Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,111,300	T→C	13.0%	intergenic (+21/‑89)	PP_0970 → / → PP_0971	cyanate MFS transporter/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,111,300	0	T	C	13.0%	91.9 / 4.5	38	intergenic (+21/‑89)	PP_0970/PP_0971	cyanate MFS transporter/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (20/13);  new base C (3/2);  total (23/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCATGACCTGACCGGCGGCTGGACGGCACTGGGTTGGATTTTTGCGGTGCTGGGCGTTGGCGCCGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGACCAGCCCCCAATGAGTGATGCCAACCGCGCCCTGATCACCCGTTTCTACCAGGCTTTCCAGCGCCTGGAT  >  NC_002947/1111153‑1111448                                                                                                                                                    |                                                                                                                                                     gCATGACCTGACCGGCGGCTGGACGGCACTGGGTTGGATTTTTGCGGTGCTGGGCGTTGGCGCCGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTc                                                                                                                                                     >  4:317058/1‑149 (MQ=255)         tgaccGGCGGCTGGACGGCACTGGGTTGGATTTTTGCGGTGCTGGGCGTTGGCGCCGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGtgcc                                                                                                                                             >  4:6219/1‑149 (MQ=255)         tgaccGGCGGCTGGACGGCACTGGGTTGGATTTTTGCGGTGCTGGGCGTTGGCGCCGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGCCCTCTtgg                                                                                                                                              >  3:195582/1‑147 (MQ=255)             cggcggCTGGACGGCACTGGGTTGGATTTTTGCGGTGCTGGGCGTTGGCGCCGTGGTGTTAGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCGGTTTTAGCAGTCCCGTCCTCGtgcctgc                                                                                                                                          >  4:222699/1‑148 (MQ=255)                 ggcTGGACGGCACTGGGTTGGATTTTTGCGGTGCTGGGCGTTGGCGCCGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAaga                                                                                                                                     <  3:6219/149‑1 (MQ=255)                         ggCACTGGGTTGGATTTTTGCGGTGCTGGGCGTTGGCGCCGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGCCCTCTTGGCAGGCACGAGGACGGGa                                                                                                                             <  3:332208/149‑1 (MQ=255)                             cTGGGTTGGATTTTTGCGGTGCTGGGCGTTGGCGCCGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGCCCTCTTGGCAGGCACGAGGACGGGACTgc                                                                                                                         <  4:195582/149‑2 (MQ=38)                              tGGGTTGGATTTTTGCGGTGCTGGGCGTTGGCGCCGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTgg                                                                                                                         >  4:260766/1‑148 (MQ=255)                               gggTTGGATTTTTGCGGTGCTGGGCGTTGGCGCCGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGCCCTCTTGGCAGGCACGAGGACGGGACTgct                                                                                                                        >  5:345352/1‑146 (MQ=38)                                ggTTGGATTTTTGCGGTGCTGGGCGTTGGCGCCGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGc                                                                                                                        <  3:260766/147‑1 (MQ=255)                                  ttGGATTTTTGCGGTGCTGGGCGTTGGCGCCGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCAt                                                                                                                      >  3:188699/1‑147 (MQ=255)                                    ggATTTTTGCGGTGCTGGGCGTTGGCGCCGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAg                                                                                                                   <  1:335155/148‑1 (MQ=255)                                             cGGTGCTGGGCGTTGGCGCCGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGc                                                                                                         <  8:18832/149‑1 (MQ=255)                                                    gggCGTTGGCGCCGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTAt                                                                                                  >  7:194954/1‑149 (MQ=255)                                                     ggCGTTGGCGCGGTGGGGGTGGGGCTGGGGGCGGGGCGGGCGCTGCATGGGCAGGTGACCTGCGAAAACGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATc                                                                                                 <  5:247865/149‑1 (MQ=255)                                                        gTTGGCGCCGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTg                                                                                              >  6:30181/1‑149 (MQ=255)                                                              gcCGTGGTGTTCGGGCTGGGTGCCGAGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGttt                                                                                        >  5:227281/1‑149 (MQ=255)                                                                   ggggtTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCCGCGAAAAAGGCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTTCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGaa                                                                                   <  7:187432/146‑1 (MQ=255)                                                                       ttCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAAcc                                                                                 >  3:274225/1‑147 (MQ=255)                                                                                 gTGCCGTGCGGGCGCGGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGACCa                                                                     <  8:231658/149‑1 (MQ=255)                                                                                                       gCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGACCAGCCCCCAATGAGTGATGCCAAc                                               >  7:226936/1‑149 (MQ=255)                                                                                                              aCCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGACCAGCCCCCAATGAGTGATGCCAAc                                               <  4:39561/142‑1 (MQ=255)                                                                                                              aCCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGACCAGCCCCCAATGAGTGATGCCAAc                                               >  3:39561/1‑142 (MQ=255)                                                                                                                cTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCTAGAGGGCGGGACTGGCATAAGCCACCCGGGCAGATTATCGTGCAGTATTCGAACCTAGGACGGACCAGCCCCCAATGAGTGATGCCCACAGCGCCATg                                      >  4:263043/1‑149 (MQ=255)                                                                                                                cTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGACCAGCCCCCAATGAGTGATGCCAACCGCGCCCt                                       >  5:118179/1‑148 (MQ=255)                                                                                                                cTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGACCAGCCCCCAATGAGTCATGCCAACCGCGCCCt                                       >  4:22464/1‑148 (MQ=255)                                                                                                                cTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGATTTCGAACCTAGGACGGACCAGCCTCCAATGAGTGATGCCAACCGCGCCCt                                       >  6:107432/1‑148 (MQ=255)                                                                                                                   cGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGACCAGCCCCCAATGAGTGATGCCAACCGCGCCCTGATc                                   <  8:194954/149‑1 (MQ=255)                                                                                                                      aaaaGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGACCAGCCCCCAATGAGTGATGCCAACCGCGCCCTGATCAc                                 <  5:30181/148‑1 (MQ=255)                                                                                                                        aaGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGACCAGCCCCCAATGAGTGATGCCAACCGCGCCCTGATCACCCg                              >  6:134315/1‑149 (MQ=255)                                                                                                                             tGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGACCAGCCCCCAATGAGTGATGCCAACCGCGCCCTGATCACCCGTTTc                          >  3:90047/1‑148 (MQ=255)                                                                                                                                 gCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGACCAGCCCCCAATGAGTGATGCCAACCGCGCCCTGATCACCCGTTTCTAcc                      <  2:32989/148‑1 (MQ=255)                                                                                                                                 gCCTGTTTTAGCAGTCCCGTCCTCGTGCCTCCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGACCAGCCCCCAATGAGTGATGCCAACCGCGCCCTGATCACCCGTTTCTAcc                      >  1:32989/1‑148 (MQ=255)                                                                                                                                      ttttAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGACCAGCCCCCAATGAGTGATGCCAACCGCGCCCTGATCACCCGTTTCTACCAGGc                  <  3:326595/147‑1 (MQ=255)                                                                                                                                         tAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGACCAGCCCCCAATGAGTGATGCCAACCGCGCCCTGATCACCCGTTTCTACCAGGCTTTc              <  5:134315/148‑1 (MQ=255)                                                                                                                                           gCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGACCAGCCCCCAATGAGTGATGCCAACCGCGCCCTGATCACCCGTTTCTACCAGGCTTTCCAg           >  3:364938/1‑149 (MQ=255)                                                                                                                                              gTCCCGCCCTCTTGGCAGGCACGAGGACGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGACCAGCCCCCAATGAGTGATGCCAACCGCGCCCTGATCACCCGTTTCTACCAGGCTTTCCAgcgc        >  3:109203/1‑149 (MQ=39)                                                                                                                                               tCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGACCAGCCCCCAATGAGTGATGCCAACCGCGCCCTGATCACCCGTTTCTACCAGGCTTTCCAGCGcc       >  1:232803/1‑149 (MQ=255)                                                                                                                                                    tCCTCGTGCCTTCCTAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGACCAGCCCCCAATGAGTGATGCCAACCGCGCCCTGATCACCCGTTTCTACCAGGCTTTCCAGCGCCTGGAt  <  3:263043/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                     GCATGACCTGACCGGCGGCTGGACGGCACTGGGTTGGATTTTTGCGGTGCTGGGCGTTGGCGCCGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGACCAGCCCCCAATGAGTGATGCCAACCGCGCCCTGATCACCCGTTTCTACCAGGCTTTCCAGCGCCTGGAT  >  NC_002947/1111153‑1111448

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