTCATTGCCAGTATCCTGGTCCCCACGTTCAAGCTGGTGGGCATCGGCTTGCTGTTGTACTCCGTTCAGCGGCGGCAGCCGCTTTCGGCCCGGCAGCGGATATTGA‑‑TGTACCGCTTCATCGAATTCATTGGGCGCTGGTCCATGCTg                                                                           >  8:195257/1‑148 (MQ=255)
                                                                          ttCATTGCCAGTATCCTGGTCCCCACGTTCAAGCTGGTGGGCATCGGCTTGCTGTTGTACTCCGTTCAGCGGCGGCAGCCGCTTTCGGCCCGGCAGCGGATATTGA‑‑TGTACCGCTTCATCGAATTCATTGGGCGCTTGTCCATGCTgg                                                                          <  7:2655/148‑1 (MQ=255)
                                                                            cATTGCCAGTATACTGGTCCCCAAGTTCAAGCTGGTGGGCATCGGCTTTCTGTTTTAATCCGTTCATCGGCGGCAGCCGCTTTCGGCCCGGCAGCGGATTTTGA‑‑TGTACCGCTTCATCGAATTCATTGGGCGCTGGTCCATGCTTGAc                                                                        <  7:161500/148‑1 (MQ=255)
                                                                             aTTGCCAGTATCCTGGTCCCCACGTTCAAGCTGGTGGGCATCGGCTTGCTGTTGTACTCCGTTCAGCGGCGGCAGCCGCTTTCGGCCCGGCAGCGGATATTGA‑‑TGTACCGCTTCATCGAATTCATTGGGCGCTGGTCCATGCTGGACAt                                                                      >  8:187880/1‑149 (MQ=255)
                                                                             aTTGCCAGAATCCTGGTCCCCACGTTCAAGCTGGTGGGCATCGGCTTGCTGTTGTACTCCGTTCAGCGGCGGCAGCCGCTTTCGGCCCGGCAGCGGATATTGA‑‑TGTACCGCTTCAACGAATTCATTGGGCGCTGGTCCAAGCTGGACAt                                                                      >  4:307571/1‑149 (MQ=255)
                                                                                gCCAGTATCCTGGTCCCCACGTTCAAGCTGGTGGGCATCGGCTTGCTGTTGTACTCCGTTCAGCGGCGGCAGCCGCTTTCGGCCCGGCAGCGGATATTGATCTGGGTGGCTTCATCGAATTCATTGGGCGCTGGTCCATGATGGACATc                                                                     >  1:316268/1‑149 (MQ=255)
                                                                                 ccAGTATCCTGGTCCCCACGTTCAAGCTGGTGGGCATCGGCTTGCTGTTGTACTCCGTTCAGCGGCGGCAGCCGCTTTCGGCCCGGCAGCGGATATTGT‑‑TGTACCGCTTCATCGAATTCATTGGGCGCTGGTCCATGCTGGACATCTTc                                                                  <  1:284441/149‑1 (MQ=255)
                                                                                  cAGTATCCTGGTCCCCACGTTCAAGCTGGTGGGCATCGGCTTGCTGTTGTACTCCGTTCAGCGGCGGCAGCCGCTTTCGGCCCGGCAGCGGATATTGA‑‑TGTACCGCTTCATCGAATTCAtt                                                                                             <  4:319052/121‑1 (MQ=255)
                                                                                  cAGTATCCTGGTCCCCACGTTCAAGCTGGTGGGCATCGGCTTGCTGTTGTACTCCGTTCAGCGGCGGCAGCCGCTTTCGGCCCGGCAGCGGATATTGA‑‑TGTACCGCTTCATCGAATTCAtt                                                                                             >  3:319052/1‑121 (MQ=255)
                                                                                  cAGTATCCTGGTCCCCACGTTCAAGCTGGTGGGCATCGGCTTGCTGTTGTACTCCGTTCAGCGGCGGCAGCCGCTTTCGGCCCGGCAGCGGATATTGA‑‑TGTACCGCTTCATCGAATTCATTGGGCGCTGGTCCATGCTGGACATCTTCg                                                                 >  6:138679/1‑149 (MQ=255)
