Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,543,816	C→T	6.1%	P207L (CCG→CTG)	mdfA →	multidrug MFS transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,543,816	0	C	T	6.1%	244.8 / 3.7	81	P207L (CCG→CTG)	mdfA	multidrug MFS transporter
Reads supporting (aligned to +/- strand):  ref base C (42/34);  new base T (3/2);  total (45/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACCTTGCTGCTGCAATGGCTGGACTGGCGCTGGCTGTTCGTGGCATTCGCCATCGGCGCGGTGCTGACCTGGCTGCTGCTGTACCGGTTGATGCCGGAAACCCTGGGCGTGGAGCGCCGTGACGGCTCGCGGCTGGCGTTCACGC‑CGATTCACCTGCTGCCACTGTTGGCGGGCTATGGCCAGTTGCTGGGCAACCGCCGCTTCGTTGCAGGCAGCGCCGCCCTTGGGTTGGTCGGATTGCCGCTGATTGGCTGGATTGGCCTGTCGCCGGTGCTGCTGATTCACGATGAGGG  >  NC_002947/1543670‑1543963                                                                                                                                                    |                                                                                                                                                    cACCTTGCTGCTGCAATGGCTGGACTGGCGCTGGCTGTTCGTGGCATTCGCCATCGGCGCGGTGCTGACCTGGCTGCTGCTGTACCGGTTGATGCCGGAAACCCTGGGCGTGGAGCGCCGTGACGGCTCGCGGCTGGCGTTCACGC‑CGa                                                                                                                                                   <  7:315537/149‑1 (MQ=255) cACCTTGCTGCTGCAATGGCTGGACTGGCGCTGGCTGTTCGTGGCATTCGCCATCGGCGCGGTGCTGACCTGGCTGCTGCTGTACCGGTTGATGCCGGAAACCCTGGGCGTGGAGCGCCGTGACGGCTCGCGGCTGGCGTTCACGC‑CGa                                                                                                                                                   >  1:76401/1‑149 (MQ=255)  aCCTTGCTGCTGCAATGGCTGGACTGGCGCTGGCTGTTCGTGGCATTCGCCATCGGCGCGGTGCTGACCTGGCTGCTGCTGTACCGGTTGATGCCGGAAACCCTGGGCGTGGAGCGCCGTGACGGATCGCGGCTGGCGTTACCGC‑CGAt                                                                                                                                                  >  4:277015/1‑149 (MQ=255)  aCCTTGCAGCTGCATTGGGTGGACTGGCGCTGGCGGTTCGTGGCATTCGCCATCGGCGCGGTGCTGACCTGGCTGCTGCTGTACCGGTTGATGCCGGAAACCCTGGGCGTGGAGCGCCGTGACGGCTCGCGGCTGGCGTTCACGC‑CGAt                                                                                                                                                  <  4:234923/149‑1 (MQ=255)   ccTTGCTGCTGCAATGGCTGGACTGGCGCTGGCTGTTCGTGGCATTCGCCATCGGCGCGGTGCTGACCTGGCTGCTGCTGTACCGGTTGATGCCGGAAACCCTGGGCGTGGAGCGCCGTGACGGCTCGCGGCTGGCGTTCACGC‑CGAtt                                                                                                                                                 >  7:82008/1‑149 (MQ=255)   ccTTGCTGCTGCAATGGCTGGACTGGCGCTGGCTGTTCGTGGCATTCGCCATCGGCGCGGTGCTGACCTGGCTGCTGCTGTACCGGTTGATGCCGGAAACCCTGGGCGTGGAGCGCCGTGACGGCTCGCGGCTGGCGTTCACGC‑CGAtt                                                                                                                                                 <  8:356697/149‑1 (MQ=255)    cTTGCTGATGCAATGGCTGGACTGGCGCTGGCTGTTCGTGGCATTCGCCATCGGCGCGGTGCTGACCTGGCTGCTGCTGTACCGGTTGATGCCGGAAACCCTGGGCGTGGAGCGCCGTGACGGCTCGCGGCTGGCGTTCACGC‑CGAtt                                                                                                                                                 >  5:228362/1‑148 (MQ=255)           ctgcAATGGCTGGACTGGCGCTGGCTGTTCGTGGCATTCGCCATCGGCGCGGTGCTGACCTGGCTGCTGCTGTACCGGTTGATGCCGGAAACCCTGGGCGTGGAGCGCCGTGACGGCTCGCGGCTGGCGTTCACGC‑CGATTCACctgct                                                                                                                                         <  