Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,058,712 C→A 5.7% S59I (AGC→ATC)  sodB ← superoxide dismutase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,058,7120CA 5.7% 283.7 / 3.8 87S59I (AGC→ATC) sodBsuperoxide dismutase
Reads supporting (aligned to +/- strand):  ref base C (38/44);  new base A (3/2);  total (41/46)
Fisher's exact test for biased strand distribution p-value = 6.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

TTGAACTTGTCGAAGGAACCGAAAGCGGCGTTGATGGCGTCAGCCAGTGCACCGGTAGGCTGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGTGTTGTGGTGCTTGTCGTGGTGATACTCCAGGGTTTCCTTGGAGATGTGCGGCTGCAGGGCATCATGG  >  NC_002947/1058565‑1058855
                                                                                                                                                   |                                                                                                                                               
ttGAACTTGTCGAAGGAACCGAAAGCGGCGTTGATGGCGTCAGCCAGTGCACCGGTAGGCTGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGct                                                                                                                                                >  6:206837/1‑149 (MQ=255)
ttGAACTTGTCGAAGGAACCGAAAGCGGCGTTGATGGCGTCAGCCAGTGCACCGGTAGGCTGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGc                                                                                                                                                 >  5:227986/1‑148 (MQ=255)
ttGAACTTGTCGAAGGAACCGAAAGCGGCGTTGATGGCGTCAGCCAGTGCACCGGTAGGCTGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGc                                                                                                                                                 >  2:367950/1‑148 (MQ=255)
ttGAACTTGTCGAAGGAACCGAAAGCGGCGTTGATGGCGTCAGCCAGTGCACCGGTAGGCTGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGc                                                                                                                                                 >  7:327400/1‑148 (MQ=255)
  gAACTTGTCGAAGGAACCGAAAGCGGCGTTGATGGCGTCAGCCAGTGCACCGGTAGGCTGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGctct                                                                                                                                              >  5:7306/1‑149 (MQ=255)
   aaCTTGTCGAAGGAACCGAAAGCGGCGTTGATGGCGTCAGCCAGTGCACCGGTAGGCTGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCtt                                                                                                                                             >  4:179189/1‑149 (MQ=255)
     cTTGTCGAAGGAACCGAAAGCGGCGTTGATGGCGTCAGCCAGTGCACCGGTAGGCTGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGAGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGa                                                                                                                                           >  6:59509/1‑149 (MQ=255)
        gTCGAAGGAACCGAAAGCGGCGTTGATGGCGTCAGCCAGTGCACCGGTAGGCTGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTACAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGa                                                                                                                                        >  2:264348/1‑149 (MQ=255)
         tCGAAGGAACCGAAAGCGGCGTTGATGGTGTCAGCCAGTGCACCGGTAGGCTGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCACGAAGAGCTCTTGACGa                                                                                                                                        >  7:28717/1‑148 (MQ=255)
         tCGAAGGAACCGAAAGCGGCGTTGATGGCGTCAGCCAGTGCACCGGTAGGCTGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGAt                                                                                                                                       >  7:67226/1‑149 (MQ=255)
          cGAAGGAACCGAAAGCGGCGTTGATGGCGTCAGCCAGTGCACCGGTAGGCTGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGAtc                                                                                                                                      >  4:200028/1‑149 (MQ=255)
           gAAGGAACCGAAAGCGGCGTTGATGGCGTCAGCCAGTGCACCGGTAGGCTGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGAtct                                                                                                                                     >  4:206792/1‑149 (MQ=255)
            aaGGAACCGAAACCGGCGTTGATGGCGTCAGCCAGTGCACCGGTACGCAGGCCGCCGACGATTCGCGACAGGCAGTTCCAGTAGAAGGTCTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTATTGACGAtctc                                                                                                                                    >  4:173054/1‑149 (MQ=255)
             aGGAACCGAAAGCGGCGTTGATGGCGTCAGCCAGTGCACCGGTAGGCTGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATctct                                                                                                                                   <  4:346597/149‑1 (MQ=255)
                  ccGAAAGCGGCGTTGATGGCGTCAGCCAGTGCACCGGTAGGCTGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGATCGTCAAGAGCTCTTcggg                                                                                                                              <  8:13169/149‑4 (MQ=255)
                   cGAAAGCGGCGTTGATGGCGTCAGCCAGTGCACCGGTAGGCTGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAgg                                                                                                                             <  7:133211/149‑1 (MQ=255)
                   cGAAAGCGGCGTTGATGGCGTCAGCCAGTGCACCGGTAGGCTGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAgg                                                                                                                             <  3:108647/149‑1 (MQ=255)
                   cGAAAGCGGCGTTGATGGCGTCAGCCAGTGCACAGGAAGGCTGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGATCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGACCTCTTGACGATCTCTTCAAgg                                                                                                                             >  5:205494/1‑149 (MQ=255)
                    gAAAGCGGCGTTGATGGCGTCAGCCAGTGCACCGGTAGGCTGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAggg                                                                                                                            >  3:346731/1‑149 (MQ=255)
