Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,487,101 C→T 8.5% intergenic (+117/+13) yhdZ → / ← degS amino acid ABC transporter ATP‑binding protein/serine endoprotease DegS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,487,1010CT 8.5% 130.0 / 2.9 47intergenic (+117/+13)yhdZ/degSamino acid ABC transporter ATP‑binding protein/serine endoprotease DegS
Reads supporting (aligned to +/- strand):  ref base C (27/16);  new base T (2/2);  total (29/18)
Fisher's exact test for biased strand distribution p-value = 6.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.73e-01

GTTCCTCAGCCAGATCCTGCATTGATGCTGTTTGGGCTGCGCGGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTAGCGCTGGCTTTGGAGCCTGCGTAATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCAACGCGGCCCTGGGTTTATTTCTCTTCCTGGCTGGCAGCCGGCGCCGGTGGCGGCCGCAGCCCCACCTCGGCGATCAGCTTCAACTGCTGCCCGTTGCGCATCACCTCGATGGTTATCTTCTCGTTGGGCTTGATCCGCGCTACCTGGTT  >  NC_002947/1486960‑1487248
                                                                                                                                             |                                                                                                                                                   
gttCCTCAGCCAGATCCTGCATTGATGCTGTTTGGGCTGCGCGGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTAGCGCTGGCTTTGGAGCCTGCGTAATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCAACGCGGcc                                                                                                                                               <  1:46761/148‑1 (MQ=255)
   ccTCAGCCAGATCCTGCATTGATGCTGTTTGGGCTGCGCGGCAGCCCTTCGCGGGCACGCCCCCTACCCCAGGTTTAGCGCTGGGGTTGGAGCCTGCGTAATTACTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCAACGCGGCCCggg                                                                                                                                           >  3:178971/1‑149 (MQ=39)
       aGCCAGATCCTGCATTGATGCTGTTTGGGCTGCGCGGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTAGCGCTGGCTTTGGAGCCTGCGTAATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGTTGCGGCCCttcgcgg                                                                                                                                       <  7:100055/149‑7 (MQ=38)
               ccTCCATCGATGCTGTTTGGGCTGCGCGGCAGCCCTTCGCGGGCCCGCCCGCTCCCACAGGTTTAGCGCTGGCTTTGGCGCCTGCGTAATTCCTGGGGGCGCGGGCGTGCCCGCGAAGGGCCGCAACGCGGCCCTGGGTTTATTTctct                                                                                                                               <  4:18222/149‑1 (MQ=39)
                cTGCATTGATGCTGTTTGGGCTGCGCGGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTAGCGCTGGCTTTGGAGCCTGCGTAATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCAACGCGGCCCTGGGTTTATTTctct                                                                                                                               >  1:90200/1‑148 (MQ=255)
                             gTTTGGGCTGCGCGGCAGCCCTTCGCGGGCACGCCCGCTCCCCCAGGTTTAGGGCTGGCGTTGGAGCCTGCGTTATTCCTGGGGGCGCGGGCGGGCCCGCGAACGGCCGCCACGCGGCCCTGGGTTTATTTGTCTTCCTGGCTGGCAGc                                                                                                                 >  1:315230/1‑149 (MQ=38)
                                  ggCTGCGCGGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTTGCGCTGGCGTTGGAGCCTGCGTAATTGCTGTGGGCGCGGGCGTGCCCGCGAAGGGCCGCAACGCGGCCCGTGGGTTATTTGTCTTGCTGGCTGGCAGCCGgcg                                                                                                            >  1:200595/1‑149 (MQ=37)
                                                           aCGCCCGCTCCCACAGGTTTAGCGCTGGCTTTGGAGCCTGCGTAATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCAACGCGGCCCTGGGTTTATTTCTCTTCCTGGCTGGCAGCCGGCGCCGGTGGCGGCCGCAGCCCCACCTc                                                                                   >  7:339359/1‑149 (MQ=255)
                                                             gCCCGCTCCCACAGGTTTAGCGCTGGCTTTGGAGCCTGCGTAATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCAACGCGGCCCTGGGTTTATTTCTCTTCCTGGCTGGCAGCCGGCGCCGGTGGCGGCCGCAGCCCCACCTCgg                                                                                 >  3:301410/1‑149 (MQ=255)
