Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,038,237 T→G 6.9% V386V (GTA→GTC)  PP_0897 ← class 1 fumarate hydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,038,2370TG 6.9% 275.5 / 9.5 87V386V (GTA→GTC) PP_0897class 1 fumarate hydratase
Reads supporting (aligned to +/- strand):  ref base T (41/40);  new base G (3/3);  total (44/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.16e-01

CAGGTACACGGCCTTGTTGTCCTTGATCGCCTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCC‑‑‑‑‑AGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAACCATGCGCTTGTGCGCGGCATCACGGCCGGTCAGCATCTTGCCGTTGAG  >  NC_002947/1038090‑1038383
                                                                                                                                                        |                                                                                                                                                  
cAGGTACACGGCCTTGTTGTCCTTGATCGCCTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCC‑‑‑‑‑AGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTa                                                                                                                                                   >  6:168833/1‑149 (MQ=255)
 aGGTACACGGCCTTGTTGTCCTTGATCGCCTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCC‑‑‑‑‑AGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTa                                                                                                                                                   <  6:251726/148‑1 (MQ=255)
  ggTACACGGCCTTGTTGTCCTTGATCGCCTCGATGGCGGTCGGGCCACGCTCGGACTTCCAAATCATGCCCAGCAGGCG‑‑‑‑‑AGTCTGCTCAAGGATCTGGCGAGTGAACATGTACATTCGGGAAGCAGTGGTCGGGCCGGCTGGGCCTAc                                                                                                                                                  >  8:46390/1‑148 (MQ=255)
      cacGGCCTTGTTGTCCTTGATCGCCTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCC‑‑‑‑‑AGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTAGTCCCAGCCGGCCCGACCACt                                                                                                                                              <  8:146816/148‑1 (MQ=255)
      cacGGCCTTGTTGTCCTTGATCGCCTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCC‑‑‑‑‑AGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTAGGCCCAGCCGGCCCGACCACt                                                                                                                                              <  2:178732/148‑1 (MQ=255)
       acGGCCTTGTTGTCCTTGATCGCCTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCC‑‑‑‑‑AGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTc                                                                                                                                            >  4:279676/1‑149 (MQ=255)
       acGGCCTTGTTGTCCTTGATCGCCTCGATAGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCC‑‑‑‑‑AGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTc                                                                                                                                            >  1:21156/1‑149 (MQ=255)
        cGGCCTTGTTGTCCTTGATCGCCTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCC‑‑‑‑‑AGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTc                                                                                                                                            >  8:259411/1‑148 (MQ=255)
        cGGCCTTGTTGTCCTTGATCGCCTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCC‑‑‑‑‑AGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTc                                                                                                                                            <  7:259411/148‑1 (MQ=255)
        cGGCCTTGTTGTCCTTGATCGCCTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCC‑‑‑‑‑AGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCg                                                                                                                                           >  5:360918/1‑149 (MQ=255)
         ggCCTTGTTGTCCTTGATCGCCTCGATAGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCC‑‑‑‑‑AGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGt                                                                                                                                          <  2:21156/149‑1 (MQ=255)
            cTTGTTGTCCTTGATCGCCTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCC‑‑‑‑‑AGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTAGGCCCAGCCGGCCCGACCACtgctaccc                                                                                                                                       <  7:69975/149‑8 (MQ=38)
                 tgtTCTTTATCGCCTCGATGGCGGTGGGGCCAAGCTCGGACTTGTCAATAATTCCTTCCTTTTCTTTTTTTTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTca                                                                                                                                        <  1:311262/148‑1 (MQ=255)
                  gtCCTTGATCGCCTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCC‑‑‑‑‑AGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCg                                                                                                                                 >  5:155996/1‑149 (MQ=255)
                   tCCTTGATCGCCTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCC‑‑‑‑‑AGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCgg                                                                                                                                >  1:9252/1‑149 (MQ=255)
                    ccTTGATCGCCTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCC‑‑‑‑‑AGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCggg                                                                                                                               >  8:42124/1‑149 (MQ=255)
                      ttGATCGCCTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGAC‑‑‑‑‑AGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTc                                                                                                                             >  3:307181/1‑149 (MQ=255)
                        gATCGCCTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCC‑‑‑‑‑AGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTc                                                                                                                             <  5:214924/147‑1 (MQ=255)
                          tCGCCTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCC‑‑‑‑‑AGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGAcc                                                                                                                         >  1:37625/1‑149 (MQ=255)
                             ccTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCC‑‑‑‑‑AGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCggg                                                                                                                      >  5:111557/1‑149 (MQ=255)
                             ccTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCC‑‑‑‑‑AGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCggg                                                                                                                      >  3:2456/1‑149 (MQ=255)
                             ccTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCC‑‑‑‑‑AGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCAACCACCGGGTCGACCggg                                                                                                                      >  3:39956/1‑149 (MQ=255)
                              cTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCC‑‑‑‑‑AGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGc                                                                                                                     >  4:312417/1‑149 (MQ=255)
                               tCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCC‑‑‑‑‑AGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGcc                                                                                                                    >  4:206685/1‑149 (MQ=255)
                                     gCGGTCGGGCCACGCTCGGAGTTGCCAATCATGCCCAGCAGGCC‑‑‑‑‑AGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCTTACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACgta                                                                                                              <  4:296207/149‑1 (MQ=255)
                                      cGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCC‑‑‑‑‑AGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACgtag                                                                                                             <  6:270965/149‑1 (MQ=255)
                                        gTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCC‑‑‑‑‑AGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACgtagta                                                                                                           <  7:324441/149‑1 (MQ=255)
                                             gCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCC‑‑‑‑‑AGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATg                                                                                                       >  2:238521/1‑148 (MQ=255)
                                                  gCTCGGACTTGCCAATCATGCCCAGCAGGCC‑‑‑‑‑AGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCgg                                                                                                 <  5:168833/149‑1 (MQ=255)
                                                       gACTTGCCAATCATTCCCAGCAGGCC‑‑‑‑‑AGTCTGCTCAAGGAGCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCttt                                                                                            <  3:351428/149‑1 (MQ=255)
                                                          ttGCCAATCATGCCCAGCAGGCC‑‑‑‑‑AGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAg                                                                                         >  4:80534/1‑149 (MQ=255)
                                                            gCCAATCATGCCCAGCAGGCC‑‑‑‑‑AGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAgg                                                                                        >  8:135716/1‑148 (MQ=255)
                                                            gCCAATCATGCCCAGCAGGCC‑‑‑‑‑AGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAgg                                                                                        <  8:270722/148‑1 (MQ=255)
                                                            gCCAATCATGCCCAGCAGGCC‑‑‑‑‑AGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAg                                                                                         <  5:139707/147‑1 (MQ=255)
                                                            gCCAATCATGCCCAGCAGGCC‑‑‑‑‑AGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAg                                                                                         <  3:279676/147‑1 (MQ=255)
                                                               aaTCATGCCCAGCAGGCC‑‑‑‑‑AGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTa                                                                                    <  2:9252/149‑1 (MQ=255)
                                                                aTCATGCCCAGCAGGCC‑‑‑‑‑AGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGaa                                                                                                     <  6:102420/131‑1 (MQ=255)
                                                                aTCATGCCCAGCAGGCC‑‑‑‑‑AGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGaa                                                                                                     >  5:102420/1‑131 (MQ=255)
                                                                aTCATGCCCAGCAGGCC‑‑‑‑‑AGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTAc                                                                                   <  6:208675/149‑1 (MQ=255)
                                                                   aTGCCCAGCAGGCC‑‑‑‑‑AGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCg                                                                                 <  3:80534/148‑1 (MQ=255)
                                                                       ccAGCAGGCC‑‑‑‑‑AGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCGTACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGc                                                                                                                     <  6:209580/108‑1 (MQ=255)
                                                                       ccAGCAGGCC‑‑‑‑‑AGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCGTACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGc                                                                                                                     >  5:209580/1‑108 (MQ=255)
                                                                       ccAGCAGGCC‑‑‑‑‑AGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCaa                                                                             <  7:135716/148‑1 (MQ=255)
                                                                            agGCC‑‑‑‑‑AGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTc                                                                       <  6:179089/149‑1 (MQ=255)
                                                                            agGCC‑‑‑‑‑AGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTc                                                                       <  6:360918/149‑1 (MQ=255)
                                                                            agGCC‑‑‑‑‑AGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTc                                                                       <  3:312417/149‑1 (MQ=255)
                                                                             ggCC‑‑‑‑‑AGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCg                                                                      <  7:42124/149‑1 (MQ=255)
                                                                             ggCC‑‑‑‑‑AGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCg                                                                      <  6:132487/149‑1 (MQ=255)
                                                                                c‑‑‑‑‑AGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTc                                                                                                                                         >  7:6104/1‑79 (MQ=255)
                                                                                c‑‑‑‑‑AGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTc                                                                                                                                         <  8:6104/79‑1 (MQ=255)
                                                                                          tGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGtt                                                              >  5:25256/1‑149 (MQ=255)
                                                                                            cTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGtt                                                              <  6:111557/147‑1 (MQ=255)
                                                                                            cTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGa                                                            <  2:37625/149‑1 (MQ=255)
                                                                                             tCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAg                                                                                         <  2:349613/119‑1 (MQ=255)
                                                                                             tCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAg                                                                                         >  1:349613/1‑119 (MQ=255)
                                                                                             tCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGTCAACTCTTCGCCACGGTTGAg                                                           >  8:253040/1‑149 (MQ=255)
                                                                                                  gATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAg                                                                                         <  4:196124/114‑1 (MQ=255)
