Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,472,713	C→G	5.2%	S38S (TCG→TCC) ‡	alg8 ←	glycosyltransferase Alg8

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,472,713	0	C	G	5.2%	253.5 / 2.2	77	S38S (TCG→TCC) ‡	alg8	glycosyltransferase Alg8
Reads supporting (aligned to +/- strand):  ref base C (37/36);  new base G (2/2);  total (39/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.93e-01

GTGGCATTACCCATTTTCTGCACCTTGCGGCGCAGGTACGGGTACACGCCGTAGAGGAAGATCATGCCGCGGATGAAATGGGTGGCGCCCATGGAGTAGCGCCAGATGCCGACTGCGCCGACCAGGAAGATGAAGTGCTTCGACTGCGAGTCGAAGATATCAGCTGGCAGGGCCAGGGCGATCAGCATGAGCAGGCTCATGTAGAGCAGCCAC‑CCGGCGCACTGCAACAGCACTGTCTGGAGCCTTTGCATGTTCAGCATCCGTCGAGAATTCGGGAAAGGGTGGGCA  >  NC_002947/1472567‑1472854                                                                                                                                                   |                                                                                                                                               gTGGCATTACCCATTTTCTGCACCTTGCGGCGCAGGTACGGGTACACGCCGTAGAGGAAGATCATGCCGCGGATGAAATGGGTGGCGCCCATGGAGTAGCGCCAGATGCCGACTGCGCCGACCAGGAAGATGAAGTGCTTCGACTGCGa                                                                                                                                              <  6:751/149‑1 (MQ=255)    gCATTACCCATTTTCTGCACCTTGCGGCGCAGGTACGGGTACACGCCGTAGAGGAAGATCATGCCGCGGATGAAATGGGTGGCGCCCATGGAGTAGCGCCAGATGCCGACTGCGCCGACCAGGAAGATGAAGTGCTTCGACTGCGAGTc                                                                                                                                           >  2:25377/1‑149 (MQ=255)       ttACCCATTTTCTGCACCTTGCGGCGCAGGTACGGGTACACGCCGTAGAGGAAGATCATGCCGCGGATGAAATGGGTGGCGCCCATGGAGTAGCGCCAGATGCCGACTGCGCCGACCAGGAAGATGAAGTGCTTCGACTGCGAGTCg                                                                                                                                          >  8:1678/1‑147 (MQ=255)         aCCCATTTTCTGCACCTTGCGGCGCAGGTACGGGTACACGCCGTAGAGGAAGATCATGCCGCGGATGAAATGGGTGGCGCCCATGGAGTAGCGCCAGATGCCGACTGCGCCGACCAGGAAGATGAAGTGCTTCGACTGCGAGTCg                                                                                                                                          >  8:31391/1‑145 (MQ=255)         aCCCATTTTCTGCACCTTGCGGCGCAGGTACGGGTACACGCCGTAGAGGAAGATCATGCCGCGGATGAAATGGGTGGCGCCCATGGAGTAGCGCCAGATGCCGACTGCGCCGACCAGGAAGATGAAGTGCTTCGACTCGCAGTCGAAg                                                                                                                                       <  1:324273/148‑1 (MQ=255)           ccATTTTCTGCACCTTGCGGCGCAGGTACGGGTACACGCCGTAGAGGAAGATCATGCCGCGGATGAAATGGGTGGCGCCCATGGAGTAGCGCCAGATGCCGACTGCGCCGACCAGGAAGATGAAGTGCTTCGACTGCGAGTCGAAGata                                                                                                                                    >  3:340228/1‑149 (MQ=255)            cATTTTCTGCACCTTGCGGCGCAGGTACGGGTACACGCCGTAGAGGAAGATCATGCCGCGGATGAAATGGGTGGCGCCCATGGAGTAGCGCCAGATGCCGACTGCGCCGACCAGGAAGATGAAGTGCTTCGACTGCGAGTCGAAGatat                                                                                                                                   >  5:56056/1‑149 (MQ=255)             