Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	898,295	T→G	5.1%	F60C (TTC→TGC)	PP_0781 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	898,295	0	T	G	5.1%	291.9 / 3.8	92	F60C (TTC→TGC)	PP_0781	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (43/44);  new base G (3/2);  total (46/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCTCGTGCCCTCCTGGCACGAGATCGAGTCACGTGTCATCGCCATGGTGGGCTACCCAAGCGGTGGCCAGTTCCGCACCAGCCTCAATGAAGAAGTCGCCTCGCTGGGCCTCAACCTGCGCCGGATCGACGCTGTGCGCTCAGCCTTCTACCAGGCTGAATGGCAGACGGCGCACATGCTACGCCAGCGCTTCGTCGACCTCGACATCAACAGCATCGTCGACGAACTGCTGAGGGTCGTGCAGCAGATGGCGATGATCGTCGTCGGCAGCACCGTTACCGGTGGCCTGCTTGGC  >  NC_002947/898149‑898443                                                                                                                                                   |                                                                                                                                                     ccTCGTGCCCTCCTGGCACGAGATCGAGTCACGTGTCATCGCCATGGTGGGCTACCCAAGCGGTGGCCAGTTCCGCACCAGCCTCAATGAAGAAGTCGCCTCGCTGGGCCTCAACCTGCGCCGGATCGACGCTGTGCGCTCAGCCTTc                                                                                                                                                     <  8:153695/148‑1 (MQ=255)  cTCGTGCCCTCCTGGCACGAGATCGAGTCACGTGTCATCGCCATGGTGGGCTACCCAAGCGGTGGCCAGTTCCGCACCAGCCTCAATGAAGAAGTCGCCTCGCTGGGCCTCAACCTGCGCCGGATCGACGCTGTGCGCTCAGCCTTCTa                                                                                                                                                   >  2:258012/1‑149 (MQ=255)     gTGCCCTCCTGGCACGAGATCGAGTCACGTGTCATCGCCATGGTGGGCTACCCAAGCGGTGGCCAGTTCCGCACCAGCCTCAATGAAGAAGTCGCCTCGCTGGGCCTCAACCTGCGCCGGATCGACGCTGTGCGCTCAGCCTTCTAc                                                                                                                                                  <  7:91256/147‑1 (MQ=255)     gTGCCCTCCTGGCACGAGATCGAGTCACGTGTCATCGCCATGGTGGGCTACCCAAGCGGTGGCAAGGTCCGCACCCGCCTCACTGAAGCATTCGCCTCGCTGGGCCTCAACCTGCGCCGGATCGACGCTGTGCGCTCAGCCTTCTACCa                                                                                                                                                <  7:328453/149‑1 (MQ=14)          ctcctGGCACGAGATCGAGTCACGTGTCATCGCCATGGTGGGCTACCCAAGCGGTGGCCAGTTCCGCACCAGCCTCAATGAAGAAGTCGCCTCGCTGGGCCTCAACCTGCGCCGGATCGCCGCTGTGCTCTCAGCCTGCTACCAGGCt                                                                                                                                            >  6:89587/1‑148 (MQ=255)             ctGGCACGAGATCGAGTCACGTGTCATCGCCATGGTGGGCTACCCAAGCGGTGGCCAGTTCCGCACCAGCCTCAATGAAGAAGTCGCCTCGCTGGGCCTCAACCTGCGCCGGATCGACGCTGTGCGCTCAGCCTTCTACCAGGCTGAAt                                                                                                                                        <  6:134829/149‑1 (MQ=255)             ctGGCACGAGATCGAGTCACGTGTCATCGCCATGGTGGGCTACCCAAGCGGTGGCCAGTTCCGCACCAGCCTCAATGAAGAAGTCGCCTCGCTGGGCCTCAACCTGCGCCGGATCGACGCTGTGCGCTCAGCCTTCTACCAGGCTGAAt                                                                                                                                        <  5:368712/149‑1 (MQ=255)             ctGGCACGAGATCGAGTCACGTGTCATCGCCATGGTGGGCTACCCAAGCGGTGGCCAGTTCCGCACCAGCCTCAATGAAGAAGTCGCCTCGCTGGGCCTCAACCTGCGCCGGATCGACGCTGTGCGCTCAGCCTTCTACCAGGCTGAAt                                                                                                                                        <  5:165665/149‑1 (MQ=255)             ctGGCACGAGATCGAGTCACGTGTCATCGCCATGGTGGGCTACCCAAGCGGTGGCCAGTTCCGCACCAGCCTCAATGAAGAAGTCGCCTCGCTGGGCCTCAACCTGCGCCGGATCGACGCTGTGCGCTCAGCCTGGTAGAAGGCTGa                                                                                                                                          <  6:46617/147‑1 (MQ=255)             ctGGCACGAGATCGAGTCACGTGTCATCGACATGGTGGGCTACCCAAGCGGTGGCCAGTTCCGCACCAGCCTCAATGAAGAAGTCGCCTCGCTGGGCCTCAACCTGCGCCGGATCGACGCTGTGCGCTCAGCCTTCTACCAGGCTGaa                                                                                                                                         <  4:275420/148‑1 (MQ=255)             agggCACGAGATCGAGTCACGTGTCATCGCCATGGTGGGCTACCCAAGCGGTGGCCAGTTCCGCACCAGCCTCAATGAAGAAGTCGCCTCGCTGGGCCTCAACCTGCGCCGGATCGACGCTGTGCGCTCAGCCTTCTACCAGGCTGAAt                                                                                                                                        <  8:111574/147‑1 (MQ=255)                gCACGAGATCGAGTCACGTGTCATCGCCATGGTGGGCTACCCAAGCGGTGGCCAGTTCCGCACCAGCCTCTATGAAGAAGTCGCCTCGCTGGGCCTCAACCTGCGCCGGATCGACGCTGTGCGCTCAGCCTTCTACCAGGCTGAATGGc                                                                                                                                     <  6:310806/149‑1 (MQ=255)                    gagaTCGAGTCACGTGTCATCGCCATGGTGGGCTACCCAAGCGGTGGCCAGTTCCGCACCAGCCTCAATGAAGAAGTCGCCTCGCTGGGCCTCAACCTGCGCCGGATCGACGCTGTGCGCTCAGCCTTCTACCAGGCTGAATGGCAGAc                                                                                                                                 >  4:212287/1‑149 (MQ=255)                      gaTCGAGTCACGTGTCATCGCCATGGTGGGCTACCCAAGCGGTGGCCAGTTCCGCACCAGCCTCAATGAAGAAGTCGCCTCGCTGGGCCTCAACCTGCGCCGGATCGACGCTGTGCGCTCAGCCTTCTACCAGGCTGAATGGCAGACgg                                                                                                                               >  7:11582/1‑149 (MQ=255)                      gaTCGAGTCACGTGTCATCGCCATGGTGGGCTACCCAAGCGGTGGCCAGTTCCGCACCAGCCTCAATGAAGAAGTCGCCTCGCTGGGCCTCAACCTGCGCCGGATCGACGCTGTGCGCTCAGCCTTCTACCAGGCTGAATGGCAGACgg                                                                                                                               >  3:253677/1‑149 (MQ=255)                           aGTCACGTGTCATCGCCATGGTGGGCTACCCAAGCGGTGGCCAGTTCCGCACCAGCCTCAATGAAGAAGTCGCCTCGCTGGGCCTCAACCTGCGCCGGATCGACGCTGTGCGCTCAGCCTTCTACCAGGCTGAATGGCAGACGGCGcac                                                                                                                          <  1:125897/149‑1 (MQ=255)                               aCGTGTCATCGCCATGGTGGGCTACCCAAGCGGTGGCCAGTTCCGCACCAGCCTCAATGAAGAAGTCGCCTCGCTGGGCCTCAACCTGCGCCGGATCGACGCTGTGCGCTCAGCCtt                                                                                                                                                      <  4:35412/117‑1 (MQ=255)                               aCGTGTCATCGCCATGGTGGGCTACCCAAGCGGTGGCCAGTTCCGCACCAGCCTCAATGAAGAAGTCGCCTCGCTGGGCCTCAACCTGCGCCGGATCGACGCTGTGCGCTCAGCCtt                                                                                                                                                      >  3:35412/1‑117 (MQ=255)                                    tCATCGCCATGGTGGGCTACCCAAGCGGTGGCCAGTTCCGCACCAGCCTCAATGAAGAAGTCGCCTCGCTGGGCCTCAACCTGCGCCGGATCGACGCTGTGCGCTCAGCCTTCTACCAGGCTGAATGGCAGACGGCGCACATGCTACGc                                                                                                                 <  2:241847/149‑1 (MQ=255)                                     cATCGCCATGGTGGGCTACCCAAGCGGTGGCCAGTTCCGCACCAGCCTCAATGAAGAAGTCGCCTCGCTGGGCCTCAACCTGCGCCGGATCGACGCTGTGCGCTCAGCCTTCTACCAGGCTGAATGGCAGACGGCGCACATGCTACGc                                                                                                                 >  1:212245/1‑148 (MQ=255)                                      aTCGCCATGGTGGGCTACCCAAGCGGTGGCCAGTTCCGCACCAGCCTCAATGAAGAAGTCGCCTCGCTGGGCCTCAACCTGCGCCGGATCGACGCTGTGCGCTCAGCCTTCTACCAGGCTGAATGGCAGACGGCGCACATGCTACGc                                                                                                                 >  2:170630/1‑147 (MQ=255)                                           cATGGTGGGCTACCCAAGCGGTGGCCAGTTCCGCACCAGCCTCAATGAAGAAGTCGCCTCGCTGGGCCTCAACCTGCGCCGGATCGACGCTGTGCGCTCAGCCTTCTACCAGGCTGAATGGCAGACGGCGCACATGCTACGCCAGCGCt                                                                                                          >  7:275118/1‑149 (MQ=255)                                             tggtggGCTACCCAAGCGGTGGCCAGTTCCGCACCAGCCTCAATGAAGAAGTCGCCTCGCTGGGCCTCAACCTGCGCGGGATCGACGCTGTGCGCTCAGCCTTCTACCAGGCTGAATGGCAGACGGCGCACATGCTACGCCAGCGCTtc                                                                                                        <  6:100185/149‑1 (MQ=255)                                               gtggGCTACCCAAGCGGTGGCCAGTTCCGCACCAGCCTCAATGAAGAAGTCGCCTCGCTGGGCCTCAACCTGCGCCGGATCGACGCTGTGCGCTCAGCCTTCTACCAGGCTGAATGGCAGACGGCGCACATGCTACGCCAGCGCTtcgt                                                                                                      >  3:262247/1‑149 (MQ=255)                                               gtggGCTACCCAAGCGGTGGCCAGTTCCGCACCAGCCTCAATGAAGAAGTCGCCTCGCTGGGCCTCAACCTGCGCCGGATCGACGCTGTGCGCTCAGCCTTCTACCAGGCTGAATGGCAGACGGCGCACATGCTACGCCAGCGCTtcgt                                                                                                      <  4:127929/149‑1 (MQ=255)                                                 gggCTACCCAAGCGGTGGCCAGTTCCGCACCAGCCTCAATGAAGAAGTCGCCTCGCTGGGCCTCAACCTGCGCCGGATCGACGCTGTGCGCTCAGCCTTCTACCAGGCTGAATGGCAGACGGCGCACATGCTACGCCAGCGCTtcgtc                                                                                                     >  2:252186/1‑148 (MQ=255)                                                  ggCTACCCAAGCGGTGGCCAGTTCCGCACCAGCCTCAATGAAGAAGTCGCCTCGCTGGGCCTCAACCTGCGCCGGATCGACGCTGTGCGCTCAGCCTTCTACCAGGCTGAATGGCAGACGGCGCACATGCTACGCCAGCGCTTCGTCGa                                                                                                   >  8:175904/1‑149 (MQ=255)                                                  ggCTACCCAAGCGGTGGCCAGTTCCGCACCAGCCTCAATGAAGAAGTCGCCTCGCTGGGCCTCAACCTGCGCCGGATCGACGCTGTGCGCTCAGCCTTCTACCAGGCTGAATGGCAGACGGCGCACATGCTACGCCAGCGCTTCGTCGa                                                                                                   <  1:258012/149‑1 (MQ=255)                                                    cTACCCAAGCGGTGGCCAGTTCCGCACCAGCCTCAATGAAGAAGTCGCCTCGCTGGGCCTCAACCTGCGCCGGATCGACGCTGTGCGCTCAGCCTGGTAGAAGGCTGagc                                                                                                                                        >  4:286360/1‑108 (MQ=17)                                                     tACCCAAGCGGTGGCCAGTTCCGCACCAGCCTCAATGAAGAAGTCGCCTCGCTGGGCCTCAACCTGCGCCGGATCGACGCTGTGCGCTCAGCCTTCTACCAGGCTGAATGGCAGACGGCGCACATGCTACGCCAGCGCTTCGTCGACCt                                                                                                >  6:204455/1‑149 (MQ=255)                                                        ccAAGCGGTGGCCAGTTCCGCACCAGCCTCAATGAAGAAGTCGCCTCGCTGGGCCTCAACCTGCGCCGGATCGACGCTGTGCGCTCAGCCTTCTACCAGGCTGAATGGCAGACGGCGCACATGCTACGCCAGCGCTTCGTCGACCTCGa                                                                                             >  1:371848/1‑149 (MQ=255)                                                         cAAGCGGTGGCCAGTTCCGCACCAGCCTCAATGAAGAAGTCGCCTCGCTGGGCCTCAACCTGCGCCGGATCGACGCTGTGCGCTCAGCCTTCTACCAGGCTGAATGGCAGACGGCGCACATGCTACGCCAGCGCTTCGTCGACCTCGAc                                                                                            <  3:133557/149‑1 (MQ=255)                                                           aGCGGTGGCCAGTTCCGCACCAGCCTCAATGAAGAAGTCGCCTCGCTGGGCCTCAACCTGCGCCGGATCGACGCCGTGCGCTCAGCCTTCTACCAGGCTGAATGGCAGACGGCGCACATGCTACGCCAGCGCTTCGTCGACCTCGACAt                                                                                          >  5:1292/1‑149 (MQ=255)                                                            gCGGTGGCCAGTTCCGCACCAGCCTCAATGAAGAAGTCGCCTCGCTGGGCCTCAACCTGCGCCGGATCGACGCTGTGCGCTCAGCCTTCTACCAGGCTGAATGGCAGACGGCGCACATGCTACGCCAGCGCTTCGTCGACCTCGACATc                                                                                         <  4:253677/149‑1 (MQ=255)                                                                tGGCCAGTTCCGCACCAGCCTCAATGAAGAAGTCGCCTCGCTGGGCCTCAACCTGCGCCGGATCGACGCTGTGCGCTCAGCCTTCTACCAGGCTGAATGGCAGACGGCGCACATGCTACGCCAGCGCTTCGTCGACCTCGACATCAACa                                                                                     <  6:346867/149‑1 (MQ=255)                                                                  gCCAGTTCCGCACCAGCCTCAATGAAGAAGTCGCCTCGCTGGGCCTCAACCTGCGCCGGATCGACGCTGTGCGCTCAGCCTTCTACAAGGCTGAATGGCAGACGGCGAACATGCTACGCCAGCGCTTCGACGACCTCGACATCAACAg                                                                                    >  4:312171/1‑148 (MQ=255)                                                                   ccAGTTCCGCACCAGCCTCAATGAAGAAGTCGCCTCGCTGGGCCTCAACCTGCGCCGGATCGACGCTGTGCGCTCAGCCTTCTACCAGGCTGAATGGCAGACGGCGCACATGCTACGCCAGCGCTTCGTCGACCTCGACATCAACAGCa                                                                                  >  1:162924/1‑149 (MQ=255)                                                                    cAGTTCCGCACCAGCCTCAATGAAGAAGTCGCCTCGCTGGGCCTCAACCTGCGCCGGATCGACGCTGTGCGCTCAGCCTTCTACCAGGCTGAATGGCAGACGGCGCACATGCTACGCCAGCGCTTCGTCGACCTCGACATCAACAGCAt                                                                                 >  4:297594/1‑149 (MQ=255)                                                                     aGTTCCGCACCAGCCTCAATGAAGAAGTCGCCTCGCTGGGCCTCAACCTGCGCCGGATCGACGCTGTGCGCTCAGCCTTCTACCAGGCTGAATGGCAGACGGCGCACATGCTACGCCAGCGCTTCGTCGACCTCGACATCAACAGCAtc                                                                                <  2:303324/149‑1 (MQ=255)                                                                          cGCACCAGCCTCAATGAAGAAGTCGCCTCGCTGGGCCTCAACCTGCGCCGGATCGACGCTGTGCGCTCAGCCTTCTACCAGGCTGAATGGCAGACGGCGCACATGCTACGCCAGCGCTTCGTCGACCTCGACATCAACAGCAtcgtcg                                                                            <  2:212245/148‑1 (MQ=255)                                                                          cGCACCAGCCTCAATGAAGAAGTCGCCTCGCTGGGCCTCAACCTGCGCCGGATCGACGCTGTGCGCTCAGCCTTCTACCAGGCTGAATGGCAGACGGCGCACATGCTACGCCAGCGCTTCGTCGACCTCGACATCAACAGCATCGTcga                                                                           <  4:262247/149‑1 (MQ=255)                                                                           gCACCAGCCTCAATGAAGAAGTCGCCTCGCTGGGCCTCAACCTGCGCCGGATCGACGCTGTGCGCTCAGCCTTCTACCAGGCTGAATGGCAGACGGCGCACATGCTACGCCAGCGCTTCGTCGACCTCGACATCAACAGCATCGTcgac                                                                          <  6:53555/149‑1 (MQ=255)                                                                           gCACCAGCCTCAATGAAGAAGTCGCCTCGCTGGGCCTCAACCTGCGCCGGATCGACGCTGTGCGCTCAGCCTTCTACCAGGCTGAATGGCAGACGGCGCACATGCTACGCCAGCGCTTCGTCGACCTCGACATCAACAGCATCGTcgac                                                                          <  2:162924/149‑1 (MQ=255)                                                                             aCCAGCCTCAATGAAGAAGTCGCCTCGCTGGGCCTCAACCTGCGCCGGATCGACGCTGTGCGCTCAGCCTTCTACCAGGCTGAATGGCAGACGGCGCACATGCTACGCCAGCGCTTCGTCGACCTCGACATCAACAGCATCGTcgacg                                                                         >  6:12394/1‑148 (MQ=255)                                                                                 gCCTCAATGAAGAAGTCGCCTCGCTGGGCCTCAACCTGCGCCGGATCGACGCTGTGCGCTCAGCCTTCTACCAGGCTGAATGGCAGACGGCGCACATGCTACGCCAGCGCTTCGTCGCCCTCGACATCAACAGCATCGTCGACGAActg                                                                    >  2:66523/1‑149 (MQ=255)                                                                                  ccTCAATGAAGAAGTCGCCTCGCTGGGCCTCAACCTGCGCCGGATCGACGCTGTGCGCTCAGCCTTCTACCAGGCTGAATGGCAGACGGCGCACATGCTACGCCAGCGCTTCGTCGACCTCGACATCAACAGCATCGTCGACGAAc                                                                      >  7:68615/1‑146 (MQ=255)                                                                                  ccTCAATGAAGAAGTCGCCTCGCTGGGCCTCAACCTGCGCCGGATCGACGCTGTGCGCTCAGCCTTCTACCAGGCTGAATGGCAGACGGCGCACATGCTACGCCAGCGCTTCGTCGACCTCGACATCAACAGCATCGTCGACGAAc                                                                      <  8:68615/146‑1 (MQ=255)                                                                                    