Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,388,299 T→C 6.9% Q64R (CAG→CGG)  PP_1209 ← cold‑shock protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,388,2990TC 6.9% 250.6 / 6.2 86Q64R (CAG→CGG) PP_1209cold‑shock protein
Reads supporting (aligned to +/- strand):  ref base T (40/40);  new base C (3/3);  total (43/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

CGGGCAAAGCCCACGGCCAGATGCACGGCCACGGCCAGCGCCGCACACACCAGGGCTGCCAGGTTGCCCATTTCGGGGCGGGCCAGTAGCGTCAGGGTCT‑‑GCAGCACGACGGCGACTACCAGCAGGGCGCAGGCCAGGTGCTGCAGTTGTTGCCGCGCGCCGCGATGATACAGCGGCACGACCGGGGCCAGCACAAGGTTGAGCAGGCCGAGCA‑GGGCCAGGTAGACCGCGTCGGGTTGTTGCAGGAAGGGTGTCGCATCGGTTTTCAGGCTGGGTATGAGCGA  >  NC_002947/1388158‑1388441
                                                                                                                                               |                                                                                                                                               
cGGGCAAAGCCCACGGCCAGATGCACGGCCACGGCCAGCGCCGCACACACCAGGGCTGCCAGGTTGCCCATTTCGGGGCGGGCCAGTAGCGTCAGGGTCT‑‑GCAGCACGACGGCGACTACCAGCAGGGCGCAGGCCAGGTGCTGCAgt                                                                                                                                            <  7:130734/147‑1 (MQ=255)
cGGGCAAAGCCCACGGCCAGATGCACGGCCACGGCCAGCGCCGCACACACCAGGGCTGCCAGGTTGCCCATTTCGGGGCGGGCCAATAGCGTCAGGGTCT‑‑GCAGCACGACGGCGACTACCAGCAGGGCGCAGGCCAGGTGCCGCAgttg                                                                                                                                          >  4:195653/1‑149 (MQ=255)
  ggCAAAGCCCACGGCCAGATGCACGGCCACGGCCAGCGCCGCACACACCAGGGCTGCCAGGTTGCCCATTTCGGGGCGGGCCAGTAGCGTCAGGGTCT‑‑GCAGCACGACGGCGACTACCAGCAGGGCGCAGGCCAGGTGCTGCAgttgtt                                                                                                                                        <  5:86916/149‑1 (MQ=255)
   gCAAAGCCCACGGCCAGATGCACGGCCACGGCCAGCGCCGCACACACCAGGGCTGCCAGGTTGCCCATTTCGGGGCGGGCCAGTAGCGTCAGGGTCT‑‑GCAGCACGACGGCGACTACCAGCAGGGCGCAGGCCAGGTGCTGCAGttgttg                                                                                                                                       <  5:371122/149‑1 (MQ=255)
    cAAAGCCCACGGCCAGATGCACGGCCACGGCCAGCGCCGCACACACCAGGGCTGCCAGGTTGCCCATTTCGGGGCGGGCCAGTAGCGTCAGGGTCT‑‑GCAGCACGACGGCGACTACCAGCAGGGCGCAGGCCAGGTGCTGCAGTTGTTGc                                                                                                                                      >  7:300776/1‑149 (MQ=255)
         cccACGGCCAGATGCACGGCCACGGCCAGCGCCGCACACACCAGGGCTGCCAGGTTGCCCATTTCGGGGCGGGCCAGTAGCGTCAGGGTCT‑‑GCAGCACGACGGCGACTACCAGCAGGGCGCAGGCCAGGTGCTGCAGTTGTTGCcgcgc                                                                                                                                 >  2:282606/1‑149 (MQ=255)
              ggCCAGATGCACGGCCACGGCCAGCGCCGCACACACCAGGGCTGCCAGGTTGCCCATTTCGGGGCGGGCCAGTAGCGTCAGGGTCT‑‑GCAGCACGACGGCGACTACCAGCAGGGCGCAGGCCAGGTGCTGCAGTTGTTGCCGCGcgccgc                                                                                                                            <  5:112357/149‑1 (MQ=255)
                ccAGATGCACGGCCACGGCCAGCGCCGCACACAGCAGGGCTGCCAGGTTGCCCATTTCGGGGCGGGCCAGTAGCGTCAGGGTCT‑‑GCAGCACGACGGCGACTACCAGCAGGGCGCAGGCCAGGTGCTGCAGTTGTTGCCGCGCGCCGCga                                                                                                                          <  4:15273/149‑1 (MQ=255)
