Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,366,219 G→C 7.6% R161G (CGT→GGT)  PP_1191 ← S4 domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,366,2190GC 7.6% 204.4 / 6.3 66R161G (CGT→GGT) PP_1191S4 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base G (32/29);  new base C (2/3);  total (34/32)
Fisher's exact test for biased strand distribution p-value = 6.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.15e-01

GCATGCCTAAAAGCTCCAGGCGCAGGTAGGCGCATAGCTCGGAAATCTGTCCTGGTTGTGGGTTTTTCAACACCATGCGCAGGTGTGTCTCGTTCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGCGACGCAGGTCGGCCTCGATCTTGCGGGATACCCGCCAGTCCTGGGTAAACACCTGCAGGCCGCTGGCGCCACGCTC  >  NC_002947/1366072‑1366363
                                                                                                                                                   |                                                                                                                                                
gCATGCCTAAAAGCTCCAGGCGCAGGTAGGCGCATAGCTCGGAAATCTGTCCTGGTTGTGGGTTTTTCAACACCATGCGCAGGTGTGTCTCGTTCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCAcgc                                                                                                                                                 >  2:346272/1‑149 (MQ=255)
  aTGCCTAAAAGCTCCAGGCGCAGGTAGGCGCATAGCTCGGAAATCTGTCCTGGTTGTGGGTTTTTCAACACCATGCGCAGGTGTGTCTCGTTCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCACgcgc                                                                                                                                               >  8:324401/1‑149 (MQ=255)
  aTGCCTAAAAGCTCCAGGCGCAGGTAGGCGCATAGCTCGGAAATCTGTCCTGGTTGTGGGTTTTTAAACCCCATGCGCAGGTGTGTAACGTTCTGCCAGCTTGCCTTGGGCTTGGGAAACTCGGGGTCATTGAGCATGGCCCCACgcgc                                                                                                                                               >  1:301715/1‑149 (MQ=255)
     ccTAAAAGCTCCAGGCGCAGGTAGGCGCATAGCTCGGAAATCTGTCCTGGTTGTGGGTTTTTCAACACCATGCGCAGGTGTGTCTCGTTCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAg                                                                                                                                            <  1:101199/149‑1 (MQ=255)
      cTAAAAGCTCCAGGCGCAGGTAGGCGCATAGCTCGGAAATCTGTCCTGGTTGTGGGTTTTTCAACACCATGCGCAGGTGTGTCTCGTTCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCTCATGGCCCCACGCGCCAgg                                                                                                                                           >  8:323806/1‑149 (MQ=255)
         aaaGCTCCAGGCGCAGGTAGGCGCATAGCTCGGAAATCTGTCCTGGTTGTGGGTTTTTAAACACCATGCGCAGGTGTGTCTCGTTCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGt                                                                                                                                        >  5:53744/1‑149 (MQ=255)
         aaaGCTCCAGGCGCAGGTAGGCGCATAGCTCGGAAATCTGTCC‑GGTTGTGGGTTTTTCAACACCATGCGCAGGTGTGTCTCGTTCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGtt                                                                                                                                       >  4:350406/1‑149 (MQ=255)
            gatCCAGGCGAAGGTAGGCGCATAGCTCGGAAATCTGTCCTGGTTGTGGGTTTTTCAACACCATGCGCAGGTGTGTCTCGTTCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGAGCATGGCCCCACGCGCCATGCGTTc                                                                                                                                      >  2:338627/3‑148 (MQ=255)
              tCCAGGCGCAGGTAGGCGCATAGCTCGGAAATCTGTCCTGGTTGTGGGTTTTTCAACACCATGCGCAGGTGTGTCTCGTTCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAg                                                                                                                                   >  7:53592/1‑149 (MQ=255)
                cAGGCGCAGGTAGGCGCATAGCTCGGAAATCTGTCCTGGTTGTGGGTTTTTCAACACCATGCGCAGGTGTGTCTCGTTCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAgc                                                                                                                                  <  1:346272/148‑1 (MQ=255)
                  ggCGCAGGTAGGCGCATAGCTCGGAAATCTGTCCTGGTTGTGGGTTTTTCAACACCATGCGCAGGTGTGTCTCGTTCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGcc                                                                                                                               >  1:363955/1‑149 (MQ=255)
                   gcgcAGGTAGGCGCATAGCTCGGAAATCTGTCCTGGTTGTGGGTTTTTCAACACCATGCGCAGGTGTGTCTCGTTCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCt                                                                                                                              >  8:142326/1‑149 (MQ=255)
                       aGGTAGGCGCATAGCTCGGAAATCTGTCC‑GGTTGTGGGTTTTTCAACACCATGCGCAGGTGTGTCTCGTTCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTggggg                                                                                                                         <  3:350406/149‑1 (MQ=255)
                          tAGGCGCATAGCTCGGAAATCTGTCCTGGTTGTGGGTTTTTCAACACCATGCGCAGGTGTGTCTCGTTCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCACCCCCCAGGTGCTGGAACGCCTggcgcgt                                                                                                                       <  3:125099/149‑6 (MQ=255)
