Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,751,081 C→A 17.0% S147* (TCG→TAG) ‡ PP_1551 → replication protein O

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,751,0810CA17.0% 75.8 / 9.1 42S147* (TCG→TAG) ‡PP_1551replication protein O
Reads supporting (aligned to +/- strand):  ref base C (8/23);  new base A (5/2);  total (17/25)
Fisher's exact test for biased strand distribution p-value = 3.41e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.16e-01

GACTGGATTTACGTTGATCCGAAACAGACCAAAACAGCCGACTCGGCTGAAGTGGTCCGAATCGGCTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTT‑CGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCAAAGCCGCCATGCTGGCCGACAATCCGCACGGGCTCGATGAATCCCTGATCGCTGATTACTTGGCTGTCCGCAA  >  NC_002947/1750939‑1751228
                                                                                                                                                |                                                                                                                                                   
gACTGGATTTACGTTGATCCGAAACAGACCAAAACAGCCGACTCGGCTGAAGTGGTCCGAATCGGCTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTT‑CGGAAGa                                                                                                                                               <  1:5495/149‑1 (MQ=255)
 aCTGGATTTACGTTGATCCGAAACAGACCAAAACAGCCGACTCGGCTGAAGTGGTCCGAATCGGCTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAC‑ATCTTCCGA‑AGGAAGAt                                                                                                                                              <  1:281977/149‑1 (MQ=255)
   tGGATTTACGTTGATCCGAAACAGACCAAAACAGCCGACTCGGCTGAAGTGGTCCGAATCGGCTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTACGT‑CGGAAGATGt                                                                                                                                            >  1:202912/1‑149 (MQ=255)
     gATTTACGTTGATCCGAAACAGACCAAAACAGCCGACTCGGCTGAAGTGGTCCGAATCGGCTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATCCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTT‑CGGAAGATGtt                                                                                                                                           <  1:267668/148‑1 (MQ=255)
       ggtccGTTGATCCGACACAGACCAAAACAGCCGACTCGGCTGAAGTTGTCCGAATCGTCTCAGAGTCGAACCAGACCAAAACCGCCGACTCCCTTCTTTATTCTCAGAAAGACACCCCCTATGTAA‑ATCTTCCTT‑CGGAAGAT‑TTACAt                                                                                                                                       <  5:294009/145‑1 (MQ=255)
                 tCCGAAACAGACCAAAACAGCCGACTCGGCTGAAGTGGTCCGAATCGGCTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAGCCCCCCAATGTAA‑ATCTTGCGT‑GGGGAGATGGTACATGCCCCCCCa                                                                                                                              >  8:348167/1‑149 (MQ=255)
                  ccGAAACAGACCAAAACAGCCGACTCGGCTGAAGTGGTCCGAATCGGCTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCGCCTCTGGAA‑ATCTGGCTG‑GGGAAGATGGTTCATGAGACCCCAg                                                                                                                             >  6:134177/1‑149 (MQ=255)
                    gAAACAGACCAAAACAGCCGACTCGGCTGAAGTGGTCCGAATCGGCTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTT‑CGGAAGATGTTACATGCCCCCCCAGCg                                                                                                                           <  5:321918/149‑1 (MQ=255)
                          gACCAAAACAGCCGACTCGGCTGAAGTGGTCCGAATCGGCTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTTCCGGAAGATGTTACATGCCCCCCCAGCgagccg                                                                                                                      <  1:45561/149‑1 (MQ=255)
                             cAAAACAGCCGACTCGGCTGAAGTGGTCCGAATCGGCTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGACACGGCCTAGGTTA‑ATCTTCCTG‑CGTAAGATGTTACATGGCCCGCCAGCgcgccggcgc                                                                                                                  >  8:158715/1‑149 (MQ=255)
                             cAAAACAGCCGACTCGGCTGAAGTGGTCCGAATCGGCTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCGATGTAA‑ATCTTACTG‑CGGAAGATGTTACATTCACACCCAGCgagccggcgc                                                                                                                  >  2:139830/1‑149 (MQ=255)
                             aaaaaCCGCAGAGTCGGCTTAAGTGTTCAGAATCGGCTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAATAAAGAAACCCCCTATGTAA‑ATCTTCCTT‑CGGAAGATGTTACATGCCCCCCCAGCgagccggagc                                                                                                                  <  8:302742/148‑1 (MQ=255)
                                       gACTCGGCTGAAGTGGTCCGAATCGGCTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATATTGCTT‑CGGAAGATGGTAGATGACCACCCACCGCGCCCGCGCCGACGCCCGc                                                                                                        >  2:224652/1‑149 (MQ=255)
                                            ggCTGAAGTGGTCCTAATCGGCTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTT‑CGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAgg                                                                                                   <  3:216052/149‑1 (MQ=255)