                ccAGATGCACGGCCACGGCCAGCGCCGCACACACCAGGGCTGCCAGGTTGCCCATTTCGGGGCGGGCCAGTAGCGTCAGGGTCT‑‑GCAGCACGACGGCGACTACCAGCAGGGCGCAGGCCAGGTGCTGCAGTTGTTGCCGCGCGCCGCga                                                                                                                          >  8:354541/1‑149 (MQ=255)
                ccAGATGCACGGCCACGGCCAGCGCCGCACACACCAGGGCTGCCAGGTTGCCCATTTCGGGGCGGGCCAGTAGCGTCAGGGTCT‑‑GCAGCACGACGGCGACTACCAGCAGGGCGCAGGCCAGGTGCTGCAGTTGTTGCCGCGCGCCGCga                                                                                                                          >  1:170147/1‑149 (MQ=255)
                 cAGATGCACGGCCACGGCCAGCGCCGCACACACCAGGGCTGCCAGGTTGCCCATTTCGGGGCGGGCCAGTAGCGTCAGGGTCT‑‑GCAGCACGACGGCGACTACCAGCAGGGCGCAGGCCAGGTGCTGCAGTTGTTGCCGCGCGCCGCgat                                                                                                                         <  5:212647/149‑1 (MQ=255)
                  aGATGCACGGCCACGGCCAGCGCCGCACACACCAGGGCTGCCAGGTTGCCCATTTCGGGGCGGGCCAGTAGCGTCAGGGTCT‑‑GCAGCACGACGGCGACTACCAGCAGGTCGCAGGCCAGGTGCTGCAGTTGTTGCCGCGCGCCGCgat                                                                                                                         >  6:368590/1‑148 (MQ=255)
                   gATGCACGGCCACGGCCAGCGCCGCACACACCAGGGCTGCCAGGTTGCCCATTTCGGGGCGGGCCAGTAGCGTCAGGGTCT‑‑GCAGCACGACGGCGACTACCAGCAGGGCGCAGGCCAGGTGCCGCAGTTGTTGCCGCGCGCCGCgatga                                                                                                                       >  3:259813/1‑149 (MQ=255)
                   gATGCACGGCCACGGCCAGCGCCGCACACACCAGGGCTGCCAGGTTGCCCATTTCGGGGCGGGCCAGTAGCGTCAGGGTCT‑‑GCAGCACGACGGCGACTACCAGCAGGGCGCAGGCCAGGTGCCGCAGTTGTTGCCGCGCGCCGCgatga                                                                                                                       >  5:221275/1‑149 (MQ=255)
                     tGCACGGCCACGGCCAGCGCCGCACACACCAGGGCTGCCAGGTTGCCCATTTCGGGGCGGGCCAGTAGCGTCAGGGTCTTCCCAGCACGACGGCGACTACCAGCAGGGCGCAGGCCAGGTGCTGCAGTTGTTGCCGCGCGCCGCgatga                                                                                                                       <  1:118822/149‑1 (MQ=255)
                      gCACGGCCACGGCCAGCGCCGCACACACCAGGGCTGCCAGGTTGCCCATTTCGGGGCGGGCCAGTAGCGTCAGGGTCT‑‑GCAGCACGACGGCGACTACCAGCAGGGCGCAGGCCAGGTGCTGCAGTTGTTGCCGCGCGCCGCGATGATAc                                                                                                                    >  2:5099/1‑149 (MQ=255)
                        acggccacggccAGCGCCGCACACACCAGGGCTGCCAGGTTGCCCATTTCGGGGCGGGCCAGTAGCGTCAGGGTCT‑‑GCAGCACGACGGCGACTACCAGCAGGGCGCAGGCCAGGTGCTGCAGTTGTTGCCGCGCGCCGCGATGATACAg                                                                                                                  >  6:34672/1‑149 (MQ=255)
                          ggccacggccaGCGCCGCACACACCAGGGCTGCCAGGTTGCCCATTTCGGGGCGGGCCAGTAGCGTCAGGGTCT‑‑GCAGCACGACGGCGACTACCAGCAGGGCGCAGGCCAGGTGCTGCAGTTGTTGCCGCGCGCCGCGATGATACAGCg                                                                                                                >  6:130261/1‑149 (MQ=255)
                           gccacggccaGCGCCGCACACACCAGGGCTGCCAGGTTGCCCATTTCGGGGCGGGCCAGTAGCGTCAGGGTCT‑‑GCAGCACGACGGCGACTACCAGCAGGGCGCAGGCCAGGTGCTGCAGTTGTTGCCGCGCGCCGCGATGATACAGCgg                                                                                                               <  3:123839/149‑1 (MQ=255)
