Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	733,647	A→T	6.5%	intergenic (+21/‑26)	PP_t09 → / → PP_t10	tRNA‑Lys/tRNA‑Pro

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	733,647	0	A	T	6.5%	173.3 / 3.1	62	intergenic (+21/‑26)	PP_t09/PP_t10	tRNA‑Lys/tRNA‑Pro
Reads supporting (aligned to +/- strand):  ref base A (30/28);  new base T (2/2);  total (32/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.26e-01

CTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAA‑TCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTT‑TTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAAC  >  NC_002947/733499‑733793                                                                                                                                                      |                                                                                                                                                    cTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGTCTTTTAACCAATTGGTCGTAGGTTCGAA‑TCCTACACGACCCACCATGTAGAAAAGGGCATCTCaaa                                                                                                                                                     <  3:91963/149‑1 (MQ=255) cTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAA‑TCCTACACGACCCACCATGTAGAAAAGGGCATCTCaaa                                                                                                                                                     <  7:24294/149‑1 (MQ=255) cTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAA‑TCCTACACGACCCACCATGTAGAAAAGGGCATCTCaaa                                                                                                                                                     <  7:235402/149‑1 (MQ=255)      ggAGCAGGATGTCAGGAGTTCAAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAA‑TCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGAt                                                                                                                                                <  7:256504/149‑1 (MQ=255)         gCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAA‑TCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGcc                                                                                                                                             <  1:283279/149‑1 (MQ=255)         gCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAA‑TCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGcc                                                                                                                                             <  8:114741/149‑1 (MQ=255)          cAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAA‑TCCTACACGACCCACCATGTAGAAAAGGGCATCTCTTTGAGATGccc                                                                                                                                            <  7:7303/149‑1 (MQ=255)             gATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAA‑TCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCttt                                                                                                                                         >  2:33538/1‑149 (MQ=255)                 tCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATTTCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCttt‑ttt                                                                                                                                     <  1:93816/149‑1 (MQ=255)                 tCAGGAGGTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAA‑TCCTACACGACCCACCATGTAGAAAATGGCATCTCAAAGAGATGCCCttt‑tttt                                                                                                                                    <  4:88252/149‑1 (MQ=255)                  cAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAA‑TCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCttt‑ttttt                                                                                                                                   >  3:209066/1‑149 (MQ=255)                   aGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAA‑TCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCttt‑ttttt                                                                                                                                   <  8:32906/148‑1 (MQ=255)                   aGGAGTTCGAATCACCTCACCCCGACCATTTTCCGGGTCGTTATATCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAA‑TCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCttt‑ttttg                                                                                                                                   <  2:31413/148‑2 (MQ=255)                    ggAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAA‑TCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCttt‑ttt                                                                                                                                     <  2:10559/145‑1 (MQ=255)                    ggAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAA‑TCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCttt‑ttt                                                                                                                                     >  1:10559/1‑145 (MQ=255)                    ggAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAA‑TCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTT‑TTTTTg                                                                                                                                  >  6:324894/1‑148 (MQ=255)                        ttCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAA‑TCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTT‑TTTTTGAAaca                                                                                                                             >  8:60402/1‑149 (MQ=255)                           gAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAA‑TCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTT‑TTTTTGAAACACTc                                                                                                                          <  1:232338/149‑1 (MQ=255)                           gAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAA‑TCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTT‑TTTTTGAAACACTc                                                                                                                          >  3:141961/1‑149 (MQ=255)                                          gACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAA‑TCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTT‑TTTTTGAAACACTCGGGGTGTAGCGCa                                                                                                             >  6:238599/1‑147 (MQ=255)                                          gACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAA‑TCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTT‑TTTTTGAAACACTCGGGGTGTAGCGCa                                                                                                             <  5:238599/147‑1 (MQ=255)                                               ttttCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAA‑TCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGATATGCCCTTTATTTTTGAAACACTCGGGGTGTAGCGCAGTCCgg                                                                                                       <  1:50498/149‑1 (MQ=255)                                               ttttCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAA‑TCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTT‑TTTTTg                                                                                                                                  <  7:107958/121‑1 (MQ=255)                                               ttttCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAA‑TCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTT‑TTTTTg                                                                                                                                  >  8:107958/1‑121 (MQ=255)                                                tttCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAA‑TCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTT‑TTTTTGAAACACTCGGGGTGTAGCGCAGTCCgg                                                                                                       >  8:285177/1‑147 (MQ=255)                                                   ccGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAA‑TCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTT‑TTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAgc                                                                                                   <  4:209066/148‑1 (MQ=255)                                                     gggTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAAACAATTGGTCGTAGGTTCGAG‑TACTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTT‑TTTTTGAAGCACTCGGGGTGTAGCGCAGTCCGGTTgcgcg                                                                                                >  7:298247/1‑149 (MQ=21)                                                      ggTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAA‑TCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTT‑TTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAgcgcgc                                                                                               <  4:141961/149‑1 (MQ=255)                                                          gTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAA‑TCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTT‑TTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGc                                                                                           >  1:280490/1‑149 (MQ=255)                                                               cTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAA‑TCCTAAACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTT‑TTTTTGAAACACTAGGGGTGTAGCGCAGTCCGGTAGCGCGCATGCtttt                                                                                       >  7:50391/1‑147 (MQ=255)                                                                      ggTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAA‑TCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTT‑TTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTa                                                                                                     <  2:250212/127‑1 (MQ=255)                                                                        tAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAA‑TCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTT‑TTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGAt                                                                             <  7:60402/149‑1 (MQ=255)                                                                           agCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAA‑TCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTT‑TTTTTGAAACACTCGGTGTGTATCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTc                                                                          <  4:177648/149‑1 (MQ=255)                                                                            gCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAA‑TCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTT‑TTTTTGAAACACTCGGGGTGTAGCGCAGTCCGTTAGCGCGCCTGCTTTGGGAGCAGGATGTCa                                                                         <  1:33538/149‑1 (MQ=255)                                                                                     ttttTACCAATTGGTCGTAGGTTCGAA‑TCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTT‑TTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAg                                                                                <  4:264727/133‑1 (MQ=255)                                                                                     ttttAACCAATTGGTCGTAGGTTCGAA‑TCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTT‑TTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAg                                                                                >  3:264727/1‑133 (MQ=255)                                                                                     ttttAACCAATTGGTCGTAGGTTCGAA‑TCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTT‑TTTTTGAAACACTCGGGGTGTAGCGCAGTCAGGTAGCGCGCCTGCTTTGGGATCAGGATGTCAGGAGTTCg                                                                 >  7:264389/1‑148 (MQ=255)                                                                                     ctacgaCCAATTGGTCGTAGGTTCGAA‑TCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTT‑TTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCg                                                                 >  1:2547/6‑148 (MQ=255)                                                                                          aCCAATTGGTCGTAGGTTCGAA‑TCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTT‑TTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATccc                                                           >  8:169449/1‑149 (MQ=255)                                                                                          aCCAATTGGTCGTAGGTTCGAA‑TCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTT‑TTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATccc                                                           <  2:280490/149‑1 (MQ=255)                                                                                          aCCAATTGGTCGTAGGTTCGAA‑TCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTT‑TTTTTGAAACACTCGGGGTGTAGCGCAGTCCCGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATccc                                                           >  1:245267/1‑149 (MQ=255)                                                                                          aCCAATTGGTCGTAGGTTCGAA‑TCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTT‑TTTTTGAAAAACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATccc                                                           >  1:250512/1‑149 (MQ=255)                                                                                           ccAATTGGTCGTAGGTTCGAA‑TCCTACACGACCCACAATGTAGAAAAGTGCATCTCAAAGAGATGCCCTTT‑TTTTTGAAACACTAGGGGTGTAGCGCAGTCCGGTATCGCGCCTGCTTTGGGAGCATGATGTCAGGAGTTCGAATcccc                                                          <  8:50391/149‑1 (MQ=37)                                                                                              aTTGGTCGTAGGTTCGAA‑TCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTT‑TTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCa                                                       >  7:52661/1‑149 (MQ=255)                                                                                                  gtagTAGGTTCGAA‑TCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATTCCCTTT‑ATTTTGAAACACTCGGGGTGTAGCGGAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGACAGGAGTTCGAATACCATCAcccc                                                   >  6:363671/4‑149 (MQ=25)                                                                                                        ggTTCGAA‑TCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTT‑TTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCAt                                             >  8:324813/1‑149 (MQ=255)                                                                                                           tCGAA‑TCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTT‑TTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCAtttt                                          >  3:134960/1‑149 (MQ=255)                                                                                                              aa‑TCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTT‑TTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTgg                                       <  2:2547/149‑1 (MQ=255)                                                                                                                 tCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTT‑TTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGt                                     >  8:9758/1‑149 (MQ=255)                                                                                                                    tACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGACGCCCTTT‑TTTTTGAAACACTAGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGt                                  >  2:96834/1‑149 (MQ=255)                                                                                                                      cacGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTT‑TTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGt                                  <  7:285177/147‑1 (MQ=255)                                                                                                                            ccaccaTGTAGAAAAGGGCATCTCAAAGAGATGCCCTTT‑TTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATATTTGGGTCGTTAGCTCAg                          >  7:19394/1‑149 (MQ=255)                                                                                                                              accaTGTAGAAAAGGGCATCTCAAAGAGATGCCCTTT‑TTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGtt                        >  5:283060/1‑149 (MQ=255)                                                                                                                                caTGTAGAAAAGGGCATCTCAAAGAGATGCCCTTT‑TTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGATCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGTCCATTTTTGGGTCGTTAGCTCAGTTgg                      <  8:264389/149‑1 (MQ=255)                                                                                                                                    tAGAAAAGGGCATCTCAAAGAGATGCCCTTT‑TTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTaga                  <  6:326630/149‑1 (MQ=255)                                                                                                                                    aaaaaaaGGGCATCTCTTTGAGATGCCCTTT‑TTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCGTCACCCCGACCATTTTTGGGTGGTTAGGTCAGGTGGTa                    >  4:112105/4‑147 (MQ=18)                                                                                                                                     aGAAAAGGGCATCTCAAAGAGATGCCCTTT‑TTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTaga                  <  6:283060/148‑1 (MQ=255)                                                                                                                                       gaaaGGGCATCTCAAAGAGATGCCCTTT‑TTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTagag                 >  7:159640/2‑147 (MQ=255)                                                                                                                                        aaaGGGCATCTCTTTGAGATGCCCTTT‑TTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTggg                                      <  4:188577/125‑1 (MQ=255)                                                                                                                                        aaaGGCCATCTCTTTGAGATGCCCTTT‑TTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTggg                                      >  3:188577/1‑125 (MQ=255)                                                                                                                                         aaggtgaTCTCAAAGAGATGCCCTTT‑TTTTTGAAAGACTCGGGGTGTAGCGCAGTCCTGTAGGGCGCCTGCTTTTGGTGCAGGATGTCAGGAGTTCGAATGCGCTCTGCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGt             <  5:363671/143‑1 (MQ=18)                                                                                                                                           gggCATCTCAAAGAGATGCCCTTT‑TTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGCCCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTg           >  3:18623/1‑149 (MQ=255)                                                                                                                                                tctcAAAGAGATGCCCTTT‑TTTTTGAAACACTCGGGATGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCttt      >  5:117644/1‑149 (MQ=255)                                                                                                                                                   cAAAGAGATGCCCTTT‑TTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCAtttt                                          <  2:240919/110‑1 (MQ=255)                                                                                                                                                   cAAAGAGATGCCCTTT‑TTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCAtttt                                          >  1:240919/1‑110 (MQ=255)                                                                                                                                                    aaaGAGATGCCCTTT‑TTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTa    <  7:169449/147‑1 (MQ=255)                                                                                                                                                    aaaGAGATGCCCTTT‑TTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAAc  <  8:52661/149‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                    CTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAA‑TCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTT‑TTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAAC  >  NC_002947/733499‑733793

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