Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,384,896 C→T 5.6% M45I (ATG→ATA)  proS ← proline‑‑tRNA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,384,8960CT 5.6% 214.1 / 2.3 71M45I (ATG→ATA) proSproline‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base C (34/33);  new base T (2/2);  total (36/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.85e-01

GCAGCTCAGGGCCGTATTGCTCCCAGCGACCGGATTCCTGCCACAGCTCGGCGGGCTGGATGCTCGGCATCAGCACTTCCAGGGCGCCGGCGGCGTTCATTTCCTCACGCACCACGGCTTCGACCTTGCGCATTACCCGCAAGCCCATCGGCAGCCAGGTGTACAGGCCGGAGGCCAGTTTGCGGATCATGCCGGCACGCAGCATGAGCTGATGGCTGATGACCACTGCGTCGGCAGGGGTTTCTTTCTGGGTGGCGAGCAAATATTGACTGGTGCGCATGGTTAGCCGTGTC  >  NC_002947/1384751‑1385043
                                                                                                                                                 |                                                                                                                                                   
gcagcTCAGGGCCGTATTGCTCCCAGCGACCGGATTCCTGCCACAGCTCGGCGGGCTGGATGCTCGGCATCAGCACTTCCAGGGCGCCGGCGGCGTTCATTTCCTCACGCACCACGGCTTCGACCTTGCGCATTACTCGCAAGCCCATc                                                                                                                                                  >  5:38155/1‑149 (MQ=255)
        gggCCGTATTGCTCCCAGCGACCGGATTCCTGCCACAGCTCGGCGGGCTGGATGCTCGGCATCAGCACTTCCAGGGCGCCGGCGGCGTTCATTTCCTCACGCACCACGGCTTCGACCTTGCGCATTACTCGCAAGCCCATCGGCAGCCa                                                                                                                                          <  6:38155/149‑1 (MQ=255)
         ggCCGTATTGCTCCCAGCGACCGGATTCCTGCCACAGCTCGGCGGGCTGGATGCTCGGCATCAGCACTTCCAGGGCGCCGGCGGCGTTCATTTCCTCACGCACCACGGCTTCGACCTTGCGCATTACCCGCAAGCCCATCGGCAGCCAg                                                                                                                                         <  2:157147/149‑1 (MQ=255)
              tATTGCTCCCAGCGACCGGATTCCTGCCACAGCTCGGCGGGCTGGATGCTCGGCATCAGCACTTCCAGGGCGCCGGCGGCGTTCATTTCCTCACGCACCACGGCTTCGACCTTGCGCATTACCCGCAAGCCCATCGGCAGCCAGgtgt                                                                                                                                     >  6:297912/1‑148 (MQ=255)
                   cTCCCAGCGACCGGATTCCTGCCACAGCTCGGCGGGCTGGATGCTCGGCATCAGCACTTCCAGGGCGCCGGCGGCGTTCATTTCCTCACGCACCACGGCTTCGACCTTGCGCATTACCCGCAAGCCCATCGGCAGCCAGGTGTACAgg                                                                                                                                <  1:4947/148‑1 (MQ=255)
                      ccAGCGACCGGATTCCTGCCACAGCTCGGCGGGCTGGATGCTCGGCATCAGCACTTCCAGGGCGCCGGCGGCGTTCATTTCCTCACGCACCACGGCTTCGACCTTGCGCATTACCCGCAAGCCCATCGGCAGCCAGGTGTACAGGCCg                                                                                                                             <  8:269150/148‑1 (MQ=255)
                       cAGCGACCGGATTCCTGCCACAGCTCGGCGGGCTGGATGCTCGGCATCAGCACTTCCAGGGCGCCGGCGGCGTTCATTTCCTCACGCACCACGGCTTCGACCTTGCGCATTACCCGCAAGCCCATCGGCAGCCAGGTGTACAGGCCGGa                                                                                                                           <  6:219984/149‑1 (MQ=255)