                                            ggCTGAAGTGGTCCGAATCGGCTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTT‑CGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAg                                                                                                    <  5:263798/148‑1 (MQ=255)
                                              cTGAAGTGGTCCGAATCGGCTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTT‑CGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAg                                                                                                    <  1:62498/146‑1 (MQ=255)
                                              cTGAAGTGGTCCGAATCGGCTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTT‑CGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAGGAt                                                                                                 <  3:312705/149‑1 (MQ=255)
                                                 atttgttCCGAATCGGATCAGAGTCTAAACATACAAAAACTGCAGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTT‑CGGAAGATGTTACATGCCCACCCAGCGAGCCGGAGCCGACTCCGGCCAAGGATGa                                                                                               <  8:337732/142‑1 (MQ=255)
                                                 aaGTGGTCCGAATCGGCTATGAGTAGAAACAGTCCAAAAACGCCGACTCCCTTCTTTATTCTAATAAAGAAACCCCCAATGTAA‑ATATTCCTT‑CGGAATATGTTACATGCCCACCCAGCGAGCCGGAGCCGACTCCGGCCAAGGATGa                                                                                               <  6:35453/148‑1 (MQ=255)
                                                                    agagTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCAAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTT‑CGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCgg                                                                             <  7:348167/147‑1 (MQ=255)
                                                                    agagTCGAAACACACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTT‑CGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCgg                                                                             <  4:262994/147‑1 (MQ=255)
                                                                      agTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTT‑CGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCaaa                                                                         <  5:296310/149‑1 (MQ=255)
                                                                       gTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTT‑CGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCaaa                                                                         <  5:134177/148‑1 (MQ=255)
                                                                             aCAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTT‑CGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCAAAGCCGCCa                                                                  <  6:187210/149‑1 (MQ=255)
                                                                                  ccAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAGCGCCCGATGTAA‑ATCTTGGTT‑GGGGAGATGTTACATGAGGCCCAAGCGAGGCGGGGCCGCGACCGGCGAAGGAGGAGCGCAAGGCGACCAACGGGAAAgccgcacagcgg                                                             >  4:177597/1‑142 (MQ=255)
                                                                                   cAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAGGCGCCCGATGTAA‑ATCTTACTT‑CGGCAGATGTTAGATGCACCCCCAGCGACCCGGAGCCGACGCCGGCCAAGCAGGCGCGCAAGGCGCACTGCGCCCAAGCCGCCAAGCTgg                                                            >  4:203708/1‑149 (MQ=255)
                                                                                            cgACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTT‑CGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCAAAGCCGCCATGCTGGCCGACAATc                                                   <  7:158715/149‑1 (MQ=255)
                                                                                                  ccTTCTTTATTCTAAGAAAGAGGCGCCCTATGTAA‑ATCTTCATT‑CGGAAGAGGTTACATGACCCCCCGGCGACCCGCCGCCGACACCGGCCCAGGAGGAGCGGCAGGCGCCCATCGGCAAAGCCGCCAGGCTGGCCCGCAAGCCGCCCg                                             >  2:124402/1‑149 (MQ=255)
                                                                                                  ccTTCTTTATTCTAAGAAAGAACCCCCCTATGTAA‑ATCTTGCTT‑AGGAAGATGTTACAAGCCAACACAGCGAGCCGGAGCCGACTACGGCCAAGGGAGACCGAAAGGCACCCTACGGCAAACACCCCATGCAGGCCGACAAACCGCAcc                                             >  6:381288/1‑148 (MQ=255)
                                                                                                  ccTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTT‑CGGAAGAt                                                                                                                                              <  4:362277/52‑1 (MQ=255)
                                                                                                  ccTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTT‑CGGAAGAt                                                                                                                                              >  3:362277/1‑52 (MQ=255)
                                                                                                              tAAGAAAGAAGCCCCCTATGTAA‑ATCTTCCGT‑GGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAGGATGAGCGGAAGGCGCCCTTCGGCGAAGCCGCCACGCAGGCCGACCATCCGCACGGGCGCGaggcat                                 >  8:285810/1‑144 (MQ=255)
