Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	81,979	A→G	7.9%	I97V (ATC→GTC)	PP_0071 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	81,979	0	A	G	7.9%	249.0 / 7.6	88	I97V (ATC→GTC)	PP_0071	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (35/46);  new base G (3/4);  total (38/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

TCCACGTAGCCCTATACCTGCGCAGCGCTTACGAAACCGGCTACAAAGCTTTGGACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCCCGCGAACACCGGCAAAGCCGGTGCCATCCATCGCGGTGGCTTCTTCGCGGGTAAACCC  >  NC_002947/81833‑82126                                                                                                                                                   |                                                                                                                                                    tCCACGTAGCCCTCTACCTGCGCAGCGCTTACGAAACCGGCTACAAAGCTTTGGACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATc                                                                                                                                                   <  8:348685/149‑1 (MQ=255)   cACGTAGCCCTATACCTGCGCAGCGCTTACGAAACCGGCTACAAAGCTTTGGACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATc                                                                                                                                                   >  6:183367/1‑147 (MQ=255)   cACGTAGCCCTATACCTGCGCAGCGCTTACGAAACCGGCTACAAAGCTTTGGACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGa                                                                                                                                                 <  1:128996/149‑1 (MQ=255)    aCGTAGCCCTATACCTGCGCAGCGCTTACGAAACCGGCTACAAAGCTTTGGACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAg                                                                                                                                                >  3:150775/1‑149 (MQ=255)      gTAGCCCTATACCTGCGCAGCGCTTACGAAACCGGCTACAAAGCTTTGGACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAGCCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTc                                                                                                                                              >  7:331451/1‑149 (MQ=255)      gTAGCCCTATACCTGCGCAGCGCTTACGAAACCGGCTACAAAGCTTTGGACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTc                                                                                                                                              <  5:183367/149‑1 (MQ=255)         gCCCTATCCCTGCGCAGCGCTTCCGCAACCGGCTACAAAGCTTTGGACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGAGCGAGGCGATACTCGATGGCATCGAGTCGAc                                                                                                                                           <  5:347908/149‑1 (MQ=255)         gCCCTATACCTGCGCAGCGCTTACGAAACCGGCTACAAAGCTTTGGACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGAc                                                                                                                                           <  6:381581/149‑1 (MQ=255)          cccTATACCTGCGCAGCGCTTACGAAACCGGCTACAAAGCTTTGGACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGAc                                                                                                                                           <  7:268251/148‑1 (MQ=255)          cccTATACCTGCGCAGCGCTTACGAAACCGGCTACAAAGCTTTGGACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACg                                                                                                                                          <  7:295564/149‑1 (MQ=255)           ccTAAACCTGCGCAGCGCTTACGAAACCGGCTACAAAGCTTTGGACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCTATGGCATCGAGTCGACGc                                                                                                                                         >  4:20569/1‑149 (MQ=255)                cccTGCGCAGCGCTTACGAAACCGGCTACAAAGCTTTGGACAACATGCGGTAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCGTCGACTCGATGCCATCg                                                                                                                                    <  8:209069/148‑1 (MQ=255)                 ccTGCGCAGCGCTTACGAAACCGGCTACAAAGCTTTGGACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGTGTGTGATCGAGGCGATACTCGATGGCGTCGACTCGATGCCATCga                                                                                                                                   <  8:185108/149‑2 (MQ=255)                 ccTGCGCAGCGCTTACGAAACCGGCTACAAAGCTTTGGACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGAGTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCGTCGACTCGATGCCATCga                                                                                                                                   <  7:74471/149‑2 (MQ=255)                 ccTGCGAATCGCTTACGAAACCGGCTACAAATCTTTTGACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCTAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCTATACTCGATTGCGTCGACTCGATGCCATCga                                                                                                                                   <  1:374607/149‑2 (MQ=255)                  cTGCGCAGCGCTTACGAAACCGGCTACAAAGCTTTGGACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGcc                                                                                                                                  >  3:218561/1‑149 (MQ=255)                  cTGCGCAGCGCTTACGAAACCGGCTACAAAGCTTTGGACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGcc                                                                                                                                  >  1:27581/1‑149 (MQ=255)                    gcgcAGCGCTTACGAAACCGGCTACAAAGCTTTGGACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGAGTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGt                                                                                                                                >  6:22726/1‑149 (MQ=255)                      gcAGCGCTTACGAAACCGGCTACAAAGCTTTGGACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCa                                                                                                                              <  4:178442/149‑1 (MQ=255)                       cAGCGCTTACGAAACCGGATACAAAGCTTTGGACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCAc                                                                                                                             >  1:341722/1‑149 (MQ=255)                           gcTTACGAAACCGGCTACAAAGCTTTGGACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGcc                                                                                                                          >  8:202344/1‑148 (MQ=255)                            cTTACGAAACCGGCTACAAAGCTTTGGACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCa                                                                                                                        <  8:253311/149‑1 (MQ=255)                             ttACGAAACCGGCTACAAAGCTTTGGACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAg                                                                                                                       >  5:115145/1‑149 (MQ=255)                               aCGAAACCGGCTACAAAGCTTTGGACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGcc                                                                                                                     >  1:45556/1‑149 (MQ=255)                               aCGAAACCGGCTACAAAGCTTTGGACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGc                                                                                                                      >  2:375528/1‑148 (MQ=255)                                 gAAACCGGCTACAAAGCTTTGGACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTc                                                                                                                   >  