Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,576,756 G→C 10.4% intergenic (‑276/+37) galP‑II ← / ← ttgC porin‑like protein/efflux pump outer membrane protein TtgC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,576,7560GC10.4% 157.4 / 7.9 56intergenic (‑276/+37)galP‑II/ttgCporin‑like protein/efflux pump outer membrane protein TtgC
Reads supporting (aligned to +/- strand):  ref base G (27/23);  new base C (4/2);  total (31/25)
Fisher's exact test for biased strand distribution p-value = 6.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.62e-01

CGCAAACGGAACGGGGCGATAATCGAACGGAATGTTCCGGTTGGTTAATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGCCACCGCCAAGCGCCTTGTACAGGTTGACCTCGCTGGTC  >  NC_002947/1576618‑1576892
                                                                                                                                          |                                                                                                                                        
cgcAAACGGAACGGGGCGATAATCGAACGGAATGTTCCGGTTGGTTAATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAAACCGCTCCTACGACGGCTTGCGCCCAGCAGGCAAAGGCAGTAAAAACAAAGCCCACCGATAAGGGTgg                                                                                                                                >  5:5094/1‑149 (MQ=255)
cgcAAACGGAACGGGGCGATAATCGAACGGAATGTTCCGGTTGGTTAATAACTCTTCTGGGGCGGCCTCTTCGCGGGGAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTgg                                                                                                                                >  5:282146/1‑149 (MQ=255)
 gcAAACGGAACGGGGCGATAATCGAACGGAATGTTCCGGTTGGTTAATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTggg                                                                                                                               >  7:47326/1‑149 (MQ=255)
 gcAAACGGAACGGGGCGATAATCGAACGGAATGTTCCGGTTGGTTAATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTggg                                                                                                                               <  6:203335/149‑1 (MQ=255)
 gcAAACGGAACGGGGCGATAATCGAACGGAATGTTCCGGTTGGTTAATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTggg                                                                                                                               >  6:222459/1‑149 (MQ=255)
   aaaCGGAACGGGGCGATAATCGAACGGAATGTTCCGGTTGGTTAATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCt                                                                                                                             <  3:215351/149‑1 (MQ=255)
     acggaacggGGCGATAATCGAACGGAATGTTCCGGTTGGTTAATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCCTTATCGGTGGGCttt                                                                                                                           <  3:326636/149‑1 (MQ=255)
      cggaacggGGCGATAATCGAACGGAATGTTCCGGTTGGTTAATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCttt                                                                                                                           <  4:157746/148‑1 (MQ=255)
      cggaacggGGCGATAATCGAACGGAATGTTCCGGTTGGTTAATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCttt                                                                                                                           >  3:157746/1‑148 (MQ=255)
        caacggGCCGATAATCGAACGCAATGTTCCGGTTGGTTAATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCCTTATCGGTGGGCtttgtt                                                                                                                        <  4:63455/148‑4 (MQ=38)
                        gtaCGGAATGTTCCGGTTGGTTAATATCTCTTCTGGGGCGGCCTCTTCGCGTGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGTGCTTTCTGCtttt                                                                                                                   <  2:326826/136‑1 (MQ=255)
                        gAACGGAATGTTCCGGTTGGTTAATAACTCTTCTGGGGCGGACTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGAACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGcc                                                                                                        >  3:362226/1‑149 (MQ=255)
                        gAACGGAATCTTCCGGTTGGTTAATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCATCAGGCACATGCAGTAAAAACAAAGCCCACAGATAAGGGTGGGAATTCTGAtttt                                                                                                                   >  1:326826/1‑138 (MQ=37)
                         aaCGGAATGTTCCGGTTGGTTAATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACAGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGcc                                                                                                        >  2:341840/1‑148 (MQ=255)
                          aCGGAATGTTCCGGTTGGTTAATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGa                                                                                                      >  5:195203/1‑149 (MQ=255)
                                tGTTCCGGTTGGTTAATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGcc                                                                                                >  8:312808/1‑149 (MQ=255)
                                       gttggttAATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGgt                                                                                         <  1:139101/149‑1 (MQ=255)
                                           gttAATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGttt                                                                                     <  5:326868/149‑1 (MQ=255)
                                           gttAATAACTCTTCTGGGGCGGAATATTCGATGGAAAACCCGCTCCTACGACGGCTTGCGCACAGACGTCACAGGCAGTAAAAACAAAGCCCACCGATAACGGTGGGCTTTCTTCTTTTGCTACAAGGAAGATCAGCCTTTCGTTGttt                                                                                     >  8:300374/1‑149 (MQ=255)
                                            ttAATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTc                                                                                    <  6:38928/149‑1 (MQ=255)
                                            ttAATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGACCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTc                                                                                    >  3:69575/1‑149 (MQ=255)
                                              aataaCTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCa                                                                                  >  8:178653/1‑149 (MQ=255)
                                              aataaCTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCa                                                                                  >  5:295763/1‑149 (MQ=255)
                                                   ctctTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGAt                                                                             <  4:180538/149‑1 (MQ=255)
                                                           gggCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCctgctg                                                                     >  2:230341/1‑149 (MQ=255)
                                                               ggcCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTg                                                                 <  6:142490/149‑1 (MQ=255)