                        aGCGACCGGATTCCTGCCACAGCTCGGCGGGCTGGATGCTCGGCATCAGCACTTCCAGGGCGCCGGCGGCGTTCATTTCCTCACGCACCACGGCTTCGACCTTGCGCATTACCCGCAAGCCCATCGGCAGCCAGGTGTACAGGCCGGAg                                                                                                                          <  4:358301/149‑1 (MQ=255)
                          cGACCGGATTCCTGCCACAGCTCGGCGGGCTGGATGCTCGGCATCAGCACTTCCAGGGCGCCGGCGGCGTTCATTTCCTCACGCACCACGGCTTCGACCTTGCGCATTACCCGCAAGCCCATCGGCAGCCAGGTGTACAGGCCGGAGGc                                                                                                                        <  1:114979/149‑1 (MQ=255)
                            aCCGGATTCCTGCCACAGCTCGGCGGGCTGGATGCTCGGCATCAGCACTTCCAGGGCGCCGGCGGCGTTCATTTCCTCACGCACCACGGCTTCGACCTTGCGCATTACCCGCAAGCCCATCGGCAGCCAGGTGTACAGGCCGGAGGCCa                                                                                                                      <  2:320838/149‑1 (MQ=255)
                              cGGATTCCTGCCACAGCTCGGCGGGCTGGATGCTCGGCATCAGCACTTCCAGGGCGCCGGCGGCGTTCATTTCCTCACGCACCACGGCTTCGACCTTGCGCATTACCCGCAAGCCCATCGGCAGCCAGGTGTACAGGCCGGAGGCCAGt                                                                                                                    >  3:135471/1‑149 (MQ=255)
                                gATTCCTGCCACAGCTCGGCGGGCTGGATGCTCGGCATCAGCACTTCCAGGGCGCCGGCGGCGTTCATTTCCTCACGCACCACGGCTTCGACCTTGCGCATTACCCGCAAGCCCATCGGCAGCCAGGTGTACAGGCCGGAGGCCAGttt                                                                                                                  >  5:154559/1‑149 (MQ=255)
                                gATTCCTGCCACAGCTCGGCGGGCTGGATGCTCGGCATCAGCACTTCCAGGGCGCCGGCGGCGTTCATTTCCTCACGCACCACGGCTTCGACCTTGCGCATTACCCGCAAGCCCATCGGCAGCCAGGTGTACAGGCCGGAGGCCAGttt                                                                                                                  >  1:185279/1‑149 (MQ=255)
                                     cTGCCACAGCTCGGCGGGCTGGATGCTCGGCATCAGCACTTCCAGGGCGCCGGCGGCGTTCATTTCCTCACGCACCACGGCTTCGACCTTGCGCATTACCCGCAAGCCCATCGGCAGCCAg                                                                                                                                         >  6:334622/1‑121 (MQ=255)
                                     cTGCCACAGCTCGGCGGGCTGGATGCTCGGCATCAGCACTTCCAGGGCGCCGGCGGCGTTCATTTCCTCACGCACCACGGCTTCGACCTTGCGCATTACCCGCAAGCCCATCGGCAGCCAg                                                                                                                                         <  5:334622/121‑1 (MQ=255)
                                     cTGCCACAGCTCGGCGGGCTGGATGCTCGGCATCAGCACTTCCAGGGCGCCGGCGGCGTTCATTTCCTCACGCACCACGGCTTCGACCTTGCGCATTACCCGCAAGCCCATCGGCAGCCAGGTGTACAGGCCGGAGGCCAGTTTGCGGa                                                                                                             >  2:138273/1‑149 (MQ=255)
