Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,527,744 G→A 8.0% E29K (GAA→AAA)  ftsA → cell division protein FtsA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,527,7440GA 8.0% 141.9 / 2.4 50E29K (GAA→AAA) ftsAcell division protein FtsA
Reads supporting (aligned to +/- strand):  ref base G (22/24);  new base A (2/2);  total (24/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01

CGATTGCACCGACGACGGCCCAACCCGCCGTTGCGAAGAATTAGAGAGAGGCAGGACCATGGCAAACGCGCATAGCGGCAAAATGATCGTCGGGCTGGACATTGGCACCT‑CCAAGGTGGTGGCGCTGGTGGGCGAAGTGGGCGAAGACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGCCTGAAAAAAGGCGTGGTGGTGAACATCGAGTCCACCGTGCAGTCGATCCAGCGCGCCGTGGAAGAGGCGCAGCTGATGGCCGGCTGCCGTATCCA  >  NC_002947/1527602‑1527889
                                                                                                                                               |                                                                                                                                                 
cGATTGCACCGACGACGGCCCAACCCGCCGTTGCGAAGAATTAGAGAGAGGCAGGACCATGGCAAACGCGCATAGCGGCAAAATGATCGTCGGGCTGGACATTGGCACCT‑CCAAGGTGGTGGCGCTGGTGGGCGAAGTGGGCGAAGACg                                                                                                                                             >  8:123214/1‑149 (MQ=255)
cGATTGCACCGACGACGGCCCAACCCGCCGTTGCGAAGAATTAGAGAGAGGCAGGACCATGGCAAACGCGCATAGCGGCAAAATGATCGTCGGGCTGGACATTGGCACCT‑CCAAGGTGGTGGCGCTGGTGGGCGAAGTGGGCGAAGACg                                                                                                                                             <  2:215681/149‑1 (MQ=255)
  aTTGCACCGACGACGGCCCAACCCGCCGTTGCGAAGAATTAGAGAGAGGCAGGACCATGGCAAACGCGCATAGCGGCAAAATGATCGTCGGGCTGGACATTGGCACCT‑CCAAGGTGGTGGCGCTGGTGGGCGACGTGGGCGAAGACgg                                                                                                                                            >  8:337599/1‑148 (MQ=255)
       aCCGACGACGGCCCAACCCGCCGTTGCGAAGAATTAGAGAGAGGCAGGACCATGGCAAACGCGCATAGCGGCAAAATGATCGTCGGGCTGGACATTGGCACCT‑CCAAGGTGGTGGCGCTGGTGGGCGAAGTGGGCGAAGACGGCACCCt                                                                                                                                      >  7:327326/1‑149 (MQ=255)
        ccGACGACGGACCAACCCGCCGTTGCGAAGAATTAGAGAGAGGCAGGACCATGGCAAACGCGCATAGCGGCAAAATGATCGTCGGGCTGGACATTGGCACCT‑CCAAGGTGGTGGCGCTGGTGGGCGAAGTGGGCGAAGACGGCACCCTg                                                                                                                                     <  7:233601/149‑1 (MQ=255)
                  cccAACCCGCCGTTGCGAAGAATTAGAGAGAGGCAGGACCATGGCAAACGCGCATAGCGGCAAAATGATCGTCGGGCTGGACATTGGCACCT‑CCAAGGTGGTGGCGCTGGTGGGCGAAGTGGGCGAAGACGGCACCCTGGAAAtcgtcg                                                                                                                           <  8:327326/149‑1 (MQ=255)
                    cAACCCGCCGTTGCGAAGAATTAGAGAGAGGCAGGACCATGGCAAACGCGCATAGCGGCAAAATGATCGTCGGGCTGGACATTGGCACCTCCCAAGGTGGTGGCGCTGGTGGGCGAAGTGGGCGAAGACGGCACCCTGGAAATCGTCgg                                                                                                                          <  1:108770/149‑1 (MQ=255)
                      aCCCGCCGTTGCGAAGAATTAGAGAGAGGCAGGACCATGGCAAACGCGCATAGCGGCAAAATGATCGTCGGGCTGGACATTGGCACCT‑CCAAGGTGGTGGCGCTGGTGGGCGAAGTGGGCGAAGACGGCACCCTGGAAATCGTCGGGAt                                                                                                                       <  6:168446/149‑1 (MQ=255)
                            cgTTGCGAAGAATTAGAGAGAGGCAGGACCATGGCAAACGCGCATAGCGGCAAAATGATCGTCGGGCTGGACATTGGCACCT‑CCAAGGTGGTGGCGCTGGTGGGCGAAGTGGGCGAAGACGGCACCCTGGAAATCGTCGGGATCGGTAc                                                                                                                 <  7:123214/149‑1 (MQ=255)