tCAATGAAGAAGTCGCCTCGCTGGGCCTCAACCTGCGCCGGATCGACGCTGTGCGCTCAGCCTTCTACCAGGCTGAATGGCAGACGGCGCACATGCTACGCCAGCGCTTCGTCGACCTCGACATCAACAGCATCGTCGACGAActgct                                                                  >  7:163107/1‑148 (MQ=255)                                                                                     cAATGAAGAAGTCGCCTCGCTGGGCCTCAACCTGCGCCGGATCGACGCTGTGCGCTCAGCCTTCTACCAGGCTGAATGGCAGACGGCGCACATGCTACGCCAGCGCTTCGTCGACCTCGACATCAACAGCATCGTCGACGAACTGCTGa                                                                <  7:175904/149‑1 (MQ=255)                                                                                      aaTGAAGAAGTTGCCTCGCTGGGCCTCAACCTGCGCCGGATCGACGCTGTGCGCTCAGCCTTCTACCAGGCTGAATGGCAGACGGCGCACATGCTACGCCAGCGCTTCGTCGACCTCGACATCAACAGCATCGTCGACGAGCTGCTGAg                                                               >  2:206399/1‑149 (MQ=255)                                                                                      aaTGAAGAAGTCGCCTCGCTGGGCCTCAACCTGCGCCGGATCGACGCTGTGCGCTCAGCCTTCTACCAGGCTGAATGGCAGACGGCGCACATGCTACGCCAGCGCTTCGTCGACCTCGACATCAACAGCATCGTCGACGAACTGCTGa                                                                <  5:25017/148‑1 (MQ=255)                                                                                      aaTGAAGAAGTCGCCTCGCTGGGCCTCAACCTGCGCCGGATCGACGCTGTGCGCTCAGCCTTCTACCAGGCTGAATGGCAGACGGCGCACATGCTACGCCAGCGCTTCGTCGACCTCGACATCAACAGCATCGTCGACGAACTGCTGAg                                                               >  8:108682/1‑149 (MQ=255)                                                                                      aaTGAAGAAGTCGCCTCGCTGGGCCTCAACCTGCGCCGGATCGACGCTGTGCGCTCAGCCTTCTACCAGGCTGAATGGCAGACGGCGCACATGCTACGCCAGCGCTTCGTCGACCTCGACATCAACAGCATCGTCGACGAACTGCTGAg                                                               >  4:118643/1‑149 (MQ=255)                                                                                       aTGAAGAAGTCGCCTCGCTGGGCCTCAACCTGCGCCGGATCGACGCTGTGCGCTCAGCCTTCTACCAGGCTGAATGGCAGACGGCGCACATGCTACGCCAGCGCTTCGTCGACCTCGACATCAACAGCATCGTCGACGAACTGCTGAgg                                                              >  7:143273/1‑149 (MQ=255)                                                                                        tGAAGAAGTCGCCTCGCTGGGCCTCAACCTGCGCCGGATCGACGCTGTGCGCTCAGCCTTCTACCAGGCTGAATGGCAGACGGCGCACATGCTACGCCAGCGCTTCGTCGACCTCGACATCAACAGCATCGTCGACGAACTGCTGAggg                                                             <  5:204455/149‑1 (MQ=255)                                                                                        tGAAGAAGTCGCCTCGCTGGGCCTCAACCTGCGCCGGATCGACGCTGTGCGCTCAGCCTTCTACCAGGCTGAATGGCAGACGGCGCACATGCTACGCCAGCGCTTCGTCGACCTCGACATCAACAGCATCGTCGACGAACTGCTGAggg                                                             >  8:24028/1‑149 (MQ=255)                                                                                          aagaagTCGCCTCGCTGGGCCTCAACCTGCGCCGGATCGACGCTGTGCGCTCAGCCTTCTACCAGGCTGAATGGCAGACGGCGCACATGCTACGCCAGCGCTTCGTCGACCTCGACATCAACAGCATCGTCGACGAACTGCTGAGGGt                                                            <  1:252186/148‑1 (MQ=255)                                                                                          aagaagTCGCCTCGCTGGGCCTCAACCTGCGCCGGATCGACGCTGTGCGCTCAGCCTTCTACCAGGCTGAATGGCAGACGGCGCACATGCTACGCCAGCGCTTCGTCGACCTCGACATCAACAGCATCGTCGACGAACTGCTGAGGGTc                                                           >  5:2182/1‑149 (MQ=255)                                                                                            gaagTCGCCTCGCTGGGCCTCAACCTGCGCCGGATCGACGCTGTGCGCTCAGCCTTCTACCAGGCTGAATGGCAGACGGCGCACATGCTACGCCAGCGCTTCGTCGACCTCGACATCAACAGCATCGTCGACGAACTGCTGAGGGTCGt                                                         >  4:25715/1‑149 (MQ=255)                                                                                             aagTCGCCTCGCTGGGCCTCAACCTGCGCCGGATCGACGCTGTGCGCTCAGCCTTCTACCAGGCTGAATGGCAGACGGCGCACATGCTACGCCAGCGCTTCGTCGACCTCGACATCAACAGCATCGTCGACGAACTGCTGAGGGTCGTg                                                        <  3:212287/149‑1 (MQ=255)                                                                                                tCGCCTCGCTGGGCCTCAACCTGCGCCGGATCGACGCTGTGCGCTCAGCCTTCTACCAGGCTGAATGGCAGACGGCGCACATGCTACGCCAGCGCTTCGTCGACCTCGACATCAACAGCATCGTCGACGAACTGCTGAGGGTCGTgcag                                                     <  8:108216/149‑1 (MQ=255)                                                                                                tCGCCTCGCTGGGCCTCAACCTGCGCCGGATCGACGCTGTGCGCTCAGCCTTCTACCAGGCTGAATGGCAGACGGCGCACATGCTACGCCAGCGCTTCGTCGACCTCGACATCAACAGCATCGTCGACGAACTGCTGAGGGTCGTgcag                                                     >  7:131015/1‑149 (MQ=255)                                                                                                  gCCTCGCTGGGCCTCAACCTGCGCCGGATCGACGCTGTGCGCTCAGCCTTCTACCAGGCTGAATGGCAGACGGCGCACATGCTACGCCAGCGCTTCGTCGACCTCGACATCAACAGCATCGTCGACGAACTGCTGAGGGTCGTgcag                                                     >  4:347087/1‑147 (MQ=255)                                                                                                    cTCGCTGGGCCTCAACCTGCGCCGGATCGACGCTGTGCGCTCAGCCTTCTACCAGGCTGAATGGCAGACGGCGCACATGCTACGCCAGCGCTTCGTCGACCTCGACATCAACAGCATCGTCGACGAACTGCTGAGGGTCGTGCAGCAGa                                                 <  3:271864/149‑1 (MQ=255)                                                                                                       gCTGGGCCTCAACCTGCGCCGGATCGACGCTGTGCGCTCAGCCTTCTACCAGGCTGAATGGCAGACGGCGCACATGCTACGCCAGCGCTTCGTCGACCTCGACATCAACAGCATCGTCGACGAACTGCTGAGGGTCGTGCAGCAGATgg                                              <  6:2182/149‑1 (MQ=255)                                                                                                         tGGGCCTCAACCTGCGCCGGATCGACGCTGTGCGCTCAGCCTTCTACCAGGCTGAATGGCAGACGGCGCACATGCTACGCCAGCGCTTCGTCGACCTCGACATCAACAGCATCGTCGACGAACTGCTGAGGGTCGTGCAGCAGATGGc                                             >  1:160255/1‑148 (MQ=255)                                                                                                           ggCCTCAACCTGCGCCGGATCGACGCTGTGCGCTCAGCCTTCTACCAGGCTGAATGGCAGACGGCGCACATGCTACGCCAGCGCTTCGTCGACCTCGACATCAACAGCATCGTCGACGAACTGCTGAGGGTCGTGCAGCAGATGGCga                                           <  2:103777/148‑1 (MQ=255)                                                                                                             ccTCAACCTGCGCCGGATCGACGCTGTGCGCTCAGCCTTCTACCAGGCTGAATGGCAGACGGCGCACATGCTACGCCAGCGCTTCGTCGACCTCGACATCAACAGCATCGTCGACGAACTGCTGAGGGTCGTGCAGCAGATGGCgatga                                        >  7:325927/1‑149 (MQ=255)                                                                                                              cTCAACCTGCGCCGGATCGACGCTGTGCGCTCAGCCTTCTACCAGGCTGAATGGCAGACGGCGCACATGCTACGCCAGCGCTTCGTCGACCTCGACATCAACAGCATCGTCGACGAACTGCTGAGGGTCGTGCAGCAGATGGCgatgat                                       >  5:261000/1‑149 (MQ=255)                                                                                                                 aaCCTGCGCCGGATCGACGCTGTGCGCTCAGCCTTCTACCAGGCTGAATGGCAGACGGCGCACATGCTACGCCAGCGCTTCGTCGACCTCGACATCAACAGCATCGTCGACGAACTGCTGAGGGTCGTGCAGCAGATGGCGATGAtcgt                                    >  5:104314/1‑149 (MQ=255)                                                                                                                     tGCGCCGGATCGACGCTGTGCGCTCAGCCTTCTACCAGGCTGAATGGCAGACGGCGCACATGCTACGCCAGCGCTTCGTCGACCTCGACATCAACAGCATCGTCGACGAACTGCTGAGGGTCGTGCAGCAGATGGCGATGAtcgtcgtc                                >  7:96875/1‑149 (MQ=255)                                                                                                                      gcgcCGGATCGACGCTGTGCGCTCAGCCTTCTACCAGGCTGAATGGCAGACGGCGCACATGCTACGCCAGCGCTTCGTCGACCTCGACATCAACAGCATCGTCGACGAACTGCTGAGGGTCGTGCAGCAGATGGCGATGAtcgtcgtcg                               >  4:37323/1‑149 (MQ=255)                                                                                                                       cgcCGGATCGACGCTGTGCGCTCAGCCTTCTACCAGGCTGAATGGCAGACGGCGCACATGCTACGCCAGCGCTTCGTCGACCTCGACATCAACAGCATCGTCGACGAACTGCTGAGGGTCGTGCAGCAGATGGCGATGAtcgtcgtcg                               <  7:24028/148‑1 (MQ=255)                                                                                                                       cgcCGGATCGACGCTGTGCGCTCAGCCTTCTACCAGGCTGAATGGCAGACGGCGCACATGCTACGCCAGCGCTTCGTCGACCTCGACATCAACAGCATCGTCGACGAACTGCTGAGGGTCGTGCAGCAGATGGCGATGAtcgtcgtc                                <  4:183822/147‑1 (MQ=255)                                                                                                                        gcCGGATCGACGCTGTGCGCTCAGCCTTCTACCAGGCTGAATGGCAGACGGCGCACATGCTACGCCAGCGCTTCGTCGACCTCGACATCAACAGCATCGTCGACGAACTGCTGAGGGTCGTGCAGCAGATGGCgatgat                                       >  7:115677/1‑139 (MQ=255)                                                                                                                        gcCGGATCGACGCTGTGCGCTCAGCCTTCTACCAGGCTGAATGGCAGACGGCGCACATGCTACGCCAGCGCTTCGTCGACCTCGACATCAACAGCATCGTCGACGAACTGCTGAGGGTCGTGCAGCAGATGGCgatgat                                       <  8:115677/139‑1 (MQ=255)                                                                                                                        gcCGGATCGACGCTGTGCGCTCAGCCTTCTACCAGGCTGAATGGCAGACGGCGCACATGCTACGCCAGCGCTTCGTCGACCTCGACATCAACAGCATCGTCGACGAACTGCTGAGGGTCGTGCAGCAGATGGCGATGATCGTCGTCgg                              >  1:60407/1‑148 (MQ=255)                                                                                                                         ccGGATCGACGCTGTGCGCTCAGCCTTCTACCAGGCTGAATGGCAGACGgc                                                                                                                              >  8:332008/1‑51 (MQ=255)                                                                                                                         ccGGATCGACGCTGTGCGCTCAGCCTTCTACCAGGCTGAATGGCAGACGgc                                                                                                                              <  7:332008/51‑1 (MQ=255)                                                                                                                                 aCGCTGTGCGCTCAGCCTTCTACCAGGCTGAATGGCAGACGGCGCACATGCTACGCCAGCGCTTCGTCGACCTCGACATCAACAGCATCGTCGACGAACTGCTGAGGGTCGTGCAGCAGATGGCg                                            <  6:384202/125‑1 (MQ=255)                                                                                                                                 aCGCTGTGCGCTCAGCCTTCTACCAGGCTGAATGGCAGACGGCGCACATGCTACGCCAGCGCTTCGTCGACCTCGACATCAACAGCATCGTCGACGAACTGCTGAGGGTCGTGCAGCAGATGGCg                                            >  5:384202/1‑125 (MQ=255)                                                                                                                                 aCGCTGTGCGCTCAGCCTTCTACCAGGCTGAATGGCAGACGGCGCACATGCTACGCCAGCGCTTCGTCGACCTCGACATCAACAGCATCGTCGACGAACTGCTGAGGGTCGTGCAGCAGATGGCGATGATCGTCGTCGGCAGCACCGt                     >  7:175399/1‑148 (MQ=255)                                                                                                                                    cTGTGCGCTCAGCCTTCTACCAGGCTGAATGGCAGACGGCGCACATGCTACGCCAGCGCTTCGTCGACCTCGACATCAACAGCATCGTCGACGAACTGCTGAGGGTCGTGCAGCAGATGGCGATGATCGTCGTCGGCAGCACCGTTAcc                 <  6:261000/149‑1 (MQ=255)                                                                                                                                     tgtgCGCTCAGCCTTCTACCAGGCTGAATGGCAGACGGCGCACATGCTACGCCAGCGCTTCGTCGACCTCGACATCAACAGCATCGTCGACGAACTGCTGAGGGTCGTGCAGCAGATGGCGATGAtcgtc                                   <  5:3066/130‑1 (MQ=255)                                                                                                                                     tgtgCGCTCAGCCTTCTACCAGGCTGAATGGCAGACGGCGCACATGCTACGCCAGCGCTTCGTCGACCTCGACATCAACAGCATCGTCGACGAACTGCTGAGGGTCGTGCAGCAGATGGCGATGAtcgtc                                   >  6:3066/1‑130 (MQ=255)                                                                                                                                       tgCGCTCAGCCTTCTACCAGGCTGAATGGCAGACGGCGCACATGCTACGCCAGCGCTTCGTCGACCTCGACATCAACAGCATCGTCGACGAACTGCTGAGGGTCGTGCAGCAGATGGCGATGATCGTCGTCGGCAGCACCGTTACCGGt              <  2:160255/149‑1 (MQ=255)                                                                                                                                       tgCGCTCAGCCTTCTACCAGGCTGAATGGCAGACGGCGCACATGCTACGCCAGCGCTTCGTCGACCTCGACATCAACAGCATCGTCGACGAACTGCTGAGGGTCGTGCAGCAGATGGCGATGATCGTCGTCGGCAGCACCGTTACCGGt              >  2:141813/1‑149 (MQ=255)                                                                                                                                       tgCGCTCAGCCTTCTACCAGGCTGAATGGCAGACGGCGCACATGCTACGCCAGCGCTTCGTCGACCTCGACATCAACAGCATCGTCGACGAACTGCTGAGGGTCGTGCAGCAGATGGCGATGATCGTCGTCGGCAGCACCGTTACCGGt              <  8:175399/149‑1 (MQ=255)                                                                                                                                         cgcTCAGCCTTCTACCAGGCTGAATGGCAGACGGCGCACATGCTACGCCAGCGCTTCGTCGACCTCGACATCAACAGCATCGTCGACGAACTGCTGAGGGTCGTGCAGCAGATGGCGATGATCGTCGTCGGCAGCACCGTTACCGGTgg            >  8:332785/1‑149 (MQ=255)                                                                                                                                           ttCAGCCTGGTAGAAGGCTGAATGGCAGACGGCGCACATGCTACGCCAGCGCTTCGTCGACCTCGACATCAACAGCATCGTCGACGAACTGCTGAGGGTCGTGCAGCAGATGGCGATGATCGTCGTCGGCAGCACCGTTACCg                <  7:322879/142‑1 (MQ=255)                                                                                                                                           ttCAGCCTGGTAGAAGGCTGAATGGCAGACGGCGCACATGCTACGCCAGCGCTTCGTCGACCTCGACATCAACAGCATCGTCGACGAACTGCTGAGGGTCGTGCAGCAGATGGCGATGATCGTCGTCGGCAGCACCGTTACCg                >  8:322879/2‑143 (MQ=255)                                                                                                                                              aGCCTTCTACCAGGCTGAATGGCAGACGGCGCACATGCTACGCCAGCGCTTCGTCGACCTCGACATCAACAGCATCGTCGACGAACTGCTGAGGGTCGTGCAGCAGATGGCGATGATCGTCGTCGGCAGCACCGTTACCGGTGGCCTGc       <  1:170630/149‑1 (MQ=255)                                                                                                                                                ccTTCTACCAGGCTGAATGGCAGACGGCGCACATGCTACGCCAGCGCTTCGTCGACCTCGACATCAACAGCATCGTCGACGAACTGCTGAGGGTCGTGCAGCAGATGGCGATGATCGTCGTCGGCAGCACCGTTACCGGTGGCCTGCtt     <  8:275118/149‑1 (MQ=255)                                                                                                                                                 cTTCTACCAGGCTGAATTGAAGACGGCGCACATGCTACGCCAGCGCTTCGTCGACCTCGACATCAACAGCATCTTCGACGAACTGCTGAGGGTCGTGCAGCATATGGCGATGATCGTCGTCGGCAGCACCGTTACCGGTTGCCTGCTTg    <  6:353958/149‑1 (MQ=14)                                                                                                                                                  ttCTACCAGGCTGAATGGCAGACGGCGCACATGCTACGCCAGCGCTTCGTCGACCTCGACATCAACAGCATCGTCGACGAACTGCTGAGGGTCGTGCAGCAGATGGCGATGATCGTCGTCGGCAGCACCGTTACCGGTGGCCTGCTTgg   >  5:111482/1‑149 (MQ=255)                                                                                                                                                   tCTACCAGGCTGAATGGCAGACGGCGCACATGCTACGCCAGCGCTTCGTCGACCTCGACATCAACAGCATCGTCGACGAACTGCTGAGGGTCGTGCAGCAGATGGCGATGATCGTCGTCGGCAGCACCGTTACCGGTGGCCTGCTTGgc  >  7:146003/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                     CCTCGTGCCCTCCTGGCACGAGATCGAGTCACGTGTCATCGCCATGGTGGGCTACCCAAGCGGTGGCCAGTTCCGCACCAGCCTCAATGAAGAAGTCGCCTCGCTGGGCCTCAACCTGCGCCGGATCGACGCTGTGCGCTCAGCCTTCTACCAGGCTGAATGGCAGACGGCGCACATGCTACGCCAGCGCTTCGTCGACCTCGACATCAACAGCATCGTCGACGAACTGCTGAGGGTCGTGCAGCAGATGGCGATGATCGTCGTCGGCAGCACCGTTACCGGTGGCCTGCTTGGC  >  NC_002947/898149‑898443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