aTTTTCTGCACCTTGCGGCGCAGGTACGGGTACACGCCGTAGAGGAAGATCATGCCGCGGATGAAATGGGTGGCGCCCATGGAGTAGCGCCAGATGCCGACTGCGCCGACCAGGAAGATGAAGTGCTTCGACTGCGAGTCGAAGatat                                                                                                                                   >  7:104056/1‑148 (MQ=255)              ttttCTGCACCTTGCGGCGCAGGTACGGGTACACGCCGTAGAGGAAGATCATGCCGCGGATGAAATGGGTGGCGCCCATGGAGTAGCGCCAGATGCCGACTGCGCCGACCAGGAAGATGAAGTGCTTCGACTGCGAGTc                                                                                                                                           <  7:318710/139‑1 (MQ=255)              ttttCTGCACCTTGCGGCGCAGGTACGGGTACACGCCGTAGAGGAAGATCATGCCGCGGATGAAATGGGTGGCGCCCATGGAGTAGCGCCAGATGCCGACTGCGCCGACCAGGAAGATGAAGTGCTTCGACTGCGAGTc                                                                                                                                           >  8:318710/1‑139 (MQ=255)              ttttCTGCACCTTGCGGCGCAGGTACGGGTACACGCCGTAGAGGAAGATCATGCCGCGGATGAAATGGGTGGCGCCCATGGAGTAGCGCCAGATGCCGACTGCGCCGACCAGGAAGATGAAGTGCTTCGACTGCGAGTCGAAGATATCa                                                                                                                                 <  4:321136/149‑1 (MQ=255)                     cACCTTGCGGCGCAGGTACGGGTACACGCCGTAGAGGAAGATCATGCCGCGGATGAAATGGGTGGCGCCCATGGAGTAGCGCCAGATGCCGACTGCGCCGACCAGGAAGATGAAGTGCTTCGACTGCGAGTCGAAGATATCAGCTg                                                                                                                             <  1:342945/146‑1 (MQ=255)                      aCCTTGCGGCGCAGGTACGGGTACACGCCGTAGAGGAAGATCATGCCGCGGATGAAATGGGTGGCGCCCATGGAGTAGCGCCAGATGCCGACTGCGCCGACCAGGAAGATGAAGTGCTTCGACTGCGAGTCGAAGATATCAGCTGGcag                                                                                                                         >  8:274878/1‑149 (MQ=255)                        cTTGCGGCGCAGGTACGGGTACACGCCGTAGAGGGAGATCATGCCGCGGATGAAATGGGTGGCGCCCATGGAGTAGCGCCAGATGCCGACTGCGCCGACCAGGAAGATGAAGTGCTTCGACTGCGAGTCGAAGATATCAGCTGGcaggg                                                                                                                       >  3:174634/1‑149 (MQ=255)                         ttGCGGCGCAGGTACGGGTACACGCCGTAGAGGAAGATCATGCCGCGGATGAAATGGGTGGCGCCCATGGAGTAGCGCCAGATGCCGACTGCGCCGACCAGGAAGATGAAGTGCTTCGACTGCGAGTCGAAGATATCAGCTGGcagggc                                                                                                                      >  4:196226/1‑149 (MQ=255)                           gcggcgCAGGTACGGGTACACGCCGTAGAGGAAGATCATGCCGCGGATGAAATGGGTGGCGCCCATGGAGTAGCGCCAGATGCCGACTGCGCCGACCAGGAAGATGAAGTGCTTCGACTGCGAGTCGAAGATATCAGCTGGcagggc                                                                                                                      <  3:196226/147‑1 (MQ=255)                            cggcgCAGGTACGGGTACACGCCGTAGAGGAAGATCATGCCGCGGATGAAATGGGTGGCGCCCATGGAGTAGCGCCAGATGCCGACTGCGCCGACCAGGAAGATGAAGTGCTTCGACTGCGAGTCGAAGATATCAGCTGGcagggccag                                                                                                                   <  1:25377/149‑1 (MQ=255)                            