                             gcgcATAGCTCGGAAATCTGTCCTGGTTGTGGGTTTTTCAACACCATGCGCAGGTGTGTCTCGTTCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCACCCCCCAGGCGCTGGAACGCCTGGCGcgtggg                                                                                                                    <  1:17086/149‑2 (MQ=38)
                               gcATAGCTCGGAAATCTGTCCTGGTTGTGGGTTTTTCAACACCATGCGCAGGTGTGTCTCGTTCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGttt                                                                                                                  >  6:40274/1‑149 (MQ=255)
                                    gCTCGGAAATCTGTCCTGGTTGTGGGTTTTTCAACACCATGCGCAGGTGTGTCTCGTTCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTcgccg                                                                                                             <  8:53592/149‑1 (MQ=255)
                                     cTCGGAAATCTGTCCTGGTTGTGGGTTTTTCAACACCATGCGCAGGTGTGTCTCGTTCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTcgccgc                                                                                                            >  1:145991/1‑149 (MQ=255)
                                        ggAAATCTGTCCTGGTTGTGGGTTTTTCAACACCATGCGCAGGTGTGTCTCGTTCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCgcgc                                                                                                          <  2:363955/148‑1 (MQ=255)
                                           aaTCTGTCCTGGTTGTGGGTTTTTCAACACCAAGCGCACGTGTGTCTCGTTCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACtt                                                                                                      <  2:283426/149‑1 (MQ=255)
                                               tGTCCTGGTTGTGGGTTTTTCAACACCATGCGCAGGTGTGTCTCGTTCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAat                                                                                                  >  7:242675/1‑149 (MQ=255)
                                               tGTCCTGGTTGTGGGTTTTTCAACACCATGCGCAGGTGTGTCTCGTTCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAat                                                                                                  >  7:160205/1‑149 (MQ=255)
                                                 tCCTGGTTGTGGGTTTTTCAACACCATGCGCAGGTGTGTCTCGTTCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAatga                                                                                                <  2:145991/149‑1 (MQ=255)
                                                         gtgGGTTTTTCAACACCATGCGCAGGTGTGTCTCGTTCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTc                                                                                         <  7:142326/148‑1 (MQ=255)
                                                          tgGGTTTTTCAACACCATGCGCAGGTGTGTCTCGTTCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTcc                                                                                        <  8:242675/148‑1 (MQ=255)
                                                           gggTTTTTCAACACCATGCGCAGGTGTGTCTCGTTCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTg                                                                                      >  2:8440/1‑149 (MQ=255)
                                                           gggTTTTTCAACACCATGCGCAGGTGTGTCTCGTTCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTg                                                                                      >  7:33781/1‑149 (MQ=255)
                                                            ggTTTTTCAACACCATGCGCAGGTGTGTCTCGTTCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGc                                                                                     >  7:140492/1‑149 (MQ=255)
                                                            ggTTTTTCAACACCATGCGCAGGTGTGTCTCGTTCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGc                                                                                     >  6:91922/1‑149 (MQ=255)
                                                            ggTTTTTCAACACCATGCGCAGGTGTGTCTCGTTCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCACCACCCCCCAGGCGCTGGAACGCCTGGCGcgtggggccat                                                                                                               >  3:164259/1‑117 (MQ=25)
                                                            ggTTTTTCAACACCATGCGCAGGTGTGTCTCGTTCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCACCACCCCCCAGGCGCTGGAACGCCTGGCGcgtggggccat                                                                                                               <  4:164259/123‑7 (MQ=25)
                                                                    aaCACCATGCGCAGGTGTGTCTCGTTCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGc                                                                              <  3:209368/148‑1 (MQ=255)
