Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,058,715 T→G 5.9% K58T (AAG→ACG)  sodB ← superoxide dismutase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,058,7150TG 5.9% 268.7 / 6.7 86K58T (AAG→ACG) sodBsuperoxide dismutase
Reads supporting (aligned to +/- strand):  ref base T (36/44);  new base G (3/2);  total (40/46)
Fisher's exact test for biased strand distribution p-value = 6.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.59e-01

GAACTTGTCGAAGGAACCGAAAGCGGCGTTGATGGCGTCAGCCAGTGCACCGGTAGGCTGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGTGTTGTGGTGCTTGTCGTGGTGATACTCCAGGGTTTCCTTGGAGATGTGCGGCTGCAGGGCATCATGG  >  NC_002947/1058567‑1058855
                                                                                                                                                    |                                                                                                                                            
gAACTTGTCGAAGGAACCGAAAGCGGCGTTGATGGCGTCAGCCAGTGCACCGGTAGGCTGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGctct                                                                                                                                              >  5:7306/1‑149 (MQ=255)
 aaCTTGTCGAAGGAACCGAAAGCGGCGTTGATGGCGTCAGCCAGTGCACCGGTAGGCTGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCtt                                                                                                                                             >  4:179189/1‑149 (MQ=255)
   cTTGTCGAAGGAACCGAAAGCGGCGTTGATGGCGTCAGCCAGTGCACCGGTAGGCTGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGAGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGa                                                                                                                                           >  6:59509/1‑149 (MQ=255)
      gTCGAAGGAACCGAAAGCGGCGTTGATGGCGTCAGCCAGTGCACCGGTAGGCTGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTACAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGa                                                                                                                                        >  2:264348/1‑149 (MQ=255)
       tCGAAGGAACCGAAAGCGGCGTTGATGGTGTCAGCCAGTGCACCGGTAGGCTGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCACGAAGAGCTCTTGACGa                                                                                                                                        >  7:28717/1‑148 (MQ=255)
       tCGAAGGAACCGAAAGCGGCGTTGATGGCGTCAGCCAGTGCACCGGTAGGCTGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGAt                                                                                                                                       >  7:67226/1‑149 (MQ=255)
        cGAAGGAACCGAAAGCGGCGTTGATGGCGTCAGCCAGTGCACCGGTAGGCTGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGAtc                                                                                                                                      >  4:200028/1‑149 (MQ=255)
         gAAGGAACCGAAAGCGGCGTTGATGGCGTCAGCCAGTGCACCGGTAGGCTGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGAtct                                                                                                                                     >  4:206792/1‑149 (MQ=255)
          aaGGAACCGAAACCGGCGTTGATGGCGTCAGCCAGTGCACCGGTACGCAGGCCGCCGACGATTCGCGACAGGCAGTTCCAGTAGAAGGTCTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTATTGACGAtctc                                                                                                                                    >  4:173054/1‑149 (MQ=255)
           aGGAACCGAAAGCGGCGTTGATGGCGTCAGCCAGTGCACCGGTAGGCTGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATctct                                                                                                                                   <  4:346597/149‑1 (MQ=255)
                ccGAAAGCGGCGTTGATGGCGTCAGCCAGTGCACCGGTAGGCTGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGATCGTCAAGAGCTCTTcggg                                                                                                                              <  8:13169/149‑4 (MQ=255)
                 cGAAAGCGGCGTTGATGGCGTCAGCCAGTGCACCGGTAGGCTGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAgg                                                                                                                             <  3:108647/149‑1 (MQ=255)
                 cGAAAGCGGCGTTGATGGCGTCAGCCAGTGCACCGGTAGGCTGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAgg                                                                                                                             <  7:133211/149‑1 (MQ=255)
                 cGAAAGCGGCGTTGATGGCGTCAGCCAGTGCACAGGAAGGCTGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGATCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGACCTCTTGACGATCTCTTCAAgg                                                                                                                             >  5:205494/1‑149 (MQ=255)
                  gAAAGCGGCGTTGATGGCGTCAGCCAGTGCACCGGTAGGCTGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAggg                                                                                                                            >  3:346731/1‑149 (MQ=255)
                       cggcgTTGATGGCGTCAGCCAGTGCACCGGTAGGCTGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTg                                                                                                                       <  1:367950/149‑1 (MQ=255)
                        ggcgTTGATGGCGTCAGCCAGTGCACCGGTAGGCTGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGAtc                                                                                                                                      <  7:248422/133‑1 (MQ=255)
                        ggcgTTGATGGCGTCAGCCAGTGCACCGGTAGGCTGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGAtc                                                                                                                                      >  8:248422/1‑133 (MQ=255)
