Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 711,499 C→G 6.2% L433V (CTG→GTG)  ileS → isoleucine‑‑tRNA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947711,4990CG 6.2% 314.0 / 8.0 97L433V (CTG→GTG) ileSisoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base C (43/48);  new base G (3/3);  total (46/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.44e-01

GAAGCTGAGCGAAGTCGGTGCGCTGATGCACACCGAAACCATCAGC‑CACAGCTACATGCACTGCTGGCGCCACAAGACCCCGCTGATCTACCGCGCCACCGCGCAGTGGTTCGTGGGCATGGACAAGCAGCCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGACACCAAGTTCGTCCCGGCCTGGGGCCAGGCGCGCCTGCACGCGATGATCGCCAACCGCCCGGACTGGTGCATCTCGCGTCAGCGCAACTGGGGTGTGCCGATCCCGTTCTTCCTGCA  >  NC_002947/711351‑711647
                                                                                                                                                       |                                                                                                                                                         
gaAGCTGAGCGAAGTCGGTGCGCTGATGCACACCGAAACCATCAGC‑CACAGCTACATGCACTGCTGGCGCCACAAGACCCCGCTGATCTACCGCGCCACCGCGCAGTGGTTCGGGGGCATGGACAAGCAGCCGAGCACCGGCGAGCCG‑‑c                                                                                                                                                           >  2:142864/1‑149 (MQ=255)
 aaGCTGAGCGAAGTCGGTGCGCTGATGCACACCGAAACCATCAGC‑CACAGCTACTTGCACTGCTGGCGCCACAAGACCCCGCTGATCTACCGCGCCACCGCGCAGTGGTTCGTGGGCATGGACAAGCAGCCGAGCACCGGCGAGCCG‑‑C‑‑‑t                                                                                                                                                       <  3:196975/149‑1 (MQ=255)
      gAGCGAAGTCGGTGCGCTGATGCACACCGAAACCATCAGC‑CACAGCTACATGCACTGCTGGCGCCACAAGACCCCGCTGATCTACCGCGCCACCGCGCAGTGGTTCGTGGGCATGGACAAGCAGCCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGt                                                                                                                                                   >  6:86615/1‑148 (MQ=255)
       aGCGAAGTCGGTGCGCTGATGCACACCGAAACCATCAGC‑CACAGCTACATGCACTGCTGGCGCCACAAGACCCCGCTGATCTACCGCGCCACCGCGCAGTGGTTCGTGGGCATGGACAAGCAGCCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGa                                                                                                                                                 <  8:74408/149‑1 (MQ=255)
        gCGAAGTCGGTGCGCTGATGCACACCGAAACCATCAGC‑CACAGCTACATGCCCTGCTGGCGCCACAAGACCCCGCTGATCTACCGCGCCACCGCGCAGTGGTTCGTGGGCATGGACAAGCAGCCGAGCAGCGGCTCGCCG‑‑G‑‑‑TGctcggc                                                                                                                                                <  7:341127/149‑6 (MQ=255)
         cGAAGTCGGTGCGCTGATGCACACCGAAACCATCAGC‑CACAGCTACATGCACTGCTGGCGCCACAAGACCCCGCTGATCTACCGCGCCACCGCGCAGTGGTTCGTGGGCATGGACAAGCAGCCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGc                                                                                                                                               >  6:12564/1‑149 (MQ=255)
           aaGTCGGTGCGCTGATGCACACCGAAACCATCAGC‑CACAGCTACATGCACTGCTGGCGCCACAAGACCCCGCTGATCTACCGCGCCACCGCGCAGTGGTTCGTGGGCATGGACAAGCAGCCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGt                                                                                                                                             <  2:201602/149‑1 (MQ=255)
                ggTGCGCTGATGCACACCGAAACCATCAGC‑CACAGCTACATGCACTGCTGGCGCCACAAGACCCCGCTGATCTACCGCGCCACCGCGCAGTGGTTCGTGGGCATGGACAAGCAGCCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCt                                                                                                                                        >  5:39808/1‑149 (MQ=255)
                    cgcTGATGCACACCGAAACCATCAGC‑CACAGCTACATGCACTGCTGGCGCCACAAGACCCCGCTGATCTACCGCGCCACCGCGCAGTGGTTCGTGGGCATGGACAAGCAGCCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGaaa                                                                                                                                    <  7:109421/149‑1 (MQ=255)
                    cgcTGATGCACACCGAAACCATCAGC‑CACAGCTACATGCACTGCTGGCGCCACAAGACCCCGCTGATCTACCGCGCCACCGCGCAGTGGTTCGTGGGCATGGACAAGCAGCCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGaaa                                                                                                                                    <  2:273831/149‑1 (MQ=255)
                             acacCGAAACCATCAGC‑CACAGCTACATGCACTGCTGGCGCCACAAGACCCCGCTGATCTACCGCGCCACCGCGCAGTGGTTCGTGGGCATGGACAAGCAGCCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCg                                                                                                                             >  3:175623/1‑147 (MQ=255)
                             acacCGAAACCATCAGC‑CACAGCTACATGCACTGCTGGCGCCACAAGACCCCGCTGATCTACCGCGCCACCGCGCAGTGGTTCGTGGGCATGGACAAGCAGCCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCg                                                                                                                             >  4:199479/1‑147 (MQ=255)
                             acacCGAAACCATCAGC‑CACAGCTACATGCACTGCTGGCGCCACAAGACCCCGCTGATCTACCGCGCCACCGCGCAGTGGTTCGTGGGCATGGACAAGCAGCCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCg                                                                                                                             >  8:145839/1‑147 (MQ=255)
                             acacCGAAACCATCAGC‑CACAGCTACATGCACTGCTGGCGCCACAAGACCCCGCTGATCTACCGCGCCACCGCGCAGTGGTTCGTGGGCATGGACAAGCAGCCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑aa                                                                                                                         <  3:15385/149‑1 (MQ=255)
                             acacCGAAACCATCAGC‑CACAGCTACATGCACTGCTGGAGCCACAAGACCCCGCTGATCTACCGCGCCACCGCGCAGTGGTTCGTGGGCATGGACAAGCAGCCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCg                                                                                                                             >  3:169652/1‑147 (MQ=255)
                               acCGAAACCATCAGC‑CACAGCTACATGCACTGCTGGCGCCACAAGACCCCGCTGATCTACCGCGCCACCGCGCAGTGGTTCGTGGGCATGGACAAGCAGCCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGa                                                                                                                       >  7:89246/1‑149 (MQ=255)
                                 cGAAACCATCAGC‑CACAGCTACATGCACTGCTGGCGCCACAAGACCCCGCTGATCTACCGCGCCACCGCGCAGTGGTTGGTGGGCATGGACAAGCAGCCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGaca                                                                                                                     <  5:309230/149‑1 (MQ=255)
                                 cGAAACCATCAGC‑CACAGCTACATGCACTGCTGGCGCCACAAGACCCCGCTGATCTACCGCGCCACCGCGCAGTGGTTCGTGGGCATGGACAAGCAGCCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGaca                                                                                                                     >  8:340850/1‑149 (MQ=255)
                                 cGAAACCATCAGC‑CACAGCTACATGCACTGCTGGCGCCACAAGACCCCGCTGATCTACCGCGCCACCGCGCAGTGGTTCGTGGGCATGGACAAGCAGCCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGaca                                                                                                                     <  5:12564/149‑1 (MQ=255)
                                 cGAAACCATCAGC‑CACAGCTACATGCACTGCTGGCGCCACAAGACCCCGCTGATCTACCGCGCCACCGCGCAGTGGTTCGTGGGCATGGACAAGCAGCCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGaca                                                                                                                     >  7:95046/1‑149 (MQ=255)
                                  gAAACCATCAGCAGACCGCTACATGCACTGCTGGCGCCACAAGACCCCGCTGATCTACCGCGCCACCGCGCAGTGGTTCGTGGGCATGGACAAGCAGCCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGaca                                                                                                                     <  1:131527/149‑1 (MQ=255)
                                        aTCAGC‑CACAGCTACATGCACTGCTGGCGCCACAAGACCCCGCTGATCTACCGCGCCACCGCGCATTGGTTCGTGGGCATGGACAAGCAGCCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGGGCGTGCGCTGAAAGCCATCG‑‑AAGACACCAAGt                                                                                                               >  5:200659/1‑148 (MQ=255)
                                            gC‑CACAGCTACATGCACTGCTGGCGCCACAAGACCCCGCTGATCTACCGCGCCACCGCGCAGTGGTTCGTGGGCATGGACAAGCAGCCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGACACCAAGTTCGTc                                                                                                          <  1:167792/149‑1 (MQ=255)