                                            aGCTCGGCGGGCTGGATGCTCGGCATCAGCACTTCCAGGGCGCCGGCGGCGTTCATTTCCTCACGCACCACGGCTTCGACCTTGCGCATTACCCGCAAGCCCATCGGCAGCCAGGTGTACAGGCCGGAGGCCAGTTTGCGGATCATGcc                                                                                                      >  2:271414/1‑149 (MQ=255)
                                              cTCGGCGGGCTGGATGCTCGGCATCAGCACTTCCAGGGCGCCGGCGGCGTTCATTTCCTCACGCACCACGGCTTCGACCTTGCGCATTACCCGCAAGCCCATCGGCAGCCAGGTGTACAGGCCGGAGGCCAGTTTGCGGATCATGCCgg                                                                                                    >  3:169614/1‑149 (MQ=255)
                                               tCGGCGGGCTGGATGCTCGGCATCAGCACTTCCAGGGCGCCGGCGGCGTTCATTTCCTCACGCACCACGGCTTCGACCTTGCGCATTACCCGCAAGCCTATCGGCAGCCAGGTGTACCGGCCGGAGGCCAGTTTGCGGATCATGCCgg                                                                                                    >  7:12425/1‑148 (MQ=255)
                                               tCGGCGGGCTGGATGCTCGGCATCAGCACTTCCAGGGCGCCGGCGGCGTTCATTTCCTCACGCACCACGGCTTCGACCTTGCGCATTACCCGCAAGCCTATCGGCAGCCAGGTGTACAGGCCGGAGGCCAGTTTGCGGATCATGCCgg                                                                                                    >  7:17870/1‑148 (MQ=255)
                                                 ggcggGCTGGATTCTCGGCATCAGCACTTCCAGGGCGCCGGCGGAGTTCATTTCCTCACGCACCACGGCTTCGACCTTGCGCATTACCCGCAAGCCCATCGGCAGCCAGGTGTACAGGCCGGAGGCCAGTTTGCGGATCATGCCGGCAc                                                                                                 <  2:26167/149‑1 (MQ=255)
                                                    gggCTGGATGCTCGGCATCAGCACTTCCAGGGCGCCGGCGGCGTTCATTTCCTCACGCACCACGGCTTCGACCTTGCGCATTACCCGCAAGCCCATCGGCAGCCAGGTGTACAGGCCGGAGGCCAGTTTGCGGATCATGCCGGCACgca                                                                                              <  1:138273/149‑1 (MQ=255)
                                                          gATGCTCGGCATCAGCACTTCCAGGGCGCCGGCGGCGTTCATTTCCTCACGCACCACGGCTTCGACCTTGCGCATTACCCGCAAGCCCATCGGCAGCCAGGTGTACAGGCCGGAGGCCAGTTTGCGGATCATGCCGGCACGCAGCAt                                                                                          <  4:135471/147‑1 (MQ=255)
                                                           aTGCTCGGCATCAGCACTTCCAGGGCGCCGGCGGCGTTCATTTCCTCACGCACCACGGCTTCGACCTTGCGCATTACCCGCAAGCCCATCGGCAGCCAGGTGTACAGGCCGGAGGCCAGTTTGCGGATCATGCCGGCACGCAGCATGAg                                                                                       <  4:68719/149‑1 (MQ=255)
                                                            tGCTCGGCATCAGCACTTCCAGGGCGCCGGCGGCGTTCATTTCCTCACGCACCACGGCTTCGACCTTGCGCATTACCCGCAAGCCCATCGGCAGCCAGGTGTACAGGCCGGAGGGCAGTTTGCGGATCATGCCGGCACGCAGCATGAgc                                                                                      >  1:367129/1‑149 (MQ=255)
                                                               tCGGCATCAGCACTTCCAGGGCGCCGGCGGCGTTCATTTCCTCACGCACCACGGCTTCGACCTTGCGCATTACCCGCACGCCCATCGGCAGCCAGGTGTACAGGCCGGAGGCCAGTTTGCGGATCATGCCGGCACGCAGCATGAgctga                                                                                   >  1:358322/1‑149 (MQ=255)