                         cggcgTTGATGGCGTCAGCCAGTGCACCGGTAGGCTGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTg                                                                                                                       <  1:367950/149‑1 (MQ=255)
                          ggcgTTGATGGCGTCAGCCAGTGCACCGGTAGGCTGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGAtc                                                                                                                                      <  7:248422/133‑1 (MQ=255)
                          ggcgTTGATGGCGTCAGCCAGTGCACCGGTAGGCTGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGAtc                                                                                                                                      >  8:248422/1‑133 (MQ=255)
                            cgTTGATGGCGTCAGCCAGTGCACCGGTAGGCTGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCt                                                                                                                    <  3:206792/149‑1 (MQ=255)
                             gTTGATGGTGTCAGCCAGTGCACCGGTAGGCTGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCtt                                                                                                                   <  8:28717/149‑1 (MQ=255)
                                  tGGCGTCAGCCAGTGCACCGGTAGGCTGGCCGCCGCCGTTTGGCGACAAGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAAt                                                                                                              >  1:371942/1‑149 (MQ=255)
                                     cGTGAGCCAGTGCACCGGTAGGCTGGCCGCCGCCGTTTGGCGACTGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCg                                                                                                           <  1:173638/149‑1 (MQ=255)
                                     cGTCAGCCAGTGCACCGGTAGGCTGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCg                                                                                                           <  3:179189/149‑1 (MQ=255)
                                        cAGCCAGTGCACCGGTAGGCTGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGGAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTg                                                                                                        >  1:317509/1‑149 (MQ=255)
                                         aGCCAGTGCACCGGTAGGCTGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTg                                                                                                        <  5:239410/148‑1 (MQ=255)
                                          gCCAGTGCACCGGTAGGCTGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGcc                                                                                                      >  3:289792/1‑149 (MQ=255)
                                          gCCAGTGCACCGGTAGGCTGGACGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGcc                                                                                                      >  3:272599/1‑149 (MQ=255)
                                           ccAGTGCACCGGTAGGCTGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTc                                                                                                            <  8:319058/142‑1 (MQ=255)
                                           ccAGTGCACCGGTAGGCTGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTc                                                                                                            >  7:319058/1‑142 (MQ=255)
                                                  aCCGGTAGGCTGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGAc                                                                                                >  8:192102/1‑147 (MQ=255)
                                                  aCCGGTAGGCTGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGAc                                                                                                >  5:269123/1‑147 (MQ=255)
                                                    cggtgcacTGGCTGACGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAgg                                                                                            <  1:171379/142‑1 (MQ=255)
                                                    cGGTAGGCTGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGGAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAgg                                                                                            <  2:317509/149‑1 (MQ=255)
                                                    cGGTAGGCTGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTgg                                                                                                   <  5:286637/142‑1 (MQ=255)
                                                    cGGTAGGCTGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTgg                                                                                                   >  6:286637/1‑142 (MQ=255)
                                                    cGGTAGGCTGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAgg                                                                                            <  6:141817/149‑1 (MQ=255)
                                                    cGGTAGGCTGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAgg                                                                                            <  7:192102/149‑1 (MQ=255)
                                                          gCTGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTc                                                                                      <  1:264348/149‑1 (MQ=255)
                                                            tGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAg                                                                                    >  3:28408/1‑149 (MQ=255)
                                                            tGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAg                                                                                    >  1:141890/1‑149 (MQ=255)
                                                              gccgccgccGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGt                                                                                  >  2:182474/1‑149 (MQ=255)
