Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 852,171 A→T 9.8% Q317L (CAG→CTG)  hemA → glutamyl‑tRNA reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947852,1710AT 9.8% 224.6 / 11.6 81Q317L (CAG→CTG) hemAglutamyl‑tRNA reductase
Reads supporting (aligned to +/- strand):  ref base A (38/35);  new base T (6/2);  total (44/37)
Fisher's exact test for biased strand distribution p-value = 2.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01

GACACAAGCCCATCTTCATGGTCGACATCGCTGTACCTCGCGATATCGAAACCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTT‑CATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGCTCAGGGCCTATCGCCAGCAAAGTGAGCGCCTTCGCGACGAAGAACTGCAAAAGGCCTTGCGCCTGCTG  >  NC_002947/852024‑852316
                                                                                                                                                   |                                                                                                                                                  
gACACAAGCCCATCTTCATGGTCGACATCGCTGTACCTCGCGATATCGAAACCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAg                                                                                                                                                   <  5:299189/149‑1 (MQ=255)
 acacAAGCCCATCTTCATGGTCGACATCGCTGTACCTCGCGATATCGAAACCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAgg                                                                                                                                                  >  2:70848/1‑149 (MQ=255)
   acaAGCCCATCTTCATGGTCGACATCGCTGTACCTCGCGATATCGAAACCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAggcg                                                                                                                                                >  7:354676/1‑149 (MQ=255)
     aaGCCCATCTTCATGGTCGACATCGCTGTACCTCGCGATATCGAAACCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAggcggc                                                                                                                                              >  5:83573/1‑149 (MQ=255)
     aaGCCCATCTTCATGGTCGACATCGCTGTACCTCGCGATATCGAAACCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAg                                                                                                                                                   <  1:123945/144‑1 (MQ=255)
     aaGCCCATCTTCATGGTCGACATCGCTGTACCTCGCGATATCGAAACCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAg                                                                                                                                                   >  2:123945/1‑144 (MQ=255)
      aGCCCATCTTCATGGTCGACATCGCTGTACCTCGCGATATCGAAACCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGcc                                                                                                                                             <  5:187352/149‑1 (MQ=255)
       gCCCATCTTCATGGTCGACATCGCTGTACCTCGCGATATCGAAACCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCTGGGCCGCGCCCTggcggctc                                                                                                                                            <  5:286962/149‑3 (MQ=255)
         ccATATTCATGGTCGACATCGCTGTACCTCGCGATATCGAAACCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGa                                                                                                                                           >  5:38281/1‑148 (MQ=255)
           aTCTTCATGGTCGACATCGCTGTACCTCGCGATATCGAAACCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAaga                                                                                                                                        <  4:131020/149‑1 (MQ=255)
           aTCTTCATGGTCGACATCGCTGTACCTCGCGATATCGAAACCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAaga                                                                                                                                        <  6:290662/149‑1 (MQ=255)
              ttCATGGTCGACATCGCTGTACCTCGCGATATCGAAACCGAAGTCGGCGAGCTGGATGGTGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCt                                                                                                                                     >  4:309193/1‑149 (MQ=255)
              ttCATGGTCGACATCGCTGTACCTCGCGATATCGAAACCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCt                                                                                                                                     >  5:179982/1‑149 (MQ=255)
               tCATGGTCGACATCGCTGTACCTCGCGATATCGAAACCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTg                                                                                                                                    >  7:91657/1‑149 (MQ=255)
                cctGGTCGACATCGCTGTACCTCGCGATATCGAAACCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCt                                                                                                                                     <  4:77467/145‑1 (MQ=255)
                  tGGTCGACATCGCTGTACCTCGCGATATCGAAACCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTa                                                                                                                                 >  4:370887/1‑149 (MQ=255)
                   ggTCGACATCGCTGTACCTCGCGATATCGAAACCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGcc                                                                                                                                             <  5:220908/136‑1 (MQ=255)
                   ggTCGACATCGCTGTACCTCGCGATATCGAAACCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGcc                                                                                                                                             >  6:220908/1‑136 (MQ=255)
                     tCGACATCGCTGTACCTCGCGATATCGAAACCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATcc                                                                                                                              <  3:168638/149‑1 (MQ=255)
