Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,661,477 C→A 5.7% A50D (GCC→GAC)  PP_1456 → membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,661,4770CA 5.7% 224.2 / 3.3 70A50D (GCC→GAC) PP_1456membrane protein
Reads supporting (aligned to +/- strand):  ref base C (31/35);  new base A (2/2);  total (33/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.79e-01

TTCTATCTGATTGCCTACATCAGCAGCGTAGTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTCGGCCGCTGCGCGACCGCCTG  >  NC_002947/1661332‑1661625
                                                                                                                                                 |                                                                                                                                                    
atCTATCTGATTGCCTACATCAGCAGCGTAGTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGccc                                                                                                                                                    <  6:58503/147‑1 (MQ=255)
     tCTGATTGCCTACATCAGCAGCGTAGTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGTCGCCCTGGTCg                                                                                                                                              >  8:111928/1‑149 (MQ=255)
         aTTGCCTACATCAGCAGCGTAGTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCCTCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCa                                                                                                                                           <  1:152110/148‑1 (MQ=255)
         aTTGCCTACATCAGCAGCGTAGTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCAt                                                                                                                                          >  8:345177/1‑149 (MQ=255)
         aTTGCCTACATCAGCAGCGTAGTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCAt                                                                                                                                          <  5:353686/149‑1 (MQ=255)
          ttGCCTACATCAGCAGCGTAGTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCATGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCagg                                                                                                                                         >  8:68640/1‑147 (MQ=255)
            gCCTACATCAGCAGCGTAGTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGACCAGGGCGCCATga                                                                                                                                        <  7:94151/148‑2 (MQ=255)
             ccTACATCAGCAGCGTAGTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGGGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCtg                                                                                                                                      <  5:221385/149‑1 (MQ=255)
               tACATCAGCAGCGTAGTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTCCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCtggt                                                                                                                                    >  5:5214/1‑149 (MQ=255)
                 cATCAGCAGCGTAGTGCTGATCAACAACGCCATCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCtggt                                                                                                                                    <  4:277463/147‑1 (MQ=255)
                              gTGCTGATCAACTACGCCGTCTCCAGTGCCCCGCAGCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCCCTGGTGCTGTc                                                                                                                     <  5:157348/149‑1 (MQ=255)
                                 cTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCt                                                                                                                   <  5:212177/148‑1 (MQ=255)
                                  tGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTCGGGCGGCCTGGTCTTCATCCTGCGCGACATGCTGCAGACCCGTTTTGGTCATCGCGCCCTGGTCGCCATCCTGGTGGCACTGGTGCTGTCCTAt                                                                                                                 >  5:210138/1‑149 (MQ=255)
                                     tCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGt                                                                                                               >  5:159929/1‑148 (MQ=255)
                                      cAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCa                                                                                                             >  7:102231/1‑149 (MQ=255)
                                       aaCTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCAc                                                                                                            <  3:195895/149‑1 (MQ=255)
                                         cTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTc                                                                                                                     >  6:345180/1‑138 (MQ=255)
                                         cTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTc                                                                                                                     <  5:345180/138‑1 (MQ=255)
                                              ccTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAg                                                                                                     >  3:114353/1‑149 (MQ=255)
                                              ccTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAg                                                                                                     >  3:91833/1‑149 (MQ=255)
                                                    ccAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGc                                                                                                >  4:324330/1‑148 (MQ=255)
                                                     cAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCa                                                                                              >  7:33994/1‑149 (MQ=255)
                                                        cgCCCCGCATCTGGACATCCTCTGGTCTCCCTCGGCCGGCCCGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGCTCATGGCGCCCTGGGCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCACCg                                                                                           <  4:268504/148‑1 (MQ=255)
                                                             cGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTg                                                                                      <  4:72059/149‑1 (MQ=255)
                                                              gCATCTGCACATCATGTGGTCTGGCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCtgg                                                                                                                                     <  4:24399/101‑1 (MQ=255)
                                                                aTCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGc                                                                                    >  8:202909/1‑148 (MQ=255)
                                                                    ggACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAgcg                                                                               >  4:253476/1‑149 (MQ=255)
                                                                    ggACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAgcg                                                                               >  1:92075/1‑149 (MQ=255)
                                                                     gACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAgcgc                                                                              >  2:360245/1‑149 (MQ=255)
                                                                        atcatcTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCGTGGCCAGCGCCAc                                                                           >  1:132126/1‑149 (MQ=255)
                                                                         tcatcTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACt                                                                          <  2:106849/149‑1 (MQ=255)
                                                                         tcatcTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACACGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACt                                                                          >  8:197466/1‑149 (MQ=255)
                                                                          catcTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCgg                                                                                                 <  8:133034/125‑1 (MQ=255)
                                                                                gTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGttct                                                                   >  4:279333/1‑149 (MQ=255)