                                                acaGCTACATGCACTGCTGGCGCCACAAGACCCCGCTGATCTACCGCGCCACCGCGCAGTGGTTCGTGGGCATGGACAAGCAGCCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGACACCAAGTTCGTCCCg                                                                                                       <  4:175838/149‑1 (MQ=255)
                                                acaGATACATGCACTGCTGGCGCCACAAGACCCCGCTGATCTACCGCGCCACCGCGCAGTGGTTCGTGGGCATGGACAAGCAGCCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGACACCAAGTTCGTCCCg                                                                                                       >  4:293817/1‑149 (MQ=255)
                                                 caGCTACATGCACTGCTGGCGCCACAAGACCCCGCTGATCTACCGCGCCACCGCGCAGTGGTTCGTGGGCATGGACAAGCAGCCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCTCTGAAAGCCATCG‑‑AAGACACCAAGTTCGTCCCgg                                                                                                      <  7:145839/149‑1 (MQ=255)
                                                 caGCTACATGCACTGCTGGCGCCACAAGACCCCGCTGATCTACCGCGCCACCGCGCAGTGGTTCGTGGGCATGGACAAGCAGCCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGaaa                                                                                                                                    >  5:211369/1‑121 (MQ=255)
                                                 caGCTACATGCACTGCTGGCGCCACAAGACCCCGCTGATCTACCGCGCCACCGCGCAGTGGTTCGTGGGCATGGACAAGCAGCCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGaaa                                                                                                                                    <  6:211369/121‑1 (MQ=255)
                                                    cTACATGCACTGCTGGCGCCACAAGACCCCGCTGATCTACCGCGCCACCGCGCAGTGGTTCGTGGGCATGGACAAGCAGCCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGACACCAAGTTCGTCCCGGCCt                                                                                                   >  4:203908/1‑149 (MQ=255)
                                                     tACATGCACTGCTGGCGCCACAAGACCCCGCTGATCTACCGCGCCACCGCGCAGTGGTTCGTGGGCATGGACAAGCAGCCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGACACCAAGTTCTTCCCGGCCTg                                                                                                  >  8:238925/1‑149 (MQ=255)
                                                       cATGCACTGCTGGCGCCACAAGACCCCGCTGATCTACCGCGCCACCGCGCAGTGGTTCGTGGGCATGGACAAGCAGCCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGACACCAAGTTCGTCCCGGCCTggg                                                                                                >  2:36199/1‑149 (MQ=255)
                                                       cATGCACTGCTGGCGCCACAAGACCCCGCTGATCTACCGCGCCACCGCGCAGTGGTTCGTGGGCATGGACAAGCAGCCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGACACCAAGTTCGTCCCGGCCTgg                                                                                                 <  6:96112/148‑1 (MQ=255)
                                                       cATGCACTGCTGGCGCCACAAGACCCCGCTGATCTACCGCGCCACCGCGCAGTGGTTCGTGGGCATGGACAAGCAGCCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGACACCAAGTTCGTCCCGGCCTg                                                                                                  <  5:433/147‑1 (MQ=255)
                                                              tgctgGCGCCACAAGACCCCGCTGATCTACCGCGCCACCGCGCAGTGGTTCGTGGGCATGGACAAGCAGCCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGACACCAAGTTCGTCCCGGCCTGGGGCCAGgc                                                                                         >  3:307633/1‑149 (MQ=255)
                                                                ctgGCGCCACAAGACCCCGCTGATCTACCGCGCCACCGCGCAGTGGTTCGTGGGCATGGACAAGCAGCCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGACACCAAGTTCGTCCCGGCCTGGGGCCAGgc                                                                                         <  4:223841/147‑1 (MQ=255)
                                                                    cgcCACAAGACCCCGCTGATCTACCGCGCCACCGCGCATTGGTTCGTGGGCATGGACAAGCAGCCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGGGCGTGCGCTGAAAGCCATCG‑‑AAGACACCAAGTTCGTCCCGGCCTGGGGCCAGGCGCGCCt                                                                                   <  6:200659/149‑1 (MQ=255)