                                                                                  cAGTATCCTGGTCCCCACGATCAAGCTCGTGGGCATCGTCTTGCTGTTGTACTACGTTCAGCGGCTGCAGCCGCATTCGGCCCGGCAGCGGATAATGA‑‑TGTAACGCTTCATCGAATTGATTGGGCGCTGGACCTTGCTGGACAtaatcg                                                                 >  4:278569/1‑144 (MQ=255)
                                                                                       tCCTGGTCCCCACGTTCAAGCTGGTGGGCATCGGCTTGCTGTTGTACTCCGTTCAGCGGCGGCAGCCGCTTTCGGCCCGGCAGCGGATATTGA‑‑TGTACCGCTTCATCGAATTCATTGGGCGCTGGTCCATGCTGGACATCTTCGTCAtt                                                            <  7:195257/149‑1 (MQ=255)
                                                                                         cTGGTCCCCACGTTCAAGCTGGTGGGCATCGGCTTGCTGTTGTACTCCGTTCAGCGGCGGCAGCCGCTTTCGGCCCGGCAGCGGTTATTGA‑‑TGTACCGCTTCATCGAATTCATTGGGCGCTGGTCCATGCTGGACATCTTCGTCATTg                                                           <  1:328810/148‑1 (MQ=255)
                                                                                          tGGTCCCCACGTTCAAGCTGGTGGGCATCGGCTTGCTGTTGTACTCCGTTCAGCGGCGGCAGCCGCTTTCGGCCCGGCAGCGGATATTGA‑‑TGTACCGCTTCATCGAATTCATTGGGCGCTTGTCCATGCTGGACATCTTCGTCATTGc                                                          >  2:74536/1‑148 (MQ=255)
                                                                                                ccACGTTCAAGCTGGTGGGCATCGGCTTGCTGTTGTACTCCGTTCAGCGGCGGCAGCCGCTTTCGGCCCGGCAGCGGATATTGA‑‑TGTACCGCTTCATCGAATTCATTGGGCGCTGGTCCATGCTGGACATCTTCGTCATTGCCATCCtg                                                   >  5:48575/1‑149 (MQ=255)
                                                                                                  aCGTTCAAGCTGGTGGGCATCGGCTTGCTGTTGTACTCCGTTCAGCGGCGGCAGCCGCTTTCGGCCCGGCAGCGGATATTGA‑‑TGTACCGCTTCATCGAATTCATTGGGCGCTGGTCCATGCTGGACATCTTCGTCATTGCCATCCtggt                                                 <  6:48575/149‑1 (MQ=255)
                                                                                                    gTTCAAGCTGGTGGGCATCGGCTTGCTGTTGTACTCCGTTCAGCGGCGGCAGCCGCTTTCGGCCCGGCAGCGGATATTGA‑‑TGTACCGCTTCATCGAATTCATTGGGCGCTGGTCCATGCTGGACATCTTCGTCATTGCCATCCtggtgg                                               >  3:169771/1‑149 (MQ=255)
                                                                                                       cAAGCTGGTGCGCATCGGCTTGCTGTTGTACTCCGTTCAGCGGCGGCAGCCGCTTTCGGCCCGGCAGCGGATATTGA‑‑TGTAACGCTTCATCGAATTCATTGGGCGCTGGTCAATGCTGGACATCTTCGTCAATGCCATCCAGGAGCCAg                                            >  4:28027/1‑149 (MQ=255)
                                                                                                        aaGCTGGTGGGCATCGGCTTGCTGTTGTACTCCGTTCAGCGGCGGCAGCCGCTTTCGGCCCGGCAGCGGATATTGA‑‑TGTACCGCTTCATCGAATTCATTGGGCGCTGGTCCATGCTGGACATCTTCGTCATTGCCATCCTGGTGGCAgt                                           <  5:138679/149‑1 (MQ=255)
                                                                                                         aGCTGGTGGGCATCGGCTTGCTGTTGTACTCCGTTCAGCGGCGGCAGCCGCTTTCGGCCCGGCAGCGGATATTGA‑‑TGTACCGCTTCATCGAATTCATTGGGCGCTTGTCCATGCTGGACATCTTCGTCATTGCCATCCTGGTGGCAgtg                                          <  1:74536/149‑1 (MQ=255)
                                                                                                         aGCTGGTGGGCATCGGCTTGCTGTTGTACTCCGTTCAGCGGCGGCAGCCGCTTTCGGCCCGGCAGCGGATATTGA‑‑TGTACCGCTTCATCGAATTCATTGGGCGCTGGTCCATGCTGGACATCTTCGTCATTGCCATCCTGGTGGCAgtg                                          <  7:147273/149‑1 (MQ=255)
