Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,625,208 A→C 10.8% intergenic (+31/+26) PP_1425 → / ← nadB hypothetical protein/L‑aspartate oxidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,625,2080AC10.8% 124.3 / 4.6 52intergenic (+31/+26)PP_1425/nadBhypothetical protein/L‑aspartate oxidase
Reads supporting (aligned to +/- strand):  ref base A (22/24);  new base C (3/3);  total (25/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01

TGGGGGAAAGCCAGTGTATACCTCAGGATGCGCTAGGGGCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGATCATCAGCTCGGCCAC  >  NC_002947/1625065‑1625353
                                                                                                                                               |                                                                                                                                                 
tGGGGGAAAGCCAGTGTATACCTCAGGATGCGCTAGGGGCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCcg                                                                                                                                              >  7:303142/1‑149 (MQ=255)
    ggAAAGCCAGTGTATACCTCAGGATGCGCTAGGGGCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCcgcg                                                                                                                                            >  7:49471/1‑147 (MQ=255)
    ggAAAGCCAGTGTATACCTCAGGATGCGCTAGGGGCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGaa                                                                                                                                          <  3:355644/149‑1 (MQ=255)
     gAAAGCCAGTGTATACCTCAGGATGCGCTAGGGGCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAg                                                                                                                                         >  6:343464/1‑149 (MQ=255)
     gAAAGCCAGTGTATACCTCAGGATGCGCTAGGGGCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAg                                                                                                                                         <  8:303142/149‑1 (MQ=255)
     gAAAGCCAGTGTATACCTCAGGATGCGCTAGGGGCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAg                                                                                                                                         <  8:209661/149‑1 (MQ=255)
       aaGCCAGTGTATACCTCAGGATGCGCTAGGGGCTGACGAGCATCGGCGCTTGCGGCTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCTCGCCCGCGAAgaa                                                                                                                                       <  7:33046/149‑3 (MQ=255)
         gCCAGTGTATACCTCAGGATGCGCTAGGGGCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTCCGCGGGCGCGCCCGCGAAGGCCa                                                                                                                                     >  5:15192/1‑149 (MQ=255)
         gCCAGTGTATACCTCAGGATGCGCTAGGGGCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTCCGCGGGCGAGACCGCGAAGGCCa                                                                                                                                     >  5:9962/1‑149 (MQ=255)
           cAGTGTATACCTCAGGATGCGCTAGGGGCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCacg                                                                                                                                   >  3:270878/1‑149 (MQ=255)
           cAGTGTATACCTCAGGATGCGCTAGGGGCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCacg                                                                                                                                   >  3:233639/1‑149 (MQ=255)
                  taCCTCAGGTTGGGCTTGGGGCGGACGCGCCTCGGCGCGTGCGGGTGCGGTGTAAGTTCAGCCGGGGGCGCTGGGCTGCGGTCAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTCCGCGGGCGAGCCCGCGAAGGCCACGACGCCg                                                                                                                             <  6:15192/148‑1 (MQ=255)
                  taCCGCAGGATGCGCTAGGGGCTGACGAGCATCGCCGCTTGCGGGTGGGGTTGATGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGa                                                                                                                            <  6:113338/149‑1 (MQ=255)
                   aCCTCAGGATGCGCTAGGGGCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGa                                                                                                                            >  2:51000/1‑148 (MQ=255)
                    ccTCAGGATGCGCTAGGGGCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGat                                                                                                                           <  6:182933/148‑1 (MQ=255)
                     cTCAGGATGCGCTAGGGGCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGatat                                                                                                                         >  3:108410/1‑149 (MQ=255)
                       cAGGATGCGCTAGGGGCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGatat                                                                                                                         >  4:22259/1‑147 (MQ=255)
                              cgcTCGGGGCTGACCTCCAGCGCCGGTGGCGGGGGCGGGTGAAGTTCACCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATCGATCCCGACCGGGTTAGCTTCTTCCCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCgg                                                                                                                 <  4:233639/148‑1 (MQ=255)
                                   ggggCGGACCAGCAGCGGCGCTGGCGGGTGCGGGGGAAGTGCAGCGGGCGGCGCTGGGCTGCGGTGCGCCGGGAATAGATCGAGCCCGGGTAAGCTGCTGCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTaa                                                                                                            <  7:325074/148‑1 (MQ=255)
                                    gggCTGACGTGCATGGGCGCTTGCTGGTGTGTTTTTATTTCTGGCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTTTTGGCGGGCTAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAg                                                                                                           <  8:49471/148‑1 (MQ=255)
                                     ggCTGACGAGCATCGGAGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCAGAAATCAGAGCGTCTTAAgg                                                                                                          >  4:45184/1‑148 (MQ=255)
                                      gCTGACGAGCATCGGCGCTGGCGGGTGCGGTTGAAGTGCAGCCGGCGGCGCGGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGAt                                                                                                        <  1:265941/149‑1 (MQ=255)
                                        tGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGAt                                                                                                        >  3:165019/1‑147 (MQ=255)
                                               cATCGGCGCTTGCGGGTGCGGTGGAAGTTCTCGCGGCGGCGGTGGTCTGCGGGGAGCCGTGCATAGATCGAGACCGGGTTAGCTTCTTCCCGGGCGACCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCtt                                                                                               <  8:117480/149‑1 (MQ=255)
