Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,622,915 A→G 6.4% L148L (TTG→CTG) ‡ PP_1422 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,622,9150AG 6.4% 183.9 / 2.6 64L148L (TTG→CTG) ‡PP_1422hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (30/28);  new base G (2/2);  total (33/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.75e-01

CCAGGCCTTGAGGATCTTGTCCCCCAGCACCGGCTGGAGTTGCTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCGGCGAGCGGGCATGGAAAATCTGCTCGATCACCAGGCC  >  NC_002947/1622769‑1623062
                                                                                                                                                  |                                                                                                                                                   
ccAGGCCTTGAGGATCTTGTCCCCCAGCACCGGCTGGAGTTGCTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGGATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAgg                                                                                                                                                   >  7:204268/1‑149 (MQ=255)
ccAGGCCTTGAGGATCTTGTCCCCCAGCACCGGCTGGAGTTGCTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAg                                                                                                                                                    <  8:129712/148‑1 (MQ=255)
 cAGGCCTTGAGGATCTTGTCCCCCAGCACCGGCTGGAGTTGCTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGt                                                                                                                                                  >  2:54729/1‑149 (MQ=255)
         gAGGATCTTGTCCCCCAGCACCGGCTGGAGTTGCTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGGCCACCTTGGGTGGGCTgg                                                                                                                                           <  4:370461/148‑1 (MQ=255)
           ggAACTTGTCCCCCAGCAGCGGCTGGAGTTGCTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGATCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCAAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCg                                                                                                                                        >  6:290906/1‑149 (MQ=255)
                  gTCCCCCAGCACCGGCTGGAGTTGCTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGGCCACCTTGGGTGGGCTGGTcaac                                                                                                                                      <  7:50037/144‑4 (MQ=255)
                   tCCCCCAGCACCGGCTGGAGTTGCTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTgag                                                                                                                                <  1:76930/149‑1 (MQ=255)
                      cccAGCACCGGCTGGAGTTGCTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAgg                                                                                                                             >  2:93503/1‑149 (MQ=255)
                       ccAGCACCGGCTGGAGTTGCTCGAGCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGgt                                                                                                                            <  6:263118/149‑1 (MQ=255)
                        cAGCACCGGCTGGAGTTGCTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGgtg                                                                                                                           >  1:28513/1‑149 (MQ=255)
                          gCACCGGCTGGAGTTGCTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGgtgt                                                                                                                          >  1:5359/1‑148 (MQ=255)
                                 cTGGAGTTGCTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGACGGGTTTGAGAGGTGTGAAGCGAc                                                                                                                  <  5:143984/149‑1 (MQ=255)
                                  gggAGTTGCTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGTCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCATCCCACCCAAGGTGGGCTGGTCGGGTTTGAGAGGTGTGAAGCGACg                                                                                                                 >  5:267326/2‑149 (MQ=255)
                                    gAGTTGCTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCgg                                                                                                                                       >  5:18067/1‑125 (MQ=255)
                                    gAGTTGCTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCgg                                                                                                                                       <  6:18067/125‑1 (MQ=255)
                                    gAGTTGCTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAACGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACgg                                                                                                                >  7:229158/1‑148 (MQ=255)
                                     aGTTGCTCGATCACATAATGCAGGCAAACCGGGTCGGACAAGAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCa                                                                                                              <  5:324169/149‑1 (MQ=255)
                                       ttGCTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACgg                                                                                                                >  5:313901/1‑145 (MQ=255)
                                         gCTCGATCACATAATGCAGGCAAACCGACTAGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGCAAGCGCGCAAATCTGGTTGACCACCAAACCCAACGTCGCCTGGTCCGGTTTCAGAGGTGTGAAGAGACGGCAGAt                                                                                                           >  2:17144/1‑148 (MQ=255)
                                          cTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCTGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAATGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGAttt                                                                                                         <  5:23565/149‑1 (MQ=255)
                                              aTCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTa                                                                                                     <  1:93503/149‑1 (MQ=255)
                                              aTCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTa                                                                                                     <  2:28513/149‑1 (MQ=255)
                                              aTCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTa                                                                                                     <  1:54729/149‑1 (MQ=255)
                                                 acaTAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGc                                                                                                   <  6:219422/148‑1 (MQ=255)
                                                    tAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGAt                                                                                               >  7:145632/1‑149 (MQ=255)
                                                      aTGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATg                                                                                              <  3:326421/148‑1 (MQ=255)
                                                        gCAGGCAAACCGGGTCGGAAAACAGTTCGTAGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGATTGAGAGGTGTGAACCGACGGCAGAATTCCGAGCTGATGccc                                                                                           >  2:139949/1‑149 (MQ=255)
                                                         cAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGccc                                                                                           <  1:71064/148‑1 (MQ=255)
                                                         cAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCg                                                                                          >  4:356664/1‑149 (MQ=255)
                                                           ggCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGcc                                                                                        <  6:313901/149‑1 (MQ=255)
                                                           ggCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGcc                                                                                        <  3:108621/149‑1 (MQ=255)