                     tCGACATCGCTGTACCTCGCGATATCGAAACCGAAGTCGGCGAGCTGGATGAAGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATcc                                                                                                                              >  1:289252/1‑149 (MQ=255)
                       gACATCGCTGTACCTCGCGATATCGAAACCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGt                                                                                                                            >  6:251050/1‑149 (MQ=255)
                        aCATCGCTGTACCTCGCGATATCGAAACCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTg                                                                                                                           <  1:70848/149‑1 (MQ=255)
                          aTCGCTGTACCTCGCGATATCGAAACCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTggg                                                                                                                         >  6:102276/1‑149 (MQ=255)
                                 tACCTCGCGATATCGAAACCGAAGTCGGCGAGCTGGATTATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCTGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGaa                                                                                                                  <  8:354676/149‑1 (MQ=255)
                                    ctctCGATATCGAAACCGAAGTCGGCGAGCTGGATGAGGTCTACCTGTACCCCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGAc                                                                                                               <  6:293947/149‑1 (MQ=255)
                                      cgcgATATCGAAACCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTGTCCGTGGGCGCCGAAGACtt                                                                                                             >  4:298568/1‑149 (MQ=255)
                                       gcgATATCGAAACCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACtt                                                                                                             <  2:315238/148‑1 (MQ=255)
                                       gcgATATCGAAACCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACtt                                                                                                             <  5:102276/148‑1 (MQ=255)
                                              cGAAACCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCTGGGCCGCGCCCTggcgg                                                                                                                                               <  6:270931/107‑1 (MQ=255)
                                              cGAAACCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCTGGGCCGCGCCCTggcgg                                                                                                                                               >  5:270931/1‑107 (MQ=255)
                                               gAAACCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTT‑CAtgctgc                                                                                                    <  6:38281/148‑1 (MQ=255)
                                               gAAACCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTT‑CATGctgcg                                                                                                   >  3:137207/1‑149 (MQ=255)
                                               gAAACCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGAAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTT‑CATGctgcg                                                                                                   >  4:40713/1‑149 (MQ=255)
                                                aaaCCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTT‑CATGctgcgc                                                                                                  >  8:242002/1‑149 (MQ=255)
                                                   ccGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTGTCCGTGGGCGCCGAAGACTT‑CATGctgcgcctg                                                                                               <  3:298568/149‑1 (MQ=255)
                                                      aaGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTT‑CATGctgcgcctgcgc                                                                                            <  4:11456/149‑1 (MQ=255)
                                                       aGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTT‑CATGctgcgcctgcgc                                                                                            >  7:152192/1‑148 (MQ=255)
                                                         tCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTT‑CATGCTGCGCCTGCGCGaa                                                                                         >  3:172093/1‑149 (MQ=255)
                                                           ggCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTT‑CATGCTGCGCCTGCGCGAAc                                                                                        <  5:362745/148‑1 (MQ=255)
                                                              gAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTT‑CATGCTGCGCCTGCGCGAACTGGc                                                                                    >  3:82431/1‑149 (MQ=255)
                                                               aGCTGGATGGTGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTT‑CATGCTGCGCCTGCGCGAACTGGCt                                                                                   <  3:309193/149‑1 (MQ=255)
                                                               aGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTT‑CATGCTGCGCCTGCGCGAACTGGCt                                                                                   <  1:39295/149‑1 (MQ=255)
                                                                gcttgttgaggTCTACCTGTACACCTGCGAGGACCTGCACGATGTCGTGGCGGAATACCTCTAGAGCCGACAGGGCGCGGCCGAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTT‑CATGCTGCGCCTGCGCGAACTGGCt                                                                                   <  2:348670/142‑1 (MQ=255)
