Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,681,671 A→T 6.2% I64F (ATC→TTC)  recJ → ssDNA‑specific exonuclease RecJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,681,6710AT 6.2% 236.5 / 4.3 81I64F (ATC→TTC) recJssDNA‑specific exonuclease RecJ
Reads supporting (aligned to +/- strand):  ref base A (42/34);  new base T (3/2);  total (45/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.80e-01

TGGGTAACCTGCCACCCTTGCTGACCCGCCTTTACGCCGCGCGCGGCGTGCAGAGCGAGGCCGAGCTGGACAAGAGCCTGGCACGCCTGCTGCCCTACCAGCAGCTCAAGGGCATCGAGGCCGGTGTGGACCTGCTGGTCGAAGCCATCGACCAGCGCCAGCGCATCCTCATCGTCGGCGACTTCGATGCCGACGGTGCCACCGCCAGCACCGTCGGGGTGCTGGGCCTGCGCCTGCTGGGTGCGGCCCATGTCGATTACCTGGTGCCCAACCGCTTCGAGTATGGCTACGGCCT  >  NC_002947/1681525‑1681819
                                                                                                                                                  |                                                                                                                                                    
tGGGTAACCTGCCACCATTGCTGACCCGCCTTTACGGCGCGCGCGGCGTGCAGAGCGAGGCCGAGCTGGACAAGAGCCTGGCACGCCTGCTGCCCTACCAGCAGCTCAAGGGCATCGAGGCCGGTGTGGACCTGCTGGTCGAAGCCATc                                                                                                                                                    <  7:352098/149‑1 (MQ=255)
  ggTAACCTGCCACCCTTGCTGACCCGCCTTTACGCCGCGCGCGGCGTGCAGAGCGAGGCCGAGCTGGACAAGAGCCTGGCACGCCTGCTGCCCTACCAGCAGCTCAAGGGCATCGAGGCCGGTGTGGACCTGCTGGTCGAAGCCATCGa                                                                                                                                                  >  2:220557/1‑149 (MQ=255)
      aCCTGCCAGCCTTGCTGACCCGCCTTTAGGCCGCGCGCGGCGTGCAGAGCGAGGCCGAGCTGGACAAGAGCCTGGCACGCCTGCTGCCCTACCAGCAGCTCAAGGGCATCGAGGCCGGTGTGGACCTGCTGGTCGAAGCCATCGAcca                                                                                                                                               <  2:104646/148‑1 (MQ=255)
       ccTGCCAACCTTGCTGACCCGCCTTTACGCCGCGCGCGGCGTGCAGAGCGAGGCCGAGCTGGACAAGAGCCTGGCACGCCTGCTGCCCTACCAGCAGCTCAATGGCATCGAGGCCGGTGTGGACCTGCTGGTCGAAGCCATCGAccagc                                                                                                                                             <  3:218731/149‑1 (MQ=255)
        cTGCCACCCTTGCTGACCCGCCTTTACGCCGCGCGCGGCGTGCAGAGCGAGGCCGAGCTGGACAAGAGCCTGGCACGCCTGCTGCCCTACCAGCAGCTCAAGGGCATCGAGGCCGGTGTGGACCTGCTGGTCGAAGCCATCGAcca                                                                                                                                               >  8:182477/1‑146 (MQ=255)
        cTGCCACCCTTGCTGACCCGCCTTTACGCCGCGCGCGGCGTGCAGAGCGAGGCCGAGCTGGACAAGAGCCTGGCACGCCTGCTGCCCTACCAGCAGCTCAAGGGCATCGAGGCCGGTGTGGACCTGCTGGTCGAAGCCATCGAcca                                                                                                                                               <  7:182477/146‑1 (MQ=255)
         tGCCACCCTTGCTGACCCGCCTTTACGCCGCGCGCGGCGTGCAGAGCGAGGCCGAGCTGGACAAGAGCCTGGCACGCCTGCTGCCCTACCAGCAGCTCAAGGGCATCGAGGCCGGTGTGGACCTGCTGGTCGAAGCCATCGCccagcgc                                                                                                                                           >  6:13967/1‑149 (MQ=255)
           ccACGCTTGCTGACCCGCCTTTAGGCCGCGCGCGGCGTGCAGTGCGAGGCCGAGCTGGACAAGAGCCTGGCACGCCTGCTGCCCTACCAGCAGCTCAAGGGCATCGAGGCCGGTGTGGACCCGCTGGTCGATGGCTTCGAccagcag                                                                                                                                           <  4:112454/147‑3 (MQ=255)
