Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,795,404	A→G	5.4%	I73V (ATC→GTC)	PP_1600 →	Skp‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,795,404	0	A	G	5.4%	221.2 / 2.0	74	I73V (ATC→GTC)	PP_1600	Skp‑like protein
Reads supporting (aligned to +/- strand):  ref base A (39/31);  new base G (2/2);  total (41/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

AAGGTTGCCGTCCTGAACTACCAGATGGCCCTGCTCGAGTCGGATGCCGCCAAGAAGTATGCGGTTGA‑TGCCGAGAAGAAATTCGGTCCGCAACTGACCAAGC‑TGAAAAGCCTGGAAAGCAGTGCCAAGGGCATCCAGGACCGCCTGATCAAGGGCG‑GCGACAAGATGCAGCAGCAGGAGCGTGAGCGCCTGGAGCTCGAGTTCAAGCAAAAGGCCCGCGATTTCCAGTTCCAGTCCAAGGAACTGAACGAAGCCAAGGCCGTTGCTGATCGCGACATGCTCAAGCAACTGAAGC  >  NC_002947/1795257‑1795551                                                                                                                                                      |                                                                                                                                                     aaGGTTGCCGTCCTGAACTACCAGATGGCCCTGCTCGAGTCGGATGCCGCCAAGAAGTATGCGGTTGA‑TGCCGAGAAGAAATTCGGTCCGCAACTGACCAAGC‑TGAAAAGCCTGGAAAGCAGTGCCAAGGGCATCCAGGACCGCCTGa                                                                                                                                                      <  4:290054/148‑1 (MQ=255)    gTTGCCGTCCTGAACTACCAGATGGCCCTGCTCGAGTCGGATGCCGCCAAGAAGTATGCGGTTGAGTCCCGAGAAGAAATACGGACCGAAACTCACAAACA‑TGAAAAGCGTGAAAAGCAGTGCAATGGCCATCAAGGACCGCATGAta                                                                                                                                                    >  1:114403/1‑147 (MQ=255)           tCCTGAACTACCAGATGGCCCTGCTCGAGTCGGATGCCGCCAAGAAGTATGCGGTTGA‑TGCCGAGAAGAAATTCGGTCCGCAACTGACCAAGC‑TGAAAAGCCTGGAAAGCAGTGCCAAGGGCATCCAGGACCGCCTGATCAAGggcg‑gc                                                                                                                                          <  2:207259/149‑1 (MQ=255)                aaCTACCAGATGGCCCTGCTCGAGTCGGATGCCGCCAAGAAGTATGCGGTTGA‑TGCCGAGAAGAAATTCGGGCCGCAACTGACCAAGC‑TGAAAAGCCTGGAAAGCAGTGCCAAGGGCATCCAGGACCGCCTGATCAAGGGCG‑GCGAc                                                                                                                                       <  3:248618/147‑1 (MQ=255)                   tACCAGATGGCCCTGCTCGAGTCGGATGCCGCCAAGAAGTATGCGGTTGA‑TGCCGAGAAGAAATTCGGTCCGCAACTGACCAAGC‑TGAAAAGCCTGGAAAGCAGTGCCAAGGGCATCCAGGACCGCCTGATCAAGGGCG‑GCGACAAGAt                                                                                                                                  >  4:7814/1‑149 (MQ=255)                     ccAGATGGCCCTGCTCGAGTCGGATGCCGCCAAGAAGTATGCGGTTGA‑TGCCGAGAAGAAATTCGGTCCGCAACTGACCAAGC‑TGAAAAGCCTGGTAAGCAGTGCCAAGGGCATCCAGGACCGCCTGATCAAGGGCG‑GCGACAAGATg                                                                                                                                 >  1:310129/1‑148 (MQ=255)                           ggCCCTGCTCGAGTCGGATGCCGCGAAGAAGTATGCGGGTGA‑GGCCGAGAAGAAATTCGGTCCGCAACTGACCAAGC‑TGAAAAGCCTGGAAAGCAGTGCCAAGGGCATCCAGGACCGCCTGATCAAGGGCG‑GCGACAAGATgcagcag                                                                                                                           <  8:330728/148‑1 (MQ=255)                            