Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,661,483 T→G 7.4% V52G (GTC→GGC)  PP_1456 → membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,661,4830TG 7.4% 210.9 / 4.4 74V52G (GTC→GGC) PP_1456membrane protein
Reads supporting (aligned to +/- strand):  ref base T (33/35);  new base G (2/4);  total (35/39)
Fisher's exact test for biased strand distribution p-value = 6.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCTGATTGCCTACATCAGCAGCGTAGTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTCGGCCGCTGCGCGACCGCCTGTGGCT  >  NC_002947/1661337‑1661630
                                                                                                                                                  |                                                                                                                                                   
tCTGATTGCCTACATCAGCAGCGTAGTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGTCGCCCTGGTCg                                                                                                                                                   >  8:111928/1‑149 (MQ=255)
    aTTGCCTACATCAGCAGCGTAGTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCCTCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCa                                                                                                                                                <  1:152110/148‑1 (MQ=255)
    aTTGCCTACATCAGCAGCGTAGTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCAt                                                                                                                                               >  8:345177/1‑149 (MQ=255)
    aTTGCCTACATCAGCAGCGTAGTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCAt                                                                                                                                               <  5:353686/149‑1 (MQ=255)
     ttGCCTACATCAGCAGCGTAGTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCATGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCagg                                                                                                                                              >  8:68640/1‑147 (MQ=255)
       gCCTACATCAGCAGCGTAGTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGACCAGGGCGCCATga                                                                                                                                             <  7:94151/148‑2 (MQ=255)
        ccTACATCAGCAGCGTAGTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGGGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCtg                                                                                                                                           <  5:221385/149‑1 (MQ=255)
          tACATCAGCAGCGTAGTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTCCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCtggt                                                                                                                                         >  5:5214/1‑149 (MQ=255)
            cATCAGCAGCGTAGTGCTGATCAACAACGCCATCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCtggt                                                                                                                                         <  4:277463/147‑1 (MQ=255)
                         gTGCTGATCAACTACGCCGTCTCCAGTGCCCCGCAGCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCCCTGGTGCTGTc                                                                                                                          <  5:157348/149‑1 (MQ=255)
                            cTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCt                                                                                                                        <  5:212177/148‑1 (MQ=255)
                             tGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTCGGGCGGCCTGGTCTTCATCCTGCGCGACATGCTGCAGACCCGTTTTGGTCATCGCGCCCTGGTCGCCATCCTGGTGGCACTGGTGCTGTCCTAt                                                                                                                      >  5:210138/1‑149 (MQ=255)
                                tCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGt                                                                                                                    >  5:159929/1‑148 (MQ=255)
                                 cAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCa                                                                                                                  >  7:102231/1‑149 (MQ=255)
                                  aaCTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCAc                                                                                                                 <  3:195895/149‑1 (MQ=255)
                                    cTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTc                                                                                                                          >  6:345180/1‑138 (MQ=255)
                                    cTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTc                                                                                                                          <  5:345180/138‑1 (MQ=255)
                                         ccTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAg                                                                                                          >  3:114353/1‑149 (MQ=255)
                                         ccTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAg                                                                                                          >  3:91833/1‑149 (MQ=255)
                                               ccAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGc                                                                                                     >  4:324330/1‑148 (MQ=255)
                                                cAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCa                                                                                                   >  7:33994/1‑149 (MQ=255)
                                                   cgCCCCGCATCTGGACATCCTCTGGTCTCCCTCGGCCGGCCCGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGCTCATGGCGCCCTGGGCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCACCg                                                                                                <  4:268504/148‑1 (MQ=255)
                                                        cGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTg                                                                                           <  4:72059/149‑1 (MQ=255)
                                                         gCATCTGCACATCATGTGGTCTGGCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCtgg                                                                                                                                          <  4:24399/101‑1 (MQ=255)
                                                           aTCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGc                                                                                         >  8:202909/1‑148 (MQ=255)
                                                               ggACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAgcg                                                                                    >  1:92075/1‑149 (MQ=255)
                                                               ggACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAgcg                                                                                    >  4:253476/1‑149 (MQ=255)
                                                                gACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAgcgc                                                                                   >  2:360245/1‑149 (MQ=255)
                                                                   atcatcTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCGTGGCCAGCGCCAc                                                                                >  1:132126/1‑149 (MQ=255)
                                                                    tcatcTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACt                                                                               <  2:106849/149‑1 (MQ=255)
                                                                    tcatcTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACACGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACt                                                                               >  8:197466/1‑149 (MQ=255)
                                                                     catcTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCgg                                                                                                      <  8:133034/125‑1 (MQ=255)
                                                                           gTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGttct                                                                        >  4:279333/1‑149 (MQ=255)
                                                                            tCTGCCTGTGTCGGCCTGGTTTTCATCCTGCGCGACATGGCGCAGACCCGTTTTTGGCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGttctt                                                                       <  7:176518/149‑1 (MQ=255)
                                                                            tCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGttctt                                                                       <  6:159929/149‑1 (MQ=255)
