Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 860,699 T→A 7.1% intergenic (‑28/+76) PP_0741 ← / ← PP_0742 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947860,6990TA 7.1% 188.5 / 3.0 65intergenic (‑28/+76)PP_0741/PP_0742hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (27/33);  new base A (3/2);  total (30/35)
Fisher's exact test for biased strand distribution p-value = 6.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

TGTTTACGGCACAGGTCGGAGGCCTTGTGGCCGCCGTTGTAGCGCACGCTGTGCCCGGTCAGGCAGGCGCTGATGGCGATACGCGGCTTGCTGTGGGCGGAAGGGTCGTGCATGGCTGCCTCCGAAAATAAACCTGTACGTGACTCGAAACTTGTACAGGTTTATTTCAGCACAGCGCTGAAGTTATGCAAACTAGTATTTTTGTATAGTTCGGCATCACTCAAGGTGTTCGAGCAAGCGCCGCGCTGCTTCCTGGCCACTGAGCCATGCGCCCTCGACTCGGCC  >  NC_002947/860559‑860843
                                                                                                                                            |                                                                                                                                                
tGTTTACGGCACAGGTCGGAGGCCTTGTGGCCGCCGTTGTAGCGCACGCTGTGCCCGGTCAGGCAGGCGCTGATGGCGATACGCGGCTTGCTGTGGGCGGAAGGGTCGTGCATGGCTGCCTCCGAAAATAAACCTGTACGTGACTCGa                                                                                                                                           >  5:308809/1‑148 (MQ=255)
     aCGGCACAGGTCGGAGGCCTTGTGGCCGCCGTTGTAGCGCACGCTGTGCCCGGTCAGGCAGGCGCTGATGGCGATACGCGGCTTGCTGTGGGCGGAAGGGTCGTGCATGGCTGCCTCCGAAAATAAACCTGTACGTGACTCGAAACtt                                                                                                                                      <  8:314942/148‑1 (MQ=255)
     aCGGCACAGGTCGGAGGCCTTGTGGCCGCCGTTGTAGCGCACGCTGTGCCCGGTCAGGCAGGCGCTGATGGCGATACGCGGCTTGCTGTGGGCGGAAGGGTCGTGCATGGCTGCCTCCGAAAATAAACCTGTACGTGACTCGAAACtt                                                                                                                                      <  8:234718/148‑1 (MQ=255)
              gTCGGAGGCCTTGTGGCCGCCGTTGTAGCGCACGCTGTGCCCGGTCAGGCAGGCGCTGATGGCGATACGCGGCTTGCTGTGGGCGGAAGGGTCGTGCATGGCTGCCTCCGAAAATAAACCTGTACGTGACTCGAAACTTGTACAGGttt                                                                                                                            <  5:372449/149‑1 (MQ=255)
                 ggAGGCCTTGTGGCCGCCGTTGTAGCGCACGCTGTGCCCGGTCAGGCAGGCGCTGATGGCGATACGCGGCTTGCTGTGGGCGGAAGGGTCGTGCATGGCTGCCTCCGAAAATAAACCTGTACGTGACTCGAAACTTGTACAGGTTTAtt                                                                                                                         >  6:184851/1‑149 (MQ=255)
                 ggAGGCCTTGTGGCCGACGTTGTAGCGCACGCTGTGCCCGGTCAGGCAGGCGCTGATGGCGATACGCGGCTTGCTGTGGGCGGAAGGGTCGTGCATGGCTGCCTCCGAAAATAAACCTGTACGTGACTCGAAACTTGTACAGGTTTAtt                                                                                                                         >  2:168565/1‑149 (MQ=255)
                    ggCCTTGTGGCCGCCGTTGTAGCGCACGCTGTGCCCGGTCAGGCAGGCGCTGATGGCGATACGCGGCTTGCTGTGGGCGGAAGGGTCGTGCATGGCTGCCTCCGAAAATAAACCTGTACGTGACTCGAAACTTGTACAGGTTTATTTCa                                                                                                                      <  2:288121/149‑1 (MQ=255)