                                                          gCGGGTGCGGTTGAAGTTCAGCCGGGGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTcc                                                                                     <  5:343464/148‑1 (MQ=255)
                                                             ggTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCa                                                                                 >  6:304934/1‑149 (MQ=255)
                                                                         gTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAAt                                                                     >  8:300160/1‑149 (MQ=255)
                                                                          ttCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGGTGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATc                                                                    >  3:232303/1‑149 (MQ=255)
                                                                              gCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAgt                                                                >  8:89741/1‑149 (MQ=255)
                                                                                   gggcgCTGGGCTGCGTTCTGCCGGGAATAGCTCCCCACCGTGTTAGCTTCTTCGGGGGCGAGCCGGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTa                                                           <  7:147895/148‑1 (MQ=255)
                                                                                    ggcgCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTaa                                                          <  2:123679/149‑1 (MQ=255)
                                                                                    ggcgCTGGGCTGCGGTGAGCCGGGAATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTaa                                                          <  4:270878/149‑1 (MQ=255)
                                                                                         tGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCaa                                                     >  6:176081/1‑149 (MQ=255)
                                                                                           ggCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGACGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAAcc                                                   >  1:89573/1‑149 (MQ=255)
                                                                                             cTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCt                                                 >  3:163545/1‑149 (MQ=255)
                                                                                                   tGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCtt                                           <  7:89741/149‑1 (MQ=255)
                                                                                                   tGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCtt                                           <  3:22259/149‑1 (MQ=255)
                                                                                                     cgcggtgCATAGTTGGTGACGTGGTTCGCTTCTTCGGGGGCTCGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTc                                         <  4:163545/143‑1 (MQ=255)
                                                                                                                gATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGc                               <  5:304934/148‑1 (MQ=255)
                                                                                                                  tCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATg                            <  7:300160/149‑1 (MQ=255)
                                                                                                                  tCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATg                            >  5:312403/1‑149 (MQ=255)
                                                                                                                         cGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAg                                                                                                           <  2:79059/63‑1 (MQ=255)
                                                                                                                         cGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAg                                                                                                           >  1:79059/1‑63 (MQ=255)
                                                                                                                                 gttcttcGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCTGCTTTCGTTGCGCTGCATGGCTGACAGGatcat              <  3:45184/147‑1 (MQ=255)
                                                                                                                                 cttcttCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGatcatc             <  4:108410/149‑1 (MQ=255)
                                                                                                                                    cttcGCGGGCTCGCCCGCGAAGGCCACGACGCCGATATGAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTACATGGCTGACAGGATCATCAGc          >  7:194795/1‑149 (MQ=255)
                                                                                                                                    cttcGCGGGCTCGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGATCATCAGc          >  1:27931/1‑149 (MQ=255)
                                                                                                                                       cgcgGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTgcg                                   >  6:222934/1‑121 (MQ=255)
                                                                                                                                       cgcgGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTgcg                                   <  5:222934/121‑1 (MQ=255)
                                                                                                                                       cgcgGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGATCATCAGCTCg       >  4:169163/1‑149 (MQ=255)
                                                                                                                                         cgGGCTCGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTACATGGCTGACAGGATCATCAGCTCGGc     <  8:194795/149‑1 (MQ=255)
                                                                                                                                            ccGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGATCATCAGCTCGGCCAc  <  2:89573/148‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                 
TGGGGGAAAGCCAGTGTATACCTCAGGATGCGCTAGGGGCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGATCATCAGCTCGGCCAC  >  NC_002947/1625065‑1625353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