                                                                             cTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGttctt                                                                       >  8:143211/1‑148 (MQ=255)
                                                                                ccTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGttcttc                                                                      <  5:267917/146‑1 (MQ=255)
                                                                                ccTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCAtt                                                                   >  8:114142/1‑149 (MQ=255)
                                                                                    gggCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGGCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTcc                                                                <  6:5214/148‑1 (MQ=255)
                                                                                      gcggcCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGACCTGGCCAGCGCCACTGCGTTCTTCATTTcc                                                                >  3:224656/1‑146 (MQ=255)
                                                                                       cggcCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGc                                                            <  2:132126/149‑1 (MQ=255)
                                                                                              gtgtTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATc                                                       <  1:360245/147‑1 (MQ=255)
                                                                                                gtTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTAGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGAct                                                   >  8:232590/1‑149 (MQ=255)
                                                                                                   cATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGActgg                                                 <  4:114353/148‑1 (MQ=255)
                                                                                                     tCCTGCGCGACATGGTGCAGACTCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGActggctg                                              <  7:197466/149‑1 (MQ=255)
                                                                                                     tCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCGCTGCGTTCTTCATTTCCGAGCTGATCGActggctg                                              >  5:358301/1‑149 (MQ=255)
                                                                                                     tCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGActggctg                                              <  8:102231/149‑1 (MQ=255)
                                                                                                     tCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGActggctg                                              >  7:128552/1‑149 (MQ=255)
                                                                                                     tCCTGCGCGACATGGTGCAGACCCGTGTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGActggctg                                              <  7:156741/149‑1 (MQ=255)
                                                                                                       cTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGActggctgg                                             <  4:73375/148‑1 (MQ=255)
                                                                                                       cTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGActggctgg                                             >  3:73375/1‑148 (MQ=255)
                                                                                                           gcgACATTGTGCAGACCCGTTTTGTTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTc                                        <  7:345177/149‑1 (MQ=255)
                                                                                                           gcgACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTc                                        <  7:232590/149‑1 (MQ=255)
                                                                                                            cgACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCa                                       <  7:111928/149‑1 (MQ=255)
                                                                                                            cgACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCa                                       <  8:128552/149‑1 (MQ=255)
                                                                                                            cgACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCa                                       <  3:279333/149‑1 (MQ=255)
                                                                                                                   gTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCa                                 <  3:324330/148‑1 (MQ=255)
                                                                                                                   gTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCAc                                <  2:92075/149‑1 (MQ=255)
                                                                                                                        gACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTc                           >  2:312877/1‑149 (MQ=255)
                                                                                                                         aCCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTCg                          >  6:50831/1‑149 (MQ=255)
                                                                                                                          cccGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTCg                          <  4:224656/148‑1 (MQ=255)
                                                                                                                                   gTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTCGGCCGCTgcgc                <  7:114142/149‑1 (MQ=255)
                                                                                                                                   gTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTCGGCCGCTgcgc                <  6:304684/149‑1 (MQ=255)
                                                                                                                                    tCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTCGGCCGCTg                   >  3:184685/1‑145 (MQ=255)
                                                                                                                                    tCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTCGGCCGCTg                   <  4:184685/145‑1 (MQ=255)
                                                                                                                                     cATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTCGGCCGCTGCGCGa              >  3:217314/1‑149 (MQ=255)
                                                                                                                                      aTGGCGACCAGGGCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCAt                                   >  7:209690/1‑127 (MQ=255)
                                                                                                                                      aTGGCGACCAGGGCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCAt                                   <  8:209690/127‑1 (MQ=255)
                                                                                                                                       tGGCGACCAGGGCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTCGGCCGCTGCGCGAcc            >  3:216660/1‑149 (MQ=255)
                                                                                                                                        ggCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTCGGCCGCTGCGCGACCg           >  8:154247/1‑149 (MQ=255)
                                                                                                                                           gcCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTCGGCCGCTGCGCGACCGCCt        <  4:91833/149‑1 (MQ=255)
                                                                                                                                            cccTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTCGGCCGCTGCGCGACCGCCtg       >  3:137598/1‑149 (MQ=255)
                                                                                                                                               tGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTCGGCCGCTGCGCGACCGCCTGTgg    >  5:218671/1‑149 (MQ=255)
                                                                                                                                                 gTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTCGGCCGCTGCGCGACCGCCTGTGGCt  <  5:374010/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
TCTGATTGCCTACATCAGCAGCGTAGTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCGCCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTCGGCCGCTGCGCGACCGCCTGTGGCT  >  NC_002947/1661337‑1661630

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