gCCCTGCTCGAGTCGGATGCCGCCAAGAAGTATGCGGTTGA‑TGCCGAGAAGAAATTCGGTCCGCAACTGACCAAGC‑TGAAAAGCCTGGAAAGCAGTGCCAAGGGCATCCAGGACCGCCTGATCAAGGGCG‑GCGACAAGATgcagcag                                                                                                                           >  1:304486/1‑147 (MQ=255)                              ccTGCTCGAGTCGGATGCCGCCAAGAAGTATGCGGTTGA‑TGCCGAGAAGAAATTCGGTCCGCAACTGACCAAGC‑TGAAAAGCCTGGAAAGCAGTGCCAAGGGCATCCAGGACCGCCTGATCAAGGGCG‑GCGACAAGATGcagcagcag                                                                                                                        >  7:10445/1‑148 (MQ=255)                                tGCTCGAGTCGGATGCCGCCAAGAAGTATGCGGTTGA‑TGCCGAGAAGAAATTCGGTCCGCAACTGACAAAGC‑TGAAAAGCCTGGAAAGCAGTGCCAAGGGCATCCAGGACCGCCTGATCAAGGGCG‑GCGACAAGAAGCAGCAGCAGGAg                                                                                                                     >  3:44816/1‑149 (MQ=255)                                     gAGTCGGATGCCGCCAAGAAGTATGCGGTTGA‑TGCCGAGAAGAAATTCGGTCCGCAACTGACCAAGC‑TGAAAAGCCTGGAAAGCAGTGCCAAGGGCATCCAGGACCGCCTGATCAAGGGCG‑GCGACAAGATGCAGCAGCAGGAGCGTg                                                                                                                 <  7:166721/148‑1 (MQ=255)                                           gATGCCGCCAAGAAGTATGCGGTTGA‑TGCCGAGAAGAAATTCGGTCCGCAACTGACCAAGC‑TGAAAAGCCTGGAAAGCAGTGCCAAGGGCATCCAGGACCGCCTGATCAAGGGCG‑GCGACAAGATGCAGCAGCAGGAGCGTGAGCGCCt                                                                                                          >  2:73296/1‑149 (MQ=255)                                           gATGCCGCCAAGAAGTATGCGGTTGA‑TGCCGAGAAGAAATTCGGTCCGCAACTGACCAAGC‑TGAAAAGCCTGGAAAGCAGTGCCAAGGGCATCCAGGACCGCCTGATCAAGGGCG‑GCGACAAGATGCAGCAGCAGGAGCGTGAGCGCCt                                                                                                          <  1:1769/149‑1 (MQ=255)                                              gccgccAAGAAGTATGCGGTTGA‑TGCCGAGAAGAAATTCGGTCCGCAACTGACCAAGC‑TGAAAAGCCTGGAAAGCAGTGCCAAGGGCATCCAGGACCGCCTGATCAAGGGCG‑GCGACAAGATGCAGCAGCAGGAGCGTGAGCGCCTg                                                                                                         >  7:132364/1‑147 (MQ=255)                                               ccgccAAGAAGTATGCGGTTGA‑TGCCGAGAAGAAATTCGGTCCGCAACTGACCAAGC‑TGAAAAGCCTGGAAAGCAGTGCCAAGGGCATCCAGGACCGCCTGATCAAGGGCG‑GCGACAAGATGCAGCAGCAGGAGCGTGAGCGCCTGGAg                                                                                                      <  2:310129/149‑1 (MQ=255)                                                  ccAAGAAGTATGCGGTTGA‑TGCCGAGAAGAAATTCGGTCCGCAACTGACCAAGC‑TGAAAAGCCTGGAAAGCAGTGCCAAGGGCATCCAGGACCGCCTGATCAAGGGCG‑GCGACAAGATGCAGCAGCAGGAGCGTGAGCGCCTGGAGCTc                                                                                                   <  3:7814/149‑1 (MQ=255)                                                    aagaagTATGCGGTTGA‑TGCCGAGAAGAAATTCGGTCCGCAACTGACCAAGC‑TGAAAAGCCTGGAAAGCAGTGCCAAGGGCATCCAGGACCGCCTGATCAAGGGCG‑GCGACAAGATGCAGCAGCAGGAGCGTGAGCGCCTGGAGCTCGa                                                                                                 <  8:132364/149‑1 (MQ=255)                                                     