                                  gaagaaTTAGAGAGAGGCAGGACCATGGCAAACGCGCATAGCGGCAAAATGATCGTCGGGCTGGACATTGGCACCT‑CCAAGGTGGTGGCGCTGGTGGGCGAAGTGGGCAAAGACGGCACCCTGGAAATCGTCGGGATCGGTACCCATcc                                                                                                           >  2:184684/1‑149 (MQ=255)
                                            agagagGCAGGACCATGGCAAACGCGCATAGCGGCAAAATGATCGTCGGGCTGGACATTGGCACCT‑CCAAGGTGGTGGCGCTGGTGGGCGAAGTGGGCGAAGACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGc                                                                                                 >  8:100685/1‑149 (MQ=255)
                                            agagagGCAGGACCATGGCAAACGCGCATAGCGGCAAAATGATCGTCGGGCTGGACATTGGCACCT‑CCAAGGTGGTGGCGCTGGTGGGCGAAGTGGGCGAAGACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGc                                                                                                 >  5:239553/1‑149 (MQ=255)
                                            agagagGCAGGACCATGGCAAACGCGCATAGCGGCAAAATGATCGTCGGGCTGGACATTGGCACCT‑CCAAGGTGGTGGCGCTGGTGGGCGAAGTGGGCAAAGACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGc                                                                                                 <  1:184684/149‑1 (MQ=255)
                                                  gCAGGACCATTGCAAACGCGCATAGCGGCAAAATGATCGTCCGGCTGGACATTGGCACCT‑CCAAGATGGTGGCGCTGGTGGGCGAAGTGGGCGAAGACGGCACACTGGAAATCGTCGGGATACGTACCCATCCGTACCGTGGCCGGaa                                                                                            >  4:305706/1‑148 (MQ=255)
                                                  gCAGGACCATGGCAAACGCGCATAGCGGCAAAATGATCGTCGGGCTGGACATTGGCACCT‑CCAAGGTGGTGGCGCTGGTGGGCGAAGTGGGCGAAGACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGCCTGaa                                                                                            >  6:11910/1‑148 (MQ=255)
                                                    aGGACCATGGCAAACGCGCATAGCGGCAAAATGATCGTCGGGCTGGACATTGGCACCT‑CCAAGGTGGTGGCGCTGGTGGGCGAAGTGGGCGAAGACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGCCTGaaaa                                                                                          >  4:157073/1‑148 (MQ=255)
                                                    aGGACCATGGCAAACGCGCATAGCGGCAAAATGATCGTCGGGCTGGACATTGGCACCT‑CCAAGGTGGTGGCGCTGGTGGGCGAAGTGGGCAAAGACGGCACCCTGGAAATCGTCGGGATCGGTA‑CCATCCGTCCCGTGGCCTGaaaa                                                                                          >  3:197719/1‑147 (MQ=255)
                                                     ggACCATGGCAAACGCGCATAGCGGCAAAATGATCGTCGGGCTGGACATTGGCACCT‑CCAAGGTGGTGGCGCTGGTGGGCGAAGTGGGCGAAGACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGCCTGaaaaaa                                                                                        <  6:132444/149‑1 (MQ=255)
                                                     ggACCATGGCAAACGCGCATAGCGGCAAAATGATCGTCGGGCTGGACATTGGCACCT‑CCAAGGTGGTGGCGCTGGTGGGCGAAGTGGGCGAAGACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGCCTGaaaaaa                                                                                        <  4:37406/149‑1 (MQ=255)
                                                      gACCATGGCAAACGCGCATAGCGGCAAAATGATCGTCGGGCTGGACATTGGCACCT‑CCAAGGTGGTGGCGCTGGTGGGCGAAGTGGGCGAAGACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGCCTGAAAAAAg                                                                                       <  4:63163/149‑1 (MQ=255)
                                                        ccATGGCAAACGCGCATAGCGGCAAAATGATCGTCGGGCTGGACATTGGCACCT‑CCAAGGTGGTGGCGCTGGTGGGCGAAGTGGGCGAAGACGGCACCATGGAAATCGTCGGGATCGGTACCCATCCGTCCCCTGGCCTGAAAAAAGGc                                                                                     >  7:300748/1‑149 (MQ=255)
