Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,116,283	T→C	9.1%	I517V (ATC→GTC)	valS ←	valine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,116,283	0	T	C	9.1%	181.5 / 6.5	66	I517V (ATC→GTC)	valS	valine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base T (29/31);  new base C (3/3);  total (32/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATGTCCAGCGGGTCCAGCACGTTGCCCTTGGACTTGGACATCTTCTGGCCCTGGCCGTCACGTACCAGGCCGTGCACGTACACGGTCTTGAACGGTACCTGCGGGGTGCCGTCTTCGTTCTTGATCAGGTGCATGGTCAGCATGATCATGCGCGCAACCCAGAAGAAGATGATGTCGAAGCCAGTCACCAGCACGTCGGTGGAGTGGAACTTCTTGAGGAATTCGGTCTGCTCCGGCC‑AGCCCAGGGTGGAGAATGTCCACAGGCCCGAGCTGAACCAGGTGTCGAG  >  NC_002947/1116137‑1116424                                                                                                                                                   |                                                                                                                                               gaTGTCCAGCGGGTCCAGCACGTTGCCCTTGGACTTGGACATCTTCTGGCCCTGGCCGTCACGTACCAGGCCGTGCACGTACACGGTCTTGAACGGTACCTGCGGGGTGCCGTCTTCGTTCTTGATCAGGTGCATGGTCAGCATGATCa                                                                                                                                              <  4:166079/149‑1 (MQ=255) gaTGTCCAGCGGGTCCAGCACGTTGCCCTTGGACTTGGACATCTTCTGGCCCTGGCCGTCACGTACCAGGCCGTGCACGTACACGGTCTTGAACGGTACCTGCGGGGTGCCGTCTTCGTTCTTGATCAGGTGCATGGTCAGCATGATCa                                                                                                                                              >  4:185487/1‑149 (MQ=255)     tCCAGCGGGTCCAGCACGTTGCCCTTGGACTTGGACATCTTCTGGCCCTGGCCGTCACGTACCAGGCCGTGCACGTACACGGTCTTGAACGGTACCTGCGGGGTGCCGTCTTCGTTCTTGATCAGGGGCATGGTCAGCATGATCATgcg                                                                                                                                          <  3:317005/149‑1 (MQ=255)       cAGCGGGTCCAGCACGTTGCCCTTGGACTTGGACATCTTCTGGCCCTGGCCGTCACGTACCAGGCCGTGCACGTACACGGTCTTGAACGGTACCTGCGGGGTGCCGTCTTCGTTCTTGATCAGGTGCATGATCATGCTGACCATgcac                                                                                                                                         >  6:350254/1‑146 (MQ=255)       cAGCGGGTCCAGCACGTTGCCCTTGGACTTGGACATCTTCTGGCCCTGGCCGTCACGTACCAGGCCGTGCACGTACACGGTCTTGAACGGTACCTGCGGGGTGCCGTCTTCGTTCTTGATCAGGTGCATGATCATGCTGACCATgcac                                                                                                                                         >  6:344614/1‑146 (MQ=255)       cAGCGGGTCCAGCACGTTGCCCTTGGACTTGGACATCTTCTGGCCCTGGCCGTCACGTACCAGGCCGTGCACGTACACGGTCTTGAACGGTACCTGCGGGGTGCCGTCTTCGTTCTTGATCAGGTGCATGATCATGCTGACCATgcac                                                                                                                                         <  5:350254/148‑3 (MQ=255)       cAGCGGGTCCAGCACGTTGCCCTTGGACTTGGACATCTTCTGGCCCTGGCCGTCACGTACCAGGCCGTGCACGTACACGGTCTTGAACGGTACCTGCGGGGTGCCGTCTTCGTTCTTGATCAGGTGCATGATCATGCTGACCATgcac                                                                                                                                         <  5:344614/148‑3 (MQ=255)       