Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,109,225 G→T 5.7% L10I (CTC→ATC)  PP_0968 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,109,2250GT 5.7% 216.6 / 2.1 70L10I (CTC→ATC) PP_0968hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (29/37);  new base T (2/2);  total (31/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.95e-01

TGCAATTGCACGCCGTCAAGGCCGATGAGCAAGGTTTTAGGGCGGCTCGGGTGCCGGGCCGGGTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCAAACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGTTGCAACAAAGTGGCATGCCCACAACTGGCAGAAATACCAGGTGGTTTACCTCTTTGCCTGCAATCCAGTAAGCTGCCTCAAT‑TCGTCGGATGATTGGTTGAGTGGCATGAATACAGAGCTGACCAAGCGCTCCCTGGTGGAGTT  >  NC_002947/1109077‑1109372
                                                                                                                                                    |                                                                                                                                                    
tgcAATTGCACGCCGTCAAGGCCGATGAGCAAGGTTTTAGGGCGGCTCGGGTGCCGGGCCGGGTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCAAACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGAg                                                                                                                                                      >  8:179322/1‑149 (MQ=255)
tgcAATTGCACGCCGTCAAGGCCGATGAGCAAGGTTTTAGGGCGGCTCGGGTGCCGGGCCGGGTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCAAACTTGCCAGTCGCATGGGGACGCATCCTTGAAAGCACGCAACGAg                                                                                                                                                      >  5:64473/1‑149 (MQ=255)
    aTTGCACGCCGTCAAGGCCGATGAGCAAGGTTTTAGGGCGGCTCGGGTGCCGGGCCGGGTCACAGCCCGGCTGCAGGGCCAGTAACAGCAATAACAGCAAACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGtt                                                                                                                                                  <  7:35424/149‑1 (MQ=255)
       gCACGCCGTCAAGGCCGATGAGCAAGGTTTTAGGGCGGCTCGGGTGCCGGGCCGGGTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCAAACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGTTGc                                                                                                                                                <  4:71945/148‑1 (MQ=255)
        cACGCCGTCAAGGCCGATGAGCAAGGTTTTAGGGCGGCTCGGGTGCCGGGCCGGGTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCAAACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGTTGca                                                                                                                                               <  5:31812/148‑1 (MQ=255)
         aCGCCGTCAAGGCCGATGAGCAAGGTTTTAGGGCGGCTCGGGTGCCGGGCCGGGTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCAAACTTGCCAGTCGCATGGGGTCGCATCATTGAAAGCACGCAACGAGCGTTGcaa                                                                                                                                              >  2:144228/1‑148 (MQ=255)
         aCGCCGTCAAGGCCGATGAGCAAGGTTTTAGGGCGGCTCGGGTGCCGGGCCCGGTCACAGCCCGGCTGCAGGGCCAGGAACAGAAATAACAGCAAACTAGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGTTGcaa                                                                                                                                              >  5:306437/1‑148 (MQ=255)
           gCCGTCAAGGCCGATGAGCAAGGTTTTAGGGCGGCTCGGGTGCCGGGCCGGGTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCAAACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGTTGcaaca                                                                                                                                            >  4:32878/1‑148 (MQ=255)
              gTCAAGGCCGATGAGCAAGGTTTTAGGGCGGCTCGGGTGCCGGGCCGGGTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCAAACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGTTGCAACAAAGt                                                                                                                                        <  3:128484/149‑1 (MQ=255)
                   ggCCGATGAGCAAGGTTTTAGGGCGGCTCGGGTGCCGGGCCGGGTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCAAACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGTTGCAACAAAGTGGCAt                                                                                                                                   <  3:32878/149‑1 (MQ=255)
