Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,366,231 C→G 7.7% E157Q (GAA→CAA) ‡ PP_1191 ← S4 domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,366,2310CG 7.7% 202.8 / 7.1 65E157Q (GAA→CAA) ‡PP_1191S4 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base C (29/31);  new base G (2/3);  total (31/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.74e-01

GCTCCAGGCGCAGGTAGGCGCATAGCTCGGAAATCTGTCCTGGTTGTGGGTTTTTCAACACCATGCGCAGGTGTGTCTCGTTCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGCGACGCAGGTCGGCCTCGATCTTGCGGGATACCCGCCAGTCCTGGGTAAACACCTGCAGGCCGCTGGCGCCACGCTCCAGCGGTGCCAC  >  NC_002947/1366084‑1366375
                                                                                                                                                   |                                                                                                                                                
gatCCAGGCGAAGGTAGGCGCATAGCTCGGAAATCTGTCCTGGTTGTGGGTTTTTCAACACCATGCGCAGGTGTGTCTCGTTCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGAGCATGGCCCCACGCGCCATGCGTTc                                                                                                                                                  >  2:338627/3‑148 (MQ=255)
  tCCAGGCGCAGGTAGGCGCATAGCTCGGAAATCTGTCCTGGTTGTGGGTTTTTCAACACCATGCGCAGGTGTGTCTCGTTCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAg                                                                                                                                               >  7:53592/1‑149 (MQ=255)
    cAGGCGCAGGTAGGCGCATAGCTCGGAAATCTGTCCTGGTTGTGGGTTTTTCAACACCATGCGCAGGTGTGTCTCGTTCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAgc                                                                                                                                              <  1:346272/148‑1 (MQ=255)
      ggCGCAGGTAGGCGCATAGCTCGGAAATCTGTCCTGGTTGTGGGTTTTTCAACACCATGCGCAGGTGTGTCTCGTTCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGcc                                                                                                                                           >  1:363955/1‑149 (MQ=255)
       gcgcAGGTAGGCGCATAGCTCGGAAATCTGTCCTGGTTGTGGGTTTTTCAACACCATGCGCAGGTGTGTCTCGTTCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCt                                                                                                                                          >  8:142326/1‑149 (MQ=255)
           aGGTAGGCGCATAGCTCGGAAATCTGTCC‑GGTTGTGGGTTTTTCAACACCATGCGCAGGTGTGTCTCGTTCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTggggg                                                                                                                                     <  3:350406/149‑1 (MQ=255)
              tAGGCGCATAGCTCGGAAATCTGTCCTGGTTGTGGGTTTTTCAACACCATGCGCAGGTGTGTCTCGTTCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCACCCCCCAGGTGCTGGAACGCCTggcgcgt                                                                                                                                   <  3:125099/149‑6 (MQ=255)
                 gcgcATAGCTCGGAAATCTGTCCTGGTTGTGGGTTTTTCAACACCATGCGCAGGTGTGTCTCGTTCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCACCCCCCAGGCGCTGGAACGCCTGGCGcgtggg                                                                                                                                <  1:17086/149‑2 (MQ=38)
                   gcATAGCTCGGAAATCTGTCCTGGTTGTGGGTTTTTCAACACCATGCGCAGGTGTGTCTCGTTCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGttt                                                                                                                              >  6:40274/1‑149 (MQ=255)
                        gCTCGGAAATCTGTCCTGGTTGTGGGTTTTTCAACACCATGCGCAGGTGTGTCTCGTTCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTcgccg                                                                                                                         <  8:53592/149‑1 (MQ=255)
                         cTCGGAAATCTGTCCTGGTTGTGGGTTTTTCAACACCATGCGCAGGTGTGTCTCGTTCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTcgccgc                                                                                                                        >  1:145991/1‑149 (MQ=255)
                            ggAAATCTGTCCTGGTTGTGGGTTTTTCAACACCATGCGCAGGTGTGTCTCGTTCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCgcgc                                                                                                                      <  2:363955/148‑1 (MQ=255)
                               aaTCTGTCCTGGTTGTGGGTTTTTCAACACCAAGCGCACGTGTGTCTCGTTCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACtt                                                                                                                  <  2:283426/149‑1 (MQ=255)
                                   tGTCCTGGTTGTGGGTTTTTCAACACCATGCGCAGGTGTGTCTCGTTCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAat                                                                                                              >  7:242675/1‑149 (MQ=255)
