Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,622,907	C→G	6.6%	G150G (GGG→GGC)	PP_1422 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,622,907	0	C	G	6.6%	185.1 / 3.8	61	G150G (GGG→GGC)	PP_1422	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (29/28);  new base G (2/2);  total (31/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.18e-01

AACTCCCAGGCCTTGAGGATCTTGTCCCCCAGCACCGGCTGGAGTTGCTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCGGCGAGCGGGCATGGAAAATCTGCTCGATCA  >  NC_002947/1622764‑1623055                                                                                                                                                |                                                                                                                                                     aaCTCCCAGGCCTTGAGGATCTTGTCCCCCAGCACCGGCTGGAGTTGCTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCGTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCATcccacc                                                                                                                                                 >  8:264798/1‑149 (MQ=255)      ccAGGCCTTGAGGATCTTGTCCCCCAGCACCGGCTGGAGTTGCTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGGATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAgg                                                                                                                                            >  7:204268/1‑149 (MQ=255)      ccAGGCCTTGAGGATCTTGTCCCCCAGCACCGGCTGGAGTTGCTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAg                                                                                                                                             <  8:129712/148‑1 (MQ=255)       cAGGCCTTGAGGATCTTGTCCCCCAGCACCGGCTGGAGTTGCTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGt                                                                                                                                           >  2:54729/1‑149 (MQ=255)               gAGGATCTTGTCCCCCAGCACCGGCTGGAGTTGCTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGGCCACCTTGGGTGGGCTgg                                                                                                                                    <  4:370461/148‑1 (MQ=255)                 ggAACTTGTCCCCCAGCAGCGGCTGGAGTTGCTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGATCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCAAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCg                                                                                                                                 >  6:290906/1‑149 (MQ=255)                  gATCTTGTCCCCCAGCACCGGCTGGAGTTGCTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCTGCAGCGCGCCAATCTGGTTGACCAGcccaccc                                                                                                                                                <  4:304105/133‑1 (MQ=255)                  gATCTTGTCCCCCAGCACCGGCTGGAGTTGCTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCAGCAGCGCGCCAATCTGGTTGACCAGcccaccc                                                                                                                                                >  3:304105/1‑133 (MQ=255)                        gTCCCCCAGCACCGGCTGGAGTTGCTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGGCCACCTTGGGTGGGCTGGTcaac                                                                                                                               <  7:50037/144‑4 (MQ=255)                         tCCCCCAGCACCGGCTGGAGTTGCTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTgag                                                                                                                         <  1:76930/149‑1 (MQ=255)                            cccAGCACCGGCTGGAGTTGCTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAgg                                                                                                                      >  2:93503/1‑149 (MQ=255)                             ccAGCACCGGCTGGAGTTGCTCGAGCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGgt                                                                                                                     <  6:263118/149‑1 (MQ=255)                              cAGCACCGGCTGGAGTTGCTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGgtg                                                                                                                    >  1:28513/1‑149 (MQ=255)                                gCACCGGCTGGAGTTGCTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGgtgt                                                                                                                   >  1:5359/1‑148 (MQ=255)                                       cTGGAGTTGCTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGACGGGTTTGAGAGGTGTGAAGCGAc                                                                                                           <  5:143984/149‑1 (MQ=255)                                        gggAGTTGCTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGTCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCATCCCACCCAAGGTGGGCTGGTCGGGTTTGAGAGGTGTGAAGCGACg                                                                                                          >  5:267326/2‑149 (MQ=255)                                          gAGTTGCTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCgg                                                                                                                                >  5:18067/1‑125 (MQ=255)                                          gAGTTGCTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCgg                                                                                                                                <  6:18067/125‑1 (MQ=255)                                          gAGTTGCTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAACGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACgg                                                                                                         >  7:229158/1‑148 (MQ=255)                                           aGTTGCTCGATCACATAATGCAGGCAAACCGGGTCGGACAAGAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCa                                                                                                       <  5:324169/149‑1 (MQ=255)                                             ttGCTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACgg                                                                                                         >  5:313901/1‑145 (MQ=255)                                               gCTCGATCACATAATGCAGGCAAACCGACTAGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGCAAGCGCGCAAATCTGGTTGACCACCAAACCCAACGTCGCCTGGTCCGGTTTCAGAGGTGTGAAGAGACGGCAGAt                                                                                                    >  2:17144/1‑148 (MQ=255)                                                cTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCTGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAATGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGAttt                                                                                                  <  5:23565/149‑1 (MQ=255)                                                    aTCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTa                                                                                              <  1:54729/149‑1 (MQ=255)                                                    aTCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTa                                                                                              <  2:28513/149‑1 (MQ=255)                                                    aTCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTa                                                                                              <  1:93503/149‑1 (MQ=255)                                                       acaTAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGc                                                                                            <  6:219422/148‑1 (MQ=255)                                                          tAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGAt                                                                                        >  7:145632/1‑149 (MQ=255)                                                            aTGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATg                                                                                       <  3:326421/148‑1 (MQ=255)                                                              gCAGGCAAACCGGGTCGGAAAACAGTTCGTAGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGATTGAGAGGTGTGAACCGACGGCAGAATTCCGAGCTGATGccc                                                                                    >  2:139949/1‑149 (MQ=255)                                                               cAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGccc                                                                                    <  