Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,054,463 C→T 6.7% M1M (GTG→ATG) †‡ PP_0912 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,054,4630CT 6.7% 263.6 / 5.4 89M1M (GTG→ATG) †‡PP_0912hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (40/43);  new base T (2/4);  total (42/47)
Fisher's exact test for biased strand distribution p-value = 6.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01

GACGATTCGGCCTTCGCCCCAGACATCAAGAATCTGCAGCACTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGACCCGGTCTGCTTGACCAGGGCGGCGATGGCGTCCCG  >  NC_002947/1054317‑1054607
                                                                                                                                                  |                                                                                                                                                
gACGATTCGGCCTTCGCCCCAGACATCAAGAATCTGCAGCACTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACaa                                                                                                                                                <  5:151214/149‑1 (MQ=255)
     ttCGGCCTTCGCCCCAGACATCAAGAATCTGCAGCACTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCg                                                                                                                                           >  3:353328/1‑149 (MQ=255)
        ggCCTTCGCCCCAGACATCAAGAATCTGCAGCACTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGc                                                                                                                                        >  8:170113/1‑149 (MQ=255)
         tccTTCGCACCAGACATCAAGAATCTGCAGCACTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCACCAAACGAAAACGCCCGGCACAAGGCCGGGCg                                                                                                                                       >  2:39261/2‑149 (MQ=255)
              cGCCCCAGACATCAAGAATCTGCAGCACTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGGAAACGAAAACGCCCGGCCTTGTGCCGGGCGttt                                                                                                                                    <  1:241826/147‑1 (MQ=255)
              cGCCCCAGACATCAAGAATCTGCAGCACTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTg                                                                                                                                   <  7:299737/148‑1 (MQ=255)
               gCCCCAGACATCAAGAATCTGCAGCACTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCCTTGTGCCGGGCGttttc                                                                                                                                  <  4:172800/148‑3 (MQ=255)
               gCCCCAGACATCAAGAATCTGCAGCACTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCg                                                                                                                                 <  5:110636/149‑1 (MQ=255)
                ccccAGACATCAAGAATCTGCAGCACTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCCTTGTGCCGGGCGTTTTCgt                                                                                                                                <  5:332071/149‑2 (MQ=255)
                ccccAGACATCAAGAATCTGCAGCACTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCg                                                                                                                                 <  7:122757/148‑1 (MQ=255)
                ccccAGACATCAAGAATCTGCAGCACTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCg                                                                                                                                 >  8:122757/1‑148 (MQ=255)
                 cccAGACATCAAGAATCTGCAGCACTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGt                                                                                                                               >  4:359487/1‑149 (MQ=255)
                 cccAGACATCAAGAATCTGCAGCACTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGt                                                                                                                               >  6:4687/1‑149 (MQ=255)
                 cccAGACATCAAGAATCTGCAGCACTATGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAg                                                                                                                                               <  1:327445/133‑1 (MQ=255)
                 cccAGACATCAAGAATCTGCAGCACTATGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAg                                                                                                                                               >  2:327445/1‑133 (MQ=255)
                   cAGACATCAAGAATCTGCAGCACTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTg                                                                                                                              >  2:21627/1‑148 (MQ=255)
                    aGACATCAAGAATCTGCAGCACTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGaa                                                                                                                            <  1:160019/149‑1 (MQ=255)
                        aTCAAGAATCTGCAGCACTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAgcgc                                                                                                                        >  6:22381/1‑149 (MQ=255)
                          cAAGAATCTGCAGCACTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGTAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTa                                                                                                                      >  6:23010/1‑149 (MQ=255)
                           aaGAATCTGCAGCACTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGACCGGGCGTTTGCGGTGAAGCGCTa                                                                                                                      <  3:273218/148‑1 (MQ=255)
                             gAATCTGCAGCACTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTAcag                                                                                                                   <  4:211963/149‑1 (MQ=255)
                             gAATCTGCAGCACTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGAATCTGCCTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTAcag                                                                                                                   >  1:289462/1‑149 (MQ=255)
                               aTCTGCAGCACTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTAcagca                                                                                                                 <  4:130609/149‑1 (MQ=255)
                                tCTGCAGCACTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTAcagcag                                                                                                                >  5:233235/1‑149 (MQ=255)
                                    cagcaCTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGCAAACGCCCGGCCTTGTGCCGGGCGTTTTCgtt                                                                                                                               >  5:68680/1‑128 (MQ=38)
                                    cagcaCTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGCAAACGCCCGGCCTTGTGCCGGGCGTTTTCgtt                                                                                                                               <  6:68680/130‑3 (MQ=38)