cAGCGGGTCCAGCACGTTGCCCTTGGACTTGGACATCTTCTGGCCCTGGCCGTCACGTACCAGGCCGTGCACGTACACGGTCTTGAACGGTACCTGCGGGGGGCCGTCTTCGTTCTTGATCAGGTGCATGATCATGCTGACCATgcacc                                                                                                                                        <  4:234312/149‑4 (MQ=255)        aGCGGGTCCAGCACGTTGCCCTTGGACTTGGACATCTTCTGGCCCTGGCCGTCACGTACCAGGCCGTGCACGTACACGGTCTTGAACGGTACCTGCGGGGTGCCGTCTTCGTTCTTGATCAGGTGCATGGTCAGCATGATCATgcgcgc                                                                                                                                       <  5:186231/149‑1 (MQ=255)           gggTCCAGCACGTTGCCCTTGGACTTGGACATCTTCTGGCCCTGGCCGTCACGTACCAGGCCGTGCACGTACACGGTCTTGAACGGTACCTGCGGGGTGCCGTCTTCGTTCTTGATCAGGTGCATGGTCAGCATGATCATGCGCGCAAc                                                                                                                                    <  5:330829/149‑1 (MQ=255)            ggTCCAGCACGTTGCCCTTGGACTTGGACATCTTCTGGCCCTGGCCGTCACGTACCAGGCCGTGCACGTACACGGTCTTGAACGGTACCTGCGGGGTGCCGTCTTCGTTCTTGATCAGGCGCATGATCATGCTGACCATgcacctgat                                                                                                                                    >  5:231117/1‑141 (MQ=255)              tCCAGCACGTTGCCCTTGGACTTGGACATCTTCTGGCCCTGGCCGTCACGTACCAGGCCGTGCACGTACACGGTCTTGAACGGTACCTGCGGGGTGCCGTCTTCGTTCTTGATCAGGTGCATGGTCAGCATGATCATGCGCGCAAcc                                                                                                                                   >  3:173518/1‑147 (MQ=255)                 aGCACGTTGCCCTTGGACTTGGACATCTTCTGGCCCTGGCCGTCACGTACCAGGCCGTGCACGTACACGGTCTTGAACGGTACCTGCGGGGTGCCGTCTTCGTTCTTGATCAGGTGCATGGTCAGCATGATCATGCGCGCAACCCagaa                                                                                                                              <  1:53517/149‑1 (MQ=255)                 aGCACGTTGCCCTTGGACTTGGACATCTTCTGGCCCTGGCCGTCACGTACCAGGCCGTGCACGTACACGGTCTTGAACGGTACCTGCGGGGTGCCGTCTTCGTTCTTGATCAGGTGCATGGTCAGCATGATCATGCGCGCAACCCagaa                                                                                                                              <  3:55703/149‑1 (MQ=255)                      gTTGCCCTTGGACTTGGACATCTTCTGGCCCTGGCCGTCACGTACCAGGCCGTGCACGTACACGGTCTTGAACGGTACCTGCGGGGTGCCGTCTTCGTTCTTGATCAGGTGCATGGTCAGCATGATCATGCGCGCAACCCagaag                                                                                                                             >  2:221390/1‑145 (MQ=255)                       ttGCCCTTGGACTTGGACATCTTCTGGCCCTGGCCGTCACGTACCAGGCCGTTCACGTACTCGGTCTTGAACGGTACCTGCGGGGGGCCGTCTTCGTTCTTGATCAGGTGCATGGTCAGCATGATCATGCGCGCAACCCAGAAGAAgat                                                                                                                        <  7:59060/149‑1 (MQ=255)                       ttGCCCTTGGACTTGGACATCTTCTGGCCCTGGCCGTCACGTACCAGGCCGTGCACGTACACGGTCTTGAACGGTACCTGCGGGGTGCCGTCTTCGTTCTTGATCAGGTGCATGGTCAGCATGATCATGCGCGCAACCCAGAAGAAgat                                                                                                                        <  4:270883/149‑1 (MQ=255)                       