                                                               tCGGCATCAGCACTTCCAGGGCGCCGGCGGCGTTCATTTCCTCACGCACCACGGCTTCGACCTTGCGCATTACCCGCAAGCCTATCGGCAGCCAGGTGTACAGGCCGGAGGCGAGTTTGCGGATCATGCCGGCACGCAGCATGAgctga                                                                                   <  8:12425/149‑1 (MQ=255)
                                                               tCGGCATCAGCACTTCCAGGGCGCCGGCGGCGTTCATTTCCTCACGCACCACGGCTTCGACCTTGCGCATTACCCGCAAGCCCATCGGCAGCCAGGTGTACAGGCCGGAGGCCAGTTTGCGGATCATGCCGGCACgc                                                                                               >  4:346062/1‑137 (MQ=255)
                                                               tCGGCATCAGCACTTCCAGGGCGCCGGCGGCGTTCATTTCCTCACGCACCACGGCTTCGACCTTGCGCATTACCCGCAAGCCCATCGGCAGCCAGGTGTACAGGCCGGAGGCCAGTTTGCGGATCATGCCGGCACgc                                                                                               <  3:346062/137‑1 (MQ=255)
                                                                 ggCATCAGCACTTCCAGGGCTCCGGCGGCGTTCATTTCCTCACGCACCACGGCTTCGACCTTGCGCATTACCCGCAAGCCCATCGGCAGCCAGGTGTACAGGCCGGAGGCCAGTTTGCGGATCATGCCGGCACGCAGCATGAgcagatg                                                                                 >  7:154433/1‑149 (MQ=255)
                                                                 ggCATCAGCACTTCCAGGGCGCCGGCGGCGTTCATTTCCTCACGCACCACGGCTTCGACCTTGCGCATTACCCGCAAGCCTATCGGCAGCCAGGTGTACAGGCCGGAGGCCAGTTTGCGGATCATGCCGGCACGCAGCATGAgctga                                                                                   <  8:17870/147‑1 (MQ=255)
                                                                  gCATCAGCACTTCCAGGGCGCCGGCGGCGTTCATTTCCTCACGCACCACGGCTGCGACCTTGCGCATTACCCGCAAGCCCATCGGCAGCCAGGTGTACAGGCCGGAGGCCAGTTTGCGGATCATGCCGGCACGCAGCATGAgctgatgg                                                                                <  5:297912/149‑1 (MQ=255)
                                                                    aTCAGCACTTCCAGGGCGCCGGCGGCGTTCATTTCCTCACGCACCACGGCTTCGACCTTGCGCATTACCCGCAAGCCCATCGGCAGCCAGGTGTACAGGCCGGAGGCCAGTTTGCGGATCATGCCGGCACGCAGCATGAgctgatggct                                                                              <  7:5700/149‑1 (MQ=255)
                                                                       aGCACTTCCAGGGCGCCGGCGGCGTTCATTTCCTCACGCACCACGGCTTCGACCTTGCGCATTACCCGCAAGCCCATCGGCAGCCAGGTGTACAGGCCGGAGGCCAGTTTGCGGATCATGCCGGCACGCAGCATGAgctgatggctgat                                                                           >  2:41500/1‑149 (MQ=255)
                                                                       aGCACTTCCAGGGCGCCGGCGGCGTTCATTTCCTCACGCACCACGGCTTCGACCTTGCGCATTACCCGCAAGCCCATCGGCAGCCAGGTGTACAGGCCGGAGGCCAGTTTGCGGATCATGCCGGCACGCAGCATGAgctgatggctgat                                                                           >  5:110286/1‑149 (MQ=255)
                                                                        gCACTTCCAGGGCGCCCGCGGCGTTCATTTCCTAACGCACCACGGCTTCGACCTTGCGCATTACCCGCAAGCCCAACGGCAGCCAGGTGTACAGGCCGCAGGCCAGTATGCGGATCATGCCGGCACGCAGCATGAgatgatggctgat                                                                           >  4:229617/1‑148 (MQ=255)