                                                                         tgTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTT‑c                                                                                                           >  8:48063/1‑115 (MQ=255)
                                                                         tgTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTT‑c                                                                                                           <  7:48063/115‑1 (MQ=255)
                                                                         tgTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTT‑CATGCTGCGCCTGCGCGAACTGGCTGCAGTGGAc                                                                          <  5:251050/148‑1 (MQ=255)
                                                                           tCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTT‑CATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGt                                                                        <  2:289252/148‑1 (MQ=255)
                                                                           tCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTT‑CATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGt                                                                        >  6:349484/1‑148 (MQ=255)
                                                                            cTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGATGGTATCCGTCGGCGCCGAAGACTT‑CATGCTGCGCCTGCGAGAACTGGCTGCAGTGGACGTGc                                                                      >  2:188265/1‑149 (MQ=255)
                                                                             tACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTT‑CATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGCt                                                                     >  1:1353/1‑149 (MQ=255)
                                                                             tACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGCTATCCGTGGGCGCCGAAGACTT‑CATGCTGAGCCTGAGCGACCTGGCTGCAGTGGACTTgat                                                                     >  8:97023/1‑147 (MQ=255)
                                                                              aCCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTT‑CATGCTGCGCCTGCGCGAACTGGCTACAGTGGACGTGCt                                                                     >  2:154972/1‑148 (MQ=255)
                                                                               ccTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTT‑CATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGc                                                                      <  3:370887/146‑1 (MQ=255)
                                                                                cTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGTCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGAGCCGAAGACTT‑CATGCTGCGCCTCCGCGCACTGGCTGCAGTGGACCTGCTCAg                                                                  >  8:308897/1‑149 (MQ=255)
                                                                                  gTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTT‑CATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGCTCAggg                                                                >  2:42151/1‑149 (MQ=255)
                                                                                   tACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTT‑CATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGCTCAggg                                                                <  8:152192/148‑1 (MQ=255)
                                                                                   tACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTT‑CATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGCTCAgg                                                                 <  6:83573/147‑1 (MQ=255)
                                                                                      acCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTT‑CATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGCTCAGGGCCTa                                                            >  6:355026/1‑149 (MQ=255)
                                                                                        cGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTT‑CATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGCTCAGGGCCTATc                                                          <  3:363298/149‑1 (MQ=255)
                                                                                        cGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTT‑CATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGCTCAGGGCCTATc                                                          >  1:166887/1‑149 (MQ=255)
                                                                                            gacgacCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTT‑CATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGCTCAGGGCCTATCGCCa                                                      <  4:137207/149‑1 (MQ=255)
                                                                                             acgacCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTT‑CATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGCTCAGGGCCTATCGCCAg                                                     >  3:333283/1‑149 (MQ=255)
                                                                                                  cTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTAGCCGTGGGCGCCGAAGCCTT‑CATGCTGCGCATGCGCGAACTGGCTGCAGTGGACGTGCTCAGGGCCTATCGCCAGCAAAg                                                >  6:121649/1‑149 (MQ=255)
                                                                                                     cACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTT‑CATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGCTCAGGGCCTATCGCCAGCAAAGTg                                              <  6:138377/148‑1 (MQ=255)