2:76401/149‑1 (MQ=255)            tgcAATGGCTGGACTGGCGCTGGCTGTTCGTGGCATTCGCCATCGGCGCGGTGCTGACCTGGCTGCTGCTGTACCGGTTGATGCCGGAAACCCTGGGCGTGGAGCGCCGTGAGGGCTCGCGGCTGGCGTTCACGC‑TGATTCACctgctg                                                                                                                                        >  5:359291/1‑149 (MQ=255)            tgcAATGGCTGGACTGGCGCTGGCTGTTCGTGGCATTCGCCATCGGCGCGGTGCTGACCTGGCTGCTGCTGTACCGGTTGATGCCGGAAACCCTGGGCGTGGAGCGCCGTGACGGCTCGCGTCTGGCGTTCACGC‑TGATTCACctgctg                                                                                                                                        >  5:353909/1‑149 (MQ=255)            tgcAATGGCTGGACTGGCGCTGGCTGTTCGTGGCATTCGCCATCGGCGCGGTGCTGACCTGGCTGCTGCTGTACCGGTTGATGCCGGAAACCCTGGGCGTGGAGCGCCGTGACGGCTCGCGGCTGGCGTTCACGC‑CGATTCACctgctg                                                                                                                                        >  4:350139/1‑149 (MQ=255)                 tGGCTGGACTGGCGCTGGCTGTTCGTGGCATTCGCCATCGGCGCGGTGCTGACCTGGCTGCTGCTGTACCGGTTGATGCCGGAAACCCTGGGCGTGGAGCGCCGTGACGGCTCGCGGCTGGCGTTCACGC‑CGATTCACCTGCTGCCACt                                                                                                                                   <  1:65857/149‑1 (MQ=255)                    cTGGACTGGCGCTGGCTGTTCGTGGCATTCGCCATCGGCGCGGTGCTGACCTGGCTGCTGCTGTACCGGTTGATGCCGGAAACCCTGGGCGTGGAGCGCCGTGACGGCTCGCGGCTGGCGTTCACGC‑TGATTCACCTGCTGCCACTGtt                                                                                                                                <  6:359291/149‑1 (MQ=255)                    cTGGACTGGCGCTGGCTGTTCGTGGCATTCGCCATCGGCGCGGTGCTGACCTGGCTGCTGCTGTACCGGTTGATGCCGGAAACCCTGGGCGTGGAGCGCCGTGACGGCTCGCGGCTGGCGTTCACGC‑TGATTCACCTGCTGCCACTGtt                                                                                                                                <  6:353909/149‑1 (MQ=255)                        aCTGGCGCTGGCTGTTCGTGGCATTCGCCATCGGCGCGGTGCTGACCTGGCTGCTGCTGTACCGGTTGATGCCGGAAACCCTGGGCGTGGAGCGCCGTGACGGCTCGCGGCTGGCGTTCACGC‑CGATTCACCTGCTGCCACTGTTGGCg                                                                                                                            <  3:364964/149‑1 (MQ=255)                          tGGCGCTGGCTGTTCGTGGCATTCGCCATCGGCGCGGTGCTGACCTGGCTGCTGCTGTACCGGTTGATGCCGGAAACCCTGGGCGTGGAGCGCCGTGACGGCTCGCGGCTGGCGTTCACGC‑CGATTCACCTGCTGCCACTGTTGGCggg                                                                                                                          >  6:14849/1‑149 (MQ=255)                            gcgcTGGCTGTTCGTGGCATTCGCCATCGGCGCGGTGCTGACCTGGCTGCTGCTGTACCGGTTGATGCCGGAAACCCTGGGCGTGGAGCGCCGTGACGGCTCGCGGCTGGCGTTCACGC‑CGATTCCCCTGCTGCCACTGTTGGCGGGc                                                                                                                         >  2:94471/1‑148 (MQ=255)                              gctggctgTTCGTGGCATTCGCCATCGGCGCGGTGCTGACCTGGCTGCTGCTGTACCGGTTGATGCCGGAAACCCTGGGCGTGGAGCGCCGTGACGGCTCGCGGCTGGCGTTCACGC‑CGATTCACCTGCTGCCACTGTTGGCGGGCTAt                                                                                                                      >  7:316878/1‑149 (MQ=255)                              gctggctgTTCGTGGCATTCGCCATCGGCGCGGTGCTGACCTGGCTGCTGCTGTACCGGTTGATGCCGGAAACCCTGGGCGTGGAGCGCCGTGACGGCTCGCGGCTGGCGTTCACGC‑CGATTCACCTGCTGCCACTGTTGGCGGGCTAt                                                                                                                      <  6:150871/149‑1 (MQ=255)                               ctggctgTTCGTGGCATTCTCCATCGGCGCGGTTCTGACCTGGCTGCTTCTGTACCGGTTGATGCCGGAAACCCTGGGCGTGGAGCGCCGTGACGGCTCGCGGCTGGCGTTCACGC‑CGATTCACCTGCTGCCACTGTTGGCGGGCTATg                                                                                                                     <  6:228362/149‑1 (MQ=255)                               ctgccggTTCGTGGCATTCGCCATCGGCGCGGGGCGGCCCTGGCTGCTGCGGCCCCGGTTGCTGCCCGAAACCCTGGGCGTGGAGCGCCGTGACGGCTCGCGGCTGCCGTTCACGC‑CGATTCACCTGCTGCCACTGTTGGCGGGCTATg                                                                                                                     <  4:26227/143‑1 (MQ=255)                                  gctgTTCGTGGCATTCGCCATCGGCGCGGTGCTGACCTGGCTGCTGCTGTACCGGTTGATGCCGGAAACCCTGGGCGTGGAGCGCCGTGACGGCTCGCGGCTGGCGTTCACGC‑CGATTCACCTGCTGCCACTGTTGGCGGGCTATGGc                                                                                                                   >  7:104202/1‑148 (MQ=255)                                  gctgTTCGTGGCATTCGCCATCGGCGCGGTGCTGACCTGGCTGCTGCTGTACCGGTTGATGCCGGAAACCCTGGGCGTGGAGCGCCGTGACGGCTCGCGGCTGGCGTTCACGC‑CGATTCACCTGCTGCCACTGTTGGCGGGCTATGGc                                                                                                                   >  5:175025/1‑148 (MQ=255)                                    tgTTCGTGGCATTCGCCATCGGCGCGGTGCTGACCTGGCTGCTGCTGTACCGGTTGATGCCGGAAACCCTGGGCGTGGAGCGCCGTGACGGCTCGCGGCTGGCGTTCACGC‑CGATTCACCTGCTGCCACTGTTGGCGGGCTATGGCCAg                                                                                                                >  1:282368/1‑149 (MQ=255)                                    tgTTCGTGGCATTCGCCATCGGCGCGGTGCTGACCTGGCTGCTGCTGTACCGGTTGATGCCGGAAACCCTGGGCGTGGAGCGCCGTGACGGCTCGCGGCTGGCGTTCACGC‑CGATTCACCTGCTGCCACTGTTGGCGGGCTATGGCCAg                                                                                                                >  4:352517/1‑149 (MQ=255)                                        cGTGGCATTCGCCATCGGCGCGGTGCTGACCTGGCTGCTGCTGTACCGGTTGATGCCGGAAACCCTGGGCGTGGAGCGCCGTGACGGCTCGCGGCTGGCGTTCACGC‑CGATTCACCTGCTGCCACTGTTGGCg                                                                                                                            >  8:233525/1‑133 (MQ=255)                                        cGTGGCATTCGCCATCGGCGCGGTGCTGACCTGGCTGCTGCTGTACCGGTTGATGCCGGAAACCCTGGGCGTGGAGCGCCGTGACGGCTCGCGGCTGGCGTTCACGC‑CGATTCACCTGCTGCCACTGTTGGCg                                                                                                                            <  7:233525/133‑1 (MQ=255)                                         gTGGCATTCGCCATCGGCGCGGTGCTGACCTGGCTGCTGCTGTACCGGTTGATGCCGGAAACCCTGGGCGTGGAGCGCCGTGACGGCTCGCGGCTGGCGTTCACGC‑CGATTCACCTGCTGCCACTGTTGGCGGGCTATGGCCAGTTGCt                                                                                                           <  3:350139/149‑1 (MQ=255)                                           ggCATTCGCCATCGGCGCGGTGCTGACCTGGCTGCTGCTGTACCGGTTGATGCCGGAAACCCTGGGCGTGGAGCGCCGTGACGGCTCGCGGCTGGCGTTCACGC‑CGATTCACCTGCTGCCACTGTTGGCGGGCTATGGCCAGTTGCTgg                                                                                                         >  6:271721/1‑149 (MQ=255)                                                  gCCATCGGCGCGGTGCTGACCTGGCTGCTGCTGTACCGGTTGATGCCGGAAACCCTGGGCGTGGAGCGCCGTGACGGCTCGCGGCTGGCGTTCACGC‑CGATTCACCTGCTGCCACTGTTGGCGGGCTATGGCCAGTTGCTGGGCAAccg                                                                                                  >  6:203575/1‑149 (MQ=255)                                                    cATCGGCGCGGTGCTGACCTGGCTGCTGCTGTACCGGTTGATGCCGGAAACCCTGGGCGTGGAGCGCCGTGACGGCTCGCGGCTGGCGTTCACGC‑CGATTCACCTGCTGCCACTGTTGGCGGGc                                                                                                                         >  3:255757/1‑124 (MQ=255)                                                    cATCGGCGCGGTGCTGACCTGGCTGCTGCTGTACCGGTTGATGCCGGAAACCCTGGGCGTGGAGCGCCGTGACGGCTCGCGGCTGGCGTTCACGC‑CGATTCACCTGCTGCCACTGTTGGCGGGc                                                                                                                         <  4:255757/124‑1 (MQ=255)                                                    cATCGGCGCGGTGCTGACCTGGCTGCTGCTGTACCGGTTGATGCCGGAAACCCTGGGCGTGGAGCGCCGTGACGGCTCGCGGCTGGCGTTCACGC‑CGATTCACCTGCTGCCACTGTTGGCGGGCTATGGCCAGTTGCTGGGCAAccgcc                                                                                                <  8:104202/149‑1 (MQ=255)                                                        ggCGCGGTGCTGACCTGGCTGCTGCTGTACCGGTTGATGCCGGAAACCCTGGGCGTGGAGCGCCGTGACGGCTCGCGGCTGGCGTTCACGC‑CGATTCACCTGCTGCCACTGTTGGCGGGCTATGGCCAGTTGCTGGGCAACCGCCGCt                                                                                             <  6:133762/148‑1 (MQ=255)                                                         gcgcGGTGCTGACCTGGCTGCTGCTGTACCGGTTGATGCCGGAAACCCTGGGCGTGGAGCGCCCTGACGGCTCGCGGCTGGCGTTCACTC‑CGATTCACCTGATGCCACTGTAGGCGGGCTATGGCCAGATGCTTGGCAACCGCAGCt                                                                                             >  4:104444/1‑147 (MQ=255)                                                          cgcgGTGCTGACCTGGCTGCTGCTGTACCGGTTGATTCCGGAAACCCTGGGCGTGGAGCGCCGTGACGGCTCGCGGCTGGCGTTCACGC‑CGATTCACCTGCTGCCACTGTTGGCGGGCTATGGCCAGTTGCTGGGCAACCGCCGCTTCg                                                                                          <  8:128804/149‑1 (MQ=255)                                                             ggTGCTGACCTGGCTGCTGCTGTACCGGTTGATGCCGGAAACCCTGGGCGTGGAGCGCCGTGACGGCTCGCGGCTGGCGTTCACGC‑CGATTCACCTGCTGCCACTGTTGGCGGGCTATGGCCTGTTGCTGGGCAACCGCCGCTTCGTTg                                                                                       <  1:9007/149‑1 (MQ=255)                                                               tGCTGACCTGGCTGCTGCTGTACCGGTTGATGCCGGAAACCCTGGGCGTGGAGCGCCGTGACGGCTCGCGGCTGGCGTTCACGC‑CGATTCACCTGCTGCCACTGTTGGCGGGCTATGGCCAGTTGCTGGGCAACCGCCGCTTCGTTgca                                                                                     <  8:316878/149‑1 (MQ=255)                                                               tGCTGACCTGGCTGCTGCTGTACCGGTTGATGCCGGAAACCCTGGGCGTGGAGCGCCGTGACGGCTCGCGGCTGGCGTTCACGC‑CGATTCACCTGCTGCCACTGTTGGCGGGCTATGGCCAGTTGCTGGGCAACCGCCGCTTCGTTgca                                                                                     <  6:175025/149‑1 (MQ=255)                                                                      cTGGCTGCTGCTGTACCGGTTGATGCCGGAAACCCTGGGCGTGGAGCGCCGTGACGGCTCGCGGCTGGCGTTCACGC‑CGATTCACCTGCTGCCACTGTTGGCGGGCTATGGCCAGTTGCTGGGCAACCGCCGCTTCGTTGCAGGCAgcg                                                                              >  1:134827/1‑149 (MQ=255)                                                                             ctgctgTACCGGTTGATGCCGGAAACCCTGGGCGTGGAGCGCCGTGACGGCTCGCGGCTGGCGTTCACGC‑CGATTCAGCTGCTGCCACTGTTGTCGGGCTATGGCCAGTTGCTGGGCAACCGCCGCTTCGTTGCAGGCAGCGCCGCGCt                                                                       >  8:125503/1‑149 (MQ=255)                                                                              tgctgTACCGGTTGATGCCGGAAACCCTGGGCGTGGAGCGCCGTGACGGCTCGCGGCTGGCGTTCACGC‑CGATTCACCTGCTGCCACTGTTGGCGGGCTATGGCCAGTTGCTGGGCAACCGCCGCTTCGTTGCAGGCCGCGCCGCCCtt                                                                      >  4:295644/1‑149 (MQ=255)                                                                                ctgTACCGGTTGATGCCGGAAACCCTGGGCGTGGAGCGCCGTGACGGCTCGCGGCTGGCGTTCACGC‑CGATTCACCTGCTGCCACTGTTGGCGGGCTATGGCCAGTTGCTGGGCAACCGCCGCTTCGTTGCAGGCAGCGCCGCCCtt                                                                      <  5:271721/147‑1 (MQ=255)                                                                                   tACCGGTTGATGCCGGAAACCCTGGGCGTGGAGCGCCGTGACGGCTCGCGGCTGGCGTTCACGC‑CGATTCACCTGCTGCCACTGTTGGCGGGCTATGGCCAGTTGCTGGGCAACCGCCGCTTCGTTGCAGGCAGCGCCGCCCTTGGGtt                                                                 >  7:188703/1‑149 (MQ=255)                                                                                     ccGGTTGATGCCGGAAACCCTGGGCGTGGAGCGCCGTGACGGCTCGCGGCTGGCGTTCACGC‑CGATTCACCTGCTGCCACTGTTGGCGGGCTATGGCCAGTTGCTgg                                                                                                         >  1:232311/1‑107 (MQ=255)                                                                                     ccGGTTGATGCCGGAAACCCTGGGCGTGGAGCGCCGTGACGGCTCGCGGCTGGCGTTCACGC‑CGATTCACCTGCTGCCACTGTTGGCGGGCTATGGCCAGTTGCTgg                                                                                                         <  2:232311/107‑1 (MQ=255)                                                                                     ccGGTTGATGCCGGAAACCCTGGGCGTGCAGCGCCGTGACCGCTCGCGGCTGGCGTTCACGCATAATTAACATGCTTCCAATGTTGGCGGGCTATGGCCCGTTGATGGGCAACCGCCGCTTCTTTGCAGCACGCCCCGCCGTTGGGTTg                                                                >  1:302308/1‑149 (MQ=255)                                                                                        gTTGATGCCGGAAACCCTGGGCGTGGAGCGCCGTGACGGCTCGCGGCTGGCGTTCACGC‑CGATTCACCTGCTGCCACTGTTGGCGGGCTATGGCCAGTTGCTGGGCAACCGCCGCTTCGTTGCAGGCAGCGCCGCCCTTGGGTTGGTCg                                                            <  2:124014/149‑1 (MQ=255)                                                                                             tGCCGGAAACCCTGGGCGTGGAGCGCCGTGACGGCTCGCGGCTGGCGTTCACGC‑CGATTCACCTGCTGCCACTGTTGGCGGGCTATGGCCAGTTGCTGGGCAACCGCCGCTTCGTTGCAGGCAGCGCCGCCCTTGGGTTGGTCGGATTg                                                       <  2:134827/149‑1 (MQ=255)                                                                                               ccGGAAACCCTGGGCGTGGAGCGCCGTGACGGCTCGCGGCTGGCGTTCACGC‑CGATTCACCTGCTGCCACTGTTGGCGGGCTAt                                                                                                                      >  5:254575/1‑84 (MQ=255)                                                                                               ccGGAAACCCTGGGCGTGGAGCGCCGTGACGGCTCGCGGCTGGCGTTCACGC‑CGATTCACCTGCTGCCACTGTTGGCGGGCTAt                                                                                                                      <  6:254575/84‑1 (MQ=255)                                                                                                cGGAAACCCTGGGCGTGGAGCGCCGTGACGGCTCGCGGCTGGCGTTCACGC‑CGATTCACCTGCTGCCACTGTTGGCGGGCTATGGCCAGTTGCTGGGCAACCGCCGCTTCGTTGCAGGCAGCGCCGCCCTTGGGTTGGTCGGATTGCCg                                                    >  5:80638/1‑149 (MQ=255)                                                                                                       