ttGCCCTTGGACTTGGACATCTTCTGGCCCTGGCCGTCACGTACCAGGCCGTGCACGTACACGGTCTTGAACGGTACCTGCGGGGTGCCGTCTTCGTTCTTGATCAGGTGCATGGTCAGCATGATCATGCGCGCAACCCAGAAGAAgat                                                                                                                        <  1:167005/149‑1 (MQ=255)                       ttGCCCTTGGACTTGGACATCTTCTGGCCCTGGCCGTCACGTACCAGGCCGTGCACGTACACGGTCTTGAACGGTACCTGCGGGGTGCCGTCTTCGTTCTTGATCAGGTGCATGGTCAGCATGATCATGCGCGCAACCCAGAAGAAgat                                                                                                                        <  5:356956/149‑1 (MQ=255)                           ccTTGGACTTGGACATCTTCTGGCCCTGGCCGTCACGTACCAGGCCGTGCACGTACACGGTCTTGAACGGTACCTGCGGGGTGCCGTCTTCGTTCTTGATCAGGTGCATGGTCAGCATGATCATGCGCGCAACCCAGAAGAAgatgat                                                                                                                     <  7:98718/148‑1 (MQ=255)                                     ggacATCTTCTGGCCCTGGCCGTCACGTACCAGGCCGTGCACGTACACGGTCTTGAACGGTACCTGCGGGGTGCCGTCTTCGTTCTTGATCAGGTGCATGGTCAGCATGATCATGCGCGCAACCCAGAAGAAGATGATGTCGAAGCCAg                                                                                                          >  6:144284/1‑149 (MQ=255)                                       acATCTTCTGGCCCTGGCCGTCACGTACCAGGCCGTGCACGTACACGGTCTTGAACGGTACCTGCGGGGTGCCGTCTTCGTTCTTGATCAGGTGCATGGTCAGCATGATCATGCGCGCAACCCAGAAGAAGATGATGTCGAAGCCAGt                                                                                                         >  3:170414/1‑148 (MQ=255)                                         aTCTTCTGGCCCTGGCCGTCACGTACCAGGCCGTGCACGTACACGGTCTTGAACGGTACCTGCGGGGTGCCGTCTTCGTTCTTGATCAGGTGCATGGTCAGCATGATCATGCGCGCAACCCAGAAGAAGATGATGTCGAAGCCAGTCAc                                                                                                      <  6:238833/149‑1 (MQ=255)                                                 gccctggccGTCACGTACCAGGCCGTGCACGTACACGGTCTTGAACGGTACCTGCGGGGTGCCGTCTTCGTTCTTGATCAGGTGCATGGTCAGCATGATCATGCGCGCAACCCAGAAGAAGATGATGTCGAAGCCAGTCACCAGCACgc                                                                                              >  5:3087/1‑148 (MQ=255)                                                   cctggccGTCACGTACCAGGCCGTGCACGTACACGGTCTTGAACGGTACCTGCGGGGTGCCGTCTTCGTTCTTGATCAGGTGCATGGTCAGCATGATCATGCGCGCAACCCAGAAGAAGATGATGTCGAAGc                                                                                                             >  6:264844/1‑132 (MQ=255)                                                   cctggccGTCACGTACCAGGCCGTGCACGTACACGGTCTTGAACGGTACCTGCGGGGTGCCGTCTTCGTTCTTGATCAGGTGCATGGTCAGCATGATCATGCGCGCAACCCAGAAGAAGATGATGTCGAAGc                                                                                                             <  5:264844/132‑1 (MQ=255)                                                     tggccGTCACGTACCAGGCCGTGCACGTACACGGTCTTGAACGGTACCTGCGGGGTGCCGTCTTCGTTCTTGATCAGGTGCATGGTCAGCATGATCATGCGCGCAACGCAGAAGAAGATGATGTCGAAGCCAGTCACCAGCACGTCGgt                                                                                          >  5:321891/1‑149 (MQ=255)                                                       