Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,450,561 G→A 9.5% intergenic (‑18/‑256) PP_1269 ← / → PP_1270 universal stress protein family protein/LysR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,450,5610GA 9.5% 114.2 / 2.2 43intergenic (‑18/‑256)PP_1269/PP_1270universal stress protein family protein/LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (19/19);  new base A (2/2);  total (21/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTGGAATCGATGGCCAGCAGCACGTGCAGTTCGTGTTCGCCGGGGCGGCAGTAGCGGCGGGCGAGGGTGAGCAGGGTGCTGGAGGCGGGGGAGGCGTCGATGGCGATCAGGACGGGGCTGGGCATTGGAATCCTTGGCAGAGGCTGGCTTGTGTGGCTGCAGGATCGGCCCTTTCGCGGGCATGCCCGCTCCCACAGGTGTGTCGCACAACATTCGGGAATTGTGGGGGGGCCTGTGGCAGCGGGCGCGCCCGCGAAAAGGCCAGACCTGCCAATGCTTCATTTAAG  >  NC_002947/1450418‑1450705
                                                                                                                                               |                                                                                                                                                
ggTGGAATCGATGGCCAGCAGCACGTGCAGTTCGTGTTCGCCGGGGCGGCAGTAGCGGCGGGCGAGGGTGAGCAGGGTGCTGGAGGCGGGGGAGGCGTCGATGGCGATCAGGACGGGGCTGGGCATTGGAATCCTTGGCAGAggctggc                                                                                                                                             >  1:207406/1‑149 (MQ=255)
 gTGGAATCGATGGCCAGCAGCACGTGCAGTTCGTGTTCGCCGGGGCGGCAGTAGCGGCGGGCGAGGGTGAGCAGGGTGCTGGAGGCGCGGGAGGCGTCGATGGCGATCAGGACGGGGCTGGGCATTGGAATCCTTGGCAGAggctggct                                                                                                                                            >  5:245196/1‑149 (MQ=255)
      aTCGATGGCCAGCAGCACGTGCAGTTCGTGTTCGCCGGGGCGGCAGTAGCGGCGGGCGAGGGTGAGCAGGGTGCTGGAGGCGGGGGTGGCGTCGATGGCGATCAGGTCGGGGCTGGGCATTGGAATCCTTGGCAGAGGCTGGCTtgtgt                                                                                                                                       <  8:253348/149‑1 (MQ=255)
      aTCGATGGCCAGCAGCACGTGCAGTTCGTGTTCGCCGGGGCGGCAGTAGCGGCGGGCGAGGGTGAGCAGGGTGCTGGAGGCGGGGGAGGCGTCGATGGCGATCAGGACGGGGCTGGGCATTGGAATCCTTGGCAGAGGCTGGCTtgtgt                                                                                                                                       <  3:45476/149‑1 (MQ=255)
         gATGGCCAGCAGCACGTGCAGTTCGTGTTCGCCGGGGCGGCAGTAGCGGCGGGCGAGGGTGAGCAGGGTGCTGGAGGCGGGGGAGGCGTCGATGGCGATCAGGACGGGGCTGGGCATTGGAATCCTTGGCAGAGGCTGGCTTGTGTgg                                                                                                                                     <  8:101465/148‑1 (MQ=255)
                      aCGTGCTGTTCGTTTTGCCCGGGGCGGCAGTAGCTGCGGGCGAGGGTGTGCAGGGTGCTGGAGGCGGGGGTGGCGTCGATGGCGTTCAGGACGGGGCTGGGCATTGGAATCCTTGGCAGAGGCTGGCTTGTGTGGCTGCa                                                                                                                                <  7:269344/140‑1 (MQ=255)
                      aCGGGCAGTTCGTGTTCGCCGGGCCGGCAGTAGCGGCGGCCGAGGGTGAGCAGGGTCCTGCAGGCGGCGGAGCCGTCGATGGCGATCAGGACGGGGCTGGGCATTGGAAAGCGTGGCAGCGGCTGGCTTGTGTGGCTGCAgagatctg                                                                                                                        >  8:269344/1‑141 (MQ=255)