                                                               ccgccgccgTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGtt                                                                                 <  8:327400/149‑1 (MQ=255)
                                                                cgccgccgTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAg                                                                                    <  4:208537/145‑1 (MQ=255)
                                                                cgccgccgTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAg                                                                                    >  3:208537/1‑145 (MQ=255)
                                                                 gccgccgTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTAGGTGCCTGGGACCAGGTTGTTCAGGTTCa                                                                               >  1:205447/1‑149 (MQ=255)
                                                                  ccgccgTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCAc                                                                              <  4:329888/149‑1 (MQ=255)
                                                                  ccgccgTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCAc                                                                              >  2:358089/1‑149 (MQ=255)
                                                                    gccgTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGa                                                                            <  3:89350/149‑1 (MQ=255)
                                                                                cAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGtgttgt                                                                <  4:28408/149‑1 (MQ=255)
                                                                                 aGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGTGTtgtg                                                               >  1:24830/1‑149 (MQ=255)
                                                                                  ggCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGTGTTgtgg                                                              <  5:206837/149‑1 (MQ=255)
                                                                                   gctgTTCAAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCACGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCATGTTCACGACATAGGTGTTgtggt                                                             <  6:205494/146‑1 (MQ=255)
                                                                                         ccAGTAGAAGGTGTGGTTCCAGTCTTGAGCGGCGTTGTTGAAGATGCGGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTGGAATTCGGTGCCTGGGACCATGTTGTTCAGGTTCACGACATAGGTGTTGTGGTGCTTg                                                        <  5:95162/148‑1 (MQ=255)
                                                                                             tAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGTGTTGTGGTGCTTGTCgtg                                                   >  3:362326/1‑149 (MQ=255)
                                                                                               gAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGTGTTGTGGTGCTTGTCgtggt                                                 <  4:289792/149‑1 (MQ=255)
                                                                                               gAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGTGTTGTGGTGCTTGTCgtgg                                                  <  8:67226/148‑1 (MQ=255)
                                                                                               gAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGTGTTGTGGTGCTTGTCgtgg                                                  <  4:216008/148‑1 (MQ=255)
                                                                                               gAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGTGTTGTGGTGCTTGTCgtgg                                                  <  6:7306/148‑1 (MQ=255)
                                                                                               gAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGTGTTGTGGTGCTTGTCgtgg                                                  >  3:216008/1‑148 (MQ=255)
                                                                                                 aGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGTGTTGTGGTGCTTGTCGTGGTGa                                               <  5:59509/149‑1 (MQ=255)
                                                                                                 aGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGTGTTGTGGTGCTTGTCGTGGTGa                                               <  2:24830/149‑1 (MQ=255)
                                                                                                       ggTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGTGTTGTGGTGCTTGTCGTGGTGATACTc                                          >  2:145169/1‑148 (MQ=255)
                                                                                                         ttCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGTGTTGTGGTGCTTGTCGTGGTGa                                               <  2:265985/141‑1 (MQ=255)
                                                                                                         ttCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCATGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGTGTTGTGGTGCTTGTCGTGGTGa                                               >  1:265985/1‑141 (MQ=255)
                                                                                                           ccAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGTGTTGTGGTGCTTGTCGTGGTGATACTCCAggg                                     >  3:175401/1‑149 (MQ=255)
                                                                                                           ccAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGTGTTGTGGTGCTTGTCGTGGTGATACTCCAgg                                      <  4:346731/148‑1 (MQ=255)
                                                                                                           ccAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGTGTTGTGGTGCTTGTCGTGGTGATAATCAAggg                                     >  4:25937/1‑149 (MQ=255)