                                                                         cACTTCCAGGGCGCCGGCGGCGTTCATTTCCTCACGCACCACGGCTTCGACCTTGCGCATTACCCGCAAGCCCATCGGCAGCCAGGTGTACAGGCCGGAGGCCAGTTTGCGGATCATGCCGGCACGCAGCATGAgctgatgg                                                                                >  2:99551/1‑142 (MQ=255)
                                                                         cACTTCCAGGGCGCCGGCGGCGTTCATTTCCTCACGCACCACGGCTTCGACCTTGCGCATTACCCGCAAGCCCATCGGCAGCCAGGTGTACAGGCCGGAGGCCAGTTTGCGGATCATGCCGGCACGCAGCATGAgctgatgg                                                                                <  1:99551/142‑1 (MQ=255)
                                                                         cACTTCCAGGGCGCCGGCGGCGTTCATTTCCTCACGCACCACGGCTTCGACCTTGCGCATTACCCGCAAGCCCATCGGCAGCCAGGTGTACAGGCCGGAGGCCAGTTTGCGGATCATGCCGGCACGCAGCATGAGCTGATGGCtgatga                                                                         <  3:245548/149‑1 (MQ=255)
                                                                          aCTTCCAGGGCGCCGGCGGCGTTCATTTCCTCACGCACCACGGCTTCGACCTTGCGCATTACCCGCAAGCCCATCGGCAGCCAGGTGTACAGGCCGGAGGCCAGTTTGCGGATCATGCCGGCACGCAGCATGAgctgatggctga                                                                            >  6:214188/1‑145 (MQ=255)
                                                                          aCTTCCAGGGCGCCGGCGGCGTTCATTTCCTCACGCACCACGGCTTCGACCTTGCGCATTACCCGCAAGCCCATCGGCAGCCAGGTGTACAGGCCGGAGGCCAGTTTGCGGATCATGCCGGCACGCAGCATGAgctgatggctga                                                                            <  5:214188/145‑1 (MQ=255)
                                                                           cTTCCAGGGCGCCGGCGGCGTTCATTTCCTCACGCACCACGGCTTCGACCTTGCGCATTACCCGCAAGCCCATCGGCAGCCAGGTGTACAGGCCGGAGGCCAGTTTGCGGATCATGCCGGCACGCAGCATGAGCTGATGGCTGATGAc                                                                        >  7:84212/1‑148 (MQ=255)
                                                                            ttCCAGGGCGCCGGCGGCGTTCATTTCCTCACGCACCACGGCTTCGACCTTGCGCATTACCCGCAAGCCCATCGGCAGCCAGGTGTACAGGCCGGAGGCCAGTTTGCGGATCATGCCGGCACGCAGCATGAGCTGATGGCTGATGACCa                                                                      >  6:66994/1‑149 (MQ=255)
                                                                              ccAGGGCGCCGGCGGCGTTCATTTCCTCACGCACCACGGCTTCGACCTTGCGCATTACCCGCAAGCCCATCGGCAGCCAGGTGTACAGGCCGGAGGCCAGTTTGCGGATCATGCCGGCACGCAGCATGAGCTGATGGCTGATGACCACt                                                                    >  7:70795/1‑149 (MQ=255)
                                                                                         gcggcgTTCATTTCCTCACGCACCACGGCTTCGACCTTGCGCATTACCCGCAAGCCCATCGGCAGCCAGGTGTACAGGCCGGAGGCCAGTTTGCGGATCATGCTGGCACGCAGCATGAGCTGATGGCTGATGACCACTGCGTCGGCAgg                                                         <  1:79639/149‑1 (MQ=255)
                                                                                               ttCATTTCCTCACGCACCACGGCTTCGACCTTGCGCATTACCCGCAAGCCCATCGGCAGCCAGGTGTACAGGCCGGAGGCCAGTTTGCGGAt                                                                                                            >  4:359136/1‑92 (MQ=255)