4:2931/1‑149 (MQ=255)                                    aCCGGCTACAAAGCTTTGGACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCaa                                                                                                                <  7:202414/149‑1 (MQ=255)                                    aCCGGCTACAAAGCTTTGGACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCaa                                                                                                                <  5:42197/149‑1 (MQ=255)                                          tACAAAGCTTTGGACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCa                                                                                                            >  1:190277/1‑147 (MQ=255)                                            cAAAGCTTTGGACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAgc                                                                                                        <  2:94158/149‑1 (MQ=255)                                             aaaGCTTTGGACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAgcg                                                                                                       <  6:128510/149‑1 (MQ=255)                                             aaaGCTTTGGACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCACCAAGGCATAgcg                                                                                                       >  4:57195/1‑149 (MQ=255)                                              aaGCTTTGGACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAgcgc                                                                                                      <  8:263624/149‑1 (MQ=255)                                               aGCTTTGGACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCgct                                                                                                     >  6:220079/1‑149 (MQ=255)                                                 cTTTGGACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCgctg                                                                                                    <  1:375528/148‑1 (MQ=255)                                                      gacgtaaTGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGTTACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATc                                                                                               <  6:259093/142‑1 (MQ=255)                                                      gACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCTTGCTATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCGAATGCATAGCGCTGCTATc                                                                                               <  3:214885/148‑1 (MQ=255)                                                      gACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATc                                                                                               <  3:182946/148‑1 (MQ=255)                                                       aCTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGa                                                                                             <  3:20569/149‑1 (MQ=255)                                                       aCTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGa                                                                                             <  3:6094/149‑1 (MQ=255)                                                        cTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAg                                                                                            <  6:285906/149‑1 (MQ=255)                                                           cATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAgg                                                                                           <  3:284105/147‑1 (MQ=255)                                                            aTGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAgcg                                                                                                       <  2:298581/134‑1 (MQ=255)                                                            aTGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAgcg                                                                                                       >  1:298581/1‑134 (MQ=255)                                                            aTGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGcc                                                                                         >  1:101286/1‑148 (MQ=255)                                                             tGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGa                                                                                       <  4:330144/149‑1 (MQ=255)                                                             tGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGa                                                                                       <  8:324869/149‑1 (MQ=255)                                                               cGTGAAGAAGGCGGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACt                                                                                     <  8:83077/149‑1 (MQ=255)                                                               cGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACt                                                                                     <  7:202344/149‑1 (MQ=255)                                                                 tGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCa                                                                                   <  3:99194/149‑1 (MQ=255)                                                                         gCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAgg                                                                                                              <  5:161993/114‑1 (MQ=255)                                                                         gCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGACCAGCCTCCAAgg                                                                                                              >  6:161993/1‑114 (MQ=255)                                                                                      cTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTg                                                              >  7:37927/1‑149 (MQ=255)                                                                                            gAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATTGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCCcgcg                                                        <  4:218561/149‑1 (MQ=255)                                                                                            gAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCCcgc                                                         >  5:197630/1‑148 (MQ=255)                                                                                            gAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCCcgc                                                         >  1:39790/1‑148 (MQ=255)                                                                                            gAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCCcgc                                                         <  3:44432/148‑1 (MQ=255)                                                                                            gAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAACGCATAGCGCTCGTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCCcgc                                                         >  7:311567/1‑148 (MQ=255)                                                                                             aaCCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCCcgcg                                                        >  1:180519/1‑148 (MQ=255)                                                                                                cTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCCCGCGAaca                                                    >  3:292804/1‑149 (MQ=255)                                                                                                   cATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCCCGCGAacac                                                   <  2:190277/147‑1 (MQ=255)                                                                                                   cATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCCCGCGAACAcc                                                  <  2:101286/148‑1 (MQ=255)                                                                                                     tgggATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTTTCGAGGCCGACTCAGTACCTCTCCGAGCGCGCGAGCCCGCGAACACCgg                                                >  1:110488/4‑148 (MQ=255)                                                                                                       cGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCCCGCGAACACCGGCa                                              <  3:2931/148‑1 (MQ=255)                                                                                                         aTTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCCCGCGAACACCGGCAAAg                                           <  4:292804/149‑1 (MQ=255)                                                                                                              gaTGGCAGCGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCCCGCGAACACCGGCAAAGCCGGt                                      <  7:72071/149‑1 (MQ=255)                                                                                                              gaTGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCCCGCGAACACCGGCAAAGCCGGt                                      <  5:22726/149‑1 (MQ=255)                                                                                                               aTGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCCCGCGAACACCGGCAAAGCCGGTg                                     >  7:196281/1‑149 (MQ=255)                                                                                                                  gCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCCCGCGAACACCGGCAAAGCCGGTGcc                                   <  5:232229/148‑1 (MQ=255)                                                                                                                    agagGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCCCGCGAACACCGGCAAAGCCGGTGccatc                                >  7:28413/1‑149 (MQ=255)                                                                                                                     gagGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCCCGCGAACACCGGc                                               <  2:220315/133‑1 (MQ=255)                                                                                                                     gagGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCCCGCGAACACCGGc                                               >  1:220315/1‑133 (MQ=255)                                                                                                                     gagGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCCCGCGAACACCGGCTTTGCCGGTgttcgcg                               <  2:27581/149‑7 (MQ=39)                                                                                                                              aTCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCCCGCGAACACCGGCAAAGCCGGTGCCATCCATCGCGGt                       <  8:256869/148‑1 (MQ=255)                                                                                                                                 gAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCCCGCGAACACCGGCAAAGCCGGTGCCATCCATCGCGGTGGct                   >  5:48816/1‑149 (MQ=255)                                                                                                                                   ggCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCCCGCGAACACCGGCAAAGCCGGTGCCATCCATCGCGGTGGcttc                 >  3:349551/1‑149 (MQ=255)                                                                                                                                    gCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCCCGCGAACACCGGCAAAGCCGGTGCCATCCATCGCGGTGGcttct                >  6:32977/1‑149 (MQ=255)                                                                                                                                     cGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCCCGCGAACACCGGCAAAGCCGGTGCCATCCATCGCGGTGGcttctt               <  8:37927/149‑1 (MQ=255)                                                                                                                                         aCTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCCCGCGAACACCGGCAAAGCCGGTGCCATCCATCGCGGTGGCTTCTTcgcg           >  7:114416/1‑149 (MQ=255)                                                                                                                                         aCTCGATGCCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCCCGCGAACACCGGCAAAGCCGGTGCCATCCATCGAGGTGGCTTCTTcgcg           >  2:221258/1‑149 (MQ=255)                                                                                                                                          cTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCCCGCGAACACCGGCAAAGCCGGTGCCATCCATCGCGGTGGCTTCTTCGCgg          <  8:114416/149‑1 (MQ=255)                                                                                                                                            cGATGGCGTCGACTCGATGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCCCGCGAACACCGGCAAAGCCGGTGCCATCCATCGCGGTGGCTTCTTCGCggg         >  1:290417/1‑148 (MQ=255)                                                                                                                                             gATGGCGTCGACTCGATGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCACGCGAACACCGGCAAAGCCGGTGCCATCCATCGCGGTGGCTTCTTCGCGGGt        >  6:3197/1‑148 (MQ=255)                                                                                                                                              aTGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCCCGCGAACACCGGCAAAGCCGGTGCCATCCATCGCGGTGGCTTCTTCGCGGGTaa      <  2:180519/149‑1 (MQ=255)                                                                                                                                               tGGCGTCGACTCGATGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCCCGCGAACACCGGCAAAGCCGGTGCCATCCATCGCGGTGGCTTCTTCGCGGGTaaa     >  1:232672/1‑149 (MQ=255)                                                                                                                                                 gCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCCCGCGAACACCGGCAAAGCCGGTGCCATCCATCGCGGTGGCTTCTTCGCGGGTAAAcc   <  5:220079/149‑1 (MQ=255)                                                                                                                                                 gCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCCCGCGAACACCGGCAAAGCCGGTGCCATCCATCGCGGTGGCTTCTTCGCGGGTAAAc    <  2:341722/148‑1 (MQ=255)                                                                                                                                                  cATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCCCGCGAACACCGGCAAAGCCGGTGCCATCCATCGCGGTGGCTTCTTCGCGGGTAAAccc  >  5:32582/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                    TCCACGTAGCCCTATACCTGCGCAGCGCTTACGAAACCGGCTACAAAGCTTTGGACTACATGCGTGAAGAAGGCCGTGGCATGTTCTGGTCGAACCTGCATGCGATTGAGATGGCAGAGGGTGTGATCGAGGCGATACTCGATGGCATCGAGTCGACGCCATCGCCGTCACGCCCAGCCTCCAAGGCATAGCGCTGCTATCGAGGCCGACTCAGTACCTGTGGGAGCGGGCGTGCCCGCGAACACCGGCAAAGCCGGTGCCATCCATCGCGGTGGCTTCTTCGCGGGTAAACCC  >  NC_002947/81833‑82126

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