agaagTATGCGGTTGA‑TGCCGAGAAGAAATTCGGTCCGCAACTGACCAAGC‑TGAAAAGCCTGGAAAGCAGTGCCAAGGGCATCCAGGACCGCCTGATCAAGGGCGCGCGACAAGATGCAGCAGCAGGAGCGTGAGCGCCTGGAGCTCGa                                                                                                 <  1:73296/149‑1 (MQ=255)                                                     agaagTATGCGGTTGA‑TGCCGAGAAGAAATTCGGTCCGCAACTGACCAAGC‑TGAAAAGCCTGGAAAGCAGTGCCAAGGGCATCCAGGACCGCCTGATCAAGGGCG‑GCGACAAGATGCAGCAGCAGGAGCGTGAGCGCCTGGAGCTCGAg                                                                                                <  7:58854/149‑1 (MQ=255)                                                         gTATGCGGTTGA‑TGCCGAGAAGAAATTCGGTCCGCAACTGACCAAGC‑TGAAAAGCCTGGAAAGCAGTGCCAAGGGCATCCAGGACCGCCTGATCAAGGGCG‑GCGACAAGATGCAGCAGCAGGAGCGTGAGCGCCTgg                                                                                                        >  5:372379/1‑137 (MQ=255)                                                         gTATGCGGTTGA‑TGCCGAGAAGAAATTCGGTCCGCAACTGACCAAGC‑TGAAAAGCCTGGAAAGCAGTGCCAAGGGCATCCAGGACCGCCTGATCAAGGGCG‑GCGACAAGATGCAGCAGCAGGAGCGTGAGCGCCTgg                                                                                                        <  6:372379/137‑1 (MQ=255)                                                         gTATGCGGTTGA‑TGCCGAGAAGAAATTCGGTCCGCAACTGACCAAGC‑TGAAAAGCCTGGAAAGCAGTGCCAAGGGCATCCAGGACCGCCTGATCAAGGGCG‑GCGACAAGATGCAGCAGCAGGAGCGTGAGCGCCTGGAGCTCGAGTTCa                                                                                            <  7:73076/149‑1 (MQ=255)                                                           aTGGGGTTGA‑TGCCGAGAAGAAATTCGGTCCGCAACTGACCAAGC‑TGAAAAGCCTGGAAAGCAGTGCCAAGGGCATCCAGGACCGCCTGATCAAGGGCG‑GCGACAAGATGCAGCAGCAGGAGCGTGAGCGCCTGGAGCTCGAGTTCaa                                                                                           <  6:369030/148‑1 (MQ=255)                                                           aTGCGGTTGA‑TGCCGAGAAGAAATTCGGTCCGCAACTGACCAAGC‑TGAAAAGCCTGGAAAGCAGTGCCAAGGGCATCCAGGACCGCCTGATCAAGGGCG‑GCGACAAGATGCAGCAGCAGGAGCGTGAGCGCCTGGAGCTCGAGTTCAAg                                                                                          >  8:74256/1‑149 (MQ=255)                                                            tGCGGTTGA‑TGCCGAGAAGAAATTCGGTCCGCAACTGACCAAGC‑TGAAAAGCCTGGAAAGCAGTGCCAAGGGCATCCAGGACCGCCTGATCAAGGGCG‑GCGACAAGATGCAGCAGCAGGAGCGTGAGCGCCTGGAGCTCGAGTTCAAGc                                                                                         <  2:114403/149‑1 (MQ=255)                                                             gCGGTTGA‑TGCCGAGAAGAAATTCGGTCCGCAACTGACCAAGC‑TGAAAAGCCTGGAAAGCAGTGCCAAGGGCATCCAGGACCGCCTGATCAAGGGCG‑GCGACAAGATGCAGCAGCAGGAGCGTGAGCGCCTGGAGCTCGAGTTCAAGc                                                                                         >  8:333844/1‑148 (MQ=255)                                                                 ttGA‑TGCCGAGAAGAAATTCGGTCCGCAACTGACCAAGC‑TGAAAAGCCTGGAAAGCAGTGCCAAGGGCATCCAGGACCGCCTGATCAAGGGCG‑GCGACAAGATGCAGCAGCAGGAGCGTGAGCGCCTGGAGCTCGAGTTCAAGCAAAAg                                                                                    <  5:76770/149‑1 (MQ=255)                                                                       