Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	941,933	A→G	5.6%	V1230A (GTC→GCC)	PP_0806 ←	surface adhesion protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	941,933	0	A	G	5.6%	272.5 / 4.6	93	V1230A (GTC→GCC)	PP_0806	surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base A (42/43);  new base G (2/3);  total (46/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.85e-01

GGTGACCGCCTCGGGCGGTGTGATATCCGGGGCTGTGAACTCCAGCGGTATCGAACTGTTGCCGGCGGCATCCACCAGACGCACGTCGAGTGCTTCACCATTGGCCTGGGCCGGCGACAGGGTGAGGCTGAACAGGCCGTCGGCACCGACGACGCCGGTGCCAATTAGGTTGCCGGCAGCATCGCGCACTTGCACGGTAGCGCCGGGCTCGCCGCGACCGGTGAAAGTGACGCCATCGGCCAAGGCCAGGTCAGTCGGTTGTGCAGGCGGCAGCAAGTCGGCCGAGGTGACCGAGC  >  NC_002947/941785‑942080                                                                                                                                                     |                                                                                                                                                    ggTGACCGCCTCGGGCGGTGTGATATCCGGGGCTGTGAACTCCAGCGGTATCGAACTGTTGCCGGCGGCATCCACCAGACGCACGTCGAGTGCTTCACCATTGGCCTGGGCCGGCGACAGGGTGAGGCTGAACAGGCCGTCGGCACcga                                                                                                                                                     >  1:253822/1‑149 (MQ=37)       cGCCTCGGGCGGTGTGATATCCGGGGCTGTGAACTCCAGCGGTATCGAACTGTTGCCGGCGGCATCCACCAGACGCACGTCGAGTGCTTCACCATTGGCCTGGGCCGGCGACAGGGTGAGGCTGAACAGGCCGTCGGCACCGACGACGc                                                                                                                                               >  2:83060/1‑149 (MQ=38)        gCCTCGGGCGGTGTGATATCCGGGGCTGTGAACTCCAGCGGTATCGAACTGTTGCCGGCGGCATCCACCAGACGCACGTCGAGTGCTTCACCATTGGCCTGGGCCGGCGACAGGGTGAGGCTGAACAGGCCGTCGGCACCGgcgtcgtc                                                                                                                                              >  8:84286/1‑147 (MQ=255)          cTCGGGCGGTGTGATATCCGGGGCTGTGAACTCCAGCGGTATCGAACTGTTGCCGGCGGCATCCACCAGACGCACGTCGAGTGCTTCACCATTGGCCTGGGCCGGCGACAGGGTGAGGCTGAACAGGCCGTCGGCACCGACGACGCCgg                                                                                                                                            <  5:142671/149‑1 (MQ=39)             gggCGGTGTGATATCCGGGGCTGTGAACTCCAGCGGTATCGAACTGTTGCCGGCGGCATCCACCAGACGCTCGTCGAGTGCTTCACCATTGGCCTGGGCCGGCGACAGGGTGAGGCTGAACAGGCCGTCGGCACCGACGACGCCGGTGc                                                                                                                                         <  1:102469/149‑1 (MQ=255)             gggCGGTGTGATATCCGGGGCTGTGAACTCCAGCGGTATCGAACTGTTGCCGGCGGCATCCACCAGACGCACGTCGAGTGCTTCACCATTGGCCTGGGCCGGCGACAGGGTGAGGCTGAACAGGCCGTCGGCACCGGCGTCGTCGGTGc                                                                                                                                         <  7:285673/149‑1 (MQ=255)             gggCGGTGTGATATCCGGGGCTGTGAACTCCAGCGGTATCGAACTGTTGCCGGCGGCATCCACCAGACGCACGTCGAGTGCTTCACCATTGGCCTGGGCCGGCGACAGGGTGAGGCTGAACAGGCCGTCGGCACCGACGACGCCGGt                                                                                                                                           <  8:216702/147‑1 (MQ=39)             gggCGGTGTGATATCCGGGGCTGTGAACTCCAGCGGTATCGAACTGTTGCCGGCGGCATCCACCAGACGCACGTCGAGTGCTTCACCATTGGCCTGGGCCGGCGACAGGGTGAGGCTGAACAGGCCGTCGGCACCGACGACGCCGGt                                                                                                                                           <  4:321164/147‑1 (MQ=39)             gggCGGTGTGATATCCGGGGCTGTGAACTCCAGCGGTATCGAACTGTTGCCGGCGGCATCCACCAGACGCACGTCGAGTGCTTCACCATTGGCCTGGGCCGGCGACAGGGTGAGGCTGAACAGGCCGTCGGCACCGACGACGCCGGTg                                                                                                                                          <  7:35007/148‑1 (MQ=40)                cGGTGTGATATCCGGGGCTGTGAACTCCAGCGGTATCGAACTGTTGCCGGCGGCATCCACCAGACGCACGTCGAGTGCTTCACCATTGGCCTGGGCCGGCGACAGGGTGAGGCTGAACAGGCCGTCGGCACCGGCGTCGTCGGTGccga                                                                                                                                      <  7:84286/149‑3 (MQ=255)                 ggTGTGATATCCGGGGCTGTGAACTCCAGCGGTATCGAACTGTTGCCGGCGGCATCCACCAGACGCACGTCGAGTGCTTCACCATTGGCCTGGGCCGGCGACAGGGTGAGGCTGAACAGGCCGTTGGCACCGGCGTCGTCGGTGccgac                                                                                                                                     <  7:262416/149‑4 (MQ=11)                       atatCCGGGGCTGTGAACTCCAGCGGTATCGAACTGTTGCCGGCGGCATCCACCAGACGCACGTCGAGTGCTTCACCATTGGCCTGGGCCGGCGACAGGGTGAGGCTGAACAGGCCGTCGGCACCGACGACGCCGGTGCCAATTAGGtt                                                                                                                               >  5:344056/1‑149 (MQ=255)                         atCCGGGGCTGTGAACTCCAGCGGAATCGAACTGTTGCCGGCGGCATCCACCAGACGCACGTCGAGTGCTTCACCATTGGCCTGGGCCGGCGACAGGGTGAGGCTGAACAGGCCGTCGGCACCGACGACGCCGGTGCCAATTAGGTTGc                                                                                                                             >  3:60685/1‑149 (MQ=255)                          tCCGGGGCTGTGAACTCCAGCGGTATCGAACTGTTGCCGGCGGCATCCACCAGACGCACGTCGAGTGCTTCACCATTGGCCTGGGCCGGCGACAGGGTGAGGCTGAACAGGCCGTCGGCACCGACGACGCCGGTGCCAATTAGGTTGcc                                                                                                                            >  4:368314/1‑149 (MQ=255)                             ggggCTGTGAACTCCAGCGGTATCGAACTGTTGCCGGCGGCATCCACCAGACGCACGTCGAGTGCTTCACCATTGGCCTGGGCCGGCGACAGGGTGAGGCTGAACAGGCCGTCGGCACCGACGACGCCGGTGCCAATTAGGTTGCCgg                                                                                                                          >  7:16351/1‑148 (MQ=255)                                gCTGTGAACTCCAGCGGTATCGAACTGTTGCCGGCGGCATCCACCAGACGCACGTCGAGTGCTTCACCATTGGCCTGGGCCGGCGACAGGGTGAGGCTGAACAGGCCGTCGGCACCGACGACGCCGGTGCCAATTAGGTTGCCGgcag                                                                                                                       >  4:181242/1‑148 (MQ=255)                                  tgtgAACTCCAGCGGTATCGAACTGTTGCCGGCGGCATCCACCAGACGCACGTCGAGTGCTTCACCATTGGCCTGGGCCGGCGACAGGGTGAGGCTGAACAGGCCGTCGGCACCGACGACGCCGGTGCCAATTAGGTTGCCGGCAGCAt                                                                                                                    >  1:125461/1‑149 (MQ=255)                                  agtgAACTCCAGCGGTATCGAACTGTTGCCGGCGGCATCCACCAGACGCACGTCGAGTGCTTCACCAATGGCCTGGGACGGCGACAGGGTGAGGCTGAACAGGCCGTCGGCACCGCCGACGCCCGTGCCAAATAGGATGCCGGCAGCAt                                                                                                                    >  6:256517/2‑149 (MQ=17)                                    tgAACTCCAGCGGTATCGAACTGTTGCCGGCGGCATCCACCAGACGCACGTCGAGTGCTTCACCATTGGCCTGGGCCGGCGACAGGGTGAGGCTGAACAGGCCGTCGGCACCGACGACGCCGGTGCCAATTAGGTTGCCGGCAGCATcg                                                                                                                  >  8:80177/1‑149 (MQ=255)                                        cTCCAGCGGTATCGAACTGTTGCCGGCGGCATCCACCAGACGCACGTCGAGTGCTTCACCATTGGCCTGGGCCGGCGACAGGGTGAGGCTGAACAGGCCGTCGGCACCGACGACGCCGGTGCCAATTAGGTTGCCGGCAGCATCGCGCa                                                                                                              >  3:74278/1‑149 (MQ=255)                                        cTCCAGCGGTATCGAACTGTTGCCGGCGGCATCCACCAGACGCACGTCGAGTGCTTCACCATTGGCCTGGGCCGGCGACAGGGTGAGGCTGAACAGGCCGTCGGCACCGACGACGCCGGTGCCAATTAGGTTGCCGGCAGCATCGCGCa                                                                                                              <  1:148450/149‑1 (MQ=255)                                         tCCAGCGGTATCGAACTGTTGCCGGCGGCATCCACCAGACGCACGTCGAGTGCTTCACCATTGGCCTGGGCCGGCGACAGGGTGAGGCTGAACAGGCCGTCGGCACCGACGACGCCGGTGCCAATTAGGTTGCCGGCAGCATCGCGCAc                                                                                                             >  3:294728/1‑149 (MQ=255)                                          