                                                                                               ttCATTTCCTCACGCACCACGGCTTCGACCTTGCGCATTACCCGCAAGCCCATCGGCAGCCAGGTGTACAGGCCGGAGGCCAGTTTGCGGAt                                                                                                            <  3:359136/92‑1 (MQ=255)
                                                                                                     tCCTCACGCACCACGGCTTCGACCTTGCGCATTACCCGCAAGCCCATCGGCAGCCAGGTGTACAGGCCGGAGGCCAGTTTGCGGATCATGCCGGCACGCAGCATGAGCTGATGGCTGATGACCACTGCGTCGGCAGGGGTttctttct                                              <  3:191528/148‑1 (MQ=255)
                                                                                                     tCCTCACGCACCACGGCTTCGACCTTGCGCATTACCCGCAAGCCCATCGGCAGCCAGGTGTACAGGCCGGAGGCCAGTTTGCGGATCATGCCGGCACGCAGCATGAGCTGATGGCTGATGACCACTGCGTCGGCAGGGGTTTCTTTCTg                                             >  5:92464/1‑149 (MQ=255)
                                                                                                      ccTCACGCACCACGGCTTCGACCTTGCGCATTACCCGCAAGCCCATCGGCAGCCAGGTGTACAGGCCGGAGGCCAGTTTGCGGATCATGCCGGCACGCAGCATGAGCTGATGGCTGATGACCACTGCGTCGGCAGGGGTTTCTTTCTg                                             <  1:41500/148‑1 (MQ=255)
                                                                                                      ccTCACGCACCACGGCTTCGACCTTGCGCATTACCCGCAAGCCCATCGGCAGCCAGGTGTACAGGCCGGAGGCCAGTTTGCGGATCATGCCGGCACGCAGCATGAGCTGATGGCTGATGACCACTGCGTCGGCAGGGGTTTCTTTCTg                                             <  5:66994/148‑1 (MQ=255)
                                                                                                       cTCACGCACCACGGCTTCGACCTTGCGCATTACCCGCAAGCCCATCGGCAGCCAGGTGTACAGGCCGGAGGGCAGTTTGCGGATCATGCCGGCACGCAGCATGAGCTGATGGCTGATGACCACTGCGTCGGCAGGGGTTTCTTTCTgg                                            <  2:367129/148‑1 (MQ=255)
                                                                                                        tCACGCACCACGGCTTCGACCTTGCGCATTACCCGCAAGCCCATCGGCAGCCAGGTGTACAGGCCGGAGGCCAGTTTGCGGATCATGCCGGCACGCAGCATGAGCTGATGGCTGATGACCACTGCGTCGGCAGGGGTTTCTTTCTGGGt                                          >  2:265645/1‑149 (MQ=255)
                                                                                                        tCACGCACCACGGCTTCGACCTTGCGCATTACCCGCAAGCCCATCGGCAGCCAGGTGTACAGGCCGGAGGCCAGTTTGCGGATCATGCCGGCACGCAGCATGAGCTGATGGCTGATGACCACTGCGTCGGCAGGGGTTTCTTTCTGGGt                                          >  7:172861/1‑149 (MQ=255)
                                                                                                          aCGCACCACGGCTTCGACCTTGCGCATTACCCGCAAGCCCATCGGCAGCCAGGTGTACAGGCCGGAGGCCAGTTTGCGGATCATGCCGGCACGCAGCATGAGCTGATGGCTGATGACCACTGCGTCGGCAg                                                          <  8:164272/131‑1 (MQ=255)
                                                                                                          aCGCACCACGGCTTCGACCTTGCGCATTACCCGCAAGCCCATCGGCAGCCAGGTGTACAGGCCGGAGGCCAGTTTGCGGATCATGCCGGCACGCAGCATGAGCTGATGGCTGATGACCACTGCGTCGGCAg                                                          >  7:164272/1‑131 (MQ=255)