                                                         cATGGCAAACGCGCATAGCGGCAAAATGATCGTCGGGCTGGACATTGGCACCT‑CCAAGGTGGTGGCGCTGGTGGGCGAAGTGGGCGAAGACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGCCTGAAAAAAGGCg                                                                                    >  8:134508/1‑149 (MQ=255)
                                                           tGGCAAACGCGCATAGCGGCAAAATGATCGTCGGGCTGGACATTGGCACCT‑CCAAGGTGGTGGCGCTGGTGGGCGAAGTGGGCGAAGACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGCCTGAAAAAAGGCgtg                                                                                  <  6:126523/149‑1 (MQ=255)
                                                              cAAACGCGCATAGCGGCAAAATGATCGTCGGGCTGGACATTGGCACCT‑CCAAGGTGGTGGCGCTGGTGGGCGAAGTGGGCGAAGACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGCCTGAAAAAAGGCgtggtg                                                                               <  7:337599/149‑1 (MQ=255)
                                                                aaCGCTCATAGCGGCAAACTGAGCGTCGGGCTGGACATTGGCAACT‑CCAAGGTGTTGGCGCTGGTGGGCGACGTGGGCGAAGACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGCCTGAAAAAAGGCgtggtggt                                                                             <  4:333199/149‑1 (MQ=255)
                                                                   gcgcATAGCGGCAAAATGATCGTCGGGCTGGACATTGGCACCT‑CCAAGGTGGTGGCGCTGGTGGGCGAAGTGGGCGAAGACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGCCTGAAAAAAGGCGTGGTGGTGaa                                                                          >  6:32751/1‑149 (MQ=255)
                                                                       aTAGCGGCAAAATGATCGTCGGGCTGGACATTGGCACCT‑CCAAGGTGGTGGCGCTGGTGGGCGAAGTGGGCGAAGACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGCCTGAAAAAAGGCGTGGTGGTGAACATc                                                                      <  7:134508/149‑1 (MQ=255)
                                                                       aTAGCGGCAAAATGATCGTCGGGCTGGACATTGGCACCT‑CCAAGGTGGTGGCGCTGGTGGGCGAAGTGGGCAAAGACGGCACCCTGGAAATCGTCGGGATCGGTA‑CCATCCGTCCCGTGGCCTGAAAAAAGGCGTGGTGGTGAACATCg                                                                     <  4:197719/149‑1 (MQ=255)
                                                                             gCAAAATGATCGTCGGGCTGGACATTGGCACCT‑CCAAGGTGGTGGCGCTGGTGGGCGAAGTGGGCGAAGACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGCCTGAAAAAAGGCgtg                                                                                  >  8:38243/1‑131 (MQ=255)
                                                                             gCAAAATGATCGTCGGGCTGGACATTGGCACCT‑CCAAGGTGGTGGCGCTGGTGGGCGAAGTGGGCGAAGACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGCCTGAAAAAAGGCgtg                                                                                  <  7:38243/131‑1 (MQ=255)
                                                                               aaaaTGATCGTCGGGCTGGACATTGGCACCT‑CCAAGGTGGTGGCGCTGGTGGGCGAAGTGGGCGAAGACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGCCTGAAAAAAGGCGTGGTGGTGAACATCGAGTCCAc                                                              >  3:136225/1‑149 (MQ=255)
                                                                                aaaTGATCGTCGGGCTGGACATTGGCACCT‑CCAAGGTGGTGGCGCTGGTGGGCGAAGTGGGCGAAGACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGCCTGAAAAAAGGCGTGGTGGTGAACATCGAGTCCAcc                                                             <  3:351427/149‑1 (MQ=255)
                                                                                  aTGATCGTCGGGCTGGACATTGGCACCT‑CCAAGGTGGTGGCGCTGGTGGGCGAAGTGGGCGAAGACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGCCTGAAAAAAGGCGTGGTGGTGAACATCGAGTCCACCGt                                                           <  3:305706/149‑1 (MQ=255)