                                                               ggcCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTg                                                                 <  4:258937/149‑1 (MQ=255)
                                                                 ccTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGc                                                               <  4:175269/149‑1 (MQ=255)
                                                                  ctctTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTgg                                                                    >  2:319676/1‑143 (MQ=255)
                                                                     ttCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCt                                                             <  6:195203/147‑1 (MQ=255)
                                                                          gggTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGt                                                      <  7:312808/149‑1 (MQ=255)
                                                                          gggTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGt                                                      <  5:222459/149‑1 (MQ=255)
                                                                               aagccGCTCCTACGACGGCTTTCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTTGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCTGTCTGc                                                  <  6:5094/145‑1 (MQ=255)
                                                                               aaCCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGc                                                  <  1:230341/148‑1 (MQ=255)
                                                                                 cccGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCg                                               >  7:118345/1‑149 (MQ=255)
                                                                                  ccGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCg                                               >  4:21773/1‑148 (MQ=255)
                                                                                    gCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTAc                                            >  5:79052/1‑149 (MQ=255)
                                                                                        cTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGc                                        <  6:334678/149‑1 (MQ=255)
                                                                                         tACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGcc                                       >  4:19934/1‑149 (MQ=255)
                                                                                          acgacgGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGCCa                                      >  5:97523/1‑149 (MQ=255)
                                                                                           cgacgGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGACCGGTCTGCTCGTACCAGCAAc                                     >  2:243074/1‑149 (MQ=255)
                                                                                                     cgcCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGCCACCGCCAAgcg                            <  8:47326/148‑1 (MQ=255)
                                                                                                        ccAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTATCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGCCACCGCCAAGCGCCtt                        <  6:282146/149‑1 (MQ=255)
                                                                                                            ccGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGCCACCGCCAAGCGCCTTGTa                     >  7:196619/1‑148 (MQ=255)
                                                                                                                cacaGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGCCACCGCCAAGCGCCTTGTACAGGt                <  4:69575/149‑1 (MQ=255)
                                                                                                                     gCAGTAAAAACAAAGCCGACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGCCACCGCCAAGCGCCTTGTACAGGTTGAcc           <  2:72635/149‑1 (MQ=255)
                                                                                                                       aGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGCCACCGCCAAGCGCCTTGTACAGGTTGAccag         >  7:10755/1‑147 (MQ=255)
                                                                                                                         tAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGt                                              <  7:346931/110‑1 (MQ=255)
                                                                                                                         tAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGt                                              >  8:346931/1‑110 (MQ=255)
                                                                                                                         tAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTATCCACCGATGCCTGCTGGTAGGCCTGCCCGGTCTGCTCGTACCAGCCACCGCCAAGCGCCTTGTACAGGTTGACATCGc       >  5:6401/1‑149 (MQ=255)
                                                                                                                          aaaaaCAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGCCACCGCCAAGCGCCTTGTACAGGTTGACCTCGCt      >  2:215659/1‑149 (MQ=255)
                                                                                                                            gcagaaaGCCCACCCTTATCGGTGGGCTTTCTGCTTTTGCAACAAGGCAGAACAGACTTTCGGTGTTACCACCGATGCCTGCTCGTTGGCCTGCCCGGTCTGCTCGTACCAGCCACCGCCAAGCGCCTTGTACAGGTTGACCTCGCTg     >  2:164917/5‑148 (MQ=255)
                                                                                                                              agaaaGCCCACCCTTATCGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGCCACCGCCAAGCGCCTTGTACAGGTTGACCTCGCTGGt   >  5:18231/3‑148 (MQ=255)
                                                                                                                              agaaaGCCCACCCTTATCGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGCCACCGCCAAGCGCCTTGTACAGGTTGACCTCGCTGGt   >  7:179037/3‑148 (MQ=255)
                                                                                                                              aCAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGCCACCGCCAAGCGCCTTGTACAGGTTGACCTCGCTGGTc  <  1:152969/149‑1 (MQ=255)
                                                                                                                               gaaaGCCCACCCTTATCGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGCCACCGCCAAGCGCCTTGTACAGGTTGACCTCGCTGGt   >  4:248063/2‑147 (MQ=255)
                                                                                                                                          |                                                                                                                                        
CGCAAACGGAACGGGGCGATAATCGAACGGAATGTTCCGGTTGGTTAATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAGGCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGCCACCGCCAAGCGCCTTGTACAGGTTGACCTCGCTGGTC  >  NC_002947/1576618‑1576892

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