gccGTCACGTACCAGGCCGTGCACGTACACGGTCTTGAACGGTACCTGCGGGGTGCCGTCTTCGTTCTTGATCAGGTGCATGGTCAGCATGATCATGCGCGCAACCCAGAAGAAGATGATGTCGAAGCCAGTCACCAGCACGTCGgtg                                                                                         <  2:83314/148‑1 (MQ=255)                                                           tCACGTACCAGGCCGTGCACGTACACGGTCTTGAACGGTACCTGCGGGGTGCCGTCTTCGTTCTTGATCAGGTGCATGGTCAGCATGATCATGCGCGCAACCCAGAAGAAGATGATGTCGAAGCCAGTCACCAGCACGTCGgtggagtg                                                                                    >  8:131406/1‑149 (MQ=255)                                                            cACGTACCAGGCCGTGCACGTACACGGTCTTGAACGGTACCTGCGGGGTGCCGTCTTCGTTCTTGATCAGGTGCATGGTCAGCATGATCATGCGCGCAACCCAGAAGAAGATGATGTCGAAGCCAGTCACCAGCACGTCGgtggagtg                                                                                    >  7:49056/1‑148 (MQ=255)                                                                    aGGCCGTGCACGTACACGGTCTTGAACGGTAGCTGCGGGGTGCCGTCTTCGTTCTTGATCAGGTGCATGGTCAGCATGATCATGCGCGCAACCCAGAAGAAGATGATGTCGAAGCCAGTCACCAGCACGTCGGTGGAGTGGAActtctt                                                                           <  4:170414/149‑1 (MQ=255)                                                                     ggCCGTGCACGTACACGGTCTTGAACGGTACCTGCGGGGTGCCGTCTTCGTTCTTGATCAGGTGCATGGTCAGCATGATCATGCGCGCAACCCAGAAGAAGATGATGTCGAAGCCAGTCAc                                                                                                      >  2:338519/1‑121 (MQ=255)                                                                     ggCCGTGCACGTACACGGTCTTGAACGGTACCTGCGGGGTGCCGTCTTCGTTCTTGATCAGGTGCATGGTCAGCATGATCATGCGCGCAACCCAGAAGAAGATGATGTCGAAGCCAGTCAc                                                                                                      <  1:338519/121‑1 (MQ=255)                                                                      gCCGTGCACGTACACGGTCTTGAACGGTACCTGCGGGGTGCCGTCTTCGTTCTTGATCAGGTGCATGGTCAGCATGATCATGCGCGCAACCCAGAAGAAGATGATGTCGAAGCCAGTCACCAGCACGTCGGTGGAGTGGAACTTCTTGa                                                                         <  2:75239/149‑1 (MQ=255)                                                                              cGTACACGGTCTTGAACGGTACCTGCGGGGTGCCGTCTTCGTTCTTGATCAGGTGCATGGTCAGCATGATCATGCGCGCAACCCAGAAGAAGATGATGTCGAAGCCAGTCACCAGCACGTCGGTGGAGTGGAACTTCTTGAGGAATTCg                                                                 <  7:131406/149‑1 (MQ=255)                                                                                  cacGGTCTTGAACGGTACCTGCGGGGTGCCGTCTTCGTTCTTGATCAGGTGCATGGTCAGCATGATCATGCGCGCAACCCAGAAGAAGATGATGTCGAAGCCAGTCACCAGCACGTCGGTGGAGTGGAACTTCTTGAGGAATTCGGTCt                                                             >  2:148330/1‑149 (MQ=255)                                                                                  cacGGTCTTGAACGGTACCTGCGGGGTGCCGTCTTCGTTCTTGATCAGGTGCATGGTCAGCATGATCATGCGCGCAACCCAGAAGAAGATGATGTCGAAGCCAGTCACCAGCACGCCGGTGGAGTGGAACTTCTTGAGGAATTCGGTc                                                              <  6:3087/148‑1 (MQ=255)                                                                                                        