           ccACCCTTGCTGACCCGCCTTTACGCCGCGCGCGGCGTGCAGAGCGAGGCCGAGCTGGACAAGAGCCTGGCACGCCTGCTGCCCTACCAGCAGCTCAAGGGCATCGAGGCCGGTGTGGACCTGCTGGTCGAAGCCATCGAccagcgc                                                                                                                                           <  8:341800/147‑1 (MQ=255)
           ccACCCTTGCTGACCCGCCTTTACGCCGCGCGCGGCGTGCAGAGCGAGGCCGAGCTGGACAAGAGCCTGGCACGCCTGCTGCCCTACCAGCAGCTCAAGGGCATCGAGGCCGGTGTGGACCTGCTGGTCGAAGCCATCGAccagcgc                                                                                                                                           >  7:341800/1‑147 (MQ=255)
                 ttGCTGACGCGCCTTTACGGCGCGCGCGGCGTGCAGATCGAGGCCGAGCTTGACAATAGCCTGGCACGCCTGCTGCCCTACCAGCAGCTCATGGGCATCGAGGCCGGTGTGGACCTGCTGGTCGAAGCCATCGACCAGCGCCATCGCAt                                                                                                                                   <  6:289043/149‑1 (MQ=255)
                   gCTGACCCGCCTTTACGCCGCGCGCGGCGTGCAGAGCGAGGCCGAGCTGGACAAGAGCCTGGCACGCCTGCTGCCCTACCAGCAGCTCAAGGGCATCGAGGCCGGTGTGGACCTGCTGGTCGAAGCCATCGACCAGCGCCAGCGCATc                                                                                                                                  <  2:337735/148‑1 (MQ=255)
                    cTGACCCGCCTTTACGCCGCGCGCGGCGTGCAGAGCGAGGCCGAGCTGGACAAGAGCCTGGCACGCCTGCTGCCCTACCAGCAGCTCAAGGGCATCGAGGCCGGTGTGGACCTGCTGGTCGAAGCCATCGACCAGCGCCAGCGCATCCt                                                                                                                                >  1:342679/1‑149 (MQ=255)
                           gCCTTTACGCCGCGCGCGGCGTGCAGAGCGAGGCCGAGCTGGACAAGAGCCTGGCACGCCTGCTGCCCTACCAGCAGCTCAAGGGCATCGAGGCCGGTGTGGACCTGCTGGTCGAAGCCATCGACCAGCGCCAGCGCATCCTCAtcgtc                                                                                                                         >  3:261478/1‑149 (MQ=255)
                            ccTTTACGCCGCGCGCGGCGTGCAGAGCGAGGCCGAGCTGGACAAGAGCCTGGCACGCCTGCTGCCCTACCAGCAGCTCAAGGGCATCGAGGCCGGTGTGGACCTGCTGGTCGAAGCCATCGACCAGCGCCAGCGCATCCTCAtcgtcg                                                                                                                        >  8:2184/1‑149 (MQ=255)
                             cTTTACGCCGCGCGCGGCGTGCAGAGCGAGGCCGAGCTGGACAAGAGCCTGGCACGCCTGCTGCCCTACCAGCAGCTCAAGGGCATCGAGGCCGGTGTGGACCTGCTGGTCGAAGCCATCGACCAGCGCCAGCGCATCCTCATCGTCgg                                                                                                                       <  1:38381/149‑1 (MQ=255)
                                  cgccgcGCGCGGCGTGCAGAGCGAGGCCGAGCTGGACAAGAGCCTGGCACGCCTGCTGCCCTACCAGCAGCTCAAGGGCATCGAGGCCGGTGTGGACCTGCTGGTCGAAGCCATCGACCAGCGCCAGCGCATCCTCATCGTCGGCGACt                                                                                                                  <  4:130190/149‑1 (MQ=255)
                                  cgccgcGCGCGGCGTGCAGAGCGAGGCCGAGCTGGACAAGAGCCTGGCACGCCTGCTGCCCTACCAGCAGCTCAAGGGCATCGACGCCGGTGTGGACCTGCTGGTCGAAGCCATCGACCAGCGCCAGCGCATCCTCATCGTCGGCAAc                                                                                                                   >  5:148118/1‑148 (MQ=255)