                            ccacggccaGCGCCGCACACACCAGGGCTGCCAGGTTGCCCATTTCGGGGCGGGCCAGTAGCGTCAGGGTCT‑‑GCAGCACGACGGCGACTACCAGCAGGGCGCAGGCCAGGTGCTGCAGTTGTTGCCGCGCGCCGCGATGATACAGCgg                                                                                                               >  5:135690/1‑148 (MQ=255)
                             cacggccaGCGCCGCACACACCAGGGCTGCCAGGTTGCCCATTTCGGGGCGGGCCAGTAGCGTCAGGGTCT‑‑GCAGCACGACGGCGACTACCAGCAGGGCGCAGGCCAGGTGCTGCAGTTGTTGCCGCGCGCCGCGATGATACAGCGGCa                                                                                                             >  4:119199/1‑149 (MQ=255)
                                ggccaGCGCCGCACACACCAGGGCTGCCAGGTTGCCCATTTCGGGGCGGGCCAGTAGCGTCAGGGTCT‑‑GCAGCACGACGGCGACTACCAGCAGGGCGCAGGCCAGGTGCTGCAGTTGTTGCCGCGCGCCGCGATGATACAGCGGCACGa                                                                                                          >  6:299681/1‑149 (MQ=255)
                                   caGCGCCGCACACACCAGGGCTGCCAGGTTGCCCATTTCGGGGCGGGCCAGTAGCGTCAGGGTCT‑‑GCAGCACGACGGCGACTACCAGCAGGGCGCAGGCCAGGTGCTGCAGTTGTTGCCGCGCGCCGCGATGATACAGCGGCACGACCg                                                                                                       >  8:95315/1‑149 (MQ=255)
                                    aGCGCCGCACACACCAGGGCTGCCAGGTTGCCCATTTCGGGGCGGGCCAGTAGCGTCAGGGTCT‑‑GCAGCACGACGGCGACTACCAGCAGGGCGCAGGCTAGGTGCTGCAGTTGTTGCCGCGCGCCGCGATGATACAGCGGCACGACCgg                                                                                                      <  1:282606/149‑1 (MQ=255)
                                     gcgcGGCACACACCTGGTCTGGCTGGTTGCCCATTTGGGGGCGGGCCAGTAGCGGCAGGGTCT‑‑GGAGCACGACGGCGTCTACCTGCAGGGCGCAGGCCAGGTGCTGCAGTTGTTGCCGCGCGCCGCGTTGATACAGCGGCACGACCggg                                                                                                     <  2:174884/149‑1 (MQ=255)
                                     gcgcCGCACACACCAGGGCTGCCAGGTTGCCCATTTCGGGGCGGGCCAGTAGCGTCAGGGTCT‑‑GCAGCACGACGGCGACTACCAGCAGGGCGCAGGCCAGGTGCTGCAGTTGTTGCCGCGCGCCGCGATGATACAGCGGCACGACCggg                                                                                                     <  3:23447/149‑1 (MQ=255)
                                         cgcACACGCCAGGGCTGCCAGGTTGCCCATTTCGGGGCGGGCCAGTAGCGTCAGGGTCT‑‑GCAGCACGACGGCGACTACCAGCAGGGCGCAGGCCAGGTGCTGCAGTTGTTGCCGCGCGCCGCGATGATACAGCGGCACGACCGGGGCCa                                                                                                 <  5:73293/149‑1 (MQ=255)
                                           cacacaCCAGGGCTGCCAGGTTGCCCATTTCGGGGCGGGCCAGTAGCGTCAGGGTCT‑‑GCAGCACGACGGCGACTACCAGCAGGGCGCAGGCCAGGTGCCGCAGTTGTTGCCGCGCGCCGCGATGATACAGCGGCACGACCGGGGCCAGc                                                                                               <  6:221275/149‑1 (MQ=255)
                                           cacacaCCAGGGCTGCCAGGTTGCCCATTTCGGGGCGGGCCAGTAGCGTCAGGGTCT‑‑GCAGCACGACGGCGACTACCAGCAGGGCGCAGGCCAGGTGCCGCAGTTGTTGCCGCGCGCCGCGATGATACAGCGGCACGACCGGGGCCAGc                                                                                               <  4:259813/149‑1 (MQ=255)
                                           cacacaCCAGGGCTGCCAGGTGGCCCATTTCGGGGCGGGCCAGTAGCGTCAGGGGCT‑‑GCAGCACGACGGCGACTACCAGCAGGGCGCAGGCCAGGTGCTGCAGTTGTTGCCGCGCGCCGCGATGATACAGCGGCACGACCGGGGCCAGc                                                                                               <  5:174186/149‑1 (MQ=255)
                                                acCAGGGCTGCCAGGTTGCCCATTTCGGGGCGGGCCAGTAGCGTCAGGGTCT‑‑GCAGCACGACGGCGACTACCAGCAGGGCGCAGGCCAGGTGCTGCAGTTGTTGCCGCGCGCCGCGATGATACAGCGGCACGACCGGGGCCAGCACAAg                                                                                          <  5:130261/149‑1 (MQ=255)