                                                                                                          aCGCACCACGGCTTCGACCTTGCGCATTACCCGCAAGCCCATCGGCAGCCAGGTGTACAGGCCGGAGGCCAGTTTGCGGATCATGCCGGCACGCAGCATGAGCTGATGGCTGATGACCACTGCGTCGGCAGGGGTTTCTTTCTGGGt                                          <  3:229617/147‑1 (MQ=255)
                                                                                                          aCGCACCACGGCTTCGACCTTGCGCATTACCCGCAAGCCCATCGGCAGCCAGGTGTACAGGCCGGAGGCCAGTTTGCGGATCATGCCGGCACGCAGCATGAGCTGATGGCTGATGACCACTGCGTCGGCAGGGGTTTCTTTCTGGGt                                          <  4:169614/147‑1 (MQ=255)
                                                                                                            gCACCACGGCTTCGACCTTGCGCATTACCCGCAAGCCCATCGGCAGCCAGGTGTACAGGCCGGAGGCCAGTTTGCGGATCATGCCGGCACGCAGCATGAGCTGATGGCTGATGACCACTGCGTCGGCAGGGGTTTCTTTCTGGGTGGCg                                      >  1:260279/1‑149 (MQ=255)
                                                                                                                cacGGCTTCGACCTTGCGCATTACCCGCAAGCCCATCGGCAGCCAGGTGTACAGGCCGGAGGCCAGTTTGTGGATCATGCCGGCACGCAGCATGAGCTGATGGCTGATGACCACTGCGTCGGCAGGGGTTTCTTTCTGGGTGGCGAGc                                   >  1:53379/1‑148 (MQ=255)
                                                                                                                cacGGCTTCGACCTTGCGCATTACCCGCAAGCCCATCGGCAGCCAGGTGTACAGGCCGGAGGCCAGTTTGCGGATCATGCCGGCACGCAGCATGAGCTGATGGCTGATGACCACTGCGTCGGCAGGGGTTTCTTTCTGGGTGGCGAGc                                   >  1:252900/1‑148 (MQ=255)
                                                                                                                      ttCGACCTTGCGCATTACCCGCAAGCCCATCGGCAGCCAGGTGTACAGGCCGGAGGCCAGTTTGCGGATCATGCCGGCACGCAGCATGAGCTGATGGCTGATGACCACTGCGTCGGCAGGGGTTTCTTTCTGGGTGGCGAGCAAATAtt                            >  1:42253/1‑149 (MQ=255)
                                                                                                                        cGACCTTGCGCATTACCCGCAAGCCCATCGGCAGCCAGGTGTACAGGCCGGAGGCCAGTTTGCGGATCATGCCGGCACGCAGCATGAGCTGATGGCTGATGACCACTGCGTCGGCAGGGGTTTCTTTCTGGGTGGCGAGCAAATAtt                            <  6:154559/147‑1 (MQ=255)
                                                                                                                        cGACCTTGCGCATTACCCGCAAGCCCATCGGCAGCCAGGTGTACAGGCCGGAGGCCAGTTTGCGGATCATGCCGGCACGCAGCATGAGCTGATGGCTGATGACCACTGCGTCGGCAGGGGTTTCTTTCTGGGTGGCGAGCAAATAtt                            <  2:185279/147‑1 (MQ=255)
                                                                                                                           ccTTGCGCATTACCCGCAAGCCCATCGGCAGCCAGGTGTACAGGCCGGAGGCCAGTTTGCGGATCATGCCGGCACGCAGCATGAGCTGATGGCTGATGACCACTGCGTCGGCAGGGGTTTCTTTCTGGGTGGCGAGCAAATATTGACTg                       >  2:60616/1‑149 (MQ=255)