                        gTGCAGTTCGTGTTCGCCGGGGCGGCAGTAGCGGCGGGCGAGGGTGAGCAGGGTGCTGGAGGCGGGGGAGGCGTCGATGGCGATCAGGACGGGGCTGGGCATTGGAATCCTTGGCAGAGGCTGGCTTGTGTGGCTGCAGGATCGGCCCt                                                                                                                     >  3:112025/1‑149 (MQ=255)
                                       gCCGGGGCGGCAGTAGCGGCGGGCGAGGGTGAGCAGGGTGCTGGAGGCGGGGGAGGCGTCGATGGCGATCAGGACGGGGCTGGGCATTGGAATCCTTGGCAGAGGCTGGCTTGTGTGGCTGCAGGATCGGCCCTTTCGCGGGCATGccc                                                                                                      >  5:126654/1‑149 (MQ=255)
                                       gCCGGGGCGGCAGTAGCGGCGGGCGAGGGTGAGCAGGGTGCTGGAGGCGGGGGAGGCGTCGATGGCGATCAGGACGGGGCTGGGCATTGGAATCCTTGGCAGAGGCTGGCTTGTGTGGCTGCAGGATCGGCCCTTTCGCGGGCATGccc                                                                                                      >  3:202592/1‑149 (MQ=255)
                                        ccGGGGCGGCAGTAGCGGCGGGCGAGGGTGAGCAGGGTGCTGGAGGCGGGGGAGGCGTCGATGGCGATCAGGACGGGGCTGGGCATTGGAATCCTTGGCAGAGGCTGGCTTGTGTGGCTGCAGGATCGGCCCTTTCGCGGGCATGCCCg                                                                                                     >  2:148281/1‑149 (MQ=255)
                                         cGGGGCGGCAGTAGCGGCGGGCGAGGGTGAGCAGGGTGCTGGAGGCGGGGGAGGCGTCGATGGCGATCAGGACGGGGCGGGGCATTGGAATCCTTGGCAGAGGCTGGCTTGTGTGGCTGCAGGATCGGCCCTTTCGCGGGCATGCCCGc                                                                                                    <  7:272029/149‑1 (MQ=255)
                                          ggggCGGCAGTAGCGGCGGGCGAGGGTGAGCAGGGTGCTGGAGGCGGGGGAGGCGTCGATGGCGATCAGGACGGGGCTGGGCATTGGAATCCTTGGCAGAGACTGGCTTGTGTGGCTGCAGGATCGGCCCTTTCGCGGGCATGCCCGCt                                                                                                   >  5:335844/1‑149 (MQ=255)
                                            ggcggcTGTAGCGGCGGGCGAGGGTGAGCAGGGTGGTGGAGGCGGGGGAGGCGTCGATGGCGATCAGGACGGGGCTGGGAATTGGAATCCTTGGCAGAGGCTGGCTTGTGTGGCTTCAGGATCGGCCCTTTCGCGGGCATGCCCGcgaa                                                                                                 <  1:327595/149‑4 (MQ=255)
                                                 cAGTAGCGGCGGGCGAGGGTGAGCAGGGTGCTGGAGGCGGGGGAGGCGTCGATGGCGATCAGGACGGGGCTGGGCATTGGAATCCTTGGCAGAGTCTGGCTTGTGTGGCTGCAGGATCGGCCCTTTCTCGGGCATGCCCGCTCCCACAg                                                                                            <  2:207406/149‑1 (MQ=255)
                                                 cAGTAGCGGCGGGCGAGGGTGAGCAGGGTGCTGGAGGCGGGGGAGGCGTCGATGGCGATCAGGACGGGGCTGGGCATTGGAATCCTTGGCAGAGGCTGGCTTGTGTGGCTGCAGGATCGGCCCTTTCGCGGGCATGCCCGCTCCCACAg                                                                                            <  5:142492/149‑1 (MQ=255)
                                                        ggcgtgcgAGGGTGAGCAGGCGGCTGTAGGCGGGGGTGGCTTCGATGGCGATCAGGACGGGGCTGGGCATTGTAATCCTTGGCAGAGGCTGGGTTGTGTGGCTGCAGGATCGGCCCTTTCGCGGGCATGCCCGCTCCCACAGGTGTGTc                                                                                     <  6:28291/149‑1 (MQ=255)