                                                acCAGGGCTGCCAGGTTGCCCATTTCGGGGCGGGCCAGTAGCGTCAGGGTCT‑‑GCAGCACGACGGCGACTACCAGCAGGGCGCAGGCCAGGTGCTGCAGTTGTTGCCGCGCGCCGCGATGATACAGCGGCACGACCGGGGCCAGCACAAg                                                                                          >  8:205741/1‑149 (MQ=255)
                                                acCAGGGCTGCCAGGTTGCCCATTTCGGGGCGGGCCAGTAGCGTCAGGGTCT‑‑GCAGCACGACGGCGACTACCAGCAGGGCGCAGGCCAGGTGCTGCAGTTGTTGCCGCGCGCCGCGATGATACAGCGGCACGACCGGGGCCAGCACAAg                                                                                          >  4:8743/1‑149 (MQ=255)
                                                acCAGGGCTGCCAGGTTGCCCATTTCGGGGCGGGCCAGTAGCGTCAGGGTCT‑‑GCAGCACGACGGCGACTACCAGCAGGGCGCAGGCCAGGTGCTGCAGTTGTTGCCGCGCGCCGCGATGATACAGCGGCACGACCGGGGCCAGCACAAg                                                                                          >  3:79649/1‑149 (MQ=255)
                                                acCAGGGCTGCCAGGTTGCCCATTTCGGGGCGGGCCAGTAGCGTCAGGGTCT‑‑GCAGCACGACGGCGACTACCAGCAGGGCGCAGGCCAGGTGCTGCAGTTGTTGCCGCGCGCCGCGATGATACAGCGGCACGACCGGGGCCAGCACAAg                                                                                          >  1:231317/1‑149 (MQ=255)
                                                acCAGGGCTGCCAGGTTGCCCATTTCGGGGCGGGCCAGTAGCGTCAGGGTCT‑‑GCAGCACGACGGCGACTACCAGCAGGGCGCAGGCCAGGTGCTGCAGTTGTTGCCGCGCGCCGCGATGATACAGCGGCACGACCGGGGCCAGCACAAg                                                                                          >  1:145457/1‑149 (MQ=255)
                                                  cAGGGCTGCCAGGTTGCCCATTTCGGGGCGGGCCAGTAGCGTCAGGGTCT‑‑GCAGCACGACGGCGACTACCAGCAGGGCGCAGGCCAGGTGCTGCAGTTGTTGCCGCGCGCCGCGATGATACAGCGGCACGACCGGGGCCAGCACAAGGt                                                                                        >  6:163550/1‑149 (MQ=255)
                                                   aGGGCTGCCAGGTTGCCCATTTCGGGGCGGGCCAGTAGCGTCAGGGTCT‑‑GCAGCACGACGGCGACTACCAGCAGGGCGCAGGCCAGGTGCTGCAGTTGTTGCCGCGCGCCGCGATGATACAGCGGCACGACCGGGGCCAGCACAAGGtt                                                                                       >  5:149102/1‑149 (MQ=255)
                                                     ggCTGCCAGGTTGCCCATTTCGGGGCGGGCCAGTAGCGTCAGGGTCT‑‑GCAGCACGACGGCGACTACCAGCAGGGCGCAGGCCAGGTGCTGCAGTTGTTGCCGCGCGCCGCGATGATACAGCGGCACGACCGGGGCCAGCACAAGGtt                                                                                       >  1:230201/1‑147 (MQ=255)
                                                       cTGCCAGGTTGCCCATTTCGGGGCGGGCCAATAGCGTCAGGGTCT‑‑GCAGCACGACGGCGACTACCAGCAGGGCGCAGGCCAGGTGCCGCAGTTGTTGCCGCGCGCCGCGATGATACAGCGGCACGACCGGGGCCAGCACAAGGTTGAGc                                                                                   <  3:195653/149‑1 (MQ=255)
                                                          ccAGGTTGCCCATTTCGGGGCGGGCCAGTAGCGTCAGGGTCT‑‑GCAGCACGACGGCGACTACCAGCAGGGCGCAGGCCAGGTGCTGCAGTTGTTGCCGCGCGCCGCGATGATACAGCGGCACGACCGGGGCCAGCACAAGGTTGAGCAgg                                                                                <  8:300776/149‑1 (MQ=255)
                                                          ccAGGTTGCCCATTTCGGGGCGGGCCAGTAGCGTCAGGGTCT‑‑GCAGCACGACGGCGACTACCAGCAGGGCGCAGGCCAGGTGCTGCAGTCGTTGCCGCGCGCCGCGATGATACAGCGGCACGACCGGGGCCAGCACAAGGTTGAGCAgg                                                                                >  6:228886/1‑149 (MQ=255)