                     gCCTTGTGGCCGCCGTTGTAGCGCACGCTGTGCCCGGTCAGGCAGGCGCTGATGGCGATACGCGGCTTGCTGTGGGCGTAAGGGTCGTGCATGGCTGCCTCCGAAAATAAACCTGTACGTGACTCGAAAGTTGTACAGGTTTATTTCa                                                                                                                      <  4:110305/148‑1 (MQ=255)
                     gCCTTGTGGCCGCCGTTGTAGCGCACGCTGTGCCCGGTCAGGCAGGCGCTGATGGCGATACGCGGCTTGCTGTGGGCGGAAGGGTCGTGCATGGCTGCCTCCGAAAATAAACCTGTACAAGTTTCGAGTCACGTACAGGTTTAttttcg                                                                                                                     <  2:269975/149‑4 (MQ=37)
                       cTTGTGGCCGCCGTTGTAGCGCACGCTGTGCCCGGTCAGGCAGGCGCTGATGGCGATACGCGGCTTGCTGTGGGCGGAAGGGTCGTGCATGGCTGCCTCCGAAAATAAACCTGTACGTGACTCGAAACTTGTACAGGTTTATTTCAGca                                                                                                                   >  6:214915/1‑149 (MQ=255)
                        ttGTGGCCGCCGTTGTAGCGCACGCTGTGCCCGGTCAGGCAGGCGCTGATGGCGATACGCGGCTTGCTGTGGGCGGAAGGGTCGTGCATGGCTGCCTCCGAAAATAAACCTGTACGTGACTCGAAACTTGTACAGGTTTATTTCAGca                                                                                                                   >  6:11378/1‑148 (MQ=255)
                             gccgccGTTGTAGCGCACGCTGTGCCCGGTCAGGCAGGCGCTGATGGCGATACGCGGCTTGCTGTGGGCGGAAGGGTCGTGCATGGCTGCCTCCGAAAATAAACCTGTACGTGACTCGAAACTTGTACAGGTTTATTTCAGCACAgcgc                                                                                                             >  2:210782/1‑149 (MQ=255)
                                   gTTGTAGCGCACGCTGTGCCCGGTCAGGCAGGCGCTGATGGCGATACGCGGCTTGCTGTGGGCGGAAGGGTCGTGCATGGCTGCCTCCGAAAATAAACCTGTACGTGACTCGAAACTTGTACAGGTTTATTTCAGCACAGCGCTGAAGt                                                                                                       <  1:82237/149‑1 (MQ=255)
                                   gTTGTAGCGCACGCTGTGCCCGGTCAGGCAGGCGCTGATGGCGATACGCGGCTTGCTGTGGGCGGAAGGGTCGTGCATGGCTGCCTCCGAAAATAAACCTGTACGTGACTCGAAACTTGTACAGGTTTATTTCAGCACAGCGCTGAAGt                                                                                                       >  6:333883/1‑149 (MQ=255)
                                      gTAGCGCACGCTGTGCCCGGTCAGGCAGGCGCTGATGGCGATACGCGGCTTGCTGTGGGCGGAAGGGTCGTGCATGGCTGCCTCCGAAAATAAACCTGTACGTGACTCGAAACTTGTACAGGTTTATTTCAGCACAGCGCTGAAGTTa                                                                                                     <  5:214915/148‑1 (MQ=255)
                                       tAGCGCACGCTGTGCCCGGTCAGGCAGGCGCTGATGGCGATACGCGGCTTGCTGTGGGCGGAAGGGTCGTGCATGGCTGCCTCCGAAAATAAACCTGTACGTGACTCGAAACTTGTACAGGTTTATTTCAGCACAGCGCTGAAGTTAt                                                                                                    <  8:94089/148‑1 (MQ=255)
                                       tAGCGCACGCTGTGCCCGGTCAGGCAGGCGCTGATGGCGATACGCGGCTTGCTGTGGGCGGAAGGGTCGTGCATGGCTGCCTCCGAAAATAAACCTGTACGTGACTCGAAACTTGTACAGGTTTATTTCAGCACAGCGCTGAAGTTATg                                                                                                   <  8:293750/149‑1 (MQ=255)
                                         gcgcACGCTGTGCCCGGTCAGGCAGGCGCTGATGGCGATACGCGGCTTGCTGTGGGCGGAAGGGTCGTGCATGGCTGCCTCCGAAAATAAACCTGTACGTGACTCGAAACTTGTACAGGTTTATTTCAGCACAGCGCTGAAGTTATGc                                                                                                  <  6:143323/148‑1 (MQ=255)
                                           gcACGCTGTGCCCGGTCAGGCAGGCGCTGATGGCGATACGCGGCTTGCTGTGGGCGGAAGGGTCGTGCATGGCTGCCTCCGAAAATAAACCTGTACGTGACTCGAAACTTGTACAGGTTTATTTCAGCACAGCGCTGAAGTTATGCaaa                                                                                               >  1:165372/1‑149 (MQ=255)
                                            cACGCTGTGCCCGGTCAGGCAGGCGCTGATGGCGATACGCGGCTTGCTGTGGGCGGAAGGGTCGTGCATGGCTGCCTCCGAAAATAAACCTGTACGTGACTCGAAACTTGTACAGGTTTATTTCAGCACAGCGCTGAAGTTATGCAAAc                                                                                              >  7:143068/1‑149 (MQ=255)
                                              cGCTGTGCCCGGTCAGGCAGGCGCTGATGGCGATACGCGGCTTGCTGTGGGCGGAAGGGTCGTGCATGGCTGCCTCCGAAAATACACCTGTACGTGACTCGAAACTTGTACAGGTTTATTTCAGCACAGCGCTGAAGTTATGCAAACTa                                                                                            <  2:231600/149‑1 (MQ=255)
                                                   tgCCCGGTCAGGCAGGCGCTGATGGCGATACGCGGCTTGCTGTGGGCGGAAGGGTCGTGCATGTCTGCCTCCGAAAATAAACCTGTACGTGACTCGAAACTTGTACAGGTTTATTTCAGCACAGCGCTGAAGTTATGCAAACTAGTa                                                                                         <  7:239584/147‑1 (MQ=255)
                                                   tgCCCGGTCAGGCAGGCGCTGATGGCGATACGCGGCTTGCCGTGGGCGGAAGGGTCGTGCATGGCTGCCTCCGAAAATAAACCTGTACGTGACTCGAAACTTGTACAGGTTTATTTCAGCACAGCGCTGAAGTTATGCAAACTAGTa                                                                                         <  1:363800/147‑1 (MQ=255)
                                                     cccGGTCAGGCAGGCGCTGATGGCGATACGCGGCTTGCTGTGGGCGGAAGGGTCGTGCATGGCTGCCTCCGAAAATAAACCTGTACGTGACTCGAAACTTGTACAGGTTTATTTCAGCACAGCGCTGAAGTTATGCAAACTa                                                                                            <  4:8083/142‑1 (MQ=255)
                                                     cccGGTCAGGCAGGCGCTGATGGCGATACGCGGCTTGCTGTGGGCGGAAGGGTCGTGCATGGCTGCCTCCGAAAATAAACCTGTACGTGACTCGAAACTTGTACAGGTTTATTTCAGCACAGCGCTGAAGTTATGCAAACTa                                                                                            >  3:8083/1‑142 (MQ=255)
                                                     cccGGTCAGGCAGGCGCTGATGGCGATACGCGGCTTGCTGTGGGCGGAAGGGTCGTGCATGGCTGCCTCCGAAAATAAACCTGTACAAGTTTCGAGTCACGTACAGGTTTAttttc                                                                                                                      >  2:265573/1‑114 (MQ=25)
                                                     cccGGTCAGGCAGGCGCTGATGGCGATACGCGGCTTGCTGTGGGCGGAAGGGTCGTGCATGGCTGCCTCCCAAAATAAACCTGTACAAGTTTCGAGTCACGTACAGGTTTAttttc                                                                                                                      <  1:265573/116‑3 (MQ=21)