                                                                     gcCACAAGACCCCGCTGATCTACCGCGCCACCGCGCAGTGGTTCGTGGGCATGGACAAGCAGCCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGTTGAAAGCCATCG‑‑AAGACACCAAGTTCGTCCCGGCCTGGGGCCAGGCGCGCCTg                                                                                  >  4:170200/1‑149 (MQ=255)
                                                                       cacaAGACCCCGCTGATCTACCGCGCCACCGCGCAGTGGTTCGTGGGCATGGACAAGCAGCCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGTTGAAAGCCATCG‑‑AAGACACCAAGTTCGTCCCGGCCTGGGGCCAGGCGCGCCTGCa                                                                                <  3:170200/149‑1 (MQ=255)
                                                                           aGACCCCGCTGATCTACCGCGCCACCGCGCAGTGGTTCGTGGGCATGGACAAGCAGCCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGACACCAAGTTCGTCCCGGCCTggg                                                                                                >  2:183512/1‑129 (MQ=255)
                                                                           aGACCCCGCTGATCTACCGCGCCACCGCGCAGTGGTTCGTGGGCATGGACAAGCAGCCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGACACCAAGTTCGTCCCGGCCTggg                                                                                                <  1:183512/129‑1 (MQ=255)
                                                                            gACCCCGCTGATCTACCGCGCCACCGCGCAGTGGTTCGTGGGCATGGACAAGCAGCCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGACACCAAGTTCGTCCCGGCCTGGGGCCAGGCGCGCCTGCACGCga                                                                           <  1:36199/149‑1 (MQ=255)
                                                                               cccGCTGATCTACCGCGCCACCGCGCAGTGGTTCGTGGGCATGGACAAGCAGCCGAGCACCGGCGAGCCGGCCAGGTGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGACACCAAGTTCGTCCCGGCCTGGGGCCAGGCGCGCCTGCAcgc                                                                             >  1:118731/1‑149 (MQ=255)
                                                                               cccGCTGATCTACCGCGCCACCGCGCAGTGGTTCGTGGGCATGGACAAGCAGCCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGACACCAAGTTCGTCCCGGCCTGGGGCCAGGCGCGCCTGCACGCgatga                                                                        >  6:226719/1‑149 (MQ=255)
                                                                               cccGCTGATCTACCGCGCCACCGCGCAGTGGTTCGTGGGCATGGACAAGCAGCCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGACACCAAGTTCGTCCCGGCCTGGGGCCAGGCGCGCCTGCACGCgatga                                                                        >  6:10002/1‑149 (MQ=255)
                                                                                    tGATCTACCGCGCCACCGCGCAGTGGTTCGTGGGCATGGACAAGCAGCCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGACACCAAGTTCGTcc                                                                                                         <  6:292167/111‑1 (MQ=255)
                                                                                    tGATCTACCGCGCCACCGCGCAGTGGTTCGTGGGCATGGACAAGCAGCCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGACACCAAGTTCGTcc                                                                                                         >  5:292167/1‑111 (MQ=255)
                                                                                    tGATCTACCGCGCCACCGCGCAGTGGTTCGTGGGCATGGACAAGCAGCCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGACACCAAGTTCGTCCCGGCCTGGGGCCAGGCGCGCCTGCACGCGATGATCGc                                                                    >  6:130526/1‑148 (MQ=255)
                                                                                        cTACCGCGCCACCGCGCAGTGGTTCGTGGGCATGGACAAGCAGCCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGACACCAAGTTc                                                                                                             >  6:188593/1‑103 (MQ=255)
                                                                                        cTACCGCGCCACCGCGCAGTGGTTCGTGGGCATGGACAAGCAGCCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGACACCAAGTTc                                                                                                             <  5:188593/103‑1 (MQ=255)