cggcgCAGGTACGGGTACACGCCGTAGAGGAAGATCATGCCGCGGATGAAATGGGTGGCGCCCATGGAGTAGCGCCAGATGCCGACTGCGCCGACCAGGAAGATGAAGTGCTTCGACTGCGAGTCGAAGATATCAGCTGGcagggcca                                                                                                                    <  3:38366/148‑1 (MQ=255)                              gcgcAGGTACGGGTACACGCCGTAGAGGAAGATCATGCCGCGGATGAAATGGGTGGCGCCCATGGAGTAGCGCCAGATGCCGACTGCGCCGACCAGGAAGATGAAGTGCTTCGACTGCGAGTCGAAGATATCAGCTGGcagggccaggg                                                                                                                 <  5:111062/149‑1 (MQ=255)                              gcgcAGGTACGGGTACACGCCGTAGAGGAAGATCATGCCGCGGATGAAATGGGTGGCGCCCAT‑GAGTAGCGCCAGATGCCGACTGCGCCGACCAGGAAGATGAAGTGCTTCGACTGCGAGTCAAAGATATCAGCTGGcagggccagggc                                                                                                                <  2:351925/149‑1 (MQ=255)                                 cAGGTACGGGTAAACGCCGTAGAGGATGATCATGGCGCGTTTGAAATGTGTGGCGCCCATGGAGTAGCGCCTGATGCCGAGTGCGCGGACCAGGAAGATTAAGTGCTTCGACTGCGAGTCGAAGat                                                                                                                                     <  1:376960/126‑1 (MQ=255)                                 cAGGTACGGCTACACGCCGTAGAGGAAGATCATGCCGCGGATGAAATGGGTGGCGCCCATGGAGTAGCGCCAGATGCCGACTGCGCCCACCAGGAAGATGAAGTGCTTCGACTGCGAGTCGAAGat                                                                                                                                     >  2:376960/1‑126 (MQ=255)                                       cGGGTACACGCCGTAGAGGAAGATCATGCCGCGGATGAAATGGGTGGCGCCCATGGAGTAGCGCCAGATGCCGACTGCGCCGACCAGGAAGATGAAGTGCTTCGACTGCGAGTCGAAGATATCAGCTGGCAGGGCCAGGGCGATCAGCa                                                                                                        <  3:108788/149‑1 (MQ=255)                                          gTACACGCCGTAGAGGAAGATCATGCCGCGGATGAAATGGGTGGCGCCCATGGAGTAGCGCCAGATGCCGACTGCGCCGACCAGGAAGATGAAGTGCTTCGACTGCGAGTCGAAGATATCAGCTGGcag                                                                                                                         <  3:58441/129‑1 (MQ=255)                                          gTACACGCCGTAGAGGAAGATCATGCCGCGGATGAAATGGCTGGCGCCCATGGAGTAGCGCCAGATGCCGACTGCGCCGACCAGGAAGATGAAGTGCTTCGACTGCGAGTCGAAGATATCAGCTGGcag                                                                                                                         >  4:58441/1‑129 (MQ=255)                                           tACACGCCGTAGAGGAAGATCATGCCGCGGATGAAATGGGTGGCGCCCATGGAGTAGCGCCAGATGCCGACTGCGCCGACCAGGAAGATGAAGTGCTTCGACTGCGAGTCGAAGATATCAGCTGGCAGGGCCAGGGCGATCAGCATGAg                                                                                                    >  4:196455/1‑149 (MQ=255)                                             cacGCCGTAGAGGAAGATCATGCCGCGGATGAAATGGGTGGCGCCCATGGAGTAGCGCCAGATGCCGACTGCGCCGACCAGGAAGATGAAGTGCTTCGACTGCGAGTCGAAGATATCAGCTGGCAGGGCCAGGGCGATCAGCATGAg                                                                                                    >  6:187513/1‑147 (MQ=255)                                              acGCCGTAGAGGAAGATCATGCCGCGGATGAAATGGGTGGCGCCCATGGAGTAGCGCCAGATGCCGACTGCGCCGACCAGGAAGATGAAGTGCTTCGACTGCGAGTCGAAGATATCAGCTGGCAGGGCCAGGGCGATCAGCATGAGCAg                                                                                                 >  5:14104/1‑149 (MQ=255)                                               cGCCGTAGAGGAAGATCATGCCGCGGATGAAATGGGTGGCGCCCATGGAGTAGCGCCAGATGCCGACTGCGCCGACCAGGAAGATGAAGTGCTTCGACTGCGAGTCGAAGATATCAGCTGGCAGGGCCAGGGCGATCAGCATGAGCAgg                                                                                                >  8:573/1‑149 (MQ=255)                                                  cGTAGAGGAAGATCATGCCGCGGATGAAATGGGTGGCGCCCATGGAGTAGCGCCAGATGCCGACTGCGCCGACCAGGAAGATGAAGTGCTTCGACTGCGAGTCGAAGATATc                                                                                                                                  >  6:329931/1‑112 (MQ=255)                                                  cGTAGAGGAAGATCATGCCGCGGATGAAATGGGTGGCGCCCATGGAGTAGCGCCAGATGCCGACTGCGCCGACCAGGAAGATGAAGTGCTTCGACTGCGAGTCGAAGATATc                                                                                                                                  <  5:329931/112‑1 (MQ=255)                                                    tAGAGGAAGATCATGCCGCGGATGAAATGGGTGGCGCCCATGGAGTAGCGCCAGATGCCGACTGCGCCGACCAGGAAGATGAAGTGCTTCGACTGCGAGTCGAAGATATCAGCTGGCAGGGCCAGGGCGATCAGCATGAGCAGGCTCAt                                                                                           <  7:274878/149‑1 (MQ=255)                                                     agagGAAGATGATGCCGCGGATGAAATGGGTGGCGCCCATGGAGTAGCGCCAGATGCCGACTGCGCCGACCAGGAAGATGAAGTGCTTCGACTGCGAGTCGAAGATATCAGCTGGCAGGGCCAGGGCGATCAGCATGAGCAGGCTCATg                                                                                          >  2:121942/1‑149 (MQ=255)                                                        ggAAGATCATGCCGCGGATGAAATGGGTGGCGCCCATGGAGTAGCGCCAGATGCCGACTGCGCCGACCAGGAAGATGAAGTGCTTCGACTGCGAGTCGAAGATATCAGCTGGCAGGGCCAGGGCGATCAGCATGAGCAGGCTCATGTa                                                                                        <  7:31391/148‑1 (MQ=255)                                                         gAAGATCATGCCGCGGATGAAATGGGTGGCGCCCATGGAGTAGCGCCAGATGCCGACTGCGCCGACCAGGAAGATGAAGTGCTTCGACTGCGAGTCGAAGATATCAGCTGGCAGGGCCAGGGCGATCAGCATGAGCAGGCTCATGTaga                                                                                      <  1:180850/149‑1 (MQ=255)                                                           aGATCATGCCGCGGATGAAATGGGTGGCGCCCATGGAGTAGCGCCAGATGCCGACTGCGCCGACCAGGAAGATGAAGTGCTTCGACTGCGAGTCGAAGATATCAGCTGGCAGGGCCAGGGCGATCAGCATGAGCAGGCTCATGTagag                                                                                     >  4:304773/1‑148 (MQ=255)                                                                aTGCCGCGGATGAAATGGGTGGCGCCCATGGAGTAGCGCCAGATGCCGACTCCGCCGACCAGGAAGATGAAGTGCTTCGACTGCGAGTCGAAGATATCAGCTGGCAGGGCCAGGGCGATCAGCATGAGCAGGCTCATGTAGAGCAGCCa                                                                               <  4:68573/149‑1 (MQ=255)                                                                    cgcgGATGAAATGGGTGGCGCCCATGGAGTAGCGCCAGATGCCGACTGCGCCGACCAGGAAGATGAAGTGGTTCGACTGCGAGTCGAAGATATCAGCTGGCAGGGCCAGGGCGATCAGCATGAGCAGGCTCATGTAGAGCAGCCAC‑CCg                                                                          <  