                                     cgcgcgcgGCGTGCAGAGCGAGGCCGAGCTGGACAAGAGCCTGGCACGCCTGCTGCCCTACCAGCAGCTCAAGGGCATCGAGGCCGGTGTGGACCTGCTGGTCGAAGCCATCGACCAGCGCCAGCGCATCCTCATCGTCGGCGACTTc                                                                                                                >  3:17325/1‑148 (MQ=255)
                                       cgcgcgGCGTGCAGAGCGAGGCCGAGCTGGACAAGAGCCTGGCACGCCTGCTGCCCTACCAGCAGCTCAAGGGCATCGAGGCCGGTGTGGACCTGCTGGTCGAAGCCATCGACCAGCGCCAGCGCATCCTCATCGTCGGCGACTTCGAt                                                                                                             <  1:220557/149‑1 (MQ=255)
                                        gcgcgGCGTGCAGAGCGAGGCCGAGCTGGACAAGAGCCTGGCACGCCTGCTGCCCTACCAGCAGCTCAAGGGCATCGAGGCCGGTGTGGACCTGCTGGTCGAAGCCATCGACCAGCGCCAGCGCATCCTCATCGTCGGCGACTTCGATg                                                                                                            <  2:373769/149‑1 (MQ=255)
                                          gcggcgTGCAGATCGAGGACGACCTGGACAAGAGCCTGGCACGCCTGCTGCCCTACCAGCAGCTCCAGGGCATCGAGGCCGGTGTGGACCTTCTGGTCGAAGCCATCGACCAGCGCCAGCGCATCCTCATCGTCGGCGACTTCGATGcc                                                                                                          <  2:114655/149‑1 (MQ=255)
                                                tGCAGAGCGAGGCCGAGCTGGACAAGAGCCTGGCACGCCTGCTGCCCTACCAGCAGCTCAAGGGCATCGAGGCCGGTGTGGACCTGCTGGTCGAAGCCATCGACCAGCGCCAGCGCATCCTCATCGTCGGCGACTTCGATGCCGACGGt                                                                                                    <  7:2184/149‑1 (MQ=255)
                                                 gCAGAGCGAGGCCGAGCTGGACAAGAGCCTGGCACGCCTGCTGCCCTACCAGCAGCTCAAGGGCATCGAGGCCGGTGTGGACCTGCTGGTCGAAGCCATCGACCAGCGCCAGCGCATCCTCATCGTCgg                                                                                                                       <  2:376685/129‑1 (MQ=255)
                                                 gCAGAGCGAGGCCGAGCTGGACAAGAGCCTGGCACGCCTGCTGCCCTACCAGCAGCTCAAGGGCATCGAGGCCGGTGTGGACCTGCTGGTCGAAGCCATCGACCAGCGCCAGCGCATCCTCATCGTCgg                                                                                                                       >  1:376685/1‑129 (MQ=255)
                                                 gCAGAGCGAGGCCGAGCTGGACAAGAGCCTGGCACGCCTGCTGCCCTACCAGCAGCTCAAGGGCATCGAGGCCGGAGTGGACCTGCTGGTCGAAGCCATCGACCAGCGCCAGCGCATCCTCATCGTCGGCGACTTCGATGCCGACGGTg                                                                                                   >  3:248246/1‑149 (MQ=255)
                                                   agagCGAGGCCGAGCTGGACAAGAGCCTGGCACGCCTGCTGCCCTACCAGCAGCTCAAGGGCATCGAGGCCGGTGTGGACCTGCTGGTCGAAGCCATCGACCAGCGCCAGCGCATCCTCATCGTCGGCGACTTCGATGCCGACGGTg                                                                                                   <  2:147866/147‑1 (MQ=255)
                                                   agagCGAGGCCGAGCTGGACAAGAGCCTGGCACGCCTGCTGCCCTACCAGCAGCTCAAGGGCATCGAGGCCGGTGTGGACCTGCTGGTCGAAGCCATCGACCAGCGCCAGCGCATCCTCATCGTCGGCGACTTCGATGCCGACGGTg                                                                                                   >  1:147866/1‑147 (MQ=255)
                                                   agagCGAGGCCGAGCTGGACAAGAGCCTGGCACGCCTGCTGCCCTACCAGCAGCTCAAGGGCATCGAGGCCGGTGTGGACCTGCTGGTCGAAGCCATCGACCAGCGCCAGCGCATCCTCATCGTCGGCGACTTCGATGCCGACGGTGc                                                                                                  >  6:70160/1‑148 (MQ=255)