                                                                   cgaTTTCGGTGCGGGGCAGTAGCGTCAGGGTCT‑‑GCAGCACGACGGCGACTACCAGCAGGGCGCAGGCCAGGTGCTGCAGTTGTTGCCGCGCGCCGCGATGATACAGCGGCACGACCGGGGCCAGCACAAGGTTGAGCAGGCCGAGCA‑gg                                                                      <  6:327103/147‑1 (MQ=255)
                                                                   ccATTTCGGGGCGGGCCAGTAGCGTCAGGGTCT‑‑GCAGCACGACGGCGACTACCAGCAGGGCGCAGGCCAGGTGCTGCAGTTGTTGCCGCGCGCCGCGATGATACAGCGGCACGACCGGGGCCAGCACAAGGTTGAGCAGGCCGAGCA‑gg                                                                      <  3:360541/149‑1 (MQ=255)
                                                                   ccATTTCGGGGCGGGCCAGTAGCGTCAGGGTCT‑‑GCAGCACGACGGCGACTACCAGCAGGGCGCAGGCCAGGTGCTGCAGTTGTTGCCGCGCGCCGCGATGATACAGCGGCACGACCGGGGCCAGCACAAGGTTGAGCAGGCCGAGCA‑gg                                                                      >  5:327103/1‑149 (MQ=255)
                                                                     aTTTCGGGGCGGGCCAGTAGCGTCAGGGTCT‑‑GCAGCACGACGGCGACTACCAGCAGGGCGCAGGCCAGGTGCTGCAGTTGTTGCCGCGCGCCGCGATGATACAGCGGCACGACCGGGGCCAGCACAAGGTTGAGCAGGCCGAGCA‑GGGc                                                                    >  8:75653/1‑149 (MQ=255)
                                                                      tttCGGGGCGGGCCAGTAGCGTCAGGGTCT‑‑GCAGCACGACGGCGACTACCAGCAGGGCGCAGGCCAGGTGCTGCAGTTGTTGCCGCGCGCCGCGATGATACAGCGGCACGACCGGGGCCAGCACAAGGTTGAGCAGGCCGAGCA‑GGGc                                                                    >  1:43589/1‑148 (MQ=255)
                                                                      tttCGGGGCGGGCCAGTAGCGTCAGGGTCT‑‑GCAGCACGACGGCGACTACCAGCAGGGCGCAGGCCAGGTGCTGCAGTTGTTGCCGCGCGCCGCGATGATACAGCGGCACGACCGGGGCCAGCACAAGGTTGAGCAGGCCGAGCA‑GGGc                                                                    >  1:49105/1‑148 (MQ=255)
                                                                        tCGGGGCGGGCCAGTAGCGTCAGGGTCT‑‑GCAGCACGACGGCGACTACCAGCAGGGCGCAGGCCAGGTGCTGCAGTTGTTGCCGCGCGCCACGATGATACAGCGGCACGACCGGGGCCAGCACAAGGTTGAGCAGGCCGAGCA‑GGGCCa                                                                  <  4:263912/148‑1 (MQ=255)
                                                                           gggcgggcCAGTAGCGTCAGGGTCT‑‑GCAGCACGACGGCGACTACCAGCAGGGCGCAGGCCAGGTGCTGCAGTTGTTGCCGCGCGCCGCGATGATACAGCGGCACGACCGGGGCCAGCACAAGGTTGAGCAGGCCGAGCA‑GGGCCAGGTa                                                              <  6:135690/149‑1 (MQ=255)
                                                                             gcgggcCAGTAGCGTCAGGGTCT‑‑GCAGCACGACGGCGACTACCAGCAGGGCGCAGGCCAGGTGCTGCAGTTGTTGCCGCGCGCCGCGATGATACAGCGGCACGACCGGGGCCAGCACAAGGTTGAGCAGGCCGAGCA‑GGGCCAGGTAGa                                                            <  7:205741/149‑1 (MQ=255)
                                                                                  ccAGTAGCGTCAGGGTCT‑‑GCAGCACGACGGCGACTACCAGCAGGGCGCAGGCCAGGTGCTGCAGTTGTTGCCGCGCGCCGCGATGATACAGCGGCACGACCGGGGCCAGCACAAGGTTGAGCAGGCCGAGCA‑GGGCCAGGTAGACcgcg                                                       <  2:145457/149‑1 (MQ=255)
                                                                                   cAGTAGCGTCAGGGTCT‑‑GCAGCACGACGGCGACTACCAGCAGGGCGCAGGCCAGGTGCTGCAGTTGTTGCCGCGCGCCGCGATGATACAGCGGCACGACCGGGGCCAGCACAAGGTTGAGCAGGCCGAGCACGCGTCACGTAGACcgcg                                                       >  1:131507/1‑149 (MQ=255)