                                                                    aaCACCATGCGCAGGTGTGTCTCGTTCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGc                                                                              <  4:304306/148‑1 (MQ=255)
                                                                     acacCATGCGCAGGTGTGTCTCGTTCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGCGa                                                                            <  5:91922/149‑1 (MQ=255)
                                                                         cATGCGCAGGTGTGTCTCGTTCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGCGACg                                                                          >  5:191665/1‑147 (MQ=255)
                                                                             cgcAGGTGTGTCTCGTTCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGCGACGCAGGTc                                                                    <  5:269337/149‑1 (MQ=255)
                                                                              gcAGGTGTGTCTCGTGCTGGCAGTTGGCCGTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGCGACGCAGGTCg                                                                   <  3:247409/149‑1 (MQ=255)
                                                                                aGGTGTGTCTCGTTCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCTCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGCGACGCAGGTCGGc                                                                 <  4:26171/149‑1 (MQ=255)
                                                                                aGGTGTGTCTCGTTCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGCGACGCAGGTCGGc                                                                 <  1:8440/149‑1 (MQ=255)
                                                                                      gtCTCGTTCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGCGACGCAGGTCGGCCTCGa                                                            >  7:249836/1‑148 (MQ=255)
                                                                                        ctcGTTCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGCGACGCAGGTCGGCCTCGATCt                                                         >  5:316198/1‑149 (MQ=255)
                                                                                            ttCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGCGACGCAGGTCGGCCTCGATCTTGCg                                                     >  7:102923/1‑149 (MQ=255)
                                                                                              cTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGCGACGCAGGTCGGCCTCGATCTTGCgg                                                    <  8:140492/148‑1 (MQ=255)
                                                                                              cTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGCGACGCAGGTCGGCCTCGATCTTGCgg                                                    >  5:380452/1‑148 (MQ=255)
                                                                                                 ccAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGCGACGCAGGTCGGCCTCGATCTTGCggg                                                   <  5:16059/146‑1 (MQ=255)
                                                                                                        gccttggccttgGGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGCGACGCAGGTCGGCCTCGATCTTGCGGGATACCCGc                                           <  7:133228/147‑1 (MQ=255)
                                                                                                           ttggccttggGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGCGACGCAGGTCGGCCTCGATCTTGCGGGATACCCGCCAGTc                                      >  2:49156/1‑149 (MQ=255)
                                                                                                             ggccttggGCAACTCGCGTTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTTGTTTCGCCGCTCACTTCAATGATGAACTCCTGCTCCAGGCGACGCAGGTCGGCCTCGATCTTGCGGGATACCCGCCAGTc                                      <  1:338627/147‑1 (MQ=255)
                                                                                                               ccttggGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGTTGAACTCCTGCTCCAGGCGACGCAGGTCGGCCTCGATCTTGCGGGATACCCGCCAGTCCTgg                                  <  7:324401/149‑1 (MQ=255)
                                                                                                               ccttggGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGCGACGCAGGTCGGCCTCGATCTTGCGGGATACCCGCCAGTCCTgg                                  <  8:249836/149‑1 (MQ=255)
                                                                                                               ccttggGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGCGACGCAGGTCGGCCTCGATCTTGCGGGATACCCGCCAGTCCTgg                                  <  5:40274/149‑1 (MQ=255)
                                                                                                               ccttggGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGCGACGCAGGTCGGCCTCGATCTTGCGGGATACCCGCCAGTCCTgg                                  <  3:85638/149‑1 (MQ=255)