                                                                                        gtcgGGCTGGACATTGGCACCT‑CCAAGGTGGTGGCGCTGGTGGGCGAAGTGGGCGAAGACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGCCTGAAAAAAGGCGTGGTGGTGAACATCGAGTCCACCGTGCAGTc                                                     >  4:96737/1‑149 (MQ=255)
                                                                                         tcgGGCTGGACATTGGCACCT‑CCAAGGTGGTGGCGCTGGTGGGCGAAGTGGGCGAAGACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGCCTGAAAAAAGGCGTGGTGGTGAACATCGAGTCCACCGTGCAGTc                                                     >  5:133148/1‑148 (MQ=255)
                                                                                                 gACATTGGCACCT‑CCAAGGTGGTGGCGCTGGTGGGCGAAGTGGGCGAAGACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGCCTGAAAAAAGGCGTGGTGGTGAACATCGAGTCCACCGTGCAGTCGATCCAg                                              <  3:157073/147‑1 (MQ=255)
                                                                                                   cATTGGCACCT‑CCAAGGTGCGGGCGCTGGTGGGCGAAGTGGGCGAAGACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGCCTGAAAAAAGGCGTGGTGGTGAACATCGAGTCCACCGTGCAGTCGATCCAgcgcg                                          <  2:277137/149‑1 (MQ=255)
                                                                                                    aTTGGCACCT‑CCAAGGTGGTGGCGCTGGTGGGCGAAGTGGGCGAAGACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGCCTGAAAAAAGGCGTGGTGGTGAACATCGAGTCCACCGTGCAGTCGATCCAgcgcgc                                         >  3:263440/1‑149 (MQ=255)
                                                                                                     ttGGCACCT‑CCAAGGTGGTGGCGCTGGTGGGCGAAGTGGGCGAAGACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGCCTGAAAAAAGGCGTGGTGGTGAACATCGAGTCCACCGTGCAGTCGATCCAGCGCGcc                                        >  1:64140/1‑149 (MQ=255)
                                                                                                      tGGCACCT‑CCAAGGTGGTGGCGCTGGTGGGCGAAGTGGGCGAAGACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGCCTGAAAAAAGGCGTGGTGGTGAAc                                                                         >  1:328648/1‑115 (MQ=255)
                                                                                                      tGGCACCT‑CCAAGGTGGTGGCGCTGGTGGGCGAAGTGGGCGAAGACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGCCTGAAAAAAGGCGTGGTGGTGAAc                                                                         <  2:328648/115‑1 (MQ=255)
                                                                                                        gCACCT‑CCAAGGTGGTGGCGCTGGTGGGCGAAGTGGGCGAAGACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGCCTGAAAAAAGGCGTGGTGGTGAACATCGAGTCCACCGTGCAGTCGATCCAGCGCGCCGTg                                     <  5:11910/149‑1 (MQ=255)
                                                                                                        gCACCT‑CCAAGGTGGTGGCGCTGGTGGGCGAAGTGGGCGAAGACGGCACACTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGCCTGAAAAAAGGCGTGGTGGTGAACATCGAGTCCACCGTGCAGTCGATCCAGCGCGCCGTg                                     >  4:314180/1‑149 (MQ=255)
                                                                                                         cACCT‑CCAAGGTGTTGGCGCTGGTGGGCGATGTGGGAGAAGAGGGCACCCTGGAAATCGTCGTTATCTGTATCCATCAGTCCCGTTGCCTGAAAAAAGGCGTGGTGGTGAACATCGAGTCCTCCGTGCAGTCGATCCAGGGGGCAGTgg                                    <  2:303483/149‑1 (MQ=255)
                                                                                                             t‑CCAAGGTGGTGGCGCTGGTGGGCGAAGTGGGCGAAGACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGCCTGAAAAAAGGCGTGGTGGTGAACATCGAGTCCACCGTGCAGTCGATCCAGCGCGCCGTgg                                    >  4:20303/1‑145 (MQ=255)
