Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,921,549 T→A 12.9% intergenic (+24/+37) PP_1720 → / ← PP_1721 alcohol dehydrogenase/phosphoserine phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,921,5490TA12.9% 81.9 / 6.5 39intergenic (+24/+37)PP_1720/PP_1721alcohol dehydrogenase/phosphoserine phosphatase
Reads supporting (aligned to +/- strand):  ref base T (17/17);  new base A (2/3);  total (19/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.02e-01

GCGGTTGTCGCCGCAGCTGGTCGACACCTATCCGGTGGAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCG‑CGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCGGGTGATCCACTCTCAACAACAACGG  >  NC_002947/1921402‑1921687
                                                                                                                                                    |                                                                                                                                          
gCGGTTGTCGCCGCAGCTGGTCGACACCTATCCGGTGGAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCG‑CGGGttt                                                                                                                                           <  8:233878/149‑1 (MQ=255)
    ttGTCGCCGCAGCTGGTCGACACCTATCCGGTGGAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCG‑CGGGTTTAccc                                                                                                                                       <  8:236007/149‑1 (MQ=255)
       tCGCCGCAGCTGGTCGACACCTATCCGGTGGAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCG‑CGGGTTTACCcgcg                                                                                                                                    <  8:312447/149‑1 (MQ=255)
         gccgcAGCTGGTCGACACCTATCCGGTGGAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCG‑CGGGTTTACCcgcg                                                                                                                                    >  3:173464/1‑147 (MQ=255)
         gccgcAGCTGGTCGACACCTATCCGGTGGAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCG‑CGGGTTTACCcgcg                                                                                                                                    >  6:318966/1‑147 (MQ=255)
         gccgcAGCTGGTCGACACCTATCCGGTGGAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCG‑CGGGTAAACCCGCGaa                                                                                                                                  <  2:9407/149‑1 (MQ=255)
         gccgcAGCTGGTCGACACCTATCCGGTGGAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCCGGCCCCTTCG‑CGGGTAAACCCGCGaa                                                                                                                                  <  2:345307/149‑1 (MQ=255)
          ccgcAGCTGGTCGACACCTATCCGGTGGAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGGCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCG‑CGTGTAAACCCGCGAAg                                                                                                                                 <  5:184538/149‑1 (MQ=255)
                cTGGTCGACACCTATCCGGGGGAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCG‑CGGGTTTACCCGCGAAGGGGCCg                                                                                                                           <  7:48357/149‑1 (MQ=255)
                   gTCGACACCTATCCGGTGGAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCG‑CGGGTTTACCCGCGAAGGGGCCGGca                                                                                                                        >  5:258984/1‑149 (MQ=255)
                           cTATCCGGTGGAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGCGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCG‑CGGGTTTACCCGCGAAGGGGCCGGCACAGCCCa                                                                                                                 >  7:338123/1‑148 (MQ=255)
                            tATCCGGTGGAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCG‑CGGGTTTACCCGCGAAGGGGCCGGCACAGCCCAt                                                                                                                >  5:337856/1‑148 (MQ=255)
                                    ggAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCG‑CGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCtt                                                                                                       <  5:318966/149‑1 (MQ=255)
                                      aGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCG‑CGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTc                                                                                                      <  6:258984/148‑1 (MQ=255)
                                       gTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTTTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCG‑CGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTcag                                                                                                    >  5:366904/1‑149 (MQ=255)
                                       gTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTca                                                                                                     <  1:267719/149‑1 (MQ=255)
                                       gTTTGCCCAGGCGGCGTATGCCGAGCTGAAGAACAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCG‑CGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTcag                                                                                                    >  4:82134/1‑149 (MQ=255)
                                                 gcggcgTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTACGCGGGGCTGGCCCCTTCG‑CGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCa                                                                                          <  4:247175/149‑1 (MQ=255)
                                                      ttATGCCGCGCTGGTGACCACCCCGGTGTGGGGCATGCTGGTTATGGTGATCCACCCTCGCCTGGTGTAAGCGGGGCTGGCCCGTGCG‑CGGGTTTACCCGCGATGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTc                                                                                     <  4:173464/148‑1 (MQ=255)