                           aGCAAGGTTTTAGGGCGGCTCGGGTGCCGGGCCGGGTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCAAACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGTTGCAACAAAGTGGCATGCCCACaa                                                                                                                           <  5:63014/149‑1 (MQ=255)
                            gCAAGGTTTTAGGGCGGCTCGGGTGCCGGGCCGGGTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCAAACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGTTGCAACAAAGTGGCATGCCCACAAc                                                                                                                          >  2:14173/1‑149 (MQ=255)
                            gCAAGGTTTTAGGGCGGCTCGGGTGCCGGGCCGGGTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCAAACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGTTGCAACAAAGTGGCATGCCCACAAc                                                                                                                          >  1:260949/1‑149 (MQ=255)
                             cAAGGTTTTAGGGCGGCTCGGGTGCCGGGCCGGGTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCAAACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGTTGCAACAAAGTg                                                                                                                                       >  7:114363/1‑135 (MQ=255)
                             cAAGGTTTTAGGGCGGCTCGGGTGCCGGGCCGGGTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCAAACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGTTGCAACAAAGTg                                                                                                                                       <  8:114363/135‑1 (MQ=255)
                             cAAGGTTTTAGGGCGGCTCGGGTGCCGGGCCGGGTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCAAACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGTTGCAACAAAGTGGCAt                                                                                                                                   <  6:383482/139‑1 (MQ=255)
                             cAAGGTTTTAGGGCGGCTCGGGTGCCGGGCCGGGTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCAAACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGTTGCAACAAAGTGGCAt                                                                                                                                   >  5:383482/1‑139 (MQ=255)
                               aGGTTTTAGGGCGGCTCGGGTGCCGGGCCGGGTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCAAACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGCTCGTTGcgt                                                                                                                                              >  6:256619/1‑124 (MQ=255)
                               aGGTTTTAGGGCGGCTCGGGTGCCGGGCCGGGTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCAAACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGCTCGTTGcgt                                                                                                                                              <  5:256619/126‑3 (MQ=255)
                                      aGGGCGGCTCGGGTGCCGGGCCGGGTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCAAACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGTTGCAACAAAGTGGCATGCCCACAACTGGCAGaaa                                                                                                                 <  2:260949/148‑1 (MQ=255)
                                       gggCGGCTCGGGTGCCGGGCCGGGTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCAACCTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGTGGCAACAAAGTGGCATGCCCACAACTGGCAGAAATa                                                                                                               >  4:54467/1‑149 (MQ=255)
                                         gcggcTCGGGTGCCGGGCCGGGTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCAAACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGTTGCAACAAAGTGGCATGCCCACAACTGGCAGAAATAc                                                                                                              >  5:346225/1‑148 (MQ=255)