                                                           gCCGAGCTGGACAAGAGCCTGGCACGCCTGCTGCCCTACCAGCAGCTCAAGGGCATCGAGGCCGGTGTGGACCTGCTGGTCGAAGCCATCGACCAGCGCCAGCGCATCCTCATCGTCGGCGACTTCGATGCCGACGGTGCCACCGCCAg                                                                                         >  5:67266/1‑149 (MQ=255)
                                                               aGCTGGACAAGAGCCTGGCACGCCTGCTGCCCTACCAGCAGCTCAAGGGCATCGAGGCCGGTGTGGACCTGCTGGTCGAAGCCATCGACCAGCGCCAGCGCATCCTCATCGTCGGCGACTTCGATGCCGACGGTGCCACCGCCAGCAcc                                                                                     <  8:226411/149‑1 (MQ=255)
                                                                gCTGGACAAGAGCCTGGCACGCCTGCTGCCCTACCAGCAGCTCAAGGGCATCGAGGCCGGTGTGGACCTGCTGGTCGAAGCCATCGACCAGCGCCAGCGCATCCTCATCGTCGGCGAGTTCGATGCCGACGGTGCCCCCGCCCGCCCCg                                                                                    >  6:252519/1‑149 (MQ=255)
                                                                  tGGACAAGAGCCTGGCACGCCTGCTGCCCTACCAGCAGCTCAAGGGCATCGAGGCCGGTGTGGACCTGCTGGTCGAAGCCATCGACCAGCGCCAGCGCATCCTCATCGTCGGCGACTTCGATGCCGACGGTGCCACCGCCAGCACCGTc                                                                                  >  7:116762/1‑149 (MQ=255)
                                                                    gACAAGAGCCTGGCACGCCTGCTGCCCTACCAGCAGCTCAAGGGCATCGAGGCCGCTGTGGACCTGCTGGTCGAAGCCATCGACCAGCGCCAGCGCATCCTCATCGTCGGCGACTTCGATGCCGACGGTGCCACCGCCAGCACCGTCgc                                                                                >  3:337496/1‑148 (MQ=255)
                                                                     aCAAGAGCCTGGCACGCCTGCTGCCCTACCAGCAGCTCAAGGGCATCGAGGCCGGTGTGGACCTGCTGGTCGAAGCCATCGACCAGCGCCAGCGCATCCTCATCGTCGGCGACTTCGATGCCGACGGTGCCACCGCCAGCACCGTCggg                                                                               >  1:314730/1‑149 (MQ=255)
                                                                     aCAAGAGCCGGGCAGGCCTGCTGCCCTACCAGCAGCTCAAGGGCATCGAGGCCGGTGTGGACCTGCTGGTCGAAGCCATCGACCAGCGCCAGCGCATCCTCATCGTCGGCGACTTCGATGCCGACGGTGCCACCGCCAGCACCGTCggg                                                                               <  5:252519/149‑1 (MQ=255)
                                                                       aaGAGCCTGGCACGCCTGCTGCCCTACCAGCAGCTCAAGGGCATCGAGGCCGGTGTGGACCTGCTGGTCGAAGCCATCGACCAGCGCCAGCGCATCCTCATCGTCGGCGACTTCGATGCCGACGGTGCCACCGCCAGCACCGTCGGGGt                                                                             >  5:234919/1‑149 (MQ=255)
                                                                          agCCTGGCACGCCTGCTGCCCTACCAGCAGCTCAAGGGCATCGAGGCCGGTGTGGACCTGCTGGTCGAAGCCATCGACCAGCGCCAGCGCATCCTCATCGTCGGCGACTTCGATGCCGACGGTGCCACCGCCAGCACCGTCGGGGTGCt                                                                          >  7:348146/1‑149 (MQ=255)
                                                                            ccTGGCACGCCTGCTGCCCTACCAGCAGCTCAAGGGCATCGAGGCCGGTGTGGACCTGCTGGTCGAAGCCATCGACCAGCGCCAGCGCATCCTCATCGTCGGCGACTTCGATGCCGACGGTGCCACCGCCAGCACCGTCGGGGTGCTgg                                                                        <  2:342679/149‑1 (MQ=255)
                                                                                  aCGCCTGCTGCCCTACCAGCAGCTCAAGGGCATCGAGGCCGGTGTGGACCTGCTGGTCGAAGCCATCGACCAGCGCCAGCGCATCCTCATCGTCGGCGACTTCGATGCCGACGGTGCCACCGCCAGCACCGTCGGGGTGCTGGgcctgc                                                                  >  7:63507/1‑149 (MQ=255)
