| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 739,594 | T→G | 5.2% | T345P (ACG→CCG) | pilC ← | type IV fimbrial assembly protein PilC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 739,594 | 0 | T | G | 5.2% | 305.4 / 5.0 | 96 | T345P (ACG→CCG) | pilC | type IV fimbrial assembly protein PilC |
| Reads supporting (aligned to +/- strand): ref base T (42/49); new base G (3/2); total (45/51) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.63e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.24e-01 | |||||||||||
GCAGGTACATCGCCACCACCAGGCCACCCACCAGCAAGCCCAGGATCAGCACGATGGCCGGCTCCAGCAGGCTGGTCAACTGATCCAGCGCCTGGCTGACCTGTTCCTCGTAATGGCTGGCGGCTTTTTCCAGCATCTGG‑TCCAGC‑GTGCCGCTGGATTCACCGATGGCCGTCAACTGCACCAGCAAGGGGGGGAACAGCGGTTCACCGGCCATCGCCTGGTTCAGCCCTTGCCCATTGGCCATGCCTTGGCGCAGCCGCAACACCGCCTGCTCGTGCAGGTCGCCACCCGTTACC > NC_002947/739447‑739742 | gCAGGTACATCGCCACCACCAGGCCACCCACCAGCAAGCCCAGGATCAGCACGATGGCCGGCTCCAGCAGGCTGGTCAACTGATCCAGCGCCTGGCTGACCTGTTCCTCGTAATGGCTGGCGGCTTTTTCCAGCATCTGG‑TCCAGC‑GTg < 1:163148/149‑1 (MQ=255)gCAGGTACATCGCCACCACCAGGCCACCCACCAGCAAGCCCAGGATCAGCACGATGGCCGGCTCCAGCAGGCTGGTCAACTGATCCAGCGCCTGGCTGACCTGTTCCTCGTAATGGCTGGCGGCTTTTTCCAGCATCTGG‑TCCAGC‑GTg > 4:366689/1‑149 (MQ=255) cAGGTACATCGCCACCACCAGGCCACCCACCAGCAAGCCCAGGATCAGCACGATGGCCGGCTCCAGCAGGCTGGTCAACTGATCCAGCGCCTGGCTGACCTGTTCCTCGTAATGGCTGGCGGCTTTTTCCAGCATCTGG‑TCCAGC‑GTGc < 3:366689/149‑1 (MQ=255) aCATCGCCACCACCAGGCCACCCACCAGCAAGCCCAGGATCAGCACGATGGCCGGCTCCAGCAGGCTGGTCAACTGATCCAGCGCCTGGCTGACCTGTTCCTCGTAATGGCTGGCGGCTTTTTCCAGCATCTGG‑TCCAGC‑GTGCCGCTg > 3:220370/1‑149 (MQ=255) aTCGCCACCACCAGGCCACCCACCAGCAAGCCCAGGATCAGCACGATGGCCGGCTCCAGCAGGCTGGTCAACTGATCCAGCGCCTGGCTGACCTGTTCCTCGTAATGGCTGGCGGCTTTTTCCAGCATCTGG‑TCCAGC‑GTGCCGCTGGa > 5:105863/1‑149 (MQ=255) aTCGCCACCACCAGGCCACCCACCAGCAAGCCCAGGATCAGCACGATGGCCGGCTCCAGCAGGCTGGTCAACTGATCCAGCGCCTGGCTGACCTGTTCCTCGTAATGGCTGGCGGC‑TTTTCCAGCATCTGG‑TCCAGC‑GGCACGCTGGac < 2:368820/149‑2 (MQ=255) tCGCCACCACCAGGCCACCCACCAGCAAGCCCAGGATCAGCACGATGGCCGGCTCCAGCAGGCTGGTCAACTGATCCAGCGCCTGGCTGACCTGTTCCTCGTAATGGCTGGCGGCTTTTTCCAGCATCTGG‑TCCAGC‑GTGCCGCTGGAt < 8:50672/149‑1 (MQ=255) caccaccaGGCCACCCACCAGCAAGCCCAGGATCAGCACGATGGCCGGCTCCAGCAGGCTGGTCAACTGATCCAGCGCCTGGCTGACCTGTTCCTCGTAATGGCTGGCGGCTTTTTCCAGCATCTGG‑TCCAGC‑GTGCCGCTGGATTCAc < 3:148325/149‑1 (MQ=255) caccaccaGGCCACCCACCAGCAAGCCCAGGATCAGCACGATGGCCGGCTCCAGCAGGCTGGTCAACTGATCCAGCGCCTGGCTGACCTGTTCCTCGTAATGGCTGGCGGCTTTTTCCAGCATCTGG‑TCCAGC‑GTGCCGCTGGATTCAc < 1:102484/149‑1 (MQ=255) caccaccaGGCCACCCACCAGCAAGCCCAGGATCAGCACGATGGCCGGCTCCAGCAGGCTGGTCAACTGATCCAGCGCCTGGCTGACCTGTTCCTCGTAATGGCTGGCGGCTTTTTCCAGCATCTGG‑TCCAGC‑GTGCCGCTGGATTCAc > 3:334742/1‑149 (MQ=255) ccaccaGGCCACCCACCAGCAAGCCCAGGATCAGCACGATGGCCGGCTCCAGCAGGCTGGTCAACTGATCCAGCGCCTGGCTGACCTGTTCCTCGTAATGGCTGGCGGCTTTTTCCAGCATCTGG‑TCCAGC‑GGCACGCTGGaccagatg < 5:150917/149‑8 (MQ=255) caccaGGCCACCCACCAGCAAGCCCAGGATCAGCACGATGGCCGGCTCCAGCAGGCTGGTCAACTGATCCAGCGCCTGGCTGACCTGTTCCTCGTAATGGCTGGCGGCTTTTTCCAGCATCTGG‑TCCAGC‑GTGCCGCTGGATTCACCGa > 3:317389/1‑149 (MQ=255) ccaGGCCACCCACCAGCAAGCCCAGGATCAGCACGATGGCCGGCTCCAGCAGGCTGGTCAACTGATCCAGCGCCTGGCTGACCTGTTCCTCGTAATGGCTGGCGGCTTTTTCCAGCATCTGG‑TCCAGC‑GTGCCGCTGGATTCACCGATg < 6:67472/149‑1 (MQ=255) caGGCCACCCACCAGCAAGCCCAGGATCAGCACGATGGCCGGCTCCAGCAGGCTGGTCAACTGATCCAGCGCCTGGCTGACCTGTTCCTCGTAATGGCTGGCGGCTTTTTCCAGCATCTGG‑TCCAGC‑GTGCCGCt > 3:373486/1‑135 (MQ=255) caGGCCACCCACCAGCAAGCCCAGGATCAGCACGATGGCCGGCTCCAGCAGGCTGGTCAACTGATCCAGCGCCTGGCTGACCTGTTCCTCGTAATGGCTGGCGGCTTTTTCCAGCATCTGG‑TCCAGC‑GTGCCGCt < 4:373486/135‑1 (MQ=255) caGGCCACCCACCAGCAAGCCCAGGATCAGCACGATGGCCGGCTCCAGCAGGCTGGTCAACTGATCCAGCGCCTGGCTGACCTGTTCCTCGTAATGGCTGGCGGCTTTTTCCAGCATCTGG‑TCCAGC‑GTGCCGCTGGATTCACCGATgg > 4:31835/1‑149 (MQ=255) aGGCCACCCACCAGCAAGCCCAGGATCAGCACGATGGCCGGCTCCAGCAGGCTGGTCAACTGATCCAGCGCCTGGCTGACCTGTTCCTCGTAATGGCTGGCGGCTTTTTCCAGCATCTGG‑TCCAGC‑GTGCCGCTGGATTCACCGATGGc < 2:351436/149‑1 (MQ=255) ggCCACCCACCAGCAAGCCCAGGATCAGCACGATGGCCGGCTCCAGCAGGCTGGTCAACTGATCCAGCGCCTGGCTGACCTGTTCCTCGTAATGGCTGGCGGCTTTTTCCAGCATCTGG‑TCCAGC‑GTGCCGCTGGATTCACCGATGGcc < 2:15357/149‑1 (MQ=255) ggCCACCCACCACCAATCCCAGGATCAGCACGATGGCCGGCTCCAGCTGGCTGGTCAACTGATCCAGCGCCTGGCTGACCTGTTCCTCGTAATGGCTGGCGGCTTTTTCCAGCATCTGGCTCCAGC‑GTGCCGCTGGATTCACCGATGGc < 1:219755/149‑1 (MQ=255) cacccaccAGCAAGCCCAGGATCAGCACGATGGCCGGCTCCAGCAGGCTGGTCAACTGATCCAGCGCCTGGCTGACCTGTTCCTCGTAATGGCTGGCGGCTTTTTCCAGCATCTGG‑TCCAGC‑GTGCCGCTGGATTCACCGATGGCCGTc < 6:174574/149‑1 (MQ=255) acccaccAGCAAGCCCAGGATCAGCACGATGGCCGGCTCCAGCAGGCTGGTCAACTGATCCAGCGCCTGGCTGACCTGTTCCTCGTAATGGCTGGCGGCTTTTTCCAGCATCTGG‑TCCAGC‑GTGCCGCTGGATTCACCGATGGCAGTc > 2:24302/1‑148 (MQ=255) ccaccaGCAAGCCCAGGATCAGCACGATGGCCGGCTCCAGCAGGCTGGTCAACTGATCCAGCGCCTGGCTGACCTGTTCCTCGTAATGGCTGGCGGCTTTTTCCAGCATCTGG‑TCCAGC‑GTGCCGCTGGATTCACCGATGGCCGTCAAc > 7:84878/1‑149 (MQ=255) ccaccaGCAAGCCCAGGATCAGCACGATGGCCGGCTCCAGCAGGCTGGTCAACTGATCCAGCGCCTGGCTGACCTGTTCCTCGTAATGGCTGGCGGCTTTTTCCAGCATCTGG‑TCCAGC‑GTGCCGCTGGATTCACCGATGGCCGTCAAc > 5:12831/1‑149 (MQ=255) ccaccaGCAAGCCCAGGATCAGCACGATGGCCGGCTCCAGCAGGCTGGTCAACTGATCCAGCGCCTGGCTGACCTGTTCCTCGTAATGGCTGGCGGCTTTTTCCAGCATCTGG‑TCCAGC‑GTGCCGCTGGATTCACCGATGGCCGTCAAc > 3:294498/1‑149 (MQ=255) aGCAAGCCCAGGATCAGCACGATGGCCGGCTCCAGCAGGCTGGTCAACTGATCCAGCGCCTGGCTGACCTGTTCCTCGTAATGGCTGGCGGCTTTTTCCAGCATCTGG‑TCCAGC‑GTGCCGCTGGATTCACCGATGGCCGTCAACTGCAc < 4:317389/149‑1 (MQ=255) ccAGGATCAGCACGATGGCCGGCTCCAGCAGGCTGGTCAACTGATCCAGCGCCTGGCTGACCTGTTCCTCGTAATGGCTGGCGGCTTTTTCCAGCATCTGG‑TCCAGC‑GTGCCGCTGGATTCACCGATGGCCGTCAACTGCACCAGCAAg > 1:379403/1‑149 (MQ=255) cAGGATCAGCACGATGGCCGGCTCCAGCAGGCTGGTCAACTGATCCAGCGCCTGGCTGACCTGTTCCTCGTAATGGCTGGCGGCTTTTTCCAGCATCTGG‑TCCAGC‑GTGCCGCTGGATTCACCGATGGCCGTCAACTGCACCAGCAAgg > 1:46923/1‑149 (MQ=255) aGGATCAGCACGATGGCCGGCTCCAGCAGGCTGGTCAACTGATCCAGCGCCTGGCTGACCTGTTCCTCGTAATGGCTGGCGGCTTTTTCCAGCATCTGG‑TCCAGC‑GTGCCGCTGGATTCACCGATGGCCGTCAACTGCACCAGCAAggg < 3:31835/149‑1 (MQ=255) gATCAGCACGATGGCCGGCTCCAGCAGGCTGGTCAACTGATCCAGCGCCTGGCTGACCTGTTCCTCGTAATGGCTGGCGGCTTTTTCCAGCATCTGG‑TCCAGC‑GTGCCGCTGGATTCACCGATGGCCGTCAACTGCACCAGCAAggggg < 1:191072/149‑1 (MQ=255) aGCACGATGGCCGGCTCCAGCAGGCTGGTCAACTGATCCAGCGCCTGGCTGACCTGTTCCTCGTAATGGCTGGCGGCTTTTTCCAGCATCTGG‑TCCAGC‑GTGCCGCTGGATTCACCGATGGCCGTCAACTGCACCAGCAAGGGGGGGaa > 6:48617/1‑149 (MQ=255) gCACGATGGCCGGCTCCAGCAGGCTGGTCAACTGATCCAGCGCCTGGCTGACCTGTTCCTCGTAATGGCTGGCGGC‑TTTTCCAGCATCTGG‑TCCAGC‑GTGCCGCTGGATTCACCGATGGCCGTCAACTGCACCAGCAAGGGGGGGaa > 3:289491/1‑147 (MQ=255) gATGGCCGGCTCCAGCAGGCTGGTCAACTGATCCAGCGCCTGGCTGACCTGTTCCTCGTAATGGCTGGCGGCTTTTTCCAGCATCTGG‑TCCAGC‑GTGCCGCTGGATTCACCGATGGCCGTCAACTGCACCAGCAAGGGGGGGAACAGCg > 8:328731/1‑149 (MQ=255) gATGGCCGGCTCCAGCAGGCTGGTCAACTGATCCAGCGCCTGGCTGACCTGTTCCTCGTAATGGCTGGCGGCTTTTTCCAGCATCTGG‑TCCAGC‑GTGCCGCTGGATTCACCGATGGCCGTCAACTGCACCAGCAAGGGGGGGAACAGCg > 4:195881/1‑149 (MQ=255) tGGCCGGCTCCAGCAGGCTGGTCAACTGATCCAGCGCCTGGCTGACCTGTTCCTCGTAATGGCTTGCGGCTTTTTCCAGCATCTGG‑TCCAGC‑GTGCCGCt < 6:89363/100‑1 (MQ=255) tGGCCGGCTCCAGCAGGCTGGTCAACTGATCCAGCGCCTGGCTGACCTGTTCCTCGTAATGGCTCGCGGCTTTTTCCAGCATCTGG‑TCCAGC‑GTGCCGCt > 5:89363/1‑100 (MQ=255) tGGTCAACTGATCCAGCGCCTGGCTGACCTGTTCCTCGTAATGGCTGGCGGCTTTTTCCAGCATCTGG‑TCCAGC‑GTGCCGCTGGATTCACCGATGGCCGTCAACTGCACCAGCAAgggg > 4:230524/1‑119 (MQ=255) tGGTCAACTGATCCAGCGCCTGGCTGACCTGTTCCTCGTAATGGCTGGCGGCTTTTTCCAGCATCTGG‑TCCAGC‑GTGCCGCTGGATTCACCGATGGCCGTCAACTGCACCAGCAAgggg < 3:230524/119‑1 (MQ=255) tGGTCAACTGATCCAGCGCCTGGCTGACCTGTTCCTCGTAATGGCTGGCGGCTTTTTCCAGCATCTGG‑TCCAGC‑GTGCCGCTGGATTCACCGATGGCCGTCAACTGCACCAGCAAGGGGGGGAACAGCGGTTCACCGGCCATCGCCTgg > 5:204291/1‑149 (MQ=255) gTCAACTGATCCAGCGCCTGGCTGACCTGTTCCTCGTAATGGCTGGCGGCTTTTTCCAGCATCTGG‑TCCAGC‑GTGCCGCTGGATTCACCGATGGCCGTCAACTGCACCAGCAAGGGGGGGAACAGCGGTTCACCGGCCATCGCCTGGtt > 8:213411/1‑149 (MQ=255) tCAACTGATCCAGCGCCTGGCTGACCTGTTCCTCGTAATGGCTGGCGGCTTTTTCCAGCATCTGG‑TCCAGC‑GTGCCGCTGGATTCACCGATGGCCGTCAACTGCACCAGCAAGGGGGGGAACAGCGGTTCACCGGCCATCGCCTGGtt > 6:16411/1‑148 (MQ=255) tCAACTGATCCAGCGCCTGGCTGACCTGTTCCTCGTAATGGCTGGCGGCTTTTTCCAGCATCTGG‑TCCAGC‑GTGCCGCTGGATTCACCGATGGCCGTCAACTGCACCAGCAAGGGGGGGAACAGCGGTTCACCGGCCATCGCCTGGtt > 1:244012/1‑148 (MQ=255) aaCTGATCCATCGCCTGGCTGACCTGTTCCTCGTAATGGCTGGCGGCTTTTTCCAGCATCTGG‑TCCAGC‑GTGCCGCTGGATTCACCGATGGCCGTCAACTGCACCAGCAAGGGGGGGAACAGCGGTTCACCGGCCATCGCCTGGTTCa < 1:361073/148‑1 (MQ=255) aaCTGATCCAGCGCCTGGCTGACCTGTTCCTCGTAATGGCTGGCGGCTTTTTCCAGCATCTGG‑TCCAGC‑GTGCCGCTGGATTCACCGATGGCCGTCAACTGCACCAGCAAgggg > 1:376736/1‑114 (MQ=255) aaCTGATCCAGCGCCTGGCTGACCTGTTCCTCGTAATGGCTGGCGGCTTTTTCCAGCATCTGG‑TCCAGC‑GTGCCGCTGGATTCACCGATGGCCGTCAACTGCACCAGCAAgggg < 2:376736/114‑1 (MQ=255) aCTGATCCAGCGCCTGGCTGACCTGTTCCTCGTAATGGCTGGCGGCTTTTTCCAGCATCTGG‑TCCAGC‑GTGCCGCTGGATTCACCGATGGCCTTCAACTGCACCAGCAAGGGGGGGAACAGCGGTTCACCGGCCATCGCCTGGTTCAGc < 1:105052/149‑1 (MQ=255) aCTGATCCAGCGCCTGGCTGACCTGTTCCTCGTAATGGCTGGCGGCTTTTTCCAGCATCTGG‑TCCAGC‑GTGCCGCTGGATTCACCGATGGCCGTCAACTGCACCAGCAAGGGGGGGAACAGCGGTTCACCGGCCATCGCCTGGTTCAGc > 2:186302/1‑149 (MQ=255) tGATCCAGCGCCTGGCTGACCTGTTCCTCGTAATGGCTGGCGGCTTTTTCCAGCATCTGG‑TCCAGC‑GTGCCGCTGGATTCACCGATGGCCGTCAACTGCACCAGCAAGGGGGGGAACAGCGGTTCACCGGCCATCGCCTGGTTCAGccc > 1:277534/1‑149 (MQ=255) aTCCAGCGCCTGGCTGACCTGTTCCTCGTAATGGCTGGCGGCTTTTTCCAGCATCTGG‑TCCAGCGGGGCCGCTGGATTCACCGATGGCCGTCAACTGCACCAGCAAGGGGGGGAACAGCGGTTCACCGGCCATCGCCTCGTTCAGCCCt > 1:302086/1‑149 (MQ=255) aTCCAGCGCCTGGCTGACCTGTTCCTCGTAATGGCTGGCGGCTTTTTCCAGCATCTGG‑TCCAGC‑GTGCCGCTGGATTCACCGATGGCCGTCAACTGCACCAGCAAGGGGGGGAACAGCGGTTCACCGGCCATCGCCTGGTTCAGccc < 6:63396/147‑1 (MQ=255) aTCCAGCGCCTGGCTGACCTGTTCCTCGTAATGGCTGGCGGCTTTTTCCAGCATCTGG‑TCCAGC‑GTGCCGCTGGATTCACCGATGGCCGTCAACTGCACCAGCAAGGGGGGGAACAGCGGTTCACCGGCCATCGCCTGGTTCAGCCCtt < 4:294498/149‑1 (MQ=255) aTCCAGCGCCTGGCTGACCTGTTCCTCGTAATGGCTGGCGGCTTTTTCCAGCATCTGG‑TCCAGC‑GTGCCGCTGGATTCACCGATGGCCGTCAACTGCACCAGCAAGGGGGGGAACAGCGGTTCACCGGCCATCGCCTGGTTCAGCCCtt < 2:379403/149‑1 (MQ=255) tCCAGCGCCTGGCTGACCTGTTCCTCGTAATGGCTGGCGGCTTTTTCCAGCATCTGG‑TCCAGC‑GTGCCGCTGGATTCACCGATGGCCGTCAACTGCACCAGCAAGGGGGGGAACAGCGGTTCACCGGCCATCGCCTGGTTCAGCCCTTg < 1:167194/149‑1 (MQ=255) cAGCGCCTGGCTGACCTGTTCCTCGTAATGGCTGGCGGCTTTTTCCAGCATCTGG‑TCCAGC‑GTGCCGCTGGATTCACCGATGGCCGTCAACTGCACCAGCAAGGGGGGGAACAGCGGTTCACCGGCCATCGCCTGGTTCAGCCCTTGcc > 2:143861/1‑149 (MQ=255) cAGCGCCTGGCTGACCTGTTCCTCGTAATGGCTGGCGGCTTTTTCCAGCATCTGG‑TCCAGC‑GTGCCGCTGGATTCACCGATGGCCGTCAACTGCACCAGCAAGGGGGGGAACAGCGGTTCACCGGCCATCGCCTGGTTCAGCCCTTGcc < 7:328731/149‑1 (MQ=255) ccTGGCTGACCTGTTCCTCGTAATGGCTGGCGGCTTTTTCCAGCATCTGG‑TCCAGC‑GTGCCGCTGGATTCACCGATGGCCGTCAACTGCACCAGCAAGGGGGGGAACAGCGGTTCACCGGCCATCGCCTGGTTCAGCCCTTGCCCATTg < 2:170293/149‑1 (MQ=255) cTGGCTGACCTGTTCCTCGTAATGGCTGGCGGCTTTTTCCAGCATCTGG‑TCCAGC‑GTGCCGCTGGATTCACCGATGGCCGTCAACTGCACCAGCAAGGGGGGGAACAGCGGTTCACCGGCCATCGCCTGGTTCAGCCCTTGCCCATTgg > 3:324274/1‑149 (MQ=255) tGGCTGACCTGTTCCTCGTAATGGCTGGCGGCTTTTTCCAGCATCTGG‑TCCAGC‑GTGCCGCTGGATTCACCGATGGCCGTCAACTGCACCAGCAAGGGGGGGAACAGCGGTTCACCGGCCATCGCCTGGTTCAGCCCTTGCCCATTGGc > 3:70657/1‑149 (MQ=255) ggCTGACCTGTTCCTCGTAATGGCTGGCGGCTTTTTCCAGCATCTGG‑TCCAGC‑GTGCCGCTGGATTCACCGATGGCCGTCAACTGCACCAGCAAGGGGGGGAACAGCGGTTCACCGGCCATCGCCTGGTTCAGCCCTTGCCCATTGGc > 3:294283/1‑148 (MQ=255) gCTGACCTGTTCCTCGTAATGGCTGGCGGCTTTTTCCAGCATCTGG‑TCCAGC‑GTGCCGCTGGATTCACCGATGGCCGTCAACTGCACCAGCAAGGGGGGGAACAGCGGTTCACCGGCCATCGCCTGGTTCAGCCCTTGCCCATTGGc < 4:70657/147‑1 (MQ=255) cTGACCTGTTCCTCGTAATGGCTGGCGGCTTTTTCCAGCATCTGG‑TCCAGC‑GTGCCGCTGGATTCACCGATGGCCGTCAACTGCACCAGCAAGGGGGGGAACAGCGGTTCACCGGCCATCGCCTGGTTCAGCCCTTGCCCATTGGCCAt < 5:191368/149‑1 (MQ=255) ttCCTCGTAATGGCTGGCGGCTTTTTCCAGCATCTGG‑TCCAGC‑GTGCCGCTGGATTCACCGATGGCCGTCAACTGCACCAGCAAGGGGGGGAACAGCGGTTCACCGGCCATCGCCTGGTTCAGCCCTTGCCCATTGGCCATGCCTTgg < 3:195881/148‑1 (MQ=255) ttCCTCGTAATGGCTGGCGGCTTTTTCCAGCATCTGG‑TCCAGC‑GTGCCGCTGGATTCACCGATGGCCGTCAACTGCACCAGCAAGGGGGGGAACAGCGGTTCACCGGCCATCGCCTGGTTCAGCCCTTGCCCATTGGCCATGCCTTGgc > 4:340453/1‑149 (MQ=255) ccTCGTAATGGCTGGCGGCTTTTTCCAGCATCTGG‑TCCAGC‑GTGCCGCTGGATTCACCGATGGCCGTCAACTGCACCAGCAAGGGGGGGAACAGCGGTTCACCGGCCATCGCCTGGTTCAGCCCTTGCCCATTGGCCATGCCTTGgcg > 5:137941/1‑148 (MQ=255) cTCGTAATGGCTGGCGGCTTTTTCCAGCATCTGG‑TCCAGC‑GTGCCGCTGGATTCACCGATGGCCGTCAACTGCACCAGCAAGGGGGGGAACAGCGGTTCACCGGCCATCGCCTGGTTCAGCCCTTGCCCATTGGCCATGCCTTGgcg > 1:6400/1‑147 (MQ=255) gTAATGGCTGGCGGCTTTTTCCAGCATCTGG‑TCCAGC‑GTGCCGCTGGATTCACCGATGGCCGTCAACTGCACCAGCAAGGGGGGGAACAGCGGTTCACCGGCCATCGCCTGGTTCa < 4:132042/116‑1 (MQ=255) gTAATGGCTGGCGGCTTTTTCCAGCATCTGG‑TCCAGC‑GTGCCGCTGGATTCACCGATGGCCGTCAACTGCACCAGCAAGGGGGGGAACAGCGGTTCACCGGCCATCGCCTGGTTCAGCCCTTGCCCATTGGCCATGCCTTGGCGCAg < 2:6400/147‑1 (MQ=255) tAATGGCTGGCGGCTTTTTCCAGCATCTGG‑TCCAGC‑GTGCCGCTGGATTCACCGATGGCCGTCAACTGCACCAGCAAGGGGGGGAACAGCGGTTCACCGGCCATCGCCTGGTTCAGCCCTTGCCCATTGGCCATGCCTTGGCGCAGCCg > 2:267360/1‑149 (MQ=255) aTGGCTGGCGGCTTTTTCCAGCATCTGG‑TCCAGC‑GTGCCGCTGGATTCACCGATGGCCGTCAACTGCACCAGCAAGGGGGGGAACAGCGGTTCACCGGCCATCGCCTGGTTCAGCCCTTGCCCATTGGCCATGCCTTGGCGCAGCCGc < 8:84878/148‑1 (MQ=255) aTGGCTGGCGGCTTTTTCCAGCATCTGG‑TCCAGC‑GTGCCGCTGGATTCACCGATGGCCGTCAACTGCACCAGCAAGGGGGGGAACAGCGGTTCACCGGCCATCGCCTGGTTCAGCCCTTGCCCATTGGCCATGCCTTGGCGCAGCCGc < 2:46923/148‑1 (MQ=255) tggctggcGGCTTTTTCCAGCATCTGG‑TCCAGC‑GTGCCGCTGGATTCACCGATGGCCGTCAACTGCACCAGCAAGGGGGGGAACAGCGGTTCACCGGCCATCGCCTGGTTCAGCCCTTGCCCATTGGCCATGCCTTGGCGCAGCCGCaa < 4:334742/149‑1 (MQ=255) gctggcGGCTTTTTCCAGCATCTGG‑TCCAGC‑GTGCCGCTGGATTCACCGATGGCCGTCAACTGCACCAGCAAGGGGGGGAACAGCGGTTCACCGGCCATCGCCTGGTTCAGCCCTTGCCCATTGGCCATGCCTTGGCGCAGCCGCAaca < 5:48617/149‑1 (MQ=255) gcggcTTTTTCCAGCATCTGG‑TCCAGC‑GTGCCGCTGGATTCACCGATGGCCGTCAACTGCACCAGCAAGGGGGGGAACAGCGGTTCACCGGCCATCGCCTGGTTCAGCCCTTGCCCATTGGCCATGCCTTGGCGCAGCCGCAACACCGc > 7:196247/1‑149 (MQ=255) cggcTTTTTCCAGCATCTGG‑TCCAGC‑GTGCCGCTGGATTCACCGATGGCCGTCAACTGCACCAGCAAGGGGGGGAACAGCGGTTCACCGGCCATCGCCTGGTTCAGCCCTTGCCCATTGGCCATGCCTTGGCGCAGCCGCAACACCGcc > 1:240401/1‑149 (MQ=255) cTTTTTCCAGCATCTGG‑TCCAGC‑GTGCCGCTGGATTCACCGATGGCCGTCAACTGCACCAGCAAGGGGGGGAACAGCGGTTCACCGGCCATCGCCTGGTTCAGCCCTTGCCCATTGGCCATGCCTTGGCGCAGCCGCAACACCGCCTGc < 2:244012/149‑1 (MQ=255) tttCCAGCATCTGG‑TCCAGC‑GTGCCGCTGGATTCACCGATGGCCGTCAACTGCACCAGCAAGGGGGGGAACAGCGGTTCACCGGCCATCGCCTGGTTCAGCCCTTGCCCATTGGCCATGCCTTGGCGCAGCCGCAACACCGCCTGCTc < 6:137941/148‑1 (MQ=255) cAGCATCTGG‑TCCAGC‑GTGCCGCTGGATTCACCGATGGCCGTCAACTGCACCAGCAAGGGGGGGAACAGCGGTTCACCGGCCATCGCCTGGTTCAGCCCTTGCCCATTGGCCATGCCTTGGCGCAGCCGCAACACCGCCTGCTCGTg > 1:173813/1‑147 (MQ=255) cAGCATCTGG‑TCCAGC‑GTGCCGCTGGATTCACCGATGGCCGTCAACTGCACCAGCAAGGGGGGGAACAGCGGTTCACCGGCCATCGCCTGGTTCAGCCCTTGCCCATTGGCCATGCCTTGGCGCAGCCGCAACACCGCCTGCTCGTGc < 2:302086/148‑1 (MQ=255) cAGCATCTGG‑TCCAGC‑GTGCCGCTGGATTCACCGATGGCCGTCAACTGCACCAGCAAGGGGGGGAACAGCGGTTCACCGGCCATCGCCTGGTTCAGCCCTTGCCCATTGGCCATGCCTTGGCGCAGCCGCAACACCGCCTGCTCGTGc < 8:196247/148‑1 (MQ=255) cAGCATCTGG‑TCCAGC‑GTGCCGCTGGATTCACCGATGGCCGTCAACTGCACCAGCAAGGGGGGGAACAGCGGTTCACCGGCCATCGCCTGGTTCAGCCCTTGCCCATTGGCCATGCCTTGGCGCAGCCGCAACACCGCCTGCTCGTGc < 5:307216/148‑1 (MQ=255) cAGCATCTGG‑TCCAGC‑GTGCCGCTGGATTCACCGATGGCCGTCAACTGCACCAGCAAGGGGGGGAACAGCGGTTCACCGGCCATCGCCTGGTTCAGCCCTTGCCCATTGGCCATGCCTTGGCGCAGCCGCAACACCGCCTGCTCGTGCa < 4:289491/149‑1 (MQ=255) gCATCTGG‑TCCAGC‑GTGCCGCTGGATTCACCGATGGCCGTCAACTGCACCAGCAAGGGGGGGAACAGCGGTTCACCGGCCATCGCCTGGTTCAGCCCTTGCCCATTGGCCATGCCTTGGCGCAGCCGCAACACCGCCTGCTCGTGCAgg < 4:324274/149‑1 (MQ=255) cATCTGG‑TCCAGC‑GTGCCGCTGGATTCACCGATGGCCGTCAACTGCACCAGCAAGGGGGGGAACAGCGGTTCACCGGCCATCGCCTGGTTCAGCCCTTGCCCATTGGCCATGCCTTGGCGCAGCCGCAACACCGCCTGCTCGTGCAGGt < 2:277534/149‑1 (MQ=255) cATCTGG‑TCCAGC‑GTGCCGCTGGATTCACCGATGGCCGTCAACTGCACCAGCAAGGGGGGGAACAGCGGTTCACCGGCCATCGCCTGGTTCAGCCCTTGCCCATTGGCCATGCCTTGGCGCAGCCGCAACACCGCCTGCTCGTACAGGt < 1:143861/149‑1 (MQ=255) aTCTGG‑TCCAGC‑GTGCCGCTGGATTCACCGATGGCCGTCAACTGCACCAGCAAGGGGGGGAACAGCGGTTCACCGGCCATCGCCTGGTTCAGCCCTTGCCCATTGGCCATGCCTTGGCGCAGCCGCAACACCGCCTGCTCGTGCAGGTc < 8:255311/149‑1 (MQ=255) tCTGG‑TCCAGC‑GTGCCGCTGGATTCACCGATGGCCGTCAACTGCACCAGCAAGGGGGGGAACAGCGGTTCACCGGCCATCGCCTGGTTCAGCCCTTGCCCATTGGCCATGCCTTGGCGCAGCCGCAACACCGCCTGCTCGTGCAGGTCg > 5:59293/1‑149 (MQ=255) gtgaatCCAGC‑GGCACGCTGGATTCACCGATGGCCGTCAACTGCACCAGCAAGGGGGGAAACAGCGGTTCACCGGCCATCGCCTGGTTCAGCCCTTGCCCATTGGCCATGCCTTGGCGCAGCCGCAACACCGCCTGCTCGTGCAGGTCg > 4:162230/6‑149 (MQ=255) tGG‑TCCAGC‑GTGCCGCTGGATTCACCGATGGCCGTCAACTGCACCAGCAAGGGGGGGAACAGCGGTTCACCGGCCATCGCCTGGTTCAGCCCTTGCCCATTGGCCATGCCTTGGCGCAGCCGCAACACCGCCTGCTCGTGCAGGTCGc > 5:189720/1‑148 (MQ=255) g‑TCCAGC‑GTGCCGCTGGATTCACCGATGGCCGTCAACTGCACCAGCAAGGGGGGGAACAGCGGTTCACCGGCCATCGCCTGGTTCAGCCCTTGCCCATTGGCCATGCCTTGGCGCAGCCGCAACACCGCCTGCTCGTGCAGGTCg < 7:213411/145‑1 (MQ=255) g‑TCCAGC‑GTGCCGCTGGATTCACCGATGGCCGTCAACTGCACCAGCAAGGGGGGGAACAGCGGTTCACCGGCCATCGCCTGGTTCAGCCCTTGCCCATTGGCCATGCCTTGGCGCAGCCGCAACACCGCCTGCTCGTGCAGGTCGCCa < 1:267360/148‑1 (MQ=255) atCCAGC‑GGCACGCTGGATTCACCGATGGCCGTCAACTGCTCCAGCAAGGGGGGGAACAGCGGTTCACCGGCCATCGCCTGGTTCAGCCCTTGCCCATTGGCCATGCCTTGGCGCAGCCGCAACACCGCCTGCTCGTGCAGGTCGCCAc > 7:251197/2‑149 (MQ=255) tCCAGC‑GTGCCGCTGGATTCACCGATGGCCGTCAACTGCACCAGCAAGGGGGGGAACAGCGGTTCACCGGCCATCGCCTGGTTCAGCCCTTGCCCATTGGCCATGCCTTGGAGCAGCCGAAACACCGCCTGCTCTGGCAGGTCGACAcc > 7:337960/1‑149 (MQ=255) cAGC‑GTGCCGCTGGATTCACCGATGGCCGTCAACTGCACCAGCAAGGGGGGGAACAGCGGTTCACCGGCCATCGCCTGGTTCAGCCCTTGCCCATTGGCCATGCCTTGGCGCAGCCGCAACACCGCCTGCTCGTGCAGGTCGCCAccc < 4:294283/148‑1 (MQ=255) cAGC‑GTGCCGCTGGATTCACCGATGGCCGTCAACTGCACCAGCAAGGGGGGGAACAGCGGTTCACCGGCCATCGCCTGGTTCAGCCCTTGCCCATTGGCCATGCCTTGGCGCAGCCGCAACACCGCCTGCTCGTGCAGGTCGCCAccc < 6:204291/148‑1 (MQ=255) cAGC‑GTGCCGCTGGATTCACCGATGGCCGTCAACTGCACCAGCAAGGGGGGGAACAGCGGTTCACCGGCCATCGCCTGGTTCAGCCCTTGCCCATTGGCCATGCCTTGGCGCAGCCGCAACACCGCCTGCTCGTGCAGGTCGCCACCCg > 2:254710/1‑149 (MQ=255) c‑GTGCCGCTGGATTCACCGATGGCCGTCAACTGCACCAGCAAGGGGGGGAACAGCGGTTCACCGGCCATCGCCTGGTTCa > 5:141310/1‑80 (MQ=255) c‑GTGCCGCTGGATTCACCGATGGCCGTCAACTGCACCAGCAAGGGGGGGAACAGCGGTTCACCGGCCATCGCCTGGTTCa < 6:141310/80‑1 (MQ=255) tGCCGCTGTATTCACCGATGGCCGTCAACTGCACCAGCAAGGGGGGGAACAGCGGTTCACCGGCCATCGCCTGGTTCAGCCCTTGCCCATTGGCCATGCCTTGGCGCAGCCGCAACACCGCCTGCTCGTGCAGGTCGCCACCCGTTAcc < 4:220370/149‑1 (MQ=255) | GCAGGTACATCGCCACCACCAGGCCACCCACCAGCAAGCCCAGGATCAGCACGATGGCCGGCTCCAGCAGGCTGGTCAACTGATCCAGCGCCTGGCTGACCTGTTCCTCGTAATGGCTGGCGGCTTTTTCCAGCATCTGG‑TCCAGC‑GTGCCGCTGGATTCACCGATGGCCGTCAACTGCACCAGCAAGGGGGGGAACAGCGGTTCACCGGCCATCGCCTGGTTCAGCCCTTGCCCATTGGCCATGCCTTGGCGCAGCCGCAACACCGCCTGCTCGTGCAGGTCGCCACCCGTTACC > NC_002947/739447‑739742 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |