Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 868,515 T→C 7.1% K358R (AAG→AGG)  mqo‑I ← malate:quinone oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947868,5150TC 7.1% 203.9 / 4.5 70K358R (AAG→AGG) mqo‑Imalate:quinone oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (28/37);  new base C (2/3);  total (30/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.69e-01

CGCCTTTTTTCGGGTCTTTCTTGATGATCTGCACGCGCTGACCCGCCACTTCCAGGCGCCAGTCCTCGGCCTTGGCCTGCGGATAGAAACGACGCAGGGCTTCCAGACGCTGCTCCATCGACTGCATCACTTCGCTGACCAGGTACTTGGTCAGGTCCATGTTGTCGCGGGCCACGGCCAGCATCGGGCCGATGTTGCCCATGCGCACCGACAGCGGCAGGTCCATCAGCGAGCCGTGCTTGAGGAACTTGGTAGTGAAACCGGCATACGGACCGAACAGCAGCGAAGTCTTGC  >  NC_002947/868369‑868662
                                                                                                                                                  |                                                                                                                                                   
cgccTTTTTTCGGGTCTTTCTTGATGATCTGCACGCGCTGACCCGCCACTTCCAGGCGCCAGTCCTCGGCCTTGGCCTGCGGATAGAAACGACGCAGGGCTTCCAGACGCTGCTCCATCGACTGCATCACTTCGCTGACCAGGTACTTg                                                                                                                                                   <  4:185138/149‑1 (MQ=255)
cgccTTTTTTCGGGTCTTTCTTGATGATCTGCACGCGCTGACCCGCCACTTCCAGGCGCCAGTCCTCGGCCTTGGCCTGCGGATAGAAACGACGCAGGGCTTCCAGACGCTGCTCCATCGACTGCATCACTTCGCTGACCAGGTACTTg                                                                                                                                                   >  2:194232/1‑149 (MQ=255)
 gccTTTTTTCGGGTCTTTCTTGATGATCTGCACGCGCTGACCCGCCACTTCCAGGCGCCAGTCCTCGGCCTTGGCCTGCGGATAGAAACGACGCAGGGCTTCCAGACGCTGCTCCATCGACTGCATCACTTCGCTGACCAGGTACTTgg                                                                                                                                                  <  2:198723/149‑1 (MQ=255)
 gccTTTTTTCGGGTCTTTCTTGATGATCTGCACGCGCTGACCCGCCACTTCCAGGCGCCAGTCCTCGGCCTTGGCCTGCGGATAGAAACGACGAAGGGCTTCCAGACCCTGCTCCATCGACTGCATCACTTCGCTGACCAGGTACTTgg                                                                                                                                                  >  7:77207/1‑149 (MQ=255)
  ccTTTTTTCGGGTCTTTCTTGATGATCTGCACGCGCTGACCCGCCACTTCCAGGCGCCAGTCCTCGGCCTTGGCCTGCGGATAGAAACGACGCAGGGCTTCCAGACGCTGCTCCATCGACTGCATCACTTCGCTGACCAAGTACCTGGt                                                                                                                                                 <  5:27151/149‑1 (MQ=255)
     tttttCGGGTCTTTCTTGATGATCTGCACGCGCTGACCCGCCACTTCCAGGCGCCAGTCCTCGGCCTTGGCCTGCGGATAGAAACGACGCAGGGCTTCCAGACGCTGCTCCATCGACTGCATCACTTCGCTGACCAGGTACTTGGTCAg                                                                                                                                              >  1:86573/1‑149 (MQ=255)
      ttttCGGGTCTTTCTTGATGATCTGCACGCGCTGACCCGCCACTTCCAGGCGCCAGTCCTCGGCCTTGGCCTGCGGATAGAAACGACGCAGGGCTTCCAGACGCTGCTCCATCGACTGTATCACTTCGCTGACCAAGTACCTGGTCa                                                                                                                                               <  1:214154/147‑1 (MQ=255)
       tttCGGGTCTTTCTTGATGATCTGCACGCGCTGACCCGCCACTTCCAGGCGCCAGTCCTCGGCCTTGGCCTGCGGATAGAAACGACGCAGGGCTTCCAGACGCTGCTCCATCGACTGCATCACTTCGCTGACCAGGTACTTGGTCAgg                                                                                                                                             <  5:268543/148‑1 (MQ=255)
            ggTCTTTCTTGATGATCTGCACGCGCTGACCCGCCACTTCCAGGCGCCAGTCCTCGGCCTTGGCCTGCGGATAGAAACGACGCAGGGCTTCCAGACGCTGCTCCATCGACTGCATCACTTCGCTGACCAGGTACTTGGTCAGGTCCAtg                                                                                                                                       <  4:342105/149‑1 (MQ=255)
                  tcttGATGATCTGCACGCGCTGACCCGCCACTTCCAGGCGCCAGTCCTCGGCCTTGGCCTGCGGATAGAAACGACGCAGGGCTTCCAGACGCTGCTCCATCGACTGCATCACTTCGCTGACCAGGTACTTGGTCAGGTCCAtgttg                                                                                                                                    <  2:105241/146‑1 (MQ=255)
                      gatgatCTGCACGCGCTGACCCGCCACTTCCAGGCGCCAGTCCTCGGCCTTGGCCTGCGGATAGAAACGACGCAGGGCTTCCAGACGCTGCTCCATCGACTGCATCACTTCGCTGACCAGGTACTTGGTCAGGTCCATGTTGTCGCggg                                                                                                                             >  2:265416/1‑149 (MQ=255)
                      gatgatCTGCACGCGCTGACCCGCCACTTCCAGGCGCCAGTCCTCGGCCTTGGCCTGCGGATAGAAACGACGCAGGGCTTCCAGACGCTGCTCCATCGACTGCATCACTTCGCTGACCAGGTACTTGGTCAGGTCCATGTTGTCGCgg                                                                                                                              <  1:52355/148‑1 (MQ=255)
                         gatCTGCACGCGCTGACCCGCCACTTCCAGGCGCCAGTCCTCGGCCTTGGCCTGCGGATAGAAACGACGCAGGGCTTCCAGACGCTGCTCCATCGACTGCATCACTTCGCTGACCAGGTACTTGGTCAGGTCCATGTTGTCGCGGGCCa                                                                                                                          >  8:238570/1‑149 (MQ=255)
                             tGCACGCGCTGACCCGCCACTTCCAGGCGCCAGTCCTCGGCCTTGGCCTGCGGATAGAAACGACGCAGGGCTTCCAGACGCTGCTCCATCGACTGCATCACTTCGCTGACCAAGTACCTGGTCAgcgaa                                                                                                                                          <  3:178936/129‑5 (MQ=255)
                             tGCACGCGCTGACCCGCCACTTCCAGGCGCCAGTCCTCGGCCTTGGCCTGCGGATAGAAACGACGCAGGGCTTCCAGACGCTGCTCCATCGACTGCATCACTTCGCTGACCAAGTACCTGGTCAgcgaa                                                                                                                                          >  4:178936/1‑125 (MQ=255)
                               cACGCGCTGACCCGCCACTTCCAGGCGCCAGTCCTCGGCCTTGGCCTGCGGATAGAAACGACGCAGGGCTTCCAGACGCTGCTCCATCGACTGCATCACTTCGCTGACCAGGTACTTGGTCAGGTCCATGTTGTCGCGGGCCACGGc                                                                                                                      >  4:142054/1‑147 (MQ=255)
                               cACGCGCGGACCCGCCACTTCCAGGCGCCAGTCCTCGGCCTTGGCCTGCGGATAGAAACGACGCAGGGCTTCCAGACGCTGCTCCATCGACTGCATCACTTCGCTGACCAGGTACTTGGTCAGGTCCATGTTGTCGCGGGCCACGGc                                                                                                                      <  3:36856/147‑1 (MQ=255)
                                    gcTGACCCGCCACTTCCAGGCGCCAGTCCTCGGCGTTGGCCTGCGGATAGAAACGACGCAGGGCTTCCAGACGCTGCTCCATCGAGTGCATCACTTCGCTGACCAGGTACTTTGGCAGGTCCATGTTGTCGCGGGCCACGGCCAGCATc                                                                                                               <  1:340268/149‑1 (MQ=255)
                                            gCCACTTCCAGGCGCCAGTCCTCGGCCTTGGCCTGCGGATAGAAACGACGCAGGGCTTCCAGACGCTGCTCCATCGCCTGCATCACTTCGCTGACCAGGTACTTGGTCAGGTCCATGTTGTCGCGGGCCACGGCCAGCATCGGGCCGAt                                                                                                       >  3:233044/1‑149 (MQ=255)
                                               aCTTCCAGGCGCCAGTCCTCGGCCTTGGCCTGCGGATAGAAACGACGCAGGGCTTCCAGACGCTGCTCCATCGACTGCATCACTTCGCTGACCAGGTACTTGGTCAGGTCCATGTTGTCGCGGGCCACGGCCAGCATCGGGCCGATGtt                                                                                                    <  4:67557/149‑1 (MQ=255)
                                                cTTCCAGGCGCCAGTCCTCGGCCTTGGCCTGCGGATAGAAACGACGCAGGGCTTCCAGACGCTGCTCCATCGACTGCATCACTTCGCTGACCAGGTACTTGGTCAGGTCCATGTTGTCGCGGGCCACGGCCAGCATCGGGCCGATGTTg                                                                                                   <  8:17987/149‑1 (MQ=255)
                                                 ttCCAGGCGCCAGTCCTCGGCCTTGGCCTGCGGATAGAAACGACGCAGGGCTTCCAGACGCTGCTCCATCGCCTGCATCACTTCGCTGACCAGGTACTTGGTCAGGTCCATGTTGTCGCGGGCCACGGCCAGCATCGGGCCGATGTTGc                                                                                                  >  6:7921/1‑149 (MQ=255)
                                                   ccAGGCGCCAGTCCTCGGCCTTGGCCTGCGGATAGAAACGACGCAGGGCTTCCAGACGCTGCTCCATCGACTGCATCACTTCGCTGACCAGGTACTTGGTCAGGTCCATGTTGTCGCGGGCCACGGCCAGCATCGGGCCGATGTTGc                                                                                                  <  2:86573/147‑1 (MQ=255)
                                                    cAGGCGCCAGTCCTCGGCCTTGGCCTGCGGATAGAAACGACGCAGGGCTTCCAGACGCTGCTCCATCGCATGCATCACTTCGCTGACCAGGTACTTGGTCAGGTCCATGTTGTCGCGGGCCACGGCCAGCATCGGGCCGATGTTGccc                                                                                                <  7:238570/148‑1 (MQ=255)
                                                    cAGGCGCCAGTCCTCGGCCTTGGCCTGCGGATAGAAACGACGCAGGGCTTCCAGACGCTGCTCCATCGACTGCATCACTTCGCTGACCAGGTACTTGGTCAGGTCCATGTTGTCGCGGGCCACGGCCAGCATCGGGCCGATGTTGccc                                                                                                >  3:27096/1‑148 (MQ=255)
                                                    cAGGCGCCAGTCCTCGGCCTTGGCCTGCGGATAGAAACGACGCAGGGCTTCCAGACGCTGCTCCATCGACTGCATCACTTCGCTGACCAGGTACTTGGTCAGGTCCATGTTGTCGCGGGCCACGGCCAGCATCGGGCCGATGTTGccc                                                                                                <  4:27096/148‑1 (MQ=255)
                                                    cAGGCGCCAGTCCTCGGCCTTGGCCTGCGGATAGAAACGACGCAGGGCTTCCAGACGCTGCTCCATCGACTGCATCACTTCGCTGACCAGGTACTTGGTCAGGTCCATGTTGTCGCGGGCCACGGCCAGCATCGGGCCGATGTTGCCCa                                                                                               >  5:133171/1‑149 (MQ=255)
                                                     aGGCGCCAGTCCTCGGCCTTGGCCTGCGGATAGAAACGACGCAGGGCTTCCAGACGCTGCTCCATCGACTGCATCACTTCGCTGACCAGGTACTTGGTCAGGTCCATGTTGTCGCGGGCCACGGCCAGCATCGGGCCGATGTTGCCCAt                                                                                              >  1:211775/1‑149 (MQ=255)
                                                      ggCGCCAGTCCTCGGCCTTGGCCTGCGGATAGAAACGACGCAGGGCTTCCAGACGCTGCTCCATCGACTGCATCACTTCGCTGACCAGGTACTTGGTCAGGTCCATGTTGTCGCGGGCCACGGCCAGCATCGGGCCGATGTTGCCCATg                                                                                             <  2:211775/149‑1 (MQ=255)
                                                           cAGTCCTCGGCCTTGGCCTGCGGATAGAAACGACGCAGGGCTTCCAGACGCTGCTCCATCGACTGCATCACTTCGCTGACCAGGTACTTGGTCAGGTCCATGTTGTCGCGGGCCACGGCCAGCATCGGGCCGATGTTGCCCATGCGCAc                                                                                        <  2:314370/149‑1 (MQ=255)
                                                           cAGTCCTCGGCCTTGGCCTGCGGATAGAAACGACGCAGGGCTTCCAGACGCTGCTCCATCGACTGCATCACTTCGCTGACCAGGTACTTGGTCAGGTCCATGTTGTCGCGGGCCACGGCCAGCATCGGGCCGATGTTGCCCATGCGCAc                                                                                        <  3:142054/149‑1 (MQ=255)
                                                           cAGTCCTCGGCCTTGGCCTGCGGATAGAAACGACGCAGGGCTTCCAGACGCTGCTCCATCGACTGCATCACTTCGCTGACCAGGTACTTGGTCAGGTCCATGTTGTCGCGGGCCACGGCCAGCATCGGGCCGATGTTGCCCATGCGCAc                                                                                        >  3:85639/1‑149 (MQ=255)
                                                                     ccTTGGCCTGCGGATAGAAACGACGCAGGGCTTCCAGACGCTGCTCCATCGACTGCATCACTTCGCTGACCAGGTACTTGGTCAGGTCCATGTTGTCGCGGGCCACGGCCAGCATCGGGCCGATGTTGCCCATGCGCACCGACAGCGGc                                                                              <  1:194232/149‑1 (MQ=255)
                                                                         ggCCTGCGGATAGAAACGACGCAGGGCTTCCAGACGCTGCTCCATCGACTGCATCACTTCGCTGACCAGGTACTTGGTCAGGTCCATGTTGTCGCGGGCCACGGCCAGCATCGGGCCGATGTTGCCCATGCGCACCGACAGCGGCAg                                                                            <  2:108252/147‑1 (MQ=255)
                                                                         ggCCTGCGGATAGAAACGACGCAGGGCTTCCAGACGCTGCTCCATCGACTGCATCACTTCGCTGACCAGGTACTTGGTCAGGTCCATGTTGTCGCGGGCCACGGCCAGCATCGGGCCGATGTTGCCCATGCGCACCGACAGCGGCAg                                                                            <  1:265416/147‑1 (MQ=255)
                                                                                ggATAGACACGTCGCAGGGCTTCCAGACGCTGCTCGATCGACTGCATCACTTCGCTGACCAGGTACTTGGTCAGGTCCATGTTGTCGCGGGCCACGGCCAGCATCGGGCCGATGTTGCCCATGCGCACCGACAGCGGCAGGTCCATCAg                                                                   <  8:77207/149‑1 (MQ=255)
                                                                                      aaaCGACGCAGGGCTTCCAGACGCTGCTCCATCGACTGCATCACTTCGCTGACCAGGTACTTGGTCAGGTCCATGTTGTCGCGGGCCACGGCCAGCATCGGGCCGATGTTGCCCATGCGCACCGACAGCGGCAGGt                                                                          >  3:9004/1‑136 (MQ=255)
                                                                                      aaaCGACGCAGGGCTTCCAGACGCTGCTCCATCGACTGCATCACTTCGCTGACCAGGTACTTGGTCAGGTCCATGTTGTCGCGGGCCACGGCCAGCATCGGGCCGATGTTGCCCATGCGCACCGACAGCGGCAGGt                                                                          <  4:9004/136‑1 (MQ=255)
                                                                                      aaaCGACGCAGGGCTTCCAGACGCTGCTCCATCGACTGCATCACTTCGCTGACCAGGTACTTGGTCAGGTCCATGTTGTCGCGGGCCACGGCCAGCATCGGGCCGATGTTGCCCATGCGCACCGACAGCGGCAGGTCCATCAGCGAGcc                                                             >  4:191198/1‑149 (MQ=255)
                                                                                          gacgCAGGCCTTCCAGACGCTGCTGCATCGACTGCATCACTTCGCTGACCAGGTACTTGGTCAGGTCCAAGTTGTCGCGGGCCACGGCCAGCATCGGGCCGATGTTGCCCATGCGCAGCGCCAGCGGCAGGTCCATCAGCGACGCGTGc                                                         >  6:184864/1‑149 (MQ=255)
                                                                                               aGGGCTTCCAGACGCTGCTCCATCGACTGCATCACTTCGCTGACCAGGTACTTGGTCAGGTCCATGTTGTCGCGGGCCACGGCCAGCATCGGGCCGATGTTGCCCATGCGCACCGACAGCGGCAGGTCCATCAGCGAGCCGTGCTTGAg                                                    >  4:107613/1‑149 (MQ=255)
                                                                                                 ggCTTCCAGACGCTGCTCCATCGACTGCATCACTTCGCTGACCAGGTACTTGGTCAGGTCCATGTTGTCGCGGGCCACGGCCAGCATCGGGCCGATGTTGCCCATGCGCACCGa                                                                                     >  4:298793/1‑114 (MQ=255)
                                                                                                 ggCTTCCAGACGCTGCTCCATCGACTGCATCACTTCGCTGACCAGGTACTTGGTCAGGTCCATGTTGTCGCGGGCCACGGCCAGCATCGGGCCGATGTTGCCCATGCGCACCGa                                                                                     <  3:298793/114‑1 (MQ=255)
                                                                                                    ttCCAGACGCTGCTCCATCGACTGCATCACTTCGCTGACCAGGTACTTGGTCAGGTCCATGTTGTCGCGGGCCACGGCCAGCATCGGGCCGATGTTGCCCATGCGCACCGACAGCGGCAGGTCCATCAGCGAGCCGTGCTTGAGGAACt                                               >  4:356308/1‑149 (MQ=255)
                                                                                                      ccAGACGCTGCTCCATCGACTGCATCACTTCGCTGACCAGGTACTTGGTCAGGTCCATGTTGTCGCGGGCCACGGCCAGCATCGGGCCGATGTTGCCCATGCGCACCGACAGCGGCAGGTCCATCAGCGAGCCGTGCTTGAGGAACTTg                                             >  1:26749/1‑149 (MQ=255)
                                                                                                       cAGACGCTGCTCCATCGACTGCATCACTTCGCTGACCAGGTACTTGGTCAGGTCCATGTTGTCGCGGGCCACGGCCAGCATCGGGCCGATGTTGCCCATGCGCACCGACAGCGGCAGGTCCATCAGCGAGCCGTGCTTGAGGAACTTgg                                            <  4:133295/149‑1 (MQ=255)
                                                                                                         gACGCTGCTCCATCGACTGCATCACTTCGCTGACCAGGTACTTGGTCAGGTCCATGTTGTCGCGGGCCACGGCCAGCATCGGGCCGATGTTGCCCATGCGCACCGACAGCGGCAGGTCCATCAGCGAGCCGTGCTTGAGGAACTTGGTa                                          <  1:178126/149‑1 (MQ=255)
                                                                                                         gACGCTGCTCCATCGACTGCATCACTTCGCTGACCAGGTACTTGGTCAGGTCCATGTTGTCGCGGGCCACGGCCAGCATCGGGCCGATGTTGCCCATGCGCACCGACAGCGGCAGGTCCATCAGCGAGCCGTGCTTGAGGAACTTGGTa                                          <  2:3066/149‑1 (MQ=255)
                                                                                                          aCGCTGCTCCATCGACTGCATCACTTCGCTGACCAGGTACTTGGTCAGGTCCATGTTGTCGCGGGCCACGGCCAGCATCGGGCCGATGTTGCCCATGCGCACCGACAGCGGCAGGTCCATCAGCGAGCCGTGCTTGAGGAACTTGGTAg                                         <  4:85639/149‑1 (MQ=255)
                                                                                                            gctgctCCATCGACTGCATCACTTCGCTGACCAGGTACTTGGTCAGGTCCATGTTGTCGCGGGCCACGGCCAGCATCGGGCCGATGTTGCCCATGCGCACCGACAGCGGCAGGTCCATCAGCGAGCCGTGCTTGAGGAACTTGGTAGt                                        >  7:54327/1‑148 (MQ=255)
                                                                                                              tgctCCATCGACTGCATCACTTCGCTGACCAGGTACTTGGTCAGGTCCATGTTGTCGCGGGCCACGGCCAGCATCGGGCCGATGTTGCCCATGCGCACAGACAGCGGCAGGTCCATCAGCGAGCCGTGCTTGAGGAACTTGGTAGTGa                                      >  2:260063/1‑148 (MQ=255)
                                                                                                                   cATCGACTGCATCACTTCGCTGACCAGGTACTTGGTCAGGTCCATGTTGTCGCGGGCCACGGCCAGCATCGGGCCGATGTTGCCCATGCGCACCGACAGCGGCAGGTCCATCAGCGAGCCGTGCTTGAGGAACTTGGTAGTGAAACCgg                                >  1:171654/1‑149 (MQ=255)
                                                                                                                   cATCGACTGCATCACTTCGCTGACCAGGTACTTGGTCAGGTCCATGTTGTCGCGGGCCACGGCCAGCATCGGGCCGATGTTGCCCATGCGCACCGACAGCGGCAGGTCCATCAGCGAGCCGTGCTTGAGGAACTTGGTAGTGAAACCgg                                <  3:284009/149‑1 (MQ=255)
                                                                                                                   cATCGACTGCATCACTTCGCTGACCAGGTACTTGGTCAGGTCCATGTTGTCGCGGGCCACGGCCAGCATCGGGCCGATGTTGCCCATGCGCACCGACAGCGGCAGGTCCATCAGCGAGCCGTGCTTGAGGAACTTGGTAGTGAAACCg                                 <  6:252703/148‑1 (MQ=255)
                                                                                                                          tGCATCACTTCGCTGACCAGGTACTTGGTCAGGTCCATGTTGTCGCGGGCCACGGCCAGCATCGGGCCGATGTTGCCCATGCGCACCGACAGCGGCAGGTCCATCAGCGAGCCGTGCTTGAGGAACTTGGTAGTGAAACCGGCATACg                          >  7:260705/1‑148 (MQ=255)
                                                                                                                            cATCACTTCGCTGACCAGGTACTTGGTCAGGTCCATGTTGTCGCGGGCCACGGCCAGCATCGGGCCGATGTTGCCCATGCGCACCGACAGCGGCAGGTCCATCAGCGAGCCGTGCTTGAGGAACTTGGTAGTGAAACCGGCATACGGAc                       <  8:222832/149‑1 (MQ=255)
                                                                                                                             aTCACTTCGCTGACCAGGTACTTGGTCAGGTCCATGTTGTCGCGGGCCACGGCCAGCATCGGGCCGATGTTGCCCATGCGCACCGACAGCGGCAGGTCCATCAGCGAGCCGTGCTTGAGGAACTTGGTAGTGAAACCGGCATACGGAcc                      >  5:352935/1‑149 (MQ=255)
                                                                                                                                aCTTCGCTGACCAGGTACTTGGTCAGGTCCATGTTGTCGCGGGCCACGGCCAGCATCGGGCCGATGTTGCCCATGCGCACCGACAGCGGCAGGTCCATCAGCGAGCCGTGCTTGAGGAACTTGGTAGTGAAACCGGCATACGGACCGaa                   <  3:107613/149‑1 (MQ=255)
                                                                                                                                 cTTCGCTGACCAGGTACTTGGTCAGGTCCATGTTGTCGCGGGCCACGGCCAGCATCGGGCCGATGTTGCCCATGCGCACCGACAGCGGCAGGTCCATCAGCGAGCCGTGCTTGAGGAACTTGGTAGTGAAACCGGCATACGGACCGaa                   >  3:130169/1‑148 (MQ=255)
                                                                                                                                 cTTCGCTGACCAGGTACTTGGTCAGGTCCATGTTGTCGCGGGCCACGGCCAGCATCGGGCCGATGTTGCCCATGCGCACCGACAGCGGCAGGTCCATCAGCGAGCCGTGCTTGAGGAACTTGGTAGTGAAACCGGCATACGGACCGaa                   >  1:227317/1‑148 (MQ=255)
                                                                                                                                    cGCTGACCAGGTACTTGGTCAGGTCCATGTTGTCGCGGGCCACGGCCAGCATCGGGCCGATGTTGCCCATGCGCACCGACAGCGGCAGGTCCATCAGCGAGCCGTGCTTGAGGAACTTGGTAGTGAAACCGGCATACGGACCGAAcag                <  4:130169/148‑1 (MQ=255)
                                                                                                                                     ccTGACCAAGTACCTGGTCAGGTCCATGTTGTCGCGGGCCACGGCCAGCATCGGGCCGATGTTGCCCATGCGCACCGACAGCGGCAGGTCCATCAGCGAGCCGTGCTTGAGGAACTTGGTAGTGAAACCGGCATACGGACCGAAcag                >  3:359799/2‑147 (MQ=255)
                                                                                                                                      cTGACCAGGTACTTGGTCAGGTCCATGTTGTCGCGGGCCACGGCCAGCATCGGGCCGATGTTGCCCATGCGCACCGACAGCGGCAGGTCCATCAGCGAGCCGTGCTTGAGGAACTTGGTAGTGAAACCGGCATACGGACCGAAcagcag             <  8:260705/149‑1 (MQ=255)
                                                                                                                                          ccAGGTACTTGGTCAGGTCCATGTTGTCGCGGGCCACGGCCAGCATCGGGCCGATGTTGCCCATGCGCACCGACAGCGGCAGGTCCATCAGCGAGCCGTGCTTGAGGAACTTGGTAGTGAAACCGGCATACGGACCGAACAGCAGCGaa         <  6:190484/149‑1 (MQ=255)
                                                                                                                                          ccAGGTACTTGGTCAGGTCCATGTTGTCGCGGGCCACGGCCAGCATCGGGCCGATGTTGCCCATGCGCACCGACAGCGGCAGGTCCATCAGCGAGCCGTGCTTGAGGAACTTGGTAGTGAAACCGGCATACGGACCGAACAGCAGCGaa         <  2:171654/149‑1 (MQ=255)
                                                                                                                                              gTACTTGGTCAGGTCCATGTTGTCGCGGGCCACGGCCAGCATCGGGCCGATGTTGCCCATGCGCACCGACAGCGGCAGGTCCATCAGCGAGCCGTGCTTGAGGAACTTGGTAGTGAAAGCGGCGTACGGACCGCACAGCAGCGCAGTCt     >  4:228493/1‑149 (MQ=255)
                                                                                                                                               tACTTGGTCAGGTCCATGTTGTCGCGGGCCACGGCCAGCATCGGGCCGATGTTGCCCATGCGCACCGACAGCGGCAGGTCAATCAGCGAGCCGTGCTTGAGGAACTTGGTACTGAAACCCGCATACGGACCGAACAGCAGCGAAGTCtt    >  4:285877/1‑149 (MQ=255)
                                                                                                                                                 cTTTGTCAGGGCCATGTTGTCGCGGGCCACGGCCAGCATCGGGCCGATGTTGCCCATGCGCACCGACAGCGGCAGGTCCATCAGCGAGCCGTGCTTGAGGAACTTGGTAGTGAAACCGGCATACGGACCGAACAGCAGCGAAGTCTTGc  <  4:233044/149‑1 (MQ=255)
                                                                                                                                                 cTTGGTCAGTTCCATGTTGTCGCGGGCCACGGCCAGCATCGGGCCGATGTTGCCCATGCGCACCGACAGCGGCAGGTCCATCAGCGAGCCGTGCTTGAGGAACTTGGTAGTGAAACCGGCATACGGACCGAACAGCAGCGAAGTCTTg   <  1:129725/148‑1 (MQ=255)
                                                                                                                                                 cTTGGTCAGGTCCATGTTGTCGCGGGCCACGGCCAGCATCGGGCCGATGTTGCCCATGCGCACCGACAGCTGCAGGTCCATCAGCGAGCCGTGCTTGAGGAACTTGGTAGTGAAACCGGCATATGGACCGAACAGCAGCGAAGTCTTg   <  8:54327/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
CGCCTTTTTTCGGGTCTTTCTTGATGATCTGCACGCGCTGACCCGCCACTTCCAGGCGCCAGTCCTCGGCCTTGGCCTGCGGATAGAAACGACGCAGGGCTTCCAGACGCTGCTCCATCGACTGCATCACTTCGCTGACCAGGTACTTGGTCAGGTCCATGTTGTCGCGGGCCACGGCCAGCATCGGGCCGATGTTGCCCATGCGCACCGACAGCGGCAGGTCCATCAGCGAGCCGTGCTTGAGGAACTTGGTAGTGAAACCGGCATACGGACCGAACAGCAGCGAAGTCTTGC  >  NC_002947/868369‑868662

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