Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,463,318	C→T	5.0%	L288L (TTG→TTA)	algL ←	alginate lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,463,318	0	C	T	5.0%	240.5 / 2.2	79	L288L (TTG→TTA)	algL	alginate lyase
Reads supporting (aligned to +/- strand):  ref base C (37/38);  new base T (2/2);  total (39/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.67e-01

ACGCAGTGGTACAGCGCACACCAGGGCTCGAGCCAGGAGTACTTGCTGTCAACCTTGAGGTCGGTCATGTCCTGTTTCTTGCCCGCCCGCGCCTTGAAGTGGCTG‑‑‑GGGTCGCGGG‑‑CGCCGGC‑CAGCACGCCTTCACCCAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGCGC‑GCGCTGCTGGCGCTTGATTTCGTTGGGCAGGAAGCCCTGGTCAT  >  NC_002947/1463172‑1463463                                                                                                                                                         |                                                                                                                                                   aCGCAGTGGTACAGCGCACACCAGGGCTCGAGCCAGGAGTACTTGCTGTCAACCTTGAGGTCGGTCATGTCCTGTTTCTTGCCCGCCCGCGCCTTGAAGTGGCTG‑‑‑GGGTCGCGGG‑‑CGCCGGC‑CAGCACGCCTTCACCCAGGCGCTGCaa                                                                                                                                                  <  4:312373/149‑1 (MQ=255)         gTACAGCGCACACCAGGGCTCGAGCCAGGAGTACTTGCTGTCAACCTTGAGGTCGGTCATGTCCTGTTTCTTGCCCGCCCGCGCCTTGAAGTGGCTG‑‑‑GGGTCGCGGG‑‑CGCCGGC‑CAGCACGCCTTCACCCAGGCGCTGCAAGGCGAAgt                                                                                                                                          <  7:230311/149‑1 (MQ=255)            cAGCGCACACCAGGGCTCGAGCCAGGAGTACTTGCTGTCAACCTTGAGGTCGGTCATGTCCTGTTTCTTGCCCGCCCGCGCCTTGAAGTGGCTG‑‑‑GGGTCGCGGG‑‑CGCCGGC‑CAGCACGCCTTCACCCAGGCGCTGTAAGGCGa                                                                                                                                             <  4:273963/143‑1 (MQ=255)            cAGCGCACACCAGGGCTCGAGCCAGGAGTACTTGCTGTCAACCTTGAGGTCGGTCATGTCCTGTTTCTTGCCCGCCCGCGCCTTGAAGTGGCTG‑‑‑GGGTCGCGGG‑‑CGCCGGC‑CAGCACGCCTTCACCCAGGCGCTGTAAGGCGa                                                                                                                                             <  4:278822/143‑1 (MQ=255)            cAGCGCACACCAGGGCTCGAGCCAGGAGTACTTGCTGTCAACCTTGAGGTCGGTCATGTCCTGTTTCTTGCCCGCCCGCGCCTTGAAGTGGCTG‑‑‑GGGTCGCGGG‑‑CGCCGGC‑CAGCACGCCTTCACCCAGGCGCTGTAAGGCGa                                                                                                                                             >  3:278822/1‑143 (MQ=255)            cAGCGCACACCAGGGCTCGAGCCAGGAGTACTTGCTGTCAACCTTGAGGTCGGTCATGTCCTGTTTCTTGCCCGCCCGCGCCTTGAAGTGGCTG‑‑‑GGGTCGCGGG‑‑CGCCGGC‑CAGCACGCCTTCACCCAGGCGCTGTAAGGCGa                                                                                                                                             >  3:273963/1‑143 (MQ=255)              gcgcACACCAGGGCTCGAGCCAGGAGTACTTGCTGTCAACCTTGAGGTCGGTCATGTCCTGTTTCTTGCCCGCCCGCGCCTTGAAGTGGCTG‑‑‑GGGTCGCGGG‑‑CGCCGGC‑CAGCACGCCTTCACCCAGGCGCTGCAAGGCGAAGTTGTtc                                                                                                                                     >  3:295564/1‑149 (MQ=255)                    acCAGGGCTCGAGCCAGGAGTACTTGCTGTCAACCTTGAGGTCGGTCATGTCCTGTTTCTTGCCCGCCCGCGCCTTGAAGTGGCTG‑‑‑GGGTCGCGGG‑‑CGCCGGC‑CAGCACGCCTTCACCCAGGCGCTGCAAGGCGAAGTTGTTCTCGgcg                                                                                                                               >  4:35026/1‑149 (MQ=255)                    acCAGGGCTCGAGCCAGGAGTACTTGCTGTCAACCTTGAGGTAGGTCATGTCCTGTTTCTTGACCGCCCGCGCCTTGAAGTGGCTG‑‑‑GGGTCGCGGG‑‑CGCCGGC‑CAGCACGCCTTCACCCAGGCGCTGCAAGGCGAAGTGGTTCTCGgcg                                                                                                                               >  8:160274/1‑149 (MQ=255)                     ccAGGGCTCGAGCCAGGAGTACTTGCTGTCAACCTTGAGGTCGGTCATGTCCTGTTTCTTGCCCGCCCGCGCCTTGAAGTGGCTG‑‑‑GGGTCGCGGG‑‑CGCCGGC‑CAGCACGCCTTCACCCAGGCGCTGCAAGGCGAAGTTGTTCTCGgcgc                                                                                                                              <  3:227606/149‑1 (MQ=255)                       