                                         gcggcTCGGGTGCCGGGCCGGGTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCAAACTTGCCAGTCGAATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGTTGCAACAAAGTGGCATGCCCACAACTGGCAGAAATAc                                                                                                              >  3:333673/1‑148 (MQ=255)
                                          cggcTCGGGTGCCGGGCCGGGTCACAGCCCTGCTGCAGGGCCAGGAACAGCAATAACAGCAAACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGTTGCAACAAAGTGGCATGCCCACAACTGGCAGAAATACCa                                                                                                            <  6:278141/149‑1 (MQ=255)
                                           ggcTCGGGTGCCGGGCCGGGTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCAAACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGTTGCAACAAAGTGGCATGCCCACAACTGGCAGAAATACCAg                                                                                                           <  2:28765/149‑1 (MQ=255)
                                           ggcTCGGGTGCCGGGCCGGGTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCAAACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGTTGCAACAAAGTGGCATGCCCACAACTGGCAGAAATACCAg                                                                                                           <  5:135142/149‑1 (MQ=255)
                                            gcTCGGGTGCCGGGCCGGGTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCAAACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGTTGCAACAAAGTGGCATGCCCACAACTGGCAGAAATACCAgg                                                                                                          <  3:129912/149‑1 (MQ=255)
                                                gggTGCCGGGCCGGGTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCAAACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGTTGCAACAAAGTGGCATGCCCACAACTGGCAGAAATACCAggtg                                                                                                        <  3:249395/147‑1 (MQ=255)
                                                       gggccgggTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCAAACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGTTGCAACAAAGTGGCATGCCCACAACTGGCAGAAATACCAGGTGGTTTACctc                                                                                               >  1:148104/1‑149 (MQ=255)
                                                        ggccgggTCAGAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCAAACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGTTGCAACAAAGTGGCATGCCCACAACTGGCAGAAATACCAGGTGGTTTACctct                                                                                              <  3:271640/149‑1 (MQ=255)
                                                               tCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCAAACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGTTGCAACAAAGTGGCATGCCCACAACTGGCAGAAATACCAGGTGGGTTCGCTCTTTGCCTg                                                                                       >  7:332849/1‑149 (MQ=255)
                                                                  caGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCAAACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGTTGCAACAAAGTGGCATGCCCACAACTGGCAGAAATACCAGGTGGTTTACCTCTTTGCCTGCaa                                                                                    <  1:14173/149‑1 (MQ=255)
                                                                   aGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCAAACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGTTGCAACAAAGTGGCATGCCCACAACTGGCAGAAATACCAGGTGGTTTACCTCTTTGCCTGCAAt                                                                                   >  6:78096/1‑149 (MQ=255)
                                                                   aGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCAAACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGTTGCAACAAAGTGGCATGCCCACAACTGGCAGAAATACCAGGTGGTTTACCTCTTTGCCTGCAAt                                                                                   >  8:324670/1‑149 (MQ=255)