gtcGAGAAGAAATTCGGTCCGCAACTGACCAAGC‑TGAAAAGCCTGGAAAGCAGTGCCAAGGGCATCCAGGACCGCCTGATCAAGGGCG‑GCGACAAGATGCAGCAGCAGGAGCGTGAGCGCCTGGAGCTCGAGTTCAAGCAAAAGGCCcg                                                                               >  7:301260/3‑149 (MQ=255)                                                                          gagaAGAAATTCGGTCCGCAACTGACCAAGC‑TGAAAAGCCTGGAAAGCAGTGCCAAGGGCATCCAGGACCGCCTGGTCAAGGGCG‑GCGACAAGATGCAGCAGCAGGAGCGTGAGCGCCTGGAGCTCGAGTTCCAGCAAAAGGCCCGCGa                                                                            >  6:353901/1‑149 (MQ=255)                                                                          gagaAGAAATTCGGTCCGCAACTGACCAAGC‑TGAAAAGCCTGGAAAGCAGTGCCAAGGGCATCCAGGACCGCCTGGTCAAGGGCG‑GCGACAAGATGCAGCAGCAGGAGCGTGAGCGCCTGGAGCTCGAGTTCAAGCAAAAGGCCCGCGa                                                                            >  6:353898/1‑149 (MQ=255)                                                                          gagaAGAAATTCGGTCCGCAACTGACCAAGC‑TGAAAAGCCTGGAAAGCAGTGCCAAGGGCATCCAGGACCGCCTGATCAAGGGCG‑GCGACAAGATGCAGCAGCAGGAGCGTGAGCGCCTGGAGCTCGAGTTCAAGCAAAAGGCCCGCGa                                                                            >  6:117899/1‑149 (MQ=255)                                                                               gaaATTCGGTCCGCAACTGACCAAGCGTGAAAAGCCTGGAAAGCAGTGCCAAGGGCATCCAGGACCGCCTGATCAAGGGCG‑GCGACAAGATGCAGCAGCAGGAGCGTGAGCGCCTGGAGCTCGAGTTCAAGCAAAAGGCCCGCGATttc                                                                        >  1:5158/1‑149 (MQ=255)                                                                                aaaTTCGGTCCGCAACTGACCAAGC‑TGAAAAGCCTGGAAAGCAGTGCCAAGGGCATCCAGGACCGCCTGATCAAGGGCG‑GCGACAAGATGCAGCAGCAGGAGCGTGAGCGCCTGGAGCTCGAGTTCAAGCAAAAGGCCCGCGATttc                                                                        >  7:452/1‑147 (MQ=255)                                                                                aaaTTCGGTCCGCAACTGACCAAGC‑TGAAAAGCCTGGAAAGCAGTGCCAAGGGCATCCAGGACCGCCTGATCAAGGGCG‑GCGACAAGATGCAGCAGCAGGAGCGTGAGCGCCTGGAGCTCGAGTTCAAGCAAAAGGCCCGCGATttc                                                                        >  7:5734/1‑147 (MQ=255)                                                                                 aaTTCGGTCCGCAACTGACCAAGC‑TGAAAAGCCTGGAAAGCAGTGCCAAGGGCATCCAGGACCGCCTGATCAAGGGCG‑GCGACAAGATGCAGCAGCAGGAGCGTGAGCGCCTGGAGCTCGAGTTCAAGCAAAAGGCCCGCGATttccag                                                                     <  2:5158/149‑1 (MQ=255)                                                                                  aTTCGGTCCGCAACTGACCAAGC‑TGAAAAGCCTGGAAAGCAGTGCCAAGGGCATCCAGGACCGCCTGATCAAGGGCG‑GCGACAAGATGCAGCAGCAGGAGCGTGAGCGCCTGGAGATCGAGTTCAAGCAAAAGGCCCGCGATttccagt                                                                    >  6:283517/1‑149 (MQ=255)                                                                                      ggTCCGCAACTGACCAAGC‑TGAAAAGCCTGGAAAGCAGTGCCAAGGGCATCCAGGACCGCCTGATCAAGGGCG‑GCGACAAGATGCAGCAGCAGGAGCGTGAGCGCCTGGAGCTCGAGTTCAAGCAAAAGGCCCGCGATttccagttc                                                                  >  7:305506/1‑147 (MQ=255)                                                                                           gCAACTGACCAAGC‑TGAAAAGCCTGGAAAGCAGTGCCAAGGGCATCCAGGACCGCCTGATCAAGGGCG‑GCGACAAGATGCAGCAGCAGGAGCGTGAGCGCCTGGAGCTCGAGTTCAAGCAAAAGGCCCGCGATTTCCAGTTCCAGTcc                                                            >  7:213537/1‑148 (MQ=255)                                                                                            cAACTGACCAAGC‑TGAAAAGCCTGGAAAGCAGTGCCAAGGGCATCCAGGACCGCCTGATCAAGGGCG‑GCGACAAGATGCAGCAGCAGGAGCGTGAGCGCCTGGAGCTCGAGTTCAAGCAAAAGGCCCGCGATTTCCAGTTCCAGTCCaa                                                          <  2:304486/149‑1 (MQ=255)                                                                                             aaCTGACCAAGC‑TGAAAAGCCTGGAAAGCAGTGCCAAGGGCATCCAGGACCGCCTGATCAAGGGCG‑GCGACAAGATGCAGCAGCAGGAGCGTGAGCGCCTGGAGCTCGAGTTCAAGCAAAAGGCCCGCGATTTCCAGTTCCAGTCCAAg                                                         >  7:213666/1‑149 (MQ=255)                                                                                               cTGACCAAGC‑TGAAAAGCCTGGAAAGCAGTGCCAAGGGCATCCAGGACCGCCTGATCAAGGGCG‑GCGACAAGATGCAGCAGCAGGAGCGTGAGCGCCTGGAGCTCGAGTTCAAGCAAAAGGCCCGCGATTTCCAGTTCCAGTCCAAGGa                                                       <  8:452/149‑1 (MQ=255)                                                                                               cTGACCAAGC‑TGAAAAGCCTGGAAAGCAGTGCCAAGGGCATCCAGGACCGCCTGATCAAGGGCG‑GCGACAAGATGCAGCAGCAGGAGCGTGAGCGCCTGGAGCTCGAGTTCAAGCAAAAGGCCCGCGATTTCCAGTTCCAGTCCAAGGa                                                       <  8:5734/149‑1 (MQ=255)                                                                                                  aCCAAGC‑TGAAAAGCCTGGAAAGCAGTGCCAAGGGCATCCAGGACCGCCTGATCAAGGGCG‑GCGACAAGATGCAGCAGCAGGAGCGTGAGCGCCTGGAGCTCGAGTTCAAGCAAAAGGCCCGCGATTTCCAGTTCCAGTCCAAGGaa                                                      <  3:342566/147‑1 (MQ=255)                                                                                                   ccAAGC‑TGAAAAGCCTGGAAAGCAGTGCCAAGGGCATCCAGGACCGCCTGATCAAGGGCG‑GCGACAAGATGCAGCAGCAGGAGCGTGAGCGCCTGGAGCTCGAGTTCAAGCAAAAGGCCCGCGATTTCCAGTTCCAGTCCAAGGAACt                                                    >  6:33145/1‑148 (MQ=255)                                                                                                    cAAGC‑TGAAAAGCCTGGAAAGCAGTGCCAAGGGCATCCAGGACCGCCTGATCAAGGGCG‑GCGACAAGATGCAGCAGCAGGAGCGTGAGCGCCTGGAGCTCGAGTTc                                                                                             <  5:37886/106‑1 (MQ=255)                                                                                                    cAAGC‑TGAAAAGCCTGGAAAGCAGTGCCAAGGGCATCCAGGACCGCCTGATCAAGGGCG‑GCGACAAGATGCAGCAGCAGGAGCGTGAGCGCCTGGAGCTCGAGTTc                                                                                             >  6:37886/1‑106 (MQ=255)                                                                                                    cAAGC‑TGAAAAGCCTGGAAAGCAGTGCCAAGGGCATCCAGGACCGCCTGATCAAGGGCG‑GCGACAAGATGCAGCAGCAGGAGCGTGAGCGCCTGGAGCTCGAGTTCAAGCAAAAGGCCCGCGATTTCCAGTTCCAGTCCAAGGAACTg                                                   >  3:257784/1‑148 (MQ=255)                                                                                                    cAAGC‑TGAAAAGCCTGGAAAGCAGTGCCAAGGGCATCCAGGACCGCCTGATCAAGGGCG‑GCGACAAGATGCAGCAGCAGGAGCGTGAGCGCCTGGAGCTCGAGTTCAAGCAAAAGGCCCGCGATTTCCAGTTCCAGTCCAAGGAACTg                                                   >  4:207078/1‑148 (MQ=255)                                                                                                     aaGC‑TGAAAAGCCTGGAAAGCAGTGCCAAGGGCATCCAGGACCGCCTGATCAAGGGCG‑GCTACAAGATGCAGCAGCAGGAGCGTGAGCGCCTGGAGCTCGAGTTCAAGCAAAAGGCCCGCGATTTCCAGTTCCAGTCCAAGGAACTGaa                                                 <  8:301260/149‑1 (MQ=255)                                                                                                     aaGC‑TGAAAAGCCTGGAAAGCAGTGCCAAGGGCATCCAGGACCGCCTGATCAAGGGCG‑GCGACAAGATGCAGCAGCAGGAGCGTGAGCGCCTGGAGCTCGAGTTCAAGCAAAAGGCCCGCGATTTCCAGTTCCAGTCCAAGGAACTGaa                                                 >  7:241501/1‑149 (MQ=255)                                                                                                      aGC‑TGAAAACCATGGAAAGCAGTGCCAAGGGCATCCAGGACCGACTGATCAAGGGCG‑GCGACAATATGCAGCAGCAGGAGCGTGAGCTCCTGGAGCTCGAGTTCAAGCAAAAGGCCCGCGATTTCCAGTTCCAGTCCAAGGAACTGAAc                                                <  4:44816/149‑1 (MQ=255)                                                                                                          tGAAAAGCCTGGAAAGCAGTGCCAAGGGCATCCAGGACCGCCTGATCAAGGGCG‑GCGACAAGATGCAGCAGCAGGAGCGTGAGCGCCTGGAGCTCGAGTTCa                                                                                            >  3:268712/1‑102 (MQ=255)                                                                                                          tGAAAAGCCTGGAAAGCAGTGCCAAGGGCATCCAGGACCGCCTGATCAAGGGCG‑GCGACAAGATGCAGCAGCAGGAGCGTGAGCGCCTGGAGCTCGAGTTCa                                                                                            <  4:268712/102‑1 (MQ=255)                                                                                                                gCCTGGAAAGCAGTGCCAAGGGCATCCAGGACCGCCTGATCAAGGGCG‑GCGACAAGATGCAGCAGCAGGAGCGTGAGTGCCTGGAGCTCGAGTTCAAGCAAAAGGCCCGCGATTTCAAGTTCCAGTACAAGGAACTGAACGAATCCatc                                       >  4:247464/1‑147 (MQ=255)                                                                                                                 ccTGGAAAGCAGTGCCAAGGGCATCCAGGACCGCCTGATCAAGGGCG‑GCGACAAGATGCAGCAGCAGGAGCGTGAGCGCCTGGAGCTCGAGTTCAAGCAAAAGGCCCGCGATttcc                                                                       <  2:300388/116‑1 (MQ=255)                                                                                                                 ccTGGAAAGCAGTGCCAAGGGCATCCAGGACCGCCTGATCAAGGGCG‑GCGACAAGATGCAGCAGCAGGAGCGTGAGCGCCTGGAGCTCGAGTTCAAGCAAAAGGCCCGCGATttcc                                                                       >  1:300388/1‑116 (MQ=255)                                                                                                                     gAAAGCAGTGCCAAGGGCATCCAGGACCGCCTGATCAAGGGCG‑GCGACAAGATGCAGCAGCAGGAGCGTGAGCGCCTGGAGCTCGAGTTCAAGCAAAAGGCCCGCGATTTCCAGTTCCAGTCCAAGGAACTGAACGAAGCCAAGGCCGt                                  >  8:235711/1‑149 (MQ=255)                                                                                                                       aaGCAGTGCCAAGGGCATCCAGGACCGCCTGATCAAGGGCG‑GCGACAAGATGCAGCAGCAGGAGCGTGAGCGCCTGGAGCTCGAGTTCAAGCAAAAGGCCCGCGATTTCCAGTTCCAGTCCAAGGAACTGAACGAAGCCAAGGCCGTTg                                <  7:333844/149‑1 (MQ=255)                                                                                                                           aGTGCCAAGGGCATCCAGGACCGCCTGATCAAGGGCG‑GCGACAAGATGCAGCAGCAGGAGCGTGAGCGCCTGGAGCTCGAGTTCAAGCAAAAGGCCCGCGATTTCCAGTTCCAGTCCAAGGAACTGAACGAAGCCAAGGCCGTTGCTGa                            >  3:10006/1‑149 (MQ=255)                                                                                                                           aGTGCCAAGGGCATCCAGGACCGCCTGATCAAGGGCG‑GCGACAAGATGCAGCAGCAGGAGCGTGAGCGCCTGGAGCTCGAGTTCAAGCAAAAGGCCCGCGATTTCCAGTTCCAGTCCAAGGAACTGAACGAAGCCAAGGCCGTTGCTGa                            <  1:260852/149‑1 (MQ=255)                                                                                                                             tGCCAAGGGCATCCAGGACCGCCTGATCAAGCGCG‑GCGACAAGATGCAGCAGCAGGAGCGTCAGCGCCTGGAGCACGAGTTCAAGCAAAAGCCCCGCGATTTCCAGTTCCAGTCCAAGGAACTGAACGAAGACAAGGCCGTTGCTGATc                          >  8:184555/1‑149 (MQ=255)                                                                                                                               ccAAGGGCATCCAGGACCGCCTTGTCAAGGGCG‑GCTACTATTTGCAGAAGCAGTAGCGTTAGCGCCTGTAGCTCGAGTTCAAGCAAAAGGCCCGCTATTTCCAGTTCCAGTCCAAGGAACTGAACGAAGCCAAGGCCGTTGCTGATctc                        <  5:353901/149‑3 (MQ=255)                                                                                                                               ccAAGGGCATCCAGGACCGCCTGGTCAAGGGCG‑GCGACAAGATGCAGCAGCAGGAGCGTGAGCGCCTGGAGCTCGAGTTCAAGCAAAAGGCCCGCGATTTCCAGTTCCAGTCCAAGGAACTGAACGAAGCCAAGGCCGTTGCTGATcgc                        <  5:353898/149‑1 (MQ=255)                                                                                                                               ccAAGGGCATCCAGGACCGCCTGATCAAGGGCG‑GCGACAAGATGCAGCAGCAGGAGCGTGAGCGCCTGGAGCTCGAGTTCAAGCAAAAGGCCCGCGATTTCCAGTTCCAGTCCAAGGAACTGAACGAAGCCAAGGCCGTTGCTGATcgc                        >  5:278460/1‑149 (MQ=255)                                                                                                                                cAAGGGCATCCAGGACCGCCTGATCAAGGGCG‑GCGACAAGATGCAGCAGCAGGAGCGTGAGCGCCTGGAGCTCGAGTTCAAGCAAAAGGCCCGCGATTTCCAGTTCCAGTCCAAGGAACTGAACGAAGCCAAGGCCGTTGCTGATcgcg                       <  3:146491/149‑1 (MQ=255)                                                                                                                                 aaGGGCATCCAGGACCGCCTGATCAAGGGCG‑GCGACAAGATGCAGCAGCAGGAGCGTGAGCGCCTGGAGCTCGAGTTCAAGCAAAAGGCCCGCGATTTCCAGTTCCAGTCCAAGGAAc                                                     <  3:240921/118‑1 (MQ=255)                                                                                                                                 aaGGGCATCCAGGACCGCCTGATCAAGGGCG‑GCGACAAGATGCAGCAGCAGGAGCGTGAGCGCCTGGAGCTCGAGTTCAAGCAAAAGGCCCGCGATTTCCAGTTCCAGTCCAAGGAAc                                                     >  4:240921/1‑118 (MQ=255)                                                                                                                                    ggCATCCAGGACCGCCTGATCAAGGGCG‑GCGACAAGATGCAGCAGCAGGAGCGTGAGCGCCTGGAGCTCGAGTTCAAGCAAAAGGCCCGCGATTTCCAGTTCCAGTCCAAGGAACTGAACGAAGCCAAGGCCGTTGCTGATCGCGACAt                   >  4:40552/1‑149 (MQ=255)                                                                                                                                           aGGACCGCCTGATCAAGGGCG‑GCGACAAGATGCAGCAGCAGGAGCGTGAGCGCCTGGAGCTCGAGTTCAAGCAAAAGGCCCGCGATTTCCAGTTCCAGTCCAAGGAACTGAACGAAGCCAAGGCCGTTGCTGATCGCGACATGCTCAAg            >  2:238199/1‑149 (MQ=255)                                                                                                                                                cGCCTGATCAAGGGCG‑GCGACAAGATGCAGCAGCAGGAGCGTGAGCGCCTGGAGCTCGAGTTCAAGCAAAAGGCCCGCGATTTCCAGTTCCAGTCCAAGGAACTGAACGAAGCCAAGGCCGTTGCTGATCGCGACATGCTCAAGCAACt       <  8:10445/149‑1 (MQ=255)                                                                                                                                                 gCCTGATCAAGGGCG‑GCGACAAGATGCAGCAGCAGGAGCGTGAGCGCCTGGAGCTCGAGTTCAAGCAAAAGGCCCGCGATTTCCAGTTCCAGTCCAAGGAACTGAACGAAGCCAAGGCCGTTGATGATCGCGACATGCGAAAGCAACt       >  7:146671/1‑148 (MQ=255)                                                                                                                                                  ccTGATCAAGGGCG‑GCGACAAGATGCAGCAGCAGGAGCGTGAGCGCCTGGAGCTCGAGTTCAAGCAAAAGGCCCGCGATTTCCAGTTCCAGTCCAAGGAACTGAACGAAGCCAAGGCCGTTGCt                              <  8:299020/124‑1 (MQ=255)                                                                                                                                                  ccTGATCAAGGGCG‑GCGACAAGATGCAGCAGCAGGAGCGTGAGCGCCTGGAGCTCGAGTTCAAGCAAAAGGCCCGCGATTTCCAGTTCCAGTCCAAGGAACTGAACGAAGCCAAGGCCGTTGCTGATCGCGACATGCTCAAGCAACTg      >  8:206369/1‑148 (MQ=255)                                                                                                                                                  ccTGATCAAGGGCG‑GCGACAAGATGCAGCAGCAGGAGCGTGAGCGCCTGGAGCTCGAGTTCAAGCAAAAGGCCCGCGATTTCCAGTTCCAGTCCAAGGAACTGAACGAAGCCAAGGCCGTTGCTGATCGCGACATGCTCAAGCAACTg      >  1:341731/1‑148 (MQ=255)                                                                                                                                                   cTGATCAAGGGCG‑GCGACAAGATGCAGCAGCAGGAGCGTGAGCGCCTGGAGCTCGAGTTCAAGCAAAAGGCCCGCGATTTCCAGTTCCAGTCCAAGGAACTGAACGAAGCCAAGGCCGTTGCTGATCGCGACATGCTCAAGCAACTGaa    >  4:138732/1‑149 (MQ=255)                                                                                                                                                      aTCAAGGGCG‑GCGACAAGATGCAGCAGCAGGAGCGTGAGCGCCTGGAGCTCGAGTTCAAGCAAAAGGCCCGCGATTTCCAGTTCCAGTCCAAGGAACTGAACGAAGCCAAGGCCGTTGCTGATCGCGACATGCTCAAGCAACTGAAg   <  8:241501/147‑1 (MQ=255)                                                                                                                                                      aTCAAGGGCG‑GCGACAAGATGCAGCAGCAGGAGCGTGAGCGCCTGGAGCTCGAGTTCAAGCAAAAGGCCCGCGATTTCCAGTTCCAGTCCAAGGAACCGAACGAAGCCAAGGCCGTTGCTGATCGCGACATGCTCAAGCAACTGAAGc  <  8:213666/148‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                     AAGGTTGCCGTCCTGAACTACCAGATGGCCCTGCTCGAGTCGGATGCCGCCAAGAAGTATGCGGTTGA‑TGCCGAGAAGAAATTCGGTCCGCAACTGACCAAGC‑TGAAAAGCCTGGAAAGCAGTGCCAAGGGCATCCAGGACCGCCTGATCAAGGGCG‑GCGACAAGATGCAGCAGCAGGAGCGTGAGCGCCTGGAGCTCGAGTTCAAGCAAAAGGCCCGCGATTTCCAGTTCCAGTCCAAGGAACTGAACGAAGCCAAGGCCGTTGCTGATCGCGACATGCTCAAGCAACTGAAGC  >  NC_002947/1795257‑1795551

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