aGGGCTCGAGCCAGGAGTACTTGCTGTCAACCTTGAGGTCGGTCATGTCCTGTTTCTTGCCCGCCCGCGCCTTGAAGTGGCTG‑‑‑GGGTCGCGGG‑‑CGCCGGC‑CAGCACGCCTTCACCCAGGCGCTGCAAGGCGAAGTTGTTCTCGgcgcg                                                                                                                             >  4:71313/1‑148 (MQ=255)                       aGGGATCGAGCCAGGAGTACTTGCTGTCAACCTTGAGGTCGTTCATGTCCTGTTTCTTGCCCGCCCGCGCCTTGAAGTGGCTG‑‑‑GGGTCGCGGG‑‑CGCCGGC‑CAGCACGCCTTCACCCAGGCGCTGCAAGGCGAAGTTGTTCTCGgcgcgc                                                                                                                            <  6:101449/149‑1 (MQ=255)                         ggCTCGAGCCAGGAGTACTTGCTGTCAACCTTGAGGTCGGTCATGTCCTGTTTCTTGCCCGCCCGCGCCTTGAAGTGGCTG‑‑‑GGGTCGCGGG‑‑CGCCGGC‑CAGCACGCCTTCACCCAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAg                                                                                                                          <  6:326034/149‑1 (MQ=255)                          gCTCGAGCCAGGAGTACTTGCTGTCAACCTTGAGGTCGGTCATGTCCTGTTTCTTGCCCGCCCGCGCCTTGAAGTGGCTG‑‑‑GGGTCGCGGG‑‑CGCCGGC‑CAGCACGCCTTCACCCAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAgg                                                                                                                         <  7:91192/149‑1 (MQ=255)                           cTCAAGCCAGGAGTACTTGCTGTCAACCTTGAGGTCGGACATGTCCTGATTATTGCCCTCCCGCGCCTTGAATTGGCAG‑‑‑GGGTCGCGGT‑‑CGCCGGC‑CAGCACGCCTTCACCCAGGCGCTCCAAGCCGAAGTTGTTCTCGTCGCGCAGGt                                                                                                                        >  2:109670/1‑149 (MQ=255)                                gCCAGGAGTACTTGCTGTCAACCTTGAGGGCGGTCATGTCCTGTTTCTTGCCCGCCCGCGCCTTGAAGTGGCTG‑‑‑GGGTCGCGGG‑‑CGCCGGCACAGCACGCCTTCACCCAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGAc                                                                                                                    <  1:104405/149‑1 (MQ=255)                                 ccAGGAGTACTTGCTGTCAACCTTGAGGTCGGTCATGTCCTGTTTCTTGCCCGCCCGCGCCTCGAAGTGGAAGGGCGGGTCGCGGGCCCGGCGGC‑CACCACGCCTTCACCCAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTc                                                                                                                       >  1:118662/1‑149 (MQ=255)                                          cTTGCTGTCAACCTTGAGGTCGGTCATGTCCTGTTTCTTGCCCGCCCGCGCCTTGAAGTGGCTG‑‑‑GGGTCGCGGG‑‑CGCCGGC‑CAGCACGCCTTCACCCAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCt                                                                                                         >  7:335621/1‑149 (MQ=255)                                           ttGCTGTCAACCTTGAGGTCGGTCATGTCCTGTTTCTTGCCCGCCCGCGCCTTGAAGTGGCTG‑‑‑GGGTCGCGGG‑‑CGCCGGC‑CAGCACGCCTTCACCCAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTg                                                                                                        >  7:300544/1‑149 (MQ=255)                                           ttGCTGTCAACCTTGAGGTCGGTCATGTCCTGTTTCTTGCCCGCCCGCGCCTTGAAGTGGCTG‑‑‑GGGTCGCGGG‑‑CGCCGGC‑CAGCACGCCTTCACCCAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTg                                                                                                        >  4:27556/1‑149 (MQ=255)                                           ttGCTGTCAACCTTGAGGTCGGTCATGTCCTGTTTCTTGCCCGCCCGCGCCTTGAAGTGGCTG‑‑‑GGGTCGCGGG‑‑CGCCGGC‑CAGCACGCCTTCACCCAGGCGCTGCAAGGCGAAGATGTTCTCGGCGCGCAGGTCGACGCCATTGGCGTg                                                                                                        >  4:262072/1‑149 (MQ=255)                                             gCTGTCAACCTTGAGGTCGGTCATGTCCTGTTTCTTGCCCGCCCGCGCCTTGAAGTGGCTG‑‑‑GGGTCGCGGG‑‑CGCCGGC‑CAGCACGCCTTCACCCAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTgg                                                                                                       <  8:193211/148‑1 (MQ=255)                                             gCTGTCAACCTTGAGGTCGGTCATGTCCTGTTTCTTGCCCGCCCGCGCCTTGAAGTGGCTG‑‑‑GGGTCGCGGG‑‑CGCCGGC‑CAGCACGCCTTCACCCAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTg                                                                                                        <  4:295564/147‑1 (MQ=255)                                              cTGTCAACCTTGAGGTCGGTCATGTCCTGTTTCTTGCCCGCCCGCGCCTTGAAGTGGCTG‑‑‑GGGTCGCGGG‑‑CGCCGGC‑CAGCACGCCTTCACCCAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTggg                                                                                                      <  2:157261/148‑1 (MQ=255)                                               tGTCAACCTTGAGGTCGGTCATGTCCTGTTTCTTGCCCGCCCGCGCCTTGAAGTGGCTG‑‑‑GGGTCGCGGG‑‑CGCCGGC‑CAGCACGCCTTCACCCAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGc                                                                                                     >  6:254240/1‑148 (MQ=255)                                                 tCAACCTTGAGGTCGGTCATGTCCTGTTTCTTGCCCGCCCGCGCCTTGAAGTGGCTG‑‑‑GGGTCGCGGG‑‑CGCCGGC‑CAGCACGCCTTCACCCAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCg                                                                                                    >  7:337288/1‑147 (MQ=255)                                                   aaCCTTGAGGTCGGTCATGTCCTGTTTCTTGCCCGCCCGCGCCTTGAAGTGGCTG‑‑‑GGGTCGCGGG‑‑CGCCGGC‑CAGCACGCCTTCACCCAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGc                                                                                                >  2:164748/1‑149 (MQ=255)                                                   aaCCTTGAGGTCGGTCATGTCCTGTTTCTTGCCCGCCCGCGCCTTGAAGTGGCTG‑‑‑GGGTCGCGGG‑‑CGCCGGC‑CAGCACGCCTTCACCCAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGc                                                                                                <  7:164493/149‑1 (MQ=255)                                                    aCCTTGAGGTCGGTCATGTCCTGTTTCTTGCCCGCCCGCGCCTTGAAGTGGCTG‑‑‑GGGTCGCGGG‑‑CGCCGGC‑CAGCACGCCTTCACCCAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCCCAGGTCGACACCATTGGCCTGGGCGAAGCt                                                                                               <  1:31176/149‑1 (MQ=255)                                                      cgtcaGGGCGGTCATGTCCTGTGTCTTGCCCCCCCGCGCCTTGAAGGGGCTG‑‑‑GGGTCGCGGG‑‑CGCCGGC‑CAGCACGCCTTCACCCAGGCGCGGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTgg                                                                                             <  8:271763/145‑1 (MQ=255)                                                      cTTGAGGTCGGTCATGTCCTGTTTCTTGCCCGCCCGCGCCTTGAAGTGGCTG‑‑‑GGGTCGCGGG‑‑CGCCGGC‑CAGCACGCCTTCACCCAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTgg                                                                                             >  4:143742/1‑149 (MQ=255)                                                      cTTGAGGTCGGTCATGTCCTGTTTCTTGCCCGCCCGCGCCTTGAAGTGGCTG‑‑‑GGGTCGCGGG‑‑CGCCGGC‑CAGCACGCCTTCACCCAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTgg                                                                                             <  7:26098/149‑1 (MQ=255)                                                                gtcATGTCCTGTTGCTTGCCCGCCCGCGCCTTGAAGTGGCTG‑‑‑GGGTCGCGGG‑‑CGCCGGC‑CAGCACGCCTTCACCCAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGc                                                                                   <  1:109670/149‑1 (MQ=255)                                                                   aTGTCCTGTTTCTTGCCCGCCCGCGCCTTGAAGTGGCTG‑‑‑GGGTCGCGGG‑‑CGCCGGC‑CAGCACGCCTTCACCCAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCa                                                                                 <  8:300544/148‑1 (MQ=255)                                                                    tGTCCTGTTTCTTGCCCGCCCGCGCCTTGAAGTGGCTG‑‑‑GGGTCGCGGG‑‑CGCCGGC‑CAGCACGCCTTCACCCAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAgc                                                                               >  7:38520/1‑149 (MQ=255)                                                                      tCCTGTTTCTTGCCCGCCCGCGCCTTGAAGTGGCTG‑‑‑GGGTCGCGGG‑‑CGCCGGC‑CAGCACGCCTTCACCCAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAgcgg                                                                             >  4:45367/1‑149 (MQ=255)                                                                         