ccTGGGCGTGGAGCGCCGTGACGGCTCGCGGCTGGCGTTCACGC‑CGATTCACCTGCTGCCACTGTTGGCGGGCTATGGCCAGTTGCTGGGCAACCGCCGCTTCGTTGCAGGCAGCGCCGCCCTTGGGTTGGTCGGATTGCCGCTGATTg                                             >  7:80184/1‑149 (MQ=255)                                                                                                       ccTGGGCGTGGAGCGCCGTGACGGCTCGCGGCTGGCGTTCACGC‑CGATTCACCTGCTGCCACTGTTGGCGGGCTATGGCCAGTTGCTGGGCAACCGCCGCTTCGTTGCAGGCAGCGCCGCCCTTGGGTTGGTCGGATTGCCGCTGATTg                                             >  4:262691/1‑149 (MQ=255)                                                                                                       ccTGGGCGTGGAGCGCCGTGACGGCTCGCGGCTGGCGTTCACGC‑CGATTCACCTGCTGCCACTGTTGGCGGGCTATGGCCAGTTGCTGGGCAACCGCCGCTTCGTTGCAGGCAGCGCCGCCCTTGGGTTGGTCGGATTGCCGCTGATTg                                             >  3:172533/1‑149 (MQ=255)                                                                                                        cTGGGCGTGGAGCGCCGTGACGGCTCGCGGCTGGCGTTCACGC‑CGATTCACCTGCTGCCACTGTTGGCGGGCTATGGCCAGTTGCTGGGCAACCGCCGCTTCGTTGCAGGCAGCGCCGCCCTTGGGTTGGTCGGATTGCCGCTGATTg                                             >  3:1821/1‑148 (MQ=255)                                                                                                        cTGGGCGTGGAGCGCCGTGACGGCTCGCGGCTGGCGTTCACGC‑CGATTCACCTGCTGCCACTGTTGGCGGGCTATGGCCAGTTGCTGGGCAACCGCCGCTTCGTTGCAGGCAGCGCCGCCCTTGGGTTGGTCGGATTGCCGCTGATTg                                             <  4:1821/148‑1 (MQ=255)                                                                                                         tGGGCGTGGAGCGCCGTGACGGCTCGCGGCTGGCGTTCACGC‑CGATTCACCTGCTGCCACTGTTGGCGGGCTATGGCCAGTTGCTGGGCaa                                                                                                     <  8:339888/91‑1 (MQ=255)                                                                                                         tGGGCGTGGAGCGCCGTGACGGCTCGCGGCTGGCGTTCACGC‑CGATTCACCTGCTGCCACTGTTGGCGGGCTATGGCCAGTTGCTGGGCaa                                                                                                     >  7:339888/1‑91 (MQ=255)                                                                                                         tGGGCGTGGAGCGCCGTGACGGCTCGCGGCTGGCGTTCACGC‑CGATTCACCTGCTGCCACTGTTGGCGGGCTATGGCCAGTTGCTGGGCAACCGCCGCTTCGTTGCAGGCAGCGCCGCCCTTGGGTTGGTCGGATTGCCGCTGATTGGc                                           >  7:354581/1‑149 (MQ=255)                                                                                                           ggCGTGGAGCGCCGTGACGGCTCGCGGCTGGCGTTCACGC‑CGATTCACCTGCTGCCACTGTTGGCGGGCTATGGCCAGTTGCTGGGCAACCGCCGCTTCGTTGCAGGCAGCGCCGCCCTTGGGTTGGTCGGATTGCCGCTGATTGGCt                                          <  5:14849/148‑1 (MQ=255)                                                                                                            gCGTGGAGCGCCGTGACGGCTCGCGGCTGGCGTTCACGC‑CGATTCACCTGCTGCCACTGTTGGCGGGCTATGGCCAGTTGCTGGGCAACCGCCGCTTCGTTGCAGGCAGCGCCGCCCTTgg                                                                    <  2:325912/121‑1 (MQ=255)                                                                                                            gCGTGGAGCGCCGTGACGGCTCGCGGCTGGCGTTCACGC‑CGATTCACCTGCTGCCACTGTTGGCGGGCTATGGCCAGTTGCTGGGCAACCGCCGCTTCGTTGCAGGCAGCGCCGCCCTTgg                                                                    >  1:325912/1‑121 (MQ=255)                                                                                                                   