                                                             gCCCGCTCCCACAGGTTTAGCGCTGGCTTTGGAGCCTGCGTAATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCAACGCGGCCCTGGGTTTATTTCTCTTCCTGGCTGGCAGCCGGCGCCGGTGGCGGCCGCAGCCCCACCTCgg                                                                                 >  4:109752/1‑149 (MQ=255)
                                                               ccGCTCCCACAGGTTTAGCGCTGGCTTTGGAGCCTGCGTAATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCAACGCGGCCCTGGGTTTATTTCTCTTCCTGGCTGGCAGCCGGCGCCGGTGGCGGCCGCAg                                                                                            >  7:21598/1‑136 (MQ=255)
                                                               ccGCTCCCACAGGTTTAGCGCTGGCTTTGGAGCCTGCGTAATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCAACGCGGCCCTGGGTTTATTTCTCTTCCTGGCTGGCAGCCGGCGCCGGTGGCGGCCGCAg                                                                                            <  8:21598/136‑1 (MQ=255)
                                                                     ccACAGGTTTAGCGCTGGCTTTGGAGCCTGCGTAATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCAACGCGGCCCTGGGTTTATTTCTCTTCCTGGCTGGCAGCCGGCGCCg                                                                                                         <  5:384647/117‑1 (MQ=255)
                                                                     ccACAGGTTTAGCGCTGGCTTTGGAGCCTGCGTAATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCAACGCGGCCCTGGGTTTATTTCTCTTCCTGGCTGGCAGCCGGCGCCg                                                                                                         >  6:384647/1‑117 (MQ=255)
                                                                      cacaGGTTTAGCGCTGGCTTTGGAGCCTGCGTAATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCAACGCGGCCCTGGGTTTATTTCTCTTCCTGGCTGGCAGCCGGCGCCGGTGGCGGCCGCAGCCCCACCTCGGCGATCAGCt                                                                        >  2:200549/1‑149 (MQ=255)
                                                                       acaGGTTTAGCGCTGGCTTTGGAGCCTGCGTAATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCAACGCGGCCCTGGGTTTATTTCTCTTCCTGGCTGGCAGCCGGCGCCGGTGGCGGCCGCAGCCCCACCTCGGCGATCAGCtt                                                                       >  3:46592/1‑149 (MQ=255)
                                                                       acaGGTTTAGCGCTGGCTTTGGAGCCTGCGTAATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCAACGCGGCCCTGGGTTTATTTCTCTTCCTGGCTGGCAGCCGGCGCCGGTGGCGGCCGCAGCCCCACCTCGGCGATCAGCtt                                                                       >  7:188883/1‑149 (MQ=255)
                                                                       acaGGTTTAGCGCTGGCTTTGGAGCCTGCGTAATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCAACGCGGCCCTGGGTTTATTTCTCTTCCTGGCTGGCAGCCGGCGCCGGTGGCGGCCGCAGCCCCACCTCGGCGATCAGCt                                                                        >  5:231782/1‑148 (MQ=255)
                                                                       acaGGTTTAGCGCTGGCTTTGGAGCCTGCGTAATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCAACGCGGCCCTGGGTTTATTTCTCTTCCTGGCTGGCAGCCGGCGCCGGTGGCGGCCGCAGCCCCACCTAGGCGATCAGCtt                                                                       >  4:26740/1‑149 (MQ=255)
                                                                        caGGTTTAGCGCTGGCTTTGGAGCCTGCGTAATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCAACGCGGCCCTGGGTTTATTTCTCTTCCTGGCTGGCAGCCGGCGCCGGTGGCGGCCGCAGCCCCACCTCGGCGATCAGCtt                                                                       >  2:368154/1‑148 (MQ=255)
                                                                            tttAGCGCTGGCTTTGGAGCCTGCGTAATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCAACGCGGCCCTGGGTTTATTTCTCTTCCTGGCTGGCAGCCGGCGCCGGTGGCGGCCGCAGCCCCACCTCGGCGATCAGCTTCAAct                                                                  <  4:301410/149‑1 (MQ=255)
                                                                               aGCGCTGGCTTTGGAGCCTGCGTAATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCAACGCGGCCCTGGGTTTATTTCTCTTCCTGGCTGGCAGACGGCGCCGGTGGCGGCCGCAGCCCCACCTCGGCGATCAGCTTCAActgct                                                               >  5:89631/1‑149 (MQ=255)
                                                                                 cggtGGCTTTGTAGCCGGCGTAATTCTGGTGGGTGGGGGCGTGCCCGCGAAGGGCCGCAACGCGGCCCTGGGTTTATTTCTCTTCCTGGCTGGCAGCCGGCGCCGGTGGCGGCCGCAGCCCCACCTCGGCGATCAGCTTCAACtgctgc                                                             <  3:58145/146‑1 (MQ=255)