ggggTGCCGTCTTCGTTCTTGATCAGGTGCATGGTCAGCATGATCATGCGCGCAACCCAGAAGAAGATGATGTCGAAGCCAGTCACCAGCACGTCGGTGGAGTGGAACTTCTTGAGGAATTCGGTCTGCTCCGGCC‑AGCCCAGGGTGga                                      <  3:185487/149‑1 (MQ=255)                                                                                                            tGCCGTCTTCGTTCTTGATCAGGTGCATGGTCAGCATGATCATGCGCGCAACCCAGAAGAAGATGATGTCGAAGCCAGTCACCAGCACGTCGGTGGAGTGGAACTTCTTGAGGAATTCGGTCTGCTCCGGCC‑AGCCCAGGGTGGAGAAt                                  <  2:11303/149‑1 (MQ=255)                                                                                                              ccGTCTTCGTTCTTGATCAGGTGCATGGTCAGCATGATCATGCGCGCAACCCAGAAGAAGATGATGTCGAAGCCAGTCACCAGCACGTCGGTGGAGTGGAACTTCTTGAGGAATTCGGTCTGCTCCGGCC‑AGCCCAGGGTGGAGAATGt                                >  1:200378/1‑149 (MQ=255)                                                                                                              ccGTCTTCGTTCTTGATCAGGTGCATGGTCAGCATGATCATGCGCGCAACCCAGAAGAAGATGATGTCGAAGCCAGTCACCAGCACGTCGGTGGAGTGGAACTTCTTGAGGAATTCGGTCTGCTCCGGCC‑AGCCCAGGGTGGAGAATGt                                >  2:35162/1‑149 (MQ=255)                                                                                                                gTCTTCGTTCTTGATCAGGTGCATGGTCAGCATGATCATGCGCGCAACCCAGAAGAAGATGATGTCGAAGCCAGTCACCAGCACGTCGGTGGAGTGGAACTTCTTGAGGAATTCGGTCTGCTCCGGCC‑AGCCCAGGGTGGAGAATGTc                               >  3:247172/1‑148 (MQ=255)                                                                                                                gTCTTCGTTCTTGATCAGGTGCATGGTCAGCATGATCATGCGCGCAACCCAGAAGAAGATGATGTCGAAGCCAGTCACCAGCACGTCGGTGGAGTGGAACTTCTTGAGGAATTCGGTCTGCTCCGGCC‑AGCCCAGGGTGGAGAATGTc                               >  8:319894/1‑148 (MQ=255)                                                                                                                 tCTTCGTTCTTGATCAGGTGCATGGTCAGCATGATCATGCGCGCAACCCAGAAGAAGATGATGTCGAAGCCAGTCACCAGCACGTCGGTGGAGTGGAACTTCTTGAGGAATTCGGTCTGCTCCGGCC‑AGCCCAGGGTGGAGAATGTCca                             >  6:219412/1‑149 (MQ=255)                                                                                                                 tCTTCGTTCTTGATCAGGTGCATGGTCAGCATGATCATGCGCGCAACCCAGAAGAAGATGATGTCGAAGCCAGTCACCAGCACGTCGGTGGAGTGGAACTTCTTGAGGAATTCGGTCTGCTCCGGCC‑AGCCCAGGGTGGAGAATGTCca                             >  1:282284/1‑149 (MQ=255)                                                                                                                 tCTTCGTTCTTGATCAGGTGCATGGTCAGCATGATCATGCGCGCAACCCAGAAGAAGATGATGTCGAAGCCAGTCACCAGCACGTCGGTGGAGTGGAACTTCTTGAGGAATTCGGTCTGCTCCGGCC‑AGCCCAGGGTGGAGAATGTCca                             >  2:47259/1‑149 (MQ=255)                                                                                                                     cGTTCTTGATCAGGTGCATGGTCAGCATGATCATGCGCGCAACCCAGAAGAATATGATGGCGAAGCCAGTCACCAGCACGTCGGTGGAGTGGAACTTCTTGAGGAATTCGGTCTGCTCCGGCC‑AGCCCAGGGTGGAGAATGTCcaca                           <  7:274781/147‑1 (MQ=255)                                                                                                                       ttCTTGATCAGGTGCATGGTCAGCATGATCATGCGCGCAACCCAGAAGAAGATGATGTCGAAGCCAGTCACCAGCACGTCGGTGGAGTGGAACTTCTTGAGGAATTCGGTCTGCTCGGGCC‑AGCCCAGGGTGGAGAATGTCCACAGGcc                       >  4:85967/1‑149 (MQ=255)                                                                                                                         cTTGATCAGGTGCATGGTCAGCATGATCATGCGCGCAACCCAGAAGAAGATGATGTCGAAGCCAGTCACCAGCACGTCGGTGGAGTGGAACTTCTTGAGGAATTCGGTCTGCTCCGGCC‑AGCCCAGGGTGGAGAATGTCCACAGGccc                      <  4:173518/148‑1 (MQ=255)                                                                                                                         cTTGATCAGGTGCATGGTCAGCATGATCATGCGCGCAACCCAGAAGAAGATGATGTCGAAGCCAGTCACCAGCACGTCGGTGGAGTGGAACTTCTTGAGGAATTCGGTCTGCTCCGGCC‑AGCCCAGGGTGGAGAATGTCCACAGGccc                      <  1:47259/148‑1 (MQ=255)                                                                                                                          ttGATCAGGTGCATGGTCAGCATGATCATGCGCGCAACCCAGAAGAAGATGGTGTCGAAGCCAGTCACCAGCACGTCGGTGGAGTGGAACTTCTTGAGGAATTCGGTCTGCTCCGGCC‑AGCCCAGGGTGGAGAATGTCCACAGGccc                      >  7:8977/1‑147 (MQ=255)                                                                                                                          ttGATCAGGTGCATGGTCAGCATGATCATGCGCGCAACCCAGAAGAAGATGATGTCGAAGCCAGTCACCAGCACGTCGGTGGAGTGGAACTTCTTGAGGAATTCGGTCTGCTCCGGCC‑AGCCCAGGGTGGAGAATGTCCACAGGccc                      >  8:140008/1‑147 (MQ=255)                                                                                                                            gATCAGGTGCATGGTCAGCATGATCATGCGCGCAACCCAGAAGAAGATGATGTCGAATCCAGTCACCAGCACGTCGGTGGAGTGGAACTTCTTGAGGAATTCGGTCTGCTCCGGCCAAGCCCAGGGTGGAGAATGTCCACAGTccc                      <  1:221390/146‑1 (MQ=255)                                                                                                                            gATCAGGTGCATGGTCAGCATGATCATGCGCGCAACCCAGAAGAAGATGATGTCGAAGCCAGTCACCAGCACGTCGGTGGAGTGGAACTTCTTGAGGAATTCGGTCTGCTCCGGCC‑AGCCCAGGGTGGAGAATGTCCACAGGCCCGAg                   <  1:148330/148‑1 (MQ=255)                                                                                                                                   tGCATGGTCAGCATGATCATGCGCGCAACCCAGAAGAAGATGATGTCGAAGCCAGTCACCAGCACGTCGGTGGAGTGGAACTTCTTGAGGAATTCGGTCTGCTCCGGCC‑AGCCCAGGGTGgaga                                    <  3:219024/124‑1 (MQ=255)                                                                                                                                   tGCATGGTCAGCATGATCATGCGCGCAACCCAGAAGAAGATGATGTCGAAGCCAGTCACCAGCACGTCGGTGGAGTGGAACTTCTTGAGGAATTCGGTCTGCTCCGGCC‑AGCCCAGGGTGgaga                                    >  4:219024/1‑124 (MQ=255)                                                                                                                                    gCATGGTCAGCATGATCATGCGCGCAACCCAGAAGAAGATGATGTCGAAGCCAGTCACCAGCACGTCGGTGGAGTGGAACTTCTTGAGGAATTCGGTCTGCTCCGGCC‑AGCCCAGGGTGGAGAATGTCCACAGGCCCGAGCTGAACCAg          >  7:45760/1‑149 (MQ=255)                                                                                                                                     caaggTCATCAAGATCATGCGCGAAACACAGAAGAAGATGATGTCGAAGAAAGACACAAGCACGACGGTCGACAGGAACATCAAGATTAATTCGGTCTCATACCTCC‑AGCCCAGCGTGCAGAAAGTCCACAGGCCCGAGCTGAACCAg          >  