                                                                                           ccGCGCCACCGCGCAGTGGTTCGTGGGCATGGACAAGCAGCCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGACACCAAGTTCGTCCCGGCCTGGGGCCAGGCGCGCCTGCACGCGATGATCGCCaa                                                                 <  7:290033/144‑1 (MQ=255)
                                                                                            ctcgcCACCGCGCAGTGGTTCGTGGGCATGGACAAGCAGCCGATCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGACACCAAGTTCGTCCCGGCCTGGGGCCAGGCGCGCCTGCACGCGATGATCGCCAACCGccc                                                           <  5:204963/147‑1 (MQ=255)
                                                                                                    cgcgCAGTGGTTCGTGGGCATGGACAAGCAGCCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGACACCAAGTTCGTCCCGGCCTGGGGCCAGGCGCGCCTGCACGCGATGATCGCCAACCGCCCGGACTGGt                                                   <  4:292724/149‑1 (MQ=255)
                                                                                                    cgcgCAGTGGTTCGTGGGCATGGACAAGCAGCCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGACACCAAGTTCGTCCCGGCCTGGGGCCAGGCGCGCCTGCACGCGATGATCGCCAACCGCCCGGACTGGt                                                   <  5:10002/149‑1 (MQ=255)
                                                                                                      cgcAGTGGTTCGTGGGCATGGACAAGCAGCCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGACACCAAGTTCGTCCCGGCCTGGGGCCAGGCGCGCCTGCACGCGATGATCGCCAACCGCCCGGACTGGTGc                                                 <  5:130526/149‑1 (MQ=255)
                                                                                                         aGTGGTTCGTGGGCATGGACAAGCAGCCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGACACCAAGTTCGTCCCGGCCTGGGGCCAGGCGCGCCTGCACGCGATGATCGCCAACCGCCCGGACTGGTGCAtc                                              >  3:72053/1‑149 (MQ=255)
                                                                                                         aGTGGTTCGTGGGCATGGACAAGCAGCCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGACACCAAGTTCGTCCCGGCCTGGGGCCAGGCGCGCCTGCACGCGATGATCGCCAACCGCCCGGACTGGTGCAtc                                              <  5:86615/149‑1 (MQ=255)
                                                                                                          gTGGTTCGTGGGCATGGACAAGCAGCCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGACACCAAGTTCGTCCCGGCCTGGGGCCAGGCGCGCCTGCACGCGATGATCGCCAACCGCCCGGACTGGTGCAtct                                             >  7:110881/1‑149 (MQ=255)
                                                                                                             gTTCGTGGGCATGGACAAGCAGCCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGACACCAAGTTCTTCCCGGCCTGGGGCCAGGTGCGCCTGCACGCGATGATCGCCAACCGCCCGGACTGGTGCATCTcgc                                          <  7:238925/149‑1 (MQ=255)
                                                                                                             gTTCGTGGGCATGGACAAGCAGCCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGACACCAAGTTCGTCCCGGCCTGGGGCCAGGCGCGCCTGCACGCGATGATCGCCAACCGCCCGGACTGGTGCATCTcgc                                          <  8:110881/149‑1 (MQ=255)
                                                                                                                 gTGGGCATGGACAAGCAGCCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGACACCAAGTTCGTCCCGGCCTGGGTCCAGGCGCGCCTGCACGCGATGATCGCCACCCGCCCGGACTGGTGCATCTCGCGTCa                                      >  3:252907/1‑149 (MQ=255)
                                                                                                                  tGGGCATGGACAAGCAGCCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGACACCAAGTTCGTCCCGGCCTGGGGCCAGGCGCGCCTGCACGCGATGATCGCCAACCGCCCGGACTGGTGCATCTCGCGTCAg                                     >  2:302627/1‑149 (MQ=255)
                                                                                                                   gggCATGGACAAGCAGCCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGACACCAAGTTCGTCCCGGCCTGGGGCCAGGCGCGCCTGCACGCGATGATCGCCAACCGccc                                                           <  3:210615/126‑1 (MQ=255)