                                   tGTCCTGGTTGTGGGTTTTTCAACACCATGCGCAGGTGTGTCTCGTTCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAat                                                                                                              >  7:160205/1‑149 (MQ=255)
                                     tCCTGGTTGTGGGTTTTTCAACACCATGCGCAGGTGTGTCTCGTTCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAatga                                                                                                            <  2:145991/149‑1 (MQ=255)
                                             gtgGGTTTTTCAACACCATGCGCAGGTGTGTCTCGTTCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTc                                                                                                     <  7:142326/148‑1 (MQ=255)
                                              tgGGTTTTTCAACACCATGCGCAGGTGTGTCTCGTTCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTcc                                                                                                    <  8:242675/148‑1 (MQ=255)
                                               gggTTTTTCAACACCATGCGCAGGTGTGTCTCGTTCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTg                                                                                                  >  2:8440/1‑149 (MQ=255)
                                               gggTTTTTCAACACCATGCGCAGGTGTGTCTCGTTCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTg                                                                                                  >  7:33781/1‑149 (MQ=255)
                                                ggTTTTTCAACACCATGCGCAGGTGTGTCTCGTTCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGc                                                                                                 >  6:91922/1‑149 (MQ=255)
                                                ggTTTTTCAACACCATGCGCAGGTGTGTCTCGTTCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGc                                                                                                 >  7:140492/1‑149 (MQ=255)
                                                ggTTTTTCAACACCATGCGCAGGTGTGTCTCGTTCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCACCACCCCCCAGGCGCTGGAACGCCTGGCGcgtggggccat                                                                                                                           >  3:164259/1‑117 (MQ=25)
                                                ggTTTTTCAACACCATGCGCAGGTGTGTCTCGTTCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCACCACCCCCCAGGCGCTGGAACGCCTGGCGcgtggggccat                                                                                                                           <  4:164259/123‑7 (MQ=25)
                                                        aaCACCATGCGCAGGTGTGTCTCGTTCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGc                                                                                          <  4:304306/148‑1 (MQ=255)
                                                        aaCACCATGCGCAGGTGTGTCTCGTTCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGc                                                                                          <  3:209368/148‑1 (MQ=255)
                                                         acacCATGCGCAGGTGTGTCTCGTTCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGCGa                                                                                        <  5:91922/149‑1 (MQ=255)
                                                             cATGCGCAGGTGTGTCTCGTTCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGCGACg                                                                                      >  5:191665/1‑147 (MQ=255)
                                                                 cgcAGGTGTGTCTCGTTCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGCGACGCAGGTc                                                                                <  5:269337/149‑1 (MQ=255)
                                                                  gcAGGTGTGTCTCGTGCTGGCAGTTGGCCGTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGCGACGCAGGTCg                                                                               <  3:247409/149‑1 (MQ=255)
                                                                    aGGTGTGTCTCGTTCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCTCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGCGACGCAGGTCGGc                                                                             <  4:26171/149‑1 (MQ=255)
                                                                    aGGTGTGTCTCGTTCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGCGACGCAGGTCGGc                                                                             <  1:8440/149‑1 (MQ=255)