8:281258/149‑1 (MQ=255)                                                                          tGAAATGGGTGGCGCCCATGGAGTAGCGCCAGATGCCGACTGCGCCGACCAGGAAGATGAAGTGCTTCGACTGCGAGTCGAAGATATCAGCTGGCAGGGCCAGGGCGATCAGCATGAGCAGGCTCATGTAGAGCAGCCAC‑CCGGCGCAc                                                                    >  6:56795/1‑149 (MQ=255)                                                                            aaaTGGGTGGCGCCCATGGAGTAGCGCCAGATGCCGACTGCGCCGACCAGGAAGATGAAGTGCTTCGACTGCGAGTCGAAGATATCAGCTGGCAGGGCCAGGGCGATCAGCATGAGCAGGCTCATGTAGAGCAGCCAC‑CCGGCGCACt                                                                   <  6:14104/148‑1 (MQ=255)                                                                              aTGGGTGGCGCCCATGGAGTAGCGCCAGATGCCGACTGCGCCGACCAGGAAGATGAAGTGCCTCGACTGCGAGTCGAAGATATCAGCTGGCAGGGCCAGGGCGATCAGCATGAGCAGGCTCATGTAGAGCAGCCAC‑CCGGCGCACTGc                                                                 >  5:335596/1‑148 (MQ=255)                                                                                          cATGGAGTAGCGCCAGATGCCGACTGCGCCGACCAGGAAGATGAAGTGCTTCGACTGCGAGTCGAAGATATCAGCTGGCAGGGCCAGGGCGATCAGCATGAGCAGGCTCATGTAGAGCAGCCAC‑CCGGCGCACTGCAACAGCACTGTCt                                                    <  8:88997/149‑1 (MQ=255)                                                                                           aTGGAGTAGCGCCAGATGCCGACTGCGCCGACCAGGAAGATGAAGTGCTTCGACTGCGAGTCGAAGATATCAGCTGGCAGGGCCAGGGCGATCAGCATGAGCAGGCTCATGTAGAGCAGCCAC‑CCGGCGCACTGCAACAGCACTGTCTg                                                   <  1:184556/149‑1 (MQ=255)                                                                                                 tAGCGCCAGATGCCGACTGCGCCGACCAGGAAGATGAAGTGCTTCGACTGCGAGTCGAAGATATCAGCTGGCAGGGCCAGGGCGATCAGCATGAGCAGGCTCATGTAGAGCAGCCAC‑CCGGCGCACTGCAACAGCACTGTCTGGAGCCt                                             <  5:56795/149‑1 (MQ=255)                                                                                                   gcgcCAGATGCCGACTGCGCCGACCAGGAAGATGAAGTGCTTCGACTGCGAGTCGAAGATATCAGCTGGCAGGGCCAGGGCGATCAGCATGAGCAGGCTCATGTAGAGCAGCCAC‑CCGGCGCACTGCAACAGCACTGTCTGGa                                                 >  1:334708/1‑143 (MQ=255)                                                                                                   gcgcCAGATGCCGACTGCGCCGACCAGGAAGATGAAGTGCTTCGACTGCGAGTCGAAGATATCAGCTGGCAGGGCCAGGGCGATCAGCATGAGCAGGCTCATGTAGAGCAGCCAC‑CCGGCGCACTGCAACAGCACTGTCTGGa                                                 <  2:334708/143‑1 (MQ=255)                                                                                                   gcgcCAGATGCCGACTGCGCCGACCAGGAAGATGAAGTGCTTCGACTGCGAGTCGAAGATATCAGCTGGCAGGGCCAGGGCGATCAGCATGAGCAGGCTCATGTAGAGCAGCCAC‑CCGGCGCACTGCAACAGCACTGTCTGGAGCCttt                                           <  4:174634/149‑1 (MQ=255)                                                                                                         gATGCCGACTGCGCCGACCAGGAAGATGAAGTGCTTCGACTGCGAGTCGAAGATATCAGCTGGCAGGGCCAGGGCGATCAGCATGAGCAGGCTCATGTAGAGCAGCCAC‑CCGGCGCACTGCAACAGCACTGTCTGGAGCCTTTGCATGt                                     <  7:1678/149‑1 (MQ=255)                                                                                                          