ccAGCGGTATCGACCTGTTGCCGGCGGCATCCACCAGACGCACGTCGAGTGCTTCACCATTGGCCTGGGCCGGCGACAGGGTGAGGCTGAACAGGCCGTCGGCACCGACGACGCCGGTGCCAATTAGGTTGCCGGCAGCATCGCGCACt                                                                                                            <  4:38891/149‑1 (MQ=255)                                              cGGTATCGAACTGTTGCCGGCGGCATCCACCAGACGCACGTCGAGTGCTTCACCATTGGCCTGGGCCGGCGACAGGGTGAGGCTGAACAGGCCGTCGGCACCGTCGACGCCGGTGCCAATTAGGTTGCCGGCAGCATCGCGCACTTGCa                                                                                                        <  1:83060/149‑1 (MQ=255)                                                  aTCGAACTGTTGCCGGCGGCATCCACCAGACGCACGTCGAGTGCTTCACCATTGACCTGGGCCGGCGACAGGGTGAGGCTGAACAGGCCGTCGGCACCGACGACGCCGGTGCCAATTAGGTTGCCGGCAGCATCGCGCACTTGCACgg                                                                                                     >  1:288630/1‑148 (MQ=255)                                                     gAACTGTTGCCGGCGGCATCCACCAGACGCACGTCGAGTGCTTCACCATTGGCCTGGGCCGGCGACAGGGTGAGGCTGAACAGGCCGTCGGCACCGACGACGCCGGTGCCAATTAGGTTGCCGGCAGCATCGCGCACTTGCACGGTAgc                                                                                                 <  3:368314/149‑1 (MQ=255)                                                      aaCTGTTGCCGGCGGCATCCACCAGACGCACGTCGAGTGCTTCACCATTGGCCTGGGCCGGCGACAGGGTGAGGCTGAACAGGCCGTCGGCACCGACGACGCCGGTGCCAATTAGGTTGCCGGCAGCATCGCGCACTTGCACGGTAgcg                                                                                                >  8:135395/1‑149 (MQ=255)                                                         tGTTGCCGGCGGCATCCACCAGACGCACGTCGAGTGCTTCACCATTGGCCTGGGCCGGCGACAGGGTGAGGCTGAACAGGCCGTCGGCACCGACGACGCCGGTGCCAATTAGGTTGCCGGCAGCATCGCGCACTTGCACGGTAGCGCCg                                                                                             >  7:314587/1‑149 (MQ=255)                                                           ttGCCGGCGGCATCCACCAGACGCACGTCGAGTGCTTCACCATTGGCCTGGGCCGGCGACAGGGTGAGGCTGAACAGGCCGTCGGCACCGACGACGCCGGTGCCAATTAGGTTGCCGGCAGCATCGCGCACTTGCACGGTAGCGCCgg                                                                                            <  7:80177/148‑1 (MQ=255)                                                           ttGCCGGCGGCATCCACCAGACGCACGGCGAGTGCTTCACCATTGGCCTGGGCCGGCGACAGGGTGAGGCTGAACAGGCCGTCGGCACCGACGACGCCGGTGCCAATTAGGGTGCCGGCAGCATCGCGCACTTGCACGGTAGCGCCgg                                                                                            <  2:120997/148‑1 (MQ=255)                                                              ccGGCGGCATCCAACAGACGCACGTCGAGTGCTTCCCCATTGGCCTGGGCCGGCGACAGGGTGAGGCTGAACAGGCCGTCGGCACCGACGACGCCGGTGCCAATTAGGTTGCCGGCAGCATCGCGCACTTGCACGGTAGCGCCGGGCTc                                                                                        >  6:187142/1‑149 (MQ=255)                                                                 gcggcATCCACCAGACGCACGTCGAGTGCTTCACCATTGGCCTGGGCCGGCGACAGGGTGAGGCTGAACAGGCCGTCGGCACCGACGACGCCGGTGCCAATTAGGTTGCCGGCAGCATCGCGCACTTGCACGGTAGCGCCGGGCTcgcc                                                                                     <  8:16351/149‑1 (MQ=255)                                                                 gcggcATCCACCAGACGCACGTCGAGTGCTTCACCATTGGCCTGGGCCGGCGACAGGGTGAGGCTGAACAGGCCGTCGGCACCGACGACGCCGGTGCCAATTAGGTTGCCGGCAGCATCGCGCACTTGCACGGTAGCGCCGGGCTcgcc                                                                                     >  7:107962/1‑149 (MQ=255)                                                                   ggcATCCACCAGACGCACGTCGAGTGCTTCACCAATGGCCTGGGCCGGCGACAGGGTGAGGCTGAACAGACCGTCGGCACCGACGACGCCGGTGCCAATTAGGATGCCGGCAGCATCGCGCACTTGCACGGTAGCGCCGGGCTcgccgc                                                                                   <  6:192816/149‑1 (MQ=255)                                                                     cATCCACCAGACGCACGTCGAGTGCTTCACCATTGGCCTGGGCCGGCGACAGGGTGAGGCTGAACAGGCCGTCGGCACCGACGACGCCGGTGCCAATTAGGTTGCCGGCAGCATCGCGCACTTGCACGGTAGCGCCGGGCTCGCCGCGa                                                                                 >  2:353366/1‑149 (MQ=255)                                                                      aTCCACCAGACGCACGTCGAGTGCTTCACCTTTGGCCTGGGCCGGCGACAGGGTGAGGCTGAACAGGCCGTCGGCACCGACGACGCCGGTGCCAATTAGGTTGCCGGCAGCATc                                                                                                                   >  1:33583/1‑114 (MQ=255)                                                                      aTCCACCAGACGCACGTCGAGTGCTTCACCATTGGCCTGGGCCGGCGACAGGGTGAGGCTGAACAGGCCGTCGGCACCGACGACGCCGGTGCCAATTAGGTTGCCGGCAGCATc                                                                                                                   <  2:33583/114‑1 (MQ=255)                                                                       tcaaccaGACGCACGTCGAGTGCTTCACCATTGGCCTGGGCCGGCGACAGGGTGAGGCTGAACAGGCCGTCGGCACCGACGACGCCg                                                                                                                                             >  2:337972/4‑87 (MQ=255)                                                                       tcaaccaGACGCACGTCGAGTGCTTCACCATTGGCCTGGGCCGGCGACAGGGTGAGGCTGAACAGGCCGTCGGCACCGACGACGCCg                                                                                                                                             <  1:337972/84‑1 (MQ=255)                                                                       tCCACCAGACGCACGTCGAGTGCTTCACCATTGGCCTGGGCCGGCGACAGGGTGAGGCTGAACAGGCCGTCGGCACCGACGACGCCGGTGCCAATTAGGTTGCCGGCAGCATCGCGCACTTGCACGGTAGCGCCGGGCTCGCCGCGAcc                                                                               <  5:132799/149‑1 (MQ=255)                                                                         caccaGACGCACGTCGAGTGCTTCACCATTGGCCTGGGCCGGCGACAGGGTGAGGCTGAACAGGCCGTCGGCACCGACGACGCCGGTGCCAATTAGGTTGCCGGCAGCATCGCGCACTTGCACGGTAGCGCCGGGCTCGCCGCGACCgg                                                                             >  5:238109/1‑149 (MQ=255)                                                                         caccaGACGCACGTCGAGTGCTTCACCATTGGCCTGGGCCGGCGACAGGGTGAGGCTGAACAGGCCGTCGGCACCGACGACGCCGGTGCCAATTAGGTTGCCGGCAGCATCGCGCACTTGCACGGTAGCGCCGGGCTCGCCGCGACCg                                                                              <  7:135395/148‑1 (MQ=255)                                                                          accaGACGCACGTCGAGTGCTTCACCATTGGCCTGGGCCGGCGACAGGGTGAGGCTGAACAGGCCGTCGGCACCGACGACGCCGGTGCCAATTAGGTTGCCGGCAGCATCGCGCACTTGCACGGTAGCGCCGGGCTCGCCGCGACCGGt                                                                            >  2:378678/1‑149 (MQ=255)                                                                           ccaGACGCACGTCGAGTGCTTCACCATTGGCCTGGGCCGGCGACAGGGTGAGGCTGAACAGGCCGTCGGCACCGACGACGCCGGTGCCAATTAGGTTGCCGGCAGCATCGCGCACTTGCACGGTAGCGCCGGGCTCGCCGCGACCGGt                                                                            >  2:95132/1‑148 (MQ=255)                                                                                     ggcGAGTGCTTCTCCATTGGCCTGGGCCGGCGACAGGGTGAGGCTGAACAGGCCGTCGGCAGCGACGACGCCGGTGCCAATTAGGTTGCCGGCAGCATCGCGCACTTGCACGGTAGCGCCGGGCTCGCCGCGACCGGTGAAAGTGACGc                                                                 <  5:256517/147‑1 (MQ=255)                                                                                     gTCGAGTGCTTCACCATTGGCCTGGGCCGGCGACAGGGTGAGGCTGAACAGGCCGTCGGCACCGACGACGCCGGTGCCAATTAGGTTGCCGGCAGCATCGCGCACTTGCACGGTAGCGCCGGGCTCGCCGCGACCGGTGaa                                                                         <  4:39258/141‑1 (MQ=255)                                                                                     gTCGAGTGCTTCACCATTGGCCTGGGCCGGCGACAGGGTGAGGCTGAACAGGCCGTCGGCACCGACGACGCCGGTGCCAATTAGGTTGCCGGCAGCATCGCGCACTTGCACGGTAGCGCCGGGCTCGCCGCGACCGGTGaa                                                                         >  3:39258/1‑141 (MQ=255)                                                                                          gTGCTTCACCATTGGCCTGGGCCGGCGACAGGGTGAGGCTGAACAGGCCGTCGGCACCGACGACGCCGGTGCCAATTAGGTTGCCGGCAGCATCGCGCACTTGCACGGTAGCGCCGGGCTCGCCGCGACCGGTGAAAGTGACGCCATCg                                                            <  5:4216/149‑1 (MQ=255)                                                                                          gTGCTTCACCATTGGCCTGGGCCGGCGACAGGGTGAGGCTGAACAGGCCGTCGGCACCGACGACGCCGGTGCCAATTAGGTTGCCGGCAGCATCGCGCACTTGCACGGTAGCGCCGGGCTCGCCGCGACCGGTGAAAGTGACGCCATCg                                                            >  1:128751/1‑149 (MQ=255)                                                                                           tGCTTCACCATTGGCCTGGGCCGGCGACAGGGTGAGGCTGAACAGGCCGTCGGCACCGACGACGCCGGTGCCAATTAGGTTGCCGGCAGCATCGCGCACTTGCACGGTAGCGCCGGGCTCGCCGCGACCGGTGAAAGTGACGCCATCg                                                            <  6:344056/148‑1 (MQ=255)                                                                                             cTTCACCATTGGCCTGGGCCGGCGACAGGGTGAGGCTGAACAGGCCGTCGGCACCGCCGCCGCCGGTGCCAATTAGGTTGCCGGCAGCATCGCGCACTTGCACGGTAGCGCCGGGCTCGCCGCGACCGGTGAAAGTGACGCCATCGGc                                                          >  2:163921/1‑148 (MQ=255)                                                                                              ttCACCATTGGCCTGGGCCGGCGACAGGGTGAGGCTGAACAGGCCGTCGGCACCGACGACGCCGGTGCCAATTAGGTTGCCGGCAGCATCGCGCACTTGCACGGTAGCGCCGGGCTCGCCGCGACCGGTGAAAGTGACg                                                                  >  1:144413/1‑139 (MQ=255)                                                                                              ttCACCATTGGCCTGGGCCGGCGACAGGGTGAGGCTGAACAGGCCGTCGGCACCGACGACGCCGGTGCCAATTAGGTTGCCGGCAGCATCGCGCACTTGCACGGTAGCGCCGGGCTCGCCGCGACCGGTGAAAGTGACg                                                                  <  2:144413/139‑1 (MQ=255)                                                                                              ttCACCATTGGCCTGGGCCGGCGACAGGGTGAGGCTGAACAGGCCGTCGGCACCGACGACGCCGGTGCCAATTAGGTTGCCGGCAGCATCGCGCACTTGCACGGTAGCGCCGGGCTCGCCGCGACCGGTGAAAGTGACGCCATCGGcc                                                         >  7:146444/1‑148 (MQ=255)                                                                                              ttCACCATTGGCCTGGGCCGGCGACAGGGTGAGGCTGAACAGGCCGTCGGCACCGACGACGCCGGTGCCAATTAGGTTGCCGGCAGCATCGCGCACTTGCACGGTAGCGCCGGGCTCGCCGCGACCGGTGAAAGTGACGCCATCGGcc                                                         >  3:6900/1‑148 (MQ=255)                                                                                              ttCACCATTGGCCTGGGCCGGCGACAGGGTGAGGCTGAACAGGCCGTCGGCACCGACGACGCCGGTGCCAATTAGGTTGCCGGCAGCATCGCGCACTTGCACGGTAGCGCCGGGCTCGCCGCGACCGGTGAAAGTGACGCCATCGGcc                                                         >  6:148556/1‑148 (MQ=255)                                                                                              ttCACCATTGGCCTGGGCCGGCGACAGGGTGAGGCTGAACAGGCCGTCGGCACCGACGACGCCGGTGCCAATTAGGTTGCCGGCAGCATCGCGCACTTGCACGGTAGCGCCGGGCTCGCCGCGACCGGTGAAAGTGACGCCATCGGcc                                                         >  7:150827/1‑148 (MQ=255)                                                                                               tCACCATTGGCCTGGGCCGGCGACAGGGTGAGGCTGAACAGGCCGTCGGCACCGACGGCGCCGGTGCCAATTTGGTTGGCGGGAGCATAGCGCACTTGCACGGTAGCGCCGGGCTCGCCGGGACCGGGGGAAGTGACGCCCTCGGcc                                                         >  6:237005/1‑147 (MQ=17)                                                                                               tCACCATTGGCCTGGGCCGGCGACAGGGTGAGGCTGAACAGGCCGACGGCACCGACGACGCCGGTGCCAATTAGGTTGCCGGCAGCATCGCGCACTTGCACGGTAGCGCCGGGCTCGCCGCGACCGGTGAAAGTGACGCCATCGGcc                                                         >  1:268103/1‑147 (MQ=255)                                                                                                cACCATTGGCCTGGGCCGGCGACAGGGTGAGGCTGAACAGGCCGTCGGCACCGACGACGCCGGTGCCAATTAGGTTGCCGGCAGCATCGCGCACTTGCACGGTAGCGCCGGGCTCGCCGCGACCGGTGAAAGTGACGCCATCGGCCAAg                                                      >  4:37521/1‑149 (MQ=255)                                                                                                 aCCATTGGCCTGGGCCGGCGACAGGGTGAGGCTGAACAGGCCGTCGGCACCGACGACGCCGGTGCCAATTAGGTTGCCGGCAGCATCGCGCACTTGCACGGTAGCGCCGGGCTCGCCGCGACCGGTGAAAGTGACGCCATCGGCCAAg                                                      <  