Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,704,624	G→C	11.9%	intergenic (+11/‑12)	PP_1498 → / → PP_1499	lipoprotein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,704,624	0	G	C	11.9%	179.9 / 10.4	65	intergenic (+11/‑12)	PP_1498/PP_1499	lipoprotein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (25/31);  new base C (2/6);  total (27/38)
Fisher's exact test for biased strand distribution p-value = 4.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTACCGAAGTGCCGGAGGACAACGGCGACGGCGTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAA‑ATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGCCCGC‑GAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTGACGCGGTGAA  >  NC_002947/1704477‑1704772                                                                                                                                                      |                                                                                                                                                       gtaCCGAAGTGCCGGAGGACAACGGCGACGGCGTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAA‑ATGACCG‑TGTTGGCGt                                                                                                                                                       <  8:327015/149‑1 (MQ=255)           gCCGGAGGACAACGGCGACGGCGTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAA‑ATGACCG‑TGTTGGCGTC‑TTCGCggg                                                                                                                                             <  2:120277/148‑1 (MQ=255)            ccGGAGGACAACGGCGACGGCGTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAACATGACCG‑TGTTGGCGTC‑TTCGCGGGc                                                                                                                                            <  1:3634/149‑1 (MQ=255)            ccGGAGGACAACGGCGACGGCGTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAA‑ATGACCG‑TGTTGGCGTC‑TTCGCGGGCt                                                                                                                                           >  5:367494/1‑149 (MQ=255)            ccGGAGGACAACGGCGACGGCGTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAA‑ATGACCG‑TGTTGGCGTC‑TTCGCGGGCt                                                                                                                                           >  5:175311/1‑149 (MQ=255)             cGGAGGACAACGGCGACGGCGTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAA‑ATGACCG‑TGTTGGCGTC‑TTCGCGGGCtt                                                                                                                                          >  1:126515/1‑149 (MQ=255)             cGGAGGACAACGGCGACGGCGTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAA‑ATGACCG‑TGTTGGCGTC‑TTCGCGGGCtt                                                                                                                                          <  3:67637/149‑1 (MQ=255)                 ggaCAACGGCGACGGCGTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAA‑ATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGccc                                                                                                                                      <  8:144392/149‑1 (MQ=255)                 ggaCAACGGCGACGGCGTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAA‑ATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGccc                                                                                                                                      <  8:203950/149‑1 (MQ=255)                   aCAACGGCGACGGCGTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAA‑ATGACCG‑TGTTGGCTTC‑TTCGCGGGCTTGCCcgc                                                                                                                                    <  2:262786/149‑1 (MQ=255)                    cAACGGCGACGGCGTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCGCGGGCAA‑ATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGCCcgc‑g                                                                                                                                  <  5:266230/149‑1 (MQ=255)                      ccggcgacggcgTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCGGTTCGTCGACGTGGCGCGGTTCCAGCGCTCGGGCAA‑ATGACCG‑TGTTGGCGTC‑TTCGCGGGCGTGCCCGC‑Gaa                                                                                                                                <  3:54732/148‑1 (MQ=255)                      acgtcgacggcgTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGTCCAACTCCGACCTGTTCGTCTAAGTGGCGCGGTTCCAGCGCTCGGGCAA‑ATGACCG‑TGTTGGCCTC‑TTCGCGGGCAAGCCCGC‑Gaa                                                                                                                                <  8:224785/149‑1 (MQ=255)                      acggcgacggcgTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAA‑ATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGCCCGC‑Gaa                                                                                                                                <  1:126176/149‑1 (MQ=255)                      acggcgacggcgTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAA‑ATGACCG‑TGTTGGCCTC‑TTCGCGGGCAAGCCCGC‑Gaa                                                                                                                                <  3:368556/149‑1 (MQ=255)                         gcgacggcaTTCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAA‑ATGACCG‑TGTTGGCCTC‑TTCGCGGGCAAGCCCGC‑GAagac                                                                                                                             <  1:132242/149‑2 (MQ=255)                                   gCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAA‑ATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGCCCGC‑GAAGAGGCCGGTACAg                                                                                                                   <  2:126515/149‑1 (MQ=255)                                    cTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAA‑ATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGCCCGC‑GAAGAGGCCGGTACAg                                                                                                                   <  5:149440/148‑1 (MQ=255)                                     tGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAA‑ATGACCG‑TGTCGGCCTC‑TTCGCGGGCAAGCCCGC‑GAAGACGcc                                                                                                                          >  8:252178/1‑140 (MQ=255)                                     tGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAA‑ATGACCG‑TGTCGGCCTC‑TTCGCGGGCAAGCCCGC‑GAAGACGcc                                                                                                                          <  7:252178/140‑1 (MQ=255)                                          gACTGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGTCCAACTCCGACCTGTTCGTCGAAGTGGCGCGTTTCCAGCGCGCGGGCAA‑ATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGCCCGC‑GAAGAGGCCGGTACAGGTTTCgt                                                                                                            <  7:305701/149‑1 (MQ=255)                                          gACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCTACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAA‑ATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGCCCGC‑GAAGAGGCCGGTACAGGTTTCgt                                                                                                            <  6:175311/149‑1 (MQ=255)                                           aCAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAA‑ATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGCCCGC‑GAAGAGGCCGGTACAGGTTTCgtg                                                                                                           >  8:154118/1‑149 (MQ=255)                                           aCAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAA‑ATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGCCCGC‑GAAGAGGCCGGTACAGGTTTCgtg                                                                                                           >  4:147824/1‑149 (MQ=255)                                             cgCCCGGCGCCGCCGCGGCTCCCCCACCGCGACCCCCCGCCCACCTCCCCCCGGTTCCTCCCCGCGGCGCGGTCCCACCCCTCGCCCAA‑CCGCCCG‑TGTGGGCCCC‑TCCGCGGGCTGGCCCGC‑GAAGAGGCCGGTACAGGTTTCGTGTa                                                                                                         <  4:227494/148‑1 (MQ=255)                                              gCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAA‑ATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGCCCGC‑GAAGAGGCCGGTACAGGTTTCGTGTAc                                                                                                        >  5:67694/1‑149 (MQ=255)                                                 cggcggCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAA‑ATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGCCCGC‑GAAGAGGCCGGTACAGGTTTCGTGTAc                                                                                                        >  1:240693/1‑146 (MQ=255)                                                  ggcggcGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAA‑ATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGCCCGC‑GAaga                                                                                                                              <  7:118383/123‑1 (MQ=255)                                                  ggcggcGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAA‑ATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGCCCGC‑GAaga                                                                                                                              >  8:118383/1‑123 (MQ=255)                                                    cggcgCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAA‑ATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGCCCGC‑GAAGAGGCCGGTACAGGTTTCGTGTACTAGaaa                                                                                                  <  3:31317/149‑1 (MQ=255)                                                      gcgcGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGGAA‑ATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGCCCGCTGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAg                                                                                                 <  1:243007/149‑1 (MQ=255)                                                       cgcgGGTGCTGGCGTACCTGGACGACCCGGCCAACTACGACGTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAA‑ATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGCCCGC‑GAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGa                                                                                               <  5:67253/149‑1 (MQ=255)                                                        gcgGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAA‑ATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGCCCGC‑GAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAg                                                                                              >  6:98599/1‑149 (MQ=255)                                                                tGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAA‑ATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGCCCGC‑CAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGAAGTTCCCGGt                                                                                      >  1:130480/1‑149 (MQ=255)                                                                  gCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAA‑ATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGCCCGC‑GAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTg                                                                                     <  7:154118/148‑1 (MQ=255)                                                                   ccTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAA‑ATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGCCCGC‑GAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGa                                                                                   <  6:367494/149‑1 (MQ=255)                                                                    cTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAA‑ATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGCCCGC‑GAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGAc                                                                                  >  4:101725/1‑149 (MQ=255)                                                                    cTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAA‑ATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGCCCGC‑GAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGAc                                                                                  <  3:236463/149‑1 (MQ=255)                                                                    cTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAA‑ATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGCCCGC‑GAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGAc                                                                                  >  2:57354/1‑149 (MQ=255)                                                                    cTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAA‑ATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGCCCGC‑GAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTACCGGTGGAc                                                                                  >  2:127291/1‑149 (MQ=255)                                                                    cTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAA‑ATGACCG‑TGTTGGCCTC‑TTCGCGGGCAAGCCCGC‑Gaa                                                                                                                                >  4:320602/1‑103 (MQ=255)                                                                    cTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAA‑ATGACCG‑TGTTGGCCTC‑TTCGCGGGCAAGCCCGC‑Gaa                                                                                                                                <  3:320602/103‑1 (MQ=255)                                                                         tGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAA‑ATGACCG‑TGTTGGCGTC‑TTCGCGGGGTTGGCCGC‑GAAGGGGCCGGTACAGGTTTCGTGGACTAGAAAGGAGTTCCCGGTGGCCGGAtg                                                                             >  4:225919/1‑148 (MQ=255)                                                                             cgacCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAA‑ATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGCCCGC‑GAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAg                                                                         <  5:98599/149‑1 (MQ=255)                                                                                 ccGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAA‑ATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGCCCGC‑GAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCaaa                                                                     <  3:147824/149‑1 (MQ=255)                                                                                 ccGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAA‑ATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGCCCGC‑GAAGAGGCCGGTAAAGGTTTCGGGTACTAGAAAGGAGTTCCCGGTGGACGAATACCCGCaaa                                                                     >  8:21810/1‑149 (MQ=255)                                                                                  cGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCCGTTCCAGCGCTCGGGCAA‑ATGACCG‑TGTTGGCGTC‑TACGCGGGCTTGCCCGC‑GAACAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCCGTGGACGAATACCAGCAAAc                                                                    >  3:72842/1‑149 (MQ=255)                                                                                    gCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAA‑ATGACCGATGTTGGCGTC‑TTCGCGGGCTTGCCCGC‑GAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCACGGTGGACGAATACCAGCAAAc                                                                    >  1:104037/1‑148 (MQ=255)                                                                                          tCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAA‑ATGACCG‑TGTTGGCGTC‑TTAGCCGGCGTGCCCGC‑GAACAGGCCCGTTAACGTTTAGTGTACATGAAAGGAGTTTCCGGGGGACCCATACAACCAAAcccaacccg                                                            >  2:164671/1‑142 (MQ=255)                                                                                               ccTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAA‑ATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGCCCGC‑GAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTg                                                       >  8:188023/1‑149 (MQ=255)                                                                                                   ttCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAA‑ATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGCCCGC‑GAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTcc                                                    <  8:40278/148‑1 (MQ=255)                                                                                                      gtcgAAGTGGCGCGGTTCCAGCGCTCGGGCAA‑ATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGCCCGC‑GAAGAGGc                                                                                                                           >  8:306221/1‑74 (MQ=255)                                                                                                      gtcgAAGTGGCGCGGTTCCAGCGCTCGGGCAA‑ATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGCCCGC‑GAAGAGGc                                                                                                                           <  7:306221/74‑1 (MQ=255)                                                                                                      gtcgAAGTGGCGCGGTTCCAGCGCTCGGGCAA‑ATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGCCCGC‑GAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGAc                                                 <  1:164671/148‑1 (MQ=255)                                                                                                       tcgAAGTGGCGCGGTTCCAGCGCTCGGGCAA‑ATGACCG‑TGTTGGCGTC‑TTACCGGGGGTTACCGG‑GAAGTGGGCCGTTCATGGTTATGGTACTAAAAAGGAGTTTCCCCGGGACGCAGAACAGCAAACACGCCACGGCCAGTCCGCCCg                                               >  2:179517/1‑149 (MQ=255)                                                                                                            gTGGCGCGGTTCCAGCGCTCGGGCAA‑ATGACCG‑TGTTGGCGTC‑TTCGCCGGCTTGCCCGC‑GAAGAGGCCGGTACAGGTTTCGTGTAATAGAAAGGAGTTACCGGTGGACGAAAACCAGCAAACCATACGCGCCCTGTCCGACCGCAtcg                                          >  8:40978/1‑149 (MQ=255)                                                                                                                 gcgGTTCCAGCGCTCGGGCAA‑ATGACCG‑TGTTGGCGTC‑TTCGCGGGCGTGCCCGC‑GCAGAGGCCCGGACACGTTTGGTGTAATAGAAAGGAGTTGCCGGTGGGCGGATAACAGCAAACCAAACGCGCCCGGTGCGACCGCCACGTcgcc                                     >  6:54068/1‑148 (MQ=255)                                                                                                                  cgGTTCCAGCGCTCGGGCAA‑ATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGCCCGC‑GAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCgg                                    <  6:67694/149‑1 (MQ=255)                                                                                                                    gTTCCAGCGCTCGGGCAA‑ATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGCCCGC‑GAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGca                                  >  7:92551/1‑149 (MQ=255)                                                                                                                     ttCCAGCGCTCGGGCAA‑ATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGCCCGC‑GAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGcac                                 <  3:290775/149‑1 (MQ=255)                                                                                                                        cAGCGCTCGGGCAA‑ATGACCG‑TGTTGGCGTCGTTCGCGGGCTTGCCCGC‑GAAGATGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATACGCGCCCTGTCCGACCGCATCGTCGCGGca                                  >  1:40802/1‑146 (MQ=255)                                                                                                                         aGCGCTCGGGCAA‑ATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGCCCGC‑GAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGAc                             <  3:101725/149‑1 (MQ=255)                                                                                                                                       cggcccG‑TGTTGGGGTC‑TTCGGCGGCTTGCCCGC‑GATGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATAGCAGCAAACCATCCGCGCCCTGTCCGAGCGCATCGTCGCGGCACAGACGCCGATCCGGGTc                <  4:72842/145‑1 (MQ=255)                                                                                                                                       aTGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGCCCGC‑GAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCAGAGACGCCGATCCGGGTc                <  2:104037/149‑1 (MQ=255)                                                                                                                                           ccG‑TGTTGGCGTC‑TTCGCGGGCTTGCCCGC‑GAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACTAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTg            <  4:139759/149‑1 (MQ=255)                                                                                                                                                      gTC‑TTCGCGGGCTTGCCCGC‑GAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTGACGCGGTGaa  <  8:92551/149‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                       GTACCGAAGTGCCGGAGGACAACGGCGACGGCGTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAA‑ATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGCCCGC‑GAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTGACGCGGTGAA  >  NC_002947/1704477‑1704772

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