tGTTTCTTGCCCGCCCGCGCCTTGAAGTGGCTG‑‑‑GGGTCGCGGG‑‑CGCCGGC‑CAGCACGCCTTCACCCAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGcggcgg                                                                          >  7:187246/1‑149 (MQ=255)                                                                             tCTTGCCCGCCCGCGCCTTGAAGTGGCTG‑‑‑GGGTCGCGGG‑‑CGCCGGC‑CAGCACGCCTTCACCCAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCaa                                                                       <  1:314373/148‑1 (MQ=255)                                                                               ttGCCCGCCCGCGCCTTGAAGTGGCTG‑‑‑GGGTCGCGGG‑‑CGCCGGC‑CAGCACGCCTTCACCCAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGc                                                                    >  6:284027/1‑149 (MQ=255)                                                                                 gcccgcccGCGCCTTGAAGTGGCTG‑‑‑GGGTCGCGGG‑‑CGCCGGC‑CAGCACGCCTTCACCCAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGc                                                                    <  3:27556/147‑1 (MQ=255)                                                                                   ccgcccgcGCCTTGAAGTGGCTG‑‑‑GGGTCGCGGG‑‑CGCCGGC‑CAGCACGCCTTCACCCAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAg                                                                >  1:91123/1‑149 (MQ=255)                                                                                   ccgcccgcGCCTTGAAGTGGCTG‑‑‑GGGTCGCGGG‑‑CGCCGGC‑CAGCACGCCTTCACCCAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAg                                                                <  5:254240/149‑1 (MQ=255)                                                                                      cccgcGCCTTGAAGTGGCTG‑‑‑GGGTCGCGGG‑‑CGCCGGC‑CAGCACGCCTTCACCCAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTtg                                                             <  3:35026/149‑1 (MQ=255)                                                                                         gcgcCTTGAAGTGGCTG‑‑‑GGGGCGCGGG‑‑CGCCGGC‑CAGCACGCCTTCACCCAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGa                                                          <  1:153199/149‑1 (MQ=255)                                                                                            ccTTGAAGTGGCTG‑‑‑GGGTCGCGGG‑‑CGCCGGC‑CAGCACGCCTTCACCCAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAg                                                       <  8:335621/149‑1 (MQ=255)                                                                                            ccTTGAAGTGGCTG‑‑‑GGGTCGCGGG‑‑CGCCGGC‑CAGCACGCCTTCACCCAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAg                                                       <  5:284027/149‑1 (MQ=255)                                                                                                      gCTG‑‑‑GGGTCGCGGG‑‑CGCCGGC‑CAGCACGCCTTCACCCAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGTCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAgcgc‑gc                                            >  6:45068/1‑149 (MQ=255)                                                                                                      gCTG‑‑‑GGGTCGCGGG‑‑CGCCGGC‑CAGCACGCCTTCACCCAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAgcgc‑gc                                            <  6:75207/149‑1 (MQ=255)                                                                                                        tG‑‑‑GGGTCGCGGG‑‑CGCCGGC‑CAGCACGCCTTCACCCAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAgcgc‑gcgc                                          >  7:78953/1‑149 (MQ=255)                                                                                                             gggTCGCGGG‑‑CGCCGGC‑CAGCACGCCTTCACCCAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGCGC‑GCgctg                                        >  5:6925/1‑149 (MQ=255)                                                                                                                tCGCGGG‑‑CGCCGGC‑CAGCACGCCTTCACCCAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGCGC‑GCGctgctg                                     >  6:18803/1‑149 (MQ=255)                                                                                                                    