gcgcCGTGACGGCTCGCGGCTGGCGTTCACGC‑CGATTCACCTGCTGCCACTGTTGGCGGGCTATGGCCAGTTGCTGGGCAACCGCCGCTTCGTTGCAGGCAGCGCCGCCCTTGGGTTGGTCGGATTGCCGCTGATTGGCTGGATTGGcc                                 >  2:154534/1‑149 (MQ=255)                                                                                                                      ccGTGACGGCTCGCGGCTGGCGTTCACGC‑CGATTCACCTGCTGCCACTGTTGGCGGGCTATGGCCAGTTGCTGGGCAACCGCCGCTTCGTTGCAGGCAGCGCCGCCCTTGGGTTGGTCGGATTGCCGCTGATTGGCTGGATTGGCCTGt                              >  4:319468/1‑149 (MQ=255)                                                                                                                       cGTGACGGCTCGCGGCTGGCGTTCACGC‑CGATTCACCTGCTGCCACTGTTGGCGGGCTATGGCCAGTTGCTGGGCAACCGCCGCTTCGTTGCAGGCAGCGCCGCCCTTGGGTTGGTCGGATTGCCGCTGATTGGCTGGATTGGCCTGTc                             >  5:76884/1‑149 (MQ=255)                                                                                                                       cGTGACGGCTCGCGGCTGGCGTTCACGC‑CGATTCACCTGCTGCCACTGTTGGCGGGCTATGGCCAGTTGCTGGGCAACCGCCGCTTCGTTGCAGGCAGCGCCGCCCTTGGGTTGGTCGGATTGCCGCTGATTGGCTGGATTGGCCTGTc                             <  8:188703/149‑1 (MQ=255)                                                                                                                             ggCTCGCGGCTGGCGTTCACGC‑CGATTCACCTGCTGCCACTGTTGGCGGGCTATGGCCAGTTGCTGGGCAACCGCCGCTTCGTTGCAGGCAGCGCCGCCCTTGGGTTGGTCGGATTGCCGCTGATTGGCTGTATTGGCCTGTCGCCGGt                       >  8:337804/1‑149 (MQ=255)                                                                                                                                tCGCGGCTGGCGTTCACGC‑CGATTCACCTGCTGCCACTGTTGGCGGGCTATGGCCAGTTGCTGGGCAACCGCCGCTTCGTTGCAGGCAGCGCCGCCCTTGGGTTGGTCGGATTGCCGCTGATTGGCTGGATTGGCCTGTCGCCGGtgct                    >  7:159446/1‑149 (MQ=255)                                                                                                                                tCGCGGCTGGCGTTCACGC‑CGATTCACCTGCTGCCACTGTTGGCGGGCTATGGCCAGTTGCTGGGCAACCGCCGCTTCGTTGCAGGCAGCGCCGCCCTTGGGTTGGTCGGATTGCCGCTGATTGGCTGGATTGGCCTGTCGCCGGtgct                    >  5:104731/1‑149 (MQ=255)                                                                                                                                  gcgGCTGGCGTTCACGC‑CGATTCACCTGCTGCCACTGTTGGCGGGCTATGGCCAGTTGCTGGGCAACCGCCGCTTCGTTGCAGGCAGCGCCGCCCTTGGGTTGGTCGGATTGCCGCTGATTGGCTGGATTGGCCTGTCGCCGGtgctgc                  <  4:172533/149‑1 (MQ=255)                                                                                                                                  gcgGCTGGCGTTCACGC‑CGATTCACCTGCTGCCACTGTTGGCGGGCTATGGCCAGTTGCTGGGCAACCGCCGCTTCGTTGCAGGCAGCGCCGCCCTTGGGTTGGTCGGATTGCCGCTAATTGGCTGGATTGGCCTGTCGCCGGtgctgc                  <  2:282368/149‑1 (MQ=255)                                                                                                                                    ggCTGGCGTTCACGC‑CGATTCACCTGCTGCCACTGTTGGCGGGCTATGGCCAGTTGCTGGGCAACCGCCGCTTCGTTGCAGGCAGCGCCGCCCTTGGGTTGGTCGGATTGCCGCTGATTGGCTGGATTGGCCTGTCGCCGGTgctgct                 >  7:211737/1‑148 (MQ=255)                                                                                                                                         gCGTTCACGC‑CGATTCACCTGCTGCCACTGTTGGCGGGCTATGGCCAGTTGCTGGGCAACCGCCGCTTCGTTGCAGGCAGCGCCGCCCTTGGGTTGGTCGGATTGCCGCTGATTGGCTGGATTGGCCTGTCGCCGGTGCTGCTGATTCa           >  7:319720/1‑149 (MQ=255)                                                                                                                                           