                                                                                                            cAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGTGTTGTGGTGCTTGTCGTGGTGATACTCCAGGGt                                    >  2:304639/1‑149 (MQ=255)
                                                                                                             aGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGTGTTGTGGTGCTTGTCGTGGTGATACTCCAGGGtt                                   <  6:269123/149‑1 (MQ=255)
                                                                                                                 ttGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGTGTTGTGGTGCTTGTCGTGGTGATACTCCAGGGTTTCCt                               >  5:241312/1‑149 (MQ=255)
                                                                                                                   gAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGTGTTGTGGTGCTTGTCgtggt                                                 >  4:133333/1‑129 (MQ=255)
                                                                                                                   gAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGTGTTGTGGTGCTTGTCgtggt                                                 <  3:133333/129‑1 (MQ=255)
                                                                                                                     gcggcgTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGTGTTGTGGTGCTTGTCGTGGTGATACTCCAGGGTTTCCTTGga                           <  4:362326/149‑1 (MQ=255)
                                                                                                                                  aGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGTGTTGTGGTGCTTGTCGTGGTGATACTCCAGGGTTTCCTTGGAGATGTGCGGCTGc              <  1:358089/149‑1 (MQ=255)
                                                                                                                                   gATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGTGTTGTGGTGCTTGTCGTGGTGATACTCCAGGGTTTCCTTGGAGATGTGCGGCTGCa             <  2:321196/149‑1 (MQ=255)
                                                                                                                                    aTGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGTGTTGTGGTGCTTGTCGTGGTGATACTCCAGGGTTTCCTTGGAGATGTGCGGCTGCAg            <  3:200028/149‑1 (MQ=255)
                                                                                                                                      gccgccCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGTGTTGTGGTGCTTGTCGTGGTGATACTCCAGGGTTTCCTTGGAGATGTGCGGCTGCAgg           <  6:227986/148‑1 (MQ=255)
                                                                                                                                      gccgccCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCCAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGTGTTGTGGTGCTTGTCGTGGTGATACTCCAGGGTTTCCTTGGAGATGTGCGGCTGCAggg          >  1:265303/1‑149 (MQ=255)
                                                                                                                                       ccgccCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGTGTTGTGGTGCTTGTCGTGGTGATACTCCAGGGTTTCCTTGGAGATGTGCGGCTGCAGGGc         <  3:173054/149‑1 (MQ=255)
                                                                                                                                         gccCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGTGTTGTGGTGCTTGTCGTGGTGATACTCCAGGGTTTCCTTGGAGATGTGCGGCTGCAGGGcat       >  1:36797/1‑149 (MQ=255)
                                                                                                                                          ctggAAGAGATCGTCAAGAGCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGTGTTGTGGTGCTTGTCgtggt                                                 <  6:314191/103‑1 (MQ=255)
                                                                                                                                          ctggAAGAGATCGTCAAGAGCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGTGTTGTGGTGCTTGTCgtggt                                                 >  5:314191/4‑106 (MQ=255)
                                                                                                                                          ctggAAGAGATCGTCAAGAGCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGTGTTGTGGTGCTTGTCGTGGTGATACTCCAGGGTTTCCTTGGAGATGTGCGGCTGCAGGGcat       >  3:79897/4‑148 (MQ=255)
                                                                                                                                          cccGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGTGTTGTGGTGCTTGTCGTGGTGATACTCCAGTGTTTCCTTGGAGATGTGCGGCTGCAGGGcatc      <  3:25937/149‑1 (MQ=255)
                                                                                                                                          cccGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGTGTTGTGGTGCTTGTCGTGGTGATACTCCAGGGTTTCCTTGGAGATGTGCGGCTGCAGGGcat       >  4:6497/1‑148 (MQ=255)
                                                                                                                                           tggAAGAGATCGTCAAGAGCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGTGTTGTGGTGCTTGTCGTGGTGATACTCCAGGGTTTCCTTGGAGATGTGCGGCTGCAGGGcatca     >  4:82374/3‑149 (MQ=255)
                                                                                                                                              ccccgCTCTTGCCGATCTCTTCCAGGGGCTTGCCTGCGAATGCGGTGCCTGGGCCCAGGGTGTTCAGGGTCACGACATAGGTGTTGTGGTGCTTGTCGTGGTGATACTCCAGGGTTTCCTTGGTGATGTGCGGCTGCAGGGCATCATgg  <  7:339313/145‑1 (MQ=255)
                                                                                                                                              aaGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGTGTTGTGGTGCTTGTCGTGGTGATACTCCAGGGTTTCCTTGGAGATGTGCGGCTGCAGGGCATCATgg  <  1:332911/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
TTGAACTTGTCGAAGGAACCGAAAGCGGCGTTGATGGCGTCAGCCAGTGCACCGGTAGGCTGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGTGTTGTGGTGCTTGTCGTGGTGATACTCCAGGGTTTCCTTGGAGATGTGCGGCTGCAGGGCATCATGG  >  NC_002947/1058565‑1058855

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