ggg‑‑CGCCGGC‑CAGCACGCCTTCACCCAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGCGC‑GCGCTGCTGgcgc                                 >  3:333657/1‑149 (MQ=255)                                                                                                                      g‑‑cgcCGGC‑CAGCACGCCTTCACCCAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGCGC‑GCGCTGCTGGCGCtt                               >  8:331504/1‑149 (MQ=255)                                                                                                                            cGGC‑CAGCACGCCTTCACCCAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGTCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGCGC‑GCGCTGCTGGCGCTTGAtt                           <  5:45068/149‑1 (MQ=255)                                                                                                                            cGGC‑CAGCACGCCTTCACCCAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGCGC‑GCGCTGCTGGCGCTTGAtt                           >  6:299268/1‑149 (MQ=255)                                                                                                                              gC‑CAGCACGCCTTCACCCAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGCGC‑GCGCTGCTGGCGCTTGATTTc                         >  3:279273/1‑149 (MQ=255)                                                                                                                                 cAGCACGCCTTCACCCAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGCGC‑GCGCTGCTGGCGCTCGATTTCGt                       >  6:151999/1‑149 (MQ=255)                                                                                                                                  aGCACGCCTTCACCCAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGCGC‑GCGCTGCTGGCGCTTGATTTCGtt                      <  8:78953/149‑1 (MQ=255)                                                                                                                                  aGCACGCCTTCACCCAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGCGC‑GCGCTGCTGGCGCTTGATTTCGtt                      <  5:18803/149‑1 (MQ=255)                                                                                                                                  aGCACGCCTTCACCCAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGCGC‑GCGCTGCTGGCGCTCGATTTCGtt                      <  5:151999/149‑1 (MQ=255)                                                                                                                                   tcACGCCTTCACCCAGGCGCTTCAATGCTTATTTTTTCTCGGCGCGCATGTCGAAGCCATTTGTCTGGGTGAATCTGTCAATCATTGTCAGCGGCGGCAATGCGTAGTTGTTATATGCCAGCGCTTCGCTGCTGGCGCTTGATTTCGtt                      <  7:56507/148‑1 (MQ=255)                                                                                                                                      cGCCTTCACCCAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGCGC‑GCGCTGCTGGCGCTTGATTTCGTTGGGc                  <  3:143742/149‑1 (MQ=255)                                                                                                                                         cTTCACCCAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGCGC‑GCGCTGCTGGCGCTTGATTTCGTTGGGCAg                >  5:106872/1‑148 (MQ=255)                                                                                                                                             aCCCAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGCGC‑GCGCTGCTGGCGCTTGATTTCGt                       >  4:272508/1‑137 (MQ=255)                                                                                                                                             aCCCAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGCGC‑GCGCTGCTGGCGCTTGATTTCGt                       <  3:272508/137‑1 (MQ=255)                                                                                                                                               ccAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAg                                                                      >  4:46555/1‑89 (MQ=255)                                                                                                                                               ccAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGtt                                                              >  4:367260/1‑97 (MQ=255)                                                                                                                                               ccAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGtt                                                              <  3:367260/97‑1 (MQ=255)                                                                                                                                               ccAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGCGC‑GCGCTGCTGGCGCTTGATTTCGTTGGGCAGGAAGccc         <  8:187246/149‑1 (MQ=255)                                                                                                                                                cAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGCGC‑GCGCTGCTGGCGCTTGATTTCGTTGGGCAGGAAGCCCt        >  6:89093/1‑149 (MQ=255)                                                                                                                                                  ggCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGCGC‑GCGCTGCTGGCGCTTGATTTCGTTGGGCAGGAAGCCCTgg      <  2:118662/149‑1 (MQ=255)                                                                                                                                                   gcgcTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGCGC‑GCGCTGCTGGCGCTTGATTTCGTTGGGCAGGAAGCCCTGGt     >  8:123445/1‑149 (MQ=255)                                                                                                                                                     gcTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGCGC‑GCGCTGCTGGCGCTTGATTTCGTTGGGCAGGAAGcc          <  7:59416/142‑1 (MQ=255)                                                                                                                                                     gcTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGCGC‑GCGCTGCTGGCGCTTGATTTCGTTGGGCAGGAAGcc          >  8:59416/1‑142 (MQ=255)                                                                                                                                                     gcTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGCGC‑GCGCTGCTGGCGCTTGATTTCGTTGGGCAGGAAGCCCTGGTc    >  8:84768/1‑148 (MQ=255)                                                                                                                                                      cTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGCGC‑GCGCTGCTGGCGCTTGATTTCGTTGGGCAGGAAGCCCTGGTCAt  <  5:62141/149‑1 (MQ=255)                                                                                                                                                      cTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGCGC‑GCGCTGCTGGCGCTTGATTTCGTTGGGCAGGAAGCCCTGGTCAt  <  7:91318/149‑1 (MQ=255)                                                                                                                                                        gCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTgg                                                                                             >  8:214674/1‑57 (MQ=255)                                                                                                                                                        gCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTgg                                                                                             <  7:214674/57‑1 (MQ=255)                                                                                                                                                         |                                                                                                                                                   ACGCAGTGGTACAGCGCACACCAGGGCTCGAGCCAGGAGTACTTGCTGTCAACCTTGAGGTCGGTCATGTCCTGTTTCTTGCCCGCCCGCGCCTTGAAGTGGCTG‑‑‑GGGTCGCGGG‑‑CGCCGGC‑CAGCACGCCTTCACCCAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGCGC‑GCGCTGCTGGCGCTTGATTTCGTTGGGCAGGAAGCCCTGGTCAT  >  NC_002947/1463172‑1463463

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