                                                                                                  gATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAg                                                                                         >  3:196124/1‑114 (MQ=255)
                                                                                                  gATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCAtt                                                       >  1:219482/1‑148 (MQ=255)
                                                                                                      tGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAAc                                                  >  8:33576/1‑149 (MQ=255)
                                                                                                       ggCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAAc                                                  >  5:249493/1‑148 (MQ=255)
                                                                                                        gCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCa                                                    <  4:147174/145‑1 (MQ=255)
                                                                                                          gAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAACCATg                                              >  2:77166/1‑149 (MQ=255)
                                                                                                          gAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAACCATg                                              >  3:154618/1‑149 (MQ=255)
                                                                                                          gAGTGAACTTGTCCATACGGGTAGCAGTCGACGGGACGGCTGGGCATACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTACTAGATGAAGCCGCCTTTCAGCTCTACCGGCAACTCTTCGCCACGGATGAGCATTTCAACCATg                                              >  5:299918/1‑149 (MQ=255)
                                                                                                              gAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAACCATGCGCt                                          >  2:271922/1‑149 (MQ=255)
                                                                                                               aaCTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAACCATGCGCtt                                         <  1:238521/149‑1 (MQ=255)
                                                                                                                 cTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAACCATGCGCTtgt                                       <  6:155996/149‑1 (MQ=255)
                                                                                                                  ttGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTAcc                                                                                  >  8:130525/1‑105 (MQ=255)
                                                                                                                  ttGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTAcc                                                                                  <  7:130525/105‑1 (MQ=255)
                                                                                                                  ttGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAACCATGCGCTtgtg                                      >  1:130001/1‑149 (MQ=255)
                                                                                                                     tCCATAAGTGTAGCAGTGGTCGGTCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAACCATGCGCTTGTgcg                                    <  7:319541/148‑1 (MQ=255)
                                                                                                                       cATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAACCATGCGCTTGTGcgcg                                  <  1:271922/148‑1 (MQ=255)
                                                                                                                             tgacgaaGTGGTAGGCCCAGCCGGCCCGACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAACCATGCGCTTGTGCGCGGCATCAc                           >  8:42703/7‑149 (MQ=255)
                                                                                                                             tgacgaaGTGGTAGGCCCAGCCGGCCCGACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAACCATGCGCTTGTGCGCGGCATCAc                           >  7:214499/7‑149 (MQ=255)
                                                                                                                              gTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAACCATGCGCTTGTGCGCGGCATCACg                          <  4:2456/149‑1 (MQ=255)
                                                                                                                                  cAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAACCATGCGCTTGTGCGCGGCATCACGGCCg                      <  4:154618/149‑1 (MQ=255)
                                                                                                                                  aaGTGGTAGGCCCAGCCGGCCCGACCCCTTCGTCCCCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGGTGAGCATTTCAACCATGCGCTTGTGCGCGGCTTCACGGCCg                      >  7:247704/2‑149 (MQ=255)
                                                                                                                                     tGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAACCATGCGCTTGTGCGCGGCATCACGGCCggac                   >  4:360115/1‑147 (MQ=255)
                                                                                                                                       gTCGGGCCTGCTGTGACTACCACTTCGTCACCCACCGGGTCGACCGGTCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAACCATGCGCTTGTGCGCGGCATCACGGCCGGTCa                  <  4:39956/148‑1 (MQ=255)
                                                                                                                                       gTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAACCATGCGCTTGTGCGCGGCATCACGGCCg                      <  1:29160/144‑1 (MQ=255)
                                                                                                                                       gTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAACCATGCGCTTGTGCGCGGCATCACGGCCg                      >  2:29160/1‑144 (MQ=255)
                                                                                                                                            gCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAACCATGCGCTTGTGCGCGGCATCACGGCCGGTCAGCATCt            >  2:84776/1‑149 (MQ=255)
                                                                                                                                                  tGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAACCATGCGCTTGTGCGCGGCATCACGGCCGGTCAGCATCTTGCCGt      >  1:226913/1‑149 (MQ=255)
                                                                                                                                                       cTACCACTTCGTCCCCCTCCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACGGGCAACTCTTCGCGACGGTTGAGCATTTCAACCATGCGCTTGTGCGCGGCATCACGGCCGGTCAGc                <  7:279844/134‑1 (MQ=255)
                                                                                                                                                       cTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAACCATGCGCTTGTGCGCGGCATCACGGCCGGTCAGCATCTTGCCGTTGAg  >  7:17610/1‑148 (MQ=255)
                                                                                                                                                       cTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAACCATGCGCTTGTGATCTGCATCACGGCCGGTCAGc                >  8:279844/1‑134 (MQ=255)
                                                                                                                                                        |                                                                                                                                                  
CAGGTACACGGCCTTGTTGTCCTTGATCGCCTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCC‑‑‑‑‑AGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAACCATGCGCTTGTGCGCGGCATCACGGCCGGTCAGCATCTTGCCGTTGAG  >  NC_002947/1038090‑1038383

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