1:71064/148‑1 (MQ=255)                                                               cAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCg                                                                                   >  4:356664/1‑149 (MQ=255)                                                                 ggCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGcc                                                                                 <  6:313901/149‑1 (MQ=255)                                                                 ggCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGcc                                                                                 <  3:108621/149‑1 (MQ=255)                                                                        cGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCa                                                                          <  7:85720/149‑1 (MQ=255)                                                                               gACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGAGCCAATCTGGTTGACCAGCCCAACCACGGTGGCCTGGTAGGGGTTGAGAGTTGTGAAGCGCCCGCAGAATTCGTAGCTGATGCCCGCCACTTCCAGTCTaat                                                                   >  5:57213/1‑147 (MQ=255)                                                                                      gTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGcc                                                               >  5:63889/1‑146 (MQ=255)                                                                                          gTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGatat                                                        >  8:349111/1‑149 (MQ=255)                                                                                             gtgCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCAGCCATGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATct                                                      <  1:139949/148‑2 (MQ=255)                                                                                               gCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATcgcg                                                    >  1:53995/1‑148 (MQ=255)                                                                                                        cATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCCCCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTATCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCCCAGTTTCTGc                                          >  6:296198/1‑149 (MQ=255)                                                                                                        cATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGc                                          >  6:295239/1‑149 (MQ=255)                                                                                                             aTGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTATCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTATGCTCCa                                      >  8:276383/1‑148 (MQ=255)                                                                                                               gAGTACCGGAAGCGCGCCAATCTGGTTGAGCAGCCCACCCAAGGTGGCCTGGTCGTGTTTGAGAGGTGTGAAGCGACTGCAGATTTCGTAGCTGATGCCCGCCCCTTCCAGTCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACt                                    <  1:78759/148‑1 (MQ=255)                                                                                                                  tACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCg                                >  5:56458/1‑149 (MQ=255)                                                                                                                    ccGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCGGc                              >  5:270487/1‑149 (MQ=255)                                                                                                                     cGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGccc                                                              >  7:24461/1‑116 (MQ=255)                                                                                                                     cGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGccc                                                              <  8:24461/116‑1 (MQ=255)                                                                                                                     cGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCGGc                              >  5:210523/1‑148 (MQ=255)                                                                                                                        cAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCGGCGAGc                          <  2:5359/149‑1 (MQ=255)                                                                                                                         aGCGCGCCAATCTGGTTTACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTTGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCGGCGAGCg                         <  1:17144/149‑1 (MQ=255)                                                                                                                          gcgcgcCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCGGCGAGCgg                        <  5:295239/149‑1 (MQ=255)                                                                                                                          gcgcgcCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCGGCGAGCgg                        <  7:349111/149‑1 (MQ=255)                                                                                                                              gcCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCGGCGAGCGGGCAt                    <  7:264798/149‑1 (MQ=255)                                                                                                                              gcCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCGGCGAGCGGGCAt                    <  8:200718/149‑1 (MQ=255)                                                                                                                              gcCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCGGCGAGCGGGCAt                    <  8:229158/149‑1 (MQ=255)                                                                                                                                     tGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCGGCGAGCGGGCATGGAAAAt             >  1:260325/1‑149 (MQ=255)                                                                                                                                       gTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCGGCGAGCGGGCATGGAAAATCt           >  1:331862/1‑149 (MQ=255)                                                                                                                                        ccgACCAGGCCACCTTGGGTGGGCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCGGCGAGCGGGCATGGAAAATCt           >  8:157582/3‑148 (MQ=255)                                                                                                                                          gACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCGGCGAGCGGGCATGGAAAATCTGCt        >  7:103507/1‑149 (MQ=255)                                                                                                                                           aCCAGGCCACCTTGGGTGGGCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCGGCGAGCGGGCATGGAAAATCTGCTc       >  5:204084/1‑149 (MQ=255)                                                                                                                                           aCCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCGGCGAGCGGGCATGGAAAATCTGCTc       <  6:63889/149‑1 (MQ=255)                                                                                                                                            ccAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCGGCGAGCGGGCATGGAAAATCTGCTc       >  4:143588/1‑148 (MQ=255)                                                                                                                                               gCCGACCCAAGGTGGGCTTGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTCCCGGCGAGCGGGCATGGAAAATCTGCTCGATc   <  5:290906/149‑1 (MQ=255)                                                                                                                                                cccacccaAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCGGCGAGCGGGCATGGAAAATCTTCTCGATCa  >  5:152107/1‑149 (MQ=255)                                                                                                                                                |                                                                                                                                                     AACTCCCAGGCCTTGAGGATCTTGTCCCCCAGCACCGGCTGGAGTTGCTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCGGCGAGCGGGCATGGAAAATCTGCTCGATCA  >  NC_002947/1622764‑1623055

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