Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,835,637 A→G 7.8% intergenic (‑79/+53) PP_1639 ← / ← yohC SprT family protein/inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,835,6370AG 7.8% 173.3 / 3.5 64intergenic (‑79/+53)PP_1639/yohCSprT family protein/inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (30/29);  new base G (2/3);  total (32/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCGTTTGAAAAAGGCTTCGGCTTGCTGGTAACAGGTTTCGACGCGTTGTTTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGATTGCCACCAGTACTACCAAGCCCACTGCCAGCACC  >  NC_002947/1835492‑1835783
                                                                                                                                                 |                                                                                                                                                  
ggcgTTTGAAAAAGGCTTCGGCTTGCTGGTAACAGGTTTCGACGCGTTGTTTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGgc                                                                                                                                                 >  6:330425/1‑149 (MQ=255)
 gcgTTTGAAAAAGGCTTCGGCTTGCTGGTAACAGGTTTCGACGCGTTGTGTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATTCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGgcc                                                                                                                                                <  3:345965/149‑1 (MQ=255)
   gTTTGAAAAAGGCTTCGGCTTGCTGGTAACAGGTTTCGACGCGTTGTTTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGgccgc                                                                                                                                              >  8:346099/1‑149 (MQ=255)
       gAAATAGGCTTCGGCTTGCTGGTAACAGGTTTCGACGCGTTGTTTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGGCAGCGCACAGCCAGAGGCCGCCtt                                                                                                                                           <  4:117852/148‑1 (MQ=255)
       gAAAAAGGCTTCGGCTTGCTGGTAACAGGTTTCGACGCGTTGTTTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCtt                                                                                                                                           >  1:242151/1‑148 (MQ=255)
       gAAAAAGGCTTCGGCTTGCTGGTAACAGGTTTCGACGCGTTGTTTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCtt                                                                                                                                           <  2:242151/148‑1 (MQ=255)
         aaaaGGCTTCGGCTTGCTGGTAACAGGTTTCGACGCGTTGTTTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTgcg                                                                                                                                        >  2:57804/1‑149 (MQ=255)
         aaaaGGCTTCGGCTTGCTGGTAACAGGTTTCGACGCGTTGTTTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTgcg                                                                                                                                        >  4:293599/1‑149 (MQ=255)
                     cTTGCTGGTAACAGGTTTCGACGCGTTGTGTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGt                                                                                                                            <  3:224578/149‑1 (MQ=255)
                      ttGCTGGTAACAGGTTTCGACGCGTTGTTTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGtt                                                                                                                           >  1:53311/1‑149 (MQ=255)
                        gCTGGTAACAGGTTTCGACGCGTTGTTTGAGCTGCTCGGGCATGGCGGGGCGGGTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTg                                                                                                                          <  7:185594/148‑1 (MQ=255)
                         cTGGTAACAGGTTTCGACGCGTTGTTTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGGGGCCGCCGCAAGGCGGCCTCTGGCTgt                                                                                                                         <  7:200463/148‑2 (MQ=255)
                          tGGTAACAGGTTTCGACGCGTTGTTTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATg                                                                                                                       >  1:243169/1‑149 (MQ=255)
                             tAACCGGTTTCGACGCGTTGTTTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGccc                                                                                                                    <  6:216495/149‑1 (MQ=255)
                               aCAGGTTTCGACGCGTTGTTTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCa                                                                                                                  <  5:60919/149‑1 (MQ=255)
                               aCAGGGTTCGACGCGTTGTTTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCa                                                                                                                  <  7:297104/149‑1 (MQ=255)
                                      tCGACGCGTTGTTTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTg                                                                                                            <  2:184530/148‑1 (MQ=255)
                                      tCGACGCGTTGTTTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTg                                                                                                            <  5:330425/148‑1 (MQ=255)
                                                gttTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTg                                                                                                  <  7:346099/148‑1 (MQ=255)
                                                   tGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTGCGGTCTGTTc                                                                                               >  6:177682/1‑148 (MQ=255)
                                                     aGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAg                                                                                            <  5:210168/149‑1 (MQ=255)
                                                      gctgctCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGt                                                                                           >  5:244246/1‑149 (MQ=255)
                                                      gctgctCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGt                                                                                           >  5:107225/1‑149 (MQ=255)
                                                      gctgctCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGt                                                                                           <  6:244246/149‑1 (MQ=255)
                                                      gctgctCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGt                                                                                           >  8:107331/1‑149 (MQ=255)
                                                          ctCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGgt                                                                                       >  5:44695/1‑149 (MQ=255)
                                                               gCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGGGGCCGCCGCAAGGCGGCCTCTgg                                                                                                                             >  2:359171/1‑106 (MQ=38)
                                                               gCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGGGGCCGCCGCAAGGCGGCCTCTgg                                                                                                                             <  1:359171/106‑1 (MQ=38)
                                                               gCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGa                                                                                  <  5:24616/149‑1 (MQ=255)
                                                                 aTGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGgt                                                                                       <  4:237529/142‑1 (MQ=255)
                                                                 aTGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGgt                                                                                       >  3:237529/1‑142 (MQ=255)
                                                                   ggcggggcggTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACggg                                                                              >  5:306999/1‑149 (MQ=255)