                                                                                  gcTGGCTTTGGAGCCTGCGTAATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCAACGCGGCCCTGGGTTTATTTCTCTTCCTGGCTGGCAGCCGGCGCCGGTGGCGGCCg                                                                                               >  7:261936/1‑114 (MQ=255)
                                                                                  gcTGGCTTTGGAGCCTGCGTAATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCAACGCGGCCCTGGGTTTATTTCTCTTCCTGGCTGGCAGCCGGCGCCGGTGGCGGCCg                                                                                               <  8:261936/114‑1 (MQ=255)
                                                                                     ggCTTTGGAGCCTGCGTAATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCAACGCGGCCCTGGGTTTATTTCTCTTCCTGGCTGGCAGCCGGCGCCGGTGGCGGCCGCAGCCCCACCTCGGCGATCAGCTTCAACTGCTGCCCGt                                                         >  8:39686/1‑149 (MQ=255)
                                                                                     ggCTTTGGAGCCTGCGTAATTCCTGTGGGAGCCGGCGTGCCCGCGAAGGGCCGCAACGCCGCCCTGGGTTTATTTGTCTTCCTTGCTGGCAGCCGGCGCCGGTGGCGGCCGCAGCCCCCCCTCGGCGATGAGCATCAACTGCTGCCCGt                                                         >  1:29320/1‑149 (MQ=255)
                                                                                      gCTTTGGAGCCTGCGTAATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCAACGCGGCCCTGGGTTTATTTCTCTTCCTGGCTGGCa                                                                                                                   <  3:347941/90‑1 (MQ=255)
                                                                                      gCTTTGGAGCCTGCGTAATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCAACGCGGCCCTGGGTTTATTTCTCTTCCTGGCTGGCa                                                                                                                   >  4:347941/1‑90 (MQ=255)
                                                                                           gccgCCGGCGTAATGCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCAACGCGGCCCTTGGTTTATTTCTCTTCCTGGCTGGCAGCCGGCGCCGGTGGCGGCCGCAGCCCCACCTCGGCGATCAGCTTCAACTGCTGCCCGTTgcgc                                                    <  2:200595/145‑1 (MQ=255)
                                                                                              gCCTGCGTAATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCAACGCGGCCCTGGGTTTATTTCTCTTCCTGGCTGGCAGCCGGCGCCGGTGGCGGCCGCAGCCCCACCTCGGCGATCAGCTTCAACTGCTGCCCGTTGCGCATCa                                                <  1:368154/149‑1 (MQ=255)
                                                                                                          ccTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCAACGCGGCCCTGGGTTTATTTCTCTTCCTGGCTGGCAGCCGGCGCCGGTGGCGGCCGCAGCCCCACCTCGGCGATCAGCTTCAACTGCTGCCCGTTGCGCATCACCTCGATGGtt                                     <  2:315230/148‑1 (MQ=255)
                                                                                                              tgGGAGCGGGCGTGCCCGCGAAGGGCCGCAACGCGGCCCTGGGTTTATTTCTCTTCCTGGCTGGCAGCCGGCGCCGGTGGCGGCCGCAGCCCCACCTCGGCGATCAGCTTCAACTGCTGCCCGTTGCGCATCACCTCGATGGTTAtctt                                >  4:321440/1‑149 (MQ=255)
                                                                                                               gggAGCGGGCGTGCCCGCGAAGGGCCGCAACGCGGCCCTGGGTTTATTTCTCTTCCTGGCTGGCAGCCGGCGCCGGTGGCGGCCGCAGCCCCACCTCGGCGATCAGCTTCAACTGCTGCCCGTTGCGCATCACCTCGATGGTTAtcttc                               >  3:168184/1‑149 (MQ=255)
                                                                                                                     gggCGTGCCCGCGAAGGGCCGCAACGCGGCCCTGGGTTTATTTCTCTTCCTGGCTGGCAGCCGGCGCCGGTGGCGGCCGCAGCCCCACCTCGGCGATCAGCTTCAACTGCTGCCCGTTGCGCATCACCTCGATGGTTATCTTCTCGTTg                         <  4:178971/149‑1 (MQ=255)
                                                                                                                     gggCGTGCCCGCGAAGGGCCGCAACGCGGCCCTGGGTTTATTTCTCTTCCTGGCTGGCAGCCGGCGCCGGTGGCGGCCGCAGCCCCACCTCGGCGATCAGCTTCAACTGCTGCCCGTTGCGCATCACCTCGATGGTTATCTTCTCGTTg                         <  2:90200/149‑1 (MQ=255)