                                                                          gtCTCGTTCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGCGACGCAGGTCGGCCTCGa                                                                        >  7:249836/1‑148 (MQ=255)
                                                                            ctcGTTCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGCGACGCAGGTCGGCCTCGATCt                                                                     >  5:316198/1‑149 (MQ=255)
                                                                                ttCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGCGACGCAGGTCGGCCTCGATCTTGCg                                                                 >  7:102923/1‑149 (MQ=255)
                                                                                  cTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGCGACGCAGGTCGGCCTCGATCTTGCgg                                                                >  5:380452/1‑148 (MQ=255)
                                                                                  cTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGCGACGCAGGTCGGCCTCGATCTTGCgg                                                                <  8:140492/148‑1 (MQ=255)
                                                                                     ccAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGCGACGCAGGTCGGCCTCGATCTTGCggg                                                               <  5:16059/146‑1 (MQ=255)
                                                                                            gccttggccttgGGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGCGACGCAGGTCGGCCTCGATCTTGCGGGATACCCGc                                                       <  7:133228/147‑1 (MQ=255)
                                                                                               ttggccttggGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGCGACGCAGGTCGGCCTCGATCTTGCGGGATACCCGCCAGTc                                                  >  2:49156/1‑149 (MQ=255)
                                                                                                 ggccttggGCAACTCGCGTTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTTGTTTCGCCGCTCACTTCAATGATGAACTCCTGCTCCAGGCGACGCAGGTCGGCCTCGATCTTGCGGGATACCCGCCAGTc                                                  <  1:338627/147‑1 (MQ=255)
                                                                                                   ccttggGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGTTGAACTCCTGCTCCAGGCGACGCAGGTCGGCCTCGATCTTGCGGGATACCCGCCAGTCCTgg                                              <  7:324401/149‑1 (MQ=255)
                                                                                                   ccttggGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGCGACGCAGGTCGGCCTCGATCTTGCGGGATACCCGCCAGTCCTgg                                              <  5:40274/149‑1 (MQ=255)
                                                                                                   ccttggGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGCGACGCAGGTCGGCCTCGATCTTGCGGGATACCCGCCAGTCCTgg                                              <  8:249836/149‑1 (MQ=255)
                                                                                                   ccttggGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGCGACGCAGGTCGGCCTCGATCTTGCGGGATACCCGCCAGTCCTgg                                              <  3:85638/149‑1 (MQ=255)
                                                                                                    cttggGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGCGACGCAGGTCGGCCTCGATCTAGCGGGATAGCCGCCAGTCCTgcg                                             >  8:260781/1‑147 (MQ=255)
                                                                                                        ggCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGCGACGCAGGTCGGCCTCGa                                                                        >  7:16144/1‑118 (MQ=255)
                                                                                                        ggCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGCGACGCAGGTCGGCCTCGa                                                                        <  8:16144/118‑1 (MQ=255)
                                                                                                         gCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGCGACGCAGGTCGGCCTCGATCTTGCGGGATACCCGCCAGTCCTGGGTaaa                                         >  6:111362/1‑148 (MQ=255)
                                                                                                            aCTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGCGACGCAGGTCGGCCTCGATCTTGCGGGATACCCGCCAGTCCTGGGTAAACAcc                                     >  5:14745/1‑149 (MQ=255)
                                                                                                              tCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGCGACGCAGGTCGGCCTCGATCTTGCGGGATACCCGCCAGTCCTGGGTAAACACCTg                                   >  3:360448/1‑149 (MQ=255)
                                                                                                              tCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGCGACGCAGGTCGGCCTCGATCTTGCGGGATACCCGCCAGTCCTGGGTAAACACCTg                                   >  6:203886/1‑149 (MQ=255)
                                                                                                                gcgGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGCGACGCAGGTCGGCCTCGATCTTGCGGGATACCCGCCAGTCCTGGGTAAACACCTg                                   <  8:33781/147‑1 (MQ=255)
                                                                                                                gcgGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGCGACGCAGGTCGGCCTCGATCTTGCGGGATACCCGCCAGTCCTGGGTAAACACCTg                                   >  5:106427/1‑147 (MQ=255)
                                                                                                                gcgGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGCGACGCAGGTCGGCCTCGATCTTGCGGGATACCCGCCAGTCCTGGGTAAACACCTGc                                  <  1:49156/148‑1 (MQ=255)
                                                                                                                gcgGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGCGACGCAGGTCGGCCTCGATCTTGCGGGATACCCGCCAGTCCTGGGTAAACACCTGCa                                 <  1:186412/149‑1 (MQ=255)
                                                                                                                          cgcATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGCGACGCAGGTCGGCCTCGATCTTGCGGGATACCCGCCAGTCCTGGGTAAACACCTGCAGGCCGCTGgc                       >  3:357887/1‑149 (MQ=255)
                                                                                                                              cgaaaccACCCCCCAGGCGCTGGAACGCCTGGCGCGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGCGACGCAGGTCGGCCTCGATCTTGCGGGATACCCGCCAGTCCTAGGTAAACACCTGCAGGCCGCTGGCGCCa                   >  8:231454/6‑149 (MQ=37)
                                                                                                                               ggCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGCGACGCAGGTCGGCCTCGATCTTGCGGGATACCCGCCAGTCCTGGGTAAACACCTGCAGGCCGCTGGCGCCAc                  >  5:130105/1‑149 (MQ=255)
                                                                                                                                gCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGCGACGCAGGTCGGCCTCGATCTTGCGGGATACCCGCCAGTCCTGGGTAAACACCTGCAGGCCGCTGGCGCCAc                  <  8:344556/148‑1 (MQ=255)
                                                                                                                                  cccACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGCGACGCAGGTCGGCCTCGATCTTGCGGGATACCCGCCAGTCCTGGGTAAACACCTGCAGGCCGCTGGCGCCACGCt               >  6:187383/1‑149 (MQ=255)
                                                                                                                                   ccACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGCGACGCAGGTCGGCCTCGATCTTGCGGGATACCCGCCAGTCCTGGGTAAACACCTGCAGGCCGCTGGCGCCACGCTc              >  8:250501/1‑149 (MQ=255)
                                                                                                                                          gctggCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGACCTCCTGCTCCAGGCGACGCAGGTCGGCCTCGATCTTGCGGGATACCCGCCAGTCCTGGGTAAACACCTGCAGGCCGCTGGCGCCACGCTCCAGCGGt       <  6:53744/146‑1 (MQ=255)
                                                                                                                                          ccAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGCGACGCAGGTCGGCCTCGATCTTGCGGGATACCCGCCAGTCCTGGGTAAACACCTGCAGGCCGCTGGCGCCACGCTCCAGCGGt       <  7:260781/149‑1 (MQ=255)
                                                                                                                                           cAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGCGACGCAGGTCGGCCTCGATCTTGCGGGATACCCGCCAGTCCTGGGTAAACACCTGCAGGCCGCTGGCGCCACGCTCCAGCGGTg      >  3:104044/1‑149 (MQ=255)
                                                                                                                                               cGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGCGACGCAGGTCGGCCTCGATCTTGCGGGATACCCGCCAGTCCTGGGTAAACACCTGCAGGCCGCTGGCGCCACGCTCCAGCGGTGCCAc  <  6:130105/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
GCTCCAGGCGCAGGTAGGCGCATAGCTCGGAAATCTGTCCTGGTTGTGGGTTTTTCAACACCATGCGCAGGTGTGTCTCGTTCTGCCAGCTGGCCTTGGCCTTGGGCAACTCGCGGTCATTGCGCATGGCCCCACGCGCCAGGCGTTCCAGCGCCTGGGGGGTGGTTTCGCCGCGCACTTCAATGATGAACTCCTGCTCCAGGCGACGCAGGTCGGCCTCGATCTTGCGGGATACCCGCCAGTCCTGGGTAAACACCTGCAGGCCGCTGGCGCCACGCTCCAGCGGTGCCAC  >  NC_002947/1366084‑1366375

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