Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,622,921	C→G	9.3%	A146P (GCC→CCC)	PP_1422 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,622,921	0	C	G	9.3%	178.2 / 5.7	62	A146P (GCC→CCC)	PP_1422	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (29/27);  new base G (3/3);  total (32/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.71e-01

GAGGATCTTGTCCCCCAGCACCGGCTGGAGTTGCTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCGGCGAGCGGGCATGGAAAATCTGCTCGATCACCAGGCC  >  NC_002947/1622778‑1623062                                                                                                                                                |                                                                                                                                              gAGGATCTTGTCCCCCAGCACCGGCTGGAGTTGCTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGGCCACCTTGGGTGGGCTgg                                                                                                                                           <  4:370461/148‑1 (MQ=255)   ggAACTTGTCCCCCAGCAGCGGCTGGAGTTGCTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGATCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCAAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCg                                                                                                                                        >  6:290906/1‑149 (MQ=255)          gTCCCCCAGCACCGGCTGGAGTTGCTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGGCCACCTTGGGTGGGCTGGTcaac                                                                                                                                      <  7:50037/144‑4 (MQ=255)           tCCCCCAGCACCGGCTGGAGTTGCTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTgag                                                                                                                                <  1:76930/149‑1 (MQ=255)              cccAGCACCGGCTGGAGTTGCTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAgg                                                                                                                             >  2:93503/1‑149 (MQ=255)               ccAGCACCGGCTGGAGTTGCTCGAGCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGgt                                                                                                                            <  6:263118/149‑1 (MQ=255)                cAGCACCGGCTGGAGTTGCTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGgtg                                                                                                                           >  1:28513/1‑149 (MQ=255)                  gCACCGGCTGGAGTTGCTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGgtgt                                                                                                                          >  1:5359/1‑148 (MQ=255)                         cTGGAGTTGCTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGACGGGTTTGAGAGGTGTGAAGCGAc                                                                                                                  <  5:143984/149‑1 (MQ=255)                          gggAGTTGCTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGTCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCATCCCACCCAAGGTGGGCTGGTCGGGTTTGAGAGGTGTGAAGCGACg                                                                                                                 >  5:267326/2‑149 (MQ=255)                            gAGTTGCTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCgg                                                                                                                                       <  6:18067/125‑1 (MQ=255)                            gAGTTGCTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCgg                                                                                                                                       >  5:18067/1‑125 (MQ=255)                            gAGTTGCTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAACGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACgg                                                                                                                >  7:229158/1‑148 (MQ=255)                             aGTTGCTCGATCACATAATGCAGGCAAACCGGGTCGGACAAGAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCa                                                                                                              <  5:324169/149‑1 (MQ=255)                               ttGCTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACgg                                                                                                                >  5:313901/1‑145 (MQ=255)                                 gCTCGATCACATAATGCAGGCAAACCGACTAGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGCAAGCGCGCAAATCTGGTTGACCACCAAACCCAACGTCGCCTGGTCCGGTTTCAGAGGTGTGAAGAGACGGCAGAt                                                                                                           >  2:17144/1‑148 (MQ=255)                                  cTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCTGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAATGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGAttt                                                                                                         <  5:23565/149‑1 (MQ=255)                                      aTCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTa                                                                                                     <  2:28513/149‑1 (MQ=255)                                      aTCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTa                                                                                                     <  1:93503/149‑1 (MQ=255)                                      aTCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTa                                                                                                     <  1:54729/149‑1 (MQ=255)                                         acaTAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGc                                                                                                   <  6:219422/148‑1 (MQ=255)                                            tAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGAt                                                                                               >  7:145632/1‑149 (MQ=255)                                              aTGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATg                                                                                              <  3:326421/148‑1 (MQ=255)                                                gCAGGCAAACCGGGTCGGAAAACAGTTCGTAGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGATTGAGAGGTGTGAACCGACGGCAGAATTCCGAGCTGATGccc                                                                                           >  2:139949/1‑149 (MQ=255)                                                 cAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGccc                                                                                           <  1:71064/148‑1 (MQ=255)                                                 cAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCg                                                                                          >  4:356664/1‑149 (MQ=255)                                                   ggCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGcc                                                                                        <  6:313901/149‑1 (MQ=255)                                                   ggCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGcc                                                                                        <  3:108621/149‑1 (MQ=255)                                                          cGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCa                                                                                 <  7:85720/149‑1 (MQ=255)                                                                 gACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGAGCCAATCTGGTTGACCAGCCCAACCACGGTGGCCTGGTAGGGGTTGAGAGTTGTGAAGCGCCCGCAGAATTCGTAGCTGATGCCCGCCACTTCCAGTCTaat                                                                          >  5:57213/1‑147 (MQ=255)                                                                        gTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGcc                                                                      >  5:63889/1‑146 (MQ=255)                                                                            gTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGatat                                                               >  8:349111/1‑149 (MQ=255)                                                                               gtgCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCAGCCATGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATct                                                             <  1:139949/148‑2 (MQ=255)                                                                                 gCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATcgcg                                                           >  1:53995/1‑148 (MQ=255)                                                                                          cATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCCCCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTATCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCCCAGTTTCTGc                                                 >  6:296198/1‑149 (MQ=255)                                                                                          cATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGc                                                 >  6:295239/1‑149 (MQ=255)                                                                                               aTGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTATCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTATGCTCCa                                             >  8:276383/1‑148 (MQ=255)                                                                                                 gAGTACCGGAAGCGCGCCAATCTGGTTGAGCAGCCCACCCAAGGTGGCCTGGTCGTGTTTGAGAGGTGTGAAGCGACTGCAGATTTCGTAGCTGATGCCCGCCCCTTCCAGTCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACt                                           <  1:78759/148‑1 (MQ=255)                                                                                                    tACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCg                                       >  5:56458/1‑149 (MQ=255)                                                                                                      ccGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCGGc                                     >  5:270487/1‑149 (MQ=255)                                                                                                       cGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGccc                                                                     <  8:24461/116‑1 (MQ=255)                                                                                                       cGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGccc                                                                     >  7:24461/1‑116 (MQ=255)                                                                                                       cGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCGGc                                     >  5:210523/1‑148 (MQ=255)                                                                                                          cAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCGGCGAGc                                 <  2:5359/149‑1 (MQ=255)                                                                                                           aGCGCGCCAATCTGGTTTACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTTGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCGGCGAGCg                                <  1:17144/149‑1 (MQ=255)                                                                                                            gcgcgcCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCGGCGAGCgg                               <  7:349111/149‑1 (MQ=255)                                                                                                            gcgcgcCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCGGCGAGCgg                               <  5:295239/149‑1 (MQ=255)                                                                                                                gcCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCGGCGAGCGGGCAt                           <  7:264798/149‑1 (MQ=255)                                                                                                                gcCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCGGCGAGCGGGCAt                           <  8:229158/149‑1 (MQ=255)                                                                                                                gcCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCGGCGAGCGGGCAt                           <  8:200718/149‑1 (MQ=255)                                                                                                                       tGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCGGCGAGCGGGCATGGAAAAt                    >  1:260325/1‑149 (MQ=255)                                                                                                                         gTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCGGCGAGCGGGCATGGAAAATCt                  >  1:331862/1‑149 (MQ=255)                                                                                                                          ccgACCAGGCCACCTTGGGTGGGCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCGGCGAGCGGGCATGGAAAATCt                  >  8:157582/3‑148 (MQ=255)                                                                                                                            gACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCGGCGAGCGGGCATGGAAAATCTGCt               >  7:103507/1‑149 (MQ=255)                                                                                                                             aCCAGGCCACCTTGGGTGGGCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCGGCGAGCGGGCATGGAAAATCTGCTc              >  5:204084/1‑149 (MQ=255)                                                                                                                             aCCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCGGCGAGCGGGCATGGAAAATCTGCTc              <  6:63889/149‑1 (MQ=255)                                                                                                                              ccAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCGGCGAGCGGGCATGGAAAATCTGCTc              >  4:143588/1‑148 (MQ=255)                                                                                                                                 gCCGACCCAAGGTGGGCTTGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTCCCGGCGAGCGGGCATGGAAAATCTGCTCGATc          <  5:290906/149‑1 (MQ=255)                                                                                                                                  cccacccaAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCGGCGAGCGGGCATGGAAAATCTTCTCGATCa         >  5:152107/1‑149 (MQ=255)                                                                                                                                      cccaAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCGGCGAGCGGGCATGGAAAATCTGCTCGATCACCAg     <  8:204268/149‑1 (MQ=255)                                                                                                                                       ccaAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCGGCGAGCGGGCATGGAAAATCTGCTCGATCACCAgg    >  3:262138/1‑149 (MQ=255)                                                                                                                                         aaGGTGGCCTGCTCGGGTTTGAGAGGTGTGAAGCGACCGCAGATTTCGAAGCTGAAGCCCGCCACTTCCAGGCTACTGGCCCAGATATCCCGCAGTTTCTGCTCCACTGCCGGCGAGCGGGCATGGAAAATCTGCTCGATCACCAGGcc  >  2:286808/1‑149 (MQ=255)                                                                                                                                                |                                                                                                                                              GAGGATCTTGTCCCCCAGCACCGGCTGGAGTTGCTCGATCACATAATGCAGGCAAACCGGGTCGGACAACAGTTCGTTGTGCTCTTCGGCATAGATGAGTACCGGCAGCGCGCCAATCTGGTTGACCAGCCCACCCAAGGTGGCCTGGTCGGGTTTGAGAGGTGTGAAGCGACGGCAGATTTCGTAGCTGATGCCCGCCACTTCCAGGCTACTGGCCCAGATATCGCGCAGTTTCTGCTCCACTGCCGGCGAGCGGGCATGGAAAATCTGCTCGATCACCAGGCC  >  NC_002947/1622778‑1623062

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