                                                                    gcggggcggTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGc                                                                             <  3:293599/149‑1 (MQ=255)
                                                                      ggggcggTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGccc                                                                           >  6:57154/1‑149 (MQ=255)
                                                                               ctcCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGcc                                                                     >  8:260840/1‑146 (MQ=255)
                                                                               ctcCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCa                                                                   <  2:53311/148‑1 (MQ=255)
                                                                               ctcCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCa                                                                   <  6:107225/148‑1 (MQ=255)
                                                                                 ccTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGACGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTc                                                                >  8:160464/1‑149 (MQ=255)
                                                                                  cTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTcc                                                               >  3:133830/1‑149 (MQ=255)
                                                                                  cTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTcc                                                               >  8:321281/1‑149 (MQ=255)
                                                                                        aGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTCCCCAGa                                                          >  4:296020/1‑148 (MQ=255)
                                                                                        aGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTCCCCAGa                                                          >  1:239922/1‑148 (MQ=255)
                                                                                            aTTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTc                                                                <  2:154426/138‑1 (MQ=255)
                                                                                            aTTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTc                                                                >  1:154426/1‑138 (MQ=255)
                                                                                             ttATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTCCCCAGATAATCa                                                    >  4:199003/1‑149 (MQ=255)
                                                                                               aTGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTCCCCAGATAATCAc                                                   <  1:57804/148‑1 (MQ=255)
                                                                                                tGCCGCAAGCGCAGGTGGCTTGCCAAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTCCCCAGATAATCACgg                                                 >  7:231107/1‑149 (MQ=255)
                                                                                                  ccGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGTGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTCCCCAGATAATCACgg                                                 >  5:280954/1‑147 (MQ=255)
                                                                                                   cGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAg                                              >  2:344873/1‑149 (MQ=255)
                                                                                                   cGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAg                                              <  5:57154/149‑1 (MQ=255)
                                                                                                   cGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAg                                              >  4:331733/1‑149 (MQ=255)
                                                                                                              gTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGAtt                                   <  7:107331/149‑1 (MQ=255)
                                                                                                                ggCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGATTGc                                 <  8:188914/149‑1 (MQ=255)
                                                                                                                ggCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGATTGc                                 <  7:260840/149‑1 (MQ=255)
                                                                                                                           gcACCGGTCAGCGCACAGCCAGATGCCGCCTTGGGGCGGCCCCTGGGTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGATTGCCACCAGtacta                      <  5:177682/148‑1 (MQ=255)
                                                                                                                              ccGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGATTGCCACCAGTACTAcc                    >  6:137856/1‑148 (MQ=255)
                                                                                                                               cGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGATTGCCACCAGTACTACCaa                  <  7:160464/149‑1 (MQ=255)
                                                                                                                                 gTCAGCGCACAGCCAGAGGCGGCATTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGATTGCCACCAGTACTACCAAGc                <  5:305794/149‑1 (MQ=255)
                                                                                                                                  tCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGATTGCCACCAGTACTACCAAGcc               <  2:239922/149‑1 (MQ=255)
                                                                                                                                  tCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGATTGCCACCAGTACTACCAAGcc               <  3:331733/149‑1 (MQ=255)
                                                                                                                                      cgcACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGATTGCCACCAGTACTACCAAGCCCACt           >  6:217905/1‑149 (MQ=255)
                                                                                                                                        cacaGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGATTGCCACCAGTACTACCAAGCCCACTGc         >  1:231986/1‑149 (MQ=255)
                                                                                                                                         tcaaccAGGGGCCGCCGCAAGGCGGCCTCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGATTGCCACCAGTACTACCAAGCCCACTGc         <  7:109242/144‑1 (MQ=255)
                                                                                                                                         tcaaccAGGGGCCGCCGCAAGGCGGCCTCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGATTGCCACCAGTACTACCAAGCCCACTGc         >  8:109242/5‑148 (MQ=255)
                                                                                                                                                gagGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGATTGCCACCAGTACTACCAAGCCCACTGCCAGCAcc  >  6:295723/1‑148 (MQ=255)
                                                                                                                                                gagGCCGCCTTGCGGCGGCCCCTGGTAGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGATTGCCACCAGTACTACCAAGCCCACTGCCCGCAcc  >  3:310430/1‑148 (MQ=255)
                                                                                                                                                 agGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGATTGCCACCAGTACTACCAAGCCCACTGCCAGCAcc  >  6:64357/1‑147 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
GGCGTTTGAAAAAGGCTTCGGCTTGCTGGTAACAGGTTTCGACGCGTTGTTTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGATTGCCACCAGTACTACCAAGCCCACTGCCAGCACC  >  NC_002947/1835492‑1835783

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