                                                           gggcgAGGGTGAGCAGGGTGCTGGAGGCGGGGGAGGCGTCGATGGCGATCAGGACGGGGCTGGGCATTGGAATCCTTGGCAGAGGCTGGCTTGTGTGGCTGCAGGATCGGCCCTTTCGCGGGCATGCCCGCTCCCACAGGTGTGTCGca                                                                                  <  1:10519/149‑1 (MQ=255)
                                                           gggcgAGGGTGAGCAGGCTGCTGGAGGCGGGGGACGCGTCGATGGCGAGCAGGACGCGGCGGGGCATTGGAATCCTTGGCAGAGGCTGGCTTGTGTGGCTGCAGGATCGGCCCTATCGCGGGCATGCCCGCTCCCACAGGTGTGGCGca                                                                                  <  4:112025/149‑1 (MQ=255)
                                                                     gAGCAGGGTGCTGGAGGCGGGGGAGGCGTCGATGGCGATCAGGACGGGGCTGGGCATTGGAATCCTTGGCAGAGGCTGGCTTGTGTGGCTGCAGGATCGGCCCTTTCGCGGGCATGCCCGCTCCCACAGGTGTGTCGCACAACATTCgg                                                                        <  6:126654/149‑1 (MQ=255)
                                                                           ggTGCTGGAGGCGGGGGAGGCGTCGATGGCGATCAGGACGGGGATGGGGATTGGAATACTTGGCAGAGGCTGGCTTGTGTGGCTGCAGGATCGGCCCTTTCGCGGGCATGCCCGCTCCCACAGGTGTGTCGCACTACATTCGGGAATtg                                                                  >  7:328416/1‑149 (MQ=255)
                                                                            gTGCTGGAGGCGGGGGAGGCGTCGATGGCGATCAGGACGGGGCTGGGCATTGGAATCCTTGGCAGAGGCTGGCTTGTGTGGCTGCAGGATCGGCCCTTTCGCGGGCATGCCCGCTCCCACAGGTGTGTCGCACAACATTCgg                                                                        <  7:63039/142‑1 (MQ=255)
                                                                            gTGCTGGAGGCGGGGGAGGCGTCGATGGCGATCAGGACGGGGCTGGGCATTGGAATCCTTGGCAGAGGCTGGCTTGTGTGGCTGCAGGATCGGCCCTTTCGCGGGCATGCCCGCTCCCACAGGTGTGTCGCACAACATTCgg                                                                        >  8:63039/1‑142 (MQ=255)
                                                                                    ggCGGGGGAGGCGTCGATGGCGATCAGGACGGGGCTGGGCATTGGAATCCTTGGCAGAGGCTGGCTTGTGTGGCTGCAGGATCGGCCCTTTCGCGGGCATGCCCGCTCCCACAGGTGTGTCg                                                                                    <  1:59600/122‑1 (MQ=255)
                                                                                    ggCGGGGGAGGCGTCGATGGCGATCAGGACGGGGCTGGGCATTGGAATCCTTGGCAGAGGCTGGCTTGTGTGGCTGCAGGATCGGCCCTTTCGCGGGCATGCCCGCTCCCACAGGTGTGTCg                                                                                    >  2:59600/1‑122 (MQ=255)
                                                                                    ggCGGGGGAGGCGTCGAGGTCGATCAGGACGGGGCCGGGCATTGGATTCCTCGGCAGAGGCTCGCTCGTGTGGCTGCAGGATCGGCCCTTTCGCGGGCATGCCCGCTCCCACAGGGGTGTCGCACAACATTCGGGAATTGTGGGGGGGc                                                         <  6:245196/149‑1 (MQ=38)