                                                                                 tCTGCCTGTGTCGGCCTGGTTTTCATCCTGCGCGACATGGCGCAGACCCGTTTTTGGCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGttctt                                                                  <  7:176518/149‑1 (MQ=255)
                                                                                 tCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGttctt                                                                  <  6:159929/149‑1 (MQ=255)
                                                                                  cTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGttctt                                                                  >  8:143211/1‑148 (MQ=255)
                                                                                     ccTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGttcttc                                                                 <  5:267917/146‑1 (MQ=255)
                                                                                     ccTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCAtt                                                              >  8:114142/1‑149 (MQ=255)
                                                                                         gggCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGGCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTcc                                                           <  6:5214/148‑1 (MQ=255)
                                                                                           gcggcCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGACCTGGCCAGCGCCACTGCGTTCTTCATTTcc                                                           >  3:224656/1‑146 (MQ=255)
                                                                                            cggcCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGc                                                       <  2:132126/149‑1 (MQ=255)
                                                                                                   gtgtTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATc                                                  <  1:360245/147‑1 (MQ=255)
                                                                                                     gtTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTAGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGAct                                              >  8:232590/1‑149 (MQ=255)
                                                                                                        cATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGActgg                                            <  4:114353/148‑1 (MQ=255)
                                                                                                          tCCTGCGCGACATGGTGCAGACTCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGActggctg                                         <  7:197466/149‑1 (MQ=255)
                                                                                                          tCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCGCTGCGTTCTTCATTTCCGAGCTGATCGActggctg                                         >  5:358301/1‑149 (MQ=255)
                                                                                                          tCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGActggctg                                         >  7:128552/1‑149 (MQ=255)
                                                                                                          tCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGActggctg                                         <  8:102231/149‑1 (MQ=255)
                                                                                                          tCCTGCGCGACATGGTGCAGACCCGTGTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGActggctg                                         <  7:156741/149‑1 (MQ=255)
                                                                                                            cTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGActggctgg                                        >  3:73375/1‑148 (MQ=255)
                                                                                                            cTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGActggctgg                                        <  4:73375/148‑1 (MQ=255)
                                                                                                                gcgACATTGTGCAGACCCGTTTTGTTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTc                                   <  7:345177/149‑1 (MQ=255)
                                                                                                                gcgACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTc                                   <  7:232590/149‑1 (MQ=255)
                                                                                                                 cgACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCa                                  <  7:111928/149‑1 (MQ=255)
                                                                                                                 cgACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCa                                  <  3:279333/149‑1 (MQ=255)
                                                                                                                 cgACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCa                                  <  8:128552/149‑1 (MQ=255)
                                                                                                                        gTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCa                            <  3:324330/148‑1 (MQ=255)
                                                                                                                        gTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCAc                           <  2:92075/149‑1 (MQ=255)
                                                                                                                             gACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTc                      >  2:312877/1‑149 (MQ=255)
                                                                                                                              aCCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTCg                     >  6:50831/1‑149 (MQ=255)
                                                                                                                               cccGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTCg                     <  4:224656/148‑1 (MQ=255)
                                                                                                                                        gTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTCGGCCGCTgcgc           <  7:114142/149‑1 (MQ=255)
                                                                                                                                        gTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTCGGCCGCTgcgc           <  6:304684/149‑1 (MQ=255)
                                                                                                                                         tCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTCGGCCGCTg              >  3:184685/1‑145 (MQ=255)
                                                                                                                                         tCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTCGGCCGCTg              <  4:184685/145‑1 (MQ=255)
                                                                                                                                          cATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTCGGCCGCTGCGCGa         >  3:217314/1‑149 (MQ=255)
                                                                                                                                           aTGGCGACCAGGGCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCAt                              >  7:209690/1‑127 (MQ=255)
                                                                                                                                           aTGGCGACCAGGGCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCAt                              <  8:209690/127‑1 (MQ=255)
                                                                                                                                            tGGCGACCAGGGCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTCGGCCGCTGCGCGAcc       >  3:216660/1‑149 (MQ=255)
                                                                                                                                             ggCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTCGGCCGCTGCGCGACCg      >  8:154247/1‑149 (MQ=255)
                                                                                                                                                gcCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTCGGCCGCTGCGCGACCGCCt   <  4:91833/149‑1 (MQ=255)
                                                                                                                                                 cccTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTCGGCCGCTGCGCGACCGCCtg  >  3:137598/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
TTCTATCTGATTGCCTACATCAGCAGCGTAGTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTCGGCCGCTGCGCGACCGCCTG  >  NC_002947/1661332‑1661625

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