                          cgTTGATGGCGTCAGCCAGTGCACCGGTAGGCTGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCt                                                                                                                    <  3:206792/149‑1 (MQ=255)
                           gTTGATGGTGTCAGCCAGTGCACCGGTAGGCTGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCtt                                                                                                                   <  8:28717/149‑1 (MQ=255)
                                tGGCGTCAGCCAGTGCACCGGTAGGCTGGCCGCCGCCGTTTGGCGACAAGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAAt                                                                                                              >  1:371942/1‑149 (MQ=255)
                                   cGTGAGCCAGTGCACCGGTAGGCTGGCCGCCGCCGTTTGGCGACTGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCg                                                                                                           <  1:173638/149‑1 (MQ=255)
                                   cGTCAGCCAGTGCACCGGTAGGCTGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCg                                                                                                           <  3:179189/149‑1 (MQ=255)
                                      cAGCCAGTGCACCGGTAGGCTGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGGAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTg                                                                                                        >  1:317509/1‑149 (MQ=255)
                                       aGCCAGTGCACCGGTAGGCTGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTg                                                                                                        <  5:239410/148‑1 (MQ=255)
                                        gCCAGTGCACCGGTAGGCTGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGcc                                                                                                      >  3:289792/1‑149 (MQ=255)
                                        gCCAGTGCACCGGTAGGCTGGACGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGcc                                                                                                      >  3:272599/1‑149 (MQ=255)
                                         ccAGTGCACCGGTAGGCTGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTc                                                                                                            >  7:319058/1‑142 (MQ=255)
                                         ccAGTGCACCGGTAGGCTGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTc                                                                                                            <  8:319058/142‑1 (MQ=255)
                                                aCCGGTAGGCTGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGAc                                                                                                >  5:269123/1‑147 (MQ=255)
                                                aCCGGTAGGCTGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGAc                                                                                                >  8:192102/1‑147 (MQ=255)
                                                  cggtgcacTGGCTGACGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAgg                                                                                            <  1:171379/142‑1 (MQ=255)
                                                  cGGTAGGCTGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGGAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAgg                                                                                            <  2:317509/149‑1 (MQ=255)
                                                  cGGTAGGCTGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTgg                                                                                                   <  5:286637/142‑1 (MQ=255)
                                                  cGGTAGGCTGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTgg                                                                                                   >  6:286637/1‑142 (MQ=255)
                                                  cGGTAGGCTGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAgg                                                                                            <  7:192102/149‑1 (MQ=255)
                                                  cGGTAGGCTGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAgg                                                                                            <  6:141817/149‑1 (MQ=255)
                                                        gCTGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTc                                                                                      <  1:264348/149‑1 (MQ=255)
                                                          tGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAg                                                                                    >  3:28408/1‑149 (MQ=255)
                                                          tGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAg                                                                                    >  1:141890/1‑149 (MQ=255)
                                                            gccgccgccGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGt                                                                                  >  2:182474/1‑149 (MQ=255)
                                                             ccgccgccgTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGtt                                                                                 <  8:327400/149‑1 (MQ=255)
                                                              cgccgccgTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAg                                                                                    >  3:208537/1‑145 (MQ=255)