                                                                                      tAGCGTCAGGGTCT‑‑GCAGCACGACGGCGACTACCAGCAGGGCGCAGGCCAGGTGCTGCAGTTGTTGCCGCGCGCCGCGATGATACAGCGGCACGACCGGGGCCAGCACAAGGTTGAGCAGGCCGAGCA‑GGGCCAGGTAGACCGCGTCgg                                                   <  7:95315/149‑1 (MQ=255)
                                                                                      tAGCGTCAGGGTCT‑‑GCAGCACGACGGCGACTACCAGCAGGGCGCAGGCCAGGTGCTGCAGTTGTTGCCGCGCGCCGCGATGATACAGCGGCACGACCGGGGCCAGCACAAGGTTGAGCAGGCCGAGCA‑GGGCCAGGTAGACCGCGTCgg                                                   >  2:76678/1‑149 (MQ=255)
                                                                                         cGTCAGGGTCT‑‑GCAGCACGACGGCGACTACCAGCAGGGCGCAGGCCAGGTGCTGCAGTTGTTGCCGCGCGCCGCGATGATACAGCGGCACGACCGGGGCCAGCACAAGGTTGAGCAGGCCGAGCA‑GGGCCAGGTAGACCGCGTCGGgtt                                                <  7:354541/149‑1 (MQ=255)
                                                                                           tCAGGGTCT‑‑GCAGCACGACGGCGACTACCAGCAGGGCGCAGGCCAGGTGCTGCAGTTGTTGCCGCGCGCCGCGATGATACAGCGGCACGACCGGGGCCAGCACAAGGTTGAGCAGGCCGAGCA‑GGGCCAGGTAGACCGCGTCGGgttgt                                              >  1:146403/1‑149 (MQ=255)
                                                                                           tCAGGGTCT‑‑GCAGCACGACGGCGACTACCAGCAGGGCGCAGGCCAGGTGCTGCAGTTGTTGCCGCGCGCCGCGATGATACAGCGGCACGACCGGGGCCAGCACAAGGTTGAGCAGGCCGAGCA‑GGGCCAGGTAGACCGCGTCGGgttgt                                              >  4:57939/1‑149 (MQ=255)
                                                                                              gggTCT‑‑GCAGCACGACGGCGACTACCAGCAGGGCGCAGGCCAGGTGCTGCAGTTGTTGCCGCGCGCCGCGATGATACAGCGGCACGACCGGGGCCAGCACAAGGTTGAGCAGGCCGAGCA‑GGGCCAGGTAGACCGCGTCGGGttgttg                                            >  8:11882/1‑148 (MQ=255)
                                                                                               ggTCT‑‑GCAGCACGACGGCGACTACCAGCAGGGCGCAGGCCAGGTGCTGCAGTTGTTGCCGCGCGCCGCGATGATACAGCGGCACGACCGGGGCCAGCACAAGGTTGAGCAGGCCGAGCA‑GGGCCAGGTAGACCGCGTCGGGTTGTTGCa                                          <  5:163550/149‑1 (MQ=255)
                                                                                                gTCT‑‑GCAGCACGACGGCGACTACCAGCAGGGCGCAGGCCAGGTGCTGCAGTTGTTGCCGCGCGCCGCGATGATACAGCGGCACGACCGGGGCCAGCACAAGGTTGAGCAGGCCGAGCA‑GGGCCAGGTAGACCGCGTCGGGTTGTTGCAg                                         <  2:170147/149‑1 (MQ=255)
                                                                                                  cT‑‑GCAGCACGACGGCGACTACCAGCAGGGCGCAGGCCAGGTGCTGCAGTTGTTGCCGCGCGCCGCGATGATACAGCGGCACGACCGGGGCCAGCACAAGGTTGAGCAGGCCGAGCA‑GGGCCAGGTAGACCGCGTCGGGTTGTTGCAgg                                        >  4:85726/1‑148 (MQ=255)
                                                                                                      gcagcaCGACGGCGACTACCAGCAGGGCGCAGGCCAGGTGCTGCAGTTGTTGCCGCGCGCCGCGATGATACAGCCGCACGACCGGGGCCAGCACAAGGTTGAGCAGGCCGAGCA‑GGGCCAGGTAGACCGCGTCGGGTTGTTGCAGGAAg                                     <  2:62785/149‑1 (MQ=255)
                                                                                                       cagcaCGACGGCGACTACCAGCAGGGCGCAGGCCAGGTGCTGCAGTTGTTGCCGCGCGCCGCGATGATACAGCGGCACGACCGGGGCCAGCACAAGGTTGAGCAGGCCGAGCA‑GGGCCAGGTAGACCGCGTCGGGTTGTTGCAGGa                                       <  2:230201/146‑1 (MQ=255)
                                                                                                       cagcaCGACGGCGACTACCAGCAGGGCGCAGGCCAGGTGCTGCAGTTGTTGCCGCGCGCCGCGATGATACAGCGGCACGACCGGGGCCAGCACAAGGTTGAGCAGGCCGAGCA‑GGGCCAGGTAGACCGCGTCGGGTTGTTGCAGGAAgg                                    <  4:79649/149‑1 (MQ=255)