                                                                                                                cAAGGTGGTGGCGCTGGTGGGCGAAGTGGGCGAAGACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGCCTGAAAAAAGGCGTGGTGGTGAACATCGAGTCCACCGTGCAGTCGATCCAGCGCGCCGTGGAagag                               <  6:239553/148‑1 (MQ=255)
                                                                                                                cAAGGTGGTGGCGCTGGTGGGCGAAGTGGGCGAAGACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGCCTGAAAAAAGGCGTGGTGGTGAACATCGAGTCCACCGTGCAGTCGATCCAGCGCGCCGTGGAagag                               <  6:133148/148‑1 (MQ=255)
                                                                                                                cAAGGTGGTGGCGCTGGTGGGCGAAGTGGGCGAAGACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGCCTGAAAAAAGGCGTGGTGGTGAACATCGAGTCCACCGTGCAGTCGATCCAGCGCGCCGTGGAagag                               <  3:20303/148‑1 (MQ=255)
                                                                                                                        tggCGCTGGTGGGCGAAGTGGGCGAAGACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGCCTGAAAAAAGGCGTGGTGGTGAACATCGAGTCCACCGTGCAGTCGATCCAGCGCGCCGTGGAAGAGGCGCAGCt                       >  2:20087/1‑148 (MQ=255)
                                                                                                                         ggCGCTGGTGGGCGAAGTGGGCGAAGACGGCACCCTGCAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGACAGAAAAAAGGCCTCGTGGTGAACATCGAGTCCACCGTGCAGTCGATCCAGCGCGCCGTGGAAGATGCGCAGCTGa                     >  2:19541/1‑149 (MQ=255)
                                                                                                                                gtgggcgaagtgggcgaaGACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGCCTGAAAAAAGGCGTGGTGGTGAACATCGAGTCCACCGTGCAGTCGATCCAGCGCGCCGTGGAAGAGGCGCAGCTGATGGCCgg              >  2:214711/1‑149 (MQ=255)
                                                                                                                                gtgggcgaagtgggcgaaGACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGCCTGAAAAAAGGCGTGGTGGTGAACATCGAGTCCACCGTGCAGTCGATCCAGCGCGCCGTGGAAGAGGCGCAGCTGATGGCCgg              <  2:64140/149‑1 (MQ=255)
                                                                                                                                     cgaagtgggcgaagACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGCCTGAAAAAAGGCGTGGTGGTGAACATCGAGTCCACCGTGCAGTCGATCCAGCGCGCCGTGGAAGAGGCGCAGCTGATGGCCGGCTGcc         <  6:363183/149‑1 (MQ=255)
                                                                                                                                      gaagtgggcgaagACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGCCTGAAAAAAGGCGTGGTGGTGAACATCGAGTCCACCGTGCAGTCGATCCAGCGCGCCGTGGAAGAGGCGCAGCTGATGGCCGGCTGCCg        <  1:20087/149‑1 (MQ=255)
                                                                                                                                              cgaagACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGCCTGAAAAAAGGCGTGGTGGTGAACATCGAGTCCACCCTGCAGTCGATCCAGCGCGCCGTGGAAGAGGCGCAGCTGATGGCCGGCTGCCGTATCCa  >  8:121361/1‑147 (MQ=255)
                                                                                                                                               |                                                                                                                                                 
CGATTGCACCGACGACGGCCCAACCCGCCGTTGCGAAGAATTAGAGAGAGGCAGGACCATGGCAAACGCGCATAGCGGCAAAATGATCGTCGGGCTGGACATTGGCACCT‑CCAAGGTGGTGGCGCTGGTGGGCGAAGTGGGCGAAGACGGCACCCTGGAAATCGTCGGGATCGGTACCCATCCGTCCCGTGGCCTGAAAAAAGGCGTGGTGGTGAACATCGAGTCCACCGTGCAGTCGATCCAGCGCGCCGTGGAAGAGGCGCAGCTGATGGCCGGCTGCCGTATCCA  >  NC_002947/1527602‑1527889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