                                                              cgccgccgTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAg                                                                                    <  4:208537/145‑1 (MQ=255)
                                                               gccgccgTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTAGGTGCCTGGGACCAGGTTGTTCAGGTTCa                                                                               >  1:205447/1‑149 (MQ=255)
                                                                ccgccgTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCAc                                                                              <  4:329888/149‑1 (MQ=255)
                                                                ccgccgTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCAc                                                                              >  2:358089/1‑149 (MQ=255)
                                                                  gccgTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGa                                                                            <  3:89350/149‑1 (MQ=255)
                                                                              cAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGtgttgt                                                                <  4:28408/149‑1 (MQ=255)
                                                                               aGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGTGTtgtg                                                               >  1:24830/1‑149 (MQ=255)
                                                                                ggCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGTGTTgtgg                                                              <  5:206837/149‑1 (MQ=255)
                                                                                 gctgTTCAAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCACGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCATGTTCACGACATAGGTGTTgtggt                                                             <  6:205494/146‑1 (MQ=255)
                                                                                       ccAGTAGAAGGTGTGGTTCCAGTCTTGAGCGGCGTTGTTGAAGATGCGGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTGGAATTCGGTGCCTGGGACCATGTTGTTCAGGTTCACGACATAGGTGTTGTGGTGCTTg                                                        <  5:95162/148‑1 (MQ=255)
                                                                                           tAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGTGTTGTGGTGCTTGTCgtg                                                   >  3:362326/1‑149 (MQ=255)
                                                                                             gAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGTGTTGTGGTGCTTGTCgtggt                                                 <  4:289792/149‑1 (MQ=255)
                                                                                             gAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGTGTTGTGGTGCTTGTCgtgg                                                  <  6:7306/148‑1 (MQ=255)
                                                                                             gAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGTGTTGTGGTGCTTGTCgtgg                                                  <  8:67226/148‑1 (MQ=255)
                                                                                             gAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGTGTTGTGGTGCTTGTCgtgg                                                  >  3:216008/1‑148 (MQ=255)
                                                                                             gAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGTGTTGTGGTGCTTGTCgtgg                                                  <  4:216008/148‑1 (MQ=255)
                                                                                               aGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGTGTTGTGGTGCTTGTCGTGGTGa                                               <  5:59509/149‑1 (MQ=255)
                                                                                               aGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGTGTTGTGGTGCTTGTCGTGGTGa                                               <  2:24830/149‑1 (MQ=255)
                                                                                                     ggTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGTGTTGTGGTGCTTGTCGTGGTGATACTc                                          >  2:145169/1‑148 (MQ=255)
                                                                                                       ttCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGTGTTGTGGTGCTTGTCGTGGTGa                                               <  2:265985/141‑1 (MQ=255)
                                                                                                       ttCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCATGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGTGTTGTGGTGCTTGTCGTGGTGa                                               >  1:265985/1‑141 (MQ=255)
                                                                                                         ccAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGTGTTGTGGTGCTTGTCGTGGTGATACTCCAggg                                     >  3:175401/1‑149 (MQ=255)
                                                                                                         ccAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGTGTTGTGGTGCTTGTCGTGGTGATACTCCAgg                                      <  4:346731/148‑1 (MQ=255)
                                                                                                         ccAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGTGTTGTGGTGCTTGTCGTGGTGATAATCAAggg                                     >  4:25937/1‑149 (MQ=255)
                                                                                                          cAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGTGTTGTGGTGCTTGTCGTGGTGATACTCCAGGGt                                    >  2:304639/1‑149 (MQ=255)