                                                                                                                   gggCATGGACAAGCAGCCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGACACCAAGTTCGTCCCGGCCTGGGGCCAGGCGCGCCTGCACGCGATGATCGCCAACCGccc                                                           >  4:210615/1‑126 (MQ=255)
                                                                                                                    ggCATGGACAAGCAGCCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCGATAAGACACCAAGTTCGTCCCGGCCTGGGGCCAGGCGCGCCTGCACGCGATGATCGCCAACCGCCCGGACTGGTGCATCTCGCGTCAg                                     >  1:108482/1‑149 (MQ=255)
                                                                                                                    ggCATGGACAAGCAGCCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGACACCAAGTTCGTCCCGGCCTGGGGCCAGGCGCGCCTGCACGCGATGATCGCCAACCGCCCGGACTGGTGCATCTCGCGTCAgcg                                   <  5:226719/149‑1 (MQ=255)
                                                                                                                     gCATGGACAAGCAGCCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGACACCAAGTTCGTCCCGGCCTGGGGCCAGGCGCGCCTGCACGCGATGATCGCCAACCGCCCGGACTGGTGCATCTCGCGTCAgcg                                   >  8:46423/1‑148 (MQ=255)
                                                                                                                      cATGGACAAGCAGCCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGACACCAAGTTCGTc                                                                                                          <  8:203235/76‑1 (MQ=255)
                                                                                                                       aTGGACAAGCAGCCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGACACCAAGTTCGTCCCGGCCTGGGGCCAGGCGCGCCTGCACGCGATGATCGCCAACCGCCCGGACTGGTGCATCTCGCGTCAGCGCaa                                <  6:39808/149‑1 (MQ=255)
                                                                                                                       aTGGACAAGCAGCCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGACACCAAGTTCGTCCCGGCCTGGGGCCAGGCGCGCCTGCACGCGATGATCGCCAACCGCCCGGACTGGTGCATCTCGCGTCAGCGCaa                                >  6:161127/1‑149 (MQ=255)
                                                                                                                        tGGACAAGCAGCCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGACACCAAGTTCGTCCCGGCCTGGGGCCAGGCGCGCCTGCACGCGATGATCGCCAACCGCCCGGACTGGTGCATCTCGCGTCAGCGCAAc                               <  3:293817/149‑1 (MQ=255)
                                                                                                                          gACAAGCAGCCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGACACCAAGTTCGTCCCGGCCTGGGGCCAGGCGCGCCTGCACGCGATGATCGCCAACCGCCCGGACTGGTGCATCTCGCGTCAGCGCAACTg                             >  6:197428/1‑149 (MQ=255)
                                                                                                                          gACAAGCAGCCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGACACCAAGTTCGTCCCGGCCTGGGGCCAGGCGCGCCTGCACGCGATGATCGCCAACCGCCCGGACTGGTGCATCTCGCGTCAGCGCAACTg                             >  3:78986/1‑149 (MQ=255)
                                                                                                                           aCAAGCAGCCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGACACCAAGTTCGTCCCGGCCTGGGGCCAGGCGCGCCTGCACGCGATGATCGCCAACCGCCCGGACTGGTGCATCTCGCGTCAGCGCAACTgg                            <  2:3448/149‑1 (MQ=255)
                                                                                                                              agcagcCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGACACCAAGTTCGTCCCGGCCTGGGGCCAGGCGCGCCTGCACGCGATGATCGCCAACCGCCCGGACTGGTGCATCTCGCGTCAGCGCAACTGGGgt                         <  3:199479/149‑1 (MQ=255)
                                                                                                                              agcagcCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGACACCAAGTTCGTCCCGGCCTGGGGCCAGGCGCGCCTGCACGCGATGATCGCCAACCGCCCGGACTGGTGCATCTCGCGTCAGCGCAACTGGGgt                         <  5:197428/149‑1 (MQ=255)