                                                                                                                            cTTGCGCATTACCCGCAAGCCCATCGGCAGCCAGGTGTACAGGCCGGAGGCCAGTTTGCGGATCATGCCGGCACGCAGCATGAGCTGATGGCTGATGACCACTGCGTCGGCAGGGGTTTCTTTCTGGGTGGCGAGCAAATATTGACTgg                      <  6:110286/149‑1 (MQ=255)
                                                                                                                            cTTGCGCATTACCCGCAAGCCCATCGGCAGCCAGGTGTACAGGCCGGAGGCCAGTTTGCGGATCATGCCGGCACGCAGCATGAGCTGATGGCTGATGACCACTGCGTCGGCAGGGGTTTCTTTCTGGGTGGCGAGCAAATATTGACTgg                      >  7:40427/1‑149 (MQ=255)
                                                                                                                              tGCGCATTACCCGCAAGCCCATCGGCAGCCAGGTGTACAGGCCGGAGGCCAGTTTGCGGATCATGCCGGCACGCAGCATGAGCTGATGGCTGATGACCACTGCGTCGGCAGGGGTTTCTTTCTGGGTGGCGAGCAAATATTGACTGGTg                    >  4:65747/1‑149 (MQ=255)
                                                                                                                                    ttACCCGCAAGCCCATCGGCAGCCAGGTGTACAGGCCGGAGGCCAGTTTGCGGATCATGCCGGCACGCAGCATGAGCTGATGGCTGATGACCACTGCGTCGGCAGGGGTTTCTTTCTGGGTGGCGAGCAAATATTGACTGGTGCGCATg              <  1:271414/149‑1 (MQ=255)
                                                                                                                                        ccGCAAGCCCATCGGCAGCCAGGTGTACAGGCCGGAGGCCAGTTTGCGGATCATGCCGGCACGCAGCATGAGCTGATGGCTGATGACCACTGCGTCGGCAGGGGTTTCTTTGTGGGTGGCGAGCAAATATTGACTGGTGCGCATGGt            >  4:238323/1‑147 (MQ=255)
                                                                                                                                        ccGCAAGCCCATCGGCAGCCAGGTGTACAGGCCGGAGGCCAGTTTGCGGATCATGCCGGCACGCAGCATGAGCTGATGGCTGATGACCACTGCGTCGGCAGGGGTTTCTTTCTGGGTGGCGAGCAAATATTGTCTGGTGCGCATGGTTa          <  3:65747/149‑1 (MQ=255)
                                                                                                                                               cccATCGGCAGCCAGGTGTACAGGCCGGAGGCCAGTTTGCGGATCATGCCGGCACGCAGCATGAGCTGATGGCTGATGACCACTGCGTCGGCAGGGGTTTCTTTCTGGGTGGCGAGCAAATATTGACTGGTGCGCATGGTTAGCCgtgt   <  1:60616/149‑1 (MQ=255)
                                                                                                                                               cccATCGGCAGCCAGGTGTACAGGCCGGAGGCCAGTTTGCGGATCATGCCGGCACGCAGCATGAGCTGATGGCTGATGACCACTGCGTCGGCAGGGGTTTCTTTCTGGGTGGCGAGCAAATATTGACTGGTGCGCATGGTTAGCCgtgt   <  2:29629/149‑1 (MQ=255)
                                                                                                                                                ccATCGGCAGCCAGGTGTACAGGCCGGAGGCCAGTTTGCGGATCATGCCGGCACGCAGCATGCGCTGATGGCTGATGACCACTGCGTCGGCAGGGGTTTCTTTCTGGGTGGCGAGCAAATATTGACTGGTGCGCATGGTTAGCCGTGTc  >  1:248489/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
GCAGCTCAGGGCCGTATTGCTCCCAGCGACCGGATTCCTGCCACAGCTCGGCGGGCTGGATGCTCGGCATCAGCACTTCCAGGGCGCCGGCGGCGTTCATTTCCTCACGCACCACGGCTTCGACCTTGCGCATTACCCGCAAGCCCATCGGCAGCCAGGTGTACAGGCCGGAGGCCAGTTTGCGGATCATGCCGGCACGCAGCATGAGCTGATGGCTGATGACCACTGCGTCGGCAGGGGTTTCTTTCTGGGTGGCGAGCAAATATTGACTGGTGCGCATGGTTAGCCGTGTC  >  NC_002947/1384751‑1385043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