                                                                                  aCGCCTGCTGCCCTACCAGCAGCTCAAGGGCATCGAGGCCGGTGTGGACCTGCTGGTCGAAGCCATCGACCAGCGCCAGCGCATCCTCATCGTCGGCGACTTCGATGCCGACGGTGCCACCGCCAGCACCGTCGGGGTGCTGGgcctgc                                                                  >  5:304829/1‑149 (MQ=255)
                                                                                   cGCCTGCTGCCCTACCAGCAGCTCAAGGGCATCGAGGCCGGTGTGGACCTGCTGGTCGAAGCCATCGACCAGCGCCAGCGCATCCTCATCGTCGGCGACTTCGATGCCGACGGTGCCACCGCCAGCACCGTCGGGGTGCTGGgcctgcg                                                                 <  4:17325/149‑1 (MQ=255)
                                                                                   cGCCTGCTGCCCTACCAGCAGCTCAAGGGCATCGAGGCCGGTGTGGACCTGCTGGTCGAAGCCATCGACCAGCGCCAGCGCATCCTCATCGTCGGCGACTTCGATGCCGACGGTGCCACCGCCAGCACCGTCGGGGTGCTGGgcctgcg                                                                 >  6:380628/1‑149 (MQ=255)
                                                                                      ctgctgCCCTACCAGCAGCTCAAGGGCATCGAGGCCGGTGTGGACCTGCTGGTCGAAGCCATCGACCAGCGCCAGCGCATCCTCATCGTCGGCGACTTCGATGCCGACGGTGACACCGCCAGCACCGTCGGGGTGCTGGgcctgcgcct                                                              >  4:166366/1‑149 (MQ=255)
                                                                                            cccTACCAGCAGCTCAAGGGCATCGAGGCCGGTGTGGACCTGCTGGTCGAAGCCATCGACCAGCGCCAGCGCATCCTCATCGTCGGCGACTTCGATGCCGACGGTGCCACCGCCAGCACCGTCGGGGTGCTGGGCCTGCGCCTGCTggg                                                        >  4:262103/1‑149 (MQ=255)
                                                                                              cTACCAGCAGCTCAAGGGCATCGAGGCCGGTGTGGACCTGCTGGTCGAAGCCATCGACCAGCGCCAGCGCATCCTCATCGTCGGCGACTTCGATGCCGACGGTGCCACCGCCAGCACCGTCGGGGTGCTGGGCCTGCGCCTGCTGGGTg                                                      >  3:39805/1‑149 (MQ=255)
                                                                                                 ccAGCAGCTCAAGGGCATCGAGGCCGGTGTGGACCTGCTGGTCGAAGCCATCGACCAGCGCCAGCGCATCCTCATCGTCGGCGACTTCGATGCCGACGGTGCCACCGCCAGCACCGTCGGGGTGCTGGGCCTGCGCCTGCTGGGTGCgg                                                   >  2:79504/1‑149 (MQ=255)
                                                                                                   agcagcTCAAGGGCATCGAGGCCGGGGTGGACCTGCTGGTCGAAGCCATCGACCAGCGCCAGCGCATCCTCATCGTCGGCGACTTCGATGCCGACGGTGCCACCGCCAGCACCGTCGGGGTGCTGGGCCTGCGCCTGCTGGGTGCGGcc                                                 <  8:63507/149‑1 (MQ=255)
                                                                                                    gcagcTCAAGGGCATCGAGGCCGGTGTGGACCTGCTGGTCGAAGCCATCGACCAGCGCCAGCGCATCCTCATCGTCGGCGACTTCGATGCCGACGGTGCCACCGCCa                                                                                          <  5:268104/107‑1 (MQ=255)
                                                                                                    gcagcTCAAGGGCATCGAGGCCGGTGTGGACCTGCTGGTCGAAGCCATCGACCAGCGCCAGCGCATCCTCATCGTCGGCGACTTCGATGCCGACGGTGCCACCGCCa                                                                                          >  6:268104/1‑107 (MQ=255)
                                                                                                        ataaaGGGCTTCGAGGCGGGTGTGGACCTGCTGGGCGAAGCCATCGACCTGCGCCTGCGAATCCTCATCGTCGGCGACTTCGATGCCGACGGTGCCACCGCCAGCACCGTCGGGGTGCTGGGCCTGCGCCTGCTGGGTGCGGCCCATGt                                            <  4:248246/146‑1 (MQ=255)