                                                                     cccGGCTGCAGGGCCAGGAACAGCAATAACAGCAAACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCTACGAGCGTTGCAACAAAGTGGCATGCCCACAACTGGCAGAAATACCAGGTGGTTTACCTCTTTGCCTGCAATc                                                                                  >  8:177995/1‑148 (MQ=255)
                                                                     cccGGCTGCAGGGCCAGGAACAGCAATAACAGCAAACTTGCCAGTCGCATGGGCTCGCATCCTTGAAAGCACGCAACGAGCGTTGCAACAAAGTGGCATGCCCACAACTGGCAGAAATACCAGGTGGTTTACCTCTTTGCCTGCAATc                                                                                  >  2:221339/1‑148 (MQ=255)
                                                                             cAGGGCCAGGAACAGCAATAACAGCAAACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACTCAACGAGCGTTGCAACAAAGTGGCATGCCCACAACTGGCAGAAATACCAGGTGGTTTACCTCTTTGCCTGCAATCCAGTAAGc                                                                          <  5:280300/148‑1 (MQ=255)
                                                                               gggCCAGGAACAGCAATAACAGCAAACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGTTGCAACAAAGTGGCATGCCCACAACTGGCAGAAATACCAGGTGGTTTACCTCTTTGcc                                                                                         <  8:180009/131‑1 (MQ=255)
                                                                               gggCCAGGAACAGCAATAACAGCAAACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGTTGCAACAAAGTGGCATGCCCACAACTGGCAGAAATACCAGGTGGTTTACCTCTTTGcc                                                                                         >  7:180009/1‑131 (MQ=255)
                                                                                 gCCAGGAACAGCAATAACAGCAAACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGTTGCAACAAAGTGGCATGCCCACAACTGGCAGAAATACCAGGTGGTTTACCTCTTTGCCTGCAATCCAGTAAGCTGCCt                                                                     <  2:148104/149‑1 (MQ=255)
                                                                                    aGGAACAGCAATAACAGCAAACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGTTGCAACAAAGTGGCATGCCCACAACTGGCAGAAATACCAGGTGGTTTACCTCTTTGCCTGCAATCCAGTAAGCTGCCTCaa                                                                  >  4:192850/1‑149 (MQ=255)
                                                                                        aCAGCAATAACAGCAAACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGTTGCAACAAAGTGGCATGCCAACAACTGGCAGAAATACCAGGTGGTTTACCTCTTTGCCTGCAATCCAGTAATCTGCCTCAAT‑tcg                                                             >  5:100227/1‑149 (MQ=255)
                                                                                          aGCAATAACAGCAAACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGTTGCAACAAAGTGGCATGCCCACAACTGGCAGAAATACCAGGTGGTTTACCTCTTTGCCTGCAATCCAGTAAGCTGCCTCAAT‑tcgtc                                                           <  3:192850/149‑1 (MQ=255)
                                                                                                   aGCAAACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGTTGCAACAAAGTGGCATGCCCACAACTGGCAGAAATACCAGGTGGTTTACCTCTTTGCCTGCAATCCAGTAAGCTGCCTCAAT‑TCGTCGGATGATTg                                                  >  4:86182/1‑149 (MQ=255)
                                                                                                      aaaCTTGCCAGTCGCATTGGGTCGCATCCTTGAAAGCACGCAACGAGCGTTGCAACAAAGTGGCATGCCCACAACTGGCAGAAATACCAGGTGGTTTACCTCTTTGCCTGCAATCCAGTAAGCTGCCTCAAT‑TCGTCGGATGATTGGtt                                               <  6:310536/149‑1 (MQ=255)
                                                                                                            gCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGTTGCAACAAAGTGGCATGCCCACAACTGGCAGAAATACCAGGTGGTTTACCTCTTTGCCTGCAATCCAGTAAGCTGCCTCAAT‑TCGTCGGATGATTGGTTGAGt                                           <  6:298251/147‑1 (MQ=255)
                                                                                                              cAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGTTGCAACAAAGTGGCATGCCCACAACTGGCAGAAATACCAGGTGGTTTACCTCTTTGCCTGCAATCCAGTAAGCTGCCTCAAT‑TCGTCGGATGATTGGTTGAGTGGCa                                       >  7:124318/1‑149 (MQ=255)