aTGCCGACTGCGCCGACCAGGAAGATGAAGTGCTTCGACTGCGAGTCGAAGATATCAGCTGGCAGGGCCAGGGCGATCAGCATGAGCAGGCTCATGTAGAGCAGCCAC‑CCGGCGCACTGCAACAGCACTGTCTGGAGCCTTTGCATGtt                                    <  5:187513/149‑1 (MQ=255)                                                                                                             ccGACTGCGCCGACCAGGAAGATGAAGTGCTTCGACTGCGAGTCGAAGATATCAGCTGGCAGGGCCAGGGCGATCAGCATGAGCAGGCTCATGTAGAGCAGCCAC‑CCGGCGCACTGCAACAGCACTGTCTGGAGCCTTTGGATGTTCa                                  <  4:127840/148‑1 (MQ=255)                                                                                                               gACTGCGCCGACCAGGAAGATGAAGTGCTTCGACTGCGAGTCGAAGATATCAGCTGGCAGGGCCAGGGCGATCAGCATGAGCAGGCTCATGTAGAGCAGCCACTCCGGCGCACTGCAACAGCACTGTCTGGAGCCTTTGc                                         <  1:252657/140‑1 (MQ=255)                                                                                                               gACTGCGCCGACCAGGAAGATGAAGTGCTTCGACTGCGAGTCGAAGATATCAGCTGGCAGGGCCAGGGCGATCAGCATGAGCAGGCTCATGTAGAGCAGCCAC‑CCGGCGCACTGCAACAGCACTGTCTGGAGCCTTTGc                                         >  2:252657/1‑139 (MQ=255)                                                                                                                     gcCGACCAGGAAGATGAAGTGCTTCGACTGCGAGTCGAAGATATCAGCTGGCAGGGCCAGGGCGATCAGCATGAGCAGGCTCATGTAGAGCAGCCAC‑CCGGCGCACTGCAACAGCACTGTCTGGAGCCTTTGCATGTTCAGCATCCGTc                         >  7:46268/1‑149 (MQ=255)                                                                                                                     gcCGACCAGGAAGATGAAGTGCTTCGACTGCGAGACGAAGATATCAGCTGGCAGGGCCAGGGCGATCAGCATGAGCAGGCTCATGTAGAGCAGCCAA‑CCGGCGCACTGCAACAGCACTGTCTGGAGCCTTTGCATGTTCAGCATCCGTc                         >  1:276644/1‑149 (MQ=255)                                                                                                                       cGACCAGGAAGATGAAGTGCTTCGACTGCGAGTCGAAGATATCAGCTGGCAGGGCCAGGGCGATCAGCATGAGCAGGCTCATGTAGAGCAGCCAC‑CCGGCGCACTGCAACAGCACTGTCTGGAGCCTTTGCATGTTCAGCATCCGTc                         >  8:109670/1‑147 (MQ=255)                                                                                                                       cGACCAGGAAGATGAAGTGCTTCGACTGCGAGTCGAAGATATCAGCTGGCAGGGCCAGGGCGATCAGCATGAGCAGGCTCATGTAGAGCAGCCAC‑CCGGCGCACTGCAACAGCACTGTCTGGAGCCTTTGCATGTTCAGCATCCGTCga                       <  3:196455/149‑1 (MQ=255)                                                                                                                             ggAAGATGAAGTGCTTCGACTGCGAGTCGAAGATATCAGCTGGCAGGGCCAGGGCGATCAGCATGAGCAGGCTCATGTAGAGCAGCCAC‑CCGGCGCACTGCAACAGCACTGTCTGGAGCCTTTGCATGTTCAGCATCCGTCGAGAATTc                 >  3:297514/1‑149 (MQ=255)                                                                                                                              gAAGATGAAGTGCTTCGACTGCGAGTCGAAGATATCAGCTGGCAGGGCCAGGGCGATCAGCATGAGCAGGCTCATGTAGAGCAGCCAC‑CCGGCGCACTGCAACAGCACTGTCTGGAGCCTTTGCATGTTCAGCATCCGTCGAGAATTCg                >  1:1194/1‑149 (MQ=255)                                                                                                                              