5:232795/4‑148 (MQ=255)                                                                                                                                     cATGGTCAGCATGATCATGCGCGCAACCCAGAAGAAGATGATGTCGAAGCCAGTCACCAGCACGTCGGTGGAGTGGAACTTCTTGAGGAATTCGGTCTGCTCCGGCC‑AGCCCAGGGTGGAGAATGTCCACAGGCCCGAGCTGAACCAg          <  6:321891/148‑1 (MQ=255)                                                                                                                                        ggTCAGCATGATCATGCGCGCAACCCAGAAGAAGATGATGTCGAAGCCAGTCACCAGCACGTCGGTGGAGTGGAACTTCTTGAGGAATTCGGTCTGCTCCGGCC‑AGCCCAGGGTGGAGAATGTCCACAGGCCCGAGCTGAACCAGgtgt      >  8:183061/1‑149 (MQ=255)                                                                                                                                          tCAGCATGATCATGCGCGCAACCCAGAAGAAGATGATGTCGAAGCCAGTCACCAGCACGTCGGTGGAGTGGAACTTCTTGAGGAATTCGGTCTGCTCCGGCC‑AGCCCAGGGTGGAGAATGTCCACAGGCCCGAGCTGAACCAGGTGTc     >  7:110916/1‑148 (MQ=255)                                                                                                                                           cAGCATGATCATGCGCGCAACCCAGAAGAAGATGATGTCGAAGCCAGTCACCAGCACGTCGGTGGAGTGGAACTTCTTGAGGAATTCGGTCTGCTCCGGCC‑AGCCCAGGGTGGAGAATGTCCACAGGCCCGAGCTGAACCAGGTGTc     >  7:115180/1‑147 (MQ=255)                                                                                                                                            aGCATGATCATGCGCGCAACCCAGAAGAAGATGATGTCGAAGCCAGTCACCAGCACGTCGGTGGAGTGGAACTTCTTGAGGAATTCGGTCTGCTCCGGCC‑AGCCCAGGGTGGAGAATGTCCACAGGCCCGAGCTGAACCAGGTGTCGAg  <  8:110916/149‑1 (MQ=255)                                                                                                                                            aGCATGATCATGCGCGCAACACAGAAGAAGATGATGTCGAAGCCAGTCACCAGCACGTCGGTGGAGTGGAACTTCTTGAGGAATTCGGTCTGCTCCGGCC‑AGCCCAGGGTGGAGAATGTCCACAGGCCCGAGCTGAACCAGGTGTCGa   <  8:45760/148‑1 (MQ=255)                                                                                                                                                 gATCATGCGCGCAACCCAGAAGAAGATTATGTCGAAGCCAGTCACCAGCACGTCGGTGGAGTGGAACTTCTTGAGGAATTCGGTCTGCTCCGGCC‑AGCCCAGGGTGGAGAATGTCCACAGGCCCGAGCTGAAc             <  6:194489/133‑1 (MQ=255)                                                                                                                                                 gATCATGCGCGCAACCCAGAAGAAGATGATGTCGAAGCCAGTCACCAGCACGTCGGTGGAGTGGAACTTCTTGAGGAATTCGGTCTGCTCCGGCC‑AGCCCAGGGTGGAGAATGTCCACAGGCCCGAGCTGAAc             >  5:194489/1‑133 (MQ=255)                                                                                                                                                   |                                                                                                                                               GATGTCCAGCGGGTCCAGCACGTTGCCCTTGGACTTGGACATCTTCTGGCCCTGGCCGTCACGTACCAGGCCGTGCACGTACACGGTCTTGAACGGTACCTGCGGGGTGCCGTCTTCGTTCTTGATCAGGTGCATGGTCAGCATGATCATGCGCGCAACCCAGAAGAAGATGATGTCGAAGCCAGTCACCAGCACGTCGGTGGAGTGGAACTTCTTGAGGAATTCGGTCTGCTCCGGCC‑AGCCCAGGGTGGAGAATGTCCACAGGCCCGAGCTGAACCAGGTGTCGAG  >  NC_002947/1116137‑1116424

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