Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,581,266 G→T 100% A44D (GCC→GAC)  ttgB ← efflux pump membrane protein TtgB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,581,2660GT96.8% 200.5 / ‑1.5 62A44D (GCC→GAC) ttgBefflux pump membrane protein TtgB
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (30/30);  total (31/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.33e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GCGGTAATGGTCATGCTGCCGTCGGAGTTACTTTCCGACGACACATAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGGCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCACCCAGGCGAAGATCGGGCGATCGATAAAGAACTTCGACATGGTT  >  NC_002947/1581122‑1581400
                                                                                                                                                |                                                                                                                                      
gCGGTAATGGTCATGCTGCCGTCGGAGTTACTTTCCGACGACACATAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACggcgatgtcgat                                                                                                                                    >  3:28642/1‑149 (MQ=255)
gCGGTAATGGTCATGCTGCCGTCGGAGTTACTTTCCGACGACACATAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACggcgatgtcga                                                                                                                                     <  8:171440/148‑1 (MQ=255)
 aggTAATGGTCATGCGGCCGTCGGAGTTACTTTCCGACGACACATAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGgcgatgtcgatg                                                                                                                                   <  7:342661/148‑1 (MQ=255)
   gTAATGGTCATGCTGCCGTCGGAGTTACTTTCCGACGACACATAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCgatgtcgatggc                                                                                                                                 >  7:203393/1‑149 (MQ=255)
    tAATGGTCATGCTGCCGTCGGAGTTACTTTCCGACGACACATAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGcgatgtcgatggat                                                                                                                                >  6:208844/1‑147 (MQ=255)
     aaTGGTCATGCTGCCGTCGGAGTTACTTTCCGACGACACATAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGAtggctg                                                                                                                               >  2:336010/1‑149 (MQ=255)
                   cGTCGGAGTTACTTTCCGACGACACATAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTg                                                                                                                 <  5:370148/149‑1 (MQ=255)
                    gTCGGAGTTACTTTCCGACGACACATAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTgg                                                                                                                >  2:362226/1‑149 (MQ=255)
                     tCGGAGTTACTTTCCGACGACACATAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACggcgatgtcgat                                                                                                                                    >  6:90834/1‑128 (MQ=255)
                     tCGGAGTTACTTTCCGACGACACATAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACggcgatgtcgat                                                                                                                                    <  5:90834/128‑1 (MQ=255)
                     tCGGAGTTACTTTCCGACGACACATAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCCGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTggg                                                                                                               >  5:163047/1‑149 (MQ=255)
                              cTTTCCGACGACACATAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGt                                                                                                       <  3:1596/148‑1 (MQ=255)
                               tttCCGACGACACATAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGtt                                                                                                      >  6:159645/1‑148 (MQ=255)
                               tttCCGACGACACATAACCCAGGTTGTCGATACCGTTGAGCTGCTCCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGAAGTCGATGGCTGGCGGCGCGATACTGCGGTACTGGtt                                                                                                      >  8:263382/1‑148 (MQ=255)
                                   cgacgaCACATAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATc                                                                                                  <  5:350041/148‑1 (MQ=255)
                                    gacgacACATAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATc                                                                                                  <  7:10463/147‑1 (MQ=255)
                                    gacgacACATAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATc                                                                                                  >  8:10463/1‑147 (MQ=255)
                                     acgacAGATTACGCAGGTTGTCGATAGCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCTAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGc                                                                                               <  4:204672/149‑1 (MQ=255)
                                         cacaTAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGCTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTTGAGGCGCGATACAGGGGTACTGGTTGATCGGCAGCt                                                                                           >  3:3723/1‑149 (MQ=255)
                                          acaTAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCtt                                                                                          >  2:105869/1‑149 (MQ=255)
                                            aTAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCa                                                                                        <  8:176802/149‑1 (MQ=255)
                                             tAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAg                                                                                       <  2:4065/149‑1 (MQ=255)
                                                cGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGAt                                                                                    <  4:28642/149‑1 (MQ=255)
                                                 gCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATg                                                                                   <  2:139345/149‑1 (MQ=255)
                                                   aGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCCATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGa                                                                                 <  3:8043/149‑1 (MQ=255)
                                                     gTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACggcgatgtcgat                                                                                                                                    >  3:90705/1‑96 (MQ=255)
                                                     gTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACggcgatgtcgat                                                                                                                                    <  4:90705/96‑1 (MQ=255)
                                                             tACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCg                                                                       <  5:194719/149‑1 (MQ=255)
                                                                cGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTCCTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGAcc                                                                    >  7:346881/1‑149 (MQ=255)
                                                                cGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGAc                                                                     >  6:40275/1‑148 (MQ=255)
                                                                    gAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGc                                                                 <  1:336010/148‑1 (MQ=255)
                                                                      gctgctgctCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCTATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCAt                                                               >  8:177621/1‑148 (MQ=255)
                                                                        tgctgctCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGAt                                                            >  3:153120/1‑149 (MQ=255)
                                                                          ctgctCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATc                                                           <  5:159645/148‑1 (MQ=255)