                                                                                                                        aCCCCCTATGTAA‑ATCTTCCTT‑CGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCAAAGCCGCCATGCTGGCCGACAATCCGCACGGGCTCGATGAATCCCTGATCGc                       <  3:177597/149‑1 (MQ=255)
                                                                                                                            ccTATGTAA‑ATCTTCCTT‑CGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCAAAGCCGCCATGCTGGCCGACAATCCGCACGGGCTCGATGAATCCCTGATCGCTGAt                   >  5:211033/1‑149 (MQ=255)
                                                                                                                               aTGTAACATCTTCCGA‑AGGAAGAT‑TTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCAAAGCCGCCATGCTGGCCGACAATCCGCACGGGCTCGATGAATCCCTGATCGCTGATTAc                >  5:124671/1‑149 (MQ=255)
                                                                                                                                tgtaacaTCTTCCGA‑AGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCAAAGCCGCCATGCTGGCCGACAATCCGCACGGGCTCGATGAATCCCTGATCGCTg                     >  8:286275/7‑144 (MQ=255)
                                                                                                                                tgtaacaTCTTCCGA‑AGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCAAAGCCGCCATGCTGGCCGACAATCCGCACGGGCTCGATGAATCCCTGATCGCTg                     <  7:286275/138‑1 (MQ=255)
                                                                                                                                 gtaacaTCTTCCGA‑AGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCAAAGCCGCCATGCTGGCCGACAATCCGCACGGGCTCGATGAATCCCTGATCGCTGATTACt               >  5:195193/6‑149 (MQ=255)
                                                                                                                                    a‑aTCTTCGTT‑CGGTAGATGTTACTTGGCCCCCCAGGGAGCGGGAGGCGAGTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGGAAAGCGGCCATGCTGGCCGACAATCCGCACGGGCTCGATGAATCCCTGATCGCTGATTACTTgg            <  5:307229/148‑1 (MQ=255)
                                                                                                                                    a‑aTCTTCCTT‑CGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCAAAGCCGCCATGCTGGCCGACAATCCGCACGGGCTCGATGAATCCCTGATCGCTGATTACTTgg            <  2:202912/148‑1 (MQ=255)
                                                                                                                                     caTCTTCCGA‑AGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCAAAGCCGCCATGCTGGCCGACAATCCGCACGGGCTCGATGAATCCCTGATCGCTGATTACTTGGc           >  8:351939/2‑149 (MQ=255)
                                                                                                                                      aTCTTCCTT‑CGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCAAAGCCGCCATGCTGGCCGACAATCCGCACGGGCTCGATGAATCCCTGATCGCTGATTACTTGGCt          <  6:211033/149‑1 (MQ=255)
                                                                                                                                           cctt‑cGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGCCTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCAAAGCCGCCATGCTGGCCGACAATCCGCACGGGCTCGATGAATCCCGGATCGCTGATTACTTGGCTGTCCg     >  3:42284/1‑149 (MQ=255)
                                                                                                                                           cctt‑cGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCAAAGCCGCCATGCTGGCCGACAATCCGCACGGGCTCGATGAATCCCTGATCGCTGa                    >  2:162726/1‑134 (MQ=255)
                                                                                                                                           cctt‑cGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCAAAGCCGCCATGCTGGCCGACAATCCGCACGGGCTCGATGAATCCCTGATCGCTGa                    <  1:162726/134‑1 (MQ=255)
                                                                                                                                           cctt‑cGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCAAAGCCGCCATGCTGGCCGACAATCCGCACGGGCTCGATGAATCCCTGATCGCTGATTACTTGGCTGTCCg     >  3:46499/1‑149 (MQ=255)
                                                                                                                                             tt‑cGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCAAAGCCGCCATGCTGGCCGACAATCCGCACGGGCTCGATGAATCCCTGATCGCTGATTACTTGGCTGTCCGc    >  8:208918/1‑148 (MQ=255)
                                                                                                                                                cGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCAAAGCCGCCATGCTGGCCGACAATCCGCACGGGCTCGATGAATCCCTGATCGCTGATTACTTGGCTGTCCGCaa  <  1:139830/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
GACTGGATTTACGTTGATCCGAAACAGACCAAAACAGCCGACTCGGCTGAAGTGGTCCGAATCGGCTCAGAGTCGAAACAGACCAAAACCGCCGACTCCCTTCTTTATTCTAAGAAAGAAACCCCCTATGTAA‑ATCTTCCTT‑CGGAAGATGTTACATGCCCCCCCAGCGAGCCGGAGCCGACTCCGGCCAAGGATGAGCGCAAGGCTCCCTTCGGCAAAGCCGCCATGCTGGCCGACAATCCGCACGGGCTCGATGAATCCCTGATCGCTGATTACTTGGCTGTCCGCAA  >  NC_002947/1750939‑1751228

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