                                                                                                              cAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGTTGCAACAAAGTGGCATGCCCACAACTGGCAGAAATACCAGGTGGTTTACCTCTTTGCCTGCAATCCAGTAAGCTGCCTCAAT‑TCGTCGGATGATTGGTTGAGTGGCa                                       <  2:159214/149‑1 (MQ=255)
                                                                                                              cAGTCGAATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGTTGCAACAAAGTGGCATGCCCACAACTGGCAGAAATACCAGGTGGTTTACCTCTTTGCCTGCAAACCAGTAAGCTGCCTCAAT‑TCGTCGGATGATTGGTTGAGTGGCa                                       <  4:333673/149‑1 (MQ=255)
                                                                                                                   gCATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGTTGCAACAAAGTGGCATGCCCACAACTGGCAGAAATACCAGGTGGTTTACCTCTTTGCCTGCAATCCAGTAAGCTGCCTCAATATCGTCGGATGATTGGTTGAGTGGCATGaa                                   <  1:221339/149‑1 (MQ=255)
                                                                                                                    cATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGTTGCAACAAAGTGGCATGCCCACAACTGGCAGAAATACCAGGTGGTTTACCTCTTTGCCTGCAATCCAGTAAGCTGCCTCAAT‑TCGTCGGATGATTGGTTGAGTGGCATGAATa                                 >  6:248681/1‑149 (MQ=255)
                                                                                                                    cATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGTTGCAACAAAGTGGCATGCCCACAACTGGCAGAAATACCAGGTGGTTTACCTCTTTGCCTGCAATCCAGTAAGCTGCCTCAAT‑TCGTCGGATGATTGGTTGAGTGGCATGAATa                                 >  7:296362/1‑149 (MQ=255)
                                                                                                                     attgggTCGCATCCTTGAAAGCACGCAACGAGCGTTGCAACAAAGTGGCATGCCCACAACTGGCAGAAATACCAGGTGGTTTACCTCTTTGCCTGCAATCCAGTAAGCTGCCTCAAT‑TCGTCGGATGATTGGTTGAGTGGCATGAATa                                 <  5:248681/145‑1 (MQ=255)
                                                                                                                     aTGGGGTCGCATCCTTGAAAGCACGAAACGAGCGTTGCAACAAAGTGGCATGCCCACAACTGGCAGAAATACCAGGTGGTTTACCTCTTTGCCTGCAATCCAGTAAGCTGCCTCAAT‑TCGTCGGATGATTGGGTGAGTGGCATGagtc                                 >  8:284451/1‑145 (MQ=255)
                                                                                                                           tCGCATCCTTGAAAGCACGCAACGAGCGTTGCAACAAAGTGGCATGCCCACAACTGGCAGAAATACCAGGTCGTTTACCTCTTTGCCTGCAATCCAGTAAGCTGCCTCAAT‑TCGTCGGATGATTGGTTGAGTGGCATGAATACAGAGCt                          <  6:306437/149‑1 (MQ=255)
                                                                                                                             gCATCCTTGAAAGCACGCAACGAGCGTTGCAACAAAGTGGCATGCCCACAACTGGCAGAAATACCAGGTGGTTTACCTCTTTGCCTGCAATCCAGTAAGCTGCCTCAAT‑TCGTCGGATGATTGGTTGAGTGGCATGAATACAGAGCTGa                        <  6:346225/149‑1 (MQ=255)
                                                                                                                               aTCCTTGAAAGCACGCAACGAGCGTTGCAACAAAGTGGCATGCCCACAACTGGCAGAAATACCAGGTGGTTTACCTCTTTGCCTGCAATCCAGTAAGCTGCCTCAAT‑TCGTCGGATGATTGGTTGAGTGGCATGAATACAGAGCTGAcc                      <  3:86182/149‑1 (MQ=255)
                                                                                                                                   ttGAAAGCACGCAACGAGCGTTGCAACAAAGTGGCATGCCCACAACTGGCAGAAATACCAGGTGGTTTACCTCTTTGCCTGCAATCCAGTAAGCTGCCTCAAT‑TCGTCGGATGATTGGTTGAGTGGCATGAATACAGAGCTGACCAAgc                  >  5:183135/1‑149 (MQ=255)
                                                                                                                                     gAAAGCACGCAACGAGCGTTGCAACAAAGTGGCATGCCCACAACTGGCAGAAATACCAGGTGGTTTACCTCTTTGCCTGCAATCCAGTAAGCTGCCTCAAT‑TCGTCGGATGATTGGTTGAGTGGCATGAATACAGAGCTGACCAAgcgc                >  5:53889/1‑149 (MQ=255)
                                                                                                                                       aaGCACGCAACGAGCGTTGCAACAAAGTGGCATGCCCACAACTGGCAGAAATACCAGGTGGTTTACCTCTTTGCCTGCAATCCAGTAAGCTGCCTCAAT‑TCGTCGga                                                        >  7:220343/1‑107 (MQ=255)