                                                                                                              gtggGCATCGGCTTGCTGTTGTACTCCGTTCAGCGGCGGCAGCCGCTTTCGGCCCGGCAGCGGATATTGA‑‑TGTACCGCTTCATCGAATTCATTGGGCGCTGGTCCATGCTGGACATCTTCGTCATTGCCATCCTGGTGGCAGTGGTGaa                                     >  6:228234/1‑149 (MQ=255)
                                                                                                                   cATCGGCTTGCTGTTGTACTCCGTTCAGCGGCGGCAGCCGCTTTCGGCCCGGCAGCGGATATTGA‑‑TGTACCGCTTCATCGAATTCATTGGGCGCTGGTCCATGCTGGACATCTTCGTCATTGCCATCCTGGTGGCAGTGGTGAATTTTg                                <  7:175604/149‑1 (MQ=255)
                                                                                                                   cATCGGCTTGCTGTTGTACTCCGTTCAGCGGCGGCAGCCGCTTTCGGCCCGGCAGCGGATATTGA‑‑TGTACCGCTTCATCGAATTCATTGGGCGCTGGTCCATGCTGGACATCTTCGTCATTGCCATCCTGGTGGCAGTGGTGAATTTTg                                <  7:187880/149‑1 (MQ=255)
                                                                                                                     tgggCTTGCTGTTTTACTCGGTTCAGCGGCGGCAGGCGCTTTCGGCCCGGCAGCGGATATTGA‑‑TGTACCGCTTCATCGAATTCATTTGGCGCTTGTCCATGCTGGACATCTTCTTCATTGCCATCCTGGTGGCAGTGGTGAATTTTGGc                              <  5:159544/147‑1 (MQ=255)
                                                                                                                     tCGGCTTGCTGTTGGACTCCGTTCAGCGGCGGCAGCCGCTTTCGGCCCGGCAGCGGATATTGA‑‑TGTACCGCTTCATCGAATTCATTGGGCGCTGGTCCATGCTGGACATCTTCGTCATTGCCATCCTGGTGGCAGTGGTGAATTTTGGc                              <  8:330344/149‑1 (MQ=255)
                                                                                                                              tgttgtACTCCGTTCAGCGGCGGCAGCCGCTTTCGGCCCGGCAGCGGAAATTGA‑‑TGTACCGCTTCATCGAATTCATTGGGCGCTGGTCCAAGCTGGACATCTTCCTCATTACCATCCAGGAGGCACTGGTGAATTTTGGCCGAATatc                      >  4:15870/1‑146 (MQ=255)
                                                                                                                               gttgtACTCCGTTCAGCGGCGGCAGCCGCTTTCGGCCCGGCAGCGGATATTGA‑‑TGTACCGCTTCATCGAATTCATTGGGCGCTGGTCCATGCTGGACATCTTCGTCATTGCCATCCtggtgg                                               >  2:271530/1‑122 (MQ=255)
                                                                                                                               gttgtACTCCGTTCAGCGGCGGCAGCCGCTTTCGGCCCGGCAGCGGATATTGA‑‑TGTACCGCTTCATCGAATTCATTGGGCGCTGGTCCATGCTGGACATCTTCGTCATTGCCATCCtggtgg                                               <  1:271530/122‑1 (MQ=255)
                                                                                                                                ttgtACTCCGTTCAGCGGCGGCAGCCGCTTTCGGCCCGGCAGCGGATATTGA‑‑TGTACCGCTTCATCGAATTCATTGGGCGCTGGTCCATGCTGGACATCTTCGTCATTGCCATCCTGGTGGCAGTGGTGAATTTTGGCCGAATAGCCa                    <  4:169771/148‑1 (MQ=255)
                                                                                                                                  gtACTCCGTTCAGCGGCGGCAGCCGCTTTCGGCCCGGCAGCGGATATTGA‑‑TGTACCGCTTCATCGAATTCATTGGGCGCTGGTCCATGCTGGACATCTTCGTCATTGCCATCCTGGTGGCAGTGGTGAATTTTGGCCGAATAGCCAgt                  <  1:153641/148‑1 (MQ=255)
