Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,449,139	T→C	9.2%	*287R (TGA→CGA) ‡	PP_1266 →	fusaric acid resistance protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,449,139	0	T	C	9.2%	176.5 / 7.5	65	*287R (TGA→CGA) ‡	PP_1266	fusaric acid resistance protein
Reads supporting (aligned to +/- strand):  ref base T (28/31);  new base C (4/2);  total (32/33)
Fisher's exact test for biased strand distribution p-value = 4.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.12e-01

CGAGCTGATCGCCGACGTGAACCCGACCTTCAACTGGGTGCGGCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTGGTCCCAATCGTCCGGTGGGTTGCCGCTATGCAACATCTTTT  >  NC_002947/1448991‑1449284                                                                                                                                                     |                                                                                                                                                  cgAGCTGATCGCCGACGTGAACCCGACCTTCAACTGGGTGCGGCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGtt                                                                                                                                                   <  5:345055/149‑1 (MQ=255)   aGCTGATCGCCGACGTGAACCCGACCTTCAACTGGGTGCGGCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGa                                                                                                                                                 >  3:112362/1‑149 (MQ=255)      tGATCGCCGACGTGAACCCGACCTTCAACTGGGTGCGGCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCt                                                                                                                                              >  3:105431/1‑149 (MQ=255)        aTCGCCGACGTGAACCCGACCTTCAACAGGGAGCGGCTCGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTc                                                                                                                                            >  3:100182/1‑149 (MQ=255)           gCCGACGTGAACCCGACCTTCAACTGGGTGCGGCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTcgc                                                                                                                                          <  5:199579/148‑1 (MQ=255)           gCCGACGTGAACCCGACCTTCAACTGGGTGCGGCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTcgc                                                                                                                                          <  5:240859/148‑1 (MQ=255)            ccGACGTGAACCCGACCTTCAACTGGGTGCGGCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGACGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTCAACCTTcgcca                                                                                                                                        <  4:114437/149‑3 (MQ=255)            ccGACGTGAACCCGACCTTCAACTGGGTGCGGCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGa                                                                                                                                        <  7:110787/149‑1 (MQ=255)            ccGACGTGAACCCGACCTTCAACTGGGTGCGGCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTCGCGAAGGTCAACCTTcgcca                                                                                                                                        <  6:5764/149‑3 (MQ=255)            ccGACGTGAACCCGACCTTCAACTGGGTGCGGCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGATTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGa                                                                                                                                        >  7:239526/1‑149 (MQ=255)             cGACTTTAACCCGACCTTCAACTGGGAGCGGCTCGCACAGCGGGTCCCGGTGCGCATTCACATAGATGAAGTGCCGGAAGCGTATTTGCTGGCGGCGGGGACGACTAGTACGGAGGTAGTCAACCCTGGCGAAGCTTGACCTTCGCGAc                                                                                                                                       >  7:245269/1‑149 (MQ=255)                      cccGACCTTCAACTGGGTGCGGCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGc                                                                                                                              >  5:161944/1‑149 (MQ=255)                      cccGACCTTCAACTGGGTGCGGCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGc                                                                                                                              >  3:18130/1‑149 (MQ=255)                      cccGACCTTCAACTGGGTGCGGCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGc                                                                                                                              <  2:212603/149‑1 (MQ=255)                                  cTGGGTGCGGCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGGTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTGCGCGACCATCCCGGCCTCCCGcatcat                                                                                                                  <  6:274387/149‑1 (MQ=255)                                   tGGGTGCGGCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCatcatc                                                                                                                 >  6:105688/1‑149 (MQ=255)                                   tGGGTGCGGCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCatcatc                                                                                                                 >  6:2839/1‑149 (MQ=255)                                         cGGCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAATGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTggg                                                                                                           <  1:342413/149‑1 (MQ=255)                                          ggCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTgg                                                                                                            >  4:160728/1‑147 (MQ=255)                                           gCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCg                                                                                                                                >  3:334246/1‑126 (MQ=255)                                           gCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCg                                                                                                                                <  4:334246/126‑1 (MQ=255)                                             tGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGcc                                                                                                       <  1:346555/149‑1 (MQ=255)                                                 acaGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCctgc                                                                                                   <  8:19855/149‑1 (MQ=255)                                                   aGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGTCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCACGGCCTCCCGCATCATCTGTGGGAGCCatgct                                                                                                  >  7:278605/1‑148 (MQ=255)                                                      gggTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGatg                                                                                              <  4:368769/149‑1 (MQ=255)                                                       ggTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGatgc                                                                                             <  1:216513/149‑1 (MQ=255)                                                             ggTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCTCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGt                                                                                       <  4:100182/149‑1 (MQ=255)                                                                 cgcTTTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGTGGACGACGTGTACGGTGGTAGTGAAGCCTGGCGAAGGTTGAGCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTAttt                                                                                   <  6:311641/149‑2 (MQ=255)                                                                 cgcATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTAt                                                                                     >  4:54317/1‑147 (MQ=255)                                                                      tCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAg                                                                              >  6:50950/1‑149 (MQ=255)                                                                        acaTCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTc                                                                            <  4:366381/149‑1 (MQ=255)                                                                          aTCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCgg                                                                          >  4:258579/1‑149 (MQ=255)                                                                           tCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGc                                                                         <  1:106200/149‑1 (MQ=255)                                                                             gttGATGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTTCGTTGGTAGTCAAGCGTGGGGAAGGTTGACCTTCGCGACCATCCCGGCCTCGCGCATCATCTGTGGGAGCCCTGCTGTTGCATGCGTATTAGCCAGTGGGCTc                                                                       <  8:245269/147‑1 (MQ=255)                                                                             gATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCt                                                                        <  8:79197/148‑1 (MQ=255)                                                                              aTGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGat                                                                                               >  3:191456/1‑124 (MQ=255)                                                                              aTGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGat                                                                                               <  4:191456/124‑1 (MQ=255)                                                                                   gTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTg                                                                 >  6:158751/1‑149 (MQ=255)                                                                                    tGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGc                                                                <  4:112362/149‑1 (MQ=255)                                                                                      ccGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCg                                                               <  6:161944/148‑1 (MQ=255)                                                                                        ggAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGTGTATTAGCCAGTCGGCTCAATCTGCgg                                                              >  5:193296/1‑147 (MQ=255)                                                                                        ggAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCgg                                                              >  5:35291/1‑147 (MQ=255)                                                                                               tttttGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGTGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCtt                                                     <  6:193296/149‑1 (MQ=255)                                                                                                       ggcggcGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAAc                                             >  1:361599/1‑149 (MQ=255)                                                                                                         cggcggGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTg                                           <  5:158751/149‑1 (MQ=255)                                                                                                           gcggGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTGGt                                         <  3:54317/149‑1 (MQ=255)                                                                                                             ggggACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTGGTcc                                       >  2:201795/1‑149 (MQ=255)                                                                                                                           cGGTGGGAGTCACGCCTGGCGAAGGGTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTGGTCCCAATCGTCCGGTgg                         <  8:239526/149‑1 (MQ=255)                                                                                                                               ggtAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCAACATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTGGTCCCAATCGTCCGGTGGGTTg                     >  1:287406/1‑149 (MQ=255)                                                                                                                                 tAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTGGTCCCAATCGTCCGGTGGGTTGcc                   <  5:50950/149‑1 (MQ=255)                                                                                                                                   gTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTGGTCCCAATca                                 <  5:246201/133‑2 (MQ=255)                                                                                                                                   gTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTGGTCCCAATca                                 >  6:246201/1‑132 (MQ=255)                                                                                                                                    tCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTGGTCCCAATCGTCCGGTGGGTTGc                    >  6:55108/1‑145 (MQ=255)                                                                                                                                    tCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTGGTCCCAATCGTCCGGTGGGTTGc                    <  5:55108/145‑1 (MQ=255)                                                                                                                                       aGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTGGTCCCAATCGTCCGGTGGGTTGCCGCTATg             <  2:361599/149‑1 (MQ=255)                                                                                                                                         ccTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTGGTCCCAATCGTCCGGTGGGTTGCCGCTATGc            >  8:34967/1‑148 (MQ=255)                                                                                                                                          gtcgCGAAGGTCAACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCACCTGGTCCCAATCGTCCGGTGGGGTGCCGCTATGCaa          >  4:214970/4‑149 (MQ=255)                                                                                                                                          gtcgCGAAGGTCAACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTGGTCCCAATCGTCCGGTGGGTTGCCGCTATGCaa          >  8:248986/4‑149 (MQ=255)                                                                                                                                          cTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTGGTCCCAATCGTCCGGTGGGTTGCCGCTATGCaa          <  4:18130/149‑1 (MQ=255)                                                                                                                                             gCGAAGGTCAACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTGGTCCCAATCGTCCGGTGGGTTGCCGCTATGCAACAt       >  5:354309/1‑149 (MQ=255)                                                                                                                                             gCGAAGGTCAACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCCCAATCTGCGGCAGCCTCTTGCAGCAACTGGTCCCAATCGTCCGGTGGGTTGCCGCTATGCAACAt       >  6:27730/1‑149 (MQ=255)                                                                                                                                               gAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTGGTCCCAATCGTCCGGTGGGTTGCCGCTATGCAACATCt     >  2:229128/1‑149 (MQ=255)                                                                                                                                                 aGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTGGTCCCAATCGTCCGGTGGGTTGCCGCTATGCAACATCttt   >  8:71058/1‑149 (MQ=255)                                                                                                                                                  ggTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTGGTCCCAATCGTCCGGTGGGTTGCCGCTATGCAACATCtttt  <  6:35291/149‑1 (MQ=255)                                                                                                                                                  ggTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTGGTCCCAATCGTCCGGTGGGTTGCCGCTATGCAACATCtttt  <  5:105688/149‑1 (MQ=255)                                                                                                                                                   gTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTGGTCCCAATCGTCCGGTGGGTTGCCGCTATGCAACATCtttt  >  1:380944/1‑148 (MQ=255)                                                                                                                                                     |                                                                                                                                                  CGAGCTGATCGCCGACGTGAACCCGACCTTCAACTGGGTGCGGCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTGGTCCCAATCGTCCGGTGGGTTGCCGCTATGCAACATCTTTT  >  NC_002947/1448991‑1449284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