                                                                                                                                       aaGCACGCAACGAGCGTTGCAACAAAGTGGCATGCCCACAACTGGCAGAAATACCAGGTGGTTTACCTCTTTGCCTGCAATCCAGTAAGCTGCCTCAAT‑TCGTCGga                                                        <  8:220343/107‑1 (MQ=255)
                                                                                                                                       aaGCACGCAACGAGCGTTGCAACAAAGTGGCATGCCCACAACTGGCAGAAATACCAGGTGGTTTACCTCTTTGCCTGCAATCCAGTAAGCTGCCTCAAT‑TCGTCGGATGATTGGTTGAGTGGCATGAATACAGAGCTGACCAAGCGCTc              >  5:63395/1‑149 (MQ=255)
                                                                                                                                         gCACGCTACGAGCGTTGCAACAAAGTGGCATGCCCACAACTGGCAGAAATACCAGGTGGTTTACCTCTTTGCCTGCAATCCAGTAAGCTGCCTCAAT‑TCGTCGGATGATTGGTTGAGTGGCATGAATACAGAGCTGACCAAGCGCTccc            <  7:177995/149‑1 (MQ=255)
                                                                                                                                         gCACGCAACGAGCGTTGCAACAAAGTGGCATGCCCACAACTGGCAGAAATACCAGGTGGTTTACCTCTTTGCCTGCAATCCAGTAAGCTGCCTCAAT‑TCGTCGGATGATTGGTTGAGTGGCATGAATACAGAGCTGACCAAGCGCTccc            <  6:63395/149‑1 (MQ=255)
                                                                                                                                         gCACGCAACGAGCGTTGCAACAAAGTGGCATGCCCACAACTGGCAGAAATACCAGGTGGTTTACCTCTTTGCCTGCAATCCAGTAAGCTGCCTCAAT‑TCGTCGGATGATTGGTTGAGTGGCATGAATACAGAGCTGACCAAGCGCTccc            <  8:296362/149‑1 (MQ=255)
                                                                                                                                           ttgCAACGCTCGTTGCAACAAAGTGGCATGCCCACAACTGGCAGAAATACCAGGTGGTTTACCTCTTTGCCTGCAATCCAGTAAGCTGCCTCAAT‑TCGTCGGATGATTGGTTGAGTGGCATGAATACAGAGCTGACCAAGCGCTCCCtg          >  1:70632/3‑149 (MQ=255)
                                                                                                                                           actcAACGAGCGTTGCAACAAAGTGGCATGCCCACAACTGGCAGAAATACCAGGTGGTTTACCTCTTTGCCTGCAATCCAGTAAGCTGCCTCAAT‑TCGTCGGATGATTGGTTGAGTGGCATGAATACAGAGCTGACCAAGCGCTCCCtg          <  7:29013/146‑1 (MQ=255)
                                                                                                                                           aCGCAACGAGCGTTGCAACAAAGTGGCATGCCCACAACTGGCAGAAATACCAGGTGGTTTACCTCTTTGCCTGCAATCCAGTAAGCTGCCTCAAT‑TCGTCGGATGATTGGTTGAGTGGc                                        <  1:141846/119‑1 (MQ=255)
                                                                                                                                           aCGCAACGAGCGTTGCAACAAAGTGGCATGCCCACAACTGGCAGAAATACCAGGTGGTTTACCTCTTTGCCTGCAATCCAGTAAGCTGCCTCAAT‑TCGTCGGATGATTGGTTGAGTGGc                                        >  2:141846/1‑119 (MQ=255)
                                                                                                                                              cAACGCTCGTTGCAACAAAGTGGCATGCCCACAACTGGCAGAAATACCAGGTGGTTTACCTCTTTGCCTGCAATCCAGTAAGCTGCCTCAAT‑TCGTCGGATGATTGGTTGAGTGGCATGAATACAGAGCTGACCAAGCGCTCCCtggtg       <  2:70632/149‑1 (MQ=255)
                                                                                                                                                 cGAGCGTTGCAACAAAGTGGCATGCCCACAACTGGCAGAAATACCAGGTGTTTTACCTCTTTGCCTGCAATCCAGTAAGCTGCCTCAAT‑TCGTCGGATGATTGGTTGAGTGGCATGAATACAGAGCTGACCAAGCGCTCCCTGGTGGAg    <  1:144228/149‑1 (MQ=255)
                                                                                                                                                   aGCGTTGCAACACACTGGCATGCCCACAACTGGCAGAAATACCAGGTGGTTTACCTCTTTGCCTGCAATCCAGTAAGCTGCCTCAAT‑TCGTCGGATGATTGGTTGAGTGGCATGAATACAGAGCTGACCAAGCGCTCCCTGGTGGAGtt  <  2:162952/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TGCAATTGCACGCCGTCAAGGCCGATGAGCAAGGTTTTAGGGCGGCTCGGGTGCCGGGCCGGGTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCAAACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGTTGCAACAAAGTGGCATGCCCACAACTGGCAGAAATACCAGGTGGTTTACCTCTTTGCCTGCAATCCAGTAAGCTGCCTCAAT‑TCGTCGGATGATTGGTTGAGTGGCATGAATACAGAGCTGACCAAGCGCTCCCTGGTGGAGTT  >  NC_002947/1109077‑1109372

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