Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,704,637	T→A	12.7%	M1* (TTG→TAG) †‡	PP_1499 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,704,637	0	T	A	12.7%	165.6 / 13.2	69	M1* (TTG→TAG) †‡	PP_1499	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (27/32);  new base A (4/5);  total (31/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.35e-01

CGGAGGACAACGGCGACGGCGTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGCCCGC‑GAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCA‑CAGACGCCGATCCGGGTCCTTGACGCGGTGAAGTGGGAC  >  NC_002947/1704489‑1704779                                                                                                                                                       |                                                                                                                                                 cGGAGGACAACGGCGACGGCGTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCG‑TGTTGGCGTC‑TTCGCGGGCtt                                                                                                                                                  >  1:126515/1‑149 (MQ=255) cGGAGGACAACGGCGACGGCGTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCG‑TGTTGGCGTC‑TTCGCGGGCtt                                                                                                                                                  <  3:67637/149‑1 (MQ=255)     ggaCAACGGCGACGGCGTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGccc                                                                                                                                              <  8:144392/149‑1 (MQ=255)     ggaCAACGGCGACGGCGTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGccc                                                                                                                                              <  8:203950/149‑1 (MQ=255)       aCAACGGCGACGGCGTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCG‑TGTTGGCTTC‑TTCGCGGGCTTGCCcgc                                                                                                                                            <  2:262786/149‑1 (MQ=255)        cAACGGCGACGGCGTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCGCGGGCAAATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGCCcgc‑g                                                                                                                                          <  5:266230/149‑1 (MQ=255)          ccggcgacggcgTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCGGTTCGTCGACGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCG‑TGTTGGCGTC‑TTCGCGGGCGTGCCCGC‑Gaa                                                                                                                                        <  3:54732/148‑1 (MQ=255)          acgtcgacggcgTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGTCCAACTCCGACCTGTTCGTCTAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCG‑TGTTGGCCTC‑TTCGCGGGCAAGCCCGC‑Gaa                                                                                                                                        <  8:224785/149‑1 (MQ=255)          acggcgacggcgTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGCCCGC‑Gaa                                                                                                                                        <  1:126176/149‑1 (MQ=255)          acggcgacggcgTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCG‑TGTTGGCCTC‑TTCGCGGGCAAGCCCGC‑Gaa                                                                                                                                        <  3:368556/149‑1 (MQ=255)             gcgacggcaTTCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCG‑TGTTGGCCTC‑TTCGCGGGCAAGCCCGC‑GAagac                                                                                                                                     <  1:132242/149‑2 (MQ=255)                       gCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGCCCGC‑GAAGAGGCCGGTACAg                                                                                                                           <  2:126515/149‑1 (MQ=255)                        cTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGCCCGC‑GAAGAGGCCGGTACAg                                                                                                                           <  5:149440/148‑1 (MQ=255)                         tGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCG‑TGTCGGCCTC‑TTCGCGGGCAAGCCCGC‑GAAGACGcc                                                                                                                                  <  7:252178/140‑1 (MQ=255)                         tGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCG‑TGTCGGCCTC‑TTCGCGGGCAAGCCCGC‑GAAGACGcc                                                                                                                                  >  8:252178/1‑140 (MQ=255)                              gACTGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGTCCAACTCCGACCTGTTCGTCGAAGTGGCGCGTTTCCAGCGCGCGGGCAAATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGCCCGC‑GAAGAGGCCGGTACAGGTTTCgt                                                                                                                    <  7:305701/149‑1 (MQ=255)                              gACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCTACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGCCCGC‑GAAGAGGCCGGTACAGGTTTCgt                                                                                                                    <  6:175311/149‑1 (MQ=255)                               aCAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGCCCGC‑GAAGAGGCCGGTACAGGTTTCgtg                                                                                                                   >  8:154118/1‑149 (MQ=255)                               aCAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGCCCGC‑GAAGAGGCCGGTACAGGTTTCgtg                                                                                                                   >  4:147824/1‑149 (MQ=255)                                 cgCCCGGCGCCGCCGCGGCTCCCCCACCGCGACCCCCCGCCCACCTCCCCCCGGTTCCTCCCCGCGGCGCGGTCCCACCCCTCGCCCAACCGCCCG‑TGTGGGCCCC‑TCCGCGGGCTGGCCCGC‑GAAGAGGCCGGTACAGGTTTCGTGTa                                                                                                                 <  4:227494/148‑1 (MQ=255)                                  gCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGCCCGC‑GAAGAGGCCGGTACAGGTTTCGTGTAc                                                                                                                >  5:67694/1‑149 (MQ=255)                                     cggcggCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGCCCGC‑GAAGAGGCCGGTACAGGTTTCGTGTAc                                                                                                                >  1:240693/1‑146 (MQ=255)                                      ggcggcGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGCCCGC‑GAaga                                                                                                                                      >  8:118383/1‑123 (MQ=255)                                      ggcggcGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGCCCGC‑GAaga                                                                                                                                      <  7:118383/123‑1 (MQ=255)                                        cggcgCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGCCCGC‑GAAGAGGCCGGTACAGGTTTCGTGTACTAGaaa                                                                                                          <  3:31317/149‑1 (MQ=255)                                          gcgcGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGGAAATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGCCCGCTGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAg                                                                                                         <  1:243007/149‑1 (MQ=255)                                           cgcgGGTGCTGGCGTACCTGGACGACCCGGCCAACTACGACGTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGCCCGC‑GAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGa                                                                                                       <  5:67253/149‑1 (MQ=255)                                            gcgGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGCCCGC‑GAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAg                                                                                                      >  6:98599/1‑149 (MQ=255)                                                    tGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGCCCGC‑CAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGAAGTTCCCGGt                                                                                              >  1:130480/1‑149 (MQ=255)                                                      gCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGCCCGC‑GAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTg                                                                                             <  7:154118/148‑1 (MQ=255)                                                       ccTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGCCCGC‑GAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGa                                                                                           <  6:367494/149‑1 (MQ=255)                                                        cTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGCCCGC‑GAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGAc                                                                                          <  3:236463/149‑1 (MQ=255)                                                        cTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGCCCGC‑GAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGAc                                                                                          >  4:101725/1‑149 (MQ=255)                                                        cTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGCCCGC‑GAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGAc                                                                                          >  2:57354/1‑149 (MQ=255)                                                        cTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGCCCGC‑GAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTACCGGTGGAc                                                                                          >  2:127291/1‑149 (MQ=255)                                                        cTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCG‑TGTTGGCCTC‑TTCGCGGGCAAGCCCGC‑Gaa                                                                                                                                        >  4:320602/1‑103 (MQ=255)                                                        cTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCG‑TGTTGGCCTC‑TTCGCGGGCAAGCCCGC‑Gaa                                                                                                                                        <  3:320602/103‑1 (MQ=255)                                                             tGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCG‑TGTTGGCGTC‑TTCGCGGGGTTGGCCGC‑GAAGGGGCCGGTACAGGTTTCGTGGACTAGAAAGGAGTTCCCGGTGGCCGGAtg                                                                                     >  4:225919/1‑148 (MQ=255)                                                                 cgacCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGCCCGC‑GAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAg                                                                                 <  5:98599/149‑1 (MQ=255)                                                                     ccGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGCCCGC‑GAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCaaa                                                                             <  3:147824/149‑1 (MQ=255)                                                                     ccGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGCCCGC‑GAAGAGGCCGGTAAAGGTTTCGGGTACTAGAAAGGAGTTCCCGGTGGACGAATACCCGCaaa                                                                             >  8:21810/1‑149 (MQ=255)                                                                      cGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCCGTTCCAGCGCTCGGGCAAATGACCG‑TGTTGGCGTC‑TACGCGGGCTTGCCCGC‑GAACAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCCGTGGACGAATACCAGCAAAc                                                                            >  3:72842/1‑149 (MQ=255)                                                                        gCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGATGTTGGCGTC‑TTCGCGGGCTTGCCCGC‑GAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCACGGTGGACGAATACCAGCAAAc                                                                            >  1:104037/1‑148 (MQ=255)                                                                              tCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCG‑TGTTGGCGTC‑TTAGCCGGCGTGCCCGC‑GAACAGGCCCGTTAACGTTTAGTGTACATGAAAGGAGTTTCCGGGGGACCCATACAACCAAAcccaacccg                                                                    >  2:164671/1‑142 (MQ=255)                                                                                   ccTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGCCCGC‑GAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTg                                                               >  8:188023/1‑149 (MQ=255)                                                                                       ttCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGCCCGC‑GAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTcc                                                            <  8:40278/148‑1 (MQ=255)                                                                                          gtcgAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGCCCGC‑GAAGAGGc                                                                                                                                   <  7:306221/74‑1 (MQ=255)                                                                                          gtcgAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGCCCGC‑GAAGAGGc                                                                                                                                   >  8:306221/1‑74 (MQ=255)                                                                                          gtcgAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGCCCGC‑GAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGAc                                                         <  1:164671/148‑1 (MQ=255)                                                                                           tcgAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCG‑TGTTGGCGTC‑TTACCGGGGGTTACCGG‑GAAGTGGGCCGTTCATGGTTATGGTACTAAAAAGGAGTTTCCCCGGGACGCAGAACAGCAAACACGCCACGGCCAGTCCGCCCg                                                       >  2:179517/1‑149 (MQ=255)                                                                                                gTGGCGCGGTTCCAGCGCTCGGGCAAATGACCG‑TGTTGGCGTC‑TTCGCCGGCTTGCCCGC‑GAAGAGGCCGGTACAGGTTTCGTGTAATAGAAAGGAGTTACCGGTGGACGAAAACCAGCAAACCATACGCGCCCTGTCCGACCGCAtcg                                                  >  8:40978/1‑149 (MQ=255)                                                                                                     gcgGTTCCAGCGCTCGGGCAAATGACCG‑TGTTGGCGTC‑TTCGCGGGCGTGCCCGC‑GCAGAGGCCCGGACACGTTTGGTGTAATAGAAAGGAGTTGCCGGTGGGCGGATAACAGCAAACCAAACGCGCCCGGTGCGACCGCCACGTcgcc                                             >  6:54068/1‑148 (MQ=255)                                                                                                      cgGTTCCAGCGCTCGGGCAAATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGCCCGC‑GAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCgg                                            <  6:67694/149‑1 (MQ=255)                                                                                                        gTTCCAGCGCTCGGGCAAATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGCCCGC‑GAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGca                                          >  7:92551/1‑149 (MQ=255)                                                                                                         ttCCAGCGCTCGGGCAAATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGCCCGC‑GAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGca‑c                                        <  3:290775/149‑1 (MQ=255)                                                                                                            cAGCGCTCGGGCAAATGACCG‑TGTTGGCGTCGTTCGCGGGCTTGCCCGC‑GAAGATGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATACGCGCCCTGTCCGACCGCATCGTCGCGGca                                          >  1:40802/1‑146 (MQ=255)                                                                                                             aGCGCTCGGGCAAATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGCCCGC‑GAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCA‑CAGAc                                    <  3:101725/149‑1 (MQ=255)                                                                                                                          cggcccG‑TGTTGGGGTC‑TTCGGCGGCTTGCCCGC‑GATGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATAGCAGCAAACCATCCGCGCCCTGTCCGAGCGCATCGTCGCGGCA‑CAGACGCCGATCCGGGTc                       <  4:72842/145‑1 (MQ=255)                                                                                                                          aTGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGCCCGC‑GAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCA‑GAGACGCCGATCCGGGTc                       <  2:104037/149‑1 (MQ=255)                                                                                                                              ccG‑TGTTGGCGTC‑TTCGCGGGCTTGCCCGC‑GAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACTAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCA‑CAGACGCCGATCCGGGTCCTTg                   <  4:139759/149‑1 (MQ=255)                                                                                                                                         gTC‑TTCGCGGGCTTGCCCGC‑GAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCA‑CAGACGCCGATCCGGGTCCTTGACGCGGTGaa         <  8:92551/149‑1 (MQ=255)                                                                                                                                          tC‑TTCGCGGGCAAGCCCGC‑GAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACAAGCAAACCATCCGCGCACTGTCCGACAGCATCGTCGCGGCA‑CAGACGACGATCCGGGTCCTTGACGCGGTGAAg        >  1:24640/1‑149 (MQ=255)                                                                                                                                             ttCGCGGGCAAGCCCGC‑GAAGACGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCA‑CAGACGCCGATCCGGGTCCTTGACGCGGTGAAGTg      >  2:244197/1‑149 (MQ=255)                                                                                                                                              tCGCGGGCTTGCCCGC‑GAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCA‑CAGAc                                    >  2:319379/1‑118 (MQ=255)                                                                                                                                              tCGCGGGCTTGCCCGC‑GAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCA‑CAGAc                                    <  1:319379/118‑1 (MQ=255)                                                                                                                                              tCGCGGGCTTGCCCGC‑GAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCA‑CAGACGCCGATCCGGGTCCTTGACGCGGTGAAGTgg     >  2:191207/1‑149 (MQ=255)                                                                                                                                               cgcgGGCTTGCCCGC‑GAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACCAGACGCCGATCCGGGTCCTTGACGCGGTGAAGTg      <  1:57354/148‑1 (MQ=255)                                                                                                                                               cgcgGGCTTGCCCGC‑GAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCA‑CAGACGCCGATCCGGGTCCTTGACGCGGTGAAGTgg     <  7:21810/148‑1 (MQ=255)                                                                                                                                               cgcgGGCTTGCCCGC‑GAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCA‑CAGACGCCGATCCGGGTCCTTGACGCGGTGAAGTgg     >  5:353975/1‑148 (MQ=255)                                                                                                                                                gcggtattGCCCGC‑GAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCGGGTGGACTAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCA‑CAGACGCCGATGCGGGTCCTTGACGCGGTGAAGTGGga   <  2:130480/143‑1 (MQ=255)                                                                                                                                                gcgGGCTTGCCCGC‑GAAGAGGCCGGGACAGGTGTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCA‑CAGCCGCGGATCCGGGTCCTGGACGCGATGAAGTgggc   >  6:92497/1‑148 (MQ=255)                                                                                                                                                  gggCTTGCCCGC‑GAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCA‑CAGACGCCGATCCGGGTCCTTGACGCGGTGAAGTGGgac  >  4:198819/1‑148 (MQ=255)                                                                                                                                                       |                                                                                                                                                 CGGAGGACAACGGCGACGGCGTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCG‑TGTTGGCGTC‑TTCGCGGGCTTGCCCGC‑GAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCA‑CAGACGCCGATCCGGGTCCTTGACGCGGTGAAGTGGGAC  >  NC_002947/1704489‑1704779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