                                                                                          ggAGGCGTCGATGGCGATCAGGACGGGGCTGGGCATTGGAATCCTTGGCAGAGGCTGGCTTGTGTGGCTGCAGGATCGGCCCTTTCGCGGGCATGCCCGCTCCCACAGGTGTGTCGCACAACATTCGGGAATTGTGGGGGGGCCTGTgg                                                   >  5:178319/1‑149 (MQ=255)
                                                                                           gAGGCGTCGATGGCGATCAGGACGGGGCTGGGCATTGGAATCCTTGGCAGAGACTGGCTTGTGTGGCTGCAGGATCGGCCCTTTCGCGGGCATGCCCGCTCCCACAGGTGTGTCGCACAACATTCGGGAATTGTGGGGGGGCCTGTgg                                                   >  8:38469/1‑148 (MQ=255)
                                                                                               cGTCGATGGCGATCAGGACGGGGCTGGGCATTGGAATCCTTGGCAGAGGCTGGCTTGTGTGGCTGCAGGATCGGCCCTTTCGCGGGCATGCCCGCTCCCACAGGTGTGTCGCACAACATTCGGGAATTGTGGGGGGGCCTGTGGCAGCg                                              >  3:186610/1‑149 (MQ=255)
                                                                                                    aTGGCGATCAGGACGGGGCTGGGCATTGGAATCCTTGGCAGAGGCTGGCTTGTGTGGCTGCAGGATCGGCCCTTTCGCGGGCATGCCCGCTCCCACAGGTGTGTCGCACAACATTCGGGAATTGTGGGGGGGCCTGTGGCCGCGGgcgc                                         >  3:294053/1‑149 (MQ=255)
                                                                                                        cgtgtcggACGGGGCGGGGCATTGGAATCCTTGGCAGAGACTGGCTTGTGGGGCTGCAGGTTCGGCCCTTTCGCGGGCATGCCCGCTCCCACAGGTGTGTCGCACAACATTCTGGAATTGTGGGGGGGCCTGTGGCAGCGGGCGCGccc                                     <  6:335844/143‑1 (MQ=255)
                                                                                                         gATCAGGACGGGGCTGGGCATTGGAATCCTTGGCAGAGGCTGGCTTGTGTGGCTGCAGGATCGGCCCTTTCGCGGGCATGCCCGCTCCCACAGGTGTGTCGCACAACATTCGGGAATTGTGGGGGGGCCTGTGGCAGCGGGCGCGCCcg                                    >  4:119168/1‑149 (MQ=255)
                                                                                                          aTCAGGACGGGGCTGGGCATTGGAATCCTTGGCAGAGGCTGGCTTGTGTGGCTGCAGGATCGGCCCTTTCGCGGGCATGCCCGCTCCCACAGGTGTGTCGCACAACATTCGGGAATTGTGGGGGGGCCTGTGGCAGCGGGCGCGCCcgc                                   >  6:99563/1‑149 (MQ=255)
                                                                                                               gACGGGGCTGGGCATTGGAATCCTTGGCAGAGGCTGGCTTGTGTGGCTGCAGGATCGGCCCTTTCGCGGGCATGCCCGCTCCCACAGGTGTGTCGCACAACATTCGGGAATTGTGGGGGGGCCTGTGGCAGCGGGCGCGCCCGCGaaaa                              >  4:178242/1‑149 (MQ=255)
                                                                                                                    ggCTGGGCATTGGAATCCTTGGCAGAGGCTGGCTTGTGTGGCTGCAGGATCGGCCCTTTCGCGGGCATGCCCGCTCCCACAGGTGTGTCGCACAACATTCGGGAATTGTGGGGGGGCCTGTGGCAGCGGGCGCGCCCGCGAAAAGTccc                         >  7:318438/1‑148 (MQ=255)
                                                                                                                    ggCTGGGCATTGGAATCCTTGGCAGAGGCTGGCTTGTGTGGCTGCAGGATCGGCCCTTTCGCGGGCATGCCCGCTCCCACAGGTGTGTCGCACAACATTCGGGAATTGTGGGGGGGCCTGTGGCAGCGGGCGCGCCCGCGAAAAGGc                           >  2:91821/1‑147 (MQ=255)