                                                                                                                              agcagcCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGACACCAAGTTCGTCCCGGCCTGGGGCCAGGCGCGCCTGCACGCGATGATCGCCAACCGCCCGGACTGGTGCATCTCGCGTCAGCGCAACTGGGgt                         <  1:142864/149‑1 (MQ=255)
                                                                                                                              agcagcCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGACACCAAGTTCGTCCCGGCCTGGGGCCAGGCGCGCCTGCACGCGATGATCGCCAACCGCCCGGACTGGTGCATCTCGCGTCAGCGCAACTGGGgt                         <  7:340850/149‑1 (MQ=255)
                                                                                                                               gcagcCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGACACCAAGTTCGTCCCGGCCTGGGGCCAGGCGCGCCTGCACGCGATGATCGCCAACCGCCCGGACTGGTGCATCTCGCGTCAGCGCAACTGGGgtg                        >  3:72387/1‑149 (MQ=255)
                                                                                                                                 agcCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGACACCAAGTTCGTCCCGGCCTGGGGCCAGGCGCGCCTGCACGCGATGATCGCCAACCGCCCGGACTGGTGCATCTCGCGTCAgcgc                                  >  4:30636/1‑137 (MQ=255)
                                                                                                                                 agcCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGACACCAAGTTCGTCCCGGCCTGGGGCCAGGCGCGCCTGCACGCGATGATCGCCAACCGCCCGGACTGGTGCATCTCGCGTCAgcgc                                  <  3:30636/137‑1 (MQ=255)
                                                                                                                                   tcacgCAGCGGCTCGCCG‑‑G‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGACACCAAGTTCGTCCCGGCCTGGGGCCAGGCGCGCCTGCACGCGATGATCGCCAACCGCCCGGACTGGTGCATCTCGCGTCAGCGCAACTggg                           >  3:237437/5‑142 (MQ=255)
                                                                                                                                   tcacgCAGCGGCTCGCCG‑‑G‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGACACCAAGTTCGTCCCGGCCTGGGGCCAGGCGCGCCTGCACGCGATGATCGCCAACCGCCCGGACTGGTGCATCTCGCGTCAGCGCAACTggg                           <  4:237437/138‑1 (MQ=255)
                                                                                                                                   tcacgCAGCGGCTCGCCG‑‑G‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGACACCAAGTTCGTCCCGGCCTGGGGCCAGGCGCGCCTGCACGCGATGATCGCCAACCGCCCGGACTGGTGCATCTCGCGTCAGCGCAACTGGGGTGTGcc                    >  2:367818/5‑149 (MQ=255)
                                                                                                                                   ccGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGACACCAAGTTCGTCCCGGCCTGGGGCCAGGCGCGCCTGCACGCGATGATCGCCAACCGCCCGGACTGGTGCATCTCGCGTCAGCGCAACTGGGGTGTGcc                    >  8:218018/1‑149 (MQ=255)
                                                                                                                                     gAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGACACCAAGTTCGTCCCGGCCTGGGGCCAGGCGCGCCTGCACGCGATGATCGCCAACCGCCCGGACTGGTGCATCTCGCGTCAGCGCAACTGGGGTGTGCCGa                  >  2:224765/1‑149 (MQ=255)
                                                                                                                                     acgCAGCGGCTCGCCG‑‑G‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGACACCAAGTTCGTCCCGGCCTGGGGCCAGGCGCGCCTGCACGCGATGATCGCCAACCGCCCGGACTGGTGCATCTCGCGTCAGCGCAACTGGGGTGTGCCGa                  >  1:198321/3‑149 (MQ=255)
                                                                                                                                       gCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGACACCAAGTTCGTCCCGGCCTGGGGCCAGGCGCGCCTGCACGCGATGATCGCCAACCGCCCGGACTGGTGCATCTCGCGTCAGCGCAACTGGGGTGTGCCGATc                <  8:95046/149‑1 (MQ=255)
                                                                                                                                       gCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGACACCAAGTTCGTCCCGGCCTGGGGCCAGGCGCGCCTGCACGCGATGATCGCCAACCGCCCGGACTGGTGCATCTCGCGTCAGCGCAACTGGGGTGTGCCGATc                <  4:78986/149‑1 (MQ=255)