                                                                                                         tCAAGGGCATCGAGGCCGGTGTGGACCTGCTGGTCGAAGCCATCGACCAGCGCCAGCGCATCCTCATCGTCGGCGACTTCGATGCCGACGGTGCCACCGCCAGCACCGTCGGGGTGCTGGGCCTGCGCCTGCTGGGTGCGGCCCATGTc                                           >  8:159689/1‑149 (MQ=255)
                                                                                                           aaGGGCATCGAGGCCGGTGTGGACCTGCTGGTCGAAGCCATCGACCAGCGCCAGCGCATCCTCATCGTCGGCGACTTCGATGCCGACGGTGCCACCGCCAGCACCGTCGGGGTGCTGGGCCTGCGCCTGCTGGGTGCGGCCCATGTCGa                                         >  7:275360/1‑149 (MQ=255)
                                                                                                           aaGGGCATCGAGGCCGGTGTGGACCTGCTGGTCGAAGCCATCGACACGCGCCAGCGCATCCTCATCGTCGGCGACTTCGATGCCGACGGTGCCACCGCCAGCACCGTCGGGGTGCTGGGCCTGCGCCTGCTGGGTGCGGCCCATGTCGa                                         >  7:270363/1‑149 (MQ=255)
                                                                                                             gggCATCGAGGCCGGTGTGGCCCTGCTGGTCGAAGCCATCGACCAGCGCCAGCGCATCCTCATCGTCGGCGCCTTCGATGCCGACGGTGCCACCGCCAGCACCGTCGGGGTGCTGGGCCTGCGCCTGCTGGGTGCGGCCCATGTCGAt                                        >  6:110944/1‑148 (MQ=255)
                                                                                                             gggCATCGAGGCCGGTGTGGACCTGCTGGTCGAAGCCATCGACCAGCGCCAGCGCATCCTCATCGTCGGCGACTTCGATGCCGACGGTGCCACCGCCAGCACCGTCGGGGTGCTGGGCCTGCGCCTGCTGGGTGCGGCCCATGTCGAt                                        >  7:333867/1‑148 (MQ=255)
                                                                                                                cATCGAGGCCGGTGTGGACCTGCTGGTCGAAGCCATCGACCAGCGCCAGCGCATCCTCATCGTCGGCGACTTCGATGCCGACGGTGCCACCGCCAGCACCGTCTGGGTGCTGGGCCTGCGCCTGCTGGGTGCGGCCCATGTCGATTAcc                                    <  1:79504/149‑1 (MQ=255)
                                                                                                                 aTCGAGGCCGGTGTGGACCTGCTGGTCGAAGCCATCGACCAGCGCCAGCGCATCCTCATCGTCGGCGACTTCGATGCCGACGGTGCCACCGCCAGCACCGTCGGGGTGCTGGGCCTGCGCCTGCTGGGTGCGGCCCATGTCGATTACCt                                   >  8:102010/1‑149 (MQ=255)
                                                                                                                  tCGAGGCCGGTGTGGACCTGCTGGTCGAAGCCATCGACCAGCGCCAGCGCATCCTCATCGTCGGCGACTTCGATGCCGACGGTGCCACCGCCAGCACCGTCGGGGTGCTGGGCCTGCGCCTGCTGGGTGCGGCCCATGTCGATTACCTg                                  <  5:380628/149‑1 (MQ=255)
                                                                                                                  tCGAGGCCGGTGTGGACCTGCTGGTCGAAGCCATCGACCAGCGCCAGCGCATCCTCATCGTCGGCGACTTCGATGCCGACGGTGCCACCGCCAGCACCGTCGGGGTGCTGGGCCTGCGCCTGCTGGGTGCGGCCCATGTCGATTACCTg                                  <  6:67266/149‑1 (MQ=255)
                                                                                                                  tCGAGGCCGGTGTGGACCTGCTGGTCGAAGCCATCGACCAGCGCCAGCGCATCCTCATCGTCGGCGACTTCGATGCCGACGGTGCCACCGCCAGCACCGTCGGGGTGCTGGGCCTGCGCCTGCTGGGTGCGGCCCATGTCGATTACCTg                                  <  4:39805/149‑1 (MQ=255)
                                                                                                                   cGAGGCCGGTGTGGACCTGCTGGTCGAAGCCATCGACCAGCGCCAGCGCATCCTCAtc                                                                                                                            >  7:337168/1‑58 (MQ=255)