                                                                          ctgctCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATc                                                           <  5:40275/148‑1 (MQ=255)
                                                                          ctgctCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCa                                                          >  3:40604/1‑149 (MQ=255)
                                                                          ctgctCGATCACCTGCACCACGGTGTCCTGCACGGCTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGAt                                                            <  8:107774/147‑1 (MQ=255)
                                                                            gctCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCAc                                                         >  2:227577/1‑148 (MQ=255)
                                                                            gctCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCAc                                                         >  6:181960/1‑148 (MQ=255)
                                                                              tCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGGCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCAcc                                                        >  5:153037/1‑147 (MQ=255)
                                                                                 aTCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGc                                                   <  5:107110/149‑1 (MQ=255)
                                                                                     ccTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCTGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATc                                               <  4:40604/149‑1 (MQ=255)
                                                                                      cTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCa                                              >  1:364400/1‑149 (MQ=255)
                                                                                        gCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCAcc                                            >  5:138233/1‑149 (MQ=255)
                                                                                          accacGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCACCCa                                          <  8:203393/149‑1 (MQ=255)
                                                                                            cacGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCACCCAgg                                        >  5:376199/1‑149 (MQ=255)
                                                                                            cacGGTGTCCTGCACGGTTTGCGCCAAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCa                                                                >  7:118706/1‑125 (MQ=255)
                                                                                            cacGGTGTCCTGCACGGTTTGCGCCAAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCa                                                                <  8:118706/125‑1 (MQ=255)
                                                                                             acGGTGTGCTTCCAGGGTTTCGCCGAGGGGGCCGGGTATGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCACCCAGGc                                       <  5:208844/149‑1 (MQ=255)
                                                                                             acGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCACCCAGGc                                       >  5:829/1‑149 (MQ=255)
                                                                                              cGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCACCCAGGc                                       >  6:234283/1‑148 (MQ=255)
                                                                                                gtgtCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCACCCAGGCGaa                                    <  6:138233/149‑1 (MQ=255)
                                                                                                        cACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCACCCAGGCGAAGATTggg                             <  6:163047/148‑1 (MQ=255)
                                                                                                        cACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCACCCAGGCGAAGATCGGGc                            >  1:182855/1‑149 (MQ=255)
                                                                                                             tttGCGCCTAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCACCCAGGCGAAGATCGGGcgatcg                       <  7:177621/149‑1 (MQ=255)
                                                                                                             tttGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCACCCAGGCGAAGATCGGGcgatc                        >  1:106863/1‑148 (MQ=255)
                                                                                                                gcgcCGAGGCGCCCGGGTAGGTCACGGCGATGGCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCACCCAGGCGAAGATCGGGcgatcgat                     <  6:153037/148‑1 (MQ=255)
                                                                                                                  gcCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGCCCAGCATGATCACCAAGGCGATCACCCAGGCGAAGATCGGGCGATCGATa                    >  5:121833/1‑147 (MQ=255)
                                                                                                                  gcCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCACCCAGGCGAAGATCGGGc                            <  4:82144/139‑1 (MQ=255)
                                                                                                                  gcCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCACCCAGGCGAAGATCGGGc                            >  3:82144/1‑139 (MQ=255)
                                                                                                                  gcCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCACCCAGGCGAAGATCGGGCGATCGATaaa                  <  4:153120/149‑1 (MQ=255)
                                                                                                                   ccGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATAGGGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCACCCAGGCGAAGATCGGGCGATCGATa                    >  1:283115/1‑146 (MQ=255)
                                                                                                                     gAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCACCCAGGCGAAGATCGGGCGATCGATAAAGaa               <  3:73662/149‑1 (MQ=255)
                                                                                                                        gcgcCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCACCCAGGCGAAGATCGGGCGATCGATAAAGAACtt            <  2:182855/149‑1 (MQ=255)
                                                                                                                         cggcctggTAGTTCACGTCGATGTCGATGGCTGGCGGCTCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGGCCCGACCAGCATGATCACCAAGGCGATCACCCAGGCGAAGATCGGGCGATCGATAAAGAACTTc           <  8:324506/143‑1 (MQ=255)
                                                                                                                          gcCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGGGCGATACTGGGGTACTGGTTGCTCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCACCCAGGCGAAGATCGGGCGATCGATAAAGAACTTc           >  5:17405/1‑148 (MQ=255)
                                                                                                                          gcCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGTTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGAACACCAAGGCCATCACCCAGGCGAAGATCGGGCGATCCATAAAGAACTTCg          >  8:331925/1‑149 (MQ=255)
                                                                                                                          gcCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCACCCAGGCGAAGATCGGGCGATCGATAAAGAACTTc           >  6:102646/1‑148 (MQ=255)
                                                                                                                              gggTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGa                                                                      >  5:226497/1‑85 (MQ=255)
                                                                                                                              gggTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGa                                                                      <  6:226497/85‑1 (MQ=255)
                                                                                                                                   ggTCACGGCGATGTAGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCACCCAGGCGAAGATCGGGCGATCGATAAAGAACTTCGACATGGtt  <  7:263382/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                      
GCGGTAATGGTCATGCTGCCGTCGGAGTTACTTTCCGACGACACATAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGGCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCACCCAGGCGAAGATCGGGCGATCGATAAAGAACTTCGACATGGTT  >  NC_002947/1581122‑1581400

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