                                                                                                                                         aCCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGACACCAAGTTCGTCCCGGCCTGGGGCCAGGCGCGCCTGCACGCGATGATCGCCAACCGCCCGGACTGGTGCATCTCGCGTCAGCGCAACTGGGGTGTGCCGATccc              <  7:218018/149‑1 (MQ=255)
                                                                                                                                         aCCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGACACCAAGTTCGTCCCGGCCTGGGGCCAGGCGCGCCTGCACGCGATGATCGCCAACCGCCCGGACTGGTGCATCTCGCGTCAGCGCAACTGGGGTGTGCCGATccc              <  3:203908/149‑1 (MQ=255)
                                                                                                                                           cGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGACACCAAGTTCGTCCCGGCCTGGGGCCAGGCGCGCCTGCACGCGATGATCGCCAACCGCCCGGACTGGTGCATCTCGCGTCAGCGCAACTGGGgt                         <  7:113903/136‑1 (MQ=255)
                                                                                                                                           cGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGACACCAAGTTCGTCCCGGCCTGGGGCCAGGCGCGCCTGCACGCGATGATCGCCAACCGCCCGGACTGGTGCATCTCGCGTCAGCGCAACTGGGgt                         >  8:113903/1‑136 (MQ=255)
                                                                                                                                           cGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGACACCAAGTTCGTCCCGGCCTGGGGCCAGGCGCGCCTGCACGCGATGATCGCCAACCGCCCGGACTGGTGCATCTCGCGTCAGCGCAACTGGGGTGTGCCGATCCCGt            >  8:263988/1‑149 (MQ=255)
                                                                                                                                           cGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGACACCAAGTTCGTCCCGGCCTGGGGCCAGGCGCGCCTGCACGCGATGATCGCCAACCGCCCGGACTGGTGCATCTCGCGTCAGCGCAACTGGGGTGTGCCGATCCCGt            >  1:295034/1‑149 (MQ=255)
                                                                                                                                             gCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGACACCAAGTTCGTCCCGGCCTGGGGCCAGGCGCGCCTGCACGCGATGATCGCCAACCGCCCGGACTGGTGCATCTCGCGTCAGCGCAACTGGGgt                         >  2:105350/1‑134 (MQ=255)
                                                                                                                                             gCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGACACCAAGTTCGTCCCGGCCTGGGGCCAGGCGCGCCTGCACGCGATGATCGCCAACCGCCCGGACTGGTGCATCTCGCGTCAGCGCAACTGGGgt                         <  1:105350/134‑1 (MQ=255)
                                                                                                                                                 gCCG‑‑G‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGACACCAAGTTCGTCCCGGCCTGGGGCCAGGCGCGCCTGCACGCGATGATCGCCAACCGCCCGGACTGGTGCATCTCGCGTCAGCGCAACTGGGGTGTGCCGATCCCGttcttc       <  2:198321/148‑1 (MQ=255)
                                                                                                                                                   cG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGACACCAAGTTCGTCCCGGCCTGGGGCCAGGCGCGCCTGCACGCGATGATCGCCAACCGCCCGGACTGGTGCATCTCGCGTCAGCGCAACTGGGGTGTGCCGATCCCGTTCTTCCTg    <  4:72053/149‑1 (MQ=255)
                                                                                                                                                       c‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGACACCAAGTTCGTCCCGGCCTGGGGCCAGGCGCGCCTGCACGCGATGATCGCCAACCGCCCGGACTGGTGCATCTCGCGTCAGCGCAACTGGGGTGTGCCGATCCCGTTCTTCCTGca  >  3:351840/1‑149 (MQ=255)
                                                                                                                                                       |                                                                                                                                                         
GAAGCTGAGCGAAGTCGGTGCGCTGATGCACACCGAAACCATCAGC‑CACAGCTACATGCACTGCTGGCGCCACAAGACCCCGCTGATCTACCGCGCCACCGCGCAGTGGTTCGTGGGCATGGACAAGCAGCCGAGCACCGGCGAGCCG‑‑C‑‑‑TGCGTGAGCGTGCGCTGAAAGCCATCG‑‑AAGACACCAAGTTCGTCCCGGCCTGGGGCCAGGCGCGCCTGCACGCGATGATCGCCAACCGCCCGGACTGGTGCATCTCGCGTCAGCGCAACTGGGGTGTGCCGATCCCGTTCTTCCTGCA  >  NC_002947/711351‑711647

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