                                                                                                       cGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTT‑CATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGCTCAGGGCCTATCGCCAGCAAAGTGAgc                                           <  2:1353/149‑1 (MQ=255)
                                                                                                          tGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTT‑CATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGCTCAGGGCCTATCGCCAGCAAAGTGAGCGcc                                        >  5:210985/1‑149 (MQ=255)
                                                                                                                ggCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTT‑CATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGCTCAGGGCCTATCGCCAGCAAAGTGAGCGCCTTcgc                                   >  1:7210/1‑148 (MQ=255)
                                                                                                                  cGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTT‑CATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGCTCAGGGCCTATCGCCAGCAAAGTGAGCGCCTTCGcgac                                <  7:242002/149‑1 (MQ=255)
                                                                                                                  cGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTT‑CATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGCTCAGGGCCTATCGCCAGCAAAGTGAGCGCCTTCGcgac                                <  5:355026/149‑1 (MQ=255)
                                                                                                                    gAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTT‑CATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGCTCAGGGCCTATCGCCAGCAAAGTGAGCGCCTTCGcgacg                               >  8:103320/1‑148 (MQ=255)
                                                                                                                     aaaaCCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTT‑CATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGCTCAGGGCCTATCGCCAGCAAAGTGAGCGCCTTCGCGACgaa                             <  8:91657/149‑1 (MQ=255)
                                                                                                                           tcttcggccgccTGGGCCGCGCCCTGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTT‑CATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGCTCAGGGCCTATCGCCAGCAAAGTGAGCGCCTTCGCGACGAAGAACTg                       >  7:171386/7‑149 (MQ=255)
                                                                                                                                 gccgccTGGGCCGCGCCCTGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTT‑CATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGCTCAGGGCCTATCGCCAGCAAAGTGAGCGCCTTCGCGACGAAGAACTGCAAAAg                 >  3:180277/1‑149 (MQ=255)
                                                                                                                                 gccgccTGGGCCGCGCCCTGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTT‑CATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGCTCAGGGCCTATCGCCAGCAAAGTGAGCGCCTTCGCGACGAAGAACTGCAAAAg                 >  6:103910/1‑149 (MQ=255)
                                                                                                                                 gccgccTGGGCCGCGCCCTGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTT‑CATGATGCGACTGCGCGAACTGGCTGCAGTGGACGTGCTCAGGGCCTATCGCCAGCAAAGTGAGCGCCTTCGCGACGAAGAACTGCAAAAg                 >  2:120340/1‑149 (MQ=255)
                                                                                                                                 gccgccTGGGCCGCGCCCTGGCGGCCGAAGAGCTGGTATCAGTGGGCGCCGAAGACTT‑CATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGCTCAGGGCCTATCGCCAGCAAAGTGAGCGCCTTCGCGACGAAGAACTTCAAAAg                 >  2:115654/1‑149 (MQ=255)
                                                                                                                                    gccAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTT‑CATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGCTCAGGGCCTATCGCCAGCAAAGTGAGCGCCTTCGCGACGAAGAACTGCAAAAGGcc              >  3:249577/1‑149 (MQ=255)
                                                                                                                                      cAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTT‑CATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGCTCAGGGCCTATCGCCAGCAAAGTGAGCGCCTTCGCGACGAAGAACTGCAAAAGGCCtt            >  2:102270/1‑149 (MQ=255)
                                                                                                                                          gcgcGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTT‑CATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGCTCAGGGCCTATCGCCAGCAAAGTGAGCGCCTTCGCGACGAAGAACTGCAAAAGGCCTTgcgc        <  1:42151/149‑1 (MQ=255)
                                                                                                                                                cccAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTT‑CATGCTGCGCCTGCGCGAACTGGCTACAGTGGACGTGCTCAGGGCCTATCGCCAGCAAAGTGAGCGCCTTCGCGACGAAGAACTGCAAAAGGCCTTGCGCctgctg  <  1:154972/149‑1 (MQ=255)
                                                                                                                                                 ccAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTTGCATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGCTCAGGGCCTATCGCCAGCAAAGTGAGCGCCTTCGCGACGAAGAACTGCAAAAGGCCTTGCGCctgctg  >  1:72437/1‑149 (MQ=255)
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GACACAAGCCCATCTTCATGGTCGACATCGCTGTACCTCGCGATATCGAAACCGAAGTCGGCGAGCTGGATGATGTCTACCTGTACACCGTCGACGACCTGCACGATGTAGTGGCGGAAAACCTCAAGAGCCGCCAGGGCGCGGCCCAGGCGGCCGAAGAGCTGGTATCCGTGGGCGCCGAAGACTT‑CATGCTGCGCCTGCGCGAACTGGCTGCAGTGGACGTGCTCAGGGCCTATCGCCAGCAAAGTGAGCGCCTTCGCGACGAAGAACTGCAAAAGGCCTTGCGCCTGCTG  >  NC_002947/852024‑852316

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