gAAGATGAAGTGCTTCGACTGCGAGTCGAAGATATCAGCTGGCAGGGCCAGGGCGATCAGCATGAGCAGGCTCATGTAGAGCAGCCAC‑CCGGCGCACTGCAACAGCACTGTCTGGAGCCTTTGCATGTTCAGCATCCGTCGAGAATTCg                >  7:216222/1‑149 (MQ=255)                                                                                                                              gAAGATGAAGTGCTTCGACTGCGAGTCGAAGATATCAGCTGGCAGGGCCAGGGCGATCAGCATGAGCAGGCTCATGTAGAGCAGCCAC‑CCGGCGCACTGCAACAGCACTGTCTGGAGCCTTTGCATGTTCAGCATCCGTCGAGAATTCg                >  5:369680/1‑149 (MQ=255)                                                                                                                               aaGATGAAGTGCTTCGACTGCGAGTCGAAGATATCAGCTGGCAGGGCCAGGGCGATCAGCATGAGCAGGCTCATGTAGAGCAGCCAC‑CCGGCGCACTGCAACAGCACTGTCTGGAGCCTTTGCATGTTCAGCATCCGTCGAGAATTCgg               <  7:573/149‑1 (MQ=255)                                                                                                                                  aTGAAGTGCTTCGACTGCGAGTCGAAGATATCAGCTGGCAGGGCCAGGGCGATCAGCATGAGCAGGCTCATGTAGAGCAGCCAC‑CCGGCGCACTGCAACAGCACTGTCTGGAGCCTTTGCATGTTCAGCATCCGTCGAGAATTCGGGa             >  8:296060/1‑148 (MQ=255)                                                                                                                                       gttcTTCGACTGCGAGTCGAAGATATCAGCTGGCAGGGCCAGGGCGATCAGCATGAGCAGGCTCATGTAGAGCAGCCAC‑CCGGCGCACTGCAACAGCACTGTCTGGAGCCTTTGCATGTTCAGCATCCGTCGAGAATTCGGGAAAGGGt       <  8:104056/146‑1 (MQ=255)                                                                                                                                       gTGCTTCGACTGCGAGTCGAAGATATCAGCTGGCAGGGCCAGGGCGATCAGCATGAGCAGGCTCATGTAGAGCAGCCAC‑CCGGCGCACTGCAACAGCACTGTCTGGAGCCTTTGCATGTTCAGCATCCGTCGAGAATTCGGGAAAGGGt       >  3:234570/1‑149 (MQ=255)                                                                                                                                       gTGCTTCGACTGCGAGTCGAAGATATCAGCTGGCAGGGCCAGGGCGATCAGCATGAGCAGGCTCATGTAGAGCAGCCAC‑CCGGCGCACTGCAACAGCACTGTCTGGAGCCTTTGCATGTTCAGCATCCGTCGAGAATTCGGGAAAGGGt       <  3:304773/149‑1 (MQ=255)                                                                                                                                        tGCTTCGACTGCGAGTCGAAGATATCAGCTGGCAGGGCCAGGGCGATCAGCATGAGCAGGCTCATGTAGAGCAGCCAC‑CCGGCGCACTGCAACAGCACTGTCTGGAGCCTTTGCATGTTCAGc                                <  7:263992/123‑1 (MQ=255)                                                                                                                                        tGCTTCGACTGCGAGTCGAAGATATCAGCTGGCAGGGCCAGGGCGATCAGCATGAGCAGGCTCATGTAGAGCAGCCAC‑CCGGCGCACTGCAACAGCACTGTCTGGAGCCTTTGCATGTTCAGc                                >  8:263992/1‑123 (MQ=255)                                                                                                                                          cTTCGACTGCGAGTCGAAGATATCAGCTGGCAGGGCCAGGGCGATCAGCATGAGCAGGCTCATGTAGAGCAGCCAC‑CCGGCGCACTGCAACAGCACTGTCTGGAGCCTTTGCATGTTCAGCATCCGTCGAGAATTCGGGAAAGGGTggg    >  7:267927/1‑149 (MQ=255)                                                                                                                                          cTTCGACTGCGAGTCGAAGATATCAGCTGGCAGGGCCAGGGCGATCAGCATGAGCAGGCTCATGTAGAGCAGCCAC‑CCGGCGCACTGCAACAGCACTGTCTGGAGCCTTTGCATGTTCAGCATCCGTCGAGAATTCGGGAAAGGGTggg    >  2:288657/1‑149 (MQ=255)                                                                                                                                          cTTCGACTCGCAGTCGAAGATATCAGCTGGCAGGGCCAGGGCGATCAGCATGAGCAGGCTCATGTAGAGCAGCCAC‑CCGGCGCACTGCAACAGCACTGTCTGGAGCCTTTGCATGTTCAGCATCCGTCGAGAATTCGGGAAAg          >  8:24916/1‑143 (MQ=255)                                                                                                                                          cTTCGACTCGCAGTCGAAGATATCAGCTGGCAGGGCCAGGGCGATCAGCATGAGCAGGCTCATGTAGAGCAGCCAC‑CCGGCGCACTGCAACAGCACTGTCTGGAGCCTTTGCATGTTCAGCATCCGTCGAGAATTCGGGAAAg          <  7:24916/143‑1 (MQ=255)                                                                                                                                           ttCGACTCGCAGTCGAAGATATCAGCTGGCAGGGCCAGGGCGATCAGCATGAGCAGGCTCATGTAGAGCAGCCAC‑CCGGCGCACTGCAAAAGCACTGTCTGGAGCCTTTGCATGTTCAGCATCCGTCGAGAATTCGGGAAAGGGTggg    >  5:272644/1‑148 (MQ=255)                                                                                                                                            tCGACTGCGAGTCGAAGATATCAGCTGGCAGGGCCAGGGCGATCAGCATGAGCAGGCTCATGTAGAGCAGCCAC‑CCGGCGCACTGCAACAGCACTGTCTGGAGCCTTTGCATGTTc                                   >  1:97234/1‑116 (MQ=255)                                                                                                                                            tCGACTGCGAGTCGAAGATATCAGCTGGCAGGGCCAGGGCGATCAGCATGAGCAGGCTCATGTAGAGCAGCCAC‑CCGGCGCACTGCAACAGCACTGTCTGGAGCCTTTGCATGTTc                                   <  2:97234/116‑1 (MQ=255)                                                                                                                                             cGACTGCGAGTCGAAGATATCAGCTGGCAGGGCCAGGGCGATCAGCATGAGCAGGCTCATGTAGAGCAGCGAC‑CCGGCGCACTGCAACAGCACTGTCTGGAGCCTTTGCATGTTCAGCATCCGTCGAGAATTCGGGAAAGGGTGGGCa  <  1:121942/148‑1 (MQ=255)                                                                                                                                              gACTGCGAGTCGAAGATATCAGCTGGCAGGGCCAGGGCGATCAGCATGAGCAGGCTCATGTAGAGCAGCCAC‑CCGGCGCACTGCAACAGCACTGTCTGGAGCCTTTGCATGTTCAGCATCCGTCGAGAATTCGGGAAAgg         <  5:329027/140‑1 (MQ=255)                                                                                                                                              gACTGCGAGTCGAAGATATCAGCTGGCAGGGCCAGGGCGATCAGCATGAGCAGGCTCATGTAGAGCAGCCAC‑CCGGCGCACTGCAACAGCACTGTCTGGAGCCTTTGCATGTTCAGCATCCGTCGAGAATTCGGGAAAgg         >  6:329027/1‑140 (MQ=255)                                                                                                                                                   |                                                                                                                                               GTGGCATTACCCATTTTCTGCACCTTGCGGCGCAGGTACGGGTACACGCCGTAGAGGAAGATCATGCCGCGGATGAAATGGGTGGCGCCCATGGAGTAGCGCCAGATGCCGACTGCGCCGACCAGGAAGATGAAGTGCTTCGACTGCGAGTCGAAGATATCAGCTGGCAGGGCCAGGGCGATCAGCATGAGCAGGCTCATGTAGAGCAGCCAC‑CCGGCGCACTGCAACAGCACTGTCTGGAGCCTTTGCATGTTCAGCATCCGTCGAGAATTCGGGAAAGGGTGGGCA  >  NC_002947/1472567‑1472854

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