                                                                                                                   cGAGGCCGGTGTGGACCTGCTGGTCGAAGCCATCGACCAGCGCCAGCGCATCCTCAtc                                                                                                                            <  8:337168/58‑1 (MQ=255)
                                                                                                                   cGAGGCCGGTGTGGACCTGCTGGTCGAAGCCATCGACCAGCGCCAGCGCATCCTCATCGTCGGCGACTTCGATGCCGACGGTGCCACCGCCAGCACCGTCGGCGTCCTGGGCCTGCGCCTGCGGGGTGCGGCACAAGACGATTACCAgg                                 >  6:186175/1‑149 (MQ=255)
                                                                                                                         cGGTGTGGACCTGCTGGTCGAAGCCATCGACCAGCGCCAGCGCATCCTCATCGTCGGCGACTTCGATGCCGACGGTGCCACCGCCAGCACCGTCGGGGTGCTGGGCCTGCGCCTGCTGGGTGCGGCCCATGTCGATTACCTGGTGccc                            >  4:208307/1‑148 (MQ=255)
                                                                                                                                 tggcgctgGTCGATGGCTTCGACCAGCACCAGCGCATCCTCATCGTCGGCGACTTCGATGCCGACGGTGCCACCGCCAGCACCGTCGGGGTGCTGGGCCTGCGCCTGCTGGGTGCGGCCCATGTCGATTACCTGGTGCCCAACCGCTTc                   >  2:168583/5‑149 (MQ=255)
                                                                                                                                 aCCTGCTGGTCGAAGCCATCGACCAGCGCCAGCGCATCCTCATCGTCGGCGACTTCGATGCCGACGGTGCCACCGCCAGCACCGTCGGGGTGCTGGGCCTGCGCCTGCTGGGt                                                       >  3:349808/1‑113 (MQ=255)
                                                                                                                                 aCCTGCTGGTCGAAGCCATCGACCAGCGCCAGCGCATCCTCATCGTCGGCGACTTCGATGCCGACGGTGCCACCGCCAGCACCGTCGGGGTGCTGGGCCTGCGCCTGCTGGGt                                                       <  4:349808/113‑1 (MQ=255)
                                                                                                                                 aCCTGCTGGTCGAAGCCATCGACCAGCGCCAGCGCATCCTCATCGTCGGCGACTTCGATGCCGACGGTGCCACCGCCAGCACCGTCGGGGTGCTGGGCCTGCGCCTGCTGGGTGCGGCCCATGTCGATTACCTGGTGCCCAACCGCTTc                   >  6:319210/1‑149 (MQ=255)
                                                                                                                                  ccTGCTGGTCGAAGCCATCGACCAGCGCCAGCGCATCCTCATCGTCGGCGACTTCGATGCCGACGGTGCCACCGCCAGCACCGTCGGGGTGCTGGGCCTGCGCCTGCTGGGTGCGGCCCATGTCGATTACCTGGTGCCCAACCGCTTc                   >  4:211802/1‑148 (MQ=255)
                                                                                                                                   gcgctgGTCGATGGCTTCGACCAGCGCCAGCGCATCCTCATCGTCGGCGACTTCGATGCCGACGGTGCCACCGCCAGCACCGTCGGGGTGCTGGGCCTGCGCCTGCTgg                                                         >  1:353335/3‑109 (MQ=255)
                                                                                                                                   atgctgGTCGAAGCCATCGACCAGCGCCAGCGCATGCTCATCGTCGGTGTCTTCGATGGCGACGGTGCCACCGCCAGCACGGTCGGTGTGCTGGGCCTGAGCCTGCTGGGTGCTGCCGATGTCGATTACCTGGTTCCCAACCGCTTCGa                 <  4:337496/148‑1 (MQ=255)
                                                                                                                                     gctgGTCGATGGCTTCGACCAGCGCCAGCGCATCCTCATCGTCGGCGACTTCGATGCCGACGGTGCCACCGCCAGCACCGTCGGGGTGCTGGGCCTGCGCCTGCTGGGTGCGGCCCATGTCGATTACCTGGt                                >  1:237318/1‑132 (MQ=255)