gTTCACGC‑CGATTCACCTGCTGCCACTGTTGGCGGGCTATGGCCAGTTGCTGGGCAACCGCCGCTTCGTTGCAGGCAGCGCCGCCCTTGGGTTGGTCGGATTGCCGCTGATTGGCTGGATTGGCCTGTCGCCGGTGCTGCTGATTCACg         <  8:82008/149‑1 (MQ=255)                                                                                                                                              atttc‑cGATTCACCTGCTGCCACTGTTGGCGGGCTATGGCCAGTTGCTGGGCAACCGCCGCTTCGTTGCAGGCAGCGCCGCCCTTGGGTTGGTCGGATTGCCGCTGATTGGCTGGATTGGCCTGTCGCCGGTGCTGCTGATTCACGAt       <  1:137041/144‑1 (MQ=255)                                                                                                                                               aCGC‑CGATTCACCTGCTGCCACTGTTGGCGGGCTATGGCCAGTTGCTGGGCAACCGCCGCTTCGTTGCAGGCAGCGCCGCCCTTGGGTTGGTCGGATTGCCGCTGATTGGCTGGATTGGCCTGTCGCCGGTGCTGCTGATTCACGAt       >  7:296914/1‑147 (MQ=255)                                                                                                                                                cGC‑CGATTCACCTGCTGCCACTGTTGGCGGGCTATGGCCAGTTGCTGGGCAACCGCCGCTTCGTTGCAGGCAGCGCCGCCCTTGGGTTGGTCGGATTGCCGCTGATTGGCTGGATTGGCCTGTCGCCGGTGCTGCTGATTCACGATGAg    >  8:221245/1‑149 (MQ=255)                                                                                                                                                cGC‑CGATTCACCTGCTGCCACTGTTGGCGGGCTATGGCCAGTTGCTGGGCAACCGCCGCTTCGTTGCAGGCAGCGCCGCCCTTGGGTTGGTCGGATTGCCGCTGATTGGCTGGATTGGCCTGTCGCCGGTGCTGCTGATTCACGATGAg    <  7:221245/149‑1 (MQ=255)                                                                                                                                                 gC‑CGATTCACCTGCTGCCACTGTTGGCGGGCTATGGCCAGTTGCTGGGCAACCGCCGCTTCGTTGCAGGCAGCGCCGCCCTTGGGTTGGTTGGATTGCCGCTGATTGGCTGGATTGGCCTGTCGCCGGTGCTGCTGATTCACGATGAgg   >  8:176720/1‑149 (MQ=255)                                                                                                                                                 gC‑CGATTCACCTGCTGCCACTGTTGGCGGGCTATGGCCAGTTGCTGGGCAACCGCCGCTTCGTTGCAGGCAGCGCCGCCCTTGGGTTGGTCGGATTGCCGCTGATTGGCTGGATTGGCCTGTCGCCGGTGCTGCTGATTCACGATGAgg   <  8:319720/149‑1 (MQ=255)                                                                                                                                                 gC‑CGATTCACCTGCTGCCACTGTTGGCGGGCTATGGCCAGTTGCTGGGCAACCGCCGCTTCGTTGCAGGCAGCGCCGCCCTTGGGTTGGTCGGATTGCCGCTGATTGGCTGGATTGGCCTGTCGCCGGTGCTGCTGATTCACGATGAgg   <  8:354581/149‑1 (MQ=255)                                                                                                                                                  gacGATTCACCTGCTGCCACTGTTGGCGGGCTATGGCCAGTTGCTGGGCAACCGCCGCTTCGTTGCAGGCAGCGCCGCCCTTGGGTTGGTCGGATTGCCGCTGATTGGCTGGATTGGCCTGTCGCCGGTGCTGCTGATTCACGATGAgg   <  3:277015/147‑1 (MQ=255)                                                                                                                                                  c‑cGATTCACCTGCTGCCACTGTTGGCGGGCTATGGCCAGTTGCTGGGCAACCGCCGCTTCGTTGCAGGCAGCGCCGCCCTTGGGTTGGTCGGATTGCCGCTGATTGGCTGGATTGGCCTGTCGCCGGTGCTGCTGATTCACGATGAggg  <  3:352517/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                    CACCTTGCTGCTGCAATGGCTGGACTGGCGCTGGCTGTTCGTGGCATTCGCCATCGGCGCGGTGCTGACCTGGCTGCTGCTGTACCGGTTGATGCCGGAAACCCTGGGCGTGGAGCGCCGTGACGGCTCGCGGCTGGCGTTCACGC‑CGATTCACCTGCTGCCACTGTTGGCGGGCTATGGCCAGTTGCTGGGCAACCGCCGCTTCGTTGCAGGCAGCGCCGCCCTTGGGTTGGTCGGATTGCCGCTGATTGGCTGGATTGGCCTGTCGCCGGTGCTGCTGATTCACGATGAGGG  >  NC_002947/1543670‑1543963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