                                                      ccGGTCAGGCAGGCGCTGATGGCGATACGCGGCTTGCTGTGGGCGGAAGGGTCGTGCATGGCTGCCTCCGAAAATAAACCTGTACGTGACTCGAAACTTGTACAGGTTTATTTCAGCACAGCGCTGAAGTTATGCAAACTAGTAttttt                                                                                    <  5:333883/149‑1 (MQ=255)
                                                        ggTCAGGCAGGCGCTGATGGCGATACGCGGCTTGCTGTGGGCGGAAGGGTCGTGCATGGCTGCCTCCGAAAATAAACCTGTACGTGACTCGAAACTTGTACAGGTTTATTTCAGCACAGCGCTGAAGTTATGCAAACTAGTATTTTTg                                                                                   >  5:164470/1‑148 (MQ=255)
                                                        ggTCAGGCAGGCGCTGATGGCGATACGCGGCTTGCTGTGGGCGGAAGGGTCGTGCATGGCTGCCTCCGAAAATAAACCTGTACGTGACTCGAAACTTGTACAGGTTTATTTCAGCACAGCGCTGAAGTTATGCAAACTAGTATTTTTg                                                                                   >  1:61406/1‑148 (MQ=255)
                                                        ggTCAGGCAGGCGCTGATGGCGATACGCGGCTTGCTGTGGGCGGAAGGGTCGTGCATGGCTGCCTCCGAAAATAAACCTGTACGTGACTCGAAACTTGTACAGGTTTATTTCAGCACAGCGCTGAAGTTATGCAAACTAGTATTTTTGt                                                                                  <  7:54648/149‑1 (MQ=255)
                                                            aggcaggcGCTGATGGCGATACGCGGCTTGCTGTGGGCGGAAGGGTCGTGCATGGCTGCCTCCGAAAATAAACCTGTACGTGACTCGAAACTTGTACAGGTTTATTTCAGCACAGCGCTGAAGTTATGCAAACTAGTATTTTTGtata                                                                               <  5:184851/148‑1 (MQ=255)
                                                             ggcaggcGCTGATGGCGATACGCGGCTTGCTGTGGGCGGAAGGGTCGTGCATGGCTGCCTCCGAAAATAAACCTGTACGTGACTCGAAACTTGTACAGGTTTATTTCAGGACAGCGCTGAAGTTATGCAAACTAGTATTTTTGTATAGt                                                                             <  1:168565/149‑1 (MQ=255)
                                                              gcaggcGCTGATGGCGATACGCGGCTTGCTGTGGGCGGAAGGGTCGTGCATGGCTGCCTCCGAAAATAAACCTGTACGTGACTCGAAACTTGTACAGGTTTATTTCAGCACAGCGCTGAAGTTATGCAAACTAGTATTTTTGTATAGt                                                                             >  4:88130/1‑148 (MQ=255)
                                                                aggcGCTGATGGCGATACGCGGCTTGCTGTGGGCGGAAGGGTCGTGCATGGCTGCCTCCGAAAATAAACCTGTACGTGACTCGAAACTTGTACAGGTTTATTTCAGCACAGCGCTGAAGTTATGCAAACTAGTATTTTTGtat                                                                                >  3:176984/1‑143 (MQ=255)
                                                                aggcGCTGATGGCGATACGCGGCTTGCTGTGGGCGGAAGGGTCGTGCATGGCTGCCTCCGAAAATAAACCTGTACGTGACTCGAAACTTGTACAGGTTTATTTCAGCACAGCGCTGAAGTTATGCAAACTAGTATTTTTGtat                                                                                <  4:176984/143‑1 (MQ=255)
                                                                   cccTGATGGCGATCCGCGGCTGGCTGGTGGCGGAAGTGTCGGGCATGGCTGCCGCCGAAAATACCCCTGTACGTGACTCGAAACTTGTACAGGTTTATTTCAGCACAGCGCTGAAGTTATGCAAACTAGTATTTTTGTATAGTTCGGCa                                                                       <  8:255977/147‑1 (MQ=255)