                                                                                                                                     gctgGTCGATGGCTTCGACCAGCGCCAGCGCATCCTCATCGTCGGCGACTTCGATGCCGACGGTGCCACCGCCAGCACCGTCGGGGTGCTGGGCCTGCGCCTGCTGGGTGCGGCCCATGTCGATTACCTGGt                                <  2:237318/132‑1 (MQ=255)
                                                                                                                                       tggagttaGCCATCCACCAGGGCAAGCGCATCCTCATCTTCGGCGACGTCGAGGCCGACGGTGCCACCGCCAGCACCGTCGGGGTGCTGGGCCTGCGCCTGCTGGGTGCGTCCCATGTCGATTACCTGGTGCCCAACCGCTTCGAGTAt             <  8:247034/142‑1 (MQ=255)
                                                                                                                                       tgGTCGAAGGCAGCGACCAGCGCCAGGGCATCCTCATCGTGGGCGACTTCGATGCCGAGGGTGCCACCGCCAGCACCGTCGGGGTGCGGGGCCTGCGCCTGCTGGGTGCGGCCCATGTCGATTACCTGGTGCCCAACCGCTTCGAGTAt             <  5:186175/149‑1 (MQ=255)
                                                                                                                                         gTCGAAGCCATCGACCAGCGCCAGCGCATCCTCATCGTCGGCGACTTCGATGCCGACGGTGCCACCGCCAGCACCGTCGGGGTGCTGGGCCTGCGCCTGCTGGGTCCGGCCCATGTCGATTACCTGGTGCCCAACCGCTTCGAGTAt             <  3:262103/147‑1 (MQ=255)
                                                                                                                                            gAAGCCATCGACCAGCGCCAGCGCATCCTCATCGTCGGCGACTTCGATGCCGACGGTGCCACCGCCAGCACCGTCGGGGTGCTGGGCCTGCGCCTGCTGGGTGCGCCCCATGTCGATTACCTGGTGCCCAACCGCTTCGAGTATGGCt         <  6:304829/148‑1 (MQ=255)
                                                                                                                                               gCCATCGATCAGCGCCAGCGCATCCTCATCGTCGGCGACTTCGATGCCGACGGTGCCACCGCCAGCACCGTCGGGGTGCTGGGCCTGCGCCTGCTGGGTGCGGCCCATGTCGATTACCTGGTGCCCAACCGCTTCGAGTATGGCTACgg     >  2:296316/1‑149 (MQ=255)
                                                                                                                                               gCCATCGACCAGCGCCAGCGCATCCTCATCGTCGGCGACTTCGATGCCGACGGTGCCACCGCCAGCACCGTCGGGGTGCTGGGCCTGCGCCTGCTGGGTGCGGCCCATGTCGATTACCTGGTGCCCAACCGCTTCGAGTATGGCTACgg     >  6:109640/1‑149 (MQ=255)
                                                                                                                                                ccATCGACCAGCGCCAGCGCATCCTCATCGTCGGCGACTTCGATGCCGACGGTGCCACCGCCAGCACCGTCGGGGTGCTGGGCCTGCGCCTGCTGGGTGCGGCCCATGTCGATTACCTGGTGCCCAACCGCTTCGAGTATGGCTACGGc    <  5:13967/149‑1 (MQ=255)
                                                                                                                                                  aTCGACCAGCGCCAGCGCATCCTCATCGTCGGCGACTTCGATGCCGACGGTGCCACCGCCAGCACCGTCGGGGTGCTGGGCCTGCGCCTGCTGGGTGCGGCCCATGTCGATTACCTGGTGCCCAACCGCTTCGAGTATGGCTACGGCCt  >  6:303258/1‑149 (MQ=255)
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TGGGTAACCTGCCACCCTTGCTGACCCGCCTTTACGCCGCGCGCGGCGTGCAGAGCGAGGCCGAGCTGGACAAGAGCCTGGCACGCCTGCTGCCCTACCAGCAGCTCAAGGGCATCGAGGCCGGTGTGGACCTGCTGGTCGAAGCCATCGACCAGCGCCAGCGCATCCTCATCGTCGGCGACTTCGATGCCGACGGTGCCACCGCCAGCACCGTCGGGGTGCTGGGCCTGCGCCTGCTGGGTGCGGCCCATGTCGATTACCTGGTGCCCAACCGCTTCGAGTATGGCTACGGCCT  >  NC_002947/1681525‑1681819

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