                                                                  cGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCa                                                                                 <  7:85720/149‑1 (MQ=255)
                                                                         gACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGAGCCAATCTGGTTGACCAGCCCAACCACGGTGGCCTGGTAGGGGTTGAGAGTTGTGAAGCGCCCGCAGAATTCGTAGCTGATGCCCGCCACTTCCAGTCTaat                                                                          >  5:57213/1‑147 (MQ=255)
                                                                                gTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGcc                                                                      >  5:63889/1‑146 (MQ=255)
                                                                                    gTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGatat                                                               >  8:349111/1‑149 (MQ=255)
                                                                                       gtgCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCAGCCATGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATct                                                             <  1:139949/148‑2 (MQ=255)
                                                                                         gCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATcgcg                                                           >  1:53995/1‑148 (MQ=255)
                                                                                                  cATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCCCCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTATCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCCCAGTTTCTGc                                                 >  6:296198/1‑149 (MQ=255)
                                                                                                  cATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGc                                                 >  6:295239/1‑149 (MQ=255)
                                                                                                       aTGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTATCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTATGCTCCa                                             >  8:276383/1‑148 (MQ=255)
                                                                                                         gAGTACCGGAAGCGCGCCAATCTGGTTGAGCAGCCCACCCAAGGTGGCCTGGTCGTGTTTGAGAGGTGTGAAGCGACTGCAGATTTCGTAGCTGATGCCCGCCCCTTCCAGTCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACt                                           <  1:78759/148‑1 (MQ=255)
                                                                                                            tACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCg                                       >  5:56458/1‑149 (MQ=255)
                                                                                                              ccGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCGGc                                     >  5:270487/1‑149 (MQ=255)
                                                                                                               cGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGccc                                                                     <  8:24461/116‑1 (MQ=255)
                                                                                                               cGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGccc                                                                     >  7:24461/1‑116 (MQ=255)
                                                                                                               cGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCGGc                                     >  5:210523/1‑148 (MQ=255)
                                                                                                                  cAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCGGCGAGc                                 <  2:5359/149‑1 (MQ=255)
                                                                                                                   aGCGCGCCAATCTGGTTTACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTTGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCGGCGAGCg                                <  1:17144/149‑1 (MQ=255)
                                                                                                                    gcgcgcCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCGGCGAGCgg                               <  7:349111/149‑1 (MQ=255)
                                                                                                                    gcgcgcCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCGGCGAGCgg                               <  5:295239/149‑1 (MQ=255)
                                                                                                                        gcCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCGGCGAGCGGGCAt                           <  7:264798/149‑1 (MQ=255)
                                                                                                                        gcCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCGGCGAGCGGGCAt                           <  8:200718/149‑1 (MQ=255)
                                                                                                                        gcCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCGGCGAGCGGGCAt                           <  8:229158/149‑1 (MQ=255)
                                                                                                                               tGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCGGCGAGCGGGCATGGAAAAt                    >  1:260325/1‑149 (MQ=255)
                                                                                                                                 gTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCGGCGAGCGGGCATGGAAAATCt                  >  1:331862/1‑149 (MQ=255)
                                                                                                                                  ccgACCAGGCCACCTTGGGTGGGCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCGGCGAGCGGGCATGGAAAATCt                  >  8:157582/3‑148 (MQ=255)
                                                                                                                                    gACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCGGCGAGCGGGCATGGAAAATCTGCt               >  7:103507/1‑149 (MQ=255)
                                                                                                                                     aCCAGGCCACCTTGGGTGGGCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCGGCGAGCGGGCATGGAAAATCTGCTc              >  5:204084/1‑149 (MQ=255)
                                                                                                                                     aCCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCGGCGAGCGGGCATGGAAAATCTGCTc              <  6:63889/149‑1 (MQ=255)
                                                                                                                                      ccAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCGGCGAGCGGGCATGGAAAATCTGCTc              >  4:143588/1‑148 (MQ=255)
                                                                                                                                         gCCGACCCAAGGTGGGCTTGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTCCCGGCGAGCGGGCATGGAAAATCTGCTCGATc          <  5:290906/149‑1 (MQ=255)
                                                                                                                                          cccacccaAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCGGCGAGCGGGCATGGAAAATCTTCTCGATCa         >  5:152107/1‑149 (MQ=255)
                                                                                                                                              cccaAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCGGCGAGCGGGCATGGAAAATCTGCTCGATCACCAg     <  8:204268/149‑1 (MQ=255)
                                                                                                                                               ccaAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCGGCGAGCGGGCATGGAAAATCTGCTCGATCACCAgg    >  3:262138/1‑149 (MQ=255)
                                                                                                                                                 aaGGTGGCCTGCTCGGGTTTGAGAGGTGTGAAGCGACCGCAGATTTCGAAGCTGAAGCCCGCCACTTCCAGGCTACTGGCCCAGATATCCCGCAGTTTCTGCTCCACTGCCGGCGAGCGGGCATGGAAAATCTGCTCGATCACCAGGcc  >  2:286808/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
CCAGGCCTTGAGGATCTTGTCCCCCAGCACCGGCTGGAGTTGCTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCGGCGAGCGGGCATGGAAAATCTGCTCGATCACCAGGCC  >  NC_002947/1622769‑1623062

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