                                                                    gcTGATGTCGATACGCGGCTTGCTGTGGGCGGAATGGTCGTGCATGGCTGCCTCCGAAAATAAACCTGTACGTGACTGGATACTTGTACAGGTTTATTTCAGCACAGCGCTGAAGTTATGCAAACTAGTATTTTTGTATAGTTCGGCAt                                                                      <  5:10000/149‑1 (MQ=255)
                                                                            cGATACGCGGCTTGCTGTGGGCGGAAGGGTCGTGCATGGCTGCCTCCGAAAATAAACCTGTACGTGACTCGAAACTTGTACAGGTTTATTTCAGCACAGCGCTGAAGTTATGCAAACTAGTATTTTTGTATAGTTCGGCATCACTCAAg                                                              <  6:308809/149‑1 (MQ=255)
                                                                            cGATACGCGGCTTGCTGTGGGCGGAAGGGTCGTGCATGGCTGCCTCCGAAAATAAACCTGTACGTGACTCGAAACTTGTACAGGTTTATTTCAGCACAGCGCTGAAGTTATGCAAACTAGTATTTTTGTATAGTTCGGCATCACTCAAg                                                              <  1:344556/149‑1 (MQ=255)
                                                                                  gcgGCTTGCTGTGGGCGGAAGGGTCGTGCATGGCTGCCTCCGAAAATAAACCTGTACGTGACTCGAAACTTGTACAGGTTTATTTCAGCACAGCGCTGAAGTTATGCAAACTAGTATTTTTGTATAGTTCGGCATCa                                                                    >  7:260098/1‑137 (MQ=255)
                                                                                  gcgGCTTGCTGTGGGCGGAAGGGTCGTGCATGGCTGCCTCCGAAAATAAACCTGTACGTGACTCGAAACTTGTACAGGTTTATTTCAGCACAGCGCTGAAGTTATGCAAACTAGTATTTTTGTATAGTTCGGCATCa                                                                    <  8:260098/137‑1 (MQ=255)
                                                                                      cTTGCTGTGGGCGGAAGGGTCGTGCATGGCTGCCTCCGAAAATAAACCTGTACGTGACTCGAAACTTGTACAGGTTTATTTCAGCACAGCGCTGAAGTTATGCAAACTAGTATTTTTGTATAGTTCGGCATCACTCAAGGTGTTCGAGc                                                    <  2:165372/149‑1 (MQ=255)
                                                                                       ttGCTGTGGGCGGAAGGGTCGTGCATGGCTGCCTCCGAAAATAAACCTGTACGTGACTCGAAACTTGTACAGGTTTATTTCAGCACAGCGCTGAAGTTATGCAAACTAGTATTTTTGTATAGTTCGGCATCACTCAAGGTGTTCGAGc                                                    >  8:233131/1‑148 (MQ=255)
                                                                                                gCGGAAGGGTCGTGCATGGCTGCCTCCGAAAATAAACCTGTACGTGACTCGAAACTTGTACAGGTTTATTTCAGCACAGCGCTGAAGTTATGCAAACTAGTATTTTTGTATAGTTCGGCATCACTCAAGGTGTTCGAGCAAGCGCcgcg                                          >  4:122112/1‑149 (MQ=255)
                                                                                                gCGGAAGGGTCGTGCATGGCTGCCTCCGAAAATAAACCTGTACGTGACTCGAAACTTGTACAGGTTTATTTCAGCACAGCGCTGAAGTTATGCAAACTAGTATTTTTGTATAGTTCGGCATCACTCAAGGTGTTCGAGCAAGCGCcgcg                                          >  3:329793/1‑149 (MQ=255)
                                                                                                gCGGAAGGGTCGTGCATGGCTGCCTCCGAAAATAAACCTGTACGTGACTCGAAACTTGTACAGGTTTATTTCAGCACAGCGCTGAAGTTATGCAAACTAGTATTTTTGTATAGTTCGGCATCACTCAAGGTGTTCGAGCAAGCGCcgcg                                          >  5:203618/1‑149 (MQ=255)
