| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,975,827 | C→G | 5.2% | T253T (ACC→ACG) | pheA → | bifunctional chorismate mutase/prephenate dehydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,975,827 | 0 | C | G | 5.2% | 281.0 / 3.6 | 87 | T253T (ACC→ACG) | pheA | bifunctional chorismate mutase/prephenate dehydratase |
| Reads supporting (aligned to +/- strand): ref base C (39/43); new base G (2/2); total (41/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.38e-01 | |||||||||||
GTGCCGAAAGTGGCTGGACGCTCACTACCCGAACGTGGAGCGCGTGGCCGTTTCGAGCAATGC‑CGAAGCGGCCAAGCGGGTCAAGGGTGAGTGGAACTCGGCGGCGATCGCCGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGA‑TCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTG > NC_002947/1975683‑1975974 | gTGCCGAAAGTGGCTGGACGCTCACTACCCGAACGTGGAGCGCGTGGCCGTTTCGAGCAATGC‑CGAAGCGGCCAAGCGGGTCAAGGGTGAGTGGAACTCGGCGGCGCTCGCCGGCGATATGGCGGCCATCCTGTACGGCCTGACCCGTc > 4:64394/1‑149 (MQ=255) ccGAAAGTGGCTGGAAGCTCACTACCCGAACGTGGAGCTCGTGGCCGTTTCGAGCAATGC‑CGAAGCGGCCAAGCGGGTCAAGGGTGAGTGGAACTCGTCGGCGATCGCCGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTg < 5:377114/148‑1 (MQ=255) gAAAGTGGCTGGACGCTCACTACCCGAACGTGGAGCGCGTGGCCGTTTCGAGCAATGC‑CGAAGCGGCCAAGCGGGTCAAGGGTGAGTGGAACTCGGCGGCGATCGCCGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGcc > 3:210182/1‑149 (MQ=255) gTGGCTGGACGCTCACTACCCGAACGTGGAGCGCGTGGCCGTTTCGAGCAATGC‑CGAAGCGGTCAAGCGGGTCAAGGGTGAGTGGAACTCGGCGGCGATCGCCGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCgag > 7:240770/1‑148 (MQ=255) gCTGGACGCTCACTACCCGAACGTGGAGCGCGTGGCCGTTTCGAGCAATGC‑CGAAGCGGCCAAGCGGGTCAAGGGTGAGTGGAACTCGGCGGCGATCGCCGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGagaaga < 3:240498/149‑1 (MQ=255) gCTGGACGCTCACTACCCGAACGTGGAGCGCGTGGCCGTTTCGAGCAATGC‑CGAAGCGGCCAAGCGGGTCAAGGGTGAGTGGAACTCGGCGGCGATCGCCGGCGATATGGCGGCCAACCTGTACGGCCAGACGGGTCAGGCCgtacag < 6:296423/148‑6 (MQ=255) cTGGACGCTCACTACCCGAACGTGGAGCGCGTGGCCGTTTCGAGCAATGC‑CGAAGCGGCCAAGCGGGTCAAGGGTGAGTGGAACTCGGCGGCGATCGCCGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCgag > 4:33616/1‑144 (MQ=255) cTGGACGCTCACTACCCGAACGTGGAGCGCGTGGCCGTTTCGAGCAATGC‑CGAAGCGGCCAAGCGGGTCAAGGGTGAGTGGAACTCGGCGGCGATCGCCGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGA‑t < 2:117527/149‑1 (MQ=255) aCGCTCACTACCCGAACGTGGAGCGCGTGGCCGTTTCGAGCAATGC‑CGAAGCGGCCAAGCGGGTCAAGGGTGAGTGGAACTCGGCGGCGATCGCCGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGA‑TCGaa < 6:205378/149‑1 (MQ=255) aCGCTCACTACCCGAACGTGGAGCGCGTGGCCGTTTCGAGCAATGC‑CGAAGCGGCCAAGCGGGTCAAGGGTGAGTGGAACTCGGCGGCGATCGCCGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGA‑TCGaa < 1:327474/149‑1 (MQ=255) tCACTACCCGAACGTGGAGCGCGTGGCCGTTTCGAGCAATGC‑CGAAGCGGCCAAGCGGGTCAAGGGTGAGTGGAACTCGGCGGCGATCGCCGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGA‑TCGAAGAcc > 3:335845/1‑149 (MQ=255) aCTACCCGAACGTGGAGCGCGTGGCCGTTTCGAGCAATGC‑CGAAGCGGCCAAGCGGGTCAAGGGTGAGTGGAACTCGGCGGCGATCGCCGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGA‑TCGAAGACCGc > 7:290522/1‑149 (MQ=255) cTACCCGAACGTGGAGCGCGTGGCCGTTTCGAGCAATGC‑CGAAGCGGCCAAGCGGGTCAAGGGTGAGTGGAACTCGGCGGCGATCGCCGGCGATATGGCGGCCAACCTGTACGGCCAGACGGGTCAGGcc > 2:284647/1‑130 (MQ=255) cTACCCGAACGTGGAGCGCGTGGCCGTTTCGAGCAATGC‑CGAAGCGGCCAAGCGGGTCAAGGGTGAGTGGAACTCGGCGGCGATCGCCGGCGATATGGCGGCCAACCTGTACGGCCAGACGGGTCAGGcc < 1:284647/130‑1 (MQ=255) tACCCGAACGTGGAGCGCGTGGCCGTTTCGAGCAATGC‑CGAAGCGGCCAAGCGGGTCAAGGGTGAGTGGAACTCGGCGGCGATCGCCGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGA‑TCGAAGACCGccc > 3:2666/1‑149 (MQ=255) cccGAACGTGGAGCGCGTGGCCGTTTCGAGCAATGC‑CGAAGCGGTCAAGCGGGTCAAGGGTGAGTGGAACTCGGCGGCGATCGCCGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGA‑TCGAAGACCGccc < 8:240770/147‑1 (MQ=255) cccGAACGTGGAGCGCGTGGCCGTTTCGAGCAATGC‑CGAAGCGGCCAAGCGGGTCAAGGGTGAGTGGAACTCGGCGGCGATCGCCGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGA‑TCGAAGACCGCCCgg > 8:298008/1‑149 (MQ=255) cccGAACGTGGAGCGCGTGGCCGTTTCGAGCAATGC‑CGAAGCGGCCAAGCGGGTCAAGGGTGAGTGGAACTCGGCGGCGATCGCCGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGA‑TCGAAGACCGCCCg > 4:198586/1‑148 (MQ=255) ccGAACGTGGAGCGCGTGGCCGTTTCGAGCAATGC‑CGAAGCGGCCAAGCGTGTCAAGGGTGAGTGGAACTCGGCGGCGATCGCCGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGA‑TCGAAGACCGCCCGGa < 8:212163/149‑1 (MQ=255) ccGAACGTGGAGCGCGTGGCCGTTTCGAGCAATGC‑CGAAGCGGCCAAGCGGGTCAAGGGTGAGTGGAACTCGGCGGCGATCGCCGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGA‑TCGAAGACCGCCCGGa > 6:359956/1‑149 (MQ=255) cGAACGTGGAGCGCGTGGCCGTTTCGAGCAATGC‑CGAAGCGGCCAAGCGGGTCAAGGGTGAGTGGAACTCGGCGGCGATCGCCGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGA‑TCGAAGACCGCCCGGa > 6:191432/1‑148 (MQ=255) cGAACGTGGAGCGCGTGGCCGTTTCGAGCAATGC‑CGAAGCGGCCAAGCGGGTCAAGGGTGAGTGGAACTCGGCGGCGATCGCCGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGA‑TCGAAGACCGCCCGGAc < 7:97192/149‑1 (MQ=255) cGAACGTGGAGCGCGTGGCCGTTTCGAGCAATGC‑CGAAGCGGCCAAGCGGGTCAAGGGTGAGTGGAACTCGGCGGCGATCGCCGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGA‑TCGAAGACCGCCCGGAc < 2:73708/149‑1 (MQ=255) cGAACGTGGAGCGCGTGGCCGTTTCGAGCAATGC‑CGAAGCGGCCAAGCGGGTCAAGGGTGAGTGGAACTCGGCGGCGATCGCCGGCGAGATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGA‑TCGAAGACCGCCCGGa > 8:63628/1‑148 (MQ=255) gAACGTGGAGCGCGTGGCCGTTTCGAGCAATGC‑CGAAGCGGCCAAGCGGGTCAAGGGTGAGTGGAACTCGGCGGCGATCGCCGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGA‑TCGCAGACCGCCCGGAc > 5:196825/1‑148 (MQ=255) aaCGTGGAGCGCGTGGCCGTTTCGAGCAATGC‑CGAAGCGGCCAAGCGGGTCAAGGGTGAGTGGAACTCGGCGGCGATCGCCGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGA‑TCGAAGACCGCCCGGACaa < 3:95454/149‑1 (MQ=255) aCGTGGAGCGCGTGGCCGTTTCGAGCAATGC‑CGAAGCGGCCAAGCGGGTCAAGGGTGAGTGGAACTCGGCGGCGATCGCCGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGA‑TCGAAGACCGCCCGGACAAc < 7:173506/149‑1 (MQ=255) aCGTGGAGCGCGTGGCCGTTTCGAGCAATGC‑CGAAGCGGCCAAGCGGGTCAAGGGTGAGTGGAACTCGGCGGCGATCGCCGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGA‑TCGAAGACCGCCCGGACAAc < 7:93784/149‑1 (MQ=255) tagagcgTGGCCGTTTCGTGCAATGC‑CGATGCGGCCAAGCGGTGCAAGTGTGAGTGGAACTCGGCGGCGATCGCCGGCGATATGGCGGCCAACCTGTACGGCCTGTCCCGTCTGGCCGAGAAGA‑TCGAAGACCGCCCGGACAACTCCa < 5:42939/144‑1 (MQ=255) gcgcGTGGCCGTTTCGAGCAATGC‑CGAAGCGGCCAAGCGGGTCAAGGGTGAGTGGAACTCGGCGGCGATCGCCGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGA‑TCGAAGACCGCCCGGACAACTCCAcgc < 4:306402/149‑1 (MQ=255) cgcgTGGCCGTTTCGAGCAATGC‑CGAAGCGGCCAAGCGGGTCAAGGGTGAGTGGAACTCGGCGGCGATCGCCGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGA‑TCGAAGACCGCCCGGACAACTCCAcgcg < 8:290522/149‑1 (MQ=255) gcttGGCCGCTTCG‑GCATTGCTCGAAACGGCCAAGCGGGTCAAGGGTGAGTGGAACTCGGCGGCGATCGCCGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGA‑TCGAAGACCGCCCGGACAACTCCACgcgc > 3:18067/4‑149 (MQ=255) gTGGCCGTTTCGAGCAATGC‑CGAAGCGGCCAAGCGGGTCAAGGGTGAGTGGAACTCGGCGGCGATCGCCGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGA‑TCGAAGACCGCCCGGACAACTCCACGCGCtt > 1:286766/1‑149 (MQ=255) ccGTTTCGAGCAATGC‑CGAAGCGGCCAAGCGGGTCAAGGGTGAGTGGAACTCGGCGGCGATCGCCGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGA‑TCGAAGACCGCCCGGACAACTCCACGCGCTTCCtg < 7:298008/149‑1 (MQ=255) ccGTTTCGAGCAATGC‑CGAAGCGGCCAAGCGGGTCAAGGGTGAGTGGAACTCGGCGGCGATCGCCGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGA‑TCGAAGACCGCCCGGACAACTCCACGCGCTTCCt > 6:20853/1‑148 (MQ=255) ccGTTTCGAGCAATGC‑CGAAGCGGCCAAGCGGGTCAAGGGTGAGTGGAACTCGGCGGCGATCGCCGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGA‑TCGAAGACCGCCCGGACAACTCCACGCGCTTCCt > 6:211169/1‑148 (MQ=255) cGTTTCGAGCAATGC‑CGAAGCGGCCAAGCGGGTCAAGGGTGAGTGGAACTCGGCGGCGATCGCCGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGA‑TCGAAGACCGCCCGGACAACTCCACGCGCTTCCtga > 5:322253/1‑149 (MQ=255) cGTTTCGAGCAATGC‑CGAAGCGGCCAAGCGGGTCAAGGGTGAGTGGAACTCGGCGGCGATCGCCGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGA‑TCGAAGACCGCCCGGACAACTCCACGCGCTTCCtga > 5:177234/1‑149 (MQ=255) cGTTTCGAGCAATGC‑CGAAGCGGCCAAGCGGGTCAAGGGTGAGTGGAACTCGGCGGCGATCGCCGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGA‑TCGAAGACCGCCCGGACAACTCCACGCGCTTCCtga < 2:330745/149‑1 (MQ=255) cGTTTCGAGCAATGC‑CGAAGCGGCCAAGCGGGTCAAGGGTGACTGGAACTCGGCGGCGATCGCCGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGA‑TCGAAGACCGCCCGGACAACTCCACGCGCTTCCtga > 5:276337/1‑149 (MQ=255) tttCGAGCAATGC‑CGAAGCGGCCAAGCGGGTCAAGGGTGAGTGGAACTCGGCGGCGATCGCCGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGA‑TCGAAGACCGCCCGGACAACTCCACGCGCTTCCtgatg < 4:335845/149‑1 (MQ=255) gAGCAATGC‑CGAAGCGGTCAAGCGGGTCAAGGGTGAGTGGAACTCGGCGGCGATCGCCGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGA‑TCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATc < 5:42642/148‑1 (MQ=255) gAGCAATGC‑CGAAGCGGCCAAGCGGGTCAAGGGTGAGTGGAACTCGGCGGCGATCGCCGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGA‑TCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATc < 5:280568/148‑1 (MQ=255) gAGCAATGC‑CGAAGCGGCCAAGCGGGTCAAGGGTGAGTGGAACTCGGCGGCGATCGCCGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGA‑TCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCg < 2:286766/149‑1 (MQ=255) gCAATGC‑CGAAGCGGCCAAGCGGGTCAAGGGTGAGTGGAACTCGGCGGCGATCGCCGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGGCGAGAAGA‑TCGAAGACCGCCCGGTCAACTCCACGCGCTTCCTGATGATCGGt < 2:226986/149‑1 (MQ=255) aaTGC‑CGAAGCGGCCAAGCGGGTCAAGGGTGAGTGGAACTCGGCGGCGATCGCCGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGA‑TCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTaa > 3:251042/1‑149 (MQ=255) aTGC‑CGAAGCGGCCAAGCGGGTCAAGGGTGAGTGGAACTCGGCGGCGATCGCCGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGA‑TCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAAc > 3:368246/1‑149 (MQ=255) gC‑CGAAGCGGCCAAGCGGGTCAAGGGTGAGTGGAACTCGGCGGCGATCGCCGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGA‑TCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAAc > 8:312443/1‑147 (MQ=255) aaGCGGCCAAGCGGGTCAAGGGTGAGTGGAACTCGGCGGCGATCGCCGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGA‑TCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGaa < 2:289824/149‑1 (MQ=255) aaGCGGCCAAGCGGGTCAAGGGTGAGTGGAACTCGGCGGCGATCGCCGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGA‑TCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGaa < 5:134493/149‑1 (MQ=255) aaGCGGGTCAAGGGTGAGTGGAACTCGGCGGCGATCGCCGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGA‑TCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTAccgcc < 6:177234/149‑1 (MQ=255) aaGGGTGAGTGGAACTCGGCGGCGATCGCCGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGA‑TCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGcga < 3:198586/149‑1 (MQ=255) aaGGGTGAGTGGAACTCGGCGGCGATCGCCGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGA‑TCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGcga < 5:274743/149‑1 (MQ=255) aGGGTGAGTGGAACTCGGCGGCGATCGCCGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGA‑TCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGcgac < 8:298752/149‑1 (MQ=255) ggTGAGTGGAACTCGGCGGCGATCGCCGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGA‑TCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGcgacga < 4:251042/149‑1 (MQ=255) tGAGTGGAACTCGGCGGCGATCGCCGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGA‑TCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACa < 3:33616/149‑1 (MQ=255) aGTGGAACTCGGCGGCGATCGCCGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGA‑TCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCgacgac > 1:49202/1‑146 (MQ=255) aGTGGAACTCGGCGGCGAGCGCGTGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGA‑GCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACGGGCGACGACaa < 3:64394/148‑1 (MQ=255) gAACTCGGCGGCGATCGCCGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGA‑TCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCt > 1:176982/1‑149 (MQ=255) aaCTCGGCGGCGATCGCCGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGA‑TCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTc > 7:232560/1‑149 (MQ=255) cTCGGCGGCGATCGCCGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGA‑TCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGa < 2:176982/149‑1 (MQ=255) gATCGCCGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGA‑TCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGttt < 6:276337/149‑1 (MQ=255) gCCGGCGATATGGCGGCCAACCTGTACGGCCTGACTCGTCTGGCCGAGAAGA‑TCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGa < 5:170298/148‑1 (MQ=255) ccGGCGATATGGCGGCCAACCTGTACGGGCTGACCCGTCTGGCCGAGAAGA‑TCGAAGACCGCCCGGACAACTCCACGCGCTTcc > 1:216163/1‑84 (MQ=255) ccGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGA‑TCGAAGACCGCCCGGACAACTCCACGCGCTTcc < 2:216163/84‑1 (MQ=255) ccGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGA‑TCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGAt > 7:116743/1‑148 (MQ=255) ccGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGA‑TCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGAt > 4:370868/1‑148 (MQ=255) cGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGA‑TCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATTATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGa < 4:2666/149‑1 (MQ=255) gCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGA‑TCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGc < 6:322253/149‑1 (MQ=255) gATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGA‑TCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGcaa > 8:272381/1‑149 (MQ=255) tGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGA‑TCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGcaacaa < 6:196825/148‑1 (MQ=255) tGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGA‑TCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGcaacaa < 5:359956/148‑1 (MQ=255) ggcggcCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGA‑TCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGc > 7:105615/1‑149 (MQ=255) ggcggcCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGA‑TCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGc > 2:56912/1‑149 (MQ=255) ggcCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGA‑TCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAg < 5:211169/149‑1 (MQ=255) cAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGAGTCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGt > 1:247385/1‑149 (MQ=255) cAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGA‑TCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTg > 7:72758/1‑149 (MQ=255) cAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGA‑TCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCCATGAGCAACAAGCCAGGTg > 2:204466/1‑149 (MQ=255) ccTGTACGGCCTGACCCGTCTGGCCGAGTAGA‑TCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGt > 1:212478/1‑149 (MQ=255) ccTGTACGGCCTGACCCGTCTGGCCGAGAAGA‑TCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGt > 5:286253/1‑149 (MQ=255) cTGTACGGCCTGACCCGTCTGGCCGAGAAGA‑TCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGtt > 2:359006/1‑149 (MQ=255) cTGTACGGCCTGACCCGTCTGGCCGAGAAGA‑TCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGtt > 8:118693/1‑149 (MQ=255) tctcGGCCAGACGGGTCAGGCCGAGAAGA‑TCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTg > 6:154084/4‑148 (MQ=255) tACGGCCTGACCCGTCTGGCCGAGAAGA‑TCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGc < 6:286253/148‑1 (MQ=255) ccTGACCCGTCTGGCCGAGAAGA‑TCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGt < 5:20853/149‑1 (MQ=255) ccTGACCCGTCTGGCCGAGAAGA‑TCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGt < 4:368246/149‑1 (MQ=255) aCCCGTCTGGCCGAGAAGA‑TCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGc < 1:229322/148‑1 (MQ=255) cccGTCTGGCCGAGAAGA‑TCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATTATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTg < 1:78562/149‑1 (MQ=255) cccGTCTGGCCGAGAAGA‑TCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCt < 7:312443/148‑1 (MQ=255) | GTGCCGAAAGTGGCTGGACGCTCACTACCCGAACGTGGAGCGCGTGGCCGTTTCGAGCAATGC‑CGAAGCGGCCAAGCGGGTCAAGGGTGAGTGGAACTCGGCGGCGATCGCCGGCGATATGGCGGCCAACCTGTACGGCCTGACCCGTCTGGCCGAGAAGA‑TCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTG > NC_002947/1975683‑1975974 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |