Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,051,971	C→T	8.2%	A466T (GCT→ACT) ‡	PP_0910 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,051,971	0	C	T	8.2%	244.2 / 9.1	86	A466T (GCT→ACT) ‡	PP_0910	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (39/39);  new base T (5/2);  total (45/41)
Fisher's exact test for biased strand distribution p-value = 4.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.11e-01

CGGCGGAGGTCACTGCTTGCGGGTCTTGTGGCGAGCAGGCACCGAGGGCGAGTGCGGCGAGGCTGGTGAACAACAGTTTGGGTCGGAACATGCCCGGCTCCTTTGCGCGTTTAAAGTGAGTTCAGGAACGCGAGCAACGCAGCGCGCTGCTCGGCATCGAAAGTGAGTACGAAATTGCGTGCCGCC‑TCGGCTTCGCCACCGTGCCACAGCACGGCCTCAAGCAGGTTGCGGGCGCGGCCGTCATGCAGGAACTGGCTATGGCCACTGACCGTTTCGCTCAGGCCGATGC  >  NC_002947/1051829‑1052117                                                                                                                                               |                                                                                                                                                    cggcggAGGTCACTGCTTGCGGGTCTTGTGGCGAGCAGGCACCGAGGGCGAGTGCGGCGAGGCTGGTGAACAACAGTTTGGGTCGGAACATGCCCGGCTCCTTTGCGCGTTTAAAGTGAGTTCAGGAACGCGAGCAACGCAGCGCgctg                                                                                                                                               >  6:17711/1‑149 (MQ=255)    cggAGGTCACTGCTTGCGGGTCTTGTGGCGAGCAGGCACCGAGGGCGAGTGCGGCGAGGCTGGTGAACAACAGTTTGGGTCGGAACATGCCCGGCTCCTTTGCGCGTTTAAAGTGAGTTCAGGAACGCGAGCAACGCAGCGCGCTGCTc                                                                                                                                            <  7:39374/149‑1 (MQ=255)     ggAGGTCACTGCTTGCGGGTCTTGTGGCGAGCAGGCACCGAGGGCGAGTGCGGCGAGGCTGGTGAACAACAGTTTGGGTCGGAACATGCCCGGCTCCTTTGCGCGTTTAAAGTGAGTTCAGGAACGCGAGCAACGCAGCGCGCTGCTCg                                                                                                                                           <  6:57233/149‑1 (MQ=255)         gTCACTGCTTGCGGGTCTTGTGGCGAGCAGGCACCGAGGGCGAGTGCGGCGAGGCTGGTGAACAACAGTTTGGGTCGGAACATGCCCGGCTTCTTTGCGCGTTTAAAGTGAGTTCAGGAACGCGAGCAACGCAGCGCGCTGCTCGGCAt                                                                                                                                       <  5:322011/149‑1 (MQ=255)         gTCACTGCTTGCGGGTCTTGTGGCGAGCAGGCACCGAGGGCGAGTGCGGCGAGGCTGGTGAACAACAGTTTGGGTCGGAACATGCCCGGCTCCTTTGCGCGTTTAAAGTGAGTTCAGGAACGCGAGCAGCGCGCTGCGTTGCTCgcgtt                                                                                                                                       >  7:169246/1‑145 (MQ=255)         gTCACTGCTTGCGGGTCTTGTGGCGAGCAGGCACCGAGGGCGAGTGCGGCGAGGCTGGTGAACAACAGTTTGGGTCGGAACATGCCCGGCTCCTTTGCGCGTTTAAAGTGAGTTCAGGAACGCGAGCAGCGCGCTGCGTTGCTCgcgtt                                                                                                                                       >  7:96832/1‑145 (MQ=255)         gTCACTGCTTGCGGGTCTTGTGGCGAGCAGGCACCGAGGGCGAGTGCGGCGAGGCTGGTGAACAACAGTTTGGGTCGGAACATGCCCGGCTCCTTTGCGCGTTTAAAGTGAGTTCAGGAACGCGAGCAACGCAGCGCGCTGCTCGGCAt                                                                                                                                       <  4:189794/149‑1 (MQ=255)         gTCACTGCTTGCGGGTCTTGTGGCGAGCAGGCACCGAGGGCGAGTGCGGCGAGGCTGGTGAACAACAGTTTGGGTCGGAACATGCCCGGCTCCTTTGCGCGTTTAAAGTGAGTTCAGGAACGCGAGCAACGCAGCGCGCTGCTCGGCAt                                                                                                                                       <  4:96230/149‑1 (MQ=255)           cACTGCTTGCGGGTCTTGTGGCGAGCAGGCACCGAGGGCGAGTGCGGCGAGGCTGGTGAACAACAGTTTGGGTCGGAACATGCCCGGCTCCTTTGCGCGTTTAAAGTGAGTTCAGGAACGCGAGCAACGCAGCGCGCTGCTCGGCATc                                                                                                                                      >  3:370405/1‑148 (MQ=255)            aCTGCTTGCGGGTCTTGTGGCGAGCAGGCACCGAGGGCGAGTGCGGCGAGGCTGGTGAACAACAGTTTGGGTCGGAACATGCCCGGCTCCTTTGCGCGTTTAAAGTGAGTTCAGGAACGCGAGCAGCGCGCTGCGTTGCTCgcgttcc                                                                                                                                     <  8:169246/148‑7 (MQ=255)            aCTGCTTGCGGGTCTTGTGGCGAGCAGGCACCGAGGGCGAGTGCGGCGAGGCTGGTGAACAACAGTTTGGGTCGGAACATGCCCGGCTCCTTTGCGCGTTTAAAGTGAGTTCAGGAACGCGAGCAGCGCGCTGCGTTGCTCgcgttcc                                                                                                                                     <  8:96832/148‑7 (MQ=255)                cTTGCGGTTCTTGTGGAGAGCAGTCACCGAGGGCGAGTGCGGCGAGGCTGGTGAACAACAGTTTGGGTCGGAACATGCCCGGCTCCTTTTCGCGTTTAAAGTGAGTTCAGGAACGCGAGCAACGCAGCGCGCTTCTCGGCATCGAAAGt                                                                                                                                <  8:161837/149‑1 (MQ=255)                 ttGCGGGTCTTGTGGCGAGCAGGCACCGAGGGCGAGTGCGGCGAGGCTGGTGAACAACAGTTTGGGTCGGAACATGCCCGGCTCCTTTGCGCGTTTAAAGTGAGTTCAGGAACGCGAGCAACGCAGCGCGCTGCTCGGCATCGAAAGt                                                                                                                                >  7:124433/1‑148 (MQ=255)                 ttGCGGGTCTTGTGGCGAGCAGGCACCGAGGGCGAGTGCGGCGAGGCTGGTGAACAACAGTTTGGGTCGGAACATGCCCGGCTCCTTTGCGCGTTTAAAGTGAGTTCAGGAACGCGAGCAACGCAGCGCGCTGCTCGGCATCGAAAGt                                                                                                                                >  3:130679/1‑148 (MQ=255)                 ttGCGGGTCTTGTGGCGAGCAGGCACCGAGGGCGAGTGCGGCGAGGCTGGTGAACAACAGTTTGGGTCGGAACATGCCCGGCTCCTTTGCGCGTTTAAAGTGAGTTCAGGAACGCGAGCAACGCAGCGCGCTGCTCGGCATCGAAAGTg                                                                                                                               >  1:146107/1‑149 (MQ=255)                   gCGGGTCTTGTGGCGAGCAGGCACCGAGGGCGAGTGCGGCGAGGCTGGTGAACAACAGTTTGGGTCGGAACATGCCCGGCTCCTTTGCGCGTTTAAAGTGAGTTCAGGAACGCGAGCAACGCAGCGCGCTGCTCGGCATCGAAAGTGa                                                                                                                              <  6:62491/148‑1 (MQ=255)                      ggTCTTGTGGCTAGCAGGCACCGAGGGCGAGTGCGGCGAGGCTGGTGAACAACAGTTTGGGTCGGAACATGCCCGGCTCCTTTGCGCGTTTAAAGTGAGTTCAGGAACGCGAGCAACGCAGCGCGCTGCTCGGCATCGAAAGTGAGTAc                                                                                                                          <  7:256297/149‑1 (MQ=255)                             tgGCGAGCAGGCACCGAGGGCGAGTGCGGCGAGGCTGGTGAACAACAGTTTGGGTCGGAACATGCCCGGCTCCTTTGCGCGTTTAAAGTGAGTTCAGGAACGCGAGCAACGCAGCGCGCTGCTCGGCATCGAAAGTGAGTACGAAATTg                                                                                                                   >  4:45453/1‑149 (MQ=255)                             tgGCGAGCAGGCACCGAGGGCGAGTGCGGCGAGGCTGGTGAACAACAGTTTGGGTCGGAACATGCCCGGCTCCTTTGCGCGTTTAAAGTGAGTTCAGGAACGCGAGCAACGCAGCGCGCTGCTCGGCATCGAAAGTAAGTACGAAATTg                                                                                                                   >  8:238341/1‑149 (MQ=255)                               gCGAGCAGGCACCGAGGGCGAGTGCGGCGAGGCTGGTGAACAACAGTTTGGGTCGGAACATGCCCGGCTCCTTTGCGCGTTTAAAGTGAGTTCAGGAACGCGAGCAACGCAGCGCGCTGCTCGGCATCGAAAGTGAGTACGAAATTGCg                                                                                                                 >  4:144714/1‑149 (MQ=255)                               gCGAGCAGGCACCGAGGGCGAGTGCGGCGAGGCTGGTGAACAACAGTTTGGGTCGGAACATGCCCGGCTCCTTTGCGCGTTTAAAGTGAGTTCAGGAACGCGAGCAACGCAGCGCGCTGCTCGGCATCGAAAGTGAGTACGAAATTGCg                                                                                                                 >  4:150489/1‑149 (MQ=255)                               gCGAGCAGGCACCGAGGGCGAGTGCGGCGAGGCTGGTGAACAACAGTTTGGGTCGGAACATGCCCGGCTCCTTTGCGCGTTTAAAGTGAGTTCAGGAACGCGAGCAACGCAGCGCGCTGCTCGGCATCGAAAGTGAGTACGAAATTGCg                                                                                                                 >  4:356655/1‑149 (MQ=255)                                 gAGCAGGCACCGAGGGCGAGTGCGGCGAGGCTGGTGAACAACAGTTTGGGTCGGAACATGCCCGGCTCCTTTGCGCGTTTAAAGTGAGTTCAGGAACGCGAGCAACGCAGCGCGCTGCTCGGCATCGAAAGTGAGTACGAAATTGCGTg                                                                                                               >  2:214865/1‑149 (MQ=255)                                    cAGGCACCGAGGGCGAGTGCGGCGAGGCTGGTGAACAACAGTTTGGGTCGGAACATGCCCGGCTCCTTTGCGCGTTTAAAGTGAGTTCAGGAACGCGAGCAACGCAGCGCGCTGCTCGGCATCGAAAGTGAGt                                                                                                                            >  4:198836/1‑133 (MQ=255)                                    cAGGCACCGAGGGCGAGTGCGGCGAGGCTGGTGAACAACAGTTTGGGTCGGAACATGCCCGGCTCCTTTGCGCGTTTAAAGTGAGTTCAGGAACGCGAGCAACGCAGCGCGCTGCTCGGCATCGAAAGTGAGt                                                                                                                            <  3:198836/133‑1 (MQ=255)                                       gCACCGAGGGCGAGTGCGGCGAGGCTGGTGAACAACAGTTTGGGTCGGAACATGCCCGGCTCCTTTGCGCGTTTAAAGTGAGTTCAGGAACGCGAGCAACGCAGCGCGCTGCTCGGCATCGAAAGTGAGTAc                                                                                                                          <  2:379898/132‑1 (MQ=255)                                       gCACCGAGGGCGAGTGCGGCGAGGCTGGTGAACAACAGTTTGGGTCGGAACATGCCCGGCTCCTTTGCGCGTTTAAAGTGAGTTCAGGAACGCGAGCAACGCAGCGCGCTGCTCGGCATCGAAAGTGAGTAc                                                                                                                          >  1:379898/1‑132 (MQ=255)                                       gCACCGAGGGCGAGTGCGGCGAGGCTGGTGAACAACAGTTTGGGTCGGAACATGCCCGGCTCCTTTGCGCGTTTAAAGTGAGTTCAGGAACGCGAGCAACGCAGCGCGCTGCTCGGCATCGAAAGTGAGTACGAAATTGCGTGCCGCC‑t                                                                                                        >  1:378294/1‑149 (MQ=255)                                       gCACCGAGGGCGAGTGCGGCGAGGCTGGTGAACAACAGTTTGGGTCGGAACATGCCCGGCTCCTTTGCGCGTTTAAAGTGAGTTCAGGAACGCGAGCAACGCAGCGCGCTGCTCGGCATCGAAAGTGAGTACGAAATTGCGTGCCGCC‑t                                                                                                        >  3:349995/1‑149 (MQ=255)                                         aCCGAGGGCGAGTGCGGCGAGGCTGGTGAACAACAGTTTGGGTCGGAACATGCCCGGCTCCTTTGCGCGTTTAAAGTGAGTTCAGGAACGCGAGCAACGCAGCGCGCTGCTCGGCATCGAAAGTGAGTACGAAATTGCGTGCCGCC‑TCg                                                                                                      >  2:270465/1‑149 (MQ=255)                                         aCCGAGGGCGAGTGCGGCGAGGCAGGTGAACAACAGTTTGGGTCGGAACATGCCCGGCTCCTTTGCGCGTTTAAAGTGAGTTCAGGAACGCGAGCAACGCAGCGCGCTGCTCGGCATCGAAAGTGAGTACGAAATTGCGTGCCGCC‑TCg                                                                                                      >  3:2912/1‑149 (MQ=255)                                                 cGAGTGCGGCGAGGCTGGTGAACAACAGTTTGGGTCGGAACATGCCCGGCTCCTTTGCGCGTTTAAAGTGAGTTCAGGAACGCGAGCAACGCAGCGCGCTGCTCGGCATCGAAAGTGAGTACGAAATTGCGTGCCGCC‑TCGGCTTCGcc                                                                                              >  3:360052/1‑149 (MQ=255)                                                   actGCGGCGAGGCTGGTGAACAACAGTTTGGGTCGGAACATGCCCGGCTCCTTTGCGCGTTTAAAGTGAGTTCAGGAACGCGAGCAACGCAGCGCGCTCCTCGGCATCGAAAGTGAGTACGAAATTGCGTGCCGCC‑TCGGCTTCGCCAc                                                                                            >  4:312749/3‑149 (MQ=255)                                                     tGCGGCGAGGCTGGTGAACAACATTTTGGGTCGGAACATGCCCGGCTCCTTTGCGCGTTTAAAGTGAGTTCAGGAACGCGAGCAACGCAGCGCGCTGCTCGGCATCGAAAGTGAGTACGAAATTGCGTGCCGCC‑TCGGCTTCGCCACCg                                                                                          <  2:146107/149‑1 (MQ=255)                                                         gcgAGGCTGGTGAACAACAGTTTGGGTCGGAACATGCCCGGCTCCTTTGCGCGTTTAAAGTGAGTTCAGGAACGCGAGCAACGCAGCGCGCTGCTCGGCATCGAAAGTGAGTACGAAATTGCGTGCCGCC‑TCGGCTTCGCCACCGTGc                                                                                       >  8:106569/1‑148 (MQ=255)                                                                tGGTGAACAACAGTTTGGGTCGGAACATGCCCGGCTCCTTTGCGCGTTTAAAGTGAGTTCAGGAACGCGAGCAACGCAGCGCGCTGCTCGGCATCGAAAGTGa                                                                                                                              >  5:60547/1‑103 (MQ=255)                                                                tGGTGAACAACAGTTTGGGTCGGAACATGCCCGGCTCCTTTGCGCGTTTAAAGTGAGTTCAGGAACGCGAGCAACGCAGCGCGCTGCTCGGCATCGAAAGTGa                                                                                                                              <  6:60547/103‑1 (MQ=255)                                                                  gTGAACAACAGTTTGGGTCGGAACATGCCCGGCTCCTTTGCGCGTTTAAAGTGAGTTCAGGAACGCGAGCAACGCAGCGCGCTGCTCGGCATCGAAAGTGAGTACGAAATTGCGTGCCGCC‑TCGGCTTCGCCACCGTGCCACAGCACg                                                                              <  2:13347/148‑1 (MQ=255)                                                                  gTGAACAACAGTTTGGGTCGGAACATGCCCGGCTCCTTTGCGCGTTTAAAGTGAGTTCAGGAACGCGAGCAACGCAGCGCGCTGCTCGGCATCGAAAGTGAGTACGAAATTGCGTGCCGCC‑TCGGCTTCGCCACCGTGCCACAGCACg                                                                              <  1:270465/148‑1 (MQ=255)                                                                     aacaacAGTTTGGGTCGGAACATGCCCGGCTCCTTTGCGCGTTTAAAGTGAGTTCAGGAACGCGAGCAACGCAGCGCGCTGCTCGGCATCGAAAGTAAGTACGAAATTGCGTGCCGCC‑TCGGCTTCGCCACCGTGCCACAGCACGGcc                                                                           <  7:238341/148‑1 (MQ=255)                                                                       caacaGTTTGGGTCGGAACATGCCCGGCTCCTTTGCGCGTTTAAAGTGAGTTCAGGAACGCGAGCAACGCAGAGCGCTGCTCGGCATCGAAAGTCAGTACGAAATTGCGTGCCGCC‑TCGGCTTCGCCACCGTGCCACAGCACGGCCTc                                                                         >  2:249444/1‑148 (MQ=255)                                                                        aacaGTTTGGGTCGGAACATGCCCGGCTCCTTTGCGCGTTTAAAGTGAGTTCAGGAACGCGAGCAACGCAGCGCGCTGCTCGGCATCGAAAGTGAGTACGAAATTGCGTGCCGCC‑TCGGCTTCGCCACCGTGCCACAGCACGGCCTc                                                                         >  7:125953/1‑147 (MQ=255)                                                                        