                                                                                                       ggTCGTGCATGGCTGCCTCCGAAAATAAACCTGTACGTGACTCGAAACTTGTACAGGTTTATTTCAGCACAGCGCTGAAGTTATGCAAACTAGTATTTTTGTATAGTTCGGCATCACTCAAGGTGTTCGAGCAAGCGCCGCGCTGCTTc                                   >  5:208972/1‑149 (MQ=255)
                                                                                                       ggTCGTGCATGGCTGCCTCCGAAAATAAACCTGTACGTGACTCGAAACTTGTACAGGTTTATTTCAGCACAGCGCTGAAGTTATGCAAACTAGTATTTTTGTATAGTTCGGCATCACTCAAGGTGTTCGAGCAAGCGCCGCGCTGCTTc                                   <  3:88130/149‑1 (MQ=255)
                                                                                                           gTGCATGGCTGCCTCCGAAAATAAACCTGTACGTGACTCGAAACTTGTACAGGTTTATTTCAGCACAGCGCTGAAGTTATGCAAACTAGTATTTTTGTATAGTTCGGCATCACTCAAGGTGTTCGAGCAAGCGCCGCGCTGCTTCCTg                                <  1:198473/148‑1 (MQ=255)
                                                                                                              cATGGCTGCCTCCGAAAATAAACCTGTACGTGACTCGAAAGTTGTACAGGTTTATTTCAGCACAGCGCTGAAGTTATGCAAACTAGTATTTTTGTATAGTTCGGCATCACTCAAGGTGTTCGAGCAAGCGCCGCGCTGCTTCCTGGCCa                            >  2:72524/1‑149 (MQ=255)
                                                                                                              cATGGCTGCCTCCGAAAATAAACCTGTACGTGACTCGAAACTTGTACAGGTTTATTTCAGCACAGCGCTGAAGTTATGCAAACTAGTATTTTTGTATAGTTCGGCATCACTCAAGGTGTTCGAGCAAGCGCCGCGCTGCTTCCTGGCCa                            <  6:203618/149‑1 (MQ=255)
                                                                                                              cATGGCTGCCTCCGAAAATAAACCTGTACGTGACTCGAAACTTGTACAGGTTTATTTCAGCACAGCGCTGAAGTTATGCAAACTAGTATTTTTGTATAGTTCGGCATCACTCAAGGTGTTCGAGCAAGCGCCGCGCTGCTTCCTGGCCa                            <  4:329793/149‑1 (MQ=255)
                                                                                                                 ggCTGCCTCCGAAAATAAACCTGTACGTGACTCGAAACTTGTACAGGTTTATTTCAGCACAGCGCTGAAGTTATGCAAACTAGTATTTTTGTATAGTTCGGCATCAc                                                                   >  4:325143/1‑107 (MQ=255)
                                                                                                                 ggCTGCCTCCGAAAATAAACCTGTACGTGACTCGAAACTTGTACAGGTTTATTTCAGCACAGCGCTGAAGTTATGCAAACTAGTATTTTTGTATAGTTCGGCATCAc                                                                   <  3:325143/107‑1 (MQ=255)
                                                                                                                  gCTGCCTCCGAAAATAAACCTGTACGTGACTCGAAACTTGTACAGGTTTATTTCAGCACAGCGCTGAAGTTATGCAAACTAGTATTTTTGTATAGTTCGGCATCACTCAAGGTGTTCGAGCAAGCGCCGCGCTGCTTCCTGGCCACTGa                        <  5:11378/149‑1 (MQ=255)
                                                                                                                       cTCCGAAAATAAACCTGTACGTTACTCGAAACTTGTACAGGTTTATTTCAGCACAGCGCTGAAGTTATGCAAACTAGTATTTTTGTATAGTTCGGCATCACTCAAGGTGTTCGAGCAAGCGCCGCGCTGCTTCCTGGCCACTGAGCCAt                   <  3:122112/149‑1 (MQ=255)