                                                                                                           aGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGTGTTGTGGTGCTTGTCGTGGTGATACTCCAGGGtt                                   <  6:269123/149‑1 (MQ=255)
                                                                                                               ttGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGTGTTGTGGTGCTTGTCGTGGTGATACTCCAGGGTTTCCt                               >  5:241312/1‑149 (MQ=255)
                                                                                                                 gAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGTGTTGTGGTGCTTGTCgtggt                                                 <  3:133333/129‑1 (MQ=255)
                                                                                                                 gAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGTGTTGTGGTGCTTGTCgtggt                                                 >  4:133333/1‑129 (MQ=255)
                                                                                                                   gcggcgTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGTGTTGTGGTGCTTGTCGTGGTGATACTCCAGGGTTTCCTTGga                           <  4:362326/149‑1 (MQ=255)
                                                                                                                                aGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGTGTTGTGGTGCTTGTCGTGGTGATACTCCAGGGTTTCCTTGGAGATGTGCGGCTGc              <  1:358089/149‑1 (MQ=255)
                                                                                                                                 gATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGTGTTGTGGTGCTTGTCGTGGTGATACTCCAGGGTTTCCTTGGAGATGTGCGGCTGCa             <  2:321196/149‑1 (MQ=255)
                                                                                                                                  aTGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGTGTTGTGGTGCTTGTCGTGGTGATACTCCAGGGTTTCCTTGGAGATGTGCGGCTGCAg            <  3:200028/149‑1 (MQ=255)
                                                                                                                                    gccgccCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGTGTTGTGGTGCTTGTCGTGGTGATACTCCAGGGTTTCCTTGGAGATGTGCGGCTGCAgg           <  6:227986/148‑1 (MQ=255)
                                                                                                                                    gccgccCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCCAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGTGTTGTGGTGCTTGTCGTGGTGATACTCCAGGGTTTCCTTGGAGATGTGCGGCTGCAggg          >  1:265303/1‑149 (MQ=255)
                                                                                                                                     ccgccCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGTGTTGTGGTGCTTGTCGTGGTGATACTCCAGGGTTTCCTTGGAGATGTGCGGCTGCAGGGc         <  3:173054/149‑1 (MQ=255)
                                                                                                                                       gccCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGTGTTGTGGTGCTTGTCGTGGTGATACTCCAGGGTTTCCTTGGAGATGTGCGGCTGCAGGGcat       >  1:36797/1‑149 (MQ=255)
                                                                                                                                        ctggAAGAGATCGTCAAGAGCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGTGTTGTGGTGCTTGTCgtggt                                                 <  6:314191/103‑1 (MQ=255)
                                                                                                                                        ctggAAGAGATCGTCAAGAGCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGTGTTGTGGTGCTTGTCgtggt                                                 >  5:314191/4‑106 (MQ=255)
                                                                                                                                        ctggAAGAGATCGTCAAGAGCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGTGTTGTGGTGCTTGTCGTGGTGATACTCCAGGGTTTCCTTGGAGATGTGCGGCTGCAGGGcat       >  3:79897/4‑148 (MQ=255)
                                                                                                                                        cccGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGTGTTGTGGTGCTTGTCGTGGTGATACTCCAGTGTTTCCTTGGAGATGTGCGGCTGCAGGGcatc      <  3:25937/149‑1 (MQ=255)
                                                                                                                                        cccGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGTGTTGTGGTGCTTGTCGTGGTGATACTCCAGGGTTTCCTTGGAGATGTGCGGCTGCAGGGcat       >  4:6497/1‑148 (MQ=255)
                                                                                                                                         tggAAGAGATCGTCAAGAGCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGTGTTGTGGTGCTTGTCGTGGTGATACTCCAGGGTTTCCTTGGAGATGTGCGGCTGCAGGGcatca     >  4:82374/3‑149 (MQ=255)
                                                                                                                                            ccccgCTCTTGCCGATCTCTTCCAGGGGCTTGCCTGCGAATGCGGTGCCTGGGCCCAGGGTGTTCAGGGTCACGACATAGGTGTTGTGGTGCTTGTCGTGGTGATACTCCAGGGTTTCCTTGGTGATGTGCGGCTGCAGGGCATCATgg  <  7:339313/145‑1 (MQ=255)
                                                                                                                                            aaGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGTGTTGTGGTGCTTGTCGTGGTGATACTCCAGGGTTTCCTTGGAGATGTGCGGCTGCAGGGCATCATgg  <  1:332911/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                            
GAACTTGTCGAAGGAACCGAAAGCGGCGTTGATGGCGTCAGCCAGTGCACCGGTAGGCTGGCCGCCGCCGTTTGGCGACAGGCAGTTCCAGTAGAAGGTGTGGTTCCAGACTTGAGCGGCGTTGTTGAAGATGCCGCCCGAAGAGCTCTTGACGATCTCTTCCAGGGTCTTGCCTTCGAATTCGGTGCCTGGGACCAGGTTGTTCAGGTTCACGACATAGGTGTTGTGGTGCTTGTCGTGGTGATACTCCAGGGTTTCCTTGGAGATGTGCGGCTGCAGGGCATCATGG  >  NC_002947/1058567‑1058855

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