aacaGTTTGGGTCGGAACATGCCCGGCTCCTTTGCGCGTTTAAAGTGAGTTCAGGAACGCGAGCAACGCAGCGCGCTGCTCGGCATCGAAAGTGAGTACGAAATTGCGTGCCGCC‑TCGGCTTCGCCACCGTGCCACAGCACGGCCTc                                                                         >  5:133332/1‑147 (MQ=255)                                                                            gTTTGGGTCGGAACATGCCCGGCTCCTTTGCGCGTTTAAAGTGAGTTCAGGAACGCGAGCAACGCAGCGCGCTGCTCGGCATCGAAAGTGAGTACGAAATTGCGTGCCGCC‑TCGGCTTCGCCACCGTGTCACAGCACGGCCTCAAGCAg                                                                   <  3:45453/149‑1 (MQ=255)                                                                            gTTTGGGTCGGAACATGCCCGGCTCCTTTGCGCGTTTAAAGTGAGTTCAGGAACGCGAGCAACGCAGCGCGCTGCTCGGCATCGAAAGTGAGTACGAAATTGCGTGCCGCC‑TCGGCTTCGCCACCGTGCCACAGCACGGCCTCAAGCAg                                                                   <  7:106569/149‑1 (MQ=255)                                                                             tttGGGTCGGAACATGCCCGGCTCCTTTGCGCGTTTAAAGTGAGTTCAGGAACGCGAGCAACGCAGCGCGCTGCTCGGCATCGAAAGTGAGTACGAAATTGCGTGCCGCC‑TCGGCTTCGCCACCGTGCCACAGCACGGCCTCAAGCAgg                                                                  <  2:355038/149‑1 (MQ=255)                                                                                 ggTCGGAACATGCCCGGCTCCTTTGCGCGTTTAAAGTGAGTTCAGGAACGCGAGCAACGCAGCGCGCTGCTCGGCATCGAAAGTGAGTACGAAATTGCGTGCCGCC‑TCGGCTTCGCCACCGTGCCACAGCACGGCCTCAAGCAGGTTGc                                                              <  2:378294/149‑1 (MQ=255)                                                                                  gTCGGAACATGCCCGGCTCCTTTGCGCGTTTAAAGTGAGTTCAGGAACGCGAGCAACGCAGCGCGCTGCTCGGCATCGAAAGTGAGTACGAAATTGCGTGCCGCC‑TCGGCTTCGCCACCGTGCCACAGCACGGCCTCAAGCAGGTTGCg                                                             <  3:150489/149‑1 (MQ=255)                                                                                  gTCGGAACATGCCCGGCTCCTTTGCGCGTTTAAAGTGAGTTCAGGAACGCGAGCAACGCAGCGCGCTGCTCGGCATCGAAAGTGAGTACGAAATTGCGTGCCGCC‑TCGGCTTCGCCACCGTGCCACAGCACGGCCTCAAGCAGGTTGCg                                                             <  3:144714/149‑1 (MQ=255)                                                                                   tCGGAACATGCCCGGCTCCTTTGCGCGTTTAAAGTGAGTTCAGGAACGCGAGCAACGCAGCGCGCTGCTCGGCATCGAAAGTGAGTACGAAATTGCGTGCCGCC‑TCGGCTTCGCCACCGTGCCACAGCACGGCCTCAAGCAGGTTGCgg                                                            <  4:130679/149‑1 (MQ=255)                                                                                     ggAACATGCCCGGCTCCTTTGCGCGTTTAAAGTGAGTTCAGGAACGCGAGCAACGCAGCGCGCTGCTCGGCATCGAAAGTGAGTACGAAATTGCGTGCCGCC‑TCGGCTTCGCCACCGTGCCACAGCACGGCCTCAAGCAGGTTGCGGgc                                                          >  6:308799/1‑149 (MQ=255)                                                                                      gAACATGCCCGGCTCCTTTGCGCGTTTAAAGTGAGTTCAGGAACGCGAGCAACGCAGCGCGCTGCTCGGCATCGAAAGTGAGTACGAAATTGCGTGCCGCC‑TCGGCTTCGCCACCGTGCCACAGCACGGCCTCAAGCAGGTTGCGGgcg                                                         >  4:362499/1‑149 (MQ=255)                                                                                      gAACATGCCCGGCTCCTTTGCGCGTTTAAAGTGAGTTCAGGAACGCGAGCAACGCAGCGCGCTGCTCGGCATCGAAAGTGAGTACGAAATTGCGTGCCGCC‑TCGGCTTCGCCACCGTGCCACAGCACGGCCTCAAGCAGGTTGCGGgcg                                                         >  8:261294/1‑149 (MQ=255)                                                                                          aTGCCCGGCTCCTTTGCGCGTTTAAAGTGAGTTCAGGAACGCGAGCAACGCAGCGCGCTGCTCGGCATCGAAAGTGAGTACGAAATTGCGTGCCGCC‑TCGGCTTCGCCACCGTGCCACAGCACGGCCTCAAGCAGGTTGCGGGCGCGGc                                                     >  8:99506/1‑149 (MQ=255)                                                                                           tGCCCGGCTCCTTTGCGCGTTTAAAGTGAGTTCAGGAACGCGAGCAACGCAGCGCGCTGCTCGGCATCGAAAGTGAGTACGAAATTGCGTGCCGCC‑TCGGCTTCGCCACCGTGCCACAGCTCGGCCTCAAGCAGGTTGCGGGCGCGGcc                                                    <  1:214865/149‑1 (MQ=255)                                                                                           tGCCCGGCTCCTTTGCGCGTTTAAAGTGAGTTCAGGAACGCGAGCAACGCAGCGCGCTGCTCGGCATCGAAAGTGAGTACGAAATTGCGTGCCGCC‑TCGGCTTCGCCACCGTGCCACAGCACGGCCTCAAGCAGGTTGCGGGCGCGGcc                                                    <  3:356655/149‑1 (MQ=255)                                                                                             cccGGCTCCTTTGCGCGTTTAAAGTGAGTTCAGGAACGCGAGCAACGCAGCGCGCTGCTCGGCATCGAAAGTGAGTACGAAATTGCGTGCCGCC‑TCGGCTTCGCCACCGTGCCACAGCACGGCCTCAAGCAGGTTGCGGGCGCGGCCGt                                                  >  8:331439/1‑149 (MQ=255)                                                                                              ccGGCTCCTTTGCGCGTTTAAAGTGAGTTCAGGAACGCGAGCAACGCAGCGCGCTGCTCGGCATCGAAAGTGAGTACGAAATTGCGTGCCGCC‑TCGGCTTCGCCACCGTGCCACAGCACGGCCTCAAGCAGGTTGCGGGCGCGGCCGTc                                                 <  1:249444/149‑1 (MQ=255)                                                                                               cGGCTCCTTTGCGCGTTTAAAGTGAGTTCAGGAACGCGAGCAACGCAGCGCGCTGCTCGGCATCGAAAGTGAGTACGAAATTGCGTGCCGCC‑TCGGCTTCGCCACCGTGCCACAGCACGGCCTCAAGCAGGTTGCGGGCGCGGCCGTCa                                                <  1:40984/149‑1 (MQ=255)                                                                                                 gCTCCTTTGCGCGTTTAAAGTGAGTTCAGGAACGCGAGCAACGCAGCGCGCTGCTCGGCATCGAAAGTGAGTACGAAATTGCGTGCCGCC‑TCGGCTTCGCCACCGTGCCACAGCACGGCCTCAAGCAGGTTGCGGGCGCGGCCGTCATg                                              >  1:140744/1‑149 (MQ=255)                                                                                                  cTCCTTTGCGCGTTTAAAGTGAGTTCAGGAACGCGAGCAACGCAGCGCGCTGCTCGGCATCGAAAGTGAGTACGAAATTGCGTGCCGCC‑TCGGCTTCGCCACCGTGCCACAGCACGGCCTCAAGCAGGTTGCGGGCGGGGCCGTCATGc                                             <  6:133332/149‑1 (MQ=255)                                                                                                  cTCCTTTGCGCGTTTAAAGTGAGTTCAGGAACGCGAGCAACGCAGCGCGCTGCTCGGCATCGAAAGTGAGTACGAAATTGCGTGCCGCC‑TCGGCTTCGCCACCGTGCCACAGCACGGCCTCAAGCAGGTTGCGGGCGCGGCCGTCATg                                              >  1:253196/1‑148 (MQ=255)                                                                                                  cTCCTTTGCGCGTATAAAGTGAGTTCAGGAACGCGAGCAACGCAGCGCGCTGCTCGGCATCGGAAGTGAGTACGAAATTGCGTGCCGCC‑TCGGCTTCGCCACCGTGCCACAGCACGGGCTCAAGCAGGTTGCGGGCGCGGCGGTCATGc                                             <  8:244975/149‑1 (MQ=255)                                                                                                        tGCGCGTTTAAAGTGAGTTCAGGAACGCGAGCAACGCAGCGCGCTGCTCGGCATCGAAAGTGAGTACGAAATTGCGTGCCGCC‑TCGGCTTCGCCACCGTGCCACAGCACGGCCTCAAGCAGGTTGCGGGCGCGGCCGTCATGCAGGaa                                        <  5:17711/148‑1 (MQ=255)                                                                                                          cgcgTTTAAAGTGAGTTCAGGAACGCGAGCAACGCAGCGCGCTGCTCGGCATCGAAAGTGAGTACGAAATTGCGTGCCGCC‑TCGGCTTCGCCACCGTGCCACAGCACGGCCTCAAGCAGGTTGCGGGCGCGGCCGTCATGCAGGAACTg                                     >  1:99428/1‑149 (MQ=255)                                                                                                          