                                                                                                                cttggGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGCGACGCAGGTCGGCCTCGATCTAGCGGGATAGCCGCCAGTCCTgcg                                 >  8:260781/1‑147 (MQ=255)
                                                                                                                    ggCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGCGACGCAGGTCGGCCTCGa                                                            <  8:16144/118‑1 (MQ=255)
                                                                                                                    ggCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGCGACGCAGGTCGGCCTCGa                                                            >  7:16144/1‑118 (MQ=255)
                                                                                                                     gCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGCGACGCAGGTCGGCCTCGATCTTGCGGGATACCCGCCAGTCCTGGGTaaa                             >  6:111362/1‑148 (MQ=255)
                                                                                                                        aCTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGCGACGCAGGTCGGCCTCGATCTTGCGGGATACCCGCCAGTCCTGGGTAAACAcc                         >  5:14745/1‑149 (MQ=255)
                                                                                                                          tCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGCGACGCAGGTCGGCCTCGATCTTGCGGGATACCCGCCAGTCCTGGGTAAACACCTg                       >  3:360448/1‑149 (MQ=255)
                                                                                                                          tCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGCGACGCAGGTCGGCCTCGATCTTGCGGGATACCCGCCAGTCCTGGGTAAACACCTg                       >  6:203886/1‑149 (MQ=255)
                                                                                                                            gcgGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGCGACGCAGGTCGGCCTCGATCTTGCGGGATACCCGCCAGTCCTGGGTAAACACCTg                       >  5:106427/1‑147 (MQ=255)
                                                                                                                            gcgGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGCGACGCAGGTCGGCCTCGATCTTGCGGGATACCCGCCAGTCCTGGGTAAACACCTg                       <  8:33781/147‑1 (MQ=255)
                                                                                                                            gcgGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGCGACGCAGGTCGGCCTCGATCTTGCGGGATACCCGCCAGTCCTGGGTAAACACCTGc                      <  1:49156/148‑1 (MQ=255)
                                                                                                                            gcgGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGCGACGCAGGTCGGCCTCGATCTTGCGGGATACCCGCCAGTCCTGGGTAAACACCTGCa                     <  1:186412/149‑1 (MQ=255)
                                                                                                                                      cgcATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGCGACGCAGGTCGGCCTCGATCTTGCGGGATACCCGCCAGTCCTGGGTAAACACCTGCAGGCCGCTGgc           >  3:357887/1‑149 (MQ=255)
                                                                                                                                          cgaaaccACCCCCCAGGCGCTGGAACGCCTGGCGCGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGCGACGCAGGTCGGCCTCGATCTTGCGGGATACCCGCCAGTCCTAGGTAAACACCTGCAGGCCGCTGGCGCCa       >  8:231454/6‑149 (MQ=37)
                                                                                                                                           ggCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGCGACGCAGGTCGGCCTCGATCTTGCGGGATACCCGCCAGTCCTGGGTAAACACCTGCAGGCCGCTGGCGCCAc      >  5:130105/1‑149 (MQ=255)
                                                                                                                                            gCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGCGACGCAGGTCGGCCTCGATCTTGCGGGATACCCGCCAGTCCTGGGTAAACACCTGCAGGCCGCTGGCGCCAc      <  8:344556/148‑1 (MQ=255)
                                                                                                                                              cccACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGCGACGCAGGTCGGCCTCGATCTTGCGGGATACCCGCCAGTCCTGGGTAAACACCTGCAGGCCGCTGGCGCCACGCt   >  6:187383/1‑149 (MQ=255)
                                                                                                                                               ccACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGCGACGCAGGTCGGCCTCGATCTTGCGGGATACCCGCCAGTCCTGGGTAAACACCTGCAGGCCGCTGGCGCCACGCTc  >  8:250501/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
GCATGCCTAAAAGCTCCAGGCGCAGGTAGGCGCATAGCTCGGAAATCTGTCCTGGTTGTGGGTTTTTCAACACCATGCGCAGGTGTGTCTCGTTCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGCGACGCAGGTCGGCCTCGATCTTGCGGGATACCCGCCAGTCCTGGGTAAACACCTGCAGGCCGCTGGCGCCACGCTC  >  NC_002947/1366072‑1366363

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