                                                                                                        agcaCGACGGCGACTACCAGCAGGGCGCAGGCCAGGTGCTGCAGTTGTTGCCGCGCGCCGCGATGATACAGCGGCACGACCGGGGCCAGCACAAGGTTGAGCAGGCCGAGCA‑GGGCCAGGTAGACCGCGTCGGGTTGTTGGAGGAAggg                                   >  7:166871/1‑149 (MQ=255)
                                                                                                         gcaCGACGGCGACTACCAGCAGGGCGCAGGCCAGGTGCTGCAGTTGTTGCCGCGCGCCGCGATGATACAGCGGCACGACCGGGGCCAGCACAAGGTTGAGCAGGCCGAGCA‑GGGCCAGGTAGACCGCGTCGGGTTGTTGCAGGAAGGgt                                  >  4:180581/1‑149 (MQ=255)
                                                                                                         gcaCGACGGCGACTACCAGCAGGGCGCAGGCCAGGTGCTGCAGTTGTTGCCGCGCGCCGCGATGATACAGCGGCACGACCGGGGCCAGCACAAGGTTGAGCAGGCCGAGCA‑GGGCCAGGTAGACCGCGTCGGGTTGTTGCAGGAAGGgt                                  <  4:41685/149‑1 (MQ=255)
                                                                                                          caCGACGGCGACTACCAGCAGGGCGCAGGCCAGGTGCTGCAGTTGTTGCCGCGCGCCGCGATGATACAGCGGCACGACCGGGGCCAGCACAAGGTTGAGCAGGCCGAGCA‑GGGCCAGGTAGACCGCGTCGGgttgtt                                             >  8:259756/1‑137 (MQ=255)
                                                                                                          caCGACGGCGACTACCAGCAGGGCGCAGGCCAGGTGCTGCAGTTGTTGCCGCGCGCCGCGATGATACAGCGGCACGACCGGGGCCAGCACAAGGTTGAGCAGGCCGAGCA‑GGGCCAGGTAGACCGCGTCGGgttgtt                                             <  7:259756/137‑1 (MQ=255)
                                                                                                            cgacgGCGACTACCAGCAGGGCGCAGGCCAGGTGCTGCAGTTGTTGCCGCGCGCCGCGATGATACAGCGGCACGACCGGGGCCAGCACAAGGTTGAGCAGGCCGAGCA‑GGGCCAGGTAGACCGCGTCGGGTTGTTGCAGGAAGGGTGTc                               <  3:119199/149‑1 (MQ=255)
                                                                                                                ggCGACTACCAGCAGGGCGCAGGCCAGGTGCTGCAGTTGTTGCCGCGCGCCGCGATGATACAGCGGCACGACCGGGGCCAGCACAAGGTTGAGCAGGCCGAGCA‑GGGCCAGGTAGACCGCGTCGGGTTGTTGCAGGAAGGGTGTCGCAt                           >  3:216658/1‑149 (MQ=255)
                                                                                                                      tACCAGCAGGGCGCAGGCCAGGTGCTGCAGTTGTTGCCGCGCGCCGCGATGATACAGCGGCACGACCGGGGCCAGCACAAGGTTGAGCAGGCCGAGCA‑GGGCCAGGTAGACCGCGTCGGGTTGTTGCAGGAAGGGTGTCGCATCGGttt                     <  7:330579/149‑1 (MQ=255)
                                                                                                                        ccAGCAGGGCGCAGGCCAGGTGCTGCAGTTGTTGCCGCGCGCCGCGATGATACAGCGGCACGACCGGGGCCAGCACAAGGTTGAGCAGGCCGAGCA‑GGGCCAGGTAGACCGCGTCGGGTTGTTGCAGGAAGGGTGTCGCATCGGtttt                    >  1:332944/1‑148 (MQ=255)
                                                                                                                         cagcagGGCGCAGGCCAGGTGCTGCAGTTGTTGCCGCGCGCCGCGATGATACAGCGGCACGACCGGGGCCAGCACAAGGTTGAGCAGGCCGAGCA‑GGGCCAGGTAGACCGCGTCGGGTTGTTGCAGGAAGGGTGTCGCATCGGtttt                    >  7:112670/1‑147 (MQ=255)
                                                                                                                         cagcagGGCGCAGGCCAGGTGCTGCAGTTGTTGCCGCGCGCCGCGATGATACAGCGGCACGACCGGGGCCAGCACAAGGTTGAGCAGGCCGAGCA‑GGGCCAGGTAGACCGCGTCGGGTTGTTGCAGGAAGGGTGTCGCATCGGTTTTCa                  <  2:146403/149‑1 (MQ=255)