                                                                cTGGAGACCAACCCGGGGTGGGGCAAGCTGGTGATGGTGACCGACCCTCGCCGGGTGAACGCGGGGCGGGCCCCTTCG‑GGGTTTAACCCGCGACGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCAt                                                                           <  3:367576/149‑1 (MQ=255)
                                                                  ggAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCG‑CGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCAtt                                                                          >  7:348508/1‑148 (MQ=255)
                                                                   gagaCCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGGGGGGCTGGCCCCTTCG‑CGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTg                                                                         <  8:2488/148‑1 (MQ=255)
                                                                     gaCCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCT‑CGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGAtc                                                                      <  6:366904/149‑1 (MQ=255)
                                                                        caaccaGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCG‑CGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCg                                                                   >  2:35872/1‑149 (MQ=255)
                                                                        caaccaGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCG‑CGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCg                                                                   >  4:77005/1‑149 (MQ=255)
                                                                         aaccaGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCG‑CGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCgg                                                                  >  3:181138/1‑149 (MQ=255)
                                                                              ggTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCG‑CGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGc                                                             <  6:337856/149‑1 (MQ=255)
                                                                                                    gtgatCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCG‑CGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGt                                       >  8:200480/1‑149 (MQ=255)
                                                                                                       ttCGACCCTTGCCTGGTGTAAGCGGTGCTGGCCCCTTCG‑CTGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGGCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGttga                                    <  7:170975/148‑3 (MQ=255)
                                                                                                              cTAGCCTGGTGTACGCGGGGCTGGCCCCTTCG‑CGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATg                             <  3:82134/149‑1 (MQ=255)
                                                                                                                 gCCTGGTGTAAGCGGGGCTGGCCCCTTCG‑CGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCgg                          <  7:200480/149‑1 (MQ=255)
                                                                                                                  ccTGGTGTAAGCGGGGCTGGCCCCTTCG‑CGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCgg                                         <  6:118326/133‑1 (MQ=255)
                                                                                                                  ccTGGTGTAAGCGGGGCTGGCCCCTTCG‑CGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCgg                                         >  5:118326/1‑133 (MQ=255)
                                                                                                                    tGGTGTAAGCGGGGCTGGCCCCTTCG‑CGGGTTTACCGGCGAAGGGGCCGCCACAGCCCAGAAAACCCTTCACCTCCCGGGCCGAGTCGGCCACCCATTGAAAGGGGAATGCTGGGGCAGCACTGGGTCCGGGTTAACCACTTCCggtgg                       >  6:75580/1‑146 (MQ=255)
                                                                                                                           aGCGGGGCTGGCCCCTTCG‑CGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCGGGTGATCCAct                >  1:17340/1‑149 (MQ=255)
                                                                                                                            gCGGGGCTGGCCCCTTCG‑CGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGNCCGGGTTCACCACATGCGGGTGATCCActc               <  1:35872/149‑1 (MQ=255)
                                                                                                                                       cccTTCG‑CGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCGGGTGATCCACTCTCaacaacaac    >  6:138633/1‑149 (MQ=255)
                                                                                                                                         cTTCG‑CGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCGGGTGATCCACTCTCAACAACAACgg  >  6:193410/1‑149 (MQ=255)
                                                                                                                                         cTTCG‑CGGGTAAACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCGGGTGATCCACTCTCAACAACAACgg  >  4:39161/1‑149 (MQ=255)
                                                                                                                                          ttCG‑CGGGTAAACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCGGGTGATCCACTCTCAACAACAACgg  >  3:87073/1‑148 (MQ=255)
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GCGGTTGTCGCCGCAGCTGGTCGACACCTATCCGGTGGAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCG‑CGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCGGGTGATCCACTCTCAACAACAACGG  >  NC_002947/1921402‑1921687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