                                                                                                                       gCGTGCCCGCGAAGGGCCGCAACGCGGCCCTGGGTTTATTTCTCTTCCTGGCTGGCAGCCGGCGCCGGTGGCGGCCGCAGCCCCACCTCGGCGATCAGCTTCAACTGCTGCCCGTTGCGCATCACCTCGATGGTTATCTTCTCGTTggg                       <  1:200549/149‑1 (MQ=255)
                                                                                                                           gCCCGCGAAGGGCCGCAACGCGGCCCTGGGTTTATTTCTCTTCCTGGCTGGCAGCCGGCGCCGGTGGCGGCCGCAGCCCCACCTCg                                                                                  >  8:5539/1‑86 (MQ=255)
                                                                                                                               acccaGGGCCGCGTTGCGGCCCTTGGTTTATTTCTCTTCCTGGCTGGCAGCCGGCGCCGGTGGCGGCCGCAGCCCCACCTCGGCGATCAGCTTCAACTGCTGCCCGTTGCGCATCACCTCGATGGTTATCTTCTCGTTgg                        >  4:102383/5‑140 (MQ=255)
                                                                                                                               acccaGGGCCGCGTTGCGGCCCTTGGTTTATTTCTCTTCCTGGCTGGCAGCCGGCGCCGGTGGCGGCCGCAGCCCCACCTCGGCGATCAGCTTCAACTGCTGCCCGTTGCGCATCACCTCGATGGTTATCTTCTCGTTgg                        <  3:102383/136‑1 (MQ=255)
                                                                                                                               acccaGGGCCGCGTTGCGGCCCTGGGTTTATTTCTCTTCCTGGCTGGCAGCCGGCGCCGGTGGCGGCCGCAGCCCCACCTCGGCGATCAGCTTCAACTGCTGCCCGTTGCGCATCACCTCGATGGTTATCTTCTCGTTGGGCTTGATcc               >  5:209098/5‑149 (MQ=255)
                                                                                                                                 gAAGGGCCGCAACGCGGCCCTGGGTTTATTTCTCTTCCTGGCTGGCAGCCGGCGCCGGTGGCGGCCGCAGCCCCACCTAGGCGATCAGCTTCAACTGCTGCCCGTTGCGCATCACCTCGATGGTTATCTTCTCGTTGGGCTTGATCcgc             <  3:26740/149‑1 (MQ=255)
                                                                                                                                       ccGCAACGCGGCCCTGGGTTTATTTCTCTTCCTGGCTGGCAGCCGGCGCCGGTGGCGGCCGCAGCCCCACCTCGGCGATCAGCTTCAACTGCTGCCCGTTGCGCATCACCTCGATGGTTATCTTCTCGTTGGGCTTGATCCGCGCTAcc       >  8:128926/1‑149 (MQ=255)
                                                                                                                                        cGCAACGCGGCCCTGGGTTTATTTCTCTTCCTGGCTGGCAGCCGGCGCCGGTGGCGGCCGCAGc                                                                                           <  2:154055/64‑1 (MQ=255)
                                                                                                                                        cGCAACGCGGCCCTGGGTTTATTTCTCTTCCTGGCTGGCAGCCGGCGCCGGTGGCGGCCGCAGc                                                                                           >  1:154055/1‑64 (MQ=255)
                                                                                                                                        cGCAACGCGGCCCTGGGTTTATTTCTCTTCCTGGCTGGCAGCCGGCGCCGGTGGCGGCCGCAGCCCCACCTCGGCGATCAGCTTCAACTGCTGCCCGTTGCGCATCACCTCGATGGTTATCTTCTCGTTGGGCTTGATCCGCGCTACCt      >  1:224577/1‑149 (MQ=255)
                                                                                                                                         gCAACGCGGCCCTGGGTTTATTTCTCTTCCTGGCTGGCAGCCGGCGCCGGTGGCGGCCGCAGCCCCACCTCGGCGATCAGCTTCAACTGCTGCCCGTTGCGCATCACCTCGATGGTTATCTTCTCGTTGGGCTTGCTCCGCGCTTCCTg     >  6:366341/1‑149 (MQ=255)
                                                                                                                                             cgcgGCCCTGGGTTTATTTCTCTTCCTGGCTGGCAGCCGGCGCCGGTGGCGGCCGCAGCCCCACCTCGGCGATCAGCTTCAACTGCTGCCCGTTGCGCATCACCTCGATGGTTATCTTCTCGTTGGGCTTGATCCGCGCTACCTGGtt  >  1:345996/1‑148 (MQ=255)
                                                                                                                                             |                                                                                                                                                   
GTTCCTCAGCCAGATCCTGCATTGATGCTGTTTGGGCTGCGCGGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTAGCGCTGGCTTTGGAGCCTGCGTAATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCAACGCGGCCCTGGGTTTATTTCTCTTCCTGGCTGGCAGCCGGCGCCGGTGGCGGCCGCAGCCCCACCTCGGCGATCAGCTTCAACTGCTGCCCGTTGCGCATCACCTCGATGGTTATCTTCTCGTTGGGCTTGATCCGCGCTACCTGGTT  >  NC_002947/1486960‑1487248

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