                                                                                                                       cTCCGAAAATAAACCTCTACGTGACTCGAAACTTGTACAGGTTTATTTCAGCACAGCGCTGAAGTTATGCAAACTACTATTTTTGTATAGTTCGGCATCACTCAAGGTGTTCGAGCAAGCGCCGCGCTGCTTCCTGGCCACTGAGCCAt                   >  5:114574/1‑149 (MQ=255)
                                                                                                                        tcagAAAATAAACCTGTACGTGACTCGAAACTTGTACAGGTTTATTTCAGCACAGCGCTGAAGTTATGCAAACTAGTATTTTTGTATAGTTCGGCATCACTCAAGGTGTTCGAGCAAGCGCCGCGCTGCTTCATGGCCACTGAGCCATg                  >  6:286547/4‑149 (MQ=255)
                                                                                                                        tCCGAAAATAAACCTGTACGTGACTCGAAACTTGTACAGGTTTATTTCAGCACAGCGCTGAAGTTATGCAAACTAGTATTTTTGTATAGTTCGGCATCACTCAAGGTGTTCGAGCAAGCGCCGCGCTGCTTCCTGGCCACTGAGCCATg                  >  3:247501/1‑149 (MQ=255)
                                                                                                                           tgaaaTAAACCTGTACAAGTTTCGAGTCACGTACAGGTTTATTTCAGCACAGCGCTGAAGTTATGCAAACTAGTATTTTTGTATAGTTCGGCATCACTCAAGGTGTTCGAGCAAGCGCCGCGCTGCTTCCTGGCCACTGAGCCATgcgc               >  3:146430/3‑149 (MQ=37)
                                                                                                                               aTAAACCTGTACAAGTTTCGAGTCACGTACAGGTTTATTTCAGCACAGCGCTGAAGTTATGCAAACTAGTATTTTTGTATAGTTCGGCATCACTCAAGGTGTTCGAGCAAGCGCCGCGCTGCTTCCTGGCCACTGAGCCATGCGCCCTc           >  5:333330/1‑149 (MQ=38)
                                                                                                                                    ccTGTACGTGACTCGAAACTTGTACAGGTTTATTTCAGCACAGCGCTGAAGTTATGCAAACTAGTATTTTTGTATAGTTCGGCATCACTCAAGGTGTTCGAGCAAGCGCCGCGCTGCTTCCTGGCCACTGAGCCATGCGCCCTCGACTc      >  2:103287/1‑149 (MQ=255)
                                                                                                                                     cTGTACGTGACTCGAAACTTGTACAGGTTTATTTCAGCACAGCGCTGAAGTTATGCAAACTAGTATTTTTGTATAGTTCGGCATCACTCAAGGTGTTCGAGCAAGCGCCGCGCTGCTTCCTGGCCACTGAGCCATGCGCCCTCGACTCg     <  7:233131/149‑1 (MQ=255)
                                                                                                                                        tACGTGACTCGAAACTTGTACAGGTTTATTTCAGCACAGCGCTGAAGTTATGCAAACTAGTATTTTTGTATAGTTCGGCATCACTCAAGGTGTTCGAGCAAGCGCCGCGCTGCTTCCTGGCCACTGAGCCATGCGCCCTCGACTCGGcc  >  4:316263/1‑149 (MQ=255)
                                                                                                                                            |                                                                                                                                                
TGTTTACGGCACAGGTCGGAGGCCTTGTGGCCGCCGTTGTAGCGCACGCTGTGCCCGGTCAGGCAGGCGCTGATGGCGATACGCGGCTTGCTGTGGGCGGAAGGGTCGTGCATGGCTGCCTCCGAAAATAAACCTGTACGTGACTCGAAACTTGTACAGGTTTATTTCAGCACAGCGCTGAAGTTATGCAAACTAGTATTTTTGTATAGTTCGGCATCACTCAAGGTGTTCGAGCAAGCGCCGCGCTGCTTCCTGGCCACTGAGCCATGCGCCCTCGACTCGGCC  >  NC_002947/860559‑860843

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