Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,976,789 C→G 11.0% R151R (CGC→CGG)  PP_1770 → 3‑phosphoshikimate 1‑carboxyvinyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,976,7890CG11.0% 144.1 / 7.1 53R151R (CGC→CGG) PP_17703‑phosphoshikimate 1‑carboxyvinyltransferase
Reads supporting (aligned to +/- strand):  ref base C (23/24);  new base G (3/3);  total (26/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

ATCACCGACGTCGGCAGCGCCAAGGGCAACGTCGTGCGTGAGGCGCGTGCCGTCTTCGGTGCCCGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTGTGGCGTGCGCTGGACGCCGATGTGGAGCACATGTCGGTCGAGCGCCATGACGAAGTACTGGCCGCCACCAGCCATCTGC  >  NC_002947/1976643‑1976937
                                                                                                                                                  |                                                                                                                                                    
atcaCCGACGTCGGCAGCGCCAAGGGCAACGTCGTGCGTGAGGCGCGTGCCGTCTTCGGTGCCCGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTccgccg                                                                                                                                                    >  1:148885/1‑149 (MQ=255)
atcaCCGACGTCGGCAGCGCCAAGGGCAACGTCGTGCGTGAGGCGCGTGCCGTCTTCGGTGCCCGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTccgccg                                                                                                                                                    <  8:194479/149‑1 (MQ=255)
          tCGGCAGCGCCAAGGGCAACGTCGTGCGTGAGGCGCGTGCCGTCTTCGGTGCCCGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTGCCGGCGGAACAAGGtg                                                                                                                                          <  1:18286/149‑2 (MQ=255)
          tCGGCAGCGCCAAGGGCAACGTCGTGCGTGAGGCGCGTGCCGTCTTCGGTGCCCGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTGCCGGCGGAACAAGGtg                                                                                                                                          >  1:236249/1‑148 (MQ=255)
               aGCGCCAAGGGCAAGGTGGTGCGTGAGGCGCGTGCCGTCTTCGGTTCCCGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTGCCGGCGGAACAAGGtggcatt                                                                                                                                     <  2:236249/149‑7 (MQ=255)
               aGCGCCAAGGGCAACGTCGTGCGTGAGGCGCGTGCCGTCTTCGGTGCCCGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCt                                                                                                                                     <  6:289164/149‑1 (MQ=255)
                            aCGTCGTGCGTGAGGCGCGTGCCGTCTTCGGTGCCCGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGcc                                                                                                                        <  6:296039/149‑1 (MQ=255)
                              gtcgtGCGTGAGGCGCGTGCCGTCTTCGGTGCCCGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCg                                                                                                                       >  2:142102/1‑148 (MQ=255)
                                         ggCGCGTGCCGTCTTCGGTGCCCGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCccg                                                                                                           >  8:109417/1‑149 (MQ=255)
                                          gcgcGTGCCGTCTTCGGTGCCCGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCccgc                                                                                                          <  7:108294/149‑1 (MQ=255)
                                          gcgcGTGCCGTCTTCGGTGCCCGCCTGCCGCGCTTCGTACCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGACACCTTGTTCCGCCGGCACAAGGTAATCCTCACGCCGCTGGCCGAAAACGACCccgc                                                                                                          >  5:163536/1‑149 (MQ=255)
                                                 ccGTCTTCGGTGCCCGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACCAGGTCATCCTCACGCCGCTGGCCGAAACCGCCCCCGCCGCGCTg                                                                                                   >  3:25016/1‑149 (MQ=255)
                                                      ttCGGTGCCCGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCt                                                                                              >  7:126139/1‑149 (MQ=255)
                                                       tCGGTGCCCGCCTGCCGCGCTTCGTCCCGTGCCACCCAATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCt                                                                                              <  6:163536/148‑1 (MQ=255)
                                                        cGGTGCCCGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAAc                                                                                                                   <  8:87981/126‑1 (MQ=255)
                                                        cGGTGCCCGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAAc                                                                                                                   >  7:87981/1‑126 (MQ=255)
                                                         ggTGCCCGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACATGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTgg                                                                                            <  1:284261/148‑1 (MQ=255)
                                                          gTGCCCGCCTGCCGCGCTTCGTCCCGGGCCAGCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGtt                                                                                          <  7:245339/149‑1 (MQ=255)
                                                             cccGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGAc                                                                                       <  1:142102/149‑1 (MQ=255)
                                                              ccGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGAcc                                                                                      >  6:217296/1‑149 (MQ=255)
                                                              ccGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTAGTTCCGCCGGCACAAGGTCATCCTCCCGCCGCTGGCCGAACCCGCCCCCGCCGCGCTCGTCCTGGTTGAcc                                                                                      >  7:205726/1‑149 (MQ=255)
                                                               cGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCg                                                                                     <  2:164218/149‑1 (MQ=255)
                                                                 ccTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGcc                                                                                   >  3:120955/1‑149 (MQ=255)
                                                                      cgcgCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTGTgg                                                                              >  2:30623/1‑149 (MQ=255)
                                                                       gcgcTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTGTGGc                                                                             <  4:131951/149‑1 (MQ=255)
                                                                       gcgcTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTGTGGc                                                                             <  8:354014/149‑1 (MQ=255)
                                                                              gTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTGTGGCGTGCGCt                                                                      <  8:119837/149‑1 (MQ=255)
                                                                                 ccGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTGTGGCGTGCGCTGGa                                                                   >  2:25046/1‑149 (MQ=255)
                                                                                     gCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTGTGGCGTGCGCTGGACGcc                                                               >  5:6008/1‑149 (MQ=255)
                                                                                        aCCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTGTGGCGTGCGCTGGACGCCGAt                                                            >  6:357077/1‑149 (MQ=255)
                                                                                          cccATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTGTGGCGTGCGCTGGACGCCGAtgt                                                          >  4:363744/1‑149 (MQ=255)
                                                                                          cccATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTGCCGGCGGAACAAg                                                                                                                                             <  4:196634/66‑1 (MQ=38)
                                                                                          cccATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTGCCGGCGGAACAAg                                                                                                                                             >  3:196634/1‑66 (MQ=38)
                                                                                             aTCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTGTGGCGTGCGCTGGACGCCGAtgtg                                                         >  7:173066/1‑147 (MQ=255)
                                                                                             aTCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTGTGGCGTGCGCTGGACGCCGATGTGGa                                                       <  6:320435/149‑1 (MQ=255)
                                                                                              tCGCCGGTTCCGCGCAGAGCGGGGTTGAGGCGTCCAATGCCACCTTGTTCGGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGATACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTGTGGCGTGCGCTGGACGCCGATGTGGAg                                                      <  8:205726/149‑1 (MQ=255)
                                                                                                  cGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTGTGGCGTGCGCTGGACGCCGATGTGGAGcaca                                                  >  6:302594/1‑149 (MQ=255)
                                                                                                   ggTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATc                                                                                                                                       <  4:304673/63‑1 (MQ=255)
                                                                                                   ggTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATc                                                                                                                                       >  3:304673/1‑63 (MQ=255)
                                                                                                   ggTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTGTGGCGTGCGCTGGACGCCGATGTGGAGCACAt                                                 <  3:363744/149‑1 (MQ=255)
                                                                                                    gTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTGTGGCGTGCGCTGGACGCCGATGTGGAGCACATg                                                <  4:105113/149‑1 (MQ=255)
                                                                                                        cGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTgg                                                                                                  >  5:121522/1‑95 (MQ=255)
                                                                                                        cGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTgg                                                                                                  <  6:121522/95‑1 (MQ=255)
                                                                                                        cGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTGTGGCGTGCGCTGGACGCCGATGTGGAGCACATgtcg                                             <  5:302594/148‑1 (MQ=255)
                                                                                                         gAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTGTGGCGTGCGCTGGACGCCGATGTGGAGCACATgtcggt                                           >  7:184902/1‑149 (MQ=255)
                                                                                                         gAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTGTGGCGTGCGCTGGACGCCGATGTGGAGCACATgtcggt                                           <  7:109417/149‑1 (MQ=255)
                                                                                                         gAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTGTGGCGTGCGCTGGACGCCGATGTGGAGCACATgtcggt                                           >  3:52241/1‑149 (MQ=255)
                                                                                                            cAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTGTGGCGTGCGCTGGACGCCGATGTGGAGCACATgtcggtc                                          >  7:283664/1‑147 (MQ=255)
                                                                                                               agCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTGTGGCGTGCGCTGGACGCCGATGTGGAGCACATGTCGGTCGAgcg                                     <  8:126139/149‑1 (MQ=255)
                                                                                                                              tCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTGTGGCGTGCGCTGGACGCCGATGTGGAGCACATGTCGGTCGAGCGCCATGACGAAGTACt                      >  5:54530/1‑149 (MQ=255)
                                                                                                                              tCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTGTGGCGTGCGCTGGACGCCGATGTGGAGCACATGTCGGTCGAGCGCCATGACGAAGTACt                      <  8:283664/149‑1 (MQ=255)
                                                                                                                                cAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTGTGGCGTGCGCTGGACGCCGATGTGGAGCACATGTCGGTCGAGCGCCATGACGAAGTACTg                     <  6:6008/148‑1 (MQ=255)
                                                                                                                                   tGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTGTGGCGTGCGCTGGACGCCGATGTGGAGCACATGTCGGTCGAGCGCCATGACGAAGTACTg                     <  4:353967/145‑1 (MQ=255)
                                                                                                                                   tGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTGTGGCGTGCGCTGGACGCCGATGTGGAGCACATGTCGGTCGAGCGCCATGACGAAGTACTg                     >  3:353967/1‑145 (MQ=255)
                                                                                                                                        ccTTGTGCCGGCGGAACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTGTGGCGTGCGCTGGACGCCGATGTGGAGCACATGTCGGTCGAGCGCCATGACGAAGTACTGGCCGccac             >  2:33000/1‑148 (MQ=255)
                                                                                                                                                  ccgGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTGTGGCGTGCGCTGGACGCCGATGTGGAGCACATGTCGGTCGAGCGCCATGACGAAGTACTGGCCGCCACCAGCCATCTGc  <  1:150838/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
ATCACCGACGTCGGCAGCGCCAAGGGCAACGTCGTGCGTGAGGCGCGTGCCGTCTTCGGTGCCCGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTGTGGCGTGCGCTGGACGCCGATGTGGAGCACATGTCGGTCGAGCGCCATGACGAAGTACTGGCCGCCACCAGCCATCTGC  >  NC_002947/1976643‑1976937

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