cgcgTTTAAAGTGAGTTCAGGAACGCGAGCAACGCAGCGCGCTGCTCGGCATCGAAAGTGAGTACGAAATTGCGTGCCGCC‑TCGGCTTCGCCACCGTGCCACAGCACGGCCTCAAGCAGGTTGCGGGCGCGGCCGTCATGCAGGAACTg                                     <  3:312749/149‑1 (MQ=255)                                                                                                           gcgTTTAAAGTGAGTTCAGGAACGCGAGCAACGCAGCGCGCTGCTCGGCATCGAAAGTGAGTACGAAATTGCGTGCCGCC‑TCGGCTTCGCCACCGTGCCACAGCACGGCCTCAAGCAGGTTGCGGGCGCGGCCGTCATGCAGGAACTgg                                    <  2:140744/149‑1 (MQ=255)                                                                                                              tttAAAGTGAGTTCAGGAACGCGAGCAACGCAGCGCGCTGCTCGGCATCGAAAGTGAGTACGAAATTGCGTGCCGCC‑TCGGCTTCGCCACCGTGCCACAGCACGGCCTCAAGCAGGTTGCGGGCGCGGCCGTCATGCAGGAACTGGCTa                                 >  5:196319/1‑149 (MQ=255)                                                                                                                 aaaGTGAGTTCAGGAACGCGAGCAACGCAGCGCGCTGCTCGGCATCGAAAGTGAGTACGAAATTGCGTGCCGCC‑TCGGCTTCGCCACCGTGCCACAGCACGGCCTCAAGCAGGTTGCGGGCGCGGCCGTCATGCAGGAACTGGCTATgg                              >  6:98401/1‑149 (MQ=255)                                                                                                                 aaaGTGAGTTCAGGAACGCGAGCAACGCAGCGCGCTGCTCGGCATCGAAAGTGAGTACGAAATTGCGTGCCGCC‑TCGGCTTCGCCACCGTGCCACAGCACGGCCTCAAGCAGGTTGCGGGCGCGGCCGTCATGCAGGAACTGGCTATg                               <  2:174521/148‑1 (MQ=255)                                                                                                                  aaGTGAGTTCAGGAACGCGAGCAACGCAGCGCGCTGCTCGGCATCGAAAGTGAGTACGAAATTGCGTGCCGCC‑TCGGCTTCGCCACCGTGCCACAGCACGGCCTCAAGCAGGTTGCGGGCGCGGCCGTCATGCAGGAACTGGCTATGGc                             <  7:331439/149‑1 (MQ=255)                                                                                                                  aaGTGAGTTCAGGAACGCGAGCAACGCAGCGCGCTGCTCGGCATCGAAAGTGAGTACGAAATTGCGTGCCGCC‑TCGGCTTCGCCACCGTGCCACAGCACGGCCTCAAGCAGGTTGCGGGCGCGGCCGTCATGCAGGAACTGGCTATGGc                             >  7:343599/1‑149 (MQ=255)                                                                                                                    gTGAGTTCAGGAACGCGAGCAACGCAGCGCGCTGCTCGGCATCGAAAGTGAGTACGAAATTGCGTGCCGCC‑TCGGCTTCGCCACCGTGCCACAGCACGGCCTCAAGCAGGTTGCGGGCGCGGCCGTCATGCAGGAACTGGCTATGGCCa                           >  6:124814/1‑149 (MQ=255)                                                                                                                            tcgatgccgAGCAGCGCGCTGCGTTGCTCGGCATCGAAAGTGAGTACGAAATTGCGTGCCGCC‑TCGGCTTCGCCACCGTGCCACAGCACGGCCTCAAGCAGGTTGCGGGCGCGGCCGTCATGCAGGAACTGGCTATGGCCACTGACCGt                   >  5:75564/8‑149 (MQ=255)                                                                                                                             ggAACGCGAGCAACGCAGCGCGCTGCTCGGCATCGAAAGTGAGTACGAAATTGCGTGCCGCC‑TCGGCTTCGCCACCGTGCCACAGCACGGCCTCAAGCAGGTTGCGGGCGCGGCCGTCATGCAGGAACTGGCTATGGCCACTGACCGtt                  <  6:196319/149‑1 (MQ=255)                                                                                                                              gAACGCGAGCAACGCAGCGCGCTGCTCGGCATCGAAAGTGAGTACGAAATTGCGTGCCGCC‑TCGGCTTCGCCACCGTGCCACAGCACGGCCTCAAGCAGGTTGCGGGCGCGGCCGTCATGCAGGAACTGGCTATGGCCACTGACCGttt                 <  8:343599/149‑1 (MQ=255)                                                                                                                                aCGCGAGCAACGCAGCGCGCTGCTCGGCATCGAAAGTGAGTACGAAATTGCGTGCCGCC‑TCGGCTTCGCCACCGTGCCACAGCACGGCCTCAAGCAGGTTGCGGGCGCGGCCGTCATGCAGGAACTGGCTATGGCCACTGACCGTTTCg               <  3:362499/149‑1 (MQ=255)                                                                                                                                aCGCGAGCAACGCAGCGCGCTGCTCGGCATCGAAAGTGAGTACGAAATTGCGTGCCGCC‑TCGGCTTCGCCACCGTGCCACAGCACGGCCTCAAGCAGGTTGCGGGCGCGGCCGTCATGCAGGAACTGGCTATGGCCACTGACCGTTTCg               >  2:46981/1‑149 (MQ=255)                                                                                                                                aCGCGAGCAACGCAGCGCGCTGCTCGGCATCGAAAGTGAGTACGAAATTGCGTGCCGCC‑TCGGCTTCGCCACCGTGCCACAGCACGGCCTCAAGAAGGTTGCGGGCGCGGCCGTCATGCAGGAACTGGCTATGGCCACTGACCGTTTCg               >  1:53392/1‑149 (MQ=255)                                                                                                                                 cgcgTGCAACGCAGCGCGCTGCTCGGCATCGAAAGTGAGTACGAAATTGCGTGCCGCC‑TCGGCTTCGCCACCGTGCCACAGCACGGCCTCAAGCAGGTTGCGGGCGCGGCCGTCATGCAGGAACTGGCTATGGCCACTGACCGTTTCGc              <  8:6455/149‑1 (MQ=255)                                                                                                                                 cgcgAGCAACGCAGCGCGCTGCTCGGCATCGAAAGTGAGTACGAAATTGCGTGCCGCCAGCGGCTTCGCCACCGTGCCACAGCACGGCCTCAAGCAGGTTGCGGGCGCGGCCGTCATGCAGGAACTGGCTATGGCCACTGACCGTTTCg               >  1:297460/1‑149 (MQ=255)                                                                                                                                   cgAGCAGCGCGCTGCGTTGCTCGGCATCGAAAGTGAGTACGAAATTGCGTGCCGCC‑TCGGCTTCGCCACCGTGCCACAGCACGGCCTCAAGCAGGTTGCGGGCGCGGCCGTCATGCAGGAACTGGCTATGGCCACTGACCGTTTCGCt             >  2:275049/1‑148 (MQ=255)                                                                                                                                   cgAGCAGCGCGCTGCGTTGCTCGGCATCGAAAGTGAGTACGAAATTGCGTGCCGCC‑TCGGCTTCGCCACCGTGCCACAGCACGGCCTCAAGCAGGTTGCGGGCGCGGCCGTCATGCAGGAACTGGCTATGGCCACTGACCGTTTCGCt             >  3:334903/1‑148 (MQ=255)                                                                                                                                    gAGCAACGCAGCGCGCTGCTCGGCATCGAAAGTGAGTACGAAATTGCGTGCCGCC‑TCGGCTTCGCCACCGTGCCACAGCACGGCCTCAAGCAGGTTGCGGGCGCGGCCGTCATGCAGGAACTGGCTATGGCCACTGACCGTTTCGCt             >  3:119202/1‑147 (MQ=255)                                                                                                                                      gCAACGCAGCGCGCTGCTCGGCATCGAAAGTGAGTACGAAATTGCGTGCCGCC‑TCGGCTTCGCCACCGTGCCACAGCACGGCCTCAAGCAGGTTGCGGGCGCGGCCGTCATGCAGGAACTGGCTATGGCCACTGACCGTTTCGCTCAgg         <  8:124433/149‑1 (MQ=255)                                                                                                                                             aGCGCGCTGCTCGGCATCGAAAGTGAGTACGAAATTGCGTGCCGCC‑TCGGCTTCGCCACCGTGCCACAGCACGGCCTCAAGCAGGTTGCGGGCGCGGCCGTCATGCAGGAACTGGCTATGGCCACTGACCGTTTCGCTCAGGCCGATGc  <  5:308799/149‑1 (MQ=255)                                                                                                                                               |                                                                                                                                                    CGGCGGAGGTCACTGCTTGCGGGTCTTGTGGCGAGCAGGCACCGAGGGCGAGTGCGGCGAGGCTGGTGAACAACAGTTTGGGTCGGAACATGCCCGGCTCCTTTGCGCGTTTAAAGTGAGTTCAGGAACGCGAGCAACGCAGCGCGCTGCTCGGCATCGAAAGTGAGTACGAAATTGCGTGCCGCC‑TCGGCTTCGCCACCGTGCCACAGCACGGCCTCAAGCAGGTTGCGGGCGCGGCCGTCATGCAGGAACTGGCTATGGCCACTGACCGTTTCGCTCAGGCCGATGC  >  NC_002947/1051829‑1052117

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