                                                                                                                               ggCGCAGGCCAGGTGCTGCAGTTGTTGCCGCGCGCCGCGATGATACAGCGGCACGACCGGGGCCAGCACAAGGTTGAGCAGGCCGAGCA‑GGGCCAGGTAGACCGCGTCGGGTTGTTGCAGGAAGGGTGTCGCATCGGTTTTCAGGCTgg            <  6:149102/149‑1 (MQ=255)
                                                                                                                               ggCGCAGGCCAGGTGCTGCAGTTGTTGCCGCGCGCCGCGATGATACAGCGGCACGACCGGGGCCAGCACAAGGTTGAGCAGGCCGAGCA‑GGGCCAGGTAGACCGCGTCGGGTTGTTGCAGGAAGGGTGTCGCATCGGTTTTCAGGCTgg            <  2:332944/149‑1 (MQ=255)
                                                                                                                                gcgcAGGCCAGGTGCTGCAGTTGTTGCCGCGCGCCGCGATGATACAGCGGCACGACCGGGGCCAGCACAAGGTTGAGCAGGCCGAGCA‑GGGCCAGGTAGACCGCGTCGGGTTGTTGCAGGAAGGGTGTCGCATCGGTTTTCAGGCTggg           <  5:34672/149‑1 (MQ=255)
                                                                                                                                gcgcAGGCCAGGTGCTGCAGTTGTTGCCGCGCGCCGCGATGATACAGCGGCACGACCGGGGCCAGCACAAGGTTGAGCAGGCCGAGCA‑GGGCCAGGTAGACCGCGTCGGGTTGGTGCAGGAAGGGTGTCGCATCGGTTTTCAGGCTggg           >  2:222762/1‑149 (MQ=255)
                                                                                                                                 cgcAGGCCAGGTGCTGCAGTTGTTGCCGCGCGCCGCGATGATACAGCGGCACGACCGGGGCCAGCACAAGGTTGAGCAGGCCGAGCA‑GGGCCAGGTAGACCGCGTCGGGTTGTTGCAGGAAGGGTGTCGCATCGGTTTTCAGGCTggg           <  5:296787/148‑1 (MQ=255)
                                                                                                                                 cgcAGGCCAGGTGCTGCAGTTGTTGCCGCGCGCCGCGATGATACAGCGGCACGACCGGGGCCAGCACAAGGTTGAGCAGGCCGAGCA‑GGGCCAGGTAGACCGCGTCGGGTTGTTGCAGGAAGGGTGTCGCATCGGTTTTCAGGCTggg           >  6:296787/1‑148 (MQ=255)
                                                                                                                                 cgcAGGCCAGGTGCTGCAGTTGTTGCCGCGCGCCGCGATGATACAGCGGCACGACCGGGGCCAGCACAAGGTTGAGCAGGCCGAGCA‑GGGCCAGGTAGACCGCGTCGGGTTGTTGCAGGAAGGGTGTCGCATCGGTTTTCAGGCTGGGt          <  3:8743/149‑1 (MQ=255)
                                                                                                                                  gcAGGCCAGGTGCTGCAGTTGTTGCCGCGCGCCGCGATGATACAGCGGCACGACCGGGGCCAGCACAAGGTTGAGCAGGCCGAGCA‑GGGCCAGGTAGACCGCGTCGGGTTGTTGCAGGAAGGGTGTCGCATCGGTTTTCAGGCTGGGTa         >  6:196851/1‑149 (MQ=255)
                                                                                                                                     ggCCAGGTGCTGCAGTTGTTGTCGCGCGCCGCGATGATACAGCGGCACGACCGGGGCCAGCACAAGGTTGAGCAGGCCGAGCA‑GGGCCAGGTAGACCGCGTCGGGTTGTTGCAGGAAGGGTGTCGCATCGGTTTTCAGGCTGGGTATGa      <  3:63244/149‑1 (MQ=255)
                                                                                                                                         aGGTGCTGCAGTTGTTGCCGCGCGCCGCGATGATACAGCGGCACGACCGGGGCCAGCACAAGGTTGAGCAGGCCGAGCA‑GGGCCAGGTAGACCGCGTCGGGTTGTTGCAGGAAGGGTGTCGCATCGGTTTTCAGGCTGGGTATGAGCGa  <  2:231317/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                               
CGGGCAAAGCCCACGGCCAGATGCACGGCCACGGCCAGCGCCGCACACACCAGGGCTGCCAGGTTGCCCATTTCGGGGCGGGCCAGTAGCGTCAGGGTCT‑‑GCAGCACGACGGCGACTACCAGCAGGGCGCAGGCCAGGTGCTGCAGTTGTTGCCGCGCGCCGCGATGATACAGCGGCACGACCGGGGCCAGCACAAGGTTGAGCAGGCCGAGCA‑GGGCCAGGTAGACCGCGTCGGGTTGTTGCAGGAAGGGTGTCGCATCGGTTTTCAGGCTGGGTATGAGCGA  >  NC_002947/1388158‑1388441

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