                                    cagcaCTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAgg                                                                                                               <  5:200882/146‑1 (MQ=255)
                                    cagcaCTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGAt                                                                                                             <  6:233235/148‑1 (MQ=255)
                                        aCTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGaa                                                                                                        <  4:122454/149‑1 (MQ=255)
                                           gtgCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTc                                                                                                     >  7:219308/1‑149 (MQ=255)
                                            tgCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTc                                                                                                     <  7:170113/148‑1 (MQ=255)
                                            tgCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCg                                                                                                    <  8:283917/149‑1 (MQ=255)
                                                tcctGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGAt                                                                                                >  4:4918/1‑149 (MQ=255)
                                                 cctGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATc                                                                                               >  4:207256/1‑149 (MQ=255)
                                                    gTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTAca                                                                                                                    <  7:308347/125‑1 (MQ=255)
                                                    gTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTAca                                                                                                                    >  8:308347/1‑125 (MQ=255)
                                                     ttAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCg                                                                                             >  8:120471/1‑147 (MQ=255)
                                                     ttAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCg                                                                                             >  4:750/1‑147 (MQ=255)
                                                     ttAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCa                                                                                           <  8:70073/149‑1 (MQ=255)
                                                          tttGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTAca                                                                                                                    >  8:300129/1‑119 (MQ=255)
                                                          tttGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTAca                                                                                                                    <  7:300129/119‑1 (MQ=255)
                                                          tttGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTc                                                                                      >  4:145657/1‑149 (MQ=255)
                                                              aTCGAGGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCggg                                                                                  >  8:260680/1‑149 (MQ=255)
                                                                cGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCgg                                                                                   <  2:261048/146‑1 (MQ=255)
                                                                cGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCgg                                                                                   >  1:261048/1‑146 (MQ=255)
                                                                  aaGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCa                                                                              <  5:22381/149‑1 (MQ=255)
                                                                   aGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAg                                                                             <  7:120471/149‑1 (MQ=255)
                                                                      ccTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGtt                                                                          >  8:311812/1‑149 (MQ=255)
                                                                       cTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTg                                                                         <  3:145657/149‑1 (MQ=255)
                                                                        tACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCgg                                                                                   >  1:19043/1‑138 (MQ=255)
                                                                        tACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCgg                                                                                   <  2:19043/138‑1 (MQ=255)
                                                                          cAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGc                                                                       <  2:289462/148‑1 (MQ=255)
                                                                           aGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCaa                                                                     >  5:25899/1‑149 (MQ=255)
                                                                             gATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCCTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATg                                                                   >  4:286287/1‑149 (MQ=255)
                                                                                 aGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCa                                                               <  7:108827/149‑1 (MQ=255)
                                                                                 aGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCa                                                               <  7:311812/149‑1 (MQ=255)
                                                                                           tGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGc                                                     <  1:173826/149‑1 (MQ=255)
                                                                                           tGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGc                                                     <  5:131691/149‑1 (MQ=255)
                                                                                             cACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCa                                                    <  5:4687/148‑1 (MQ=255)
                                                                                             cACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAg                                                   <  3:207256/149‑1 (MQ=255)