                                                                                                                         ggCTTTGTAATCCTTGGCCGCGGCTGGCTTGTGTGGCTGCAGGATCGGCCCTTTCGCGGGCAGGCCCGCTCCCACCGGGGTGTCGCACAACATTCGGTAATTGTGGGGGGGCCTGTGGCAGCGGGCGCGCCCGCGAAAAGGCCAGAc                      <  2:183276/147‑1 (MQ=255)
                                                                                                                         ggCATTGGAATCCTTGGCAGAGGCTGGCTTGTGTGGCTGCAGGATCGGCCCTTTCGCGGGCATGCCCGCTCCCACAGGTGTGTCGCACAACATTCGGGAATTGTGGGGGGGCCTGTGGCAGCGGGCGCGCCCGCGAAAAGGCCAGACCt                    >  7:228471/1‑149 (MQ=255)
                                                                                                                                gAATCCTTGGCAGAGGCTGGCTTGTGTGGCTGCAGGATCGGCCCTTTCGCGGGCATGCCCGCTCCCACAGGTGTGTCGCACAACATTCGGGAATTGTGGGGGGGCCTGTGGCAGCggg                                            <  2:132836/118‑1 (MQ=255)
                                                                                                                                   tCCTTCGCCGCGGCTGGCGTGTGGCGCTGCAGCATCGGCCCTTTCGCGGGCATGCCCGCTCCCACAGGTGTGTCCCACAACATTCGGGAATGGGGGGGGGGCCTGTGGCAGCGGGCGCGCCCGCGAAAAGGCCAGACCTGCCAATGCtt          <  1:275581/149‑1 (MQ=255)
                                                                                                                                     cTTGGCAGAGGCTGGCTTGTGTGGCTGCAGGATCGGCCCTTTCGCGGGCATGCCCGCTCCCACAGGTGTGTCGAACAACATTCGGGAATTGTGGGGGGGCCTGTGGCAGCGGGCGCGCCCGCCAAAAGGCCAGACCTGCCAATGCTTCa        >  1:333255/1‑149 (MQ=255)
                                                                                                                                        ggCAGAGACTGGCTTGTGTGGCTGCAGGATCGGCCCTTTCGCGGGCATGCCCGCTCCCACAGGTGTGTCGCACAACATTCGGGAATTGTGGGGGGGCCTGTGGCAGCGGGCGCGCCCGCGAAAAGGCCAGACCTGCCAATGCTTCAt       <  7:38469/147‑1 (MQ=255)
                                                                                                                                         gCAGAGGCTGGGTTGTGTGGCTTCAGGATCGGCCCTTTTGAGGGCATGCGCGCTCCCACTGTTGTGTCGCACAACATTCGGGATTTGGGGGGGGGGCTGTGGCAGCGGGCGCGCCCGCGAAAAGGCCAGACCTGCCAATGCTTCATTTa    <  2:333255/149‑1 (MQ=255)
                                                                                                                                          cAGAGGCTGGCTTGTGTGGCTGCAGGATCGGCCCTTTCGCGGGCATGCCCGCTCCCACAGGTGTGTCGCACAACATTCGGGAATTGTGGGGGGGCCTGTGGCAGCGGGCGCGCCCGCGAAAAGGCCAGACCTGCCAATGCTTCAttt     >  3:352776/1‑147 (MQ=255)
                                                                                                                                           agagGCTGGCTTGTGTGGCTGCAGGATCGGCACTTTCGCGGGCATGCCCGCTCCCACAGGTGTGTCGCACAACATTCGGTAATTGTGGGGGGGCCTGTGGCAGCGGGCGCGCCCGCGAAAAGGCCAGACCTGCCAATGCTTCATTTAAg  <  4:202592/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                
GGTGGAATCGATGGCCAGCAGCACGTGCAGTTCGTGTTCGCCGGGGCGGCAGTAGCGGCGGGCGAGGGTGAGCAGGGTGCTGGAGGCGGGGGAGGCGTCGATGGCGATCAGGACGGGGCTGGGCATTGGAATCCTTGGCAGAGGCTGGCTTGTGTGGCTGCAGGATCGGCCCTTTCGCGGGCATGCCCGCTCCCACAGGTGTGTCGCACAACATTCGGGAATTGTGGGGGGGCCTGTGGCAGCGGGCGCGCCCGCGAAAAGGCCAGACCTGCCAATGCTTCATTTAAG  >  NC_002947/1450418‑1450705

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