                                                                                                                                      tCCGTTCAGCGGCGGCAGCCGCTTTCGGCCCGGCAGCGGATATTGA‑‑TGTACCGCTTCATCGAATTCATTGGGCGCTGGTCCATGCTGGACATCTTCGTCATTGCCATCCTGGTGGCAg                                            <  4:72707/118‑1 (MQ=255)
                                                                                                                                      tCCGTTCAGCGGCGGCAGCCGCTTTCGGCCCGGCAGCGGATATTGA‑‑TGTACCGCTTCATCGAATTCATTGGGCGCTGGTCCATGCTGGACATCTTCGTCATTGCCATCCTGGTGGCAg                                            >  3:72707/1‑118 (MQ=255)
                                                                                                                                      tCCGTTCAGCGGCGGCAGCCGCTTTCGGCCCGGCAGCGGATATTGA‑‑TGTACCGCTTCATCGAATTCATTGGGCGCTGGTCCATGCTGGACATCTTCGACATTGCCAACCTGGTGGCAGTGGTGAATTTTGGCCGAATAGCCAGTGTCg              >  2:129038/1‑148 (MQ=255)
                                                                                                                                      ggccgaaaGCGGCTGCCGCCGCTTTCGGCCCGGCAGCGGATATTGA‑‑TGTACCGCTTCATCGAATTCATTGGGCGCTGGTCCATGCTGGACATCTTCGTCATTGCCATCCT‑GTGGCAGTGGTGAATTTTGGCCGAATAGCCAGTGTCg              >  6:318504/8‑147 (MQ=255)
                                                                                                                                        cctgcAGCGGCGGCAGCCGCTTTCGGCCCGGCAGCGGATAATGA‑‑TGTACCGCTTCATCGAATTCATTTGGCGCTGGTCCATGCTGGACATCTTCGTCATTGCCATCCTGGTGGCAGTGGTGAATTTTGGCCGAATAGCCAGTGTCGaa            <  8:113446/144‑1 (MQ=255)
                                                                                                                                        cGTTCAGCGGCGGCAGCCGCTTTCGGCCCGGCAGCGGATATTGA‑‑TGTACCGCTTCATCGAATTCATTGGGCGCTGGTCCATGCTGGACATCTTCGTCATTGCCATCCTGGTGGCAGTGGTGAATTTTGGCCGAATAGCCAGTGTCGac            <  6:309501/148‑2 (MQ=255)
                                                                                                                                             aGCGGCGGCAGCCGCTTTCGTCCCTGTAGCGGATATTGA‑‑TGTACCGCTTCATCGAATTCATTGGGCGCTGGTCCATGCTGGACATCTTCGTCATTGCCATCCTGGTGGCAGTGGTGAATTTTGGCCGAATAGCCAGTGTCGAAGCCAAc      <  3:15870/149‑1 (MQ=255)
                                                                                                                                             aGCGGCGGCAGCCGCTTTCGGCCCGGCAGCGGATATTGA‑‑TGTACCGCTTCATCGAATTCATTGGGCGCTGGTCCATGCTGGACATCTTCGTCATTGCCATCCTGGTGGCAGTGGTGAATTTTGGCCGAATAGCCAGTGTCGAAGCCAAc      >  6:48960/1‑149 (MQ=255)
                                                                                                                                                 gcggcAGCCGCTTTCGGCCCGGCAGCGGATATTGA‑‑TGTACCGCTTCATCGAATTCATTGGGCGCTGGTCCATGCTGGACATCTTCGTCATTGCCATCCTGGTGGCAGTGGTGAATTTTGGCCGAATAGCCAGTGTCGAAGCCAACCTgg  <  8:334881/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GTAGCCCGGACACCATCATGTCCGGTGTCATCACCCTGCTCAAGCACGGCATGGTGCCCATTGCGGCTGTGGTGTTCATTGCCAGTATCCTGGTCCCCACGTTCAAGCTGGTGGGCATCGGCTTGCTGTTGTACTCCGTTCAGCGGCGGCAGCCGCTTTCGGCCCGGCAGCGGATATTGA‑‑TGTACCGCTTCATCGAATTCATTGGGCGCTGGTCCATGCTGGACATCTTCGTCATTGCCATCCTGGTGGCAGTGGTGAATTTTGGCCGAATAGCCAGTGTCGAAGCCAACCTGG  >  NC_002947/704131‑704424

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