                                                                                                 ccAGGCAGCGGGAAGCGTTTTACCCGGCATCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTg                                               >  1:24730/1‑149 (MQ=255)
                                                                                                     gCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGt                                                                                                   <  5:79698/93‑1 (MQ=255)
                                                                                                     gCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGt                                                                                                   >  6:79698/1‑93 (MQ=255)
                                                                                                       aGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTtcgat                                         >  1:75705/1‑149 (MQ=255)
                                                                                                       aGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTtcgat                                         <  1:39261/149‑1 (MQ=255)
                                                                                                           ggAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGAc                                     <  1:21627/149‑1 (MQ=255)
                                                                                                              aGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGACCCg                                  >  3:331819/1‑149 (MQ=255)
                                                                                                                       cccGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTtcgatc                                        >  6:96970/1‑134 (MQ=255)
                                                                                                                       cccGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTtcgatc                                        <  5:96970/134‑1 (MQ=255)
                                                                                                                        ccGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGACCCGGTCTGCTTGa                        >  3:138877/1‑149 (MQ=255)
                                                                                                                         cGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGACCCGGTCTGCTTGAc                       >  8:227239/1‑149 (MQ=255)
                                                                                                                           gcagcaAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGACCCGGTCTGCTTGACCa                     <  3:4918/149‑1 (MQ=255)
                                                                                                                             agcaAACGAACACGCCCGGAACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGACCCGGTCTGCTTGACCAgg                   <  4:205083/149‑1 (MQ=255)
                                                                                                                             agcaAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGACCCGGTCTGCTTGACCAgg                   >  3:195626/1‑149 (MQ=255)
                                                                                                                              gcaAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGACCCGGTCTGCTTGACCAggg                  <  8:219308/149‑1 (MQ=255)
                                                                                                                               caAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTtc                                            >  7:314921/1‑122 (MQ=255)
                                                                                                                               caAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTtc                                            <  8:314921/122‑1 (MQ=255)
                                                                                                                               caAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGACCCGGTCTGCTTGACCAGggc                 >  1:38258/1‑149 (MQ=255)
                                                                                                                                aaaCGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGACCCGGTCTGCTTGACCAGggcg                <  3:359487/149‑1 (MQ=255)
                                                                                                                                 aaCGAAACCGCCCGGCACAAGGCCGGGGGTTTGCGGTGAAGCGCTACAGCAGCCTCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGACCCGGTCTGCTTGACCAGggcgg               <  3:750/149‑1 (MQ=255)
                                                                                                                                   cgcaaaCGCCCGGCCTTGTGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGACCCGGTCTGCTTGACCAGggcggc              >  7:86690/4‑148 (MQ=255)
                                                                                                                                    gAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGGTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGACCCGGTCTGCTTGACCAGGGCGGCGa            <  7:260680/149‑1 (MQ=255)
                                                                                                                                      aaaCGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGACCCGGTCTGCTTGACCAGGGCGGCGATg          >  4:206985/1‑149 (MQ=255)
                                                                                                                                        aCGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGACCCGGTCTGCTTGACCAGGGCGGCGATGGc        >  3:100753/1‑149 (MQ=255)
                                                                                                                                        aCGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGACCCGGTCTGCTTGACCAGGGCGGCGATGGc        >  1:209557/1‑149 (MQ=255)
                                                                                                                                           cccGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGACCCGGTCTGCTTGACCAGGGCGGCGATGGCGTc     <  4:331819/149‑1 (MQ=255)
                                                                                                                                            ccGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGACCCGGTCTGCTTGACCAGGGCGGCGATGGCGTcc    >  2:259972/1‑149 (MQ=255)
                                                                                                                                            ccGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGACCCGGTCTGCTTGACCAGGGCGGCGATGGCGTcc    <  3:286287/149‑1 (MQ=255)
                                                                                                                                              ggCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGACCCGGTCTGCTTGACCAGGGCGGCGATGGCGTCCCg  <  4:59451/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
GACGATTCGGCCTTCGCCCCAGACATCAAGAATCTGCAGCACTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGACCCGGTCTGCTTGACCAGGGCGGCGATGGCGTCCCG  >  NC_002947/1054317‑1054607

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