8:314587/148‑1 (MQ=255)                                                                                                   cATTGGCCTGGGCCGGCGACAGGGTGAGGCTGAACAGGCCGTCGGCACCGACGACGCCGGTGCCAATTAGGTTGCCGGCAGCATCGCGCACTTGCACGGTAGCGCCGGGCTCGCCGCGACCGGTGAAAGTGAc                                                                   >  3:234685/1‑133 (MQ=255)                                                                                                   cATTGGCCTGGGCCGGCGACAGGGTGAGGCTGAACAGGCCGTCGGCACCGACGACGCCGGTGCCAATTAGGTTGCCGGCAGCATCGCGCACTTGCACGGTAGCGCCGGGCTCGCCGCGACCGGTGAAAGTGAc                                                                   <  4:234685/133‑1 (MQ=255)                                                                                                   cATTGGCCTGGGCCGGCGACAGGGTGAGGCTGAACAGGCCGTCGGCACCGACGACGCCGGTGCCAATTAGGTTGCCGGCAGCATCGCGCACTTGCACGGTAGCGCCGGGCTCGCCGCGAAAGGTGAAAGTGACGCCATCGGCCAAGGc                                                    >  6:70088/1‑148 (MQ=255)                                                                                                     ttGGCCTGGGCCGGCGACAGGGTGAGGCTGAACAGGCCGTCGGCACCGACGACGCCGGTGCCAATTAGGTTGCCGGCAGCATCGCGCACTTGCACGGTAGCGCCGGGCTCGCCGCGACCGGTGAAAGTGACGCCATCGGCCAAGGCCAg                                                 <  1:353366/149‑1 (MQ=255)                                                                                                        gCCTGGGCCGGCGACAGGGTGAGGCTGAACAGGCCGTCGGCACCGACGACGCCGGTGCCAATTAGGTTGCCGGCAGCATCGCGCACTTGCACGGTAGCGCCGGGCTCGCCGCGACCGGTGAAAGTGACGCCATCGGCCAAGGCCAGGt                                               <  4:294728/148‑1 (MQ=255)                                                                                                        gCCTGGGCCGGCGACAGGGTGAGGCTGAACAGGCCGTCGGCACCGACGACGCCGGTGCCAATTAGGTTGCCGGCAGCATCGCGCACTTGCACGGTAGCGCCGGGCTCGCCGCGACCGGTGAAAGTGACGCCATCGGCCAAGGCCAGGt                                               <  5:237005/148‑1 (MQ=255)                                                                                                        gCCTGGGCCGGCGACAGGGTGAGGCTGAACAGGCCGTCGGCACCGACGACGCCGGTGCCAATTAGGTTGCCGGCAGCATCGCGCACTTGCACGGTAGCGCCGGGCTCGCCGCGACCGGTGAAAGTGACGCCATCGGCCAAGGCCAGGt                                               <  3:181242/148‑1 (MQ=255)                                                                                                        gCCTGGGCCGGCGACAGGGTGAGGCTGAACAGGCCGTCGGCACCGACGACGCCGGTGCCAATTAGGTTGCCGGCAGCATCGCGCACTTGCACGGTAGCGCCGGGCTCGCCGCGACCGGTGAAAGTGACGCCATCGGCCAAGGCCAGGt                                               >  4:319822/1‑148 (MQ=255)                                                                                                          cTGGGCCGGCGACAGGGTGAGGCTGAACAGGCCGTCGGCACCGACGACGCCGGTGCCAATTAGGTTGCCGGCAGCATCGCGCACTTGCACGGTAGCGCCGGGCTCGCCGCGACCGGTGAAAGTGACGCCATCGGCCAAGGCCAGGTCAg                                            >  3:290111/1‑149 (MQ=255)                                                                                                               ccGGCGACAGGGTGAGGCTGAACAGGCCGTCGGCACCGACGACGCCGGTGCCAATTAGGTTGCCGGCAGCATCGCGCACTTGCACGGTAGCGCCGGGCTCGCCGCGACCGGTGAAAGTGACGCCATCGGCCAAGGCCAGGTCAGTCGGt                                       >  6:189456/1‑149 (MQ=255)                                                                                                                cGGCGACAGGGTGAGGCTGAACAGGCCGTCGGCACCGACGACGCCGGTGCCAATTAGGTTGCCGGCAGCATCGCGCACTTGCACGGTAGCGCCGGGCTCGCCGCGACCGGTGAAAGTGACGCCATCGGCCaa                                                       <  5:348709/132‑1 (MQ=255)                                                                                                                cGGCGACAGGGTGAGGCTGAACAGGCCGTCGGCACCGACGACGCCGGTGCCAATTAGGTTGCCGGCAGCATCGCGCACTTGCACGGTAGCGCCGGGCTCGCCGCGACCGGTGAAAGTGACGCCATCGGCCaa                                                       >  6:348709/1‑132 (MQ=255)                                                                                                                cGGCGACAGGGTGAGGCTGAACAGGCCGTCGGCACCGACGACGCCGGTGCCAATTAGGTTGCCGGCAGCATCGCGCACTTGCACGGTAGCGCCGGGCTCGCCGCGACCGGTGAAAGTGACGCCATCGGCCAAGGCCAGGTCAGTCGGtt                                      <  2:288630/149‑1 (MQ=255)                                                                                                                 ggCGACAGGGTGAGGCTGAACAGGCCGTCGGCACCGACGACGCCGGTGCCAATTAGGTTGCCGGCAGCATCGCGCACTTGCACGGTAGCGCCGGGCTCGCCGCGACCGGTGAAAGTGACGCCATCGGCCAAGGCCAGGTCAGTCGGTtg                                     <  3:319822/149‑1 (MQ=255)                                                                                                                  gCGACAGGGTGAGGCTGAACAGGCCGTCGGCACCGACGACGCCGGTGCCAATTAGGTTGCCGGCAGCATCGCGCACTTGCACGGTAGCGCCGGGCTCGCCGCGACCGGTGAAAGTGACGCCATCGGCCAAGGCCAGGTCAGTCGGTtgt                                    <  8:150827/149‑1 (MQ=255)                                                                                                                    gACAGGGTGAGGCTGAACAGGCCGTCGGCACCGACGACGCCGGTGCCAATTAGGTTGCCGGCAGCATCGCGCACTTGCACGGTAGCGCCGGGCTCGCCGCGACCGGTGAAAGTGACGCCATCGGCCAAGGCCAGGTCAGTCGGTTGTGc                                  <  2:216490/149‑1 (MQ=255)                                                                                                                        gggTGAGGCTGAACAGGCCGTCGGCACCGACGACGCCGGTGCCAATTAGGTTGCCGGCAGCATCGCGCACTTGCACGGTAGCGCCGGGCTCGCCGCGACCGGTGAAAGTGACGCCATCGGCCAAGGCCAGGTCAGTCGGTTGTGCAggc                              <  2:253822/149‑1 (MQ=255)                                                                                                                             aGGCTGAACAGGCCGTCGGCACCGACGACGCCGGTGGCAATTATGTTGCCGGCAGCATCGCGCACTTGCACGGTAGCGCCGGGCTCGCCGCGACCGGTGAAAGTGACGCCATCGGCCAAGGCCAGGTCAGTCGGTTGTGCAGGCGgca                          <  3:26017/148‑1 (MQ=255)                                                                                                                             aGGCTGAACAGGCCGTCGGCACCGACGACGCCGGTGCCAATTAGGTTGCCGGCAGCATCGCGCACTTGCACGGTAGCGCCGGGCTCGCCGCGACCGGTGAAAGTGACGCCATCGGCCAAGGCCAGGTCAGTCGGTTGTGCAGGCGgca                          <  5:260928/148‑1 (MQ=255)                                                                                                                                cTGAACAGGCCGTCGGCACCGACGACGCCGGTGCCAATTAGGTTGCCGGCAGCATCGCGCACTTGCACGGTAGCGCCGGGCTCGCCGCGACCGGTGAAAGTGACGCCATCGGCCAAg                                                      >  8:316617/1‑117 (MQ=255)                                                                                                                                cTGAACAGGCCGTCGGCACCGACGACGCCGGTGCCAATTAGGTTGCCGGCAGCATCGCGCACTTGCACGGTAGCGCCGGGCTCGCCGCGACCGGTGAAAGTGACGCCATCGGCCAAg                                                      <  7:316617/117‑1 (MQ=255)                                                                                                                                cTGAACAGGCCGTCGGCACCGACGACGCCGGTGCCAATTAGGTTGCCGGCAGCATCGCGCACTTGCACGGTAGCGCCGGGCTCGCCGCGACCGGTGAAAGTGACGCCATCGGCCAAGGCCAGGTCAGTCGGTTGTGCAGGCGGCAGCaa                      <  4:74278/149‑1 (MQ=255)                                                                                                                                 tGAACAGGCCGTCGGCACCGACGACGCCGGTGCCAATTAGGTTGCCGGCAGCATCGCGCACTTGCACGGTAGCGCCGGGCTCGCCGCGACCGGTGAAAGTGACGCCATCGGCCAAGGCCAGGTCAGTCGGTTGTGCAGGCGGCAGCAAg                     >  8:334023/1‑149 (MQ=255)                                                                                                                                   aaCAGGCCGTCGGCACCGACGACGCCGGTGCCAATTAGGTTGCCGGCAGCATCTCGCACTTGCACGGTAGCGCCGGGCTCGCCGCGACCGGTGAAAGTGACGCCATCGGCCAAGGCCAGGTCAGTCGGTTGTGCAGGCGGCAGCAAGt                    <  6:351403/148‑1 (MQ=255)                                                                                                                                   aaCAGGCCGTCGGCACCGACGACGCCGGTGCCAATTAGGTTGCCGGCAGCATCGCGCACTTGCACGGTAGCGCCGGGCTCGCCGCGACCGGTGAAAGTGACGCCATCGGCCAAGGCCAGGTCAGTCGGTTGTGCAGGCGGCATCAAGTc                   >  8:179126/1‑149 (MQ=255)                                                                                                                                    aCAGGCCGTCGGCACCGACGACGCCGGTGCCAATTAGGTTGCCGGCAGCATCGCGCACTTGCACGGTAGCGCCGGGCTCGCCGCGACCGGTGAAAGTGACGCCATCGGCCAAGGCCAGGTCAGTCGGTTGTGCAGGCGGCAGCAAGTCg                  <  5:189456/149‑1 (MQ=255)                                                                                                                                     cAGGCCGTCGGCACCGACGACGCCGGTGCCAATTAGGTTGCCGGCAGCATCGCGCACTTGCACGGTAGCGCCGGGCTCGCCGCGACCGGTGAAAGTGACGCCATCGGCCAAGGCCAGGTCAGTCGGTTGTGCAGGCGGCAGCAAGTCgg                 >  1:218428/1‑149 (MQ=255)                                                                                                                                       cctaattggCACCGGCGTCGTCGGTGCCAATTAGGTTGCCGGCAGCATCGCGCACTTGCACGGTAGCGCCGGGCTCGCCGCGACCGGTGAAAGTGACGCCATCGGCCAAGGCCAGGTCAGTCGGTTGTGCAGGCGGCAGCAAGTCGGcc               >  6:14506/8‑149 (MQ=21)                                                                                                                                        gCCGTCGGCACCGACGACGCCGGTGCCAATTAGGTTGCCGGCAGCATCGCGCACTTGCACGGTAGCGCCGGGCTCGCCGCGACCGGTGAAAGTGACGCCATCGGCCAAGGCCAGGTCAGTCGGTTGTGCAGGCGGCAGCAAGTCGGCCg              <  4:6900/149‑1 (MQ=255)                                                                                                                                          cGTCGGCACCGACGACGCCGGTGCCAATTAGGTTGCCGGCAGCATCGCGCACTTGCACGGTAGCGCCGGGCTCGCCGCGACCGGTGAAAGTGACGCCATCGGCCAAGGCCAGGTCAGTCGGTTGTGCAGGCGGCAGCAAGTCGGCCGAg            <  3:37521/149‑1 (MQ=255)                                                                                                                                               gCACCGGCGTCGTCGGTGCCAATTAGGTTGCCGGCAGCATCGCGCACTTGCACGGTAGCGCCGGGCTCGCCGCGACCGGTGAAAGTGACGCCATCGGCCAAGGCCAGGTCAGTCGGTTGTGCAGGCGGCAGCAAGTCGGCCGAGGTGAc       >  5:288099/1‑149 (MQ=255)                                                                                                                                                cACCGACGACGCCGGTGCCAATTAGGTTGCCGGCAGCATCGCGCACTTGCACGGTAGCGCCGGGCTCGCCGCGACCg                                                                              <  8:108919/77‑1 (MQ=255)                                                                                                                                                cACCGACGACGCCGGTGCCAATTAGGTTGCCGGCAGCATCGCGCACTTGCACGGTAGCGCCGGGCTCGCCGCGACCg                                                                              >  7:108919/1‑77 (MQ=255)                                                                                                                                                  ccGACGACGCCGGTGCCAATTAGGTTGCCGGCAGCATCGCGCACTTGCACGGTAGCGCCGGGCTCGCCGCGACCGGTGAAAGTGACGCCATCGGCCAAGGCCAGGTCAGTCGGTTGTGCAGGCGGCAGCAAGTCGGCCGAGGTGACCGa    <  2:218428/149‑1 (MQ=255)                                                                                                                                                   cgacgaCGCCGGTGCCAATTAGGTTGCCGGCAGCATCGCGCACTTGCACGGTAGCGCCGGGCTCGCCGCGACCGGTGAAAGTGACGCCATCGGCCAAGGCCAGGTCAGTCGGTTGTGCAGGCGGCAGCAAGTCGGCCGAGGTGACCGAg   >  4:171611/1‑149 (MQ=255)                                                                                                                                                    gacgacGCCGGTGCCAATTAGGTTGCCGGCAGCATCGCGCACTTGCACGGTAGCGCCGGGCTCGCCGCGACCGGTGAAAGTGACGCCATCGGCCAAGGCCAGGTCAGTCGGTTGTGCAGGCGGCAGCAAGTCGGCCGAGGTGACCGAGc  >  2:95571/1‑149 (MQ=255)                                                                                                                                                    gacgacGCCGGTGCCAATTAGGTTGCCGGCAGCATCGCGCACTTGCACGGTAGCGCCGGGCTCGCCGCGACCGGTGAAAGTGACGCCATCGGCCAAGGCCAGGTCAGTCGGTTGTGCAGGCGGCAGCAAGTCGGCCGAGGTGACCGAGc  >  3:21973/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                    GGTGACCGCCTCGGGCGGTGTGATATCCGGGGCTGTGAACTCCAGCGGTATCGAACTGTTGCCGGCGGCATCCACCAGACGCACGTCGAGTGCTTCACCATTGGCCTGGGCCGGCGACAGGGTGAGGCTGAACAGGCCGTCGGCACCGACGACGCCGGTGCCAATTAGGTTGCCGGCAGCATCGCGCACTTGCACGGTAGCGCCGGGCTCGCCGCGACCGGTGAAAGTGACGCCATCGGCCAAGGCCAGGTCAGTCGGTTGTGCAGGCGGCAGCAAGTCGGCCGAGGTGACCGAGC  >  NC_002947/